JP2005151812A - Suspension stirring culture apparatus - Google Patents

Suspension stirring culture apparatus Download PDF

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JP2005151812A
JP2005151812A JP2003390956A JP2003390956A JP2005151812A JP 2005151812 A JP2005151812 A JP 2005151812A JP 2003390956 A JP2003390956 A JP 2003390956A JP 2003390956 A JP2003390956 A JP 2003390956A JP 2005151812 A JP2005151812 A JP 2005151812A
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stirring
rotating shaft
coil spring
culture vessel
stirring tube
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Japanese (ja)
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Masatake Hasobe
正豪 羽曽部
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Japan Science and Technology Agency
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Japan Science and Technology Agency
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Priority to JP2003390956A priority Critical patent/JP2005151812A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis

Abstract

<P>PROBLEM TO BE SOLVED: To provide a suspension stirring culture apparatus designed to regulate tilt angle of a stirring tube according to the shape of a culture vessel, continue stirring even when carried and have a high stirring ability and a simple structure. <P>SOLUTION: The stirring tube 2 composed of a flexible material and having the closed lower end is hung into the interior of the culture apparatus 1 and a coil spring 3 is installed in the axial direction in the stirring tube 2. A rotating shaft 4 rotated and driven with external power connected to the top and deformable into a shape having the bent lower end is inserted into the center of the coil spring 3. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

本発明は、動物細胞や微生物の浮遊攪拌培養に用いる浮遊攪拌培養装置に関する。   The present invention relates to a suspension stirring culture apparatus used for suspension stirring culture of animal cells and microorganisms.

動物細胞の浮遊攪拌培養では、細胞に物理的なダメージを与えず、且つ、できるだけ分散させて細胞を懸濁させることが重要である。このため、既に種々の浮遊攪拌培養装置が開発されているが、単離浮遊細胞の分散を目的とする攪拌方式のものと、細胞を接着させたマイクロキャリアを浮遊懸濁させる攪拌方式のものとがある。
いずれも、溶液流動性に加え、攪拌により剪断力を与えて細胞を凝集させない仕様となっているが、後者は、大きめの攪拌翼で低速攪拌して、剪断力を小さく抑えながら、キャリアを含む液体培地を効果的に流動させるようになっている（例えば、特許文献１参照）。 In suspension stirring culture of animal cells, it is important to suspend the cells by dispersing them as much as possible without causing physical damage to the cells. For this reason, various suspension agitation culture apparatuses have already been developed. The agitation method is intended to disperse isolated suspended cells, and the agitation method is a suspension method in which microcarriers to which cells are adhered are suspended and suspended. There is.
In addition to the solution fluidity, the specifications are such that the cells are not aggregated by applying shearing force by stirring, but the latter includes a carrier while keeping the shearing force small by stirring at low speed with a large stirring blade. The liquid medium is effectively flowed (see, for example, Patent Document 1).

このような攪拌翼を用いた培養装置は、容器の蓋を中心軸で貫通し、中心軸の下端部に円筒状の回転軸をはめ込み、攪拌翼を有するマグネット棒で回転軸を直角に貫通し、容器の下方に設置した回転磁石でマグネット棒及び攪拌翼を回転させるようになっている。
ところが、このものは、回転磁石が組み込まれたスターラ台に培養容器を載置しなければ、攪拌翼を回転させることができないので、持ち運ぶ際には攪拌が停止されてしまい、この間に細胞が凝集する虞がある。
また、太い回転軸及び大きく張り出した攪拌翼によって容器内の容積が減少してしまい、少量の攪拌培養には適しておらず、容器の寸法に応じて異なる攪拌翼を用いなければならない。 Such a culture apparatus using a stirring blade penetrates the lid of the container with a central axis, fits a cylindrical rotating shaft at the lower end of the central shaft, and penetrates the rotating shaft at right angles with a magnet rod having a stirring blade. The magnet rod and the stirring blade are rotated by a rotating magnet installed below the container.
However, in this case, the stirring blade cannot be rotated unless the culture vessel is placed on a stirrer base in which a rotating magnet is incorporated. Therefore, stirring is stopped when carrying, and cells are aggregated during this time. There is a risk of doing.
Further, the volume in the container is reduced by the thick rotating shaft and the overhanging stirring blade, which is not suitable for a small amount of stirring culture, and different stirring blades must be used depending on the dimensions of the container.

