JP2005232066A - Ceramic nanoparticle, colloidal water dispersion including the same, and method for production thereof - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a ceramic nanoparticle or a colloidal water dispersion including the same which is a medical effective ingredient for effectively exerting a free radical erasing performance against the free radicals in living bodies, particularly hydrogen peroxide being a free radical generation source, and which exerts an innovative effect in its use in various fields, and also to provide a method for production thereof. <P>SOLUTION: The ceramic nanoparticle contains a ceramic microparticle body comprising silicon carbide and/or its unreacted material of at least one selected from the group consisting of silicon dioxide, silicon monoxide and metal carbides. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

(Definition)
1, “Ceramic particle” in this specification refers to a substance made of silicon carbide and its unreacted substance, and [nano ceramic fine particle] means a fine particle made of a nano-shaped ceramic.
2. In this specification, “nanocolloid water” refers to a substance composed of colloidal water including [ceramic particles] and [nanoceramic particles].

Technical field to which the invention belongs

  The present invention relates to nanoceramic fine particles and nanocolloid water that can efficiently exhibit radical scavenging suppressing action on in vivo radicals and a method for producing the same.

  In addition, the application field of the present invention is wide and is the following field even if it has been clarified at present. However, the present invention is not limited to this, and exhibits excellent effects in all fields along with human life. It fully includes the possibility of being used in a certain field.

  For example, the present invention is in the field of medicine, and can prevent the occurrence of various cancers such as colorectal cancer, small intestine cancer and skin cancer, brain diseases, neurological diseases, cerebrovascular disorders, lung diseases, gastrointestinal mucosa It has excellent effects on disorders, liver diseases, kidney diseases, eye diseases, skin diseases, diabetes diseases, and immune diseases. In other fields, such as lifestyle diseases, it prevents diabetes, cell damage, skin aging, and prevents the spread of lipid peroxide and neutral fat.

  It also cleans blood vessels and suppresses the increase in cholesterol and prevents hypertension, thereby preventing myocardial infarction, angina pectoris, cerebral infarction, intracerebral hemorrhage, and anti-ulcer. In addition, the topic of hay fever recently, lifestyle diseases such as atopy and stress relief, and the adverse effects of electromagnetic waves will be suppressed to prevent insomnia and coldness.

  In particular, in the application and application field of the nanoceramic fine particles and nanocolloidal water of the present invention, they can be mixed with water or the like as a medicine for drinking, and can also be used as powdered tablets or softening agents such as PH regulators.

Moreover, even if it is mixed in so-called drinks and soft drinks as health drinks, it can be used for various health foods as a fragrance for various foods for humans, livestock and pets, and as an additive for preparations containing colorants. Also good.
This is also optimal as an improved fertilizer for the soil for growing various plants.

In addition, various health goods have recently been deceived, but for example, it is better to weld, affix or embed it on decorative items such as bracelets, rings, necklaces, earrings and earrings that touch the body directly. .
Furthermore, it is also effective to add the nanoceramic fine particles and nanocolloid water of the present invention during the process of the cloth used for clothes or the like.

  Subsequently, the occurrence of various germs and fungi has become a hot topic recently, but since the antioxidant of the present invention has an antibacterial action, it can be applied to inks of printed matter including various credit cards, or applied onto the printed matter. It has been reported that this method has an excellent antibacterial effect.

  In addition, the nano-ceramic fine particles and nano-colloidal water of the present invention were most suitable for mixing with various cosmetics and bathing agents, and the color became white (skin). It has been reported. In addition, it is possible to prevent such occurrences in applications where resin bottles, beer cans (bottles), window glass including the front of automobiles, mirrors, room walls, furniture, and so-called condensation are generated. Has been pointed out.

Conventional technology

  The in vivo radicals include radicals generated as redox metabolic intermediates, radicals generated by ultraviolet rays, radiation, tap water chlorine, some pesticides, some pharmaceuticals, photocatalytic activity positive cosmetics, tobacco, etc. As typical radicals, the presence of active oxygen such as superoxide anion (O 2−) and hydroxyl radical (OH *) is known.

  In addition to biological defense mechanisms against viruses and pathogens, active oxygen plays an essential role in hormone biosynthesis and gene expression, but on the other hand, it itself, like superoxide anion and hydroxyl radical. When excessive active oxygen that is a radical generation source such as hydrogen peroxide or singlet oxygen is generated, it reacts with biological components such as lipids, proteins, and nucleic acids, resulting in damage to cell membranes. It is known that protein denaturation, gene mutation, and the like cause damage to biological functions and cause various diseases such as cancer and aging.

  Examples of such diseases that involve active oxygen include inflammatory cell disorders, diabetic diseases (nephropathy, retinopathy, neuropathy), ischemia / reperfusion injury, renal failure, rheumatoid arthritis, myocardium There is an infarction.

A substance having such a property of capturing and stabilizing a radical in the living body is called a radical scavenger. In the living body, as a substance that controls the generation of active oxygen, superoxide dismutase (SOD), catalase, Vitamin C, vitamin E, carotenoids, uric acid, bilirubin, etc. can act as radical scavengers as enzymes that trap glutathione peroxynase (GSH-PX) and active oxygen to inhibit chain reactions Are known. In reagents other than in-vivo substances, it is known that isoflavonoids, phenols, ascorbic acid and the like have an erasing action on active oxygen.

  A substance having such a property of capturing and stabilizing radicals in the living body is called a radical scavenger. In the living body, substances that control the generation of active oxygen include superoxide dismutase (SOD), catalase, Vitamin C, vitamin E, carotenoids, uric acid, bilirubin, etc. may act as radical scavengers as an enzyme such as glutathione peroxynase (GSH-PX), or as a physical property that traps active oxygen and inhibits chain reactions. Are known.

  In reagents other than in-vivo substances, isoflavonoids, phenols, ascorbic acid, etc. are known to have a scavenging action on active oxygen. It is highly reactive and easily reacts with components such as lipids, nucleic acids, and proteins adjacent to each other in the living body, resulting in oxidative damage that leads to various diseases. Oxidized by superoxide, a kind of reactive oxygen species, forms foam cells, causing arteriosclerosis.

Moreover, the production of hydroxy radicals brought about by irradiation of radiation gives serious damages such as carcinogenesis to the living body.

As the toxicity of such reactive oxygen species to the living body becomes clear, antioxidants such as reactive oxygen species-eliminating substances having the activity of efficiently eliminating these active oxygen species are included in the living body or in food, pharmaceuticals, agriculture, etc. It is useful as a protective agent against oxidative degradation of ingredients, and is expected to be used practically in the food industry, particularly processed fishery products, health foods, nutritional foods, as well as in the pharmaceutical / agricultural and cosmetic fields. (Non-Patent Document 1)

As an example, a conventional external preparation for skin is a skin external preparation containing an extract obtained from an olive plant (excluding leaves), and the extract is contained as a beautifying skin component, particularly as an anti-aging component and / or a whitening component. The extract can be obtained by extracting a product obtained in the olive plant and / or olive oil production process with water and / or an organic solvent, and further extracting the extract with a concentration moat and / or fractionation / purification treatment. Thus, each effect is reinforced, and an invention (Patent Document 1) has been made for use as a skin beautifying agent containing the extract as an active ingredient, particularly as an anti-aging agent and whitening agent for skin.

Similarly, as a water-containing cosmetic, effective as a water-containing cosmetic for reducing skin inflammatory reaction caused by skin peroxidation, and improving skin condition (skin wrinkles, firmness), selenium compounds and / or A plant extract containing a selenium compound is a water-containing cosmetic containing 0.00001 to 0.1% by weight as an equivalent amount of selenium and 40% by weight or more of water as essential components. Inventions of solubilized systems, oil-in-water emulsions, water-in-oil emulsions, and water-containing cosmetics exhibiting a viscous or gel-like form have been made (Patent Document 2).

  Subsequently, a pharmaceutical composition for treating or inhibiting a disease state induced by free radicals is an invention comprising an antioxidant amount of 8,9-dehydroestrone or a pharmaceutically acceptable 3-sulfate ester salt thereof. (Patent Document 3).

However, among those currently on the market and already invented, for example, inflammatory cell disorders, diabetic diseases (nephropathy, retinopathy, neuropathy), ischemia / reperfusion disorders, renal failure, rheumatic The effect of preventing or treating diseases associated with active oxygen such as arthritis and myocardial infarction is still insufficient, and in particular, the appearance of substances having significant effects in the above-mentioned fields has not been proposed.
JP 2001-181198 (abstract) JP 5-9108 (abstract) JP-A-9-169650 (abstract) Halliwell B. and Gutterridge MC BIOCHEM.J. 219,1-1 4, (1984))

Problems invented

  The subject of the present invention is an active ingredient of a medicine that can effectively exert radical scavenging ability against in vivo radicals, particularly hydrogen peroxide as a radical generation source, and is used in almost every field. In this field, nano ceramic fine particles and nano colloidal water exhibiting an epoch-making effect are provided, and a production method thereof is proposed.

  In general, the characteristics of the present invention are nano-ceramic fine particles including a ceramic fine particle main body made of silicon carbide and / or an unreacted substance thereof, and in particular, the unreacted substances (silicon dioxide, silicon monoxide, metal carbide, etc.) It is.

  Thus, as one of the preferred embodiments of the present invention, there is provided the antioxidant of the nano ceramic fine particles and nano colloidal water, wherein the radical to be captured is active oxygen, preferably hydrogen peroxide. It can be used for applications such as the above-mentioned antioxidants for pharmaceutical active ingredients, pharmaceutical additives used for pharmaceutical formulations, foods, food additives, cosmetics, and bathing agents. That is, the medicament containing the antioxidant of the present invention as an active ingredient is useful for the prevention and / or treatment of diseases involving in vivo radicals.

  Further, the nanoceramic fine particles and nanocolloid water of the present invention obtained by the production method thereof and used as an antioxidant is a radical scavenging method in vivo, including an effective amount of nanoceramic fine particles and nanoceramic fine particles. The method comprising the step of administering colloidal water to a mammal, including humans, is a method for preventing / treating a disease further involving in vivo radicals, wherein the nanoceramic fine particles have an effective amount for prevention and / or treatment, and nanoceramics A method comprising the step of administering colloidal water containing microparticles to mammals including humans is also a feature of the invention.

  In this case, it is also a feature of the present invention that the radical to be captured is one or more selected from active oxides such as superoxide, hydrogen peroxide, hiddlexyl radical, and singlet oxygen.

  Further, the size of the nanoceramic body is 0.3 microns or less, has ferromagnetism, and has a predetermined kiloohm conductivity, which is also a feature of the present invention.

  Subsequently, it is also a feature of the present invention to produce nanoceramic colloidal water by dissolving the nanoceramic fine particle main body in normal water or the like and filtering, and also forming the material into a spherical shape. As a result, it is convenient and effective for use in various diseases and prevention or treatment, and this point is also a feature of the present invention.

  For example, in the traditional medicine at present, fine silica (carbon dioxide) of about 1 micron is good for burns, and the effect of suppressing diarrhea has been found. One of the main constitutions of the present invention is that nano-level microparticles (carbon-reduced nanoceramics) perform various pharmacological actions in a living body in a nanocolloid state.

