JP2005176700A - Tooth-related reagent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide examination techniques compatible with each tooth and periodontal tissue including those of utilizing reagents for testing each tooth and periodontal tissue in high accuracy, particularly utilizing reagents for culturing bacteria, namely media used for culturing bacteria. <P>SOLUTION: A tooth-related reagent or a culture medium is provided, being characterized by comprising a sampling means using a paper point, an input/output means for a gas from a reagent or a reaction reagent, a colony level judging means, a gas-measuring means for quantification by gas chromatography and a quick-measuring means, and the like. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention is a technique for effectively using a reagent for examining bacteria or flora of teeth and periodontals, and particularly a technique for effectively using a reagent for culturing bacteria, that is, a medium used for bacterial culture.

  Conventionally, there was no reagent or medium corresponding to the tooth type.

Conventional reagents are not contained in petri dishes or in vials and do not correspond to examination sites such as teeth and periodontal tissue.

1 [Means of Claim 1]
The dental reagent of claim 1 is:
It is characterized by comprising tooth type reagent means for making the reagent correspond to the tooth type.
2 [Means of claim 2]
The culture medium of claim 1
The tooth type reagent means includes an input / output means for inputting / outputting a paper point, a gas from the reagent or a reaction reagent.
3 (Means of claim 3)
The tooth reagent or medium of claim 3 is:
It is provided with a colony level determination means.
4 (Means of claim 4)
The dental reagent or medium of claim 4 is:
Gas measurement means is provided.
5 (Means of Claim 5)
The dental reagent or medium of claim 5 is:
Quick measurement means is provided.

1 [Operation and effect of claim 1]
The dental reagent of claim 1 is:
Since it is provided with a tooth type reagent means for making the culture medium correspond to the tooth type, an inspection corresponding to a specific tooth or periodontal tissue can be performed.
2 [Operation and effect of claim 2]
The culture medium of claim 1
Since the tooth type reagent means includes an input / output means for inputting / outputting a paper point, a gas from the reagent or a reaction reagent, etc., the paper point is collected in an inspection corresponding to a specific tooth or periodontal tissue. Can be inspected. In addition, it is possible to easily supply reagents, and collect reaction gases and reactants between the reagent and the collected material.
3 [Operation and effect of claim 3]
The tooth reagent or medium of claim 3 is:
Since the colony level determining means is provided, the colony level can be easily determined in an inspection corresponding to a specific tooth or periodontal tissue.
4 [Operation and effect of claim 4]
The dental reagent or medium of claim 4 is:
Since it is provided with a gas measuring means, qualitative, quantitative, and identification of gas generating bacteria and the like can be performed. It can also be used for testing bad breath and disease.
5 [Operation and effect of claim 5]
The dental reagent or medium of claim 5 is:
Since it has a quick measurement means, the culture test result can be understood quickly. If applied directly to the oral cavity, it is possible to test and measure the pathogenic bacteria in the oral cavity in real time.

The tooth type reagent of this invention is demonstrated based on the Example or modification shown in FIGS.
An embodiment adopts a tooth type reagent means that associates a tooth type with a reagent.

[Configuration of Example]
In FIG. 1, the reagent holding means for holding the medium is made of a transparent resin. The tooth type which can specify a tooth type is printed on the back surface of the resin. This reveals which part of the reagent is which tooth part in the oral cavity. This is an example of a tooth type reagent means. Then, the medium is held in the reagent holding means.

In other words, FIG. 1 discloses a square petri dish molded from plastic. The tooth type reagent means is printed on the bottom of the petri dish.

[effect]
The tooth type reagent means clarifies which part of the reagent is which tooth part in the oral cavity. Further, in the conventional circular petri dish, the sampling width of each tooth type tends to interfere as it goes to the center, but if the tooth type reagent means is employed, the interference disappears.

[Modification]
Any reagent holding means may be used as long as the reagent can be held, such as a band-type or flat plate-type reagent holding means such as a petri dish (reagent holding means).

