JP2005000080A - Method for producing low protein cereal and low protein cereal obtained by the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide low protein cereal having well-balanced nutritive value and easily obtainable for diet therapy for kidney disease patients, and to provide a method for producing the low protein cereal. <P>SOLUTION: The method for producing the low protein cereal comprises a labeling process H of labeling PB-I and PB-II by antigen-antibody reaction among protein body patterns of storage protein contained in cereal and observing the PB-I and PB-II, an image processing process G of capturing images labeled by the labeling process H to compute a percent occupied by the PB-I and PB-II and localized positions thereof, and determining the shape of flat polished rice and the percentage of polished rice from the percent occupied by PB-I and PB-II and the localized positions thereof computed by the image processing process G. Furthermore, the method comprises a rice polishing process S of selecting the abrasive grain of a rice polishing machine according to the shape of the flat polished rice, and controlling peripheral velocity and pressure to remove PB-II at high ratio from the storage protein contained in cereal. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a low protein grain and a method for producing the same, for example, as a diet for kidney disease patients who need to restrict protein intake.
[0002]
[Prior art]
Among grains, rice is a staple food for Japanese people and is an important protein source, but most of the rice storage protein is occupied by protein called prolamin and protein called glutelin. PB-I ”and protein body II (hereinafter referred to as“ PB-II ”) are known (see, for example, Non-Patent Document 1).
[0003]
[Non-Patent Document 1]
Kunisuke Tanaka, Shoji Fujii, “Rice Protein Body”, Journal of the Japan Brewing Society, Vol. 84, No. 11, 1989
In recent years, development of low protein rice for use in dietary therapy for kidney disease patients who need to restrict protein intake is expected. This low-protein rice has been artificially adjusted for the content of rice storage protein. Conventionally, the development of low-glutelin rice with reduced digestible glutelin and increased prolamin that is difficult to digest by breeding by mating, (For example, refer nonpatent literature 2), development of what remove | eliminated only the protein of a specific molecular weight by carrying out the salt water treatment of the conventional low protein rice (refer patent document 1) is performed.
[0005]
[Non-Patent Document 2]
National Agricultural Research Organization, Kinki Chugoku Shikoku Agricultural Research Center,
Delicious rice that can reduce protein intake, [Search May 27, 2003], Internet <URL: http: // www. naro. affrc. go. jp / sakukai / ineiku / Lgcsoft. html>
[Patent Document 1]
Japanese Patent No. 3055729 [0006]
However, in low-glutelin rice developed by breeding by the above-mentioned crossing, it is necessary to spend a lot of time on breeding based on abundant knowledge and experience, and respond quickly to consumer needs There is a problem that cannot be done. On the other hand, special knowledge such as biochemistry is required even if the protein of a specific molecular weight is removed by treating low protein rice with the above salt solution, and low protein rice can be obtained by simple operations. It is difficult.
[0007]
Therefore, in order to easily obtain low-glutelin rice, the rice is polished as usual and the protein is mainly removed by the polished rice (for example, see Patent Document 2).
[Patent Document 2]
Japanese Patent Laid-Open No. 7-170922
According to paragraph 0009 of this publication, “Brown rice contains 7 to 8% of protein, but even if it is polished, the decrease in protein is slow, and even 70% of polished rice contains 4 to 5%. The main component of the protein in the endosperm is glutelin, which is in the vicinity of the starch double grain in the form of protein body.The ratio of polished rice of sake brewing suitable rice (Hyogo Yamada Nishiki) is about 70-60% As a result, the content of crude protein is reduced to about ½ the amount of general rice (produced in Chiba Prefecture). ”By improving the rice polishing ratio, the crude protein contained in rice is reduced. It is well known.
[0009]
However, in the rice milling method considering only the rice milling ratio as described above, there is a concern that easily digestible glutelin is removed and prolamin which is difficult to digest is also removed, resulting in a substantial decrease in protein. However, there is a problem that the balance of nutritional value becomes worse.
[0010]
[Problems to be solved by the invention]
In view of the above problems, it is a technical object of the present invention to provide a low protein cereal having a good balance of nutritional value and easily obtainable for dietary therapy for patients with kidney disease and a method for producing the same.