  Therefore, the nano ceramic fine particle main body and / or nano nano colloid water main body of the present invention can be used as an analgesic agent, anti-inflammatory agent, anticancer agent, aldose reductase inhibitor for diabetic complications, disaccharide-degrading enzyme inhibitor, melanin production inhibitor, It is also characterized by its use as an antiallergic agent.

  In addition, for example, this colloidal water has been confirmed to have an effect of lowering blood glucose level. The mechanism is known to inhibit disaccharide-degrading enzymes. Thus, this antioxidant action is a physical action, and its mechanism is to suppress the weight of lipid peroxide, so that O in the hydrogen peroxide is captured, so it can be said that it has an antioxidant action. For example, because it can effectively prevent tissue cell damage caused by free radicals, it can prevent skin aging, suppress skin cancer, and the like.

  On the other hand, since anti-inflammatory action, analgesic action, anti-cancer action, etc. are physiological actions, there are various mechanisms for expressing these actions. As a medical, therapeutic, prophylactic, and palliative, it has been found to have significant effects.

  Furthermore, the anti-oxidation action of the present invention cured the coldness. I'm less tired. As a result of repeated clinical experiments such as relief of shortness of breath, it is easy to make medical inferences from these experimental data. That is, for example, the antioxidant effect and the less fatigue are easily combined.

Mode of implementation

  The scope of application of the present invention is the following fields even if it is currently known, but it is not limited to this, and it exhibits excellent effects in all fields along with human life, and can be used in each field. The present invention is also considered to be within the technical category of the present invention. For example, a representative example of a disease involving active oxygen of the present invention is to prevent various cancers from occurring in advance, in particular to prevent colon cancer, small intestine cancer, and skin cancer. Proven in

  It also has excellent effects on various brain diseases, especially neurological diseases (Alzheimer's disease, Parkinson's disease, cerebrovascular disease, etc.), and pulmonary diseases (pulmonary oxygen poisoning, respiratory stimulant group, airway hypersensitivity, etc.) ), Gastrointestinal mucosal disorders (gastrointestinal ulcers, etc.), liver diseases kidney diseases (nephritis, renal failure, etc.), eye diseases (cataracts, retinal diseases, etc.), skin diseases (dermatitis, photosensitivity) Others Diabetes and immune diseases (AIDS, autoimmune diseases, etc.) have been proven to have excellent effects.

  In particular, as life-related diseases and biological effects, such as diabetes, glucosidase, aldose lectitase, insulin effects are excellent, it has the effect of preventing cell damage (free radicals), and prevents skin aging. prevent. It also helps to prevent the spread of lipid peroxides and neutral fats by adjusting the physical condition such as fatigue caused by activated carbon.

  It also prevents the expansion of unsaturated fatty acids, cleans blood vessels, suppresses the increase of cholesterol and prevents hypertension, thereby preventing myocardial infarction, angina pectoris, cerebral infarction, intracerebral hemorrhage, and preventing anti-ulcer.

  In addition, the effects of the present invention prevent hay fever and atopic symptoms that occur in the lifestyles of the topic these days, and prevent fat saturation, constipation, antirheumatic, antiviral, and swelling. Also effective for general detoxification, while preventing various allergies, relieving stress and relieving sorrow, relieving nerve fatigue, suppressing anger, preventing encephalopathy and mold, and originating from electronic parts such as personal computers It has also been reported to suppress electromagnetic waves that cause adverse effects on the body.

  And so-called blur is prevented, and since the bactericidal effect is great, diarrhea is prevented, insomnia is cured, and coldness is prevented. Furthermore, by drinking the antioxidant of the present invention (nanocolloid water), I caught a cold but did not get fever, and I was cured the next day with sneezing and coughing. The athlete's foot was cured. Shortness of breath was cured. I was so tired of my eyes and clear. My gray hair turned black. Such reports have been made.

Field of application: Use of the invention

  Therefore, the antioxidant application field of the present invention may be used as a pharmaceutical mixed with water or the like, and may be used as a powder tablet or a softening agent such as a PH regulator.

  Moreover, even if it mixes with what is called a drink as a health drink, a soft drink, it may be used for various health foods as a fragrance | flavor of various foodstuffs of humans or livestock, and a pet, and an additive for formulation containing a coloring agent. . This is also optimal as an improved fertilizer for the soil for growing various plants.

  Furthermore, various health goods are now on sale. For example, it may be welded, affixed or embedded in a decorative item such as a bracelet, a ring, a necklace, an earring, or a pierced earring that directly touches the body. Furthermore, it is also effective to blend the nanoceramic fine particles and / or nanocolloid water of the present invention into a cloth used for clothes or the like.

  Subsequently, the occurrence of various germs and fungi has become a hot topic recently, but since the antioxidant of the present invention has an antibacterial action, it can be applied to inks of printed matter including various credit cards, or applied onto the printed matter. It has been reported that this method has an excellent antibacterial effect.

  In addition, the nano-ceramic fine particles and / or nano-colloidal water of the present invention was most suitable for mixing with various cosmetics and bathing agents, and particularly white (skin). It has been reported.

  In addition, it is possible to prevent such occurrences in applications where resin bottles, beer cans (bottles), window glass including the front of automobiles, mirrors, room walls, furniture, and so-called condensation are generated. In addition, it has the effect of preventing adverse effects such as electromagnetic waves emitted from personal computers and the like.

  Thus, nano-ceramic fine particles composed of silicon carbide and its unreacted materials (silicon dioxide, silicon monoxide, metal carbide, etc.) used in the present invention, more specifically, silicon-integrated biomass (plants having a silica (SiO2) component). Compounding raw materials of carbide powder, silicon-integrated biomass carbide powder and silica powder such as quartz or silica-based silica powder, and carbon powder such as graphite and amorphous carbon and quartz Silica powder or a raw material blended with silica and other powders containing silica as a main component.

  Another feature of the present invention is to irradiate a high frequency electromagnetic wave having a frequency of 2 GHz or more using a high frequency electromagnetic wave irradiation device using a high frequency electromagnetic wave absorption characteristic of carbon or carbide to solve the problem of low energy cost. One example is to synthesize silicon carbide into nanoceramic particles.

  This apparatus or means can perform the synthesis reaction in a very short time using the raw material itself as a heating element. That is, this manufacturing apparatus surrounds the raw material with a high fireproof and high heat insulating material, thereby minimizing the diffusion of thermal energy necessary for the synthesis reaction to the surroundings, and by irradiating a high frequency electromagnetic wave having a frequency of 2 GHz or more, Since thermal energy is supplied from the inside of the raw material until the synthesis reaction is completed, the nanoceramic fine particles can be produced in a relatively short time.

  In addition, the present invention can use silicon-integrated biomass carbide as described above as a main raw material, but rice husk is inexpensive as an agricultural waste material, and rice husk is accumulated by the introduction of a recent integrated rice milling method. It is a promising raw material because it can be easily used industrially.

  Thus, the main compositional characteristic of rice husk carbide is that 18% silica and 80% carbon are very uniformly dispersed. In addition, the silica component in the silicon-accumulated biomass exists in the form of silicate gel and is amorphous, so it is more reactive than the silica component in the mineral, leading to a reduction in energy costs.

  Describing the reaction process according to the present invention, the main components of the raw material are silica and carbon (C). By irradiating this raw material with a high frequency electromagnetic wave of 2 GHz or more, the electromagnetic wave energy is absorbed by the raw material and becomes thermal energy. A part of the carbon in the raw material is combusted by this heat, the temperature rises, and oxygen in the air is consumed by the combustion of the carbon to form a reducing atmosphere. By raising the temperature in the reducing atmosphere, a part of the carbon in the raw material burns away from the silica in the raw material, and the silicon from which the oxygen has been removed is combined with carbon to form silicon carbide. Thus, the carbon in the raw material has a portion that becomes a raw material of silicon carbide and a portion that becomes a fuel, and can promote the reaction very efficiently.

  As for the optimal amount of silica and carbon as raw materials, the stoichiometric ratio of the portion contributing to the reaction of raw materials (SiO2 + 3C → SiC + 2CO) is 1 mol of silica: 3 mol of carbon, It is only necessary to add carbon for reaction with oxygen, and the optimum blending ratio can be easily obtained by calculation and experiment.

  Here, a specific example of the purification of the nanoceramic fine particles and / or nanocolloid water of the present invention will be described.

  That is, as an example, in order to produce nano ceramic fine particles and / or nano colloidal water used as an antioxidant, commercially available rice husk carbide (rice husk husk charcoal) is crushed to 40 to 60 μm as a raw material. About 10 g of the raw material is surrounded by a high fireproof and high heat insulating material, and is placed in the high frequency electromagnetic wave irradiation apparatus with 2.5 GHz and output of 500 W and irradiated for about 20 minutes.

  In this process, silicon carbide (CIS) is synthesized, and as the reaction is completed, the electromagnetic wave absorption characteristics are lowered and the temperature is lowered. This is recovered and purified using nano-ceramic fine particles of green silicon carbide. The liquid could be manufactured.

  The anti-oxidant of the present invention nanoceramic fine particles and nocolloid water, which has been made as described above, shows how excellent its antioxidant ability against hydrogen peroxide is, for example, the anti-oxidation of colloidal water in liver homogenate solution Rat liver homogenate was used as a test (which will be described in detail in the following experiment and results section). This is because 0.1 ml of the above colloidal water reference solution was added to 0.5 ml of rat liver homogenate solution prepared according to a conventional method and incubated at 37 ° C. for 10 minutes.

  Thereafter, 0.2 ml of hydrogen peroxide (final concentration 6 mM) and 0.2 ml of iron sulfate (final concentration 6 mM) were added, stirred, and incubated at 37 ° C. for 20 minutes. For the control group, the same amount of ultrapure water and vitamin E (25 μg / ml) were used.

  Thus, rice husk carbide is good as the main raw material for silicon carbide synthesis, and as another example, it was heated to 1400 ° C. or higher in a reducing atmosphere. The reaction formula is SiO2 + 3C → SIC + 2CO. The synthesized silicon carbide is green silicon carbide and is distinguished from general black carbide carbide. The particle size of silicon carbide is about 0.2 to 0.3 μm. A whisker (acicular single crystal) of about 10 μm was also observed. The feature is that general silicon carbide does not show magnetism in a semiconductor, but has KΩ level conductivity and shows magnetism.

  Next, in the examples of the present invention, the nanoceramic fine particles are dissolved in water for purification of colloidal water, and the water obtained by filtration is referred to as nano (silicon carbide) colloidal water. This nanocolloid water had a surface active action and was amphiphilic (hydrophilic and lipophilic), and the hydrogen ion concentration showed a weak acidity of about PH 6.0. The main source cords detected from the colloidal water are 36 types such as Si, Fe, K, Ca, S, Mg, Na, and P. The zeta potential (surface charge) was minus 18.5 mV on average.

The amount of lipid peroxide produced from this reaction was quantified in accordance with the method of Uchiyama et al. After adding TBA (2-tionbarbituric aid) reagent, and the antioxidant capacity expressed by nmo1 MDA / Mg protein was determined as MDA production. Inhibition rate is shown. The MDA production inhibition rate (%) was calculated by the following equation.

(Liquid peroxide production by ultra pure water)-(Lipid peroxide production by colloidal water or vitamin E) ÷ (Lipid peroxide production by ultra pure water) x 100

  As a result, the inhibition rate of MDA production by colloidal water was 58.5%, and vitamin E was 64.8%. Production of lipid peroxidation by this experiment was found to be inhibited by antioxidants, catalase, and glutathione peroxidase (GSH-Px), but not by superoxide dismutase (SOD).

