JP2004520812A5 - - Google Patents

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Publication number
JP2004520812A5
JP2004520812A5 JP2002522564A JP2002522564A JP2004520812A5 JP 2004520812 A5 JP2004520812 A5 JP 2004520812A5 JP 2002522564 A JP2002522564 A JP 2002522564A JP 2002522564 A JP2002522564 A JP 2002522564A JP 2004520812 A5 JP2004520812 A5 JP 2004520812A5
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Japan
Prior art keywords
oligonucleotides
nucleic acid
bound
spacers
bead sets
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JP2002522564A
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Japanese (ja)
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JP2004520812A (en
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Priority claimed from PCT/US2001/041956 external-priority patent/WO2002018659A2/en
Publication of JP2004520812A publication Critical patent/JP2004520812A/en
Publication of JP2004520812A5 publication Critical patent/JP2004520812A5/ja
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Claims (12)

ハイブリダイゼーションを通して対立遺伝子を検出するための方法であって、
種々のビーズセットに結合しているオリゴヌクレオチドに標的オリゴヌクレオチドをハイブリダイズさせて複合体を形成し、但し、種々のビーズセットに結合しているオリゴヌクレオチドはスペーサーを有する及びスペーサーを持たないオリゴヌクレオチドであり、複合体を種々の対立遺伝子の特異性に関して検定することを含む方法。A method for detecting alleles through hybridization comprising:
Hybridize target oligonucleotides to oligonucleotides bound to different bead sets to form a complex, provided that oligonucleotides bound to different bead sets have spacers and no spacers And assaying the complex for specificity of various alleles. 対立遺伝子特異的核酸フラグメントを分離することをさらに含む、請求項１に記載の方法。2. The method of claim 1 further comprising isolating allele specific nucleic acid fragments. 対立遺伝子特異的核酸フラグメントの分離が、種々のビーズセットに結合した特定多型に関するオリゴヌクレオチドを使用することを含む、請求項２に記載の方法。3. The method of claim 2, wherein the separation of allele specific nucleic acid fragments comprises using oligonucleotides for specific polymorphisms bound to different bead sets. 特定多型に関するオリゴヌクレオチドを種々のビーズセットに結合することをさらに含む、請求項１−３のいずれかに記載の方法。4. The method according to any of claims 1-3, further comprising binding oligonucleotides relating to the specific polymorphism to various bead sets. スペーサーを有する及びスペーサーを持たないオリゴヌクレオチドを種々のビーズセットに結合することをさらに含む、請求項１−４のいずれかに記載の方法。5. The method according to any of claims 1-4, further comprising binding oligonucleotides with and without spacers to different bead sets. 複対立遺伝子を含む標的核酸試料を得ることをさらに含み、各々の対立遺伝子がヘテロ配列部位の特有のセットを持つ、請求項１−５のいずれかに記載の方法。6. The method according to any of claims 1-5, further comprising obtaining a target nucleic acid sample comprising multiple alleles, each allele having a unique set of heterosequence sites. 標的核酸を増幅することをさらに含む、請求項１−６のいずれかに記載の方法。The method according to claim 1, further comprising amplifying the target nucleic acid. 標的核酸を一本鎖核酸に変性することをさらに含む、請求項１−７のいずれかに記載の方法。The method according to claim 1, further comprising denaturing the target nucleic acid into a single-stranded nucleic acid. 核酸鋳型を該鋳型に相補的な第二ビーズセットとハイブリダイズさせ、フローサイトメトリーによって核酸鋳型のハイブリダイゼーションを測定することにより鋳型の配列を確認することをさらに含む、請求項１−８のいずれかに記載の方法。9. The method of any of claims 1-8, further comprising hybridizing the nucleic acid template with a second bead set complementary to the template and confirming the sequence of the template by measuring hybridization of the nucleic acid template by flow cytometry. The method of crab. 標的オリゴヌクレオチドがＨＬＡ対立遺伝子である、請求項１−９のいずれかに記載の方法。10. A method according to any of claims 1-9, wherein the target oligonucleotide is an HLA allele. スペーサーを有する及びスペーサーを持たないオリゴヌクレオチドに結合しているビーズセットが種々のオリゴヌクレオチドと結合しており、蛍光呈色比によって同定することができる、請求項１−１０のいずれかに記載の方法。11. A bead set bound to oligonucleotides with and without spacers is bound to various oligonucleotides and can be identified by fluorescent color ratio. Method. 種々のビーズセットに結合しているオリゴヌクレオチドを含み、かかる種々のビーズセットに結合しているオリゴヌクレオチドがスペーサーを有する及びスペーサーを持たないオリゴヌクレオチドである組成物。A composition comprising oligonucleotides bound to various bead sets, wherein the oligonucleotides bound to such various bead sets are spacers and non-spacer oligonucleotides.
JP2002522564A 2000-08-30 2001-08-30 Methods for determining alleles Withdrawn JP2004520812A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22899400P 2000-08-30 2000-08-30
PCT/US2001/041956 WO2002018659A2 (en) 2000-08-30 2001-08-30 Method for determining alleles

Publications (2)

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JP2004520812A JP2004520812A (en) 2004-07-15
JP2004520812A5 true JP2004520812A5 (en) 2005-03-17

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JP (1) JP2004520812A (en)
CN (1) CN1293204C (en)
AU (1) AU2001289177A1 (en)
CA (1) CA2421078A1 (en)
WO (1) WO2002018659A2 (en)

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CA2406745C (en) 2001-11-13 2006-01-10 The Trustees Of The University Of Pennsylvania A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby
AU2002359284A1 (en) 2001-12-17 2003-06-30 The Trustees Of The University Of Pennsylvania Adeno-associated virus (aav) serotype 9 sequences, vectors containing same, and uses therefor
DK1453547T3 (en) 2001-12-17 2016-12-05 Univ Pennsylvania ADENOASSOCATED VIRUS (AAV) SEROTYPE 8 SEQUENCES, VECTORS CONTAINING THESE AND APPLICATIONS THEREOF
JP3752466B2 (en) 2002-04-24 2006-03-08 株式会社日立製作所 Genetic testing method
EP1536021A1 (en) * 2003-11-27 2005-06-01 Consortium National de Recherche en Genomique (CNRG) Method for HLA typing
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WO2005118862A2 (en) * 2004-04-30 2005-12-15 Applera Corporation Compositions, methods, and kits for (mis)ligating oligonucleotides
GB0600116D0 (en) * 2006-01-05 2006-02-15 Univ Cardiff Allele-specific sequencing
US8198509B2 (en) * 2007-08-07 2012-06-12 Monsanto Technology Llc Methods and compositions for selecting soybean plants resistant to southern root knot nematode
CN102428190B (en) * 2009-03-27 2014-02-26 生命技术公司 Methods, compositions, and kits for detecting allelic variants
JP2011072222A (en) * 2009-09-29 2011-04-14 Kitasato Otsuka Biomedical Assay Kenkyusho:Kk Method for detecting target nucleic acid
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WO2012135664A2 (en) 2011-03-31 2012-10-04 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex cold-pcr
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
EP3116900B1 (en) 2014-03-09 2020-07-08 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (otc) deficiency
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
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