JP2004229222A - Adjustment method of pixel shifting digital camera, sample for adjustment, and pixel shifting digital camera system - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method which can adjust a shift transition volume of a pixel shifting digital camera with a superior precision at a low cost and in a short time. <P>SOLUTION: This adjusting method of the pixel shifting digital camera comprises a first process which arranges a sample for adjustment having a figure which has at least two linear end edges at a prescribed initial position to an imaging means, a second process which changes its relative position by setting drive voltage to a pixel shifting means, a third process which images the sample by the imaging means, a fourth and a fifth processes which obtain horizontal direction component value and vertical direction component value of transition volume from the initial position, regarding a some point on the end edge of the imaged figure, a sixth process which repeats the processes from the second to the fifth by setting new drive voltage to the pixel shifting means, and a seventh process which obtains drive voltage by which a relative position becomes a prescribed value, from the drive voltage set to the pixel shifting means and the horizontal and the vertical direction component values of the transition volume. <P>COPYRIGHT: (C)2004,JPO&NCIPI

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a pixel shift digital camera system that captures a still image at each position by changing a relative position between an optical image and an imaging unit, and combines these images to achieve high resolution.
[0002]
[Prior art]
In a digital camera system that captures a light image of a subject with an image sensor such as a CCD, a so-called pixel shift method has been developed as a method for increasing the resolution without increasing the number of pixels of the image sensor itself.
[0003]
FIG. 32 is a diagram showing a configuration of a digital camera system in which a digital camera using the pixel shift method is applied to imaging a specimen image of a microscope.
[0004]
This digital camera system includes a digital camera head unit 71, a microscope 72, and a personal computer (PC) 73 that operates and controls the digital camera head unit 71. The optical image of the specimen placed on the stage of the microscope 72 is emitted to the digital camera head unit 71 through the output port of the microscope. The digital camera head unit 71 captures an incident light image from the microscope 72, a pixel shift mechanism 75 that changes two-dimensionally the relative position of the incident image on the CCD 74 and the CCD 74, an image sensor 74, and a pixel shift. It comprises a control processing unit 76 that drives the mechanism 75.
[0005]
Next, the operation of this digital camera system will be described.
[0006]
The pixel shift mechanism 75 applies a displacement for each n / m pitch (n and m are integers) in the horizontal direction and the vertical direction with respect to the interval (pixel pitch) between the imaging elements of the CCD 74, and the incident light image at each position. Image. Then, the resolution of the image can be increased by combining the images taken at these positions.
[0007]
For example, n = 1 and m = 2, and the initial positions in the x and y directions are respectively (1/2 pixel pitch, 0 pixel pitch), (0 pixel pitch, 1/2 pixel pitch), (1/2 pixel pitch). , 1/2 pixel pitch), and incident light images are taken at a total of four locations. Then, by synthesizing these images, an image having four times the number of pixels can be obtained. In the following description, (n / m pixel pitch, n / m pixel pitch) is described as (n / m pixel, n / m pixel).
[0008]
By the way, in the case of high resolution by the pixel shift method, if the displacement amount error is several percent, the image quality is deteriorated. In this example, when the pixel pitch of the CCD 74 is 4 μm, the displacement amount given by the pixel shift mechanism 75 is ½ of 2 μm. That is, if the tolerance of displacement is 5%, the relative displacement between the incident light image and the CCD 74 by the pixel shift mechanism 75 must be controlled with an accuracy of 0.1 μm or less.
[0009]
In order to achieve this control accuracy, the drive voltage of the pixel shift mechanism 75 is adjusted while measuring the displacement of the CCD 74 using a high-precision displacement measuring instrument or the like in the initial state. After the adjustment, the pixel shift mechanism 75 performs a driving operation with the adjusted voltage. However, since the amount of displacement that must be adjusted as described above is as small as about 0.1 μm, in the adjustment using a highly accurate displacement measuring instrument or the like, errors due to the assembly of the measuring instrument cannot be ignored. For this reason, accurate adjustment requires skill.
[0010]
Therefore, adjustment without using such a measuring instrument is required. As an adjustment method of such a pixel shift digital camera, an image obtained by calculating a displacement by a target movement amount from an image captured at an initial position of the pixel shift mechanism is obtained by calculation, and an image captured when the pixel shift mechanism is moved A technique for adjusting the movement amount so that the difference between images obtained by calculation is minimized is disclosed (for example, see Patent Document 1).
[0011]
[Patent Document 1]
Japanese Patent Laid-Open No. 9-135381.
[0012]
[Problems to be solved by the invention]
Normally, the pixel shift mechanism moves the CCD 74 in a grid pattern in the horizontal direction and the vertical direction within the imaging surface. Accordingly, the pixel shift mechanism has two mechanisms, a horizontal driving mechanism and a vertical driving mechanism.
[0013]
By the way, in the method disclosed in Patent Document 1, in order to minimize the difference between the target image and the image obtained when moving, the pixel shift operation is performed for each of the driving mechanisms in the horizontal direction and the vertical direction. It is necessary to repeat the calculation process for obtaining the difference to converge to the target accuracy. Therefore, there is a problem that the amount of calculation related to image processing becomes extremely large, and the calculation time required for convergence to the target accuracy also becomes long. Further, if this calculation is configured by hardware or the like to reduce the time, there arises a problem that the cost increases.
[0014]
The present invention has been made in view of such circumstances, and an adjustment method of a pixel shift digital camera, an adjustment sample, and a pixel shift digital camera system capable of accurately adjusting the shift displacement amount of the pixel shift digital camera at low cost and in a short time. An object of the present invention is to provide a microscope pixel shift digital camera system.
[0015]
[Means for Solving the Problems]
In order to solve the above-described problem, an adjustment method for a pixel shift digital camera according to the first aspect of the present invention provides an optical image of an adjustment sample having a figure having at least two straight edges to an imaging unit. A first step of disposing at a predetermined initial position, a second step of setting a driving voltage in the pixel shift unit, and changing a relative position between the optical image of the adjustment sample and the imaging unit, and an imaging unit. A third step of capturing an optical image of the adjustment specimen, a fourth step of obtaining a horizontal component value of the displacement from the initial position for a point on the first edge of the captured figure, For the points on the second edge of the imaged figure, the fifth step for obtaining the vertical component value of the displacement from the initial position and the second to fifth steps are the optical image of the adjustment sample. Drive voltage to vary the relative position of the image sensor and the image pickup means. The optical image of the adjustment sample and the imaging means are obtained from the sixth step which is set and repeated in the means, the plurality of drive voltages set in the image shift means and the horizontal and vertical component values of the obtained displacement amounts. And a seventh step of obtaining a drive voltage set in the image shift means so that the relative position becomes a predetermined value.