  Further, the colloidal water of the present invention did not show the action of increasing the activity of catalase and glutathione peroxidase. Therefore, it is considered that the colloidal water has a radical scavenger action like vitamin E.

Subsequently, this will be described later in detail as an antioxidant test of colloidal water in rat liver microsomes having antioxidant ability according to the present invention. In this case, 0.2 mm of NADPH is added to the lipid peroxidation reaction by the enzyme. In addition, the lipid peroxidation reaction with ascorbic acid was initiated by adding 0.1 mm of ascorbic acid and incubated at 37 ° C. for 30 minutes. The amount of lipid peroxide produced by this reaction was quantified according to the method of Uchiyama et al. After adding a TBA (2-ionbarbituric aid) reagent, and expressed as n mo1 MDA / Mg protein. Antioxidant activity showed a strong control action with a high MDA production inhibition rate. In addition, MDA production | generation suppression rate (%) was computed by the following formula.

(Amount of lipid peroxide produced by ultrapure water) 1 (Amount of lipid peroxide produced by colloidal water) / (Amount of lipid peroxide produced by ultrapure water) × 100

  As a result, the MDA inhibition rate of colloidal water was 64.9% for NADPH-dependent lipid peroxidation in rat liver microsomes. Also. Induced by alcorbic acid and iron. The MDA inhibition rate by colloidal water for the lipid peroxidation reaction was 54.7%.

  Next, when the magnetic properties of the nanoceramic particles obtained in this way were examined, they showed ferromagnetism. The magnetic characteristic pattern is as shown in FIG. The results of fluorescent X-ray analysis of the nanoceramic fine particles are shown in Table 1. The colloidal water containing the nanoceramic fine particles is prepared by dissolving 10 g of nanoceramic fine particles in 1 liter of water and filtering it with a paper filter after precipitation. The zeta potential of the nanocolloid water reference solution is shown in FIG.

  Of course, the nanoceramic fine particles and / or nanocolloid water of the present invention are not limited to the above-mentioned application form of the antioxidant, and can be used in vivo for various radicals. Examples of radicals that can be eliminated by the antioxidant of the present invention include, but are not limited to, active oxygen such as superoxide anion, hydroxy radical, hydroperoxy radical, lipid peroxy radical, lipid alkoxyl radical, and the like. Never happen. Active oxygen is a suitable erasure target for the antioxidant of one embodiment of the present invention, and particularly preferred erasure target is hydrogen peroxide (H 2 O 2).

  Thus, when this antioxidant is used as a medicine, the nanoceramic fine particles, which are active ingredients, are prepared as pharmaceutical compositions in an appropriate form using additives for preparations such as an adhesive, a softening material, and a solvent. It can be administered parenterally through administration routes such as subcutaneous.

  The nanocolloid water containing nanoceramic fine particles can be administered orally or parenterally. Parenteral administration includes administration routes such as subcutaneous, respiratory tract and rectal. Although colloidal water can be administered alone as a pharmaceutical, it may be administered as a pharmaceutical composition in an appropriate form using a pharmaceutical additive together with colloidal water. Examples of medical compositions suitable for oral administration include syrups.

  Examples of formulations suitable for parenteral administration include inhalants, sprays, suppositories, transdermal absorbents, transmucosal absorbents and the like. In addition to such a pharmaceutical composition, nano colloidal water may be blended as a medicine into drinks, health drinks, soft drinks, health foods, and the like.

  Furthermore, examples of the additive for preparation include a fragrance, a colorant, a pH adjusting agent, a softening agent, and a fragrance, and these two or more kinds may be used in combination.

  Further, it has been described above that the radical to be captured is one or more selected from active oxygen such as superoxide, hydrogen peroxide, hiddlexyl radical, and singlet oxygen. Diseases involving this active oxygen include, for example, the brain, neurological diseases (Alzheimer's disease, Parkinson's disease, cerebrovascular disorder, etc.), pulmonary diseases (pulmonary oxygen poisoning, respiratory prone disease group, airway hypersensitivity, etc.) Blood-reperfusion injury, myocardial infarction, vascular disease (arteriosclerosis, etc.), gastrointestinal mucosal disorder (gastrointestinal ulcer, etc.), liver disease, kidney disease (nephritis, renal failure, etc.), eye disease (cataract, retinal disease, etc.) ), Skin diseases (dermatitis, photosensitivity, etc.), diabetes diseases, immune diseases (AIDS, autoimmune diseases, etc.), and cancer. It is also possible to use this medicine as an antiaging agent.

  In this case, the dose and frequency of administration of the drug are not particularly limited, and depend on various conditions such as the type of disease to be prevented and / or treated, the route of administration, the age and weight of the patient, symptoms, and the severity of the disease. Can be selected as appropriate. In addition, this medicine can be used in combination with an active ingredient of other medicine.

  Further, by blending the antioxidant in this embodiment into food or cosmetics, it is possible to prevent quality deterioration of the food or cosmetics due to radicals such as active oxygen. In addition, for cosmetics, since the protection and preservation of the skin can be expected by suppressing or eliminating the generation of radicals due to external factors such as ultraviolet rays, the nanoceramic fine particles can be used alone with colloidal water as a bath agent. Moreover, you may use it in combination with a fragrance | flavor, a moisturizer, etc.

  Subsequently, the pharmacological properties introduced about the pharmacological action of colloidal water of silicon carbide are as follows, but as described above, the pharmacological properties are not limited to this range.

(1) Inhibition of inflammation (rat test) 20% inhibition rate
(2) Carcinogenic promoter (TPA) inhibitory action a Inhibition of HeLe vesicular phospholipid synthesis enhancement inhibition rate 69%
b 70% inhibition / inhibition of virus (EBV) activity in virus-infected human lymphocytes

(3) The inhibitory effect of diabetic complications is related to diabetic complications (eg cataract). Confirmed the activity inhibitory effect of aldose reductase (AR). ... Inhibition rate 79% (average of two experimental values)
The above inhibition rate is a nanocolloid water stock solution, and in 1/10 dilution water, the inhibition rate is reduced to 40 to 50%. Of course, a higher effect can be expected by increasing the concentration of the stock solution. is there.

(4) Against tumor cells. DNA synthesis inhibitory action
a HGC (Human gastric cancer lees) ... 90% inhibition rate
b HLC (Human Lung Cancer Fistula) ··· Inhibitory Graduation 80%
c U937 (human monocytic leukemia cells) ... 75% inhibition rate

(5) The weight-reducing effect of Sarcoma 180 solid tumor in mice is
-Free intake of silicon carbide colloidal water for 7 days. 41.2% of the tumor weight has disappeared.
-In the case of anticancer drug (FT-207) (500 mg / kg / day) administered for 3 days, 77.4% decreased.

(6) Rat liver homogenate solution. Confirmation of the antioxidant action of the silicon carbide colloidal water confirmed that the silicon carbide colloidal water suppressed an increase in the amount of lipid peroxide. It was found to have a radical scavenger (scavenger) action similar to vitamin E.
・ Inhibition rate of silicon carbide water 58.5%
・ Vitamin E (25μg / mg) Inhibition rate 64.8%
In addition, it confirmed that the substance in nano colloid water was the same as that contained in tap water, and there was no side effect.

Furthermore, if the supplementary explanation about the pharmacological action of the present invention and nano colloidal water is given,
1) Inhibition of inflammation As an acute inflammation model, colloidal water whose volume inhibition rate of paw swelling was measured by carrageenin rat foot edema method showed inhibition of 20%.
2) Carcinogenic promoter (TPA) inhibitory action showed a 69% inhibition rate in HeLa cells in terms of the inhibitory effect on carcinogen (TPA) cell phospholipid synthesis enhancement (high peak). Similarly, EBV latently infected human lymphoblast cells inhibited viral (EBV) activation by 70%.

  3) The inhibitory action of diabetic complications is related to therapeutic drugs for diabetic complications. Inhibition of the activity of the substance aldose receptor (AR) was confirmed. Inhibition rate 79%. Expectations for prevention and treatment of diabetic complications such as cataracts.

  4) To tumor cells. The growth inhibitory effect was confirmed in the DNA synthesis inhibitory effect in cultured human tumor cells. The inhibitory effect was 90% for human gastric cancer cells (HGC), 80% for human lung cancer cells (HLC), and 75% for human monocyte leukemia cells (U937).

5) The effect on solid tumors in mice was obtained by freely ingesting nanocolloid water for 7 days by transplanting tumor cells into mice, developing cancer, and examining changes in wet weight of solid tumors.
As a result, the inhibition rate (weight reduction rate) of the mouse was 44.2%, and it was confirmed that the tumor was reduced. Similarly, when the anticancer drug was administered (500 mg / kg / day) for 3 days, the inhibition rate was 77.4%. As a result, the anticancer activity of nanocolloid water was confirmed.

6) The antioxidant effect of nano colloidal water was found to increase markedly when lipid peroxide and iron sulfate were added to rat liver homogenate and incubated (incubated). However, in the liver homogenate solution to which 0.1 ml of colloidal water was added, suppression of lipid peroxide weight was confirmed. From this, it is thought that it has a vitamin E-like radical scavenger (scavenger) action.
The water inhibition rate was 58.5%, and vitamin E (25 μg / ml) was 64.8%.

7) Antioxidant action of nano colloidal water was obtained by adding 0.2 ml of 3 mM ADP, 0.2 ml of ferric chloride and colloidal water to 0.5 ml of rat liver microsome (0.5 ml prot./ml) solution at 37 ° C. Incubated for minutes.
Thereafter, 0.2 mm NADPH was added to the lipid peroxidation reaction by the enzyme, and as a result, 0.1 ml of ascorbic acid was added to the lipid peroxidation reaction by alcorbic acid in which the amount of lipid peroxide was suppressed by 64.9%. The amount of lipid peroxide was suppressed by 54.7%.

8) Nanocolloidal water affecting the intestinal disaccharide-degrading enzyme activity: rat small intestinal imprint membrane cell solution 0.3ml water 0.2ml, control group 0.5ml ultrapure water disaccharide 0.5ml maltose, saccharose, Each lactose solution was added and incubated. Water markedly suppressed the increase in the amount of glycolose with respect to the disaccharide-degrading enzyme activity.
Moreover, it was accompanied by the inhibition rate of maltase: 61.5%, lactase: 56.1%, saccharase: 25.0%. As a result, it can be explained that drinking this nanocolloid water immediately before a meal suppresses an increase in blood glucose level (glycose level) after a meal of a diabetic patient.

In addition, the synthesis material of the above-mentioned nano ceramic fine particle nano colloidal water of the present invention is generally made of glass as long as there is silicon dioxide (silica) (Si2O3), iron (catalyst) carbon material and silica in addition to rice husk charcoal as described above. Charcoal can also be synthesized and is within the scope of the embodiments of the present invention.
Moreover, as reaction conditions, 1200 to 1500 degreeC (reducing atmosphere) CO2 partial pressure control is required.

  Silicon carbide synthesized as a feature of the above-described nanoceramic fine particles of the present invention and nanocolloidal water (silicon carbide: S1C) is said to have higher purity than black silicon carbide. The black impurity is green silicon carbide which is aluminum, iron or the like.

