JP2005095159A - Extraction apparatus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To efficiently extract a specific substance such as nucleic acid and the like in a sample solution in short time and enhance reliability by homogeneously introducing pressurized air to an extraction cartridge. <P>SOLUTION: The specific material in a sample solution is adsorbed by using extraction cartridges 11 installed with a filter member. A pressurized air-supply mechanism 4 is installed with an air pump 43 and a pressure sensor 46 detecting the inner pressure of the cartridges, introduces the pressurized air to the extraction cartridges and acts a predetermined pressure to the extraction cartridges 11 by tightly closing the cartridges when the inner pressure reaches the range of the predetermined pressure. A case where the pressurized air is introduced to the cartridges in order and a case where pressurised airs are introduced thereto simultaneously are involved. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an extraction apparatus for extracting a specific substance such as a nucleic acid in a sample solution using an extraction cartridge having a filter member, and more particularly to a mechanism for introducing pressurized air into the extraction cartridge.

  Conventional extraction methods, for example, nucleic acid extraction methods include those by centrifugation, those using magnetic beads, and those using filters.

  As an extraction device using a filter, a large number of filter tubes containing a filter are set in a rack, and sample liquid is dispensed into the rack, and the periphery of the bottom of the rack is sealed with an air chamber through a sealing material. The sample solution is sucked from the discharge side at the same time to allow the sample solution to pass through, and the nucleic acid is adsorbed to the filter. After that, the washing solution and the eluate are dispensed, and similarly, the solution is sucked under reduced pressure to be washed and eluted. Such a mechanism has been proposed (see, for example, Patent Document 1).

Another extraction method using a filter is a method in which a sample solution is injected into a separation and purification unit equipped with a specific filter that adsorbs the nucleic acid of the sample solution and then is separated and recovered, and then extracted by pressurization. Some have been adopted (see, for example, Patent Document 2).
Japanese Patent No. 2832586 JP 2003-128691 A

  In what was described in patent documents 1 and 2 as mentioned above, there exists the following problems as an extraction apparatus made into the object of this invention.

  Cited Document 1 shows a diffusion extraction device by a decompression method, and the device that sucks the whole at the same time, because the decompression volume is large, the device becomes large and takes time until the decompression is applied. It is difficult to detect the discharge, and the time setting is long, which is an obstacle to improving the processing efficiency. In particular, in the whole vacuum suction, if there is no air resistance due to poor sample liquid injection into one extraction cartridge, misloading of the extraction cartridge, etc., normal operation cannot be ensured, and many It is also difficult to configure a mechanism for individually sucking the extraction cartridge.

  On the other hand, Patent Document 2 shows a method of adsorbing and recovering a nucleic acid extract using a filter by a pressurization method, but a specific extraction device is not shown. In the extraction apparatus adopting the above, it is expected that the pressurization control method, the generation of contamination due to the scattering of the discharged liquid during pressurization, the securing of the sealing property, etc. will be problems.

  In particular, when the pressurized air is sequentially introduced into the extraction cartridge, the pressurized air is not evenly introduced into the extraction cartridge, or the pressure change due to the operation of the open / close valve individually installed for each extraction cartridge is large. As a result, the detection accuracy of the pressure sensor becomes unstable, and it becomes difficult to ensure control reliability.

  The present invention has been made in view of these points, and provides an extraction apparatus that can efficiently extract a specific substance such as nucleic acid in a sample solution in a short time and can introduce pressurized air evenly into an extraction cartridge. It is intended to do.

  In particular, the present invention provides an extraction apparatus that can satisfactorily realize nucleic acid extraction by the filter method of Patent Document 2.

  The extraction device of the present invention uses an extraction cartridge provided with a filter member, injects a sample liquid containing a specific substance into the extraction cartridge, and adsorbs the specific substance in the sample liquid to the filter member. A pressurized air supply mechanism that introduces pressurized air into the cartridge includes an air pump and a pressure sensor that detects an internal pressure of the extraction cartridge. The pressurized air is introduced into the extraction cartridge, and the internal pressure reaches a predetermined pressure range. In this case, the extraction cartridge is sealed and a predetermined pressure is applied to the extraction cartridge.

  It is particularly preferred that there are a plurality of extraction cartridges, and the pressure sensor individually detects the internal pressure of each extraction cartridge.

  Another extraction apparatus of the present invention uses an extraction cartridge provided with a filter member, injects a sample liquid containing nucleic acid into the extraction cartridge and pressurizes the nucleic acid in the sample liquid to be adsorbed on the filter member, In the extraction device that dispenses and pressurizes the recovery liquid into the extraction cartridge and separates the nucleic acid adsorbed on the filter member and recovers it together with the recovery liquid, a pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes: An air pump, an open / close valve that opens and closes introduction of pressurized air into the extraction cartridge, and a pressure sensor that detects an internal pressure of the extraction cartridge, and opens the open / close valve to supply pressurized air to the corresponding extraction cartridge. When the internal pressure reaches a predetermined pressure range, the open / close valve is closed and sealed in a pressurized state, and a predetermined pressure is applied to the extraction cartridge. It is characterized in that to use.

  At that time, one of the open / close valves that are in the closed state is opened, pressurized air is introduced into the corresponding extraction cartridge, and the open / close valve is closed according to the detection of the pressure sensor. Subsequently, the operation of opening the next one opening / closing valve, introducing the pressurized air into the corresponding next extraction cartridge, and repeating the operation of closing the opening / closing valve according to the detection of the pressure sensor, It is preferable to introduce pressurized air into the extraction cartridge when the state of the extraction cartridge, the viscosity of the liquid, etc. are unknown.

  In addition, a plurality of open / close valves are opened among all the open / close valves that are closed, and pressurized air is simultaneously introduced into the corresponding extraction cartridges, and the open / close valves are closed according to the detection of the pressure sensors. Then, it is preferable to introduce the pressurized air into the plurality of extraction cartridges in terms of increasing the processing efficiency.

  At this time, it is preferable that the discharge rate of the air pump is controlled according to the number of the open / close valves that are opened to make the pressure increase rate constant.

  On the other hand, after the pressurized air introduced into the extraction cartridge is sealed by closing the open / close valve, the pressure end point when the liquid discharge is completed through the filter member in the extraction cartridge is determined by the pressure sensor when the liquid discharge is completed. It is preferable to individually detect the internal pressure drop and shift to the subsequent steps.