[0011]
[Means for Solving the Problems]
In order to solve the above-mentioned problems, the invention of claim 1 is directed to labeling PB-I and PB-II by antigen-antibody reaction among protein body types of storage proteins contained in cereals, and to label PB-I and PB-II. A labeling step to be observed, an image processing step for calculating the proportion of the PB-I and PB-II and the uneven distribution position of the image labeled by the labeling step, and the PB- calculated by the image processing step Determine the shape and milling ratio of flat milling from the proportion of I and PB-II and the uneven position, and select the grain of the milling mill, control the peripheral speed and pressure according to the shape of the flat milling, And a scouring process for removing PB-II at a high ratio from the storage protein contained in the product.
[0012]
Thereby, in the labeling step and the image processing step, the ratios occupied by PB-I and PB-II and the uneven distribution positions thereof are calculated, and the grain is flattened using the feature amount data of PB-II. Therefore, compared with the conventional method of improving the rice milling rate and reducing the protein of rice based on the intuition and experience of the operator, the shape data and the rice milling rate data of flat rice are determined and the rice milling machine is controlled. Therefore, it becomes possible to remove excessive protein at a high ratio by preventing excessive sperm and insufficient sperm.
[0013]
The labeling step includes a thin-section preparation step for preparing a grain sample into a specimen for a microscope, and the specimen sample obtained in the thin-section preparation step is stained by staining PB-I and PB-II with an antibody. And a microscopic observation step of observing PB-I and PB-II stained and labeled by the antigen-antibody reaction step with a fluorescence microscope. As a result of observation, PB-I and PB-II are clearly labeled by color.
[0014]
The image processing step includes an image capturing step for capturing the image labeled in the labeling step, an image recording step for recording a plurality of images captured in the image capturing step, and a storage in the image recording step. An image processing step of performing two-dimensional imaging processing or three-dimensional imaging processing from a plurality of obtained images, and an area or volume occupied by PB-I and PB-II of storage proteins contained in the grain in the image processing step And a PB-II feature amount calculating step for calculating the uneven distribution position of PB-II in the rice grain, so that the amount of PB-II to be removed is calculated in more detail using a three-dimensional image. In addition, when a two-dimensional image is selected, the amount of processing to a computer is small and processing in a short time is possible.
[0015]
Furthermore, the proportion of PB-II in which digestible glutelin is accumulated from the storage protein contained in the cereal and the uneven distribution position thereof are calculated, and obtained by removing PB-II from the storage protein at a high ratio using flat rice. Since it is a low protein grain, unnecessary protein is removed and the problem of poor nutritional balance can be solved.
[0016]
Moreover, even if it is rice or wheat as a grain, it is applicable.
[0017]
DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention will be described with reference to the drawings. In addition, although rice is demonstrated as an example as a grain, it is not limited to this, Wheat can also be applied as a grain. FIG. 1 is a block diagram showing a method for producing low-protein rice of the present invention, in which a labeling step H for labeling PB-I and PB-II of a protein body type of rice storage protein by an antigen-antibody reaction; Image processing step G that takes in the image labeled in the labeling step H and calculates the proportion of the PB-I and PB-II and the uneven distribution position thereof, and the characteristics of the PB-II calculated in the image processing step G The shape of the flat milled rice is determined from the amount, and the milling process S for removing PB-II at a high ratio is the main process.
[0018]
The labeling step H will be described below. First, when brown rice serving as a raw material is received (step S1), a part (for example, 10 grains) is collected as a brown rice sample (step S2) and provided to the labeling step H. In the labeling step H, a thin slice is prepared in order to prepare the supplied brown rice sample as a specimen for a microscope (step H1). The preparation of this thin slice will be described with reference to FIGS. FIG. 2 is a schematic view showing the internal structure of brown rice. For example, one of 10 brown rice samples is cut along the line aa ′ in FIG. 2 and the other is cut along the line bb ′. And cut along the cc ′ line and the AA ′ line in the same manner to create a total of four sample pieces. This sample piece may be cut by hand using a single-edged razor or the like, and the sample at one location aa ′ line or the length of the rice grain (major axis) that becomes the thickness of the rice grain (minor axis) Only a sample at one place along the line AA ′ may be created.
[0019]
Next, the aa ′ line sample piece, the bb ′ line sample piece, the cc ′ line sample piece, and the AA ′ line sample piece cut with a single-blade razor or the like are sampled. FIG. 3 is a process diagram for preparing a specimen of a brown rice sample, but the cut sample piece (see FIGS. 3A and 3B) 10 is an acrylic slide with its cut surface 11 down. It is fixed on the glass 12 (see FIG. 3C). When fixing, first, the surface of the acrylic slide glass 12 is washed with a 70% ethanol solution, 10 to 40 μl of an instantaneous adhesive is dropped on the slide glass 12, and the cut surface 11 of the cut sample piece 10 is placed below. Then, place it on the instantaneous adhesive droplet and adjust the direction with tweezers. When the sample piece 10 is pushed from above and firmly fixed on the slide glass 12, the specimen sample 13 is completed.
