JP2005073583A - New simplicillium microorganism having ability to control citrus disease injury and microbe-controlling method using the same - Google Patents
New simplicillium microorganism having ability to control citrus disease injury and microbe-controlling method using the same Download PDFInfo
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Abstract
Description
本発明は、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ（シンプリシリウム）属に属し、柑橘病害に対する防除能を有する新規微生物、並びにこれを用いた柑橘病害防除剤及び柑橘病害の生物防除方法に関する。 TECHNICAL FIELD The present invention relates to a novel microorganism belonging to the genus Simplicilium and having the ability to control citrus diseases, a citrus disease control agent using the same, and a biological control method for citrus diseases.
温暖多湿な我が国の柑橘類栽培では、年間を通じて多種多様な病害が発生し、薬剤（農薬）による病害防除は欠かせない技術となっている。しかし、近年、環境保全の面から農薬使用量の低減が望まれ、化学農薬（化学殺菌剤）に代わる生物防除技術の開発が要望されている。ここで、生物防除とは、一般に、病原体を抑制することができる別の微生物を用いて病原体を駆除することを意味する。 In citrus cultivation in Japan, which is warm and humid, a wide variety of diseases occur throughout the year, and disease control with chemicals (pesticides) has become an indispensable technology. However, in recent years, it has been desired to reduce the amount of agricultural chemicals used from the viewpoint of environmental protection, and development of biological control technology that replaces chemical agricultural chemicals (chemical fungicides) has been demanded. Here, biological control generally means extermination of a pathogen using another microorganism capable of suppressing the pathogen.
非病原性微生物を利用した植物の病害防除の研究は、イネや野菜分野においては盛んに行われているが、果樹の分野、特に柑橘類ではこれまで殆ど行われていない。 Research on disease control of plants using non-pathogenic microorganisms has been actively conducted in the field of rice and vegetables, but has hardly been conducted in the field of fruit trees, especially citrus fruits.
また、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ属に属する微生物を、植物病害の生物防除に使用した例もない。 In addition, there is no example of using a microorganism belonging to the genus Simplicilium for biological control of plant diseases.
本発明は、柑橘黒点病、柑橘灰色かび病、柑橘そうか病、柑橘かいよう病、柑橘褐色腐敗病、柑橘緑かび病、柑橘青かび病、柑橘軸腐病及び柑橘黒腐病などの柑橘病害を防除できる新規な微生物を提供することを目的とする。 The present invention relates to citrus diseases such as citrus black spot, citrus gray mold, citrus scab, citrus scab, citrus brown rot, citrus green mold, citrus blue mold, citrus stem rot and citrus black rot. It aims at providing the novel microorganisms which can be controlled.
また、本発明は、上記微生物を用いた、柑橘病害の生物防除剤及び柑橘病害の生物防除方法を提供することを目的とする。 Another object of the present invention is to provide a citrus disease biocontrol agent and a citrus disease biocontrol method using the microorganism.
上記目的を達成するため、本発明者らは鋭意研究を重ね、柑橘植物体から、柑橘病害に対する防除能を有する新規な糸状菌Ｓｉｍｐｌｉｃｉｌｌｉｕｍ ｌａｎｏｓｏｎｉｖｅｕｍ（シンプリシリウム・ラノソニベウム）（以下、ＳＥ−１菌株という）を見出し、この新規糸状菌の培養菌体を柑橘植物体に噴霧することによって、柑橘病害を防除できることを見出した。 In order to achieve the above-mentioned object, the present inventors have conducted intensive research, and from a citrus plant, a novel filamentous fungus Simplicilium lanosonicum (hereinafter referred to as SE-1 strain) having the ability to control citrus diseases. ) And found that citrus diseases can be controlled by spraying the cultured cells of this new filamentous fungus onto citrus plants.
なお、柑橘病害の微生物を用いた生物防除法は、これまで全く知られておらず、シンプリシリウム属菌に関して、柑橘病害に対する防除効果を認めたのは本発明者らが初めてである。 In addition, the biological control method using the microorganisms of a citrus disease is not known at all until now, and the present inventors are the first to recognize the control effect with respect to a citrus disease regarding the genus Simplicilium.
すなわち、本発明の第１の態様は、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ ｌａｎｏｓｏｎｉｖｅｕｍに属する新規微生物ＳＥ−１菌株（ＦＥＲＭ Ｐ−１９４２３）を提供する。 That is, the first aspect of the present invention provides a novel microorganism SE-1 strain (FERM P-19423) belonging to Simplicilium lanosonicum.