The shapes shown in FIGS. 2, 3, 4, 5, and 7 may be employed.

The above means are selected, used and manufactured by an operator or manufacturer.

  The first embodiment is an example of a tooth type reagent means.

[First Embodiment]
The first embodiment is an example in which the tooth type reagent means is printed on the bottom upper part of the reagent holding means.

[Constitution]
In FIG. 1, it comprises a reagent holding means and a tooth type reagent means.

Here, the reagent holding means is made of a transparent resin, and a tooth type is printed on the outer bottom surface thereof, which serves as a tooth type reagent means for associating the reagent with the tooth type. The reagent selected by the operator is held on the reagent holding means. This reagent may be an agar medium, a broth medium or a semi-fluid medium. However, in the case of broth, it is desirable to provide a partition for each tooth type so as not to interfere.

[Operation]
Tooth in the oral cavity, for example, upper right third (upper right canine) is traced with a sterilized cotton swab which is an example of a sampling means. The swab is applied to the left three reagent parts as shown in FIG. Cultivate for 48 hours and observe colonies. As an example, if chromoagar is used as a reagent, the degree of Candida infection corresponding to each tooth can be determined.

[effect]
It enables examination of bacteria corresponding to individual teeth and periodontal tissue here. As a result, pathogenic bacteria infecting individual teeth and periodontal tissues can be identified, and the standard of treatment and the degree of treatment can be determined.

[Modification]
As shown in FIG. 2, tooth type reagent means corresponding to all the upper, lower, left and right teeth may be employed. At this time, position information means for describing position information such as R (right) and L (left) may be employed. Furthermore, personal information means for printing the patient name, the collection date, etc. may be employed. This can be realized by securing a description space.

Moreover, a vertical type may be used as shown in FIG. Here, FIG. 4 discloses two examples of a right diagram and a left diagram.

The second embodiment discloses an example in which an input / output means is provided together with a tooth type reagent means.

[Second Embodiment]
An example is disclosed in which a paper point is used as a sampling means, which is input from an input / output means and cultured.

[Constitution]
In FIG. 3, it comprises a reagent holding means, a tooth type reagent means and an input / output means. The reagent holding means is a petri dish made of transparent resin. And the tooth type reagent means is stuck on the bottom by printing or a seal. And the input / output means is affixed to the reagent holding means by attaching a film of polyethylene or the like to make a hole. At this time, instead of the film, a hole may be made in a resin lid or cover to serve as input / output means.
As an example, Porphyromonas gingivalis broth is dispensed in the reagent holding means. When the broth is adopted, there is a limit for each tooth type, and broth is dispensed so as not to interfere.

[Operation]
Insert paper points into the periodontal pocket or root canal. The inserted paper point is inserted into the input / output means in FIG. Then, culture is performed and the color of the reagent and the shape of the colony are observed.

[effect]
It enables examination of bacteria corresponding to individual teeth and periodontal tissue here. As a result, pathogenic bacteria infecting individual teeth and periodontal tissues can be identified, and the standard of treatment and the degree of treatment can be determined.

[Modification]
The reagent used was broth, but it could be a semi-fluid or agar-based medium. In this case, it may be dispensed with a threshold or without a threshold.

Here, when a threshold is provided, this input / output means is used when the room containing the reagent in each tooth type is completely separated by this threshold and a threshold that does not interfere with each other is provided. Thus, the gas after the culture can be collected. Reagents can also be collected.

Here, in the case of gas, an opening / closing means for temporarily opening / closing the input / output means may be used. As an example of the opening / closing means, an opening / closing means using a cellophane tape or the like is convenient. Of course, a lid made of resin or the like may be used as the opening / closing means. These prevent deterioration of the reagent during transport of the reagent and prevent gas leakage during culture. Moreover, this opening / closing means may be comprised with the film fixed after the data provision to the reagent from an intraoral area. In this case, the gas output may be a one-time opening / closing means for breaking and collecting the film with a pipette. Further, the input / output means and the opening / closing means may not be provided individually, but may be ones that can be removed as a whole like an anaerobic jar. The amount of phenylacetic acid may be quantitatively analyzed by gas chromatography on the gas collected with this pipette. Then Porphyromonas gingivalis can be quantified. Of course, qualitative measurement may be used, but it goes without saying that quantitative measurement has higher diagnostic accuracy.

