JP2005068129A - Edible composition having promoting activity against production and release of calcitonin gene-associated peptide - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain an edible composition having effects of preventing and treating gastric mucosa failure, lifestyle-related diseases, obesity, malignant tumor and osteoporosis, reducing organ transplant rejection reactions, preventing and reducing organ failure accompnied with living body invasion reactions against invading factors such as infections and further having a hair restoration effect, also gentle to a human body and having no adverse effect. <P>SOLUTION: This edible composition having a promoting activity against production and release of a calcitonin-associated peptide contains isoflavone and capsaicin as essential components. The edible composition is used for preventing and treating the gastric mucosa failure, lifestyle-related diseases, obesity, malignant tumor and osteoporosis, reducing the organ transplant rejection reactions, preventing and reducing the organ failure accompanied with the living body invasion reactions against the invading factors such as the infections and further restoring the hair, and the like. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an edible composition having a calcitonin gene-related peptide production and release promoting action. Specifically, the present invention relates to a bioinvasive response to invasive factors such as gastric mucosal injury, lifestyle-related diseases, obesity, malignant tumors, etc., prevention and treatment, osteoporosis prevention and treatment, organ transplant rejection reduction, infection, etc. The present invention relates to an edible composition for prevention and reduction of organ damage associated with hair and for hair growth.

  In modern society, it is impossible to live avoiding social, psychological and physical stress that more or less disturbs the internal environment of the living body. The importance of the relationship between stress and illness has been pointed out by social and psychological stressors in conditions that are deeply involved in their development, such as peptic ulcer, irritable bowel syndrome, bronchial asthma, hypertension, ischemic heart disease and diabetes However, in addition to these, it is also known that immunity decreases due to social and psychological stress, and the risk of infections and cancers increases. In addition, the number of men and women suffering from thinning hair and hair loss is increasing, but it has been pointed out that this is also influenced by stress in addition to factors such as genetic predisposition and aging.

  On the other hand, biological reactions to physicochemical stressors to the body, such as infection and trauma, are very similar to those to social psychological stressors, and inflammatory cytokines such as TNF-α are expressed in the expression of these biological reactions. It has become clear that is important.

  TNF-α is basically a substance that plays the most important role in living body defense in the stress reaction, activates monocytes, neutrophils, vascular endothelial cells and the like, and is greatly involved in the expansion of the inflammatory response. However, excessive production of TNF-α due to strong and continuous stress causes circulatory disturbances such as thrombus formation and apoptosis of parenchymal cells, thereby causing organ dysfunction and morphological changes, etc. It falls into liquid quality and various disease symptoms appear. From this point of view, if many etiologies are considered stressors, the response to them can be considered to be the pathology of the disease and clinical symptoms.

  For example, the development of peptic ulcer is caused by inflammation of the gastric mucosa caused by TNF-α. Further, it is known that increased production of TNF-α increases the risk of developing lifestyle-related diseases such as diabetes, hypertension, hyperlipidemia and arteriosclerosis, cancer, and osteoporosis.

A calcitonin gene-related peptide, which is a kind of neuropeptide, plays an important role in suppressing the production of TNF-α. The calcitonin gene-related peptide is a 37 amino acid peptide that is very similar in structure to the hormone calcitonin, which is on the same gene as the calcitonin gene and is generated by alternative splicing. . Calcitonin gene-related peptide suppresses TNF-α production and apoptosis of cancer cells by suppressing vasodilation, osteoblast proliferation, osteoclast activation inhibition, appetite inhibition, and NFκB activation. Promote. In addition, the production of prostaglandins such as NO and PGI ₂ is enhanced by the action of calcitonin gene-related peptides on vascular endothelial cells, but these substances suppress TNF-α production in addition to blood flow increasing action. Demonstrate the effect. Furthermore, calcitonin gene-related peptides are known to have an important effect on the circulatory system, including systemic vasodilatory action, positive chronotropic and inotropic action, NaCl extracorporeal action, coronary vasodilatory action, And has an effect of increasing renal blood flow.

Currently, various drugs are used for the treatment of the above-mentioned diseases that are thought to be caused by excessive production of TNF-α. However, many of them are symptomatic drugs and do not relate to the main cause of the pathological condition, but rather side effects are often problematic. For example, in the treatment of peptic ulcer, drugs that strongly suppress gastric acid secretion, such as H ₂ blocker, are often used. Although improvement of subjective symptoms such as gastric acid and superficial self-digestion of gastric mucosa by gastric acid are reduced, inflammation of gastric mucosa is not alleviated, but rather aggravates and forms the origin of gastric mucosal atrophy and eventually gastric cancer It will be.