[0016]
A pixel shift digital camera system according to another aspect of the present invention drives an image pickup means for picking up an incident light image, a pixel shift means for changing the relative position between the incident light image and the image pickup means, and the pixel shift means. Drive voltage storage means for storing a plurality of drive voltages for driving, pixel shift drive means for driving the pixel shift means based on the drive voltage stored in the drive voltage storage means, and at least two linear edges An adjustment sample having a figure, an imaging control unit that causes the imaging unit to capture an optical image of the adjustment sample for each position at which the pixel shift unit is driven corresponding to the stored drive voltage, and a relative position of the captured image Based on two different images, horizontal relative displacement amount calculating means for obtaining a horizontal direction component value of the displacement amount at a point on the first edge of the figure, and two different relative positions of the captured images The vertical relative displacement amount calculation means for obtaining the vertical component value of the displacement amount for the point on the second end side of the figure based on the image of the image, the drive voltage applied to the image shift means and the obtained displacement amount Drive voltage calculation means for obtaining a drive voltage at which the relative position between the adjustment sample and the imaging means becomes a predetermined value from the horizontal and vertical direction component values, and storage update means for storing the calculated drive voltage in the drive voltage storage means. I have.
[0017]
An adjustment sample according to another aspect of the present invention is an adjustment sample used for a pixel shift digital camera system, and has a figure having at least two straight edges.
[0018]
In addition, a microscope pixel shift digital camera system according to another aspect of the present invention captures an optical image of a specimen by the microscope having a stage on which the specimen is loaded and an objective lens arranged opposite to the specimen, and the objective lens. The image pickup means, the pixel shift means for changing the relative position of the sample optical image and the image pickup means, the drive voltage storage means for storing the drive voltage for driving the pixel shift means, and the drive voltage storage means The pixel shift driving means for driving the pixel shift means based on the drive voltage, the adjustment sample having a figure having at least two linear edges, and the pixel shift means are driven in accordance with the stored drive voltage. On the first edge of the figure based on the image pickup control means for picking up the optical image of the adjustment sample for each position by the image pickup means and the two images taken at different relative positions Based on the horizontal relative displacement amount calculating means for obtaining the horizontal direction component value of the displacement amount for a certain point and the two images taken with different relative positions, the displacement amount for the point on the second edge of the figure The relative position between the adjustment sample and the imaging means is determined from the vertical relative displacement amount calculating means for obtaining the vertical component value of the image, the drive voltage applied to the image shift means and the horizontal and vertical component values of the obtained displacement amount. Drive voltage calculation means for obtaining a drive voltage to be a value, and storage update means for storing the calculated drive voltage in the drive voltage storage means.
[0019]
An adjustment sample according to another aspect of the present invention is an adjustment sample used for a microscope pixel shift digital camera system, and has a figure having at least two straight edges.
[0020]
DETAILED DESCRIPTION OF THE INVENTION
An adjustment method of the pixel shift digital camera according to the first embodiment will be described.
[0021]
FIG. 1 is a diagram illustrating the principle of pixel shift. A Bayer array CCD shown in (1) of FIG. 1 is pixel-shifted nine times at a 2/3 pixel pitch, thereby obtaining a high-resolution composite image as shown in (2) of FIG. it can. FIG. 2 is a diagram illustrating pixel shift positions. The pixel shift mechanism is required to accurately change the relative position of the image and the image sensor from the position Pos0 to Pos8 in FIG.
[0022]
Next, a method of adjusting the set voltage for the pixel shift mechanism to accurately displace the pixel to the position “◯” in FIG.
[0023]
FIG. 3 is a flowchart showing the adjustment method of the present embodiment. A method of adjusting the drive voltage to the position of Pos4 in FIG. 2 will be described with reference to this flowchart and FIG.
[0024]
In step S101, as shown in FIG. 4, the adjustment specimen is installed with an inclination of about 5 degrees with respect to the horizontal and vertical directions of the image sensor. In this state, photographing is performed at each position where the pixel shift mechanism is displaced to Pos1, Pos3, Pos4a, Pos4b, Pos4c, and Pos4d. Here, Pos4a, Pos4b, Pos4c, and Pos4d are points indicating the horizontal and vertical positions around Pos4, and are represented by crosses in FIG.
[0025]
In step S102, the horizontal position difference between the position of the edge part a of Pos3 and the position of the edge part a of Pos4a and Pos4b from the images photographed by Pos4a and Pos4b, which are positions in the vicinity of Pos3 and Pos4 in the horizontal direction, is obtained. Ask. Here, the edge part a represents one end (edge) of the adjustment specimen tilted as shown in FIG.
[0026]
FIG. 6 is a diagram focusing on the edge portion a. When the luminance signal level of the image along the straight line c parallel to the x-axis is graphed, it is as shown in FIG. The three curves in FIG. 7 indicate luminance signal curves of Pos3, Pos4a, and Pos4b, respectively. The half-value position of each curve shown by the dotted line in FIG. 7 is obtained, and the displacement amount between the half-value position of Pos3 and the half-value positions of Pos4a and Pos4b is obtained.
[0027]
When the luminance signal is Y = 0.3R + 0.59G + 0.11B and the relative displacement of Pos4a with respect to Pos3 is expressed by changing the position in the y direction in FIG. 6, the result is as shown in FIG. Here, the y-axis in FIG. 8 represents the amount of displacement (number of pixel pitches) from Pos3 to Pos4a, and the x-axis represents the number of pixels in the vertical direction. As shown in FIG. 8, the obtained displacement amount changes periodically with a target displacement amount 2/3 pixel pitch (≈0.67) as a center.
[0028]
Similarly, when the G signal is used as the luminance signal, the level is small but periodically changes as shown in FIG. In the case of the Y signal, the resolution periodically changes because the resolution of the R and B pixels is lower than that of G in the case of the Bayer array, so that moire occurs, and as a result, the amount of displacement varies depending on the position in the vertical direction. It is to do. The G signal is smaller in size than the Y signal, but a periodic change of 1/2 cycle of the Y signal is observed. The reason why the change period of the G signal is ½ that of the Y signal is that the G pixel is used more frequently than the R and B pixels in the Bayer array, and the appearance frequency is high.