Here, the example which employ | adopted the gas measurement means for detecting the gas which generate | occur | produces from the microbe in a culture solution like FIG. 7 is disclosed. The gas measuring means includes a measuring room and a predetermined wavelength transmission window. Here, it is best to let the culture room and the gas measurement room communicate with each other with a gas permeable membrane, but a simple hole may be used. In the case of a simple pore, the reagent is preferably a solid or semi-fluid. When the reagent is liquid, a liquid impermeable gas permeable membrane is preferable.

As described above, the paper point is input from the input / output means and cultured. At this time, the input / output means is closed by an opening / closing means such as a film, so that the gas generated from the culture solution is filled. At this time, only the gas enters the measurement room by the gas permeable membrane. Since both sides of this room are formed with infrared transmissive windows such as BaF2 (barium fluoride), visible, ultraviolet and infrared spectroscopic analysis of the gas in this room can be easily performed. As a specific example, a paper point from the gingival crevice of a periodontal disease patient is inserted into a Porphyromonas gingivalis selective medium or the like through an input / output means and closed by an opening / closing means. Then, ultraviolet, visible, or infrared spectroscopic analysis is performed by passing the analysis beam through the gas in the measurement room through the BaF ₂ window. Thereby, the tooth type concentration of Porphyromonas gingivalis can be easily measured. In addition, the determination of Porphyromonas gingivalis can be performed very rapidly.

Here, the quick measurement means includes the beam measurement means and display means, or the measurement means and analysis means, or both. As a specific example, Porphyromonas gingivalis uses 2336 cm <-1> and 2361 cm <-1> infrared beams in the gas measuring means, and its absorption intensity is measured in the measuring means. At this time, either 2336 cm −1 (strength Ir), 2361 cm −1 (strength Im) or both of them may be compared with an appropriate internal standard to obtain a Porphyromonas gingivalis concentration. If the concentration is high, the risk may be high. Furthermore, the reference is measured by inserting a measurement reference material into the measurement room. For example, it is compared with this constituent curve. (Analysis means)

As an example, the analyzing means compares the two intensity values. If Im / Ir is about 1, the risk of Porphyromonas gingivalis is high. Let Im / Ir <1 be a small risk. Of course, the analysis means may adopt stepwise evaluation values. Less than 0.5 is low risk, 0.5 to 1.0 is in risk, etc.
At this time, as the Porphyromonas gingivalis selection medium, ABCM agar medium (rabbit hemolysis 2.5%) or ABCA agar medium (rabbit hemolysis 2.5%) supplemented with BGA prescription or Porphyromonas gingivalis selective antibiotic was used. Any medium may be used according to the spirit of the present invention.

Here, the gas in the oral cavity may be collected with a gas syringe, analyzed with a gas chromatograph, ultraviolet, visible, or infrared spectroscopic analyzer, and analyzed with the analyzing means to obtain a periodontal disease risk.

Furthermore, a known peak of phenylacetic acid may be measured, and the value may be compared with an internal standard value. Alternatively, a reference substance may be inserted into the measurement room of the gas measuring means, or a reference substance having a known reference concentration may be coated on the window, and the analysis means may perform comparison and perform quantification or qualification using the reference substance as a reference. (Reference means)

Further, since the volume of the measurement room and the concentration of the coated reference substance are known here, quantitative determination can be performed by the analysis means. In this case, the analyzing means may be a virtual means on a computer equipped with a known concentration calculating means based on the substance ratio from a known calibration curve function.

Here, the opening / closing means may include a valve for reducing the pressure when the pressure exceeds a certain pressure.

