JP2005106537A - Sampled liquid chromatograph unit - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a sampled liquid chromatograph unit for performing normal sampling without malfunction, even if measurement under a plurality of analysis conditions is made alternately by a detection section. <P>SOLUTION: Mass scanning is made by two different detection modes (molecule ion detection mode, fragment ion detection mode) (S2, S6), chromatogram data A(0) and A(1) are calculated for each detection mode, based on the acquired mass spectrum data (S4, S8), and A(0) and A(1) are added to acquire total chromatogram data At (S9). A fraction collector executes the control of sampling based on the total chromatogram data At. Accordingly, even if the difference between the data A(0) and A(1) is large, the fraction collector can stably execute the control of sampling since the difference is averaged in the total chromatogram data At. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a preparative liquid chromatograph apparatus for fractionating and collecting a sample separated in a time direction by a liquid chromatograph.

  A so-called preparative liquid chromatograph apparatus that separates and collects a plurality of components contained in a sample using a liquid chromatograph apparatus such as a high performance liquid chromatograph (HPLC) is known. Such a preparative liquid chromatograph is described in Patent Document 1, for example.

Japanese Patent Laid-Open No. 5-302918 ([0002], FIG. 1)

  FIG. 1 is an example of a configuration of a preparative chromatograph using HPLC. The eluent (mobile phase) stored in the eluent tank 11 is sucked by the pump 12 and flows to the column 14 through the sample introduction unit 13 at a constant flow rate. The sample solution injected into the mobile phase in the sample introduction unit 13 rides on the mobile phase, is introduced into the column 14, and is separated and eluted in the time direction while passing through the column 14. The detector 15 sequentially detects components eluted from the column 14 and sends a detection signal to the signal processing unit 16. All or part of the eluate that has passed through the detector 15 is introduced into the fraction collector 18. The signal processing unit 16 creates a chromatogram based on the detection signal obtained from the detector 15, and the control unit 17 provides a control signal for fractionation to the fraction collector 18 in real time based on the peak appearing in the chromatogram. . The fraction collector 18 controls a solenoid valve or the like based on the control signal, and fractionates the eluate into different vials for each component.

  A preparative liquid chromatograph mass spectrometer (preparative LC / MS) using a mass spectrometer (MS) as the detector is widely used. In MS, various components contained in an introduced sample can be separated and detected for each mass number (mass / charge). A mass spectrum is obtained by mass-scanning the set mass range and sequentially detecting the ion intensity separated for each mass number. A total ion chromatogram can be obtained by repeating this mass scan, integrating the mass spectrum of the entire mass scan range for each scan, and taking the change over time. Alternatively, a mass chromatogram can be obtained by paying attention to a specific mass number and taking a time change in ion intensity at that mass number. Using the total ion chromatogram or the mass chromatogram thus obtained, fractionation is performed in the same manner as described above. By using such preparative LC / MS, it is possible to specify the components of the sample collected at the same time as the fractionation.

Usually, in LC / MS, soft ionization methods such as electron spray method and atmospheric pressure chemical ionization method are used. Therefore, unlike electron ionization method (EI method) in gas chromatogram mass spectrometer, proton or solvent is used for molecule M. A simple mass spectrum is obtained in which only ions such as [M + H] ⁺ and [M + Na] ⁺ to which salts (Na, etc.) are added are measured. Hereinafter, a detection mode for performing such detection is referred to as a “molecular ion detection mode”. Furthermore, in LC / MS, in order to obtain molecular structure information, the generated molecular ions are cleaved in the intermediate chamber between the atomization chamber where the molecular ions are generated and the detector (collision-induced cleavage, CID) and the fragment ions generated thereby are detected. Hereinafter, this detection mode is referred to as “fragment ion detection mode”. The cleavage of molecular ions in the intermediate chamber is performed by providing an electrode in the intermediate chamber and changing the voltage applied to this electrode.

  In order to perform the measurement in the molecular ion detection mode and the measurement in the fragment ion detection mode in parallel during one sort operation, the voltage applied to the electrodes in the intermediate chamber is usually controlled to adjust the molecular ion detection mode. The mass scanning by, and the mass scanning by the fragment ion detection mode are alternately performed. Based on such mass scanning, as shown in FIG. 2A, a chromatogram in the molecular ion detection mode and a chromatogram in the fragment ion detection mode are obtained.