[0020]
When the specimen sample 13 is completed, the bottom surface of the slide glass 12 is wiped off to be cleaned and placed on a stage of a sliding microtome (an improved version of the model HR400R manufactured by MICROM) to cut out a smooth surface. FIG. 4 is a perspective view showing a schematic structure of a sliding microtome. The sliding microtome 20 is a device that cuts a biological tissue into a thin sample as a microscope specimen. The sliding microtome 20 cuts a tissue mass into a thin piece of several μm with a microtome sword 22 having a sharp blade 21. In order to closely fix the specimen 13, the gap 24 between the stage 23 and the slide glass 12 may be set to a negative pressure. For example, air may be sucked out by a pump (not shown). If it is 3 or more, the slide glass 12 and the stage 23 are sufficiently tightly fixed, and if the pump meter is 3 or less, a foreign object is sandwiched between the slide glass 12 and the stage 23. 12 and the stage 23 may be used as a guideline.
[0021]
When it is confirmed that the slide glass 12 and the stage 23 are fixed in close contact with each other, a coarse-feeding hand wheel (not shown) is adjusted to bring the blade 21 of the microtome 20 closer to the sample piece 10 and the section thickness. By adjusting the setting handwheel 25, for example, the section thickness is set in the range of 20 to 30 μm. When the setting is completed, the microtome sword 22 is moved horizontally toward the specimen sample 13, and cutting is performed in the manner of cutting with a plane. When the cutting of the specimen 13 is repeated several times and the cutting surface approaches an appropriate position, the section thickness is reset to 10 μm, the smooth surface 26 is created at a suitable position, and the operation is completed.
[0022]
Next, the antigen-antibody reaction of the specimen sample 13 is performed (step H2 in FIG. 1). The antigen-antibody reaction will be described with reference to FIG. FIG. 5 is a schematic view showing the inside of the antigen-antibody reaction container. First, the buffer solution 1X TBST + BSA is dropped on the smooth surface 26 of the specimen sample 13 on the slide glass 12 to perform blocking for preventing disturbance. Next, this specimen sample 13 is put into a container 31 that can be sealed with a lid 30 shown in FIG. 5, and blocking is performed so as not to evaporate. At this time, a paper towel 32 soaked in water is laid on the bottom of the container 31, and a parafilm 33 is placed thereon. In this state, a staining solution (ATTO dyed Taro program 1) is added under the conditions of 37 ° C. and 1 hour.
[0023]
And the following processes are performed in order to carry out the antibody reaction of glutelin among rice storage proteins. First, washing was performed twice with buffer 1X TBST, mouse-derived anti-glutelin antibody (100-fold dilution: 1X TBST + BSA) was dropped as a primary antibody onto the smooth surface 26, and the staining solution (37 ° C., 1-2 hours) Add ATTO dyed Taro program 3).
[0024]
Further, washing was performed 3 times for 10 minutes with buffer solution 1X TBST, Rhodamine-labeled anti-mouse IgG antibody (200-fold dilution: 1X TBST + BSA) was dropped on the smooth surface 26 as a secondary antibody, and conditions of 37 ° C. and 1-2 hr Add the staining solution (ATTO Dye Taro Program 3).
[0025]
Next, the following steps are performed in order to cause an antibody reaction with prolamin in the rice storage protein. First, washing was performed 3 times for 10 minutes with buffer 1X TBST, and a rabbit anti-derived 16 kDa prolamin antibody (100-fold diluted: 1X TBST + BSA) was dropped on the smooth surface 26 as a primary antibody, and the conditions were 37 ° C. and 1-2 hr. Add staining solution (ATTO Dye Taro Program 3).
[0026]
Further, washing was performed 3 times for 10 minutes with buffer 1X TBST, and FITC-labeled anti-rabbit IgG antibody (200-fold diluted: 1X TBST + BSA) was dropped on the smooth surface 26 as a secondary antibody, and conditions of 37 ° C. and 1-2 hr Add the staining solution (ATTO Dye Taro Program 3) to complete the antigen-antibody reaction.