[0029]
Therefore, it can be understood that an accurate displacement amount cannot be measured because an error included in the displacement amount changes depending on which position of the edge is measured. Therefore, the edge portion is inclined with respect to the horizontal direction and the vertical direction so that the position of the edge to be measured is not fixed to a constant value in the horizontal direction and the vertical direction, and a plurality of displacement amounts are set at different positions of the edge. The error can be reduced by measuring and averaging the measured values.
[0030]
FIG. 10 is a diagram showing the relationship between the period of change and the slope of the edge.
[0031]
8 and 9, with the number of pixels shown on the horizontal axis as a unit, the number of pixels in which the change repeats (hereinafter referred to as “period” for convenience) is T pixels, and the inclination of the edge with respect to the horizontal and vertical directions is θ degrees. During one period, the Y signal changes by two pixels in the horizontal direction, and the G signal changes by one pixel.
[0032]
Therefore, the relationship of equation (1) is established for the Y signal, and the relationship of equation (2) is established for the G signal.
[0033]
For Y signal: T = 2 / tan θ (1)
For G signal: T = 1 / tan θ (2)
FIG. 11 is a diagram showing the relationship between the number of cycles averaged for the Y signal and the averaged result, and FIG. 12 is a diagram showing the relationship between the number of cycles averaged for the G signal and the averaged result.
[0034]
11 and 12, it can be seen that measurement can be performed with an error level that is not problematic in practice by averaging the number of pixels of one period or more. If the number of pixels to be averaged in the vertical direction is H, a relationship of H> T needs to be established. Therefore, when this equation is modified to obtain the inclination θ of the edge portion, equation (3) is established for the Y signal and equation (4) is established for the G signal.
For Y signal: θ> tan ^-1 (2 / H) (3)
For G signal: θ> tan ^-1 (1 / H) (4)
As described above, the relative displacement amounts of Pos4a and Pos4b with respect to Pos3 at each y position are averaged to determine the horizontal positions of Pos4a and Pos4b with respect to Pos3.
[0035]
In step S103, the pixel shift mechanism is displaced to the target position based on the relationship between the horizontal driving voltage when the pixel shift mechanism is displaced to positions Pos3, Pos4a, and Pos4b and the horizontal edge position obtained in step S102. Calculate the set voltage.
[0036]
The horizontal driving voltages when displaced to the positions Pos3, Pos4a and Pos4b are Vx3, Vx4a and Vx4b, and the relative displacements of Pos4a and Pos4b obtained in step S102 are S4a and S4b. Assuming that the target displacement of Pos4 is S4x and the driving voltage at that time is Vx4, the driving voltage can be obtained by Expression (5).
[0037]
Vx4 = (Vx4b−Vx4a) / (S4b−S4a) × (S4x−S4a)
+ Vx4a (5)
When a piezoelectric element is used as the pixel shift mechanism, the relationship between the drive voltage and the displacement amount is not linear, but there is no problem even if it is assumed linear because the difference between S4a and S4b is small.
[0038]
In step S104, the vertical positions of the edge portions b of Pos4c and Pos4d are obtained from the images photographed by Pos1, Pos4c, and Pos4d with reference to the position of the edge portion b of Pos1. Then, the relative displacement amount is calculated by the same method as in step S102.
[0039]
In step S105, when the pixel shift mechanism is displaced to the target position based on the relationship between the vertical driving voltage when the pixel shift mechanism is displaced to positions Pos1, Pos4c, and Pos4d and the vertical edge position obtained in step S104. Calculate the set voltage.
[0040]
The vertical driving voltages when displaced to the positions Pos1, Pos4c and Pos4d are Vy1, Vy4c and Vy4d, and the relative displacements of Pos4c and Pos4d obtained in step S104 are S4c and S4d. Assuming that the target displacement of Pos4 is S4y and the driving voltage at that time is Vy4, the driving voltage can be obtained by Expression (6).
[0041]
Vy4 = (Vy4d−Vy4c) / (S4d−S4c) × (S4y−S4c)
+ Vy4c (6)
By performing the above steps for Pos1 to Pos8, the driving voltage at each pixel shift position can be calculated. However, the vertical driving voltage of Pos1 and Pos2 is set to be the same as Pos0, and the horizontal driving voltage of Pos3 and Pos7 is set to be equal to that of Pos0. Usually, since the orthogonality of the horizontal and vertical displacements of the pixel shift mechanism is good, this is not a problem.
[0042]
By adjusting the pixel shift digital camera as described above, the shift displacement amount of the pixel shift digital camera can be accurately adjusted without increasing cost and adjustment time.
[0043]
Next, a first modification of the adjustment method of the first embodiment will be described.
[0044]
In the above-described edge position calculation, the displacement amount of the pixel shift mechanism is obtained from the average displacement amount of the position of each point of the edge, but can also be obtained as follows.
[0045]
The measurement of the amount of horizontal displacement of the edge part a of FIG. 6 is demonstrated.
[0046]
Similar to the above-described embodiment, the x and y coordinates of the half-value position of the edge are obtained for each of Pos3, Pos4a, and Pos4b. When these x and y coordinates are expressed as plane coordinates, three straight lines shown in FIG. 13 are obtained.
[0047]
Next, equations of the respective straight lines are obtained by the method of least squares to obtain Equations (7), (8), and (9).
[0048]
About Pos3: x = p3 * y + q3 (7)
About Pos4a: x = p4a * y + q4a (8)
About Pos4b: x = p4b * y + q4b (9)
Here, p3, p4a, p4b, q3, q4a, and q4b are constants.
[0049]
The relative displacement is calculated with the x position of Pos3, Pos4a and Pos4b when the y-direction position takes the median value in the above equation as the position of the straight line at each pixel shift position. In the example of FIG. 13, the value of x when y = 40 is used.
[0050]
When the relative displacement is obtained in this way, the influence of the above-mentioned periodic change is removed by the least square method, so that the displacement amount can be measured with high accuracy as in the above-described embodiment. Then, the drive voltage is calculated according to the displacement amount in the same manner as described above.
[0051]
Next, a second modification of the adjustment method according to the first embodiment will be described.
[0052]
FIG. 14 is a diagram illustrating the imaging position of the specimen. As shown in FIG. 14, sample images are taken at positions displaced by four positions (× positions) in the vertical and horizontal directions for each pixel shift position from Pos0 to Pos8. Hereinafter, a method for calculating the driving voltage of Pos5 will be described.