  In addition, when multiple mass numbers are specified in SIM (Selected Ion Mass-spectroscopy) mode, which monitors only the specified mass number and creates chromatogram data, measurements at each mass number are executed alternately. The In this case, a chromatogram for each mass number is obtained as shown in FIG.

When the fraction collector is controlled as described above using such a chromatogram, the following problems occur.
The control unit transmits a control signal for fractionation to the fraction collector based on the peak appearing in the chromatogram as described above, and must be able to detect the peak appropriately at that time. In particular, in the fractionation, since the arrival start time and end time of the sample to the fraction collector correspond to the start point and end point of the chromatogram peak, the start point and end point are accurately detected in real time. There must be.

  When the measurement in the molecular ion detection mode and the measurement in the fragment ion detection mode are performed alternately, the data in the chromatogram is obtained alternately in the molecular ion detection mode and in the fragment ion detection mode. However, since the background level and the like are generally different between the molecular ion detection mode and the fragment ion detection mode, the chromatogram data obtained in real time repeatedly increases and decreases at each point as shown in FIG. 2 (b). . When the control unit receives such data, the control unit may erroneously determine that the peak has started or ended at a time other than the peak of the chromatogram. Therefore, fractionation cannot be performed using chromatogram data obtained by alternately performing measurement in such molecular ion detection mode and fragment ion detection mode, and fractionation is performed based on measurement under a single analysis condition. Alternatively, after performing the fractionation based on the measurement under the single analysis condition, it is necessary to perform the fractionation again based on the measurement under the other single analysis condition.

  When detection in the detection mode for each mass number is alternately performed in the SIM measurement mode, data of mass chromatograms for each mass number are sequentially obtained. At this time, the mass chromatogram peaks for each mass number are usually obtained at different times. Therefore, at the time when the mass chromatogram peak of one mass number exists, the peak of the mass chromatogram of another mass number exists. Does not exist. Therefore, the chromatogram data received by the fraction collector is the value of the higher chromatogram data with the peak and the chromatogram data with no peak at the time of the peak of the mass chromatogram for each mass number. The low value is repeated, and sudden increase and decrease are repeated for each point as shown in FIG.

  In either case, since the chromatogram data received by the fraction collector repeats abrupt increase and decrease for each point, the control unit determines that the peak has been started or ended by mistake at times other than the peak of the chromatogram. There is a risk. For this reason, fractionation is performed using chromatogram data obtained by alternately performing the measurement in the molecular ion detection mode and the fragment ion detection mode, and chromatogram data obtained by performing the detection in the detection mode for each mass number in the SIM measurement mode. Cannot be performed, and fractionation is performed based on measurement based on a single analysis condition, or fractionation is performed based on measurement based on a single analysis condition, and then measurement is performed again on another single analysis condition. It was necessary to make a preparative basis.

  Problems to be solved by the present invention include chromatogram data obtained by alternately performing measurement in the molecular ion detection mode and fragment ion detection mode, and chromatogram data obtained by performing detection in the detection mode for each mass number alternately in the SIM measurement mode. Is used to provide a preparative liquid chromatogram apparatus capable of performing normal fractionation without malfunctioning.

The first aspect of the preparative liquid chromatograph apparatus according to the present invention for solving the above-described problems is that a sample obtained by separating components in the time direction in the liquid chromatograph section is used as a mass spectrometer section and a fraction collector. Introducing a liquid chromatograph mass spectrometer that fractionates and collects each component with a fraction collector based on analysis information from the mass analyzer,
a) setting means for presetting analysis conditions for mass spectrometry;
b) Analysis in which the molecular ion detection mode in which the mass spectrum is measured under conditions where molecular ions are detected and the analysis in the fragment ion detection mode in which a fragment of the molecular ion is generated and the mass spectrum is measured are alternately performed. Execution means;
c) Chromatogram data is obtained from the mass spectrum data obtained in one analysis, and the chromatogram obtained by each analysis when the analysis in the molecular ion detection mode and the analysis in the fragment ion detection mode are executed once. Arithmetic means for adding data;
d) a fractionation control means for controlling the operation of the fraction collector based on the chromatogram data obtained by the computing means;
It is characterized by providing.