[0027]
In Step H3 of FIG. 1, the specimen sample 13 is observed with a confocal laser scanning fluorescence microscope. For this observation, a laser light source connection microscope (for example, an inverted epifluorescence microscope IX 70, bottom TV port IX-TVR, manufactured by Olympus) and a CCD camera (for example, CCD CAMERA CS3450, manufactured by teli) are used. Necessary. In addition, a video monitor and computer are required. According to the observation of the smooth surface 26 by the confocal laser scanning fluorescence microscope, for example, PB-I is clearly labeled as green and PB-II as red due to color.
[0028]
Next, an image processing process G for capturing an image of the smooth surface 26 and calculating a feature amount of PB-II will be described. FIG. 6 is a perspective view showing the image input device in the image processing step G. The smooth surface 26 of the sample piece 10 of the specimen sample 13 is photographed by the CCD camera 40, and the output is captured by the image capturing device G1. When there are a plurality of sample pieces 10 (three in FIG. 6, 10a, 10b, and 10c), the CCD camera 40 or the specimen sample 13 may be moved to image the smooth surfaces 26a, 26b, and 26c. The image captured by the image capturing device G1 is temporarily stored as a video signal in the image recording device G2. Next, the image processing means G3 (see FIG. 1) performs a two-dimensional imaging process on the image stored in the image recording device G2, or performs a three-dimensional imaging process (step G3-1 in FIG. 6). That is, when the three-dimensional imaging process (step G3-2) is selected, the next step of flat milling can be executed with higher accuracy, and the two-dimensional imaging process (step G3-3) is selected. Thus, it is possible to perform image processing in a short time.
[0029]
The three-dimensional imaging process will be described with reference to FIG. In the three-dimensional imaging process (step G3-2), each image of the smooth surfaces 26a, 26b, 26c, and 26A accumulated in the image recording device G2 is extracted and converted into three-dimensional data that can be processed by a computer such as a computer. Converted. The converted three-dimensional data is synthesized to recognize the shape of the rice grain. At this time, the image of the smooth surface 26 is clearly labeled as green PB-I and red PB-II (hatching in FIG. 7A). Even in the synthesized three-dimensional image, PB-I is clearly labeled as green and PB-II as red (hatched in FIG. 7B).
[0030]
From this three-dimensional image, the volume of PB-I and the volume of PB-II to be removed are calculated (step G3-4 in FIG. 6), and the uneven distribution position of PB-II in the rice grain (step G3- 5) is calculated, the feature amount of PB-II is obtained (step G4), and the image processing step G ends. Similarly, when the two-dimensional imaging process (step G3-3) is selected, the area of PB-I and the area of PB-II to be removed are calculated (step G3-6 in FIG. 6). The uneven distribution position of PB-II in the rice grain (step G3-5) is calculated. In this case, the amount of processing to the computer is small and processing in a short time is possible.
[0031]
Next, the rice milling process S (see FIG. 1) will be described. PB-II in the rice grain is unevenly distributed from the center of the rice grain (for example, PB-II (hatching) is unevenly distributed on the back side of the rice grain in FIG. 7), and the volume and uneven distribution of PB-I and PB-II. By knowing the position, it is possible to determine the shape of the flat milled rice and the milling ratio, and to remove PB-II at a high ratio. As shown in FIG. 8, the flat milled rice includes rod-shaped milled rice, plate-shaped milled rice, and spherical milled rice, which are appropriately selected by the control unit of the milling machine.
[0032]
The rice milling device 50 used in the rice milling process S is composed of three rice milling machines 51A, 51B, 51C having different grindstone particle sizes, and a peripheral speed, pressure, and raw material electrically connected to the rice milling machines 51A, 51B, 51C. A control unit 52 for controlling the flow rate, an input / output unit 53 for the control unit 52, a feature amount setting means 54 for setting a feature amount of PB-II calculated in the image processing step G, and a rice variety. It is provided with a kind setting means 55 for setting, a kind setting means 56 for setting whether it is sticky rice or sticky rice, and a data storage device 57 for inputting / outputting data.
[0033]
Next, a method for producing low protein rice in the above configuration will be described with a focus on the rice milling step S. Ten grains of brown rice to be polished are collected (see FIG. 1), and PB-I and PB-II of the protein body type of rice storage protein are labeled by antigen-antibody reaction in labeling step H, and image processing step G Then, an image labeled with PB-I and PB-II is taken in, and PB-II feature quantities such as PB-I, PB-II volume, and PB-II uneven distribution position are calculated in advance. In the rice milling process S, the feature amount of PB-II is input to the control unit 52 via the feature amount setting means 54.