本発明の第２の態様は、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ属に属する微生物の培養物及び／又は微生物菌体及び／又は微生物の代謝産物及び／又は微生物の胞子を有効成分として含有することを特徴とする柑橘病害防除剤を提供する。 A second aspect of the present invention is a citrus disease control characterized by containing, as an active ingredient, a culture of microorganisms belonging to the genus Simplicilium and / or microbial cells and / or metabolites of microorganisms and / or spores of microorganisms. Provide the agent.
本発明の第３の態様は、上記第２の態様の柑橘病害防除剤を、柑橘植物体、柑橘果実又は柑橘果実貯蔵箱に施用することを特徴とする柑橘病害の生物防除方法を提供する。 A third aspect of the present invention provides a biological control method for citrus diseases characterized by applying the citrus disease control agent of the second aspect to citrus plants, citrus fruits or citrus fruit storage boxes.
本発明の新規微生物ＳＥ−１菌株を、柑橘病害の防除に利用することにより、化学殺菌剤の使用量を低減でき、環境に悪影響を及ぼすことなく、柑橘病害を有効に防除することができる。 By using the novel microorganism SE-1 strain of the present invention for the control of citrus diseases, the amount of chemical fungicide used can be reduced, and the citrus diseases can be effectively controlled without adversely affecting the environment.
以下、本発明を詳細に説明する。
本発明の第１の態様は、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ ｌａｎｏｓｏｎｉｖｅｕｍに属する新規微生物ＳＥ−１菌株に関する。
Hereinafter, the present invention will be described in detail.
The first aspect of the present invention relates to a novel microorganism SE-1 strain belonging to Simplicilium lanosonicum.
本発明の新規微生物ＳＥ−１菌株は、柑橘病害に対する防除能を有する糸状菌であり、以下のようにして単離されたものである。 The novel microorganism SE-1 strain of the present invention is a filamentous fungus having the ability to control citrus diseases and has been isolated as follows.
［ＳＥ−１菌株の単離方法］
分離源：柑橘植物体（柑橘園にあった２０年生の温州ミカンの枯れ枝）
分離培地：ポテトデキストロース寒天培地（以下、ＰＤＡという；ｐＨ５．６；日水製薬社製、Ｃｏｄｅ：０５７０９）
分離方法：３０ｍＬの滅菌水を入れた３００ｍＬの三角フラスコに１ｃｍの長さに切った上記枯れ枝５ｇを入れ、室温で１時間振とうした。得られた振とう液１ｍＬを、１０ｍＬの滅菌水中に入れ、順次希釈した。各希釈液から０．１ｍＬをとり、ＰＤＡ平面培地上に塗沫し、２５℃で５〜７日間培養し、出現した単一集落の先端の一部を、新たなＰＤＡ平面培地に移植した。移植７日後、単一集落を確認した後、集落の一部（５×５ｍｍ）を、再度新たなＰＤＡ平面培地に移植した。移植７日後、単一集落であることを確認した後、集落の一部をＰＤＡ斜面培地に保存した。その後の菌株の管理は２０℃の室内にて行い、６ヶ月ごとにＰＤＡ斜面培地に移植して保存した。
[Method for isolating SE-1 strain]
Isolation source: Citrus plants (dead branches of 20-year-old mandarin oranges in the citrus garden)
Separation medium: Potato dextrose agar medium (hereinafter referred to as PDA; pH 5.6; manufactured by Nissui Pharmaceutical Co., Ltd., Code: 05709)
Separation method: A 300 mL Erlenmeyer flask containing 30 mL of sterilized water was charged with 5 g of the dead branch cut to a length of 1 cm and shaken at room temperature for 1 hour. 1 mL of the obtained shaking solution was placed in 10 mL of sterilized water and diluted in sequence. 0.1 mL was taken from each diluted solution, smeared on a PDA flat medium, cultured at 25 ° C. for 5 to 7 days, and a part of the tip of a single colony that emerged was transplanted to a new PDA flat medium. Seven days after transplantation, after confirming a single colony, a part of the colony (5 × 5 mm) was transplanted again into a new PDA flat medium. Seven days after transplantation, after confirming that it was a single colony, a part of the colony was stored in a PDA slant medium. Subsequent strain management was performed in a room at 20 ° C., and transplanted and stored in a PDA slant medium every 6 months.