In the second aspect of the preparative liquid chromatograph apparatus according to the present invention, the sample separated in the time direction by the liquid chromatograph is introduced into the mass analyzer and the fraction collector, and the analysis information by the mass analyzer is used as analysis information. In a liquid chromatograph mass spectrometer that fractionates and collects each component with a fraction collector,
a) setting means for presetting analysis conditions for mass spectrometry;
b) Analyzing means for measuring mass spectrum data at a specific mass number and sequentially executing the measurement for a plurality of mass numbers;
c) Chromatogram data at a specific mass number is obtained from the mass spectrum data obtained in one analysis, and chromatogram data obtained by each analysis when the analysis for the plurality of mass numbers is performed once. Computing means for adding
It is characterized by providing.

Embodiments and effects of the invention

  In the preparative liquid chromatograph apparatus of the first aspect, the measurer presets the analysis conditions in the mass spectrometer by the setting means. When the setting means sets the molecular ion detection mode and the fragment ion detection mode, the analysis execution means alternately executes the analysis in the molecular ion detection mode and the analysis in the fragment ion detection mode. By mass scanning in each detection mode, mass spectrum data for a predetermined range of mass numbers is obtained. The calculation means adds the mass spectrum data for each of these two detection modes to obtain chromatogram data. Further, the chromatogram data of the two detection modes are added to obtain one chromatogram data. Since this single chromatogram data reflects the mass spectrum of the molecular ion detection mode and the fragment ion detection mode, they are averaged even if the background levels of the two measurement modes are different. .

  In the preparative liquid chromatograph apparatus according to the second aspect, as in the case of the first aspect, the measurer presets the analysis conditions in the mass spectrometer by the setting means. When the setting means is set so that the measurement in the SIM measurement mode is performed for a plurality of mass numbers, the analysis execution means sequentially measures the mass spectrum for each set mass number. The computing means obtains chromatogram data for each mass number from the obtained mass spectrum. Further, the chromatogram data for each mass number is added to obtain one chromatogram data. Since this single chromatogram data reflects mass spectra of a plurality of mass numbers, even if the presence or absence of a peak differs for each mass number, they are averaged.

  In addition, when the molecular ion detection mode and the fragment ion detection mode are alternately executed as described above, mass scanning is normally alternately performed once for each measurement mode in order to execute a plurality of analysis conditions in a short time. In order to increase the analysis accuracy, it may be alternately performed a plurality of times. Similarly, in the measurement in the SIM mode, mass scanning may be alternately performed a plurality of times for each mass number.

  According to the preparative liquid chromatograph apparatus of the first aspect, even when the molecular ion detection mode and the fragment ion detection mode are executed alternately, the peak waveform is normal, unlike the waveform that repeats sudden increase and decrease as in the prior art. The chromatogram data as obtained in the above can be acquired. Similarly, according to the preparative liquid chromatograph device of the second aspect, when the measurement in the SIM measurement mode is sequentially performed on a plurality of mass numbers, the same effect as that of the first aspect is obtained. Can be obtained. By using such chromatogram data, each component can be appropriately separated by a fraction collector. In addition, since molecular ion detection mode and fragment ion detection mode, or SIM measurement for multiple mass numbers can be performed in one analysis, fractionation can be performed based on measurement under a single analysis condition, It is not necessary to perform fractionation again based on measurement under another single analysis condition after performing fractionation based on the measurement under the analysis conditions, and the time required for measurement and fractionation can be shortened.

  The schematic block diagram of one Example of the preparative chromatograph apparatus of this invention is shown in FIG. This preparative chromatograph is a preparative LC / MS using MS in the analysis part, and uses an atmospheric pressure chemical ionization method (APCI). The sample solution eluted from the LC column 14 is branched into two flow paths at a predetermined branching ratio in the flow path branching section 19, one is sent to the MS section 20, and the other is sent to the fraction collector 40. The MS unit 20 includes an atomization chamber 21 in which a nozzle 22 and a discharge electrode 23 are disposed, and an analysis chamber 26 in which a quadrupole filter 28 and an ion detector 29 are disposed. Two intermediate chambers 25 are provided between the chambers 26. The atomization chamber 21 and the first-stage intermediate chamber 25 communicate with each other through a desolvation tube 24. The intermediate chamber 25 is provided with an intermediate electrode 27 capable of accelerating ions and generating fragment ions by controlling the applied voltage. The detection signal of the ion detector 29 is input to the signal processing unit 31, and the signal processing unit 31 performs processing as described below. The control unit 32 controls the operation of each unit of the MS unit 20 and each unit of the LC and also controls the operation of the fraction collector 40. The fraction collector 40 includes an electromagnetic valve 41 and performs fractionation based on a control signal from the control unit 32.