  Further, another extraction apparatus of the present invention uses an extraction cartridge provided with a filter member, injects a sample liquid containing nucleic acid into the extraction cartridge and pressurizes it, and adsorbs the nucleic acid in the sample liquid to the filter member. Then, the extraction liquid is dispensed into the extraction cartridge and pressurized to separate the nucleic acid adsorbed on the filter member and recovered together with the recovery liquid. The pressurized air supply mechanism to be introduced includes an air pump and a mechanical open / close valve that individually opens and closes the introduction of the pressurized air to the extraction cartridge, and the mechanical open / close valve is closed at an upper limit set pressure for an upper limit of pressurization. It operates to open at the lower limit set pressure, and when it reaches the upper limit set pressure, it introduces pressurized air to the corresponding extraction cartridge in the open state. Pressure is applied to the sealed with pressure state infusate, is characterized in that the opening operation when the liquid ejection completion time of the pressure drop from the extraction cartridge.

  Another extraction apparatus of the present invention uses an extraction cartridge provided with a filter member, injects a sample liquid containing nucleic acid into the extraction cartridge and pressurizes the nucleic acid in the sample liquid to be adsorbed on the filter member, In the extraction device that dispenses and pressurizes the recovery liquid into the extraction cartridge and separates the nucleic acid adsorbed on the filter member and recovers it together with the recovery liquid, a pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes: One air pump, an open / close valve that opens and closes the introduction of pressurized air to the extraction cartridge, a pressure sensor that detects the internal pressure of the extraction cartridge, and the pressure of the air passage between the air pump and the open / close valve is released to the atmosphere A relief valve that operates, introduces pressurized air into the extraction cartridge and seals it, and detects the extraction cart by detecting the pressure sensor. When the liquid is discharged from the wedge, the opening / closing valve is opened and the relief valve is opened to the atmosphere, and the pressurized air remaining in the extraction cartridge is released to the atmosphere via the opening / closing valve and the relief valve. It is characterized by doing.

  Still another extraction apparatus of the present invention uses an extraction cartridge provided with a filter member, and injects a sample liquid containing nucleic acid into the extraction cartridge and pressurizes it to adsorb the nucleic acid in the sample liquid to the filter member. In the extraction device that dispenses and pressurizes the recovery liquid into the extraction cartridge and separates the nucleic acid adsorbed on the filter member and recovers it together with the recovery liquid, a pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes: One air pump, an open / close valve that individually opens and closes the introduction of pressurized air to each extraction cartridge, a pressure sensor that individually detects the internal pressure of each extraction cartridge, and between the air pump and the open / close valve A relief valve for releasing the pressure of the air passage to the atmosphere, and opening one of the open / close valves to pressurize air to the corresponding extraction cartridge. After the supply, the open / close valve is closed, and when all the open / close valves are in the closed state, the relief valve is opened to the atmosphere, and the excessively pressurized air accompanying the continued driving of the air pump is passed through the relief valve. It is characterized by opening to the atmosphere.

  According to the present invention as described above, an extraction operation in which a specific substance, for example, a sample solution containing nucleic acid is injected into an extraction cartridge provided with a filter member and pressurized to adsorb the specific substance to the filter member is efficiently performed in a short time. Thus, a mechanism capable of extracting a specific substance such as a nucleic acid in a sample solution can be configured in a compact manner.

  The pressurized air supply mechanism further includes an air pump and a pressure sensor for detecting internal pressure, introduces pressurized air to the extraction cartridge, and seals the extraction cartridge in a pressurized state when the internal pressure reaches a predetermined pressure range. By applying a predetermined pressure to the air, pressurized air can be uniformly introduced into each extraction cartridge, and a specific substance such as a nucleic acid can be stably extracted, thereby improving reliability.

  At that time, in the case where the open / close valves that are individually opened and closed are sequentially opened and closed, and the pressurized air is introduced into the extraction cartridge one by one, there is a place where the extraction cartridge is not set or the extraction in which no liquid is injected Even when there is a cartridge or when the viscosity of the liquid is extremely different, each extraction cartridge can be individually controlled to open and close, and uniform pressure can be introduced.

  In addition, when a plurality of open / close valves are opened and pressurized air is simultaneously introduced into each extraction cartridge, the processing time by simultaneous pressurization can be shortened. In this case, if the discharge amount of the air pump is controlled according to the number of opening / closing valves that are opened, the pressure increase rate can be kept constant regardless of the number of extraction cartridges to be pressurized at the same time.

  On the other hand, when the end of pressurization in the extraction cartridge is detected by the pressure sensor from the decrease in internal pressure when the liquid discharge is completed, the process can be shifted to the subsequent process according to the detection, and the processing efficiency can be increased.

  Further, in the other extraction device of the present invention, an internal pressure is detected by including one air pump and a separately installed mechanical opening / closing valve that is closed at the upper limit set pressure and opened at the lower limit set pressure. This eliminates the need for a pressure sensor to be used and simplifies the mechanism.

  In another extraction apparatus of the present invention, a pressurized air supply mechanism that introduces pressurized air into the extraction cartridge operates to release the pressure of the air passage between the air pump and the open / close valve that opens and closes the introduction of the pressurized air to the atmosphere. An extraction cartridge is provided that has a relief valve and opens the open / close valve when the liquid is completely discharged from the extraction cartridge, and opens the relief valve to the atmosphere to release the pressurized air remaining in the extraction cartridge to the atmosphere. It is possible to prevent the remaining air from being ejected together with the liquid from the discharge portion and to be scattered in the form of mist, and contamination due to this can be prevented.

  In another invention, when opening / closing valves that are individually opened / closed are sequentially opened / closed to introduce pressurized air into the extraction cartridge, one opening / closing valve is opened to supply pressurized air to the corresponding extraction cartridge. When all the open / close valves are closed after the open / close valve is closed, the relief valve is opened to the atmosphere and excessive pressure is applied as the air pump continues to drive. By releasing the air to the atmosphere, the pressurized air introduced into the extraction cartridge is always in an appropriate state, and the occurrence of contamination caused by this can be prevented without causing abnormal ejection of discharged liquid due to an increase in internal pressure.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings. 1 is a perspective view showing a state in which the cover of the nucleic acid extraction apparatus in one embodiment of the extraction apparatus is removed, FIG. 2 is a schematic mechanism diagram of the nucleic acid extraction apparatus, FIG. 3 is a perspective view of a rack in the mounting mechanism, and FIG. Is a perspective view showing the use state of the rack, FIG. 5 is an air system diagram of the pressurized air supply mechanism, FIG. 6 is a diagram showing fluctuations in the pressure in the extraction cartridge, FIG. 7 is an air system diagram of another form, and FIG. FIG. 9 is a perspective view of the extraction cartridge.