上記のようにして単離された本発明の新規微生物ＳＥ−１菌株は、下記の微生物学的性質及び遺伝子解析により、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ ｌａｎｏｓｏｎｉｖｅｕｍであると同定した。本願出願人は、この微生物に「ＳＥ−１」の識別番号を付し、この微生物を産業技術総合研究所 特許生物寄託センターに寄託した。（微生物寄託番号 ＦＥＲＭ Ｐ−１９４２３） The novel microorganism SE-1 strain of the present invention isolated as described above was identified as Simplicilium lanosonicum by the following microbiological properties and gene analysis. The applicant of the present application assigned an identification number of “SE-1” to this microorganism, and deposited this microorganism at the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology. (Microorganism deposit number FERM P-19423)
［ＳＥ−１菌株の微生物学的性質］
菌そう：表面はビロード状で白色から茶色、黒緑色。裏面は白色から茶色、黒緑色。臭気はない。
[Microbiological properties of SE-1 strain]
Fungus-like: The surface is velvety, white to brown, blackish green. The back side is white to brown, blackish green. There is no odor.
菌糸：隔壁を有し、無色。 Mycelium: colorless and colorless.
分生子柄：透明で直立し、菌糸から直接立ち上がる。単一若しくは希に分岐、基部から先端にいくほど細くなり、先端に球状の分生子をつける。 Conidial pattern: Transparent and upright, standing up directly from the mycelium. A single or rare branch, thins from the base to the tip, and has a spherical conidia at the tip.
分生子形成細胞（フィアライド）：１本（希に２本）。 Conidia-forming cells (Fialide): 1 (rarely 2).
分生子：フィアライドの先端に着生し、透明、球形、亜球形、１細胞、大きさは２〜３×４〜５μｍ。生育温度域は５〜３５℃で、至適温度域は２５〜２７℃。 Conidia: It grows on the tip of the phialide, is transparent, spherical, subspherical, 1 cell, and has a size of 2 to 3 × 4 to 5 μm. The growth temperature range is 5 to 35 ° C, and the optimum temperature range is 25 to 27 ° C.
［遺伝子解析］
ＳＥ−１菌株について、２８ＳｒＲＮＡ遺伝子Ｄ２領域の３２１塩基配列を決定した。（３２１塩基配列については、本願明細書末尾の配列表を参照されたい。）
[Gene analysis]
For the SE-1 strain, the 321 nucleotide sequence of the 28S rRNA gene D2 region was determined. (Refer to the sequence table at the end of the present specification for the 321 nucleotide sequence.)
本発明のＳＥ−１菌株を培養するために用いられる培地の栄養源としては、通常の微生物の生育に必要であって、本菌が資化可能な栄養源であれば、如何なる炭素源、窒素源及び無機塩類等でもよい。 As a nutrient source of the medium used for culturing the SE-1 strain of the present invention, any carbon source, nitrogen can be used as long as it is necessary for normal microorganism growth and can be assimilated by the bacterium. Sources and inorganic salts may be used.
炭素源としては、例えば、ブドウ糖やショ糖等が利用できる。窒素源としては、硝酸塩やアンモニウム塩等が利用できる。無機塩類としては、リン酸塩やナトリウム等を用いることができる。 As the carbon source, for example, glucose or sucrose can be used. As the nitrogen source, nitrates, ammonium salts and the like can be used. As inorganic salts, phosphates, sodium and the like can be used.
本発明のＳＥ−１菌株は、柑橘病害に対する防除能、すなわち、柑橘病害の原因菌に対する拮抗作用を有する。ここで、柑橘病害とは、柑橘植物に悪影響を与える菌に起因する種々の病害をいい、具体的には、例えば、柑橘黒点病、柑橘灰色かび病、柑橘そうか病、柑橘かいよう病、柑橘褐色腐敗病、柑橘緑かび病、柑橘青かび病、柑橘軸腐病、柑橘黒腐病等が挙げられる。 The SE-1 strain of the present invention has an ability to control citrus diseases, that is, an antagonistic action against causative bacteria of citrus diseases. Here, citrus disease refers to various diseases caused by bacteria that adversely affect citrus plants. Specifically, for example, citrus black spot disease, citrus gray mold disease, citrus scab, citrus scab, citrus Examples include brown rot, citrus green mold, citrus blue mold, citrus stem rot, and citrus black rot.