  The operation of the MS unit 20 will be described. When the sample solution supplied from the column 14 reaches the nozzle 22, it is sprayed into the atomization chamber 21 as high-temperature droplets. The ejected droplet collides with gas molecules at atmospheric pressure, and is further pulverized into fine droplets, dried quickly (desolvated), and sample molecules are vaporized. The gaseous fine particles come into contact with buffer ions generated by corona discharge from the discharge electrode 23 and are ionized by causing a chemical reaction. The fine droplets containing the generated ions jump into the desolvation tube 24 and further desolvation proceeds while passing through the desolvation tube 24. The ions are sent to the analysis chamber 26 through the two intermediate chambers 25 while being accelerated by the voltage applied to the intermediate electrode 27. Here, when the analysis is performed in the molecular ion detection mode, the molecular ions generated in the atomization chamber 21 are sent to the analysis chamber 26 as they are, whereas when the analysis is performed in the fragment ion detection mode, By controlling the voltage applied to the intermediate electrode 27, the molecular ions are collision-induced cleaved to generate fragment ions. In any detection mode, by controlling the voltage applied to the quadrupole filter 28, the mass number (mass / charge) of ions passing through the quadrupole filter 28 and reaching the ion detector 29 is scanned. To do. In the ion detector 29, a current corresponding to the number of reached ions is taken out. Switching between the molecular ion detection mode and the fragment ion detection mode can be performed in a short time by changing the voltage applied to the intermediate electrode 27, the discharge electrode 23, etc., and switching the operation of the ion detector 29.

Hereinafter, the operation of this LC / MS in the case of performing a sorting operation with switching between the molecular ion detection mode and the fragment ion detection mode will be described.
First, the operator inputs various parameters such as LC operation conditions, MS unit 20 operation conditions, and signal processing unit 31 processing conditions from the operation unit 33. FIG. 5 shows a window displayed on the screen of the operation unit 33 for inputting mass spectrum measurement conditions among these various parameters. When a sorting operation involving switching of a plurality of analysis conditions is performed, the same analysis start time and analysis end time are input to a plurality of rows in the analysis start / end time input unit 51. In the example of FIG. 5, since the same “analysis start time = 0 minutes, analysis end time = 10 minutes” is input to the first and second lines of the analysis start / end time input unit 51, two analysis conditions are set. It shows that the sorting operation is performed while switching. Furthermore, after clicking each row of the analysis start / end time input unit 51 and inputting each parameter of the mass spectrum measurement condition input unit 52, the mass spectrum measurement condition is set for each row of the analysis start / end time input unit 51. Is set. Here, by appropriately setting the input values of the probe voltage input unit 53 and the CDL voltage input unit 54, one of the two analysis conditions is set to the molecular ion detection mode and the other is set to the fragment ion detection mode.

  Further, when the display is switched by clicking the tab 55 of the window in FIG. 5, a window for inputting conditions for creating chromatogram data is displayed as shown in FIG. Here, based on the mass spectrum measurement conditions input in the window of FIG. 5, the mass number range in which the mass spectrum is integrated is input. Here, “TIC” input to the integration type input unit 56 means that integration is performed over the entire measurement mass range of the mass spectrum, and “MIC” is the mass number range input to the mass number range input unit 57. It means to perform the integration. Thereby, the input of a plurality of different analysis conditions (two in the examples of FIGS. 5 and 6) is completed.

  When the measurer performs a predetermined operation, the sample is introduced from the sample introduction unit 13 into the LC. This sample is introduced into the MS unit 20, and analysis by the MS unit 20 is performed according to the analysis conditions set above. Here, operation | movement of the signal processing part 31 and the control part 32 is demonstrated using the flowchart of FIG.

  First, the control unit 32 sets parameters of each unit of the MS unit 20 so as to be in the molecular ion detection mode (step S1), and executes mass scanning in a predetermined mass range (step S2). During mass scanning, when the applied voltage to the quadrupole filter 28 is scanned, the mass number of ions that pass through the quadrupole filter 28 and reach the ion detector 29 changes. The signal processing unit 31 processes detection signals that sequentially change during mass scanning, and acquires mass spectrum data indicating the relationship between the mass number and the ion intensity (step S3). Among the mass spectrum data, mass spectrum data is extracted according to the preset processing conditions (mass range information), and these are integrated to calculate the molecular ion detection mode chromatogram data A (0). Store (step S4).