[0053]
First, the adjustment specimen is photographed at positions Pos5a, Pos5b, Pos5c, and Pos5d. As described in the first modification, the edge straight line equation is obtained for the edge portions a and b of the images photographed at the respective positions, and the intersection of the two straight line equations is obtained. As a result, (x5a, y5a), (x5b, y5b), (x5c, y5c), and (x5d, y5d) are obtained as intersections of Pos5a, Pos5b, Pos5c, and Pos5d.
[0054]
When driving voltages when the pixel shift mechanism is displaced to Pos5a, Pos5b, Pos5c, Pos5d are (V5xa, V5ya), (V5xb, V5yb), (V5xc, V5yc), (V5xd, V5yd), Pos5 is the target position. The drive voltages (V5x, V5y) for displacing to (x5, y5) are expressed by equations (10) and (11).
[0055]
V5x = (V5xb-V5xa) / (x5b-x5a) * (x5-x5a)
+ V5xb (10)
V5y = (V5yd−V5yc) / (y5d−y5c) × (y5−y5c)
+ V5yc (11)
This process is performed for all of Pos0 to Pos8, and the drive voltage for each target position is calculated.
[0056]
According to the second modification, the vertical positions of Pos1 and Pos2 and the horizontal positions of Pos3 and Pos6 depend on the orthogonality of the pixel shift mechanism in the first embodiment and the first modification. On the other hand, even when the orthogonality of the pixel shift mechanism is poor, the drive voltage can be adjusted with high accuracy.
[0057]
Next, a third modification of the adjustment method according to the first embodiment will be described.
[0058]
In the third modified example, the adjustment method shown in the first embodiment and the first and second modified examples is repeatedly performed to adjust with higher accuracy.
[0059]
After adjusting the target drive voltage based on the processing at the position indicated by x in FIGS. 2 and 14, the target drive voltage is reset to a narrower range as indicated by Δ in FIG. 15 based on the drive voltage. Thus, the driving voltage at the target position is calculated.
[0060]
By performing processing based on data at a position closer to the target position, the displacement measurement error can be reduced.
[0061]
This resetting process may be repeated a predetermined number of times to improve the adjustment accuracy, or may be determined to have converged because the difference from the previously obtained target position drive voltage has become smaller.
[0062]
Next, a second embodiment of the present invention will be described. In the second embodiment, the pixel shift digital camera system of the present invention is applied to the photographing of a specimen image of a microscope, but the microscope is used as a camera lens, and the size of the specimen is changed as appropriate. Application to a pixel shift digital camera system for photographing a subject is also possible.
[0063]
FIG. 16 is a diagram illustrating a configuration of a microscope digital camera system according to the present invention, and FIG. 17 is a diagram illustrating a configuration of a signal processing unit used in the microscope digital camera system according to the present invention.
[0064]
In FIG. 16, the illumination light emitted from the light source 4 of the microscope body 2 illuminates the sample S on the stage 11 via the lens 5, mirror 6, lens 7, field stop 8, aperture stop 9, and condenser lens 10. To do. Here, the stage 11 can be moved and adjusted in the horizontal direction and the vertical direction within a plane perpendicular to the optical axis direction and the optical axis.
[0065]
The image of the sample S by the illumination light is imaged by the objective lens 12 and the imaging lens 13 and enters the prism 14. In the prism 14, the image of the sample S is branched, one is emitted through the eyepiece 15, and the other is made incident through the camera adapter 30 into the camera head unit 1 of the microscope digital camera.
[0066]
In the camera head unit 1, an incident image is formed on an image sensor 16 such as a CCD, and image processing or the like is performed by a control processing unit 18. The camera head unit 1 is connected to the PC 3 via the cable 19.
[0067]
Next, the camera head unit will be described with reference to FIG.
[0068]
In the camera head unit 1, an image sensor 16 such as a CCD is attached to a pixel shift mechanism 17 including an xy stage that can be moved in the xy direction by a piezoelectric element. The CCD is, for example, a Bayer array single-plate color CCD, and its pixel pitch is 6 μm.
[0069]
A control processing unit 18 is connected to the image sensor 16. The image sensor 16 photoelectrically converts the optical image of the specimen imaged on the image sensor 16 by a drive signal from the image sensor drive unit 20 and outputs a signal corresponding thereto to the pre-processing unit 19.
[0070]
An imaging element driving unit 20 and an amplifying unit 21 are connected to the pre-processing unit 19. The pre-processing unit 19 synchronizes with the imaging device 16 by the synchronization signal from the imaging device driving unit 20 and outputs the input signal to the amplification unit 21 as a video signal.
[0071]
A preprocessing unit 19, an A / D conversion unit 22, and a control unit 28 are connected to the amplification unit 21. The amplification unit 21 amplifies the video signal from the pre-processing unit 19 according to the gain setting by the control unit 28 and outputs the amplified video signal to the A / D conversion unit 22.
[0072]
The A / D conversion unit 22 is connected to the image sensor driving unit 20, the amplification unit 21, and the memory controller 23. In the A / D conversion unit 22, the video signal from the amplification unit 21 is digitized in synchronization with the imaging device 16 by the synchronization signal from the imaging device driving unit 20, and is output to the memory controller 23.
[0073]
Connected to the memory controller 23 are an A / D converter 22, a memory 24, a signal processor 25 and a bus 26. The memory controller 23 outputs a digital signal from the A / D conversion unit 22 to the memory 24 according to control from the control unit 28 via the bus 26, and outputs the digital signal to the signal processing unit 25.
[0074]
The signal processing unit 25 is connected to the bus 26, and performs predetermined signal processing such as pixel interpolation from the Bayer array and color correction under the control of the control unit 28 via the bus 26, and the processing result digital signal is transmitted to the bus 26. Is output to the control unit.
[0075]
An I / F 29 is connected to the control unit 28, and a PC 3 is further connected via the I / F 29. The control unit 28 outputs the input image data to the PC 3 via the I / F 29.
[0076]
A pixel shift mechanism drive unit 31 is connected to the pixel shift mechanism 17, and a control unit 28 is further connected. The control unit 28 reads out a voltage value for moving the pixel shift mechanism 17 stored in the pixel shift mechanism drive voltage storage unit 27 connected to the bus 26 to a predetermined position when taking a still image to be described later. The pixel shift mechanism drive unit 31 is set to displace the pixel shift mechanism 17.
[0077]
An operation screen as shown in FIG. 18 is displayed on the monitor of the PC 3, and the optical image of the specimen is sent to the control unit 28 via the I / F 29 based on an operation instruction by a pointing device such as a mouse on a screen (not shown). Instructs still image acquisition, electronic shutter setting of the image sensor drive unit 20, gain setting of the amplification unit 21, and the like.