次に、本発明の第２の態様である柑橘病害防除剤の製造方法について説明する。 Next, the manufacturing method of the citrus disease control agent which is the 2nd aspect of this invention is demonstrated.
本発明の柑橘病害防除剤は、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ属に属する微生物の培養物及び／又は微生物菌体及び／又は微生物の代謝産物及び／又は微生物の胞子を有効成分として含有する。すなわち、本発明の柑橘病害防除剤は、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ属に属する微生物の培養物、微生物菌体、微生物の代謝産物及び／又は微生物の胞子のいずれか１種以上を有効成分とする。好ましくは、本発明の柑橘病害防除剤は、Ｓｉｍｐｌｉｃｉｌｌｉｕｍ属に属する微生物の培養物、微生物菌体、及び／又は微生物の胞子のいずれか１種以上を有効成分とする。 The citrus disease control agent of the present invention contains a culture of microorganisms belonging to the genus Simplicilium and / or microbial cells and / or metabolites of microorganisms and / or spores of microorganisms as active ingredients. That is, the citrus disease control agent of the present invention contains, as an active ingredient, any one or more of a culture of microorganisms belonging to the genus Simplicilium, microbial cells, microbial metabolites, and / or spore of microorganisms. Preferably, the citrus disease control agent of the present invention contains as an active ingredient any one or more of a culture of microorganisms belonging to the genus Simplicilium, microbial cells, and / or spores of microorganisms.
Ｓｉｍｐｌｉｃｉｌｌｉｕｍ属に属する微生物としては、上記本発明のＳｉｍｐｌｉｃｉｌｌｉｕｍ ｌａｎｏｓｏｎｉｖｅｕｍ ＳＥ−１菌株が好ましい。 As the microorganism belonging to the genus Simplicilium, the above-mentioned Simplicilium lanosonicum SE-1 strain is preferable.
まず、上記本発明のＳＥ−１菌株を適当な培地で培養する。ここで使用する培地は、ＳＥ−１菌株が増殖するものであれば特に制限されず、通常使用されているポテトデキストロース寒天培地を使用すればよい。培養してＳＥ−１菌株を増殖させた後、遠心分離により集菌を行い（微生物菌体）、その使用目的や散布方法に応じて製剤化することができる。また、培養終了後、菌体を分離せずにそのまま（微生物の培養物）使用することもできる。さらには、微生物の培養液中に存在する、微生物の代謝産物又は微生物の胞子のみを分離して製剤化することもできる。 First, the SE-1 strain of the present invention is cultured in an appropriate medium. The medium used here is not particularly limited as long as the SE-1 strain grows, and a commonly used potato dextrose agar medium may be used. After culturing and proliferating the SE-1 strain, the cells are collected by centrifugation (microorganism cells) and can be formulated according to the purpose of use and the application method. Further, after completion of the culture, the cells can be used as they are (microorganism culture) without separating them. Furthermore, it is also possible to separate and formulate only the microbial metabolite or microbial spore present in the microbial culture solution.
次に、本発明の第３の態様である、柑橘病害の生物防除方法について説明する。 Next, the biological control method of a citrus disease which is the 3rd aspect of this invention is demonstrated.
本発明の柑橘病害の生物防除方法は、上記本発明の柑橘病害防除剤を、一般に用いられている施用方法により施用することからなる。 The biological control method of the citrus disease of this invention consists of applying the citrus disease control agent of the said this invention with the application method generally used.
柑橘病害防除剤の施用の対象は、通常は柑橘植物体であるが、柑橘果実のみ又は柑橘果実貯蔵箱に施用することもできる。 The target of application of the citrus disease control agent is usually a citrus plant, but it can also be applied only to citrus fruits or to a citrus fruit storage box.
施用方法としては、例えば、噴霧、浸漬、塗布、粉衣、注入処理などが挙げられ、特に噴霧処理が好ましい。これらの施用方法は、当分野で通常用いられる手法によって実施できる。 Examples of the application method include spraying, dipping, coating, powder coating, injection treatment, and the like, and spraying is particularly preferable. These application methods can be carried out by techniques commonly used in the art.
噴霧処理を行う場合の、柑橘病害防除剤の噴霧量は、柑橘植物１樹に対して、通常５〜１５Ｌ、好ましくは１０〜１５Ｌの範囲である。 The amount of spray of the citrus disease control agent in the case of performing a spray process is 5-15L normally with respect to 1 citrus plant, Preferably it is the range of 10-15L.