さらに、容器内へ紐で永久磁石攪拌子を吊り下げ、容器の外方に設置されたソレノイドで永久磁石攪拌子を振り子運動させる撹拌装置が知られている（例えば、特許文献２参照）。
しかし、この撹拌装置も、培養容器をソレノイドの間に設置しなければならないので、培養容器を持ち運ぶ際には攪拌が停止されてしまう。また、攪拌子が往復するだけなので攪拌能力が低く、攪拌子が容器の側壁に衝突しやすくて破損する虞があった。 Furthermore, a stirring device is known in which a permanent magnet stirrer is suspended in a container with a string, and the permanent magnet stirrer is moved in a pendulum manner by a solenoid installed outside the container (see, for example, Patent Document 2).
However, this agitation apparatus also requires that the culture vessel be installed between solenoids, so that the agitation is stopped when carrying the culture vessel. Further, since the stirring bar only reciprocates, the stirring ability is low, and the stirring bar may easily collide with the side wall of the container and may be damaged.

特開平４−２７８０７６号公報JP-A-4-278076 特開平６−１４０２７１号公報JP-A-6-140271

この発明は、培養容器の形に応じて攪拌チューブの傾斜角度を調整でき、持ち運び時にも攪拌を継続することが可能で、攪拌能力が高く、構造が簡単な浮遊攪拌培養装置を提供することを課題とする。   The present invention provides a floating agitation culture apparatus that can adjust the inclination angle of the agitation tube according to the shape of the culture vessel, can continue agitation even when carried, has a high agitation ability, and has a simple structure. Let it be an issue.

本発明の浮遊攪拌培養装置は、培養容器の内部へ柔軟素材より成る攪拌チューブを吊り下げ、該攪拌チューブ内にコイルスプリングを軸方向に沿って設置し、上端部に接続された外部動力で回動駆動され、且つ、変形可能な回動軸を前記コイルスプリングの中心に挿通してある。
回動軸の下部を培養容器の寸法に応じて曲げて、攪拌チューブ及びコイルスプリングの下部を斜めに傾斜させ、回動棒を回動させることにより、攪拌チューブの下部を円周方向に移動させ、培養容器内の液体培地を攪拌する。
前記外部動力は、前記培養容器の上蓋に着脱自在に設置されたモータとし、該モータの出力軸と前記回動軸の上端部とを連結しても良い。
前記攪拌チューブの下端部に磁石を装着しても良い。 The suspension agitation culture apparatus of the present invention suspends an agitation tube made of a flexible material inside a culture vessel, installs a coil spring along the axial direction in the agitation tube, and rotates with external power connected to the upper end. A rotational shaft that is driven and deformable is inserted through the center of the coil spring.
The lower part of the stirring tube is bent according to the size of the culture vessel, the lower part of the stirring tube and coil spring is inclined obliquely, and the lower part of the stirring tube is moved in the circumferential direction by rotating the rotating bar. Stir the liquid medium in the culture vessel.
The external power may be a motor detachably installed on the upper lid of the culture vessel, and the output shaft of the motor may be connected to the upper end of the rotating shaft.
A magnet may be attached to the lower end of the stirring tube.