  Next, the characteristics of nanocolloid water will be described. When nanoceramic fine particles and unreacted substances are dissolved in water in the range of 1 to 5 Wt%, filtered with paper and applied to the skin, viscosity is felt.

  Also, the taste as a drink has changed deeply and deliciously. Furthermore, due to the effect of the surface activity, it was sprayed on glass such as glasses, and when it was wiped off, the dirt was well removed.

  Note that fresh water silicon of nano colloidal water has a hydrogen ion concentration (PH) of about 6.0 (1 Wt% to 0.1 W t% was also the same), and the electric conductivity (EC) of sample pure water was about 0.00. For 93 μS / cm (micro Siemens percentimeter), the eluate was 2.5 to 1.5 μS / cm, indicating that it was not very soluble.

  Furthermore, when observing nano colloidal water, the colloidal state was observed using a laser pointer, and as a result, the Tyndall phenomenon (red laser) was confirmed. Moreover, the nano colloid particle was 1-500 nm, and the hydrogen atom was 0.1 nm.

  Next, the basic anti-caries effect against mutans streptococci (caries causing caries) could be confirmed in the nanoceramic fine particles and nanocolloid water of the present invention. Moreover, the glucosyltransferase (Gtase) inhibitory effect which is concerned in colonization of bacteria was also verified.

Furthermore, the nanoceramic fine particles and nanocolloid water of the present invention are optimal for applications in the field of pursuing “whitening” effects such as cosmetics. That is, the key point of whitening is the melanin production inhibitory effect, and this action also makes it possible to prevent spots.
Therefore, focusing on the antioxidant action of the nanocolloid fine particles and / or nanocolloid water of the present invention, the melanin production inhibitory effect that causes pigmentation after inflammation and senile pigment spots was verified using tyrosinase activity as an index. is there.

  Subsequently, the nanoceramic fine particles of the present invention and the nanocolloid water of the present invention have an antibacterial action, and at least silica particles can be obtained by adding the nanoceramic fine particles of the present invention to an accessory material or a part thereof such as a ring or a bracelet in contact with the body. (Silica) and black silica (graphite silica) can now exhibit more than the unique effects.

In particular, since the nanoceramic fine particles and nanocolloid water of the present invention have an antibacterial action, they exhibit excellent effects for growing almost any plant as a fertilizer, for example.
Additives for preparations, fragrances, various colorants, pH regulators, softeners, fragrances, deodorants, bathing agents, fungicides, other bathing agents, detergents, gloss accelerators, and various bottles, mirrors and glass It is also effective as an anti-condensation agent on the surface of the appliance.

Experimental examples and examples

(Example 1 Proof that there is no toxic effect)

  Thus, first, an acute toxicity test of the nanocodoid water of the present invention (see Table 2) was attempted.

1. In the test substance test, the nanocolloid water of the present invention was ingested freely for one month. In addition, as an untreated control group, distilled water was ingested freely for one month.

2. Animals Used and Breeding Conditions Four-week-old SD strain (Crj: CD) rats were purchased from Japan Charles River Co., Ltd. and subjected to quarantine acclimation for 12 to 13 days, and then healthy animals were subjected to the test. Animals are housed in a stainless steel hanger cage in a barrier system breeding room set at a temperature of 24 ± 2 ° C, humidity of 55 ± 10%, illumination for 12 hours (7 am to 7 pm) and 15 to 20 awakenings / hour. Two animals were housed and reared. As for the materials, solid feed (CE-2 manufactured by CLEA Japan, Inc.) was freely ingested. Rats used 8 animals in each group.

3. Observation and examination items 1) General condition and body weight The general condition of each animal and the presence or absence of fat were observed twice daily in the morning and afternoon in the laboratory. The body weight was measured at a frequency of twice a week for 2 weeks from the test start date and once a week from the 3rd week to the end of the test.

2) Blood biochemical test Blood collected at the end of the test was centrifuged at 3000 xg for 10 minutes to obtain serum. The obtained serum contains total protein (lowry modified method), total viruribine (alkaline method), GOT (AST) activity (Kaemen method), GPT (ALT) activity (Karmen method), alkaline phosphatase activity (ALP, p -Nitrophenyl phosphate substrate method), γ-glutamyl transpeptidase activity (γ-GPT, γ-glutamine-p-nitroanilide substrate method), total cholesterol amount (COD-DAOS method), triglyceride amount (GPO-DAOS method) ), The amount of phospholipid (enzyme method), and the amount of glucose (glucose oxidase method) were measured according to conventional methods.

3) Sectional examination and organ weight measurement At the end of the examination period, blood was collected from the rats and then dissected to perform macroscopic observation of various organs and tissues. After the mass examination, organ weights of the brain, heart, lung (including bronchi), liver, pancreas, kidney, adrenal gland and testis were measured.

4) Statistical processing The experimental results obtained were expressed as mean ± standard error, and Dunnett's method was used for the significance test.

Considering this result, there were no deaths in rats due to free intake of the nanocolloidal water of the present invention for one month.
In addition, changes in organ weight and appearance due to administration of the nanocolloid water of the present invention were not observed as compared with control rats (Table 3). In the blood biochemical test (Table 4), the glucose level tended to be slightly lowered by administration of the nanocolloid water of the present invention, but no significant change was observed in other test items compared to the control. It was.

  From the above, it was proved that there is no toxicological effect of the nanocolloid water of the present invention under the test conditions.

(Example 2 Proof of no side effects in DNA synthesis)
Subsequently, for the growth inhibitory effect on the various tumor cells of the nanocolloid water of the present invention, the stock solution and dilution solution of the present invention nanocolloid water (diluted stock solution to 1/10, 1/100, 1/1000 with ultrapure water) First, the effect on DNA synthesis in cultured cells was examined.

Ultrapure water was used as a control for this, and after 48 hours of culturing with 10% (V / V) FCS-containing RPMI-1640, the uptake of 3H-thymidine was measured with a liquid scintillation counter.

In this case, in preparing a cultured human tumor cell suspension,
HGC (human gastric cancer cells: 2 × 10 5 cellS / ml)
HLC (human lung cancer cells: 1 × 10 6 cells / ml)
U937 (human monocytic leukemia cells: 1 × 10 6 cells / ml)
These three types of cells were adjusted to an appropriate concentration with RPMI-1640 with 10% (V / V) FCS and used as a cell suspension.

Consideration of this experimental result showed that the remarkable effect of DNA synthesis inhibition with respect to various tumor cells of the nano colloidal water of the present invention was obtained.
That is, as a result of examining the influence of each nanocolloidal water of the present invention on DNA synthesis, a difference was observed in the DNA synthesis inhibitory action depending on the dilution ratio of the nanocolloidal water of the present invention in all cultured tumor cells. The DNA synthesis inhibitory effect of the nanocolloid water of the present invention was observed to be stronger in cancer cells than in leukemia cells. (Figure 4).

As described above, from this experiment, the nanocolloid water of the present invention was found to have an inhibitory effect on the DNA synthesis of tumor cells. Since the nano water of the present invention has almost no side effects and can be used for a long period of time, it was considered that the use value as an anticancer agent was high.

(Example 3)
This example is a proof experiment in which an inhibitory action was observed in the production of PGE2 in the mucosa of the large intestine.

  Next, an experiment on the influence of the nanocolloid water of the present invention on the PGE2 production of the colonic mucosa was attempted. The purpose is that prostadarandin E2 produced in vivo is considered to be involved in the progression of the carcinogenic process, and it has been clarified that substances that suppress their production show cancer preventive action. is there.

In the study in the present invention, it was confirmed that the nanocolloid water of the present invention has an action of suppressing the carcinogenic process using a rat colon cancer model. Therefore, in the present invention, the effect of the nanocolloid water of the present invention on the production of PGE2 has been investigated for the purpose of elucidating the mechanism of action.

PGE2 is one of prostaglandins generated from arachidonic acid by cyclooxygenase (COX), and is considered to be involved in tumor cell proliferation, apoptosis inhibition, angiogenesis and the like in carcinogenesis.

As an experimental method, Wistar male rats (n = 6) aged 4 weeks were injected with azoxymethane (AOM) 15 mg / kg subcutaneously three times in total at 1, 2, and 3 weeks. used. The nano colloidal water of the present invention was given from one week before AOM administration and was ingested freely during the experimental period. The resulting PGE2 concentration was measured using a PGE2 measurement kit (Prostaglandin E2 Express EIA Kit).

Thus, the results are shown in the influence of the nanocolloid water of the present invention on the production of PGE2 as shown in FIG. This indicates that the nanocolloid water of the present invention has an inhibitory effect on PGE2 production, and that the inhibition of the formation of precancerous colorectal disease in the promotion phase by the nanocolloid water of the present invention is related to the inhibition of PGE2 production on the colonic mucosa. It has been done.

Example 4
This example is proof that the inhibitory action of glucose was observed in the activity of degrading enzyme of the small intestine.

Subsequently, the effect of silicon carbide water on the intestinal disaccharide-degrading enzyme activity was tested. This was performed by adding 0.3 ml of rat small intestine brush border membrane cell solution prepared according to a conventional method to the test water (silicon carbide water: undiluted solution). ) 0.2 ml, and the same amount of ultrapure water was added to the control group and preincubated at 37 ° C. for 5 minutes. Thereafter, 0.5 ml maltose, saccharose and lactose solution (final concentration: 42 mm) were added, and the reaction was carried out by incubating at 37 ° C. for 20 or 30 minutes.

In addition, the measurement of the disaccharide-degrading fermentation activity is quantitative, and mg / min / Mg prot. Table 5 shows.
This result and discussion showed that a significant increase in disaccharide-degrading enzyme activity was observed when three types of disaccharides were added to rat small intestine brush border membrane cell fluid and incubated at 37 ° C.

On the other hand, in the rat small intestine brush border membrane cell solution to which silicon carbide water was added, the disaccharide-degrading enzyme activity was reduced and the increase in the amount of glucose was remarkably suppressed. Moreover, the intensity of suppression of disaccharide-degrading enzyme activity in water was in the order of maltase> lactase> saccharase. Therefore, it was shown that the nanocolloid water of the present invention suppresses the disaccharide-degrading enzyme activity. (See Table 5)

From the above, it was shown that a significant increase in blood glucose level (glucose level) seen after meals of diabetic patients can be suppressed by drinking an appropriate amount of the nanocolloid water stock solution of the present invention immediately before a meal.

We also experimented with the effect of water on insulin secretion after ingestion of toose. This is because disaccharide-degrading enzyme inhibitors are considered to be useful therapeutic agents for the purpose of alleviating postprandial hyperglycemia in diabetic patients. It is conceivable that the diatomase-degrading enzyme inhibitor decreases postprandial insulin secretion with a postprandial blood glucose level suppressing action.
In this study, we investigated the relationship between water disaccharide-degrading enzyme inhibitory effect and insulin secretion using rats.

In addition, the nano ceramic fine particles of the present invention and the nano colloidal water of the present invention are related to the life of humans and animals, for example, mixed with printed coatings and inks to have an antibacterial action, Used for bathing agents, various health goods and decorations, for plant growth and its fertilizer, food, feed, additives, anti-tacking, fragrances for preparation additives, coloring agents, pH control There have been many proofs of no adverse effects on human animals or plant growth as agents, various softeners, fragrances, and anti-condensation agents, which will be explained in order in the following examples. .