Steroids also inhibit TNF-α production and have anti-inflammatory effects, but at the same time disrupt organ protection mechanisms. In addition, indomethacin, a non-steroidal anti-inflammatory agent, alleviates the symptoms of fever, pain and swelling by suppressing the production of large amounts of PGE ₂ induced by TNF-α, but at the same time protects organs. Inhibits COX-1, which is required for the production of prostaglandins that are important for

By the way, as described above, the calcitonin gene-related peptide suppresses excessive production of TNF-α causing the above-mentioned harmful effects and induces an insulin-like growth factor. Insulin-like growth factors are thought to promote hair follicle growth and play an important role in the transition from the resting phase to the growth phase in the hair cycle and the extension of the growth phase.
Kenji Okajima, “Bioinvasion and disease-from severe sepsis to lifestyle-related diseases”, Hyundai Medical Company, 2003 Harada N, Okajima K, Uchiba M, Katsuragi T, Contribution of capsaicin-sensitive sensory neurons to stress-induced increase in gastric tissue levels of prostaglandins in rats, Am J Physiol Gastrointest Liver Physiol, 2003 Jul 31 [Epub ahead of print] Vignery A, McCarthy TL, The neuropeptide calcitonin gene-related peptide stimulates insulin-like growth factor 1 production by primary fetal rat osteoblasts, Bone, 1996, Vol 18, No 4, 331-335 Philpott MP, Sanders DA, Kealey T, Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-1 at physiologic concentrations is an important regulator of hair follicle growth in vitro, J Invest Dermatol, 1994, Vol 102, No 6, 857-861

  However, the conventional drugs that have been used to suppress excessive production of TNF-α as described above are not preferable for human health because of the problem of side effects. Therefore, there is a need for an edible composition that does not cause such problems and has an action that can significantly promote the production and release of a calcitonin gene-related peptide that plays an important role in the suppression of overproduction of TNF-α. ing.

  The present invention has been made in view of such circumstances, and is intended to prevent and treat gastric mucosal injury, lifestyle-related diseases, obesity, malignant tumors and osteoporosis, to reduce organ transplant rejection, and to invasive factors such as infection. An object of the present invention is to provide an edible composition using natural ingredients that have an effect of preventing and reducing organ damage associated with a biologically invasive reaction, a hair-growth effect, and that are gentle to the human body and have no side effects.

  As a result of intensive studies to achieve the above-mentioned object, the present inventors have found that the production and release of calcitonin gene-related peptide are significantly promoted by combining isoflavone and capsaicin, and the present invention has been completed. It was.

That is, the gist of the present invention is the following edible composition having the following calcitonin gene-related peptide production and release promoting actions:
(1) An edible composition having isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(2) An edible composition for preventing and treating gastric mucosal injury, comprising isoflavones and capsaicin as essential components, and having a calcitonin gene-related peptide production and release promoting action.
(3) An edible composition for the prevention and treatment of lifestyle-related diseases, comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(4) An edible composition for the prevention and treatment of obesity, comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(5) An edible composition for malignant tumor prevention, recurrence prevention and treatment, comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(6) An edible composition for prophylaxis and treatment of osteoporosis, comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(7) An edible composition for reducing organ transplant rejection, comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(8) An edible composition for preventing and alleviating organ damage associated with a biological invasive reaction to invasive factors such as infection, trauma, and surgery, having isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(9) An edible composition for hair growth comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.
(10) The edible composition according to any one of (1) to (9), wherein the isoflavone is soybean-derived isoflavone.
(11) The edible composition according to any one of (1) to (10), wherein the capsaicin is capsaicin derived from chili.

As a result of activation of sensory nerves stimulated by capsaicin, one of the pungent components of chili pepper, capsaicin, in the water immersion restraint stress gastric mucosal lesion model, The calcitonin gene-related peptide is released and the production of TNF-α is suppressed, and at the same time, vascular endothelial NO synthase (eNOS) is activated, and prostaglandins such as PGI ₂ and PGE ₂ are activated through enhanced NO production. We have previously found that production of gin and insulin-like growth factors is enhanced. This mechanism of action is used to prevent and treat lifestyle-related diseases such as gastric mucosal injury, diabetes, hypertension, hyperlipidemia, and arteriosclerosis, obesity, malignant tumors, osteoporosis, and reduction of rejection after organ transplantation. It is effective for prevention and reduction of organ damage associated with biological invasive reaction to various invasive factors, and for hair growth in male pattern hair loss.