  Before describing the mechanism of the nucleic acid extraction apparatus 1 of one embodiment, the nucleic acid extraction apparatus 1 extracts a nucleic acid as a specific substance in a sample solution using an extraction cartridge 11 (filter cartridge) as shown in FIG. To do. In this extraction cartridge 11, a filter member 11b is held at the bottom of a cylindrical main body 11a whose upper end is open, a portion below the filter member 11b of the cylindrical main body 11a is formed in a funnel shape, and a capillary tube-like shape is formed at the center of the lower end. The discharge portion 11c is formed to protrude to a predetermined length, and the vertical projections 11d are formed on both sides of the cylindrical body 11a. Sample liquid, cleaning liquid, and recovery liquid, which will be described later, are dispensed from the upper opening, pressurized air is introduced from the upper opening, and each liquid flows down to the waste liquid container 12 or recovery container 13 to be described later from the discharge portion 11c through the filter member 11b. To do. In the illustrated case, the cylindrical main body 11a is divided into an upper part and a lower part and is fitted.

  The nucleic acid extraction apparatus 1 basically performs nucleic acid extraction and purification by an extraction process as shown in FIGS. First, in step (a) of FIG. 8, the sample solution S containing the dissolved nucleic acid is injected into the extraction cartridge 11 located on the waste liquid container 12. Next, in the step of FIG. 8B, pressurized air is introduced into the extraction cartridge 11 to pressurize it, the sample solution S is passed through the filter member 11b, the nucleic acid is adsorbed to the filter member 11b, and the liquid component that has passed is Discharge into the waste liquid container 12.

  Next, the washing liquid W is automatically dispensed to the extraction cartridge 11 in the step (c) of FIG. 8, and pressurized air is introduced into the extraction cartridge 11 and pressurized in the step (d), while the nucleic acid is retained in the filter member 11b. The impurities W are cleaned and removed, and the cleaning liquid W that has passed is discharged into the waste liquid container 12. This step (c) and step (d) may be repeated a plurality of times.

  Thereafter, the waste liquid container 12 below the extraction cartridge 11 is replaced with the recovery container 13 in the step (e), and the recovery liquid R is automatically dispensed into the extraction cartridge 11 in the step (f), and the extraction is performed in the step (g). Pressurized air is introduced into the cartridge 11 for pressurization, the binding force between the filter member 11b and the nucleic acid is weakened, the adsorbed nucleic acid is released, and the recovery liquid R containing the nucleic acid is discharged into the recovery container 13 and recovered.

  The filter member 11b in the extraction cartridge 11 is basically porous so that the nucleic acid can pass through, and the surface has a characteristic of adsorbing the nucleic acid in the sample solution with a chemical binding force. The adsorption is held, and the nucleic acid adsorption force is weakened and separated at the time of recovery by the recovery liquid. For example, the specific structure of the filter member 11b is composed of an organic polymer having a hydroxyl group on the surface, as described in detail in the method for separating and purifying nucleic acid disclosed in JP-A-2003-128691. The organic polymer having a hydroxyl group on the surface is preferably a surface saponified product of acetylcellulose. As acetyl cellulose, any of monoacetyl cellulose, diacetyl cellulose, and triacetyl cellulose may be used, and triacetyl cellulose is particularly preferable. The surface is saponified by contact with a saponification solution (eg, NaOH), and the structure remains acetylcellulose. The amount (density) of hydroxyl groups on the surface can be controlled by the degree of surface saponification treatment (surface saponification degree), and the higher the number of hydroxyl groups, the higher the nucleic acid adsorption effect. For example, in the case of acetylcellulose such as triacetylcellulose, the surface saponification rate is preferably about 5% or more, and more preferably 10% or more. A porous membrane is suitable for acetylcellulose.

  The “sample solution S containing nucleic acid” is obtained by adding a water-soluble organic solvent to a solution in which nucleic acid is dispersed in a solution by dissolving a specimen containing cells or viruses. For example, in the diagnostic field, whole blood collected as a specimen, plasma, serum, urine, feces, semen, saliva, and other body fluids, plants (or parts thereof), animals (or parts thereof), or the like Of interest are solutions prepared from biological materials such as lysates and homogenates. The “lysis treatment” is a treatment with an aqueous solution containing a reagent (eg, a solution containing a guanidine salt, a surfactant and a proteolytic enzyme) that dissolves cell membranes and nuclear membranes to solubilize nucleic acids. When the resulting sample is whole blood, red blood cells and various proteins are decomposed and depolymerized to prevent non-specific adsorption and clogging to the filter member 11b, and leukocytes and nuclear membranes are solubilized to solubilize the nucleic acid to be extracted. Dissolve. Examples of the “water-soluble organic solvent” include ethanol, isopropanol, propanol and the like, and ethanol is particularly preferable. The concentration of the water-soluble organic solvent is preferably 5 to 90% by weight, more preferably 20 to 60% by weight. The concentration of ethanol added is particularly preferably as high as possible without causing aggregates.

  The “washing liquid W” has a function of washing out impurities in the sample liquid adhering to the filter member 11b together with the nucleic acid, and has a composition for removing the impurities without removing the nucleic acid. It consists of an aqueous solution containing a main agent, a buffering agent, and optionally a surfactant. Examples of the main agent include about 10 to 100% by weight (preferably 20 to 100% by weight, more preferably 40 to 80% by weight) of an aqueous solution of methanol, ethanol, isopropanol, n-isopropanol, butanol, acetone and the like.

  The “recovered liquid R” preferably has a low salt concentration, and in particular, a solution having a salt concentration of 0.5 M or less, such as purified distilled water or TE buffer, is used.

  As shown in FIGS. 1 and 2, the nucleic acid extraction apparatus 1 includes a main body 2, a mounting mechanism 3 that holds a plurality of extraction cartridges 11, a waste liquid container 12, and a collection container 13, and pressurized air in the extraction cartridge 11. And a dispensing mechanism 5 for dispensing the cleaning liquid W and the recovered liquid R to the extraction cartridge 11. Next, each mechanism 3-5 is demonstrated concretely.

<Mounting mechanism>
The mounting mechanism 3 includes a mounting base 21 at the lower front portion of the apparatus main body 2, and a rack 6 holding a plurality of extraction cartridges 11, waste liquid containers 12 and recovery containers 13 is placed on the mounting base 21. As shown in FIG. 3, the rack 6 includes a stand 61, a cartridge holder 62, and a container holder 63.

  The stand 61 holds the cartridge holder 62 on the columnar parts 61a on both sides so as to be movable up and down, and holds the container holder 63 on the bottom plate 61b between the columnar parts 61a so as to be movable back and forth.