[0078]
Usually, the camera head operates in a moving image mode, and the above-described processing is performed at a dozen frames per second, and an image of a specimen observed with a microscope is displayed on the operation screen as a live image.
[0079]
Next, a pixel shift operation and a still image shooting operation will be described.
[0080]
When a still image shooting button is pressed on the operation screen and a shooting instruction is issued from the PC 3 to the control unit 28 accordingly, still image shooting is performed in the following order. In this embodiment, a pixel shift method using a 2/3 pixel pitch and 9 shifts will be described as an example. In this case, the pixel shift mechanism 17 moves the image sensor 16 to (0, 0), (2/3 pixel, 0), (4/3 pixel, 0), (0, 2/3 pixel), (2/3 pixel). , 2/3 pixel), (4/3 pixel, 2/3 pixel), (0, 4/3 pixel), (2/3 pixel, 4/3 pixel), (4/3 pixel, 4/3 pixel) ) To 9 positions.
[0081]
Procedure 1: The control unit 28 reads the drive voltage at the initial position from the pixel shift mechanism drive voltage storage unit 27 and sets it in the pixel shift mechanism drive unit 31.
[0082]
Procedure 2: The pixel shift mechanism drive unit 31 displaces the pixel shift mechanism 17 according to the set voltage.
[0083]
Procedure 3: The control unit 28 instructs the image sensor driving unit 20 to read out charges from the image sensor 16, and instructs the memory controller 23 to store the captured image data in the memory 24.
[0084]
Procedure 4: The control unit 28 reads the driving voltage at the second position from the pixel shift mechanism driving voltage storage unit 27 and sets it in the pixel shift driving unit 31.
[0085]
Procedure 5: The pixel shift mechanism drive unit 31 displaces the pixel shift mechanism 17 according to the set voltage.
[0086]
Procedure 6: The control unit 28 instructs the image sensor driving unit 20 to read out charges from the image sensor 16, and instructs the memory controller 23 to store the captured image data in the memory 24.
[0087]
Step 7: Repeat Step 4 to Step 6 for the third to ninth positions.
[0088]
Procedure 8: After the pixel shift photographing is completed, the control unit 28 instructs the memory controller to read from the memory 23.
[0089]
Step 9: The memory controller 23 reads out the image data for a plurality of sheets stored in the memory 24 by the above-described pixel shift photographing so that the relative positional relationship is adjusted in consideration of the pixel shift as shown in FIG. To the unit 25.
[0090]
Procedure 10: The signal processing unit 25 processes the input signal in response to an instruction from the control unit 28 and outputs the processed signal to the control unit 28.
[0091]
Procedure 11: The control part 28 transfers the image data of a process result to PC3.
[0092]
Procedure 12: The PC 3 stores the transferred image data in the memory in the PC 3, displays it on the monitor, and completes the photographing.
[0093]
Procedure 13: The image data stored in the memory of the PC 3 is appropriately saved in the hard disk.
[0094]
The microscope pixel shift digital camera system of the second embodiment has the following configuration and functions in addition to the configuration of the above-described microscope digital camera.
[0095]
(1) Specimen for adjustment
In the adjustment specimen shown in FIG. 20, a figure is formed on the slide glass 40 by chromium vapor deposition or the like. The figure is composed of an outer frame 41 parallel to the horizontal / vertical direction of the image sensor and a square 42 inclined by 5 degrees with respect to the horizontal / vertical direction of the image sensor.
[0096]
(2) A function of storing pixel shift mechanism drive voltage data for giving displacement of the pixel shift mechanism 17 indicated by ◯ Δ in FIG. 21 by a program on the PC 3.
[0097]
This data may be stored in the pixel shift mechanism drive voltage storage unit 27.
[0098]
In FIG. 21, the position where the pixel shift mechanism should be displaced during still image shooting is indicated by ◯, and the interval is 2/3 of the pixel pitch of the image sensor. Pos0 is the initial position, and imaging is performed at nine positions up to Pos8, and the images are synthesized to obtain a high-definition still image. In the initial state, the position of .DELTA..DELTA. Does not need to be an accurate position, and may be a position that is expected to have a position relationship of .DELTA..DELTA. In an unadjusted pixel shift mechanism.
[0099]
(3) As a function by a program on the PC, pixel shift mechanism drive voltage data is set to the pixel shift mechanism drive unit 31 via the control unit 28 of the camera head 1, and a sample image is taken in that state, and a predetermined image is obtained. Function to perform signal processing and import to PC.
[0100]
(4) A function for calculating the horizontal position of the edge portion a of the sample in FIG.
[0101]
(5) A function for calculating the vertical position of the edge portion b of the sample in FIG.
[0102]
(6) As a function by the program on the PC, the relationship between the input voltage to the pixel shift mechanism and the displacement amount is obtained from the relationship between the plurality of sets of pixel shift mechanism drive voltage data and the position of the edge portion, and the input to the target movement amount Function to calculate voltage.
[0103]
Next, an adjustment operation for displacing the pixel shift mechanism 17 to a target position in the microscope pixel shift digital camera system according to the present embodiment will be described with reference to the flowchart of FIG.
[0104]
In step S201, the adjustment specimen shown in FIG.
[0105]
In step S202, the focus of the sample and the direction of the sample S and the image sensor 16 are adjusted. Focus adjustment is performed by moving the microscope stage up and down. The rotation direction is adjusted by directly rotating the mounting direction of the specimen S, by rotating the adapter 30 to which the camera head 1 is attached, or when the stage 11 has a rotation mechanism, as shown in FIG. Then, the horizontal and vertical directions of the operation screen are adjusted so that the horizontal and vertical directions of the outer frame of the sample coincide with each other.
[0106]
In step S203, the shooting mode is changed to “adjustment” on the operation screen as shown in FIG. 23, and the still image shooting button is pressed.
[0107]
In step S204, the PC 3 takes a sample image when the pixel shift mechanism is at the Pos0 position in FIG. 21 via the control unit 28, and calculates the horizontal position of the edge part a and the vertical position of the edge part b.
In step S205, the PC 3 captures a sample image when the pixel shift mechanism is in the Δ position in FIG.
In step S206, the PC 3 captures a sample image when the pixel shift mechanism 17 is at the ▽ position in FIG. 21 via the control unit 28, and calculates the vertical position of the edge portion b at each position.