3rd Example discloses the example provided with the colony level determination means with the tooth type reagent means.

[Third Embodiment]
An embodiment includes a colony level determination unit that easily and accurately determines the colony level.

[Constitution]
As shown in FIG. 5, the petri dish is drawn on the upper part, and a petri dish is created in which a horizontal line is drawn on the lower part. This horizontal line is a colony level determination means. Here, FIG. 5 discloses three examples: an upper diagram, a middle diagram, and a lower diagram.

[Operation]
The paper point inserted into the periodontal pocket is placed at the bottom of FIG. 5, the collection site is traced with a straight probe, and the traced part is traced from the bottom to the top. Then incubate. This tracing method is described in detail in Japanese Patent Application No. 2002-137841.

[effect]
By examining how much the concentration of the collected bacteria is in the section divided by the horizontal line, the concentration of the bacteria, that is, the colony level is easily found.

[Modification]
As the colony level, a single stage or two or more stages may be adopted. In this case, the line drawing may be any printing or drawing as long as it is in accordance with the gist of the present invention.

[General modification]
A cotton swab, a probe, a brush, or the like may be used as a means for collecting from a living body such as the oral cavity. Further, the Japanese Patent Application No. 2003-376346 vibration brush or the brush by the Japanese Patent Application No. 2003-393192 vibration brush, that is, these DirectBrushes (direct brushes) may be used as sampling means.

  In the above embodiment, a medium is used as a reagent, but a drug for detecting a pathogenic factor, a pH reagent, or the like may be used. When a pH reagent is used, the plaque pH value of the tooth here is known. Further, dental plaque of individual teeth may be collected and applied to a dental reagent means, 5% sucrose solution may be added, and the reaction of the pH reagent may be observed. In this case, the caries risk in each individual tooth is revealed. Alternatively, saliva and gingival crevicular fluid from the teeth may be collected to detect the saliva buffering capacity of each tooth. A commercially available reagent such as Periocheck may also be used.

The culture medium in the tooth type reagent means includes the above-described test reagent. Furthermore, the tooth formula for clarifying the tooth type may be printed anywhere on the reagent holding means. As an example, when the culture medium or reagent is opaque, it is easy to see if it is printed on the top without printing on the bottom surface, or the tooth type reagent means is printed on the lid or the like. Further, the tooth type reagent means may be any type of resin molding letter, seal, baking letter, cutting letter, and the like in accordance with the spirit of the present invention.

The above embodiments or modifications may be implemented alone or in combination. Moreover, you may use for another use. As an example, a reagent holding means may be inserted into the case as shown in FIG. 6 to prevent moisture volatilization or contamination. For example, the case, lid or wrapping may be used in combination. May be used in other examples. Furthermore, each means may be carried out alone or in combination. Combinations may be freely combined.

It enables examination of bacteria corresponding to individual teeth and periodontal tissue here. As a result, pathogenic bacteria infecting individual teeth and periodontal tissues can be identified, and the standard of treatment and the degree of treatment can be determined. As a result, a very large effect such as a dramatic improvement in dentistry can be obtained.

An example of a tooth type reagent means. An example of a tooth type reagent means. An example of a tooth type reagent means. An example of a tooth type reagent means. An example of a tooth type reagent means. An example of a cover of a tooth type reagent means. An example of a tooth type reagent means.

Claims (5)

A tooth type reagent is a tooth type reagent provided with a tooth type reagent means for making a reagent correspond to a tooth type. The dental reagent of claim 1 is:
A tooth type reagent provided with input / output means for inputting / outputting paper points, gas from the reagent or reaction reagent in the tooth type reagent means. The tooth reagent or medium of claim 1 or claim 2,
A tooth type reagent or medium comprising a colony level determination means. The tooth type reagent or medium according to any one of claims 1 to 3,
A tooth type reagent or medium comprising a gas measuring means. The tooth type reagent or medium according to any one of claims 1 to 4,
A tooth type reagent or culture medium comprising a rapid measurement means.

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