JP2005089417A - Composition for prevention/treatment of diabetes - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition for the prevention/amelioration of diabetes having a suppressing effect on the increase in blood sugar level and an improving effect on glucose tolerance. <P>SOLUTION: This composition for the prevention/amelioration of diabetes contains a Matsutake mushroom having the suppressing effect on the increase in blood sugar level and the improving effect on the glucose tolerance as an active component. Usable species of the Matsutake mushroom are Tricholoma matsutake (S.Ito et Emai) Singer belonging to the genus Tricholoma, family Tricholoma and its related species; Tricholoma caligatum and Tricholoma Ponderosum. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a composition for preventing / treating diabetes, comprising matsutake as an active ingredient.

  Obesity and diabetes are continually increasing, mainly due to excessive caloric intake. There are two types of diabetes, insulin-dependent diabetes (type I diabetes) and non-insulin-dependent diabetes (type II diabetes), of which 90% are of the latter type. The cause of hyperglycemia in non-insulin-dependent diabetes mellitus is a lack of an early phase of insulin that should be secreted instantaneously and a decrease in endogenous basal insulin secretion induced by persistence of postprandial hyperglycemia in response to a rapid increase in blood glucose after meals. Can be mentioned. Correction of postprandial hyperglycemia may be difficult even with an oral hypoglycemic agent and insulin, and a method for preventing rapid absorption of carbohydrates in the digestive system is promising. As the absorption of carbohydrates in the digestive system, α-amylase in saliva is involved as the first step, and α-glucosidase in the small intestine is involved as the second step. By inhibiting these enzymes, blood glucose level after meals It has been clarified that the increase is suppressed and the subsequent increase in insulin level is also suppressed.

  Examples of such amylase activity inhibitors include wheat water extract (Patent Document 1), T-76 (Patent Document 2), N-61 (Patent Document 3), and X-2 (Patent Document 4) derived from Streptomyces microorganisms. ), I-1001 (patent document 5), AI-B (patent document 6), amylostatin A (patent document 7), tomatostatin derived from the genus Cladosporium (patent document 8), metabolites of the genus Glachyspoliera (patent document 8) Patent Document 9) and the like have already been disclosed.

  Moreover, as a glucosidase activity inhibitory substance, Ezoi Shige extract (patent document 10), Hongkage genus plant (patent document 11), acarbose, emiglitate, miglitol, voglibose (patent document 12), etc. have already been disclosed.

  However, the actual amylase activity inhibitors and glucosidase activity inhibitors that satisfy all of the stability, safety, and effectiveness have not yet been obtained.

The matsutake ( Tricholoma matsutake (S. Ito et Imai) Singer) used in the present invention is a basidiomycete belonging to the genus Kishimeji, and has a unique aroma and crispness. ing. Studies have been conducted on the ingredients and effectiveness of matsutake. Ergosterol contained in matsutake is used in combination with cyclodextrin to inhibit angiogenesis (see Patent Document 13), and β-glucan is used in combination with heat-treated cells of lactic acid-producing bacteria (Patent Document 14). Each of which is known to perform). It is also known that matsutake exhibits an antitumor effect (see Patent Documents 15 to 18). However, until now, it has not been known at all that matsutake has an effect of suppressing an increase in blood glucose level and an effect of improving glucose tolerance.

JP-A-5-139985 Japanese Patent Publication No.4-2604 JP-A-2-67299 Japanese Patent Publication No.54-11395 JP-A 61-74587 Japanese Patent Laid-Open No. 57-2684 JP 50-123891 A JP 57-11395 A Japanese Patent Laid-Open No. 8-12584 JP 2000-342224 A JP 2000-128793 A Special table 2002-529504 gazette JP 2002-308777 A JP 2003-40785 A JP 2000-159682 A Japanese Patent Laid-Open No. 5-70362 Japanese Patent Laid-Open No. 6-80699 JP-A-7-67576

  Accordingly, an object of the present invention is to provide a composition for preventing or improving diabetes having an effect of suppressing an increase in blood glucose level and an effect of improving glucose tolerance.

  As a result of various studies to solve the above-mentioned problems, the present inventors have found that matsutake exhibits an effect of suppressing an increase in blood glucose level and an effect of improving glucose tolerance, and has completed the present invention.

  The composition for preventing / ameliorating diabetes according to the present invention is derived from a mushroom, which is a natural product, has high safety, and has an action to suppress an increase in blood glucose level and an action to improve glucose tolerance. Ingesting foods can be expected to prevent diabetes and treat diabetes.

The matsutake used in the present invention includes Tricholoma matsutake (S. Ito et Imai) Singer, which belongs to the genus Xymetidae, which is distributed in Japan, the Korean Peninsula, Primorye, Sakhalin, and Kuril Islands. Hamamatsutake and American matsutake can also be used.

  Matsutake is a kind of mushroom belonging to the basidiomycete, and generally the fruiting body is used for food. In the present invention, it is preferable to use the fruiting body from the viewpoint of its effect.

  Matsutake may be used as it is or after it is dried. Such fruit body extracts can also be used, and extracts obtained by extraction using a solvent such as ethanol or water can also be used. From the viewpoint of the diabetes preventive / therapeutic effect of the present invention, it is most preferable to use a dried product, particularly a lyophilized product.