[0108]
In step S207, the pixel shift mechanism drive voltage at the correct target position ◯ shown in FIG. 21 is calculated by the calculation method described in the first embodiment.
[0109]
In step S208, the calculated drive voltage value is stored in the pixel shift mechanism drive voltage storage unit 27 via the control unit 28.
[0110]
According to the configuration and operation described above, it is possible to provide a pixel shift digital camera system capable of accurately adjusting the pixel shift displacement amount of the pixel shift digital camera without increasing cost and adjustment time.
[0111]
As a modification of the sample, the shape of the adjustment sample is not limited to that shown in FIG. 20 as long as the sample has an edge portion that is inclined with respect to the horizontal direction and the vertical direction. Can be considered. For example, it may be as shown in FIG.
[0112]
If an edge portion is formed on a part of a slide glass that is usually used for a microscope, an adjustment sample is always attached to a sample for daily use, and adjustment can be made at any time. Furthermore, as shown in FIG. 25, it is also possible to form an edge on a slide glass with a bar code in order to arrange the specimen numbers.
[0113]
Furthermore, as shown in FIG. 26 and FIG. 27, an adjustment specimen is formed with a test chart, letters, numbers, and a geometric pattern together with the edge portion so that the pattern formed with the edge portion before and after the adjustment can be displayed. good. By confirming that the shape of the comparative image is displayed smoothly, the operator can confirm the effect of the adjustment.
[0114]
Further, as a modification of the operation screen in the case of dedicated adjustment at the time of manufacture, as shown in FIG. 28, a still image shooting button or the like may not be provided, but only a button for starting adjustment may be provided, and a frame is provided on the specimen. Instead, a straight line representing the inclination of the edge to be matched may be displayed on the operation screen.
[0115]
As a modification of the operation screen of the commercial application, as shown in FIG. 29, the pixel shift mechanism adjustment start may be put under the menu option and displayed in a pull-down menu. In the above-described embodiment, the adjustment mode is directly changed on the operation screen. However, since two actions are required for the adjustment start operation by placing it under the option and displaying it in the pull-down menu, an erroneous operation can be prevented. .
[0116]
Further, as a modification of the microscope configuration, the adjustment specimen may be arranged in the microscope so that it can be inserted and removed at the position of the field stop 8 in FIG. This is because the position of the field stop 8 is an optically conjugate position with the sample surface, and an optical image at this position is also formed on the image sensor surface.
[0117]
By incorporating the adjustment specimen into the microscope in this way, there are effects such as prevention of loss of the adjustment specimen.
[0118]
In addition, since the field stop 8 is reduced as shown in FIG. 30, the field stop 8 itself may be used as an adjustment sample. As a result, it is not necessary to attach an adjustment specimen, and the cost can be reduced.
[0119]
Some adapters have a relay lens optical system with an imaging position in the middle, such as an adapter 30a shown in FIG. In such a case, an adjustment specimen may be placed at the intermediate imaging position 32. By incorporating the adjustment specimen into the microscope in this way, there are effects such as prevention of loss of the adjustment specimen.
[0120]
In addition, this invention can be comprised as follows based on each embodiment described above.
[0121]
(1) a first step of disposing an optical image of an adjustment specimen having a figure having at least two straight edges on a predetermined initial position with respect to the imaging means;
A second step of setting a driving voltage in the pixel shift means and changing a relative position between the optical image of the adjustment sample and the imaging means;
A third step of capturing an optical image of the adjustment specimen by the imaging means;
For each of a plurality of points on the first edge of the imaged figure, the horizontal component value of the displacement amount from the initial position is obtained, and the average horizontal component value obtained by averaging the plurality of horizontal component values A fourth step of calculating
For each of a plurality of points on the second edge of the imaged figure, the vertical component value of the displacement amount from the initial position is obtained, and the average vertical component value obtained by averaging the plurality of vertical component values A fifth step of calculating
A sixth step in which the second to fifth steps are repeated by setting a driving voltage in the pixel shift unit to change the relative positions of the optical image of the adjustment sample and the imaging unit, and
Based on a plurality of drive voltages set in the image shift means and the obtained average horizontal direction component value and average vertical direction component value, the relative position between the optical image of the adjustment sample and the imaging means becomes a predetermined value. A seventh step of obtaining a drive voltage set in the image shift means;
A method for adjusting a pixel shift digital camera, comprising:
[0122]
(2) a first step of arranging an optical image of an adjustment specimen having a figure having at least two straight edges at a predetermined initial position with respect to the imaging means;
A second step of setting a driving voltage in the pixel shift means and changing a relative position between the optical image of the adjustment sample and the imaging means;
A third step of capturing an optical image of the adjustment specimen by the imaging means;
In a coordinate system set in the captured image, a straight line expression representing the first edge of the captured graphic is obtained by the least square method, and the displacement from the initial position is calculated based on the straight line expression. A fourth step of obtaining a horizontal component value of
In a coordinate system set in the captured image, a straight line expression representing the second edge of the captured graphic is obtained by the least square method, and the displacement amount from the initial position based on the straight line expression A fifth step of obtaining a vertical direction component value of
A sixth step in which the second to fifth steps are repeated by setting a driving voltage in the pixel shift unit to change the relative positions of the optical image of the adjustment sample and the imaging unit, and
Based on a plurality of drive voltages set in the image shift means and horizontal and vertical component values of the obtained displacement amounts, the relative position between the optical image of the adjustment sample and the imaging means becomes a predetermined value. A seventh step of obtaining a drive voltage set in the image shift means;
A method for adjusting a pixel shift digital camera, comprising:
[0123]
(3) a first step of disposing an optical image of an adjustment specimen having a figure having at least two straight edges on a predetermined initial position with respect to the imaging means;
A second step of setting a driving voltage in the pixel shift means and changing a relative position between the optical image of the adjustment sample and the imaging means;
A third step of capturing an optical image of the adjustment specimen by the imaging means;
A fourth step of obtaining, in a coordinate system set in the imaged image, a straight line expression representing the first edge of the imaged figure by the least square method;
A fifth step of obtaining, in a coordinate system set in the captured image, an equation of a straight line representing a second edge of the captured graphic by a least square method;
The sixth step for obtaining the coordinates of the intersection point based on the obtained two straight line formulas and the second to sixth steps are different from each other in that the relative positions of the optical image of the adjustment sample and the imaging means are different. A seventh step of setting and repeating the driving voltage to be set in the pixel shift means;
From the plurality of drive voltages set in the image shift means and the obtained coordinate values of the plurality of intersections, the image shift means is set so that the relative position between the optical image of the adjustment sample and the imaging means becomes a predetermined value. An eighth step for obtaining a driving voltage to be set;
A method for adjusting a pixel shift digital camera, comprising:
[0124]
(4) In the adjustment method of the pixel shift digital camera according to (1) to (3) above,
The imaging means is a single-plate color imaging device;
The method of adjusting a pixel shift digital camera, wherein the captured figure is represented by a luminance signal obtained from an RGB signal which is a video signal from the single-plate color image sensor.