  As a result of further studies based on such findings, the present inventors have mainly studied daidzein, genistein, glycitein, soybean in, genistin, glycitin, 6 ″ -O-malonyl soybean in, "-O-malonyl genistin, 6" -O-malonyl glycitin, 6 "-O-acetyl soybean in, 6" -O-acetyl genistin, 6 "-O-acetyl glycitin and 6" -O found in natto -Isoflavones such as succinyl soybean in, 6 "-O-succinyl genistin and 6" -O-succinyl glycitin increase the production of nerve growth factor (NGF), thereby increasing the calcitonin of capsaicin-sensitive sensory neurons The production-promoting action of gene-related peptides was significantly enhanced, and the production of capsaicin increased. The release of the tonin gene-related peptide was promoted, and as a result, it was found that TNF-α production suppression, NO production enhancement, prostaglandin production, and insulin-like growth factor production were also promoted, and the present invention was completed. Is.

  The edible composition comprising isoflavones and capsaicin as essential components of the present invention not only has an effect of preventing and treating gastric mucosal injury by promoting production and release of calcitonin gene-related peptide, but also diabetes, hypertension. Life-style related diseases such as hyperlipidemia and arteriosclerosis, obesity, prevention of malignant tumor occurrence and recurrence prevention, prevention and treatment of osteoporosis, reduction of rejection after organ transplantation, infection, trauma, surgery, etc. It is effective for preventing or treating organ damage due to various invasive factors, and for hair growth. In addition, the edible composition having calcitonin gene-related peptide production and release promoting action of the present invention is composed of capsaicin contained in chili peppers that have been used for many years as a food and isoflavone contained in soybeans as active ingredients. There is no problem with sex, and there are advantages that its action is mild and there are no side effects.

  Next, embodiments of the present invention will be described in detail.

  As described above, the edible composition of the present invention is characterized by a combination of isoflavone and capsaicin, and contains these components as essential components. Such a configuration significantly promotes the production and release of calcitonin gene-related peptides. Here, the essential component refers to an optional component, which is a component that is necessarily contained in terms of configuration, and is not subject to quantitative restrictions.

  The origin of the isoflavones used in the present invention is not particularly limited, and examples thereof include soybean seeds, kuzu roots and foods using them. Among them, the hypocotyl of soybean is convenient because it contains a large amount of isoflavone. It is also possible to use foods such as natto.

  The isoflavones include daidzein, genistein, glycitein, soybean in, genistin, glycitin, 6 "-O-malonyl soybean in, 6" -O-malonyl genistin, 6 "-O-malonyl glycitin, 6" -O-acetyl soybean. 6 "-O-acetylgenistine, 6" -O-acetylglycistin, 6 "-O-succinyl soybean in, 6" -O-succinylgenistin, 6 "-O-succinylglycitin and puerarin In addition, the above-mentioned various isoflavones are used alone or in combination of two or more in the present invention.

  Since isoflavones with high concentrations are used, it is also possible to use extracts such as soybeans, soybean foods, soybean hypocotyls, kudzu roots, and purified products thereof. The solvent for extracting isoflavones is not particularly limited, but it is preferable to use water or alcohol such as ethanol. The purification method can use a synthetic adsorbent, an ion exchange resin, ultrafiltration, or the like, but is not particularly limited. The extract or purified solution may be used as it is, but a concentrated solution obtained by concentrating them, or a powdered product after drying the extract or purified solution may be used.

  The origin of capsaicin used in the present invention is not particularly limited, but it is desirable to use chili.

  As capsaicins contained in chili, capsaicin, dihydrocapsaicin, nordihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin are known. The various capsaicins described above are used alone or in combination of two or more in the present invention.

  As for the chili pepper, a pulverized and powdered one can be used as it is. However, since capsaicin having a high concentration is used, an industrially produced chili extract can be used. Pepper extract extracted with water-containing ethanol, chili pepper oleoresin extracted mainly with non-polar to intermediate polar organic solvents such as hexane and ether, and red pepper oleoresin again extracted with ethanol, and insoluble matter was filtered out. Etc. can be used according to their characteristics.