  Next, the control unit 32 sets parameters of each unit of the MS unit 20 so as to be in the fragment ion detection mode (step S5), and executes mass scanning in a predetermined mass range (step S6). That is, mass scanning is executed in the same manner as in the molecular ion detection mode, and the signal processing unit 31 processes detection signals that sequentially change during mass scanning, and obtains mass spectrum data indicating the relationship between mass number and ion intensity. (Step S7). Among the mass spectrum data, mass spectrum data is extracted according to preset processing conditions, and these are integrated to calculate fragment ion detection mode chromatogram data A (1) (step S8).

  The signal processing unit 31 adds the chromatogram data A (0) and A (1) to determine the combined chromatogram data At (step S9). Then, based on the combined chromatogram data At, the control unit 32 controls the fraction collector 40 (step S10). Details of this control will be described later. If the predetermined analysis time has not elapsed, the process returns from step S11 to S1, and the above-described processing is continued.

  By the above processing, as shown in FIG. 8, one chromatogram data A is obtained for every two mass scans under different analysis conditions. FIG. 9 shows an example of temporal changes in the chromatogram data A (0), A (1) and the combined chromatogram data At obtained by the above processing. Even if the intensity of the two chromatogram data A (0) and A (1) are different as shown in FIG. 9 (a), the combined chromatogram data At is one normal as shown in FIG. 9 (b). A peak is shown, and there is no sudden increase or decrease in chromatogram data as shown in FIG. Therefore, the fraction collector 40 can correctly perform sorting without malfunction due to sudden increase / decrease in chromatogram data signal.

  The control of the fraction collector 40 by the control unit 32 is performed as follows. When the control unit 32 receives the combined chromatogram data At from the signal processing unit 31 in real time, the control unit 32 detects the peak start point of the combined chromatogram data At according to a predetermined standard. After a predetermined delay time has elapsed since the start point was detected, a sampling start signal is transmitted to the fraction collector 40. Here, the delay time is determined according to the flow rate of the mobile phase, the pipe capacity from the flow path branching section 19 to the nozzle 22 of the MS section 20, the piping capacity from the flow path branching section 19 to the electromagnetic valve 41, and the like. The fraction collector 40 that has received the sorting start signal opens the solenoid valve 41 and starts sample sorting. Similarly, when the control unit 32 detects the end point of the peak of the combined chromatogram data At, the control unit 32 transmits a fractionation end signal to the fraction collector 40 after a predetermined delay time has elapsed. This completes the sorting for one component. When dispensing multiple components, the vial is moved by a biaxial arm or the like while the solenoid valve is closed, and an empty vial is set at the sorting position to prepare for the next sorting. Complete.

  If the analysis is performed under a plurality of analysis conditions and only the chromatogram obtained from the single analysis condition is used for the operation of the fraction collector, the output control check 58 in FIG. 6 is removed. Thus, analysis conditions may be created under conditions that are not used for chromatogram addition, and during the analysis process, one of chromatogram data A (0) or A (1) may be set to 0.

  Next, the operation of this LC / MS when performing the measurement in the SIM measurement mode for a plurality of mass numbers will be described using the flowchart of FIG. Here, SIM measurement mode measurement is performed for n types (n is an integer of 2 or more) mass numbers m (k) = m (1), m (2), ..., m (n) Will be described. In this measurement, either the molecular ion detection mode or the fragment ion detection mode is used. These modes are set by the measurer from the operation unit 33 before the analysis is started, and the control unit 32 sets parameters of each unit of the MS unit 20 based on the mode.

  First, the control unit 32 sets the parameter k to 1 (step S21), and acquires mass spectrum data at the mass number m (1) (step S22). The signal processing unit 31 calculates chromatogram data A (1) from the mass spectrum data and stores it in the memory (step S23). Next, in step S24, it is checked whether or not the parameter k is larger than n. If k <n, 1 is added to k (step S25), and the operations in steps S22 to S24 are repeated. If k is greater than or equal to n, the measurement for n kinds of mass numbers has been completed, and the process proceeds to the next step S26. In this step S26, the chromatogram data A (1), A (2),..., A (n) for the obtained n kinds of mass numbers are summed to obtain summed chromatogram data At. Then, based on the total chromatogram data At, the control unit 32 controls the fraction collector 40 (step S27). The fraction collector 40 is controlled in the same manner as described above. If the predetermined analysis time has not elapsed, the process returns from step S27 to S21, and the above-described processing is continued.