Example 5 Carcinogenicity Motor Inhibition Proof Experiment 1
First, carcinogenic promoter inhibitory activity and aldose reductase inhibitory activity (-in vitro) of the nanocolloid water of the present invention were examined. The purpose of this study was to investigate the inhibitory activity of the nanocolloid water (or diluted solution thereof) of the present invention on the oncogenic promoter, and the aldose reductase inhibitory action related to the therapeutic agent for the prevention of diabetic complications (such as diabetic cataract). is there.

As an experimental method, the control group (control group) using the nano colloidal water of the present invention: undiluted solution and its diluted solution (1/10, 1/100; diluted with ultrapure water) had ultrapure water used for dilution. It was used. Carcinogenic promoter: TPA (12-O-tetradecanoylphorbol-13-acetate) aldose reductase (derived from human muscle cells) or higher was used, and all other reagents were tested using special grades. (See Table 6)

As a result, the enhancement of phospholipid synthesis in HeLa cells (MEM liquid medium) by TPA (50 nM) is suppressed by the method using the inhibitory effect on the increase in cellular phospholipid metabolism by TPA. Efficacy of each nano-colloidal water of the present invention (stock solution, diluted to 1/10, 1/100. Nano-water of the present invention is added to 0.15 ml cells). The cells were cultured for 48 hours and examined by the method of Nishino et al. In addition, the inhibition rate of incorporation into 32 pi phospholipid was expressed in%, and the measurement was performed with a liquid scintillation counter.
This measured value is the same as the average of the experimental values of Tables 6 to 8 from the two experimental values.

(Example 6: Carcinogenicity poromoter suppression proof experiment 2)
Experiment 2 is TPA. A method using the efficacy of inhibiting the activation of Epstein-Barr virus (EBV) as an index was used. This is because 0.15 ml of TPA (32 pM) and each nano water of the present invention (diluted solution, 1/10, 1/100 dilution) was added to EBV latently infected human lymphoblastoid cell line Raji cells and cultured for 48 hours EBV-ear1 antigen (EBV-EA) was stained by the indirect fluorescent antibody method, the expression rate of positive cells was taken as 100, the expression rate (%) of EVB-EA relative to the positive control was calculated, and this value was used as the inhibition rate. Converted. (See Table 7)

(Example 7 Carcinogenic Promoter Inhibition Proof Experiment 3)
Experiment 3 examined the aldose reductase inhibitory activity as shown in Table 6. Each nanocolloid water of the present invention (diluted solution, 1/10, 1/100 diluted solution) 0.5 ml, 3% of aldose reductase 0.1 ml of 100 mM dl-glyceraldehyde solution was added to 0.1 ml of phosphoric acid buffer solution, and the change in absorbance at 340 nm was measured for 180 seconds to calculate the inhibition rate (%). The measured value is shown as an average value of two experimental values.

This result and discussion showed that the inhibitory activity with a high inhibition rate of 69% was recognized in the stock solution, as shown in the method using the inhibitory effect as an index for the enhancement of cellular phospholipid metabolism by TPA as an inhibitor of carcinogenic promoter. However, high activity was not expected in the nanocolloid water of the present invention diluted to 1/10 and 1/100.

Table 7 shows the results obtained by a method using as an index the efficacy of inhibiting the activation of Epstein-Barr virus (EBV) by TPA. Although high inhibitory activity with an inhibition rate of 70% was observed in the stock solution, high inhibitory activity was not expected in the nanocolloid water of the present invention diluted to 1/10.

From the above results, a strong carcinogenic promoter inhibitory action was observed in the stock solution of the nanocolloid water of the present invention, but no activity suppression was observed in the diluted nanocolloid water of the present invention.
However, it was clear that the nanocolloid water of the present invention as a stock solution has a strong tumor promoter suppressing action.

Furthermore, aldose reductase inhibitory activity was examined. As a result, as shown in Table 8, a high suppression of AR activity with an inhibition rate of 79% was observed in the stock solution. However, the activity of the nanocolloid water of the present invention diluted to 1/10 and 1/100 appeared to be insufficient.

From the above, it was proved that the stock solution of nano-colloidal water of the present invention has a high AR activity inhibitory effect, and thus has a remarkable effect in preventing cataracts of diabetic complications described later. .

(Example 8 Preventive effect on colorectal carcinogenesis)
Next, experimental examples of the preventive effect of azochimesitane-induced rat colon carcinogenesis by administration of the nanocolloid water of the present invention will be described.
The purpose of this experiment was to use a rat colon colorectal carcinogenesis model to reduce the risk of carcinogenesis by ingesting the nanocolloid water of the present invention. : ACF) The number of occurrences, ornithine decarboxylase activity (ODC), which is a biochemical index of carcinogenesis promotion, was measured and examined.

As an experimental method, Wistar male rats aged 4 weeks (n = 6) were injected subcutaneously with azoxymethane (AOM) 15 mg / kg three times in total at 1, 2, and 3 weeks, and at the 8th week, colonic mucosa The number of pre-cancerous lesions and the number of atypical crypt foci that had occurred were calculated (the colon was stained with 0.2% methylene blue staining solution and observed under a stereomicroscope (x40)).
Furthermore, ornithine decarboxylase activity (ODC) in the colonic mucosa, which is a biochemical index of carcinogenesis promotion, was measured according to the method of Garewal et al. The nano colloidal water of the present invention was given one week before AOM administration and was freely taken during the experiment period.

As a result and discussion, the body weight of the rats was 257 ± 14 in the control group (g), 234 ± 17 in the AOM county (FIGS. 6 and 7), and 242 ± 15 in the AOM + inventive nanocolloid group. There was no.
Moreover, as a result of examining the ACF number showing the precancerous lesion, the ACF number was 168.5 ± 27.8 in the AOM group, 119.6 ± 19 in the AOM ± inventive nanocolloid group, and 30% Showed a decrease.
In addition, the above-average crypt foci per lesion decreased with administration of the nanocolloidal water of the present invention. (See Figure 8)

Furthermore, regarding the large ACF (size of 4 or more), 29.8 ± 7.4 for the AOM group and 19.2 ± 6.4 for the AOM + inventive nanocolloid group. As for the ODC activity value (FIG. 9), a significant decrease in activity was observed in [AOC + invention nanocolloid group] as compared with the AOC group.

From the above results, it was shown that the nano colloidal water of the present invention suppresses the number of AOM-induced colorectal cancers. Furthermore, since the nanocolloid water of the present invention decreases the ODC activity value, it is considered that the progression to a cancer with a lower degree of differentiation may be suppressed, and it was proved that the suppression at the initiation stage of colon carcinogenesis was strong.

(Example 9: 1 of analgesic and purine tumor suppression proof experiment)
Next, in examining the analgesic effect of the nano colloidal water of the present invention using a mouse, a study was made by a writing reaction with acetic acid.
First of all, when observing the act of writhing by feeling the pain caused by stimulation of acetic acid in the abdominal cavity, this paining reaction was suppressed by administering a certain drug, and pseudo pain was suppressed. And it is evaluated that it has an antinociceptive effect.

As the method,
1) ddy male male mass (7 weeks old; 27-38 g, each group uses 6 animals).
2) Chemical solution: aspirin: 0.1M Tris-HCl buffer (pH 7.8) is used as the solvent.
3) 0.7% acetic acid solution: A physiological saline solution is added to 0.7 ml of acetic acid to make a total volume of 100 ml.
4) Nanocolloid water of the present invention: 0.9 g of sodium chloride was added to 100 ml of the present nanocolloid to make an isotonic solution.

As an experimental method,
1) The mice were divided into 3 groups, the aspirin (300 mg / kg) administration group was subcutaneously administered with the aspirin solution on the back, and the control group was subcutaneously administered with Tris-HCl buffer. The nona colloidal water (0.1 ml / 10 g) administration group was also administered subcutaneously in the same manner.
2) 30 minutes after administration, 0.7% acetic acid solution was intraperitoneally administered at a rate of 0.1 ml / 10 g body weight.
3) The number of writing was started 10 minutes after the administration of acetic acid, and the number of writing during 10 minutes was recorded. (See Figure 10)

  As a result and consideration, the average value of the number of times of each group was shown in FIG. As a result, when the 0.7% acetic acid solution was administered, it was 45.4 ± 3.2 times (N = 6) in the control group. In the administration group of Aspiri 300 mg / kg, the frequency was 10.2 ± 2.4 times. Moreover, in the nano colloid water administration group of this invention, it was 17.4 ± 2.2 times and showed the significant fall compared with the control group.

  From the above, it was shown that aspirin and nona colloidal water have an anti-nociceptive effect in the acetic acid writing method. From this, it was proved that the nanocolloid water of the present invention has an analgesic action against chemical stimulation in the abdominal cavity or an anti-inflammatory action for suppressing inflammation in the abdominal cavity. In this experiment, six mice were used, and the results were the same and the reliability was very high.

(Example 10: 1 of analgesic and pure tumor suppression proof experiment)
A rat experiment in which inflammatory pain was caused on the hind paw was conducted as to how the analgesic and purine suppressing effects of the nanocolloid water of the present invention were superior.

That is, 40% of the nanocolloid water of the present invention was blended in a commercially available ointment base, and 100 mg thereof was applied to examine inflammation and analgesic action.
The inflammatory action is shown in the graph of FIG. Further, the degree of the damage was measured in the graph of FIG. 12, and the vertical axis represents the pain threshold ratio. That is, the change of the threshold value when the threshold value of pain in the hind legs before inflammation is set to 1 is represented.

  Thus, when comparing each of these graphs, there is a clear significant difference in these graphs, which proves that the nanocolloid water of the present invention has an excellent effect on analgesic and purine tumor control. is there.

(Example 11 Anti-inflammatory action proof experiment)
For anti-inflammatory activity of colloidal water, Wistar male rats were used, and the volume of the right hind footpad was measured using Plethysmometer (UNICOM, TK-10) to conduct pre-administration values.

  Thereafter, in FIG. 13, 1.5 ml of the nanocolloid water of the present invention was contained in 1.5 mm × 1.5 mm absorbent cotton and applied to the right hind paw for 4 hours (a in the figure). The other group was replaced with new cotton wool absorbent cotton in 2 hours (b in the figure) and applied for a total of 4 hours. Tap water was used as a control. An edema was induced by administering 0.05 ml of physiological saline containing 1% carrageenan as a flame retardant under the right hind footpad.

As a result and consideration, the edema rate and the inhibition rate were calculated from the difference between the toe volume before the induction and the toe volume before the induction. The edema rate after 3 hours was 80% for tap water, 70% for the nanocolloid water of the present invention for 4 hours, and 60% for 2 hours x 2 times of the nanocolloid water of the present invention. From this, the anti-inflammatory action of the nano colloidal water of the present invention was proved.

Example 12 Scar Suppression Proof Experiment
Subsequently, Table 9 shows an example in which Sarcoma 180 was transplanted as an effect on Sarcoma 180 solid tumor and ascites tumor production. Thus, ascites was collected from the abdominal cavity of a mouse (ICR male), and the number of tumor cells was adjusted to 2 × 10 6 cells / 0.05 ml with physiological saline, and prepared subcutaneously on the back of the mouse for solid tumor testing. did.