請求項１に係る発明によれば、培養容器を持ち運んでいる間も外部動力によって回動軸を回転させることにより、攪拌を継続することができるので、運搬中に細胞が凝集する心配がない。
また、攪拌チューブと回動軸との間にはコイルスプリングが介在されているため、このコイルスプリングが緩やかに回転することにより、回動軸と攪拌チューブとの摩擦で攪拌チューブが損傷を受けるのを防ぐことができる。 According to the first aspect of the invention, since the agitation can be continued by rotating the rotating shaft by external power even while the culture vessel is being carried, there is no fear that cells are aggregated during transportation.
In addition, since a coil spring is interposed between the stirring tube and the rotating shaft, the stirring tube is damaged due to friction between the rotating shaft and the stirring tube due to the gentle rotation of the coil spring. Can be prevented.

さらに、培養容器の内部には攪拌チューブが吊り下げられているだけなので嵩張らず、口径の小さな培養容器でも使用できると共に、培養容器内の容積が大きく減少することが無く、回動軸の下部の傾斜角度を変えることによって、培養容器の径の違いに対応することが可能である。
また、構造が単純なので、製造コストが低廉で隅、組立・洗浄等の取り扱いが容易で、攪拌チューブの下部が円周に沿って移動するので、培養容器の底面中心部に細胞の凝集や集合が生ずることが少ない。
請求項２に係る発明によれば、従来のものと同様に、スターラ台上に設置し、回転磁石で磁石を移動させることにより攪拌を行うことも可能となる。 Furthermore, since only the stirring tube is suspended inside the culture vessel, the culture vessel is not bulky and can be used with a small-diameter culture vessel. By changing the inclination angle, it is possible to cope with the difference in the diameter of the culture vessel.
In addition, since the structure is simple, the manufacturing cost is low, handling of corners, assembly and cleaning, etc. is easy, and the lower part of the stirring tube moves along the circumference, so that cells aggregate and collect at the center of the bottom of the culture vessel. Is less likely to occur.
According to the second aspect of the present invention, similarly to the conventional one, it is possible to stir by installing on a stirrer base and moving the magnet with a rotating magnet.

以下、本発明の実施例を図面に基づいて詳細に説明する。
本発明の浮遊攪拌培養装置は、図１に示すように、培養容器１と、培養容器１の内部へ吊り下げられた攪拌チューブ２と、攪拌チューブ２内にその軸方向に沿って設置されたコイルスプリング３と、コイルスプリング３の中心に挿通され、その上端部に接続された外部動力によって回動駆動される回動軸４とを備える。
培養容器１の上面は開口しており、この開口面に上蓋５が着脱自在に装着される。上蓋５の中心には貫通孔６が形成され、貫通孔６には、高圧滅菌可能な素材より成る筒部材７が、培養容器１の内外を連通し、且つ、貫通孔６の周縁に密着するように挿通される。 Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
As shown in FIG. 1, the suspension agitation culture apparatus of the present invention is installed along the axial direction in a culture vessel 1, a stirring tube 2 suspended inside the culture vessel 1, and the stirring tube 2. A coil spring 3 and a rotating shaft 4 that is inserted into the center of the coil spring 3 and is driven to rotate by external power connected to an upper end portion thereof are provided.
The upper surface of the culture vessel 1 is open, and the upper lid 5 is detachably attached to the open surface. A through hole 6 is formed at the center of the upper lid 5, and a cylindrical member 7 made of a material that can be sterilized at high pressure communicates with the inside and outside of the culture vessel 1 and is in close contact with the periphery of the through hole 6. Is inserted as follows.

