JP2005224106A5

JP2005224106A5 -

Info

Publication number: JP2005224106A5
Application number: JP2004032877A
Authority: JP
Filing date: 2004-02-10
Publication date: 2006-11-30
Anticipated expiration: 2024-02-10

Description

本発明の細胞培養装置は、比増殖速度を得たときの細胞の種類及び培養条件を前記比増殖速度に関連付けて格納したデータベース部と、培養によって得たい必要細胞数及び時期を入力する入力部と、設定された環境で細胞の培養を行うための細胞培養部と、この細胞培養部に収容された培養容器内の細胞の培養状態をカメラで撮影して画像データを作成する画像データ作成部と、細胞培養部に収容された培養容器へ培養液の供給及び排出を行う培養液給排部と、データベース部、入力部、細胞培養部、画像データ作成部及び培養液給排部に電気的に接続された制御部とを備え、制御部は、培養を開始するときの初期細胞数と、必要細胞数及び必要時期とに基づいて比増殖速度を算出し、培養する細胞の種類とに基づいてデータベース部から最も近い比増殖速度を選択し、この選択した比増殖速度に対応する培養条件の情報を取得し、この取得した培養条件の情報に基づいて、細胞培養部及び前記培養液給排部の動作を制御する構成とすることにより上記課題を解決する。 Cell culture device of the present invention includes a database unit for the type and culture conditions of a cell when the obtained specific growth rate is stored in association with the specific growth rate, the input unit for inputting the required number of cells and timing to be obtained by culturing And a cell culture unit for culturing cells in a set environment, and an image data creation unit for creating image data by photographing the culture state of the cells in the culture container accommodated in the cell culture unit with a camera And a culture solution supply / discharge unit that supplies and discharges the culture solution to and from the culture container accommodated in the cell culture unit, and a database unit, an input unit, a cell culture unit, an image data creation unit, and a culture solution supply / discharge unit. A control unit connected to the control unit, the control unit calculates a specific growth rate based on the initial cell number when starting culture, the required cell number and the required time, and based on the type of cells to be cultured From the database department Select closer specific growth rate, obtains the information of the culture conditions corresponding to the selected specific growth rate, based on the information of the acquired culture conditions, controls the operation of the cell culture unit and the cultures supply and discharge unit The above-described problem is solved by adopting a configuration to do so.

Claims

A database unit storing cell types and culture conditions when specific growth rate is obtained in association with the specific growth rate, an input unit for inputting the required number and period of cells to be obtained by culture, and cells in a set environment A cell culture unit for culturing the cells, an image data creation unit for creating image data by photographing the culture state of the cells in the culture container accommodated in the cell culture unit, and the cell culture unit Electrically connected to the culture solution supply / discharge unit for supplying and discharging the culture solution to and from the culture vessel, and the database unit, the input unit, the cell culture unit, the image data creation unit, and the culture solution supply / discharge unit The control unit calculates the specific growth rate based on the initial cell number when starting culture, the necessary cell number and the necessary time, and based on the type of cells to be cultured. The database The specific growth rate closest to the cell portion is selected, information on the culture conditions corresponding to the selected specific growth rate is acquired, and the cell culture unit and the culture solution supply are obtained based on the acquired information on the culture conditions. A cell culture device that controls the operation of the drainage section.

The control unit calculates the number of cells based on the image data from the image data generation unit during cell culture, calculates a specific growth rate based on the calculated number of cells, and calculates the calculated specific growth rate. The number of cells obtained at the required time calculated based on the required number of cells is compared to determine whether the required number of cells can be obtained at the required time. When the number of cells cannot be obtained, the specific growth rate is calculated based on the calculated number of cells, the required number of cells and the required time, and the closest to the database unit based on the type of the cells. Select a specific growth rate, acquire information on a new culture condition corresponding to the selected specific growth rate, and operate the cell culture unit and the culture solution supply / discharge unit based on the acquired culture condition information It is characterized by being controlled Cell culture device according to claim 1 that.