Next, 0.05 ml of each tumor cell was injected and transplanted, and the tumor was excised 13 days after the transplantation and the wet weight was measured. The inhibition rate (%) was calculated as follows.

Inhibition rate (%) = (1−B / A) × 100

A represents the average tumor wet weight of the control group, and B represents the average tumor wet weight of the drug and nano-water administration group of the present invention. The nanocolloid water of the present invention was ingested freely for 14 days from the date of transplantation. Further, FT-207 (5-FU derivative), which is a positive control drug, was orally administered in the same manner.

  Subsequently, ascites is collected from the abdominal cavity of a mouse (ICR male) transplanted with Sarcoma 180, and the number of tumor cells is adjusted to 1 × 10 6 cells / 0.05 ml with physiological saline. Prepared intraperitoneally in mice for ascites tumor testing.

In addition, 0.05 ml of tumor cells were injected and transplanted, and ascites was collected from the abdominal cavity of an ascites tumor test mouse 8 days after the transplantation. The tumor cells were separated by centrifugation at 900 × g for 5 minutes, and then the tumor cell volume (PVC) was measured. At this time, the inhibition rate (%) was calculated as follows.

Inhibition rate (%) = (1−B / A) × 100

  In addition, A shows the average PCV of the control group, and B shows the average PCV of the drug and the nanowater administration group of the present invention. The nanocolloid water of the present invention was orally administered for 7 days before transplantation and for 15 days from the date of transplantation to 8 days. In addition, FT-207 (5-FU derivative), which is a positive control drug, was orally administered in the same manner, and a remarkable effect of inhibiting DNA synthesis on various tumor cells of the nano-water of the present invention was obtained.

  That is, as a result of such experiments, the nano water of the present invention showed a tendency to reduce the weight of Sarcoma 180 solid tumor. Moreover, the inhibition rate of the 7th and 14th administration of the stock solution water was 41.2% and 43.6%. The positive control drug FT-207 (500 mg / kg / day) was administered for 3 days to reduce the tumor weight, and the inhibition rate was 77.4%. (See Table 9)

  From the above results, the nanocolloid water of the present invention is the first stage of initiation (initial stage) normal DNA is damaged by some substance, DNA mutation occurs and cells are transformed into cancer cells, promotion ( It has been proved that there is an effect in which normal cells that are in the promotion stage) deviate from normal control and abnormally proliferate, and that the effect is particularly excellent in the early stage .

Example 13 Spontaneous Diabetes Suppression Proof Experiment
Next, an experiment was conducted on the anti-diabetic action of the nanocolloid water of the present invention on spontaneously diabetic rats (GK rats).

  Here, we examined the improvement effect of the nanocolloid water of the present invention on NIDDM using GK rats, a non-obese type non-insulin-dependent diabetes mellitus (NIDDM) model in which impaired glucose tolerance and glucose-stimulated insulin secretion are recognized. went. The method used male GK rats (about 320 g) about 15 weeks of age as an oral carbohydrate tolerance test.

  As controls, rats using Wistar male rats of the same week were divided into four groups: a subject group, a GK group, a GK + inventive nanocolloid water administration group, and a GK + voglibose administration group. The experiment used 4 rats per group.

In the nanocolloid water administration group of the present invention, 2 mL / kg / day of the nanocolloid water of the present invention was continuously administered for 5 days, and the same amount of physiological saline was administered to the control group, the GK group, and the GK + voglibose administration group.
The carbohydrate load test (starch: sucrose: lactose = 6: 3: 1) used rats fasted for 18 hours after the final administration, and the nanocolloid water of the present invention (2 ml / kg) and voglibose 30 minutes before the load test. (0.2 mg / kg) was administered. These groups of rats were orally administered with carbohydrates (2 g / kg) dissolved in raw food, blood was collected from the tail vein over time, and blood glucose levels were measured.

  As a result, as shown in FIG. 14, the blood glucose level of each group 30 minutes after carbohydrate loading was 251 ± 21 mg / dL in the control group. The blood glucose level of the GK + inventive nanocolloid group at that time was found to be about 70 mg / dL lower than that of the GK group. However, the blood glucose level of the GK + nanocolloid group of the present invention was about 30 mg / dL higher than the group administered with voglibose, a disaccharide-degrading enzyme inhibitor.

  Inferring from the above, the nanocolloid water of the present invention has a moderate inhibitory effect compared to a commercially available disaccharide degrading enzyme inhibitor, but proves that the disaccharide degrading enzyme inhibitory effect in the diabetic state is sufficient. It was done.

(Example 14 Experiment for proof of influence of insulin secretion)
Then, it experimented about the influence of this invention nano colloid water on the insulin secretion after ingesting maltose. In this case, the disaccharide-degrading enzyme inhibitor is a useful therapeutic agent for the purpose of alleviating postprandial hyperglycemia in diabetic patients. A disaccharide-degrading enzyme inhibitor is considered to have a possibility that insulin secretion after a meal is reduced with a postprandial blood glucose level suppressing action. Then, the relationship between the disaccharide-degrading enzyme inhibitory effect of this invention nano colloid water and insulin secretion was examined using the rat.

As an experimental method, Wistar male rats (N = 8) weighing about 250 g were fasted for 18 hours and used. Thereafter, the abdomen was opened under anesthesia, the gastric pylorus was ligated, and 0.5 g / kg maltose was injected as a 20% solution from the end of the duodenal mouth. In addition, a maltose solution dissolved in the nanocolloid water of the present invention was prepared and injected in the same manner.
In the control group, only maltose solution was injected. Blood samples were collected from the hepatic portal vein and the inferior vena cava 40, 80, and 120 minutes after maltose solution injection, and glucose and insulin (IRI) levels of portal vein blood and peripheral vein blood were measured. And IRI concentration difference (P-V glucose difference, P-V IRI difference). IRI measurement was performed by the two antibody method.

  As a result, P-V glucose difference showed a decrease of 50% or more in the nanocolloid water group of the present invention after 80 to 120 minutes as compared with the control group. Similarly, the P-V glucose difference was also found to decrease by 50% or more in the nanocolloid water injection group of the present invention after 80 minutes and 120 minutes.

  As a result of this consideration, in this experiment, the product of glucose and insulin concentration in hepatic portal arterial blood and hepatic portal blood flow is the intestinal glucose absorption and insulin secretion. The difference in glucose and insulin concentrations in the peripheral vein was measured.

As a result, it was shown that the nanocolloid water of the present invention also decreased the amount of insulin secretion in proportion to the effect of decreasing the amount of glucose absorbed, as shown in FIGS.
That is, it was shown that nanocolloid water has a disaccharide-degrading enzyme inhibitory action, and further, along with this inhibitory action, the secretion amount of insulin is also reduced.

Example 15 Liver Microsome Inhibition Proof Experiment
Furthermore, the anti-invention nanocolloid water antioxidant test in rat liver microsome was performed. It was prepared according to a conventional method, with 0.5 ml of rat liver microsome (0.5 mg prot./ml) solution, 0.2 ml of 3 mM ADP and 0.2 ml of 0.15 mM ferric chloride, test water (silicon carbide water: stock solution) 0.1 ml of the control group was added with the same amount of ultrapure water and pre-incubated at 37 ° C. for 10 minutes.

  Thereafter, the lipid peroxidation by the enzyme was started by adding 0.2 mM NADPH, and the lipid peroxidation by ascorbic acid was started by adding 0.1 mM ascorbic acid, followed by incubation at 37 ° C. for 30 minutes.

  The amount of lipid peroxide produced by this reaction was quantified according to the method of Uchiyama et al. After adding a TBA (2-thiobarbituric acid) reagent, and tabulated in nmol MDA / mg protein.

As a result and discussion, it was found that the nanocolloid water of the present invention inhibited the lipid peroxide amount by 64.9% against NADPH-dependent lipid peroxidation in rat liver microsomes.
In addition, the nanocolloid water of the present invention was found to suppress the lipid peroxide amount by 54.7% against the lipid peroxidation reaction induced by ascorbic acid and iron.

  From the above results, it was proved as shown in Table 10 that the nanocolloid water (silicon carbide fine particle water) of the present invention has a strong inhibitory action on the lipid peroxidation reaction in rat liver microsomes.

Example 16 Liver Homogenate Inhibition Proof Experiment
Next, the antioxidant test of the nano colloidal water of the present invention in the liver homogenate solution was performed. This was done by adding 0.1 ml of test water (silicon carbide water: stock solution) to 0.5 ml of rat liver homogenate solution prepared according to a conventional method, and adding the same amount of ultrapure water to the control group, followed by preincubation at 37 ° C. for 10 minutes. .

  Thereafter, 0.2 ml of hydrogen peroxide (final concentration: mM) and 0.2 ml of iron sulfate (final concentration: 0.66 mM) were added and the reaction was incubated at 37 ° C. for 20 minutes. The amount of lipid peroxide was quantified according to the method of Uchiyama et al. After adding TBA (2-thiobarbituricacid) reagent, and expressed in nmol MDA / mg protein.

  As a result and discussion, when the hydrogen peroxide and iron sulfate were added to the liver homogenate solution and incubated at 37 ° C., the amount of lipid peroxide was significantly increased. However, the silicon carbide water suppressed the increase in the amount of lipid peroxide in the liver homogenate solution.

  Production of lipid peroxidation by this experiment is suppressed by an antioxidant, catalase, or glutathione peroxidase (GSH-px). Is reported not to be suppressed by SOD. Further, although not shown in the data, silicon carbide water increases catalase and GSH-px activity. There was no effect. (See Table 11)

  From the above results, silicon carbide water is considered to have a vitamin E-like radica1-scavenging action.

(Example 17 caries-causing fungus action proof experiment)
Next, in the present nanocolloid fine particles and / or nanocolloid water of the present invention, a basic anti-caries effect against Streptococcus mutans (caries that cause caries) can be confirmed, and glucosyltransferase involved in colonization of the bacteria. The (Gtase) inhibitory effect could also be verified.

  That is, first, caries-causing bacteria were aerobically cultured using Streptococcus mutans MT8148R and using brain heart infection (BHI) agar medium (DIFCO). Further, the growth inhibitory effect on caries-causing bacteria: Streptococcus mutans MT8148R was inoculated into a BHI liquid medium and aerobically cultured at 37 ° C. for 24 hours to obtain a bacterial solution. Further, 50 μl of a sample solution (blank: sterilized purified water, sample: SIC; 0 (standard) and 10 times <10 times the standard concentration>) and 50 μl of a bacterial solution were added and cultured aerobically at 37 ° C. ( (See Table 12).

  Thus, after culturing for a specified time, the culture solution was diluted and smeared on BHI, and cultured for 48 hours. After that, colony forming units (CFU) / ml at each sample concentration and action time were obtained from the number of colonies, and the Gtas inhibitory effect was observed. After examination, a sample was prepared. After the insoluble insoluble glucan was separated, the absorbance at 490 nm was measured by the phenol sulfuric acid method.

  After inoculating Streptococcus mutans MT8148R in the BHI medium supplemented with the nanocolloid microparticles and / or nanocolloid water of the present invention in order to verify the antibacterial activity of the resulting nanocolloidal microparticles and / or nanocolloidal water of the present invention against caries-causing bacteria. It was allowed to act for a certain period of time, and the transition after viable bacteria was examined. The test results are shown in FIG. The nanocolloid fine particles and / or nanocolloid water 0 (standard) of the present invention has a function of delaying the growth of Streptococcus mutans MT8148R, but has no inhibitory effect.