攪拌チューブ２は、耐熱無毒性の柔軟素材（例えばシリコン）より成り、その上端部が筒部材７の下部外周に嵌合されている。
また、攪拌チューブ２の下端部には、円柱状の磁石８が装着される。磁石８の外面はポリ四フッ化エチレンで被覆され、その上部を攪拌チューブ２の下端部へ挿入してある。
なお、攪拌チューブ２の内径は、筒部材７の下部の外径及び磁石８の外径よりもやや小さく形成され、無理に嵌め込むと簡単には外れないようになっている。 The stirring tube 2 is made of a heat-resistant and non-toxic flexible material (for example, silicon), and its upper end is fitted to the lower outer periphery of the cylindrical member 7.
A cylindrical magnet 8 is attached to the lower end portion of the stirring tube 2. The outer surface of the magnet 8 is covered with polytetrafluoroethylene, and the upper part thereof is inserted into the lower end of the stirring tube 2.
The inner diameter of the stirring tube 2 is formed slightly smaller than the outer diameter of the lower part of the cylindrical member 7 and the outer diameter of the magnet 8 so that it cannot be easily removed when it is forcibly fitted.

コイルスプリング３は、攪拌チューブ２の内径よりもやや細く、攪拌チューブ２の全長に亘って挿入され、その上端部は培養容器１の外部へ延びて、筒部材７の上端から僅かに突出している。
回動軸４は、ピアノ線等の細くて変形可能な線材より成り、コイルスプリング３よりもやや長く形成される。また、コイルスプリング３から突出した回動軸４の上端には、コイルスプリング３の直径よりも径大の継手１０が装着される。
そして、回動軸４をコイルスプリング３に通した後、回動軸４の下部を下端から適宜高さで折り曲げて適宜角度に傾斜させると、回動軸４の外側のコイルスプリング３及び攪拌チューブ２の下部も傾斜する。この状態で回動軸４の上端部を回動させると、回動軸４の下端及びこれを被覆した攪拌チューブ２の下端が円周を描くように回転する。
なお、この時、軸芯回りに回動する回動軸４と攪拌チューブ２との間には、相対的な回転が生ずるが、両者の間に介在されたコイルスプリング３が、回動軸４の回動に伴って緩やかに回転するため、大きな摩擦力が発生することはない。 The coil spring 3 is slightly thinner than the inner diameter of the stirring tube 2 and is inserted over the entire length of the stirring tube 2, and its upper end extends outside the culture vessel 1 and slightly protrudes from the upper end of the cylindrical member 7. .
The rotating shaft 4 is made of a thin and deformable wire such as a piano wire, and is formed slightly longer than the coil spring 3. A joint 10 having a diameter larger than the diameter of the coil spring 3 is attached to the upper end of the rotating shaft 4 protruding from the coil spring 3.
Then, after passing the rotating shaft 4 through the coil spring 3, if the lower part of the rotating shaft 4 is bent at an appropriate height from the lower end and inclined at an appropriate angle, the coil spring 3 and the stirring tube outside the rotating shaft 4 are tilted. The lower part of 2 is also inclined. When the upper end portion of the rotating shaft 4 is rotated in this state, the lower end of the rotating shaft 4 and the lower end of the stirring tube 2 covering this rotate so as to draw a circle.
At this time, relative rotation occurs between the rotating shaft 4 rotating around the axis and the stirring tube 2, but the coil spring 3 interposed between the two is connected to the rotating shaft 4. Therefore, a large frictional force is not generated.

図２に示すように、回動軸４の上端部に接続される外部動力としては、上蓋５に着脱自在に設置されるモータ１１を用いることができる。
モータ１１は支持台１２の上面に固定され、その出力軸１３は減速器等を介して支持台１２の下方に突出している。モータ１１は、着脱自在なキャップ１４で被覆される。
また、支持台１２の下面からは４本の支持脚１５が突出され、支持脚１５を上蓋５の上面に載置することにより、モータ１１が上蓋５の上方に設置されると共に、モータ１１の出力軸１３が回動軸４の上端に装着された継手１０に係合される。 As shown in FIG. 2, as the external power connected to the upper end portion of the rotation shaft 4, a motor 11 that is detachably installed on the upper lid 5 can be used.
The motor 11 is fixed to the upper surface of the support base 12, and its output shaft 13 protrudes below the support base 12 via a speed reducer or the like. The motor 11 is covered with a detachable cap 14.
Further, four support legs 15 protrude from the lower surface of the support base 12, and by placing the support legs 15 on the upper surface of the upper lid 5, the motor 11 is installed above the upper lid 5, and the motor 11 The output shaft 13 is engaged with the joint 10 attached to the upper end of the rotating shaft 4.