  The edible composition of the present invention contains the isoflavone and capsaicin obtained as described above as essential components, and a mixture of these may be used directly as it is. Alternatively, a solution dissolved or dispersed in an appropriate liquid carrier or mixed in an appropriate powder carrier is used. The content of the isoflavone that can be an active ingredient is usually 0.001 to 0.5% by weight (hereinafter abbreviated as “%”) for a liquid, and in the case of a powder. It is preferable that it is in the range of 0.01 to 80%, and in the case of granules, it is preferable that it is in the range of 0.01 to 40%. In addition, the content of capsaicin that can be an active ingredient is usually preferably 0.0001 to 0.05% in the case of a liquid agent, and 0.0001 to 5% in the case of a powder. In the case of a granule, it is suitable in the range of 0.0001 to 5%. And in this invention, the ratio of the said isoflavone and capsaicin is important, It is preferable to contain by the weight ratio of isoflavone: capsaicin = 5000: 1 to 1:10, More preferably, isoflavone: capsaicin = 500 : The range of the weight ratio of 1-1: 1. That is, when both are contained in such a ratio, the synergistic effect of both enables the calcitonin gene-related peptide production and release promoting action required in the present invention to be effectively obtained.

  The edible composition of the present invention is expected not only for humans but also for animals such as pets and livestock, as well as the same effects as those described above when administered to humans. And the dose depends on conditions such as the difference in organisms to be administered, the difference between the above-mentioned various effects and the purpose of obtaining, the sex, weight, age, etc. of the administered person. Is set as appropriate. For example, for an adult, the above-mentioned mixture of isoflavone and capsaicin can be administered in several divided doses so as to be 10 to 400 mg per day. In animals such as pets and livestock, the above mixture is preferably contained in the animal feed in a range of 0.001 to 5% and administered in a range of 1 to 400 mg / kg body weight per day.

  The edible composition having the calcitonin gene-related peptide production and release-promoting action of the present invention is not particularly limited as long as it contains isoflavone and capsaicin as active ingredients as described above. Specifically, in addition to general foods such as beverages, cooked foods, desserts, confectionery, dairy products, noodles, breads, preferably solids and semi-solids such as tablets, powders, granules, etc. it can.

  Optional ingredients other than isoflavones and capsaicin include, for example, seasonings such as sweeteners, salting agents, acidulants, umami, emulsifiers, dispersants, antioxidants, preservatives, thickeners, binders, and fragrances. can give. In the case of tablets, powders, granules, etc., excipients, binders, disintegrants, film agents, lubricants, extenders, diluents, pH adjusters, emulsifiers, dispersants, stabilizers, Examples include miso odorants and colorants.

  Hereinafter, the present invention will be specifically described with reference to examples.

[Test of calcitonin gene-related peptide production and release promotion and gastric mucosal injury inhibition by isoflavones and capsaicin in a water-immersion restraint stress gastric mucosa lesion model]
Wistar male rats (280 to 320 g, 8 per group) divided into 5 groups were fasted for 24 hours, and then 4 groups were restrained for 8 hours by a stress gauge installed in a water bath with a water temperature of 22 ° C. (water Immersion restraint group). After restraint, all groups of rats were sacrificed and the stomach was removed.

  Here, as shown in Table 1 below, the above five groups are classified into the isoflavone administration group, the capsaicin administration group, the isoflavone + capsaicin administration group, and the non-administration group (normal diet only) from the feed administered before the fasting. Belonging to one. That is, in the isoflavone administration group, breeding was carried out with a feed supplemented with soybean germ extract (Fujiflavone P10, manufactured by Fujicco Co., Ltd.) so that the intake of isoflavone was 90 mg / kg body weight / day from 28 days before the water immersion restraint treatment. . In the capsaicin administration group, capsaicin was dissolved in a 10% ethanol aqueous solution containing 10% Tween 20, and orally administered 1 hour before water immersion restraint (administration of 1 mg / kg body weight as capsaicin). Furthermore, in the isoflavone + capsaicin administration group, both were administered in the same manner as described above.

