JP2004535245A5 - - Google Patents

Claims (14)

In a method for making an implantable support scaffold material for repairing or regenerating body tissue,
Certain derived from tissue selected from the group consisting of small intestine submucosa, stomach submucosa, bladder submucosa, respiratory submucosa, gastrointestinal submucosa, genital submucosa, and liver basement membrane Supplying an extracellular matrix material of
A step of physically pulverizing the extracellular matrix to form a coherent member piece of the extracellular matrix; and a freeze-drying of the coherent member piece of the extracellular matrix to form a constant open-cell type of the extracellular matrix Forming a foam of the method.   The extracellular matrix includes a naturally occurring extracellular matrix, and the step of physically crushing the extracellular matrix includes a process of physically crushing the naturally occurring extracellular matrix. Method.   Furthermore, the method of Claim 1 including the process which refine | purifies the said extracellular matrix before pulverizing the said extracellular matrix physically.   2. The method of claim 1, further comprising treating the extracellular matrix to substantially remove various glycoproteins, glycosaminoglycans, proteoglycans, lipids, non-collagenous proteins and nucleic acids.   The method according to claim 1, further comprising treating the extracellular matrix to remove substantially all substances other than collagen before physically crushing the extracellular matrix.   The method according to claim 1, wherein the step of physically pulverizing the extracellular matrix includes a process of physically pulverizing the extracellular matrix in the presence of a certain liquid.   The method according to claim 6, wherein the process of physically pulverizing the extracellular matrix in the presence of a certain liquid includes a process of suspending the extracellular matrix in the liquid.   The method according to claim 6, wherein the freeze-drying step includes a process of freeze-drying the cohesive member piece of the extracellular matrix and the liquid to form an open-cell foam of the extracellular matrix.   The physically pulverizing step physically pulverizes the extracellular matrix to form an elongated cohesive member piece of the extracellular matrix having a certain length longer than the width and thickness of each member piece. The method according to claim 1, further comprising:   A product made by the method of claim 1.   In addition, the following: certain bioactive substances, certain biologically derived substances, various cells, certain biological lubricants, certain biocompatible inorganic materials, and certain The product of claim 10 comprising at least one of the biocompatible polymers of:   The product of claim 10 wherein the open-cell foam of the extracellular matrix defines pores having a constant nominal pore size of 100 microns to 700 microns.   11. The product of claim 10, wherein the open-cell foam of the extracellular matrix defines pores having a constant nominal pore size of 300 microns to 700 microns.   The product of claim 10, wherein the open-celled foam of the extracellular matrix defines pores having a constant nominal pore size of less than 100 microns.