培養容器１を適宜場所に設置して培養を行う場合は、先ず、培養容器１へ液体培地を充填し、上蓋５に攪拌チューブ２をはめ付け、攪拌チューブ２内にコイルスプリング３を挿入し、さらにコイルスプリング３内に回動軸４を差し込んで、回動軸４の下部を折り曲げ、この状態で上蓋５を培養容器１の上端に取り付ける。
次いで、上蓋５にモータ１１を設置し、モータ１１の出力軸１３を回動軸４の継手１０に接続する。そして、モータ１１を作動させ、攪拌チューブ２で液体培地を攪拌する。
培養容器１を移動する場合であっても、回動軸４の下端部が折り曲げられた状態で、コイルスプリング３、攪拌チューブ２と密着しているので、移動中に上蓋５からモータ１１が離れない。 When culturing with the culture vessel 1 installed in an appropriate place, first, the culture vessel 1 is filled with a liquid medium, the stirring tube 2 is fitted on the upper lid 5, and the coil spring 3 is inserted into the stirring tube 2. Further, the rotating shaft 4 is inserted into the coil spring 3, the lower portion of the rotating shaft 4 is bent, and the upper lid 5 is attached to the upper end of the culture vessel 1 in this state.
Next, the motor 11 is installed on the upper lid 5, and the output shaft 13 of the motor 11 is connected to the joint 10 of the rotating shaft 4. Then, the motor 11 is operated to stir the liquid medium with the stirring tube 2.
Even when the culture vessel 1 is moved, the motor 11 is separated from the upper lid 5 during the movement because the coil spring 3 and the stirring tube 2 are in close contact with the lower end portion of the rotating shaft 4 being bent. Absent.

なお、攪拌チューブ２の下端部には磁石８を装着してあるので、培養容器１をスターラ台の上に設置して内蔵された回転磁石を作動させると、磁石８が培養容器１の周方向に沿って移動するので、攪拌チューブ２によって液体培地が攪拌される。
勿論、モータ１１等の外部動力で回動軸４を回動させることにより攪拌を行うことができるので、攪拌チューブ２の下端部には磁石８を取り付けなくても良い。 In addition, since the magnet 8 is attached to the lower end portion of the stirring tube 2, when the culture vessel 1 is placed on the stirrer base and the built-in rotating magnet is operated, the magnet 8 is moved in the circumferential direction of the culture vessel 1. Therefore, the liquid medium is stirred by the stirring tube 2.
Of course, since stirring can be performed by rotating the rotating shaft 4 with external power from the motor 11 or the like, the magnet 8 does not have to be attached to the lower end of the stirring tube 2.

本発明の実施例を示す浮遊攪拌培養装置の断面図。Sectional drawing of the floating stirring culture apparatus which shows the Example of this invention. モータ設置時の浮遊攪拌培養装置の要部断面図。Sectional drawing of the principal part of the floating stirring culture apparatus at the time of motor installation.

符号の説明Explanation of symbols

１ 培養容器
２ 攪拌チューブ
３ コイルスプリング
４ 回動軸
５ 上蓋
６ 貫通孔
７ 筒部材
８ 磁石
１０ 継手
１１ モータ
１２ 支持台
１３ 出力軸
１４ キャップ
１５ 支持脚
DESCRIPTION OF SYMBOLS 1 Culture container 2 Stirring tube 3 Coil spring 4 Rotating shaft 5 Top cover 6 Through-hole 7 Cylindrical member 8 Magnet 10 Joint 11 Motor 12 Support stand 13 Output shaft 14 Cap 15 Support leg