  Then, the above five groups, that is, a normal group (normal; no water immersion restriction), a control group (Control; water immersion restriction), and three test groups (water immersion restriction + isoflavone, water immersion restriction + capsaicin, water immersion restriction + Based on the stomach excised from isoflavone + capsaicin), the gastric injury index and gastric calcitonin gene-related peptide concentration (CGRP) were measured. These results are also shown in Table 1 below. In addition, each data shows the average value in each group.

  From the results of Table 1 above, it can be seen that simultaneous intake of isoflavone and capsaicin promotes the production of calcitonin gene-related peptide in the stomach and can prevent and treat gastric mucosal injury.

[Administration study for gastric ulcer patients]
Targeting patients with gastric ulcers observed by endoscope, the group was divided into 4 groups, 4 people per group. In Group 1, 40 mg of isoflavone is ingested daily by administration of a tablet containing isoflavone (isoflavone extract; using Fujiflavone P40 manufactured by Fujikko Co.), and in Group 2, 1 is administered by administration of a tablet containing capsaicin. 1 mg of capsaicin was ingested daily. Group 3 was administered with a tablet containing both, and Group 4 was administered a pseudo tablet. This was done for 20 days.

  Then, while confirming subjective symptoms during the administration period, an endoscopic examination of the stomach was performed after the administration. The results are also shown in Table 2 below. Each data represents an average value in each group.

  From the results of Table 2 above, a significant improvement effect on gastric ulcer was observed by taking isoflavone and capsaicin simultaneously.

[Evaluation of the effect of isoflavones and capsaicin on the production of calcitonin gene-related peptide and its effect on osteoporosis in ovariectomized rats]
80 days old SD female rats divided into 5 groups (6 per group. Four groups out of the above 5 groups were rats on the 10th day after the extraction). [Calcium deficient diet (Ca: 0.004%, P: 0.3%)] and the like were administered, and the animals were reared for 28 days. Thereafter, the rats were fasted overnight (18 hours), the rats of all groups were sacrificed, and the femurs were removed therefrom.

  Here, as shown in Table 3 below, the above five groups are divided into the isoflavone administration group, the capsaicin administration group, the isoflavone + capsaicin administration group, and the non-administration group (standard diet only) from the feed administered before the fasting. Belonging to one. That is, in the isoflavone administration group, in addition to the standard diet, soybean in suspended in a 1% hydroxypropylcellulose aqueous solution was orally administered (administered so that the intake of soybean in would be 90 mg / kg body weight / day) during the breeding period. In the capsaicin administration group, in addition to the standard diet, capsaicin dissolved in 10% ethanol aqueous solution containing 10% Tween 20 was orally administered during the breeding period (administered so that the capsaicin intake was 1 mg / kg body weight / day). )did. Furthermore, both were administered in the isoflavone + capsaicin administration group.

  And from the above five groups, that is, normal group (normal; no ovariectomy), control group (Control; ovariectomy) and three test groups (ovariectomy + isoflavone, ovariectomy + capsaicin, ovariectomy + isoflavone + capsaicin) Based on the removed femur, its wet weight was measured, and its volume was measured with a pycnometer to calculate the bone density. In addition, calcitonin gene-related peptide concentration (CGRP) in bone tissue was also measured. These results are also shown in Table 3 below. In addition, each data shows the average value in each group.

  From the results in Table 3 above, it can be seen that simultaneous intake of isoflavone and capsaicin promotes the production and release of calcitonin gene-related peptide and can prevent and treat osteoporosis.

[Production promotion of calcitonin gene-related peptide by isoflavones and capsaicin and its effect on hypertension in stroke-prone spontaneously hypertensive rats (SHR-SP)]
18-week-old male SHRSP / lzm rats were divided into 4 groups of 6 per group. And group 1 of them was reared with a feed supplemented with soybean germ extract (Fujiflavone P40) so that the intake of isoflavone was 90 mg / kg body weight / day. Group 2 was reared with a feed prepared so that the amount of capsaicin was 1 mg / kg body weight / day. Group 3 received both isoflavones and capsaicin. Group 4 served as a control group.

  On the 50th day after administration, the blood pressure was measured at 260 ± 5.2 mmHg in group 1, 268 ± 10.4 mmHg in group 2, 246 ± 4.4 mmHg in group 3, and 279 ± 3.1 mmHg in group 4 (group 3 is significantly different from group 4). Group 4 also had the highest measured value of calcitonin gene-related peptide in blood. Thus, it can be seen that simultaneous administration of isoflavone and capsaicin is effective for hypertension because it promotes the production of calcitonin gene-related peptide.