  FIG. 11 shows an example of temporal changes in the chromatogram data and the combined chromatogram data At obtained in the SIM measurement mode of this embodiment. In this figure, the case where the measurement in the SIM measurement mode is performed for two kinds of mass numbers is taken as an example. As shown in (a), even if the two chromatogram data A (1) and A (2) have peaks at two different positions, the combined chromatogram data At is Normal peaks are shown at each of the two peak positions, and there is no sudden increase or decrease in chromatogram data. Therefore, the fraction collector 40 can correctly perform sorting without malfunction due to sudden increase / decrease in chromatogram data signal.

The schematic block diagram which shows an example of the conventional preparative liquid chromatograph apparatus. The graph for demonstrating the problem of the signal processing in the conventional preparative liquid chromatograph apparatus. The graph for demonstrating the problem of the signal processing in the conventional preparative liquid chromatograph apparatus. The schematic block diagram which shows one Example of the preparative chromatograph apparatus which concerns on this invention. The figure showing the window for inputting mass spectrum measurement conditions. The figure showing the window for inputting the conditions which create chromatogram data. The flowchart which shows operation | movement in the case of performing molecular ion detection mode and fragment ion detection mode alternately. The schematic diagram for demonstrating the signal processing operation | movement of a present Example. The graph showing an example of the data of the chromatogram obtained in a present Example when performing molecular ion detection mode and fragment ion detection mode alternately. The flowchart which shows operation | movement in the case of performing the measurement of SIM measurement mode with respect to several mass numbers. The graph showing an example of the data of the chromatogram obtained by a present Example, when performing the measurement of SIM measurement mode with respect to several mass numbers.

Explanation of symbols

DESCRIPTION OF SYMBOLS 11 ... Eluent tank 12 ... Pump 13 ... Sample introduction part 14 ... Column 15 ... Detector 16 ... Signal processing part 17 ... Control part 18 ... Fraction collector 19 ... Flow path branching part 20 ... MS part 21 ... Atomization chamber 22 ... Nozzle 23 ... Discharge electrode 24 ... Desolvation tube 25 ... Intermediate chamber 26 ... Analysis chamber 27 ... Intermediate electrode 28 ... Quadrupole filter 29 ... Ion detector 31 ... Signal processing unit 32 ... Control unit 33 ... Operation unit 40 ... Fraction collector 41 ... Solenoid valve 51 ... Analysis start / end time input unit 52 ... Mass spectrum measurement condition input unit 53 ... Probe voltage input unit 54 ... CDL voltage input unit 55 ... Tab 56 ... Integration type input unit 57 ... Mass number range input unit 58 ... Output control check

Claims (2)

A liquid chromatograph mass spectrometer that samples a component separated in the time direction in the liquid chromatograph unit into the mass analyzer and fraction collector, and fractionates and collects each component based on the analysis information from the mass analyzer In
a) setting means for presetting analysis conditions for mass spectrometry;
b) Analysis in which the molecular ion detection mode in which the mass spectrum is measured under conditions where molecular ions are detected and the analysis in the fragment ion detection mode in which a fragment of the molecular ion is generated and the mass spectrum is measured are alternately performed. Execution means;
c) Chromatogram data is obtained from the mass spectrum data obtained in one analysis, and the chromatogram obtained by each analysis when the analysis in the molecular ion detection mode and the analysis in the fragment ion detection mode are executed once. Arithmetic means for adding data;
d) a fractionation control means for controlling the operation of the fraction collector based on the chromatogram data obtained by the computing means;
A liquid chromatograph mass spectrometer comprising: A liquid chromatograph mass spectrometer that samples a component separated in the time direction in the liquid chromatograph unit into the mass analyzer and fraction collector, and fractionates and collects each component based on the analysis information from the mass analyzer In
a) setting means for presetting analysis conditions for mass spectrometry;
b) analyzing execution means for measuring data of a mass spectrum at a specific mass number and sequentially executing the measurement for a plurality of mass numbers;
c) Chromatogram data at a specific mass number is obtained from the mass spectrum data obtained in one analysis, and chromatogram data obtained by each analysis when the analysis for the plurality of mass numbers is performed once. Computing means for adding
A liquid chromatograph mass spectrometer comprising:

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