[0125]
(5) In the adjustment method of the pixel shift digital camera according to (1) to (3) above,
The imaging means is a single-plate color imaging device;
The method of adjusting a pixel shift digital camera, wherein the captured figure is represented by a G signal of RGB signals which are video signals from the single-plate color image sensor.
[0126]
(6) a first step of disposing an optical image of an adjustment specimen having a figure having at least two straight edges on a predetermined initial position with respect to the imaging unit;
A second step of setting a driving voltage in the pixel shift means and changing a relative position between the optical image of the adjustment sample and the imaging means;
A third step of capturing an optical image of the adjustment specimen by the imaging means;
A fourth step of obtaining a horizontal component value of a displacement amount from an initial position for a point on the first edge of the imaged figure;
A fifth step of obtaining a vertical component value of a displacement amount from an initial position for a point on the second edge of the imaged figure;
A sixth step in which the second to fifth steps are repeated by setting a driving voltage in the pixel shift unit to change the relative positions of the optical image of the adjustment sample and the imaging unit, and
Based on a plurality of drive voltages set in the image shift means and horizontal and vertical component values of the obtained displacement amounts, the relative position between the optical image of the adjustment sample and the imaging means becomes a predetermined value. A seventh step of obtaining a drive voltage set in the image shift means;
An eighth step in which the driving voltage of the second step is updated to a driving voltage having a smaller difference from the driving voltage obtained in the seventh step, and the second to seventh steps are repeated;
A method for adjusting a pixel shift digital camera, comprising:
[0127]
(7) a microscope having a stage on which a specimen is loaded and an objective lens arranged opposite to the specimen;
An image pickup means for picking up an optical image of the sample by the objective lens;
Pixel shift means for changing a relative position between the optical image of the sample and the imaging means; drive voltage storage means for storing a drive voltage for driving the pixel shift means;
Pixel shift drive means for driving the pixel shift means based on the drive voltage stored in the drive voltage storage means;
An adjustment sample having a figure with at least two straight edges;
An imaging control unit that causes the imaging unit to capture an optical image of the adjustment sample for each position at which the pixel shift unit is driven corresponding to the stored drive voltage;
A horizontal relative displacement amount calculating means for obtaining a horizontal direction component value of a displacement amount for a point on the first edge of the graphic, based on the two images taken with different relative positions;
A vertical relative displacement amount calculating means for obtaining a vertical direction component value of a displacement amount for a point on the second end side of the figure based on the two images taken with different relative positions;
Drive voltage calculation means for obtaining the drive voltage at which the relative position between the adjustment sample and the imaging means is a predetermined value from the drive voltage applied to the image shift means and the horizontal and vertical component values of the obtained displacement. When
Storage update means for storing the calculated drive voltage in the drive voltage storage means;
A microscope pixel shift digital camera system characterized by comprising:
[0128]
(8) In the microscope pixel shift digital camera system described in (7) above,
2. A microscope pixel shift digital camera system characterized in that the adjustment specimen is arranged at a position optically conjugate with an imaging means, which is different from a specimen position on the stage.
[0129]
(9) In the microscope pixel shift digital camera system described in (8) above,
The microscope includes an illumination optical system including an illumination light source, a field stop, and a condenser lens, the adjustment specimen is the field stop, and the figure having the at least two linear edges is the field stop. Microscope pixel shift digital camera system characterized by
[0130]
(10) In the microscope pixel shift digital camera system described in (9) above,
A relay lens optical system between the objective lens and the imaging means of the microscope;
A microscope pixel shift digital camera system, wherein the adjustment specimen is detachably disposed at an intermediate image forming position of the relay lens optical system.
[0131]
(11) In the microscope pixel shift digital camera system described in (7) above,
2. A microscope pixel shift digital camera system characterized by comparing and displaying pixel shift still image photographing results of the adjustment specimen before and after storing the calculated drive voltage in the drive voltage storage means.
[0132]
(12) An adjustment specimen having a figure having at least two straight edges used for a pixel shift digital camera system or a microscope pixel shift digital camera system,
A specimen for adjustment characterized by comprising a slide glass.
[0133]
(13) An adjustment specimen having a figure having at least two straight edges used for a pixel shift digital camera system or a microscope pixel shift digital camera system,
An adjustment specimen having a reference line indicating a horizontal direction and a vertical direction of an imaging means.
[0134]
(14) An adjustment specimen having a figure having at least two straight edges used for a pixel shift digital camera system or a microscope pixel shift digital camera system,
A specimen for adjustment characterized by having a barcode.
[0135]
(15) An adjustment specimen having a figure having at least two straight edges used for a pixel shift digital camera system or a microscope pixel shift digital camera system,
An adjustment specimen having at least one of letters, numbers, and geometric patterns.
[0136]
Each embodiment according to the present invention described above has the following effects.
[0137]
According to the above (1), (2), (4), (5), and (6), the shift displacement amount of the pixel shift digital camera can be accurately adjusted without increasing the cost and the adjustment time.
[0138]
According to the above (3), in addition to the above-described effect, it is possible to adjust with high accuracy even when the orthogonality of the pixel shift mechanism is bad.
[0139]
According to the above (6), in addition to the above-described effect, it can be adjusted with higher accuracy.
[0140]
According to the above (7), the shift displacement amount of the pixel shift digital camera can be accurately adjusted without increasing the cost and the adjustment time, and the image quality of the captured image can be improved.
[0141]
According to (12) above, the adjustment specimen can be placed on the stage of the microscope, and the adjustment becomes simple.
[0142]
According to the above (13), the adjustment is simplified by aligning the reference line indicating the horizontal and vertical direction of the imaging means for the specimen for adjustment with the horizontal and vertical direction of the imaging means.
[0143]
According to the above (8) and (10), it can be incorporated into a microscope, and convenience is improved.
[0144]
According to the above (11), a part of a conventional microscope configuration can be used as a specimen, and the cost can be reduced.