[Test of obesity prevention by suppression of appetite and production of calcitonin gene-related peptide by isoflavones and capsaicin in ovariectomized rats]
80-day-old SD female rats divided into 5 groups (7 rats per group. 4 groups out of the 5 groups were rats that had been ovariectomized and 10 days after excision) for 28 days. Raised. During the breeding period, feed was freely consumed. However, as shown in Table 4 below, the feed given to each of the above groups was a standard diet [calcium-deficient diet (Ca: 0.004%, P: 0.3%)], isoflavone (standard diet with isoflavone 0). Feed with 25% added), capsaicin (feed with 0.005% capsaicin added to the standard diet), isoflavone + capsaicin (feed with standard diet supplemented with 0.25% isoflavone and capsaicin 0.005%) Either.

  Then, the above five groups, that is, a normal group (normal; no ovariectomy), a control group (control; ovariectomy), and three test groups (ovariectomy + isoflavone 0.25% added feed, ovariectomy + capsaicin 0.005) % Supplemented feed, ovariectomized + isoflavone 0.25% + capsaicin 0.005% added feed), and the average food intake and final body weight were measured. These results are also shown in Table 4 below. In addition, each data shows the average value in each group.

  From the results of Table 4 above, it can be seen that simultaneous intake of isoflavone and capsaicin can promote the production and release of calcitonin gene-related peptide, suppress appetite, and prevent and treat obesity.

[Production promotion effect of calcitonin gene-related peptide by isoflavone and capsaicin in liver metastasis model prepared by transplanting colon cancer cell high metastasis strain and test for malignant tumor effect]
Liver metastasis models were created by transplanting human colon cancer cell high-metastasis strains into all spleens of predetermined rats divided into 4 groups (5 per group). On day 10 after cancer cell transplantation, ischemia / reperfusion of the liver was performed, and the number of metastasized cancer cells was counted thereafter.

  By the way, from the 28th day before the transplantation of cancer cells, group 1 out of the above 4 groups was bred with a diet supplemented with soybean germ extract (Fujiflavone P40) so that the intake of isoflavone was 90 mg / kg body weight / day. Group 2 was reared with a feed prepared so that the intake of capsaicin was 1 mg / kg body weight / day. Group 3 received both isoflavone and capsaicin, and group 4 served as a control group.

  And as a result of measuring as mentioned above, although the group 1 and 2 had few metastatic cancer cell numbers compared with the group 4, it was not a significant difference. In contrast, in Group 3, metastasis was significantly suppressed. The amount of calcitonin gene-related peptide was also higher than that of other groups. Thus, it can be seen that simultaneous administration of isoflavone and capsaicin promotes the production of calcitonin gene-related peptide and is effective for metastasis of malignant tumors.

[Production promotion of calcitonin gene-related peptide by isoflavones and capsaicin and its effect on hypertension in a rat liver transplantation model]
Livers excised from all predetermined rats divided into 4 groups (5 per group) were stored in organ preservation solution for 24 hours, and then transplanted to syngeneic rats. Thus, at the same time as determining the survival rate, peripheral blood lymphocytes were separated, and the cell surface molecules were analyzed by FACS.

  By the way, from the 28th day before liver transplantation, group 1 out of the above 4 groups was bred with a feed supplemented with soybean germ extract (Fujiflavone P40) so that the amount of isoflavone was 90 mg / kg body weight / day. Were fed with a feed prepared so that the capsaicin intake was 1 mg / kg body weight / day. Group 3 received both isoflavone and capsaicin, and group 4 served as a control group.

  And as a result of experimenting as described above, Group 1 and Group 2 had slightly higher growth rates than Group 4. On the other hand, it was found that group 3 was clearly improved in the survival rate and was in a state of tolerance for transplantation. The amount of calcitonin gene-related peptide was also clearly higher than other groups. This shows that simultaneous administration of isoflavone and capsaicin promoted the production of calcitonin gene-related peptide and reduced organ transplant rejection.

[Test of production of calcitonin gene-related peptide by isoflavone and capsaicin and effect on blood pressure reduction for endotoxin shock model]
Endotoxin 5 mg / kg body weight was intravenously injected into all predetermined rats divided into 4 groups (5 per group), a shock model was created, and blood pressure was measured.