Claims (3)

培養容器の内部へ、柔軟素材より成り、下端部が閉鎖された攪拌チューブを吊り下げ、該攪拌チューブ内にコイルスプリングを軸方向に沿って設置し、上端部に接続された外部動力で回動駆動され、且つ、下端部を折り曲げた形状に変形可能な回動軸を前記コイルスプリングの中心に挿通したことを特徴とする浮遊攪拌培養装置。   A stirring tube made of a flexible material and closed at the lower end is suspended inside the culture vessel, a coil spring is installed along the axial direction in the stirring tube, and it is rotated by external power connected to the upper end. A floating agitation culture apparatus, characterized in that a rotating shaft that is driven and can be deformed into a bent shape is inserted through the center of the coil spring. 前記攪拌チューブの下端部に磁石を装着した請求項１に記載の浮遊攪拌培養装置。   The floating stirring culture apparatus according to claim 1, wherein a magnet is attached to a lower end portion of the stirring tube. 前記外部動力が、前記培養容器の上蓋に着脱自在に設置されたモータより成り、該モータの出力軸と前記回動軸の上端部とを連結した請求項１又は２に記載の浮遊攪拌培養装置。   The suspension agitation culture apparatus according to claim 1 or 2, wherein the external power is composed of a motor that is detachably installed on the upper lid of the culture vessel, and the output shaft of the motor and the upper end of the rotating shaft are connected. .
JP2003390956A 2003-11-20 2003-11-20 Suspension stirring culture apparatus Pending JP2005151812A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006340607A (en) * 2005-06-07 2006-12-21 World:Kk Culture vessel with stirring mechanism
CN101397539B (en) * 2008-10-14 2011-10-05 中国人民解放军第三军医大学 Force application apparatus simulating human physiological stress of tissue bionic culture for tissue engineering
JP2012068121A (en) * 2010-09-24 2012-04-05 Toyo Seikan Kaisha Ltd Carrier supporting container and method for using the carrier supporting container
CN108485968A (en) * 2018-04-28 2018-09-04 李晓伟 A kind of novel cytotostatic culture device convenient for observation
EP3623039A1 (en) * 2018-09-13 2020-03-18 Bioprocess Control Sweden AB Agitator unit, system and method
JP2022126198A (en) * 2021-02-18 2022-08-30 中村科学器械工業株式会社 Stirring rod, stirring device, and stirring system
CN115337807A (en) * 2022-08-31 2022-11-15 浙江省建德市正发碳酸钙有限公司 Filter-pressing pulp storage barrel for nano calcium carbonate

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006340607A (en) * 2005-06-07 2006-12-21 World:Kk Culture vessel with stirring mechanism
CN101397539B (en) * 2008-10-14 2011-10-05 中国人民解放军第三军医大学 Force application apparatus simulating human physiological stress of tissue bionic culture for tissue engineering
JP2012068121A (en) * 2010-09-24 2012-04-05 Toyo Seikan Kaisha Ltd Carrier supporting container and method for using the carrier supporting container
CN108485968A (en) * 2018-04-28 2018-09-04 李晓伟 A kind of novel cytotostatic culture device convenient for observation
CN108485968B (en) * 2018-04-28 2021-11-19 刘姣姣 Novel cell stable culture equipment convenient to observe
EP3623039A1 (en) * 2018-09-13 2020-03-18 Bioprocess Control Sweden AB Agitator unit, system and method
JP2022126198A (en) * 2021-02-18 2022-08-30 中村科学器械工業株式会社 Stirring rod, stirring device, and stirring system
JP7257059B2 (en) 2021-02-18 2023-04-13 中村科学器械工業株式会社 Stirring Bars, Stirring Devices and Stirring Systems
CN115337807A (en) * 2022-08-31 2022-11-15 浙江省建德市正发碳酸钙有限公司 Filter-pressing pulp storage barrel for nano calcium carbonate

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