本発明の柑橘病害の生物防除方法における、施用頻度、施用時期等は、施用対象となる柑橘植物の生育周期に応じて適宜選択すべきであるが、例えば、ミカンであれば、６月及び７月の２回とするのが好ましい。 In the biological control method for citrus diseases of the present invention, the application frequency, the application time, and the like should be appropriately selected according to the growth cycle of the citrus plant to be applied. Preferably twice a month.
また、本発明の柑橘病害防除剤と化学殺菌剤（化学農薬）とを組み合わせて施用する（体系防除）こともできる。例えば、ミカンであれば、６月と８月には化学殺菌剤を散布し、７月に本発明の柑橘病害防除剤を散布するなどである。ここで、用いられる化学殺菌剤としては、柑橘病害に対して従来から用いられてきた薬剤を用いることができ、例えば、エムダイファー水和剤（クミアイ化学工業株式会社製）、ジマンダイセン水和剤（ダウ・ケミカル株式会社製）、ストロビードライフロアブル（クミアイ化学工業株式会社製）などが挙げられる。 Moreover, the citrus disease control agent of the present invention and a chemical fungicide (chemical pesticide) can be applied in combination (system control). For example, in the case of a mandarin orange, a chemical disinfectant is sprayed in June and August, and a citrus disease control agent of the present invention is sprayed in July. Here, as the chemical disinfectant used, a drug conventionally used for citrus diseases can be used. For example, emdaifer wettable powder (manufactured by Kumiai Chemical Industry Co., Ltd.), dimandaisen wettable powder (Dow)・ Made by Chemical Co., Ltd., Strobe Dry Dry Bull (made by Kumiai Chemical Industry Co., Ltd.), etc.
ミカン栽培において、化学殺菌剤は、通常６、７及び８月に薬剤散布を行うが、本発明の柑橘病害防除剤と化学殺菌剤とを組み合わせることにより、本発明の柑橘病害防除剤単独よりも効率的に柑橘病害を防除することができると共に、化学殺菌剤の散布回数を３回から２回に減らすことができ、化学殺菌剤の使用量を低減することができる。 In mandarin orange cultivation, chemical fungicides are usually sprayed in June, July and August, but by combining the citrus disease control agent of the present invention with the chemical fungicide, the citrus disease control agent of the present invention alone. While being able to control a citrus disease efficiently, the frequency | count of spraying of a chemical germicide can be reduced from 3 times to 2 times, and the usage-amount of a chemical germicide can be reduced.
以下、試験例を挙げて、本発明をさらに具体的に説明する。 Hereinafter, the present invention will be described more specifically with reference to test examples.
（試験例１）ＳＥ−１菌の柑橘黒点病に対する拮抗作用
方法：
柑橘黒点病菌のα胞子懸濁液（１０4／ｍＬ）とＳＥ−１菌の胞子懸濁液（１０4／ｍＬ）を等量混合したものを、試験管の底に０．３ｍＬ注入した。この試験管に高圧滅菌した５ｃｍ長の柑橘植物の枯れ枝を、１試験管あたり５本挿入し、１ヶ月後、枯れ枝上に形成された柑橘黒点病菌の胞子角を計数した。１区あたり５試験管（２５本の枯れ枝）を用いた。対照として、柑橘黒点病菌のみの区を設けた。
(Test Example 1) Antagonistic method against citrus black spot disease of SE-1 bacteria:
0.3 mL of a mixture of equal amounts of spore suspension (10 4 / mL) of citrus black spot fungus and spore suspension (10 4 / mL) of SE-1 was injected into the bottom of the test tube. Five dead citrus plants 5 cm long sterilized in this test tube were inserted per test tube, and one month later, the spore angle of the citrus black spot fungus formed on the dead branch was counted. Five test tubes (25 dead branches) were used per section. As a control, a section containing only citrus black spot fungus was provided.
結果：
結果を下記表１に示す。
result:
The results are shown in Table 1 below.
表１の結果から、ＳＥ−１菌を共存させることにより、柑橘黒点病菌の増殖が抑制できることがわかる。 From the results of Table 1, it can be seen that the growth of citrus black spot disease bacteria can be suppressed by coexisting SE-1 bacteria.