  By the way, from 28 days before endotoxin administration, group 1 out of the above 4 groups was bred with a feed supplemented with soybean germ extract (Fujiflavone P40) so that the amount of isoflavone was 90 mg / kg body weight / day. Were fed with a feed prepared so that the capsaicin intake was 1 mg / kg body weight / day. Group 3 received both isoflavone and capsaicin, and group 4 served as a control group.

  And as a result of measuring as mentioned above, compared with the group 4, the fall of the blood pressure of the groups 1 and 2 was suppressed a little. On the other hand, Group 3 showed a remarkable blood pressure lowering suppressing effect. The amount of calcitonin gene-related peptide was also clearly higher than other groups. Thus, it can be seen that simultaneous administration of isoflavone and capsaicin promoted the production of calcitonin gene-related peptide and suppressed the excessive bioinvasive reaction to infection.

[Improvement of calcitonin gene-related peptide and insulin-like growth factor in hair follicles by isoflavones and capsaicin and assay of hair-growth effect]
C3H / He mice purchased at 3 weeks of age were randomly divided into 4 groups of 10 mice per group, and after 1 week of acclimatization, group 1 had an isoflavone intake of 90 mg / kg body weight / day. The group 2 was reared with a feed supplemented with soybean germ extract (Fujiflavone P40), and the group 2 was reared with a feed prepared so that the capsaicin intake was 2 mg / kg body weight / day. Group 3 received both isoflavone and capsaicin, and group 4 served as a control group. Then, the hair was cut at 6 weeks of age, and the subsequent hair growth was observed. That is, the number of mice whose hair regeneration was completed within 40 days after the start of intake of the test feed was measured. The results are shown in Table 5 below.

  From the results of Table 5 above, it was confirmed that there was a significant hair-growth effect by simultaneously taking isoflavones and capsaicin. In addition, as a result of measuring the amounts of calcitonin gene-related peptide and insulin-like growth factor in the hair follicle, it was found that group 3 to which isoflavone and capsaicin were simultaneously given was significantly more than groups 1, 2, and 4.

  The edible composition comprising isoflavones and capsaicin as essential components of the present invention has an action to suppress the production of inflammatory cytokines such as TNF-α, which is enhanced by social psychological or physical stress, and an insulin-like growth factor. By promoting the production of calcitonin gene-related peptide that induces gastric mucosa injury, lifestyle-related diseases, obesity, prevention of malignant tumor occurrence and recurrence prevention, osteoporosis prevention and treatment, after organ transplantation It has the effect of reducing the rejection reaction, prevention and treatment of organ damage due to invasive reaction after infection, surgery, and trauma, and also the effect of hair growth such as hair. In addition, it is mild in action, has no side effects and is highly safe. Such an edible composition of the present invention is expected to be of great use in preventing or treating diseases that are problematic in modern life, or overcoming physical complexities.

Claims (11)

  An edible composition comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.   An edible composition for preventing and treating gastric mucosal injury, comprising isoflavones and capsaicin as essential components and having calcitonin gene-related peptide production and release promoting action.   An edible composition for the prevention and treatment of lifestyle-related diseases, characterized by comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.   An edible composition for the prevention and treatment of obesity, comprising isoflavones and capsaicin as essential components and having calcitonin gene-related peptide production and release promoting action.   An edible composition for malignant tumor prevention, recurrence prevention and treatment, characterized by having isoflavones and capsaicin as essential components, and having calcitonin gene-related peptide production and release promoting action.   An edible composition for the prevention and treatment of osteoporosis, comprising isoflavones and capsaicin as essential components, and having a calcitonin gene-related peptide production and release promoting action.   An edible composition for reducing organ transplant rejection, characterized by comprising isoflavones and capsaicin as essential components and having calcitonin gene-related peptide production and release promoting action.   An edible composition for the prevention and reduction of organ damage associated with biologically invasive responses to invasive factors such as infection, trauma, and surgery, characterized by isoflavones and capsaicin as essential components, and has a calcitonin gene-related peptide production and release promoting action Stuff.   An edible composition for hair growth, comprising isoflavones and capsaicin as essential components and having a calcitonin gene-related peptide production and release promoting action.   The edible composition according to any one of claims 1 to 9, wherein the isoflavone is soybean-derived isoflavone.   The edible composition according to any one of claims 1 to 10, wherein the capsaicin is chili-derived capsaicin.