  The cartridge holder 62 is configured in a two-part structure by joining front and rear plate materials, and includes support legs 62b extending in the vertical direction at both ends of a holding portion 62a extending in the horizontal direction. The support leg 62b is inserted into the vertical sliding groove 61c of the columnar portion 61a of the stand 61 so as to be vertically movable, and the support leg 62b is biased upward by a biasing member (not shown) built in the stand 61. Has been. A plurality of holding holes 62c are arranged in the holding portion 62a, the extraction cartridge 11 is inserted from above, and the lower ends of protrusions 11d (see FIG. 9) formed on both sides of the cylindrical body 11a of the extraction cartridge 11 are formed. It is engaged and held by an engagement member (not shown) in the cartridge holder 62. The engaging member is movable, and at the time of movement, the engagement with the protrusion 11d is released and the extraction cartridge 11 is dropped and discarded all at the same time.

  The cartridge holder 62 has pin holes 62d on both sides of the upper surface, and in use, a tip 49a of a presser pin 49 (see FIG. 1) described later is engaged and pushed downward. As shown in FIG. 3, when the cartridge holder 62 is raised, the lower end of the discharge portion 11 c of the extraction cartridge 11 held by the cartridge holder 62 is positioned above the waste liquid container 12 and the collection container 13 set in the container holder 63. However, as shown in FIG. 4, when the cartridge holder 62 is lowered, the discharge portion 11c of the extraction cartridge 11 is set to be inserted into the waste liquid container 12 or the collection container 13 by a predetermined amount.

  The container holder 63 is provided with waste liquid container holding holes 63a and recovery container holding holes 63b extending in the horizontal direction in two parallel rows, and a plurality of waste liquid containers 12 are arranged in the rear waste liquid container holding holes 63a. A plurality of collection containers 13 are respectively held in a row by 63b. The waste liquid container holding holes 63a and the collection container holding holes 63b are disposed at the same position as the holding holes 62c of the cartridge holder 62 at the same pitch, and the waste liquid containers 12 and the recovery containers 13 are respectively provided below the plurality of extraction cartridges 11 held. It is set to be located. The waste liquid container 12 and the recovery container 13 are preferably different in size, shape, etc. to prevent confusion.

  The container holder 63 is biased forward by a biasing member (not shown) built in the stand 61. In the container exchange movement (back and forth movement) of the container holder 63, the operating member 31 (see FIG. 2) installed on the mounting base 21 is engaged with the bottom of the container holder 63 through the opening formed in the bottom plate 61 b of the stand 61. It is performed by engaging with a hole (not shown). The container holder 63 is moved backward in accordance with the movement operation of the operation member 31 according to the drive of the container replacement motor 32 (DC motor), and the collection container 13 is operated below the cartridge holder 62. When not operating, the waste liquid container 12 is biased by a biasing member (not shown) so as to be positioned below the cartridge holder 62. The operation of the container replacement motor 32 is controlled according to the detection of the position sensors 33a and 33b.

  In addition, the waste liquid container holding hole 63a and the recovery container holding hole 63b are provided with a bottom, and even if liquid is accidentally dropped in a state where the waste liquid container 12 or the recovery container 13 is not set, contamination occurs due to outflow. It is supposed not to.

<Pressurized air supply mechanism>
The pressurized air supply mechanism 4 includes a plurality of (eight in the figure) air nozzles 41 arranged in a row on a pressure head 40 that moves up and down relative to the rack 6 of the mounting mechanism 3; As shown in the air system diagram, an air pump 43 that generates pressurized air, a relief valve 44 that opens the air passage to the atmosphere, and a supply of pressurized air by the air pump 43 that is installed for each air nozzle 41 is individually opened and closed. An open / close valve 45 and a pressure sensor 46 installed in each air nozzle 41 and detecting the internal pressure of the extraction cartridge 11 are provided.

  The pressure head 40 is held by a guide rod 24 installed in the vertical direction between the intermediate frame 22 and the upper frame 23 of the apparatus main body 2 so as to be vertically movable. Similarly, the ball nut 40a installed on the pressure head 40 is screwed to the ball screw 25 installed in the vertical direction, and the ball screw 25 is rotated through a timing belt and a pulley accompanying the driving of the elevating motor 47 (pulse motor). The pressure head 40 is moved up and down by the control accompanying the detection of the photosensors 48a to 48c. There are presser pins 49 on both sides of the pressure head 40. The presser pins 49 are urged downward by a spring 49b and can move up and down, and the tip 49a engages with a pin hole 62d on the upper surface of the cartridge holder 62. The position is restricted and can be pressed.

  The presser pin 49 presses the front position of the cartridge holder 62 so as not to interfere with the lateral movement of a cleaning liquid dispensing nozzle 51w and a collected liquid dispensing nozzle 51r, which will be described later, while the cartridge holder 62 is being pressed. It is arranged like this.

  Each of the air nozzles 41 is installed on the pressurizing head 40 so as to be vertically movable and urged downward. A sheet-like sealing material 42 having a communication hole 42a (see FIG. 2) corresponding to the air nozzle 41 is provided below the air nozzle 41. When the pressurizing head 40 moves downward, the upper end opening of the extraction cartridge 11 set in the cartridge holder 62 is pressed and sealed through the sealing material 42 at the tip of the air nozzle 41, and the communication hole 42a is sealed. Through this, pressurized air can be fed into the extraction cartridge 11.

  With the above-described configuration, the open / close valve 45 is opened to introduce pressurized air into the corresponding extraction cartridge 11, and the opening / closing is performed when the internal pressure detected by the pressure sensor 46 reaches the predetermined pressure range for the upper limit of pressurization. After the valve 45 is closed and sealed in a pressurized state, each liquid is discharged through the filter member 11b of the extraction cartridge 11 by pressurization, and the end of pressurization when the liquid discharge in each extraction cartridge 11 is completed is determined by each pressure. The sensor 46 individually detects the decrease in internal pressure when the liquid discharge is completed.

  At that time, the driving of the air pump 43 and the introduction of the pressurized air to the plurality of extraction cartridges 11 by the opening / closing operation of the opening / closing valve 45 according to the detection of the pressure sensor 46 are performed one by one in sequence, There is. When performing in order, one of the open / close valves 45 in the closed state is opened, pressurized air is introduced into the corresponding extraction cartridge 11, and the open / close valve is detected according to the detection of the pressure sensor 46. After closing 45, the next one open / close valve 45 is opened, pressurized air is introduced into the corresponding extraction cartridge 11, and the open / close valve 45 is closed according to the detection of the pressure sensor 46. This operation is repeated to introduce pressurized air into the plurality of extraction cartridges 11.