[0034]
The rice milling machines 51A, 51B, 51C in the rice milling process S use rice milling machines for sake brewing, and supply raw materials to the rice milling machines 51A, 51B, 51C in order to perform multi-stage rice milling, or any one rice milling machine 51 The processing conditions can be controlled moderately by circulating the raw material a plurality of times and milling the rice. In addition, when the rice is polished, the control unit 52 receives the PB-II characteristic amount data (PB-II uneven distribution position data, PB-II occupying ratio data) and rice variety data (for example, Koshihikari produced in 2002) , Rice type data (for example, brown rice) is input, and flat rice shape data (for example, plate-shaped rice) and rice polishing ratio data (for example, 52.8%) are determined based on each data. Thereby, selection of the grain size of the grindstone of the rice milling machine 51, peripheral speed, pressure and flow rate are controlled.
[0035]
Since the present invention has the above-mentioned configuration, the shape data of the flat milled rice and the milled rice based on each data are compared with the conventional method of improving the rice milling ratio and reducing the protein of the rice based on the intuition and experience of the operator. Since the yield data is determined and the rice milling machine 51 is controlled, it becomes possible to remove excessive proteins at a high ratio by preventing excessive semen and insufficient semen.
[0036]
Further, if the PB-II feature amount data, the shape data of flat rice milling, and the rice milling rate data are stored in advance in the data storage device 57 for each rice variety and type, the operator can select the rice variety and type. By selecting the grain size of the grindstone from the rice milling machines 51A, 51B and 51C, the peripheral speed, pressure and flow rate are automatically controlled, so anyone can easily use brown rice as a diet for patients with kidney disease. Low protein rice can be obtained.
[0037]
[Example 1]
As a test sample, Koshihikari from Niigata produced in 2002 was used. First, by labeling PB-I and PB-II in the protein body type of rice storage protein by antigen-antibody reaction and observing under a microscope, the area ratio of PB-I and PB-II is different and the presence of PB-II It was found that the location was also unevenly distributed from the center of the rice grain (labeling step H). When image processing is performed to quantify this, the area ratio of PB-I and PB-II is 45:55, and the uneven distribution position of PB-II from the rice grain center is displaced 10% on the ventral side. (Image processing step G).
When the PB-II feature amount data “area ratio is 45:55” and “10% deviation on the ventral side” is input to the control unit of the rice mill (rice milling step S), the control unit displays “flat shape”. Has been decided to polish the rice to "Plate-shaped" and "Rice milling ratio is 52.8%". Furthermore, the control unit selects a rice mill with a grindstone particle size of 40 mesh from a plurality of rice mills, and sets the peripheral speed to 400 (m / min) and the pressure to 80 (gf / cm ² ) to start flat rice milling. It was.
After flat rice polishing, PB-I and PB-II of the protein body type of rice storage protein are labeled again by antigen-antibody reaction and microscopically observed, the result is a low protein rice from which PB-II has been removed in a high ratio It was.
【The invention's effect】
[0038]
As described above, according to the present invention, among the protein body types of storage proteins contained in cereals, PB-I and PB-II are labeled by an antigen-antibody reaction, and a label for observing PB-I and PB-II Step, an image processing step for calculating the proportion of the PB-I and PB-II and the uneven distribution position of the image labeled by the labeling step, and the PB-I and PB calculated by the image processing step -II occupies and the uneven distribution position determines the shape of the flat milling and the percentage of polished rice, and selects the grain of the milling mill, controls the peripheral speed and pressure according to the shape of the flat milling, and is included in the grain And a scouring step for removing PB-II from the stored protein at a high ratio, so that the ratio of PB-I and PB-II and the ratio of PB-II in the labeling step and the image processing step Calculating a localized position, so that the grain of the flat pearling is performed using the feature amount data of the PB-II. Therefore, compared with the conventional method of improving the rice milling rate and reducing the protein of rice based on the intuition and experience of the operator, the shape data and the rice milling rate data of flat rice are determined and the rice milling machine is controlled. Therefore, it becomes possible to remove excessive protein at a high ratio by preventing excessive sperm and insufficient sperm.