（試験例２）ミカン園における柑橘黒点病に対するＳＥ−１菌株の防除効果試験
方法：
（１）試験ほ場
静岡県清水市駒越西 柑橘試験場ほ場 ３ａ
（品種：青島温州８年生 １区１樹、３連制）
(Test Example 2) Test method for controlling effect of SE-1 strain against citrus black spot in mandarin orchard:
(1) Test field, Komizukoshi West, Shimizu City, Shizuoka Prefecture, Citrus Experiment Station Field 3a
(Variety: Qingdao Wenzhou 8th Grade, 1 tree, 1 tree, 3 continuous system)
（２）柑橘病害防除剤及び防除方法：
ＳＥ−１菌をポテトデキストロース液体培地で７日間振とう培養（２５℃）後、培養液原液を水道水で希釈し、１０6／ｍＬの菌液を作製し、噴霧器を使って散布した。
(2) Citrus disease control agent and control method:
After SE-1 bacteria were cultured in a potato dextrose liquid medium for 7 days with shaking (25 ° C.), the stock solution of the culture solution was diluted with tap water to prepare a 10 6 / mL bacterial solution, which was sprayed using a sprayer.
（３）試験区
ＳＥ−１菌散布区（１０6／ｍＬ、３Ｌ／樹；６月、７月の２回散布）
ジマンダイセン水和剤散布区（６００倍希釈、３Ｌ／樹、６月、７月、８月の３回散布）
無処理区（６月〜８月の期間殺菌剤無散布）
(3) Test area SE-1 bacteria spraying area (10 6 / mL, 3 L / tree; spraying twice in June and July)
Zymandene wettable powder spray (diluted 600 times, 3 L / tree, sprayed three times in June, July and August)
No treatment zone (no spraying of bactericide during the period from June to August)
（４）薬剤散布日
平成１２年６月２２日、７月２１日、８月２３日
(4) Drug application date June 22, July 21, 2000, August 23, 2000
（５）調査
柑橘黒点病の調査は平成１２年１１月９日に行い、下記計算式を用いて防除効果を求めた。
(5) Investigation The investigation of citrus black spot disease was conducted on November 9, 2000, and the control effect was obtained using the following formula.
発病果率（％）＝（発病果数÷調査果数）×１００ Disease incidence rate (%) = (number of diseased fruits ÷ number of surveyed fruits) × 100
発病度＝（(発病指数×該当発病果数)÷(７×調査果数)）×１００
発病指数 ０：病斑がないもの
１：病斑が散見されるもの
３：病斑が果面の１／４に分布するもの
５：病斑が果面の１／４〜１／２に分布するもの
７：病斑が果面の１／２以上に分布するもの
Disease severity = ((morbidity index × number of corresponding disease fruits) ÷ (7 × number of study fruits)) × 100
Disease index 0: No lesion
1: Some lesions appear
3: The lesion is distributed over 1/4 of the fruit surface
5: The lesion is distributed in 1/4 to 1/2 of the fruit surface
7: The lesion is distributed over 1/2 of the fruit surface
防除価＝１００−（(処理区の発病度÷無処理区の発病度)×１００） Control value = 100 − ((Severity of treated area ÷ Severity of untreated area) × 100)
結果：
結果を下記表２に示す。
result:
The results are shown in Table 2 below.
表２の結果から、ＳＥ−１菌は、化学殺菌剤であるジマンダイセン水和剤には及ばないものの、ジマンダイセン水和剤の６９％（（３３．６÷４８．４）×１００）の防除効果を示すことが明らかとなった。 From the results of Table 2, SE-1 bacteria do not reach the chemical fungicide dimandaisen wettable powder, but the control effect of 69% ((33.6 ÷ 48.4) × 100) of dimandaicene wettable powder. It became clear to show.
（試験例３）ミカン園における柑橘黒点病に対するＳＥ−１菌株からなる生物防除剤と化学殺菌剤との組み合わせによる防除効果試験
方法：
（１）試験ほ場
静岡県引佐郡三ヶ日町 柑橘試験場西遠分場ほ場 ２０ａ
（品種：宮川早生３７年生 １区１樹、３連制）
(Test Example 3) Control effect test method by combination of biocontrol agent composed of SE-1 strain and chemical fungicide against citrus sunspot in citrus orchard:
(1) Test field Shikaoka Prefecture Hisa-gun Mikkabi-cho Citrus Experiment Station Nishi Tobunjo Field 20a
(Variety: 37th grade Miyagawa, 1st ward, 1st tree, 3 consecutive systems)
（２）柑橘病害防除剤及び防除方法：
ＳＥ−１菌をポテトデキストロース液体培地で７日間振とう培養（２５℃）後、培養液原液を水道水で希釈し、１０6／ｍＬの菌液を作製し、噴霧器を使って散布した。
(2) Citrus disease control agent and control method:
After SE-1 bacteria were cultured in a potato dextrose liquid medium for 7 days with shaking (25 ° C.), the stock solution of the culture solution was diluted with tap water to prepare a 10 6 / mL bacterial solution, which was sprayed using a sprayer.