  When performing a plurality of operations simultaneously, the plurality of on-off valves 45 among all the on-off valves 45 that are closed are opened, pressurized air is simultaneously introduced into the respective extraction cartridges 11, and each on-off valve 45 is connected to each pressure sensor. In response to the detection of 46, the closed operation is performed to introduce pressurized air into the plurality of extraction cartridges 11. In the case of a plurality of simultaneous pressurizations, the discharge amount of the air pump 43 is controlled in accordance with the number of open / close valves 45 that are opened, and the pressure increase rate is made constant. The discharge amount control of the air pump 43 is performed by PWM control, for example. That is, the voltage applied to the air pump 43 is controlled so as to vary the time ratio according to the number of extraction cartridges 11 (on / off duty control), and the rotational speed of the air pump 43 is controlled to increase as the number of pressurization increases. The pressure increase rate of the pressurized air introduced into the extraction cartridge 11 is made uniform, and the sealed pressure when the pressure sensor 46 detects the predetermined pressure range for the upper limit of pressure and closes the opening / closing valve 45 is constant. To be.

  The relief valve 44 (FIG. 5) is normally in a closed state, and is opened to the atmosphere when the air in the passage from the air pump 43 to the opening / closing valve 45 is discharged. The opening / closing valve 45 is selectively opened, and an air circuit is configured to introduce pressurized air from the air pump 43 into the extraction cartridge 11 via the corresponding air nozzle 41. The pressure sensor 46 is installed in each air nozzle 41 and individually detects the internal pressure of the extraction cartridge 11, and the detected pressure falls within a predetermined pressure range for the upper limit of pressurization (for example, 50 to 200 kPa, preferably 80 to 120 kPa). The corresponding open / close valve 45 is closed to stop the supply of pressurized air, or the end of pressurization is determined by detecting the pressure drop associated with the completion of the liquid discharge from the extraction cartridge 11. Control such as shifting to a process is performed.

  The pressure sensor 46 detects the presence or absence of the extraction cartridge 11 set in the cartridge holder 62, detects the presence or absence of liquid, detects the lack of liquid amount, and detects filter clogging based on the pressure fluctuation in the extraction cartridge 11. ing. Details will be described later with reference to FIG.

  The release operation of the relief valve 44 shown in FIG. 5 is performed simultaneously with the opening operation of the corresponding opening / closing valve 45 according to the determination of the end of pressurization when the liquid from the extraction cartridge 11 is discharged, and remains in the extraction cartridge 11. The pressurized air to be discharged is removed to prevent liquid scattering due to the air ejection from the tip discharge portion 11c.

  As another form of the relief valve 44 for releasing the atmosphere, the relief valve 44 is opened to the atmosphere when all the open / close valves 45 are closed, and the excessively pressurized air from the continuously driven air pump 43 is released to continuously operate. During this time, the pressurized air whose pressure has increased is prevented from being suddenly supplied into the extraction cartridge 11 as the opening / closing valve 45 is opened. Further, the relief valve 44 may be opened to the atmosphere so as to obtain both functions.

  In the present embodiment, the open / close valve 45 is used, but the present invention is not limited to this, and the present invention can be applied by using an air pump and sealing it.

  Moreover, although the air pump 43 quoted by the said embodiment is an example of a diaphragm type pump, if it becomes pressurized air sources, such as a plunger type pump and a syringe pump, it is employable.

<Dispensing mechanism>
The dispensing mechanism 5 divides the cleaning liquid W contained in the cleaning liquid dispensing nozzle 51w and the recovered liquid dispensing nozzle 51r installed in the nozzle moving base 50 that can move horizontally on the rack 6 and the cleaning liquid W contained in the cleaning liquid bottle 56w. The cleaning liquid supply pump 52w for feeding to the injection nozzle 51w, the recovery liquid supply pump 52r for supplying the recovery liquid R accommodated in the recovery liquid bottle 56r to the recovery liquid dispensing nozzle 51r, and the mounting base 21 are mounted. A waste bottle 57 is provided.

  The nozzle moving table 50 is held by a guide rail 27 installed in the horizontal direction on the vertical wall 26 of the apparatus main body 2 and can move in the horizontal direction, and the movement is performed by a nozzle moving motor (pulse motor) (not shown). Drive control is performed so that the ink cartridge is sequentially stopped on the extraction cartridge 11 and stopped on the waste liquid bottle 57 in the return state. The cleaning liquid dispensing nozzle 51w and the recovered liquid dispensing nozzle 51r are bent at the tips downward, the cleaning liquid dispensing nozzle 51w is connected to the cleaning liquid supply pump 52w via the switching valve 55w, and the cleaning liquid supply pump 52w is switched to the switching valve 55w. The recovery liquid dispensing nozzle 51r is connected to the recovery liquid supply pump 52r via the switching valve 55r, and the recovery liquid supply pump 52r is connected to the recovery liquid bottle 56r via the switching valve 55r. Has been. The cleaning liquid bottle 56w and the recovery liquid bottle 56r are attached to the side portions of the apparatus main body 2, respectively. The cleaning liquid supply pump 52w and the recovery liquid supply pump 52r are constituted by syringe pumps, and piston members of the cleaning liquid W and the recovery liquid of a predetermined amount based on detection of the positions of the sensors 54w and 54r by pump motors 53w and 53r (pulse motors), respectively. The drive is controlled to dispense R.

  That is, when dispensing the cleaning liquid W or the recovery liquid R, the switching valve 55w or 55r is switched to the cleaning liquid bottle 56w or the recovery liquid bottle 56r, and the pump motor 53w or 53r is driven to drive the cleaning liquid supply pump 52w or the recovery liquid. The piston member of the supply pump 52r is moved backward, the cleaning liquid W or the recovery liquid R is sucked and accommodated in the cleaning liquid supply pump 52w or the recovery liquid supply pump 52r, and then the switching valve 55w or 55r is recovered in the cleaning liquid dispensing nozzle 51w or recovery. Switching to the liquid dispensing nozzle 51r side, driving the pump motor 53w or 53r to push the piston member of the cleaning liquid supply pump 52w or the recovered liquid supply pump 52r until the air in the passage is discharged to the waste liquid bottle 57 The cleaning liquid or the recovered liquid is used as the cleaning liquid dispensing nozzle 51w or the recovered liquid. Note After ejected from the nozzle 51r, it stops the driving of the cleaning liquid supply pump 52w or the recovering solution feeding pump 52r. After that, the cleaning liquid dispensing nozzle 51w or the recovered liquid dispensing nozzle 51r is moved onto the extraction cartridge 11, and then the driving amount of the cleaning liquid supply pump 52w or the recovered liquid supply pump 52r is controlled to control a predetermined amount of the cleaning liquid W or the recovered liquid. R is dispensed into the extraction cartridge 11.