[0145]
According to the above (11), (14), and (15), the adjustment accuracy can be easily confirmed, and the adjustment reliability can be improved.
[0146]
【The invention's effect】
As described above, according to the present invention, the shift displacement amount of the pixel shift digital camera can be accurately adjusted in a low cost and in a short time.
[Brief description of the drawings]
FIG. 1 is a diagram showing the principle of pixel shift.
FIG. 2 is a diagram showing a shift position of a pixel.
FIG. 3 is a flowchart showing an adjustment method.
FIG. 4 is a diagram illustrating an installation example of an adjustment specimen.
FIG. 5 is a diagram showing an edge portion.
FIG. 6 is a view showing an edge portion.
FIG. 7 is a diagram showing luminance signal levels.
FIG. 8 is a diagram showing a relative displacement in the vertical direction.
FIG. 9 is a diagram showing a relative displacement in the vertical direction.
FIG. 10 is a diagram showing a relationship between a change cycle and an edge inclination;
FIG. 11 is a diagram showing the relationship between the number of periods to be averaged for the Y signal and the averaged result.
FIG. 12 is a diagram showing the relationship between the number of periods averaged for a G signal and the averaged result.
FIG. 13 is a diagram showing three straight lines.
FIG. 14 is a diagram showing a photographing position of a specimen.
FIG. 15 is a diagram showing a photographing position of a specimen.
FIG. 16 is a diagram showing a configuration of a microscope digital camera system.
FIG. 17 is a diagram showing a configuration of a signal processing unit used in a microscope digital camera system.
FIG. 18 is a diagram showing an operation screen.
FIG. 19 is a diagram showing image composition.
FIG. 20 is a view showing a specimen for adjustment.
FIG. 21 is a diagram showing a photographing position of a specimen.
FIG. 22 is a flowchart showing an adjustment operation for displacing the pixel shift mechanism to a target position.
FIG. 23 is a diagram showing an operation screen.
FIG. 24 is a diagram showing a specimen for adjustment.
FIG. 25 is a diagram showing a specimen for adjustment.
FIG. 26 is a view showing a specimen for adjustment.
FIG. 27 is a view showing a specimen for adjustment.
FIG. 28 is a diagram showing an operation screen.
FIG. 29 is a diagram showing an operation screen.
FIG. 30 shows a field stop.
FIG. 31 is a diagram showing the installation position of a specimen for adjustment.
FIG. 32 is a diagram illustrating a configuration of a digital camera system in which a digital camera using a pixel shift method is applied to capturing a specimen image of a microscope.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 ... Camera head part, 2 ... Microscope main body, 3 ... PC, 8 ... Field stop,
11 ... Stage, 12 ... Objective lens, 16 ... Image sensor,
17 ... Pixel shift mechanism, 25 ... Signal processing unit, 27 ... Pixel shift mechanism drive voltage storage unit, 30 ... Adapter, 31 ... Pixel shift mechanism drive unit,
32 ... Intermediate imaging position, 40 ... Slide glass

Claims (5)

A first step of disposing an optical image of an adjustment specimen having a figure having at least two straight edges at a predetermined initial position with respect to the imaging means;
A second step of setting a driving voltage in the pixel shift means and changing a relative position between the optical image of the adjustment sample and the imaging means;
A third step of capturing an optical image of the adjustment specimen by the imaging means;
A fourth step of obtaining a horizontal component value of a displacement amount from an initial position for a point on the first edge of the imaged figure;
A fifth step of obtaining a vertical component value of a displacement amount from an initial position for a point on the second edge of the imaged figure;
A sixth step in which the second to fifth steps are repeated by setting a driving voltage in the pixel shift unit to change the relative positions of the optical image of the adjustment sample and the imaging unit, and
Based on a plurality of drive voltages set in the image shift means and horizontal and vertical component values of the obtained displacement amounts, the relative position between the optical image of the adjustment sample and the imaging means becomes a predetermined value. And a seventh step of obtaining a driving voltage to be set in the image shift means. An imaging means for capturing an incident light image;
Pixel shift means for changing a relative position between the incident light image and the imaging means;
Drive voltage storage means for storing a plurality of drive voltages for driving the pixel shift means;
Pixel shift drive means for driving the pixel shift means based on the drive voltage stored in the drive voltage storage means;
An adjustment sample having a figure with at least two straight edges;
An imaging control unit that causes the imaging unit to capture an optical image of the adjustment sample for each position at which the pixel shift unit is driven corresponding to the stored drive voltage;
A horizontal relative displacement amount calculating means for obtaining a horizontal direction component value of a displacement amount for a point on the first edge of the graphic, based on the two images taken with different relative positions;
A vertical relative displacement amount calculating means for obtaining a vertical direction component value of a displacement amount for a point on the second end side of the figure based on the two images taken with different relative positions;
Drive voltage calculation means for obtaining the drive voltage at which the relative position between the adjustment sample and the imaging means is a predetermined value from the drive voltage applied to the image shift means and the horizontal and vertical component values of the obtained displacement. And a storage update means for storing the calculated drive voltage in the drive voltage storage means. An adjustment sample for use in a pixel shift digital camera system, the adjustment sample having a figure having at least two straight edges. A microscope having a stage on which a specimen is loaded and an objective lens arranged opposite to the specimen;
An image pickup means for picking up an optical image of the sample by the objective lens;
Pixel shift means for changing a relative position between the optical image of the sample and the imaging means;
Drive voltage storage means for storing a drive voltage for driving the pixel shift means;
Pixel shift drive means for driving the pixel shift means based on the drive voltage stored in the drive voltage storage means;
An adjustment sample having a figure with at least two straight edges;
An imaging control unit that causes the imaging unit to capture an optical image of the adjustment sample for each position at which the pixel shift unit is driven corresponding to the stored drive voltage;
A horizontal relative displacement amount calculating means for obtaining a horizontal direction component value of a displacement amount for a point on the first edge of the graphic, based on the two images taken with different relative positions;
A vertical relative displacement amount calculating means for obtaining a vertical direction component value of a displacement amount for a point on the second end side of the figure based on the two images taken with different relative positions;
Drive voltage calculation means for obtaining the drive voltage at which the relative position between the adjustment sample and the imaging means is a predetermined value from the drive voltage applied to the image shift means and the horizontal and vertical component values of the obtained displacement. And a storage update means for storing the calculated drive voltage in the drive voltage storage means. An adjustment sample used in a microscope pixel shift digital camera system, the adjustment sample having a figure having at least two straight edges.

Images