（３）試験区
体系防除ＳＥ−１菌散布区
６月：エムダイファー水和剤（６００倍希釈、３Ｌ／樹）
７月：ＳＥ−１菌（１０6／ｍＬ、３Ｌ／樹）
８月：ジマンダイセン水和剤（８００倍希釈、３Ｌ／樹）
慣行防除区
６月：エムダイファー水和剤（６００倍希釈、３Ｌ／樹）
７月：ジマンダイセン水和剤（８００倍希釈、３Ｌ／樹）
８月：ジマンダイセン水和剤（８００倍希釈、３Ｌ／樹）
無処理区（６月〜８月の期間殺菌剤無散布）
(3) Test Zone System Control SE-1 Bacteria Spray Zone June: Emdaifer wettable powder (600 times dilution, 3L / tree)
July: SE-1 bacteria (10 6 / mL, 3 L / tree)
August: Dimandaisen wettable powder (diluted 800 times, 3L / tree)
Practice control zone June: Emdaifer wettable powder (600 times dilution, 3L / tree)
July: Zymandene wettable powder (diluted 800 times, 3L / tree)
August: Dimandaisen wettable powder (diluted 800 times, 3L / tree)
No treatment zone (no spraying of bactericide during the period from June to August)
（４）薬剤散布日
平成１４年６月３日、７月１１日、８月１９日
(4) Drug application date June 3, 2002, July 11, August 19
（５）調査
柑橘黒点病の調査は平成１４年１０月１３日に行い、上記試験例２に記載の計算式を用いて防除効果を求めた。
(5) Investigation The investigation of citrus black spot disease was conducted on October 13, 2002, and the control effect was determined using the calculation formula described in Test Example 2 above.
結果：
結果を下記表３に示す。
result:
The results are shown in Table 3 below.
表３の結果から、ＳＥ−１菌からなる柑橘病害防除剤と化学殺菌剤との組み合わせからなる体系防除は、従来の化学殺菌剤のみを用いる慣行防除にほぼ匹敵する防除効果を示すことが明らかになった。 From the results in Table 3, it is clear that system control consisting of a combination of a citrus disease control agent consisting of SE-1 bacteria and a chemical fungicide exhibits a control effect almost comparable to conventional control using only conventional chemical fungicides. Became.
本発明によれば、環境に悪影響を及ぼすことなく、柑橘病害を有効に防除することができ、良好な柑橘果実を生産することが可能となる。 According to the present invention, it is possible to effectively control citrus diseases without adversely affecting the environment, and it is possible to produce good citrus fruits.
さらに、本発明の柑橘病害防除剤と化学殺菌剤とを組み合わせた体系防除により、化学殺菌剤のみによる場合とほぼ同等の防除効果が得られ、化学殺菌剤の使用量を低減することができる。 Furthermore, the system control combining the citrus disease control agent of the present invention and the chemical bactericidal agent can provide a control effect substantially equivalent to that of the chemical bactericidal agent alone, and the amount of the chemical bactericide used can be reduced.
Claims (7)
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| KR101013455B1 (en) | 2010-11-12 | 2011-02-14 | 주식회사 그린바이오텍 | Method and product of freeze drying of simplicillium lamellicola bcp |
| KR101013456B1 (en) * | 2010-11-24 | 2011-02-14 | 주식회사 그린바이오텍 | Composition for the culturing of simplicillium lamellicola bcp and culturing method using the composition |
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| CN113080248A (en) * | 2021-05-18 | 2021-07-09 | 华中农业大学 | Method for rapidly testing damage and simultaneously inhibiting bacteria after citrus picking |
| CN113080248B (en) * | 2021-05-18 | 2022-09-13 | 华中农业大学 | Method for rapidly testing damage and simultaneously inhibiting bacteria after citrus picking |
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