  Each of the mechanisms 3 to 5 described above corresponds to an input operation of the operation panel 7 installed on the upper portion of the apparatus main body 2 and is driven and controlled based on a program built in a linked control unit (not shown). Is done.

  The extraction operation by the nucleic acid extraction apparatus 1 will be specifically described. First, the extraction cartridge 11 is set in the cartridge holder 62 of the rack 6 of the mounting mechanism 3, the waste liquid container 12 and the recovery container 13 are set in the container holder 63, and the rack 6 is mounted on the mounting base 21 of the apparatus main body 2. Prepare. Next, the dissolved sample solution S is sequentially injected into each extraction cartridge 11 with a pipette or the like. Note that the sample solution S may be injected first into the extraction cartridge 11 after being set in the rack 6 before being mounted on the apparatus 1 or before being set.

  Thereafter, when the apparatus is operated by operating the operation panel 7, the pressure head 40 is moved downward by driving of the lifting motor 47 of the pressure air supply mechanism 4, and the tip 49 a of the presser pin 49 is moved to the pin hole 62 d of the cartridge holder 62. The cartridge holder 62 is lowered to regulate the position, and the lower end discharge portion 11c of the extraction cartridge 11 is inserted into the waste liquid container 12 as shown in FIG. Make sure that it does not leak due to splashing etc. and cause contamination. Further, the pressurizing head 40 moves downward, and the lower end portion of each air nozzle 41 is pressed against the upper end opening of the extraction cartridge 11 through the sealing material 42 to be sealed. Since the presser pins 49 regulate the position of the cartridge holder 62, each air nozzle 41 can be accurately pressed against each extraction cartridge 11 to ensure a reliable sealing.

  Thereafter, pressurized air is supplied. First, the first opening / closing valve 45 is opened, and the air pump 43 is driven while the remaining opening / closing valve 45 is closed. When the pressurized air from the air pump 43 is supplied to the first extraction cartridge 11 through the first air nozzle 41 and rises to a predetermined pressure range for the upper pressure limit by detection of the pressure sensor 46, the first opening / closing valve 45 is provided. Is closed, the pressure to the extraction cartridge 11 is sealed in a pressurized state, the relief valve 44 is opened, and then the second opening / closing valve 45 is opened and the relief valve 44 is closed. Pressurized air is supplied to the second extraction cartridge 11 through the second air nozzle 41. This operation is repeated in order to apply pressure to all the extraction cartridges 11. When the supply of pressurized air to all the extraction cartridges 11 is completed and all the open / close valves 45 are closed, the driving of the air pump 43 is stopped and the relief valve 44 is opened to the atmosphere.

  In the extraction cartridge 11 into which the pressurized air is introduced, the sample liquid S is adsorbed and held by the nucleic acid through the filter member 11b by the action of pressure, and other liquid components are transferred to the waste liquid container 12 from the discharge part 11c at the lower end. Discharged. When all of the sample liquid S passes through the filter member 11b, the internal pressure suddenly decreases when the liquid discharge is completed, and when this pressurization is detected by the pressure sensor 46, the corresponding opening / closing valve 45 is opened to perform extraction. Pressurized air remaining in the cartridge 11 is released to the atmosphere via the relief valve 44 to prevent it from being ejected together with the liquid from the discharge portion 11 c of the extraction cartridge 11. When the end of pressurization is detected in all the extraction cartridges 11 by the pressure sensors 46, the pressurization head 40 is raised.

  The internal pressure accompanying the introduction of pressurized air into the extraction cartridge 11 fluctuates with the characteristic indicated by the curve A in FIG. 6 in the normal operation state, and the curve B is a differential waveform indicating the amount of change. First, the pressure rises linearly from time 0 when the open / close valve 45 is opened and pressurized air is introduced, and a predetermined pressure range for pressurization upper limit (for example, 50 to 200 kPa, preferably 80 to 120 kPa). The opening / closing valve 45 is closed at the point a that has reached the position, and the internal space of the extraction cartridge 11 is sealed in a pressurized state. The pressure is applied to the liquid so that it passes through the filter member 11b, and the pressure decreases as the liquid gradually decreases. At the point b, the entire amount of liquid passes through the filter member 11b and is discharged. When is completed, the air resistance at the filter member 11b is reduced and the pressure is rapidly reduced. Since the pressure fluctuation is noticeably generated in the differential waveform curve B, it is detected and the end of pressurization is determined.

  The determination of the end of pressurization is made when the amount of change in pressure drop per unit time is greater than or equal to a predetermined value, or because the detected pressure is less than or equal to a predetermined pressure range for determining the end of pressurization. It is preferable to reduce the time by using these together.

  In the case of pressurization of the sample liquid S, the pressure fluctuation varies depending on the viscosity and the like, and in the liquid having a low viscosity, the degree of pressure drop reaching the point b after the point a is increased, and the time until the point b is shortened. Become. In addition, when the viscosity of the liquid is high and the filter member 11b is slightly clogged, the degree of pressure drop reaching the point b after the point a is reduced, and the time until the point b is increased.

  Then, detection of a poor pressurization state from the fluctuation characteristics of the internal pressure detected by the pressure sensor 46, for example, detection of presence / absence of the set of the extraction cartridge 11, detection of presence / absence of liquid, detection of defective sealing, and detection of insufficient liquid amount Filter clogging judgment detection is performed.

  First, in order to detect a poor pressurization state, the pressure detected by the pressure sensor 46 is set to a low pressure of, for example, 10 kPa even after a predetermined time has elapsed since the open / close valve 45 is opened and the pressurized air is ejected from the air nozzle 41. This is performed when the pressure is within a predetermined pressure range for failure determination and the pressure does not rise further. In this case, the abnormal state is low in the air resistance and the extraction cartridge 11 is not set, or the sample liquid It can be determined that no air is injected or that the air nozzle 41 and the extraction cartridge 11 are in a poorly sealed state.

  When the sample liquid S is injected into the extraction cartridge 11 in a small amount rather than a specified amount, the initial pressure rises above the predetermined pressure range for determining the pressurization failure, but the opening / closing valve 45 is closed. It does not increase to the predetermined pressure range for the upper limit of pressurization, and it is determined that the liquid amount is insufficient when the discharge of the liquid is completed halfway and the pressure rapidly decreases.