Examples 1-3 Preparation of freeze-dried powder of Matsutake fruiting body 3 kg of American Matsutake fruiting body, 3 kg of Japanese Matsutake fruiting body, and 3 kg of Satsuma matsutake fruiting body were processed in a freeze dryer and pulverized in a pulverizer. A freeze-dried powder of Matsutake fruiting bodies was prepared. The dried matsutake fruit body of Example 1 was used as Example 1, the dried matsutake fruit body of Japan as Example 2, and the dried dried matsutake fruit body as Example 3.

Examples 4-6 Supercritical carbon dioxide extract of Matsutake fruiting bodies Each of the dried Matsutake fruiting bodies of Examples 1-3 was put into a supercritical extraction apparatus, and a supercritical fluid of carbon dioxide under a pressure of 25 MPa at 40 ° C. Extracted using. The extract was recovered after 4 hours, and supercritical carbon dioxide extracts according to Examples 4 to 6 were obtained.

Examples 7 to 9 Hot water extract of Matsutake fruit bodies 20 kg of water was added to each 1 kg of dried Matsutake fruit bodies of Examples 1 to 3 and extracted at 90 ° C for 20 minutes. Thereafter, the extract was filtered to collect the filtrate, and hot water extracts according to Examples 7 to 9 were obtained.

Examples 10-12 Ethanol extract of Matsutake fruiting bodies 20 L of 50 vol% ethanol aqueous solution was added to each 1 kg of dried Matsutake fruiting bodies of Examples 1-3, and the mixture was extracted for 2 hours while stirring at room temperature. Thereafter, the extract was filtered to collect the filtrate, and ethanol extracts according to Examples 10 to 12 were obtained.

Effect on Type 1 Diabetes Using a streptozotocin (STZ) diabetic model rat, which is a type 1 diabetic model animal, the effect of matsutake mellitus on diabetes was examined as follows. About 4 weeks old male SD rats were given 65 mg / kg streptozotocin intraperitoneally to induce diabetes. One week later, rats that showed hyperglycemia, urine sugar, multiple meals, and multiple drinks were subjected to the following experiment. A group consisting of 10 STZ diabetic model rats prepared, and a basic diet group administered with a solid feed for rats (CRF-1 manufactured by Charles River Japan) as a basic diet, and the above-described implementation so that the basic diet is 5% by weight It divided into the Example 1-3 mixed food group which mixed the matsutake fruit body dried material of Examples 1-3. These were bred for 4 weeks in an environment where food and water could be freely ingested at room temperature of 23 ° C. and 12 hours light / dark cycle. Blood was collected from the rat tail vein after fasting for 20 hours every week from the start of the breeding, and the blood glucose level was measured. Furthermore, a glucose tolerance test was performed at 0, 2, and 4 weeks. That is, after fasting for 20 hours, 2 g / kg of glucose was orally administered using a sonde. Blood samples were collected from the tail vein before glucose administration (0 hours), 0 hours, 0.5 hours, 1 hour, 1.5 hours, and 2 hours after administration, and blood glucose levels were measured to calculate average values. Table 1 shows the changes in fasting blood glucose levels during the test period, and Tables 2 to 4 show the results of the glucose tolerance test.

  As can be seen from Table 1, it was shown that the increase in fasting blood glucose level tended to be suppressed in the example mixed diet group as compared with the basic diet group. Moreover, in the result of the glucose tolerance test, as shown in Table 2, there was no difference between the basic food group and the example mixed food group at the start of the test. In the week, the increase in blood glucose level immediately after glucose administration was suppressed, and the recovery of blood glucose level was accelerated. Therefore, compared with the basic food group, the glucose tolerance was improved in the Example mixed food group. From the above, it was shown that matsutake has an effect of preventing and improving type 1 diabetes.

Effect on type 2 diabetes Using the KKA ^Y diabetes model mouse, which is one of type 2 diabetes model animals, the effect of matsutake was examined in the same manner as in type 1 diabetes. About 4 weeks old KKA ^Y / Ta Jcl mice were preliminarily raised for 1 week, and 10 animals were grouped into a basic diet group and a working age mixed diet group, and the test was started. Mice were individually housed in cages and reared in an environment where food and water could be freely ingested in a light / dark cycle at room temperature of 23 ° C. for 12 hours. Every week from the start of breeding, blood was collected from the fundus of the mouse after fasting for 5 hours using a capillary blood collection tube, and the blood glucose level was measured. Furthermore, a glucose tolerance test was performed 36 days after the start of the breeding. That is, after fasting for 20 hours, 2 g / kg of glucose was orally administered using a sonde. Before glucose administration (0 hour), 0.5 hours, 1 hour, 1.5 hours, and 2 hours after administration, blood was collected from the fundus oculi, blood glucose level was measured, and an average value was calculated. Table 5 shows changes in blood glucose level during the test period, and Table 6 shows the results of the glucose tolerance test.

  As shown in Tables 5 and 6, it was confirmed that in the example mixed diet group, the increase in blood glucose level immediately after glucose administration was suppressed and the recovery of blood glucose level was quicker than that in the basic diet group. From the above, it was shown that matsutake has a preventive / ameliorating effect on type 2 diabetes.

Claims (1)

A composition for preventing or improving diabetes, comprising matsutake as an active ingredient.