Further, the nanocolloid fine particles and / or nanocolloid water 10 (10 times) of the present invention inhibited the growth of Streptococcus mutans MT8148R, but the viable cell count was hardly decreased even after 12 hours of action.
From the above results, it was shown that the nanocolloid fine particles and / or nanocolloid water of the present invention has an action of suppressing the growth of caries-causing bacteria but acts bacteriostatically rather than bactericidally. Furthermore, it was shown that the nanocolloid fine particles and / or nanocolloid water of the present invention also decreased as compared to the control with respect to the effect of Gtase on glucan production. From the above results, even if the nanocolloid fine particles and / or nanocolloid water of the present invention is drunk in a predetermined amount, or drunk for other purposes, it is greatly effective in inhibiting the growth of caries-causing bacteria and fixing it in in vitro tests. The combined inhibitory activity on the production of adherent insoluble glucan involved suggested the usefulness of caries prevention.

(Example 18 Tyronase activity inhibition proof experiment)
Next, “Whitening” was carried out as an example of the effect of inhibiting tyrosinase activity by the inventive nanocolloid fine particles and / or nanocolloid water in the cosmetic field of the present invention.
That is, the key point of whitening is the melanin production inhibitory effect, and this action also makes it possible to prevent spots. Therefore, focusing on the antioxidant action of the nanocolloid fine particles and / or nanocolloid water of the present invention, the production inhibitory effect of melanin causing pigmentation after inflammation and senile pigment spots was examined using tyrosinase activity as an index.

  Thus, the nanocolloid fine particles and / or nanocolloid water (conc) of the present invention were used as basic materials, and ascorbic acid and hydroquinone were used as contrast agents. As tyrosinase, a mushroom-derived (Sigma: 2500 U / mg) was used. As its specific production method, four types of solutions were prepared for verification of the tyrosinase activity inhibitory action.

that is,
(1) tyrosine solution (0.5 mg / ml); addition amount 0.5 ml,
(2) 1 / 15M phosphate buffer (pH 6.8); addition amount 2.0 ml,
(3) Tyrosinase solution (6.0 mg / 100 ml); addition amount 0.5 ml,
(4) Test solution: Nanocolloid fine particles of the present invention and / or nanocolloid water, ascorbic acid, hydroquinone (0.005, 0.01, 0.05, 0.1, 0.5 mg dissolved in buffer); added The volume was 2.0 ml.

  Next, the solutions (1) to (4) were added, incubated at 37 ° C. for 1 hour, and the absorbance (At) at 475 nm was measured. In addition, a phosphate buffer (2 ml) was added instead of each test solution, and the absorbance (Ab) when the same operation was performed was used as a control to determine the inhibition rate. Subsequently, the suppression rate was calculated | required from Ab and At value of this result (suppression rate = (Ab-At) / Ab * 100).

As a result of the above implementation, Table 13 shows the tyrosinase inhibition rate (%) depending on the addition amount of ascorbic acid and hydroquinone solution. Thereby, the tyrosinase inhibition rate by the nanocolloid fine particles of the present invention and / or nanocolloid water (conc.) Was 18.45%.
When this is converted into the amount of each control solution in Table 1, the tyrodinase inhibition rate of the nanocolloid fine particles and / or nanocolloid water of the present invention has an obvious effect corresponding to 0.1 mg of ascorbic acid and 0.007 mg of hydroquinone. Admitted.

  That is, the production of melanin begins with tyrosine, which is one of the amino acids. The tyrosinase verified in this experiment has a two-step reaction from tyrosine to DOPA, which is the first stage of melanin production, and then DOPA to DOPA quinone. It is an enzyme that catalyzes. Therefore, tyrosinase is most important for the production of melanin. From this experiment, ascorbic acid and hydroquinone tyrosinase activities, which are considered effective for pigment spots, were suppressed.

  From the above results, the nanocolloid fine particles and / or nanocolloid water of the present invention has an effect of suppressing the production of melanin that causes pigmentation after inflammation and senile pigment spots, and can act as a bleaching agent. It was suggested.

(Example 19 Experiment on antibacterial effect printing [material] ink)
As described above, the nanoceramic fine particles of the present invention and the nanocolloid water of the present invention have an antibacterial action.

Nowadays, packaged foods such as cup ramen and various cards used by everyone are flooding together, and printed resin cards such as containers and labels are especially bacteria. In order to keep them clean, it is desired to apply antibacterial treatment.
By the way, when antibacterial treatment is applied to these products, it is usually considered that the antibacterial agent is infiltrated into the product. Means to mix the agent are taken.

  By the way, as described above, the nanocolloid fine particles and / or nanocolloid water of the present invention have both an inhibitory effect on growth against caries-causing bacteria in an in vitro test and an inhibitory activity on the production of adherent insoluble glucan, which is largely involved in its establishment. Therefore, the usefulness of antibacterial action is suggested.

  Therefore, the growth inhibitory effect on the causative fungus above: Streptococcus mutans MT8148R was inoculated into a BHI liquid medium and aerobically cultured at 37 ° C. for 24 hours to obtain a bacterial solution. 50 μl of sample solution was added and cultured at 37 ° C. aerobically. After culturing for a specified time, the culture solution is diluted and smeared on BHI, and cultured for 48 hours. After that, the colony forming units (GFU) / ml at each sample concentration and action time are determined from the number of colonies. Prepared to 1.5-5%. 5 to 15% pigment, 15 to 40% synthetic resin, 15 to 35% drying oil, 5 to 30% petroleum solvent, 1 to 10% film strengthening agent, 0.2 to 4 set-off fireproofing agent 0.5%, 1 kg of printing ink formulated to contain 1-10% of dryer and other solvents.

In addition, a printing ink was produced by intercalating silver, an organic material with antibacterial performance, or benzalkonium or cetylpyridinium, an organic substance, with a sparingly soluble phosphate, and various printings were performed using this ink. And showed excellent antibacterial effect.
The above-described antibacterial agent containing the nanoceramic and / or nanocolloid water is not so-called ink, and the same effect can be obtained by applying it to the surface of any printed surface.

(Example 20 health goods, ornaments)
While the present invention has been found to have a remarkable effect by drinking a predetermined amount for a predetermined period as a sports drink, at least a material for an accessory such as a ring or a bracelet in contact with the body or a part thereof In addition, the above-described nanoceramic and / or nanocolloid water of the present invention or the effect of the nanoceramic and / or nanocolloidal water of the present invention and the unique effects of silica (silica stone) and black silica (graphite silica) can be exhibited.

  That is, it is known that the above silica (silica stone) and black silica (graphite silica) emit far infrared rays of 0.3 to 1.4 microns. Colloidal hydrosaccharides have excellent magnetism due to the contained Fe3P and FeSiO4, and this is effective in the treatment of severe cancer pain, polyps, hepatitis, ulcer pain, etc., and low back pain, stiff shoulders, rheumatism, arthritis It has also been found to be effective in treating neuralgia, atopic dermatitis and the like.

  In particular, the effect of this magnetism did not fade even after many years of use, or even when washed with hydrochloric acid, the magnetism could not be removed. It has also been found that the nanoceramic and / or nanocolloid water of the present invention is powdery and conductive and does not pass X-rays (X-rays). Means for contacting the body are a ring, a necklace, a tie pin, an earring, a pierced earring, and the like.

(Example 21 Fertilizer, food, feed)
Further, since the nanoceramic fine particles and nanocolloid water of the present invention have an antibacterial action, they can be used by mixing them with various fertilizers.
For example, the fertilizer is an alkaline composition mainly composed of incinerated ash (oxide) containing Na + ions and carbide containing Na + ions, and the carbide is selected from paper sludge carbide, wood carbide, and rice husk carbide. However, at this time, an appropriate amount of the nanoceramic fine particles or nanocotoid water of the present invention is added.

  At this time, you may mix | blend an alkaline composition with a bark compost so that pH may become larger than 7.5. In this case, the alkali bark has a moisture content of less than 55% by weight with respect to the alkaline bark solid weight (wet weight) and the number of thermophilic bacteria and thermostable bacteria per gram of the alkaline bark solid weight ( It is preferable to carry high-humidity bacteria and heat-resistant bacteria (high-temperature properties) so that each of the high-humidity properties is 102 or more.

Next, in a specific example using this as a fertilizer,
-1- In fruits and vegetables, especially tomatoes, melons, cucumbers, eggplants, strawberries, etc., when the fruit is taken out, the vigor of the trees will surely weaken, and diseases frequently occur at this time. Therefore, by adding (applying) the fertilizer of the present invention a little before the fruit was taken out, the vigor of the tree became healthy and the occurrence of disease could be suppressed. In addition, for example, one melon has a fruit per one in the past, but by implementing the present invention, the tree vigor became stronger and came to bear two to three. Furthermore, the effect of implementing the present invention was that production could be increased by 50% for tomatoes, 50% for cucumbers, and 70-80% for strawberries.

  As for -2-flowers, as a result of the strong tree as described above, it is now possible to ship flowers, especially when roses are cut or cut. Moreover, although there are various methods for transporting fresh flowers for transporting cut flowers, it has been reported that by carrying out the present invention, they have not wilted for more than 7 days from around February to March.

  -3- Furthermore, even if soft vegetables (spinach, komatsuna, etc.) are displayed on the storefront by spraying the nano colloidal water of the present invention before shipping (2-3 days), it is in comparison with the conventional one. Three days were extended, and of course it was reported that the leaves were firm.

  -4-Subsequently, radish, carrot, burdock and the like, which are roots and leaves, are known to have the effect of improving the skin of the crop.

  -5- With regard to general disinfection of soil, in particular, methyl bromide started from DDT, but it has been changed to 2005 methyl bromide use leaf, and there is no change at present. The effect superior to this methyl bromide was demonstrated.

(Example 22: Bathing agent (detergent, etc.) as household goods)
The present invention The nanocolloid fine particles and / or nanocolloid water of the present invention has an excellent feature and effect that it suppresses tyrosinase activity, so that it is not only applied to the face and body, but also a bathtub for bathing When an appropriate amount was mixed in and bathed, the same effect as in the above example was obtained. Further, in the washing of clothes, particularly underwear, when the nanocolloid fine particles and / or nanocolloid water of the present invention were mixed in the finished water, the same effect as in the above example was observed.

(Example 23: Prevention of condensation)
As described above, the nanoceramic fine particles have the effect of preventing the occurrence of so-called dew condensation as described above. Therefore, the nanoceramics particles are particularly suitable for the plastic bottles for soft drinks currently on the market.

  That is, when a low-priced object such as frozen drinking water is stored in a current plastic bottle, the temperature of the outside air is cooled and a phenomenon occurs in which the bottle surface gets wet as fine water droplets. This is because the temperature is drastically decreased particularly on the surface side of the portion in direct contact with the stored item, and condensation occurs there. This phenomenon becomes more pronounced as the outside air temperature and humidity are higher and the time during which the low-frequency sound is stored is longer.

  Increasing the thickness of the PET resin used decreases the amount of condensation, but cannot completely prevent it. In addition, the manufacturing cost increases in proportion to the thickness. As a result of dew condensation, water droplets are generated on the surface of the cold insulation sheet or the cold insulation bag, and it is a common experience that soils clothes and damages other contacting articles.