  On the other hand, the detection pressure of filter clogging gradually decreases as the liquid is discharged, but this pressure drop is small, and even after a predetermined time has passed, the above-described determination of completion of pressurization at the completion of liquid discharge cannot be made. When the pressure does not fall below the predetermined pressure range for determining the end of pressure, it is determined that the filter is clogged. Further, the filter clogging may be detected when the time during which the pressure drop change amount does not become a predetermined value or more continues for a predetermined time or longer.

  The detection of defective pressurization when the pressure rise is insufficient at the time of pressurization, detection of pressurization completion upon completion of liquid discharge, and detection of filter clogging are performed in the same way in the cleaning process and the recovery process described later.

  Next, the process proceeds to a cleaning process, but the rise of the pressure head 40 after the supply of pressurized air stops at a position where the air nozzle 41 has moved away from the extraction cartridge 11 and moved to a height that allows the movement of the nozzle moving table 50 to be allowed. Then, the holding pin 49 presses the cartridge holder 62, and the state shown in FIG. 4 in which the lower end of the extraction cartridge 11 is inserted into the waste liquid container 12 is maintained. Then, the nozzle moving base 50 is moved to stop the cleaning liquid dispensing nozzle 51 w on the first extraction cartridge 11 to dispense a predetermined amount of the cleaning liquid W, and the nozzle moving base 50 is moved to the next extraction cartridge 11. The cleaning liquid W is dispensed sequentially. When dispensing of the cleaning liquid W to all the extraction cartridges 11 is completed, the pressure head 40 is moved downward, and the lower end portion of each air nozzle 41 is pressed against the upper end opening of the extraction cartridge 11 via the sealing material 42 and sealed. Then, supply pressurized air.

  In this cleaning process, a plurality of pressurized air is supplied at the same time, except for the extraction cartridge 11 at the location where the above-described pressurized failure state or the location where the filter is clogged has occurred. A plurality of open / close valves 45 corresponding to 11 are simultaneously opened to all the extraction cartridges in the operating state, and pressurized air by the air pump 43 variably driven by PWM control is introduced. Upon detection, the opening / closing valve 45 is closed. Further, similarly to the above, the open / close valve 45 may be sequentially opened to introduce pressurized air into each extraction cartridge 11.

  The cleaning liquid W on which the pressure is applied passes through the filter member 11b to clean and remove impurities other than nucleic acids, and the cleaning liquid W is discharged to the waste liquid container 12 from the discharge portion 11c at the lower end. When all the cleaning liquid W in all the extraction cartridges 11 is discharged through the filter member 11b, the pressure head 40 is raised to the initial position. The above operation is repeated when the cleaning process is performed a plurality of times.

  Next, the process proceeds to collection processing. After the pressurizing head 40 is lifted after the cleaning process, the presser pin 49 is raised and the cartridge holder 62 of the rack 6 is also moved upward, and the lower end discharge portion 11c of the extraction cartridge 11 is moved upward from the waste liquid container 12. Then, the operation member 31 of the mounting mechanism 3 is operated to move the container holder 63 backward, and the container replacement is performed so that the collection container 13 is positioned below the extraction cartridge 11.

  Subsequently, the pressure head 40 moves downward, and the tip of the presser pin 49 engages and presses against the pin hole 62 d of the cartridge holder 62. Then, the nozzle moving table 50 is moved to stop the collected liquid dispensing nozzle 51r on the first extraction cartridge 11 to dispense a predetermined amount of the collected liquid R, and the nozzle moving table 50 is moved to the next extraction cartridge 11. Then, the recovered liquid R is dispensed sequentially. When dispensing of the recovered liquid R to all the extraction cartridges 11 is completed, the pressurizing head 40 is further lowered in the same manner as described above, and the lower end portions of the air nozzles 41 are inserted into the upper end openings of the extraction cartridges 11 through the sealing material 42. After the pressure contact and sealing, a plurality of open / close valves 45 in a normal operating state are simultaneously opened to introduce pressurized air. Alternatively, the open / close valve 45 may be sequentially opened to introduce pressurized air into each extraction cartridge 11. The recovery liquid R on which the pressure is applied passes through the filter member 11b to release the nucleic acid adsorbed thereto, and the nucleic acid together with the recovery liquid R is discharged to the recovery container 13 from the discharge portion 11c at the lower end. When all the recovery liquid R in all the extraction cartridges 11 is discharged to the recovery container 13, the pressurizing head 40 is raised and the series of operations is completed.

  The rack 6 for which the extraction operation has been completed is lowered from the mounting table 21, the extraction cartridge 11 and the waste liquid container 12 are taken out from the cartridge holder 62 and the container holder 63 and discarded, while the collection container 13 is taken out from the container holder 63, The lid is covered as necessary, and the next nucleic acid analysis process or the like is performed.

  FIG. 7 is an air system diagram of another embodiment of the pressurized air supply mechanism 4. The pressurized air supply mechanism 4 of this embodiment includes one air pump 43, a mechanical opening / closing valve 145 that opens and closes the introduction of pressurized air to each extraction cartridge 11, and the discharge pressure of the air pump 43. Is provided with a pressure sensor 146. The mechanical opening / closing valve 145 has a characteristic of closing at the upper limit set pressure for the upper limit of pressurization and opening at the lower limit set pressure, and introduces pressurized air to the corresponding extraction cartridge 11 in the opened state, When the upper limit set pressure is reached, it is closed and sealed in a pressurized state to apply pressure to each liquid. Further, the mechanical opening / closing valve 145 is opened when the pressure is lowered when the liquid discharge from the extraction cartridge 11 is completed, and the next pressurized air can be introduced.

  In the pressurized air supply mechanism 4 provided with the mechanical opening / closing valve 145 of the present embodiment, the pressure sensor for individually detecting the internal pressure and the control based on the detection are not required, and the mechanism can be simplified.

  In the present embodiment, the cleaning process using the cleaning liquid W is performed, but this is not always necessary depending on the filtering ability of the filter member 11b.

  Moreover, although the nucleic acid extraction apparatus is described in the above embodiment, the present invention is not limited to this, and can be applied to a method of bringing various specific substances into contact with the filter member. It is not always necessary to recover using the recovery liquid, and it is possible to analyze the specific material in contact with the filter member, or to analyze the reaction color by adding the processing liquid.