[0039]
The labeling step includes a thin-section preparation step for preparing a grain sample into a specimen for a microscope, and the specimen sample obtained in the thin-section preparation step is stained by staining PB-I and PB-II with an antibody. And a microscopic observation step of observing PB-I and PB-II stained and labeled by the antigen-antibody reaction step with a fluorescence microscope. As a result of observation, PB-I and PB-II are clearly labeled by color.
[0040]
The image processing step includes an image capturing step for capturing the image labeled in the labeling step, an image recording step for recording a plurality of images captured in the image capturing step, and a storage in the image recording step. An image processing step for performing two-dimensional imaging processing or three-dimensional imaging processing from a plurality of obtained images, and an area or volume occupied by PB-I and PB-II of storage proteins contained in the grain in the image processing step And a PB-II feature amount calculating step for calculating the uneven distribution position of PB-II in the rice grain, so that the amount of PB-II to be removed is calculated in more detail using a three-dimensional image. In addition, when a two-dimensional image is selected, the amount of processing to a computer is small and processing in a short time is possible.
[0041]
Furthermore, the proportion of PB-II in which digestible glutelin is accumulated from the storage protein contained in cereal and the uneven distribution position thereof are calculated, and obtained by removing PB-II from the storage protein in a high ratio by flat rice. Since it is a low protein grain, unnecessary protein is removed and the problem of poor nutritional balance can be solved.
[Brief description of the drawings]
FIG. 1 is a block diagram showing a method for producing low protein rice of the present invention.
FIG. 2 is a schematic view showing the internal structure of brown rice.
FIG. 3 is a process diagram for sampling a sample.
FIG. 4 is a perspective view showing a schematic structure of a sliding microtome.
FIG. 5 is a schematic view showing the inside of an antigen-antibody reaction container.
6 is a perspective view showing an image input device in an image processing step G. FIG.
FIG. 7 is an explanatory diagram when a three-dimensional image is created.
FIG. 8 is an explanatory diagram of the shape of flat polished rice.
[Explanation of symbols]
10 Sample piece 11 Cut surface 12 Slide glass 13 Specimen sample 20 Sliding microtome 21 Blade 22 Microtome sword 23 Stage 24 Gap 25 Hand wheel 26 Smooth surface 30 Lid 31 Container 32 Paper towel 33 Parafilm 40 CCD camera 50 Rice milling device 51 Rice milling machine 52 Control Unit 53 Input / output unit 54 Feature amount setting means 55 Product type setting means 56 Type setting means 57 Data storage device

Claims (8)

A labeling step of labeling PB-I and PB-II by antigen-antibody reaction among protein body types of storage proteins contained in cereals, and observing PB-I and PB-II;
An image processing step of taking the image labeled by the labeling step and calculating the proportion of the PB-I and PB-II and the uneven distribution position thereof;
The shape and the polishing ratio of flat milling are determined from the proportion of PB-I and PB-II calculated by the image processing step and the uneven distribution position, and the selection of the abrasive grains of the milling machine according to the shape of the flat milling A scouring process that controls the speed and pressure and removes PB-II from the storage protein contained in the cereal in a high proportion;
A method for producing a low protein grain comprising: The labeling step includes
Thin section making process to make a grain sample into a specimen for microscope,
An antigen-antibody reaction step in which the specimen sample obtained in the thin section preparation step is labeled by staining PB-I and PB-II with an antibody;
A microscopic observation step of observing the PB-I and PB-II stained and labeled by the antigen-antibody reaction step with a fluorescence microscope;
A method for producing a low protein grain according to claim 1. The image processing step includes
An image capturing step for capturing an image labeled by the labeling step;
An image recording process for recording a plurality of images captured by the image capturing process;
An image processing step of performing a two-dimensional imaging process or a three-dimensional imaging process from a plurality of images stored in the image recording process;
A PB-II feature amount calculating step for calculating the area or volume occupied by PB-I and PB-II of the storage protein contained in the grain in the image processing step and calculating the uneven distribution position of PB-II in the rice grain; ,
A method for producing a low protein grain according to claim 1 or 2. The method for producing a low protein grain according to any one of claims 1 to 3, wherein the grain is rice. The method for producing a low protein grain according to any one of claims 1 to 3, wherein the grain is wheat. A low protein obtained by calculating the ratio of occupying PB-II in which digestible glutelin is accumulated from the storage protein contained in cereal and the uneven distribution position thereof, and removing PB-II from the storage protein at a high ratio by flat rice grain. The low protein grain according to claim 6, wherein the grain is rice. The low protein grain according to claim 6, wherein the grain is wheat.

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