As a means for preventing this dew condensation, a layer of a dew condensation inhibitor is provided on the bottle or a label wound around the bottle by means such as vapor deposition to prevent dew condensation on the surface. In this case, the wound label is formed by applying the anti-condensation agent using the nanoceramic fine particles and / or nanocolloid water of the present invention and bonding the film and another water absorption layer together.
Here, the water-absorbing layer refers to a fabric product such as cloth, knit, or non-woven fabric, paper such as Japanese paper, artificial leather, a product obtained by bonding a block processed product, or a product obtained by subjecting a vapor-deposited film to direct block processing.

  Therefore, the vapor deposition film is most preferably one deposited on a polyethylene terephthalate (PET) film in terms of physical properties such as strength, but is not limited to this, and other films such as OPP, PC, and PE films can also be used. is there. The thickness of the film is preferably 10 to 100 μm, particularly about 12 to 25 μm. As the anti-condensation agent, the nano-ceramic fine particles of the present invention alone or ordinary aluminum, as well as a combination of chromium, nickel, titanium, tin and the like can be used. The thickness of the deposited film is about 200 to 1000 mm, more preferably 300 to 600 mm.

Even when the PET bottle subjected to the above dew condensation prevention treatment is taken out from a cooled state to a high temperature place, no dew condensation is found in appearance, or even if it is present, it is quite slight. Furthermore, it is possible to remove stains by spraying on glasses such as glasses due to the effect of the surface activity and wiping it off.
(The invention's effect)

  When the effects of the present invention are described as a summary, the nanoceramic fine particles and / or nanocolloid water of the present invention exerts excellent effects on the growth of plants in the lives of animals including humans.

For example, antioxidants can efficiently scavenge radicals such as active oxygen. In particular, colloidal water is composed of inorganic substances and is not used as an energy source in the human body. It has the characteristics of easy absorption and excretion, so it circulates in the blood and acts as a radical scavenger. It is excreted.
Therefore, the antioxidant of the present invention is particularly effective when used as an active pharmaceutical ingredient, a pharmaceutical additive, a food additive, a health food, a cosmetic, or a bath agent.

  Currently, active oxygen-related diseases include cancer, brain diseases, neurological diseases (such as Alzheimer's disease, Parkinson's disease, and cerebrovascular disease), lung diseases (such as pulmonary oxygen poisoning, respiratory tract syndrome, airway hypersensitivity), Myocardial ischemia-reperfusion injury, myocardial infarction, vascular disease (eg, arteriosclerosis), gastrointestinal mucosal injury (eg, gastrointestinal ulcer), liver disease, kidney disease (eg, nephritis, renal failure), eye disease (cataract, retina) Diseases), skin diseases (dermatitis, photosensitivity, etc.), diabetes diseases, immune diseases (AIDS, autoimmune diseases, etc.), antiaging agents, etc. are particularly effective.

  Other than such medical relations, activation of cosmetics and plants as a whole, fertilizer, food, feed, health links, etc., jewelry, pigments, dyes, painting (antibacterial, etc.), stationery, teaching tools, electronic equipment (electromagnetic wave control) , Ideal for clothes (blends) and bathing agents, prevents mercury and lead poisons, and reduces harmful electromagnetic waves emitted from personal computers.

  In other words, if the nanoceramic fine particles and / or nanocolloid water of the present invention is regularly used, hay fever is prevented as a lifestyle-related disease, atopy is prevented, and fatiness is prevented. In addition, it prevents constipation, has anti-rheumatic effects, prevents body edema, and detoxifies common poisons.

  It also prevents allergies, relieves stress, relieves sadness, and relieves nerve fatigue. It also reduces anger, prevents viruses in general, and prevents fungi and mold. Furthermore, it prevents the soreness and prevents so-called blur. In addition, the effect of preventing diarrhea and the bactericidal effect are remarkable. It cures insomnia and prevents cold, while preventing mercury and lead poisons.

  As a specific case, as a result of regularly using the nanoceramic fine particles and / or nanocolloid water of the present invention, a cold was caught but no heat was generated. The cold was cured. Hay fever was cured. The treatment for broken bones, athlete's foot, shortness of breath, and fatigue were cured in a relatively short period of time. There have been reports of effects such as whitening (whitening) in a relatively short period of time when used as a cosmetic. It has been reported that mirrors, glass bottles, etc. help prevent condensation, and when applied to the surface, various products become more glossy and difficult to get dirty.

  Furthermore, spraying on glasses such as glasses due to the effect of its surface activity, the stains are well removed by wiping them off. effective.

Magnetic property graph of nano-ceramics of the present invention Zeta potential graph of colloidal water containing fine nano-ceramics of the present invention Graph showing the time course of edema rate in each group Graph showing the effect of tumor cell growth inhibition Effect graph of the nanocolloid water of the present invention on PGE2 production in large intestine mucosa Abnormal crypt focus (ACF) photo of colonic mucosa Photograph of atypical crypt foci AOM county of the large intestine mucosa Graph of ACF count showing precancerous lesions Graph showing the activity value of the ODC A graph that records the number of writhing for 10 minutes, starting 10 minutes after acetic acid administration to the mouse. Graph of inhibitory effect on carrageenin-induced footpad puroma in rats Graph of brewer's yeast-induced inflammation and analgesic effects in rats Graph showing the effect of anti-inflammatory action Antidiabetic action graph for spontaneously diabetic rats Graph of effects on insulin secretion after maltose intake Graph of effects on insulin secretion after maltose intake Graph showing the effects of caries-causing bacteria

Claims (28)

Nanoceramic fine particles comprising a ceramic fine particle main body made of silicon carbide and / or an unreacted material thereof The unreacted material is one or more selected from silicon dioxide, silicon monoxide, and metal carbide, and can exhibit a radical scavenging inhibitory action efficiently against in vivo radicals. Nanoceramic fine particles according to claim 1 3. The nanoceramic according to claim 1 or 2, wherein the radical to be trapped is one or more selected from active oxides such as superoxide, hydrogen peroxide, hiddlexyl radical, and singlet oxygen. Fine particles In producing nano-ceramic fine particles that can effectively exhibit radical scavenging suppression action against in-vivo radicals, nano-ceramic fine particles characterized by including ceramic fine particles of silicon carbide or / and unreacted substances thereof. Production method The method for producing nanoceramic fine particles according to claim 4, wherein one or more of silicon dioxide, silicon monoxide, and metal carbide is selected as the unreacted material. Claims 4 and 5 are characterized in that the radial to be captured is selected from one or more of active oxygen such as superoxide, hydrogen peroxide, hydroxyl radical, and singlet oxygen. Method for producing nano ceramic fine particles The size of the nano ceramic body is 0.3 microns or less, has ferromagnetism, and has a predetermined kilo-ohm conductivity. Method 8. The nanoceramic colloidal water according to any one of claims 1 to 7, which is produced by dissolving the nanoceramic fine particle main body in normal water or the like and filtering it. 9. The method for producing nano-ceramic colloidal water according to claim 1, wherein the nano-ceramic is dissolved in normal water and filtered. The method for producing nano-ceramic colloidal water according to any one of claims 1 to 9, wherein the material is formed into a spherical shape when producing the nano-ceramic used in the colloidal water containing the nano-ceramic fine particles. 11. The nanoceramic fine particles, the colloidal water thereof, and the method for producing the same according to claim 1, wherein the nanoceramic fine particle main body and / or the nanocolloid water main body thereof are used as an antioxidant. 11. The nano-ceramic fine particles and nano-colloidal water according to claim 1, wherein the nano-ceramic fine particle main body and / or the nano-colloid water main body is used as an anti-inflammatory agent. 11. The nanoceramic fine particles and nanocolloidal water according to claim 1, wherein the nanoceramic fine particle main body and / or nanocolloid water main body thereof is used as an analgesic. 11. The nanoceramic fine particles and nanocolloid water according to claim 1, wherein the colloidal water containing the nanoceramic fine particles is used as an anticancer agent. 11. The nanoceramic fine particles and nanocolloid water according to claim 1, wherein the nanoceramic fine particles and / or the nanocolloid water main body is used as an aldose reductase inhibitor for diabetic complications. Production method 11. The nano-ceramic fine particles and nano-colloidal water according to claim 1, wherein the nano-ceramic fine particle main body and the nano-colloid water main body are used as a disaccharide-degrading enzyme inhibitor. 11. The nano ceramic fine particles and nano colloid water according to claim 1, wherein the nano ceramic fine particles main body and / or nano colloid water main body thereof are used as a melanin production inhibitor. 11. The nano ceramic fine particles and nano colloidal water according to claim 1, wherein the nano ceramic fine particle main body and / or the nano colloid water main body is used as an antiallergic agent, and a method for producing the same. 11. The nano ceramic fine particles according to claim 1, wherein the nano ceramic fine particle main body and / or nano colloidal water main body thereof are mixed into a health drink such as a drink or a soft drink. And nano colloidal water and method for producing the same 11. The nano ceramic fine particle main body and / or the nano colloid water main body is used as an additive containing various breeding foods of humans or livestock or perfumes thereof, and coloring agents. Nano-ceramic fine particles, nano-colloidal water, and production method thereof 11. The nano ceramic fine particles and nano colloids according to claim 1, wherein the nano ceramic fine particle main body and / or the nano colloid water main body are used as other fertilizers for improving other soils for growing various plants. Water and its manufacturing method The nano ceramic fine particle main body and / or the nano colloidal water main body is used by fusing, pasting or embedding a bracelet, a ring, a necklace, an earring, a pierced earring or other decorative item. 11. Nano-ceramic fine particles and nano-colloidal water according to claim 1 and a method for producing the same, which are used for various health goods 11. The nanoceramic fine particle and nanocolloid according to claim 1, wherein the nanoceramic fine particle main body and / or the nanocolloid water main body thereof is used as a cloth or the like used for clothes or the like by means of twisting, blending, or the like. Water and its manufacturing method The nano ceramic fine particle main body and / or the nano colloid water main body is used as an agent for various germs, fungi, etc. by means of applying ink on printed matter including various resin credit cards or on the printed matter. 11. The nanoceramic fine particles and nanocolloidal water according to claim 1 and a method for producing the same 2. The nano ceramic fine particle main body and / or the nano colloid water main body is used for cosmetics such as various nutritional creams and cleansing creams by means of mixing, and used for bathing agents. To 10 ceramic nanoparticle and nanocolloid water and method for producing the same The above-mentioned nano ceramic fine particle main body and / or nano colloidal water main body is made of resin bottles, beer cans (bottles), window glass including the front of automobiles, mirrors, room walls, furniture and so-called dew condensation sites. 11. Nano-ceramic fine particles and nano-colloidal water according to claim 1 and a method for producing the same according to claim 1 2. The high frequency electromagnetic wave absorption characteristic of the silicon carbide or / and its unreacted material, and a synthetic reaction is performed using a high frequency electromagnetic wave irradiation device of 2 GHz or more and using the raw material itself as a heating element. To 10 ceramic nanoparticle and nanocolloid water and method for producing the same The nano ceramic fine particle main body and / or the nano colloid water main body is used in a variety of electronic devices and the like, and is installed in the vicinity of a device having an adverse effect such as electromagnetic waves or radio wave interference. Item 10. The nanoceramic fine particles and nanocolloid water according to Item 1 to 10, and a method for producing the same

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