The perspective view which shows the state which removed the cover of the nucleic acid extraction apparatus in one embodiment of this invention Schematic diagram of nucleic acid extraction device Perspective view of rack in mounting mechanism The perspective view which shows the use condition of a rack Air system diagram of pressurized air supply mechanism Diagram showing fluctuation of internal pressure of extraction cartridge Air system diagram showing another embodiment of the pressurized air supply mechanism Process diagram of extraction operation Perspective view of extraction cartridge

Explanation of symbols

1 Extraction device (nucleic acid extraction device)
2 Device body 3 Mounting mechanism 4 Pressurized air supply mechanism 5 Dispensing mechanism 6 Rack
11 Extraction cartridge
11b Filter material
12 Waste container
13 Collection container
40 Pressure head
41 Air nozzle
43 Air pump
44 Relief valve
45 Open / close valve
46 Pressure sensor
49 Presser pin
50 nozzle moving table
51w, 51r dispensing nozzle
52w, 52r supply pump
56w, 56r bottle
61 Stand
62 Cartridge holder
63 Container holder
145 Mechanical open / close valve S Sample liquid W Cleaning liquid R Recovery liquid

Claims (10)

In an extraction apparatus using an extraction cartridge provided with a filter member, injecting a sample liquid containing a specific substance into the extraction cartridge and adsorbing the specific substance in the sample liquid to the filter member,
A pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes an air pump and a pressure sensor that detects an internal pressure of the extraction cartridge, introduces pressurized air into the extraction cartridge, and the internal pressure falls within a predetermined pressure range. The extraction apparatus is characterized in that it is sealed when it reaches the position and a predetermined pressure is applied to the extraction cartridge.   The extraction apparatus according to claim 1, wherein there are a plurality of extraction cartridges, and the pressure sensor individually detects an internal pressure of each extraction cartridge. Using an extraction cartridge equipped with a filter member, a sample liquid containing nucleic acid is injected into the extraction cartridge and pressurized to adsorb the nucleic acid in the sample liquid to the filter member, and then the recovered liquid is dispensed into the extraction cartridge. In an extraction device that separates the nucleic acid adsorbed on the filter member by pressurization and collects it together with the recovery liquid,
The pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes an air pump, an open / close valve that opens and closes introduction of the pressurized air into the extraction cartridge, and a pressure sensor that detects an internal pressure of the extraction cartridge, Opening / closing valves are opened to introduce pressurized air into the corresponding extraction cartridge, and when the internal pressure reaches a predetermined pressure range, the opening / closing valve is closed and sealed in a pressurized state. An extraction device characterized by applying pressure.   Following opening of one of the open / close valves that are all closed, introducing pressurized air to the corresponding extraction cartridge, and closing the open / close valve in response to detection of the pressure sensor. Then, the operation of opening the next one open / close valve, introducing the pressurized air to the corresponding next extraction cartridge, and repeating the operation of closing the open / close valve in response to the detection of the pressure sensor, a plurality of the extraction 4. The extraction device according to claim 1, wherein pressurized air is introduced into the cartridge.   Opening a plurality of open / close valves among all the open / close valves that are in a closed state, simultaneously introducing pressurized air to the corresponding extraction cartridges, and closing the open / close valves in response to detection by the pressure sensors. The extraction apparatus according to claim 1, wherein pressurized air is introduced into the plurality of extraction cartridges.   6. The extraction device according to claim 5, wherein the discharge amount of the air pump is controlled according to the number of opening operations of the open / close valve, and the pressure increase rate is made constant.   After the pressurized air introduced into the extraction cartridge is sealed by closing the open / close valve, the pressure end point when the liquid discharge is completed through the filter member in the extraction cartridge is reduced by the pressure sensor when the liquid discharge is completed. The extraction apparatus according to any one of claims 1 to 3, wherein each of the extraction apparatuses is detected individually. Using an extraction cartridge provided with a filter member, a sample liquid containing nucleic acid is injected into the extraction cartridge and pressurized to adsorb the nucleic acid in the sample liquid to the filter member, and then the recovered liquid is dispensed into the extraction cartridge. An extraction device that performs an extraction operation of separating and collecting the nucleic acid adsorbed on the filter member by pressurization and collecting it together with a recovery liquid,
The pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes an air pump and a mechanical opening / closing valve that individually opens and closes the introduction of pressurized air into the extraction cartridge, and the mechanical opening / closing valve is pressurized. It has a characteristic of closing at the upper limit set pressure for the upper limit and opening at the lower limit set pressure. When the air reaches the upper limit set pressure by introducing pressurized air into the corresponding extraction cartridge in the open state Then, the extraction device is sealed in a pressurized state to cause pressure to act on the injected liquid, and is opened when the pressure drops when the liquid discharge from the extraction cartridge is completed. Using an extraction cartridge equipped with a filter member, a sample liquid containing nucleic acid is injected into the extraction cartridge and pressurized to adsorb the nucleic acid in the sample liquid to the filter member, and then the recovered liquid is dispensed into the extraction cartridge. In an extraction device that separates the nucleic acid adsorbed on the filter member by pressurization and collects it together with the recovery liquid,
A pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes one air pump, an open / close valve that opens and closes introduction of the pressurized air into the extraction cartridge, a pressure sensor that detects an internal pressure of the extraction cartridge, A relief valve for releasing the pressure of the air passage between the air pump and the opening / closing valve to release the atmosphere, introducing pressurized air into the extraction cartridge and sealing it, and detecting the pressure sensor from the extraction cartridge When the liquid discharge is completed, the opening / closing valve is opened and the relief valve is opened to the atmosphere, and the pressurized air remaining in the extraction cartridge is opened to the atmosphere via the opening / closing valve and the relief valve. Feature extraction device. Using an extraction cartridge equipped with a filter member, a sample liquid containing nucleic acid is injected into the extraction cartridge and pressurized to adsorb the nucleic acid in the sample liquid to the filter member, and then the recovered liquid is dispensed into the extraction cartridge. In an extraction device that separates the nucleic acid adsorbed on the filter member by pressurization and collects it together with the recovery liquid,
A pressurized air supply mechanism that introduces pressurized air into the extraction cartridge includes one air pump, an open / close valve that individually opens and closes the introduction of pressurized air into each extraction cartridge, and the internal pressure of each extraction cartridge. A pressure sensor for detecting, and a relief valve for releasing the pressure of the air passage between the air pump and the opening / closing valve to the atmosphere, and opening the one opening / closing valve to supply pressurized air to the corresponding extraction cartridge. After the supply, the open / close valve is closed, and when all the open / close valves are in the closed state, the relief valve is opened to the atmosphere, and the excessively pressurized air accompanying the continued driving of the air pump is passed through the relief valve. An extraction device that is open to the atmosphere.

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