JP2004144527A - Tissue specimen collecting apparatus, tissue preservation container integral identification tag, and authentication tag mounting method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a tissue specimen collecting apparatus which collects an individual preparation simultaneously and preserves it in a preservation container, a tissue preservation container integral identification tag, and an authentication tag mounting method. <P>SOLUTION: This apparatus has a constitution wherein center parts of two handles 1a, 1b are mutually connected, and, by gripping one end side, the other end side can be moved. The constitution is equipped with a penetration body 2 for penetrating a partial tissue of a body of an animal or a plant on a first tip of the other end, and a tissue collection container part 3 for accepting the penetration body on a second tip of the other end, and storing a container for storing a tissue acquired by cutting the partial tissue of the body of the animal or the plant by a penetration part. By this constitution, the cut tissue is stored in the container connected to the identification tag on which an identification code for identifying the animal or the plant is described simultaneously with identification tag mounting. Hereby, mounting of the identification tag and collection of the preparation is performed simultaneously. <P>COPYRIGHT: (C)2004,JPO

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a tissue sample collecting instrument, a tissue storage container integrated identification tag, and a unique identification tag mounting method. In particular, the present invention provides an identification tag attached with a unique number / symbol for identification of animals and plants, irrespective of livestock animals such as cows, pigs, chickens, horses and crops, vegetables, flowers, trees, etc. The present invention relates to a tissue sample collection instrument, a tissue storage container integrated identification tag and a unique identification tag mounting method that enable simultaneous collection of a tissue sample for research and examination.
[0002]
[Prior art]
Conventionally, as a method of mounting a tissue sample collection instrument, a tissue storage container integrated identification tag, and a unique identification tag, the mounting of an individual identification tag, which has been generally performed so far, is intended only to attach the identification tag to an individual. I have. In addition, collection of tissue specimens for research and inspection purposes is intended only for collection.
[0003]
When collecting tissue and gene specimens of individuals, a method of collecting hair with hair roots from the animal body with an adhesive tape, a method of collecting blood, and in the case of cows, earmarks for individual identification Methods such as collecting ears at a slaughterhouse have been used.
[0004]
On the other hand, in the event of an epidemic such as mad cow disease, from the viewpoint of ensuring the safety of consumers, livestock and crops that are polluted and related should be promptly separated from the market. There is an urgent need to create a tracking system from the origin to the origin.
Based on this necessity, all cattle have already been managed by the Japanese government with a unique number (ear tag number) by law, and each cow has an ear tag (tag with control number) for identification. ) Is required.
[0005]
In the collection of tissue specimens, discrimination testing of animals and plants by genetic analysis has been put to practical use in order to scientifically verify that the above management system is functioning smoothly and properly. This individual identification technology identifies the identity of an individual gene by comparing the gene sequence of a previously stored tissue or gene sample with a tissue or gene sample collected in the market or in the field.
[0006]
Therefore, appropriately collecting and preserving a tissue or a gene sample serving as a basis for performing collation is the basis for such individual identification research / test.
Concerns about the identity and safety of foods of consumers due to the occurrence of mad cow disease in Japan and mislabeling of foods in foods are rapidly increasing.
[0007]
In other words, the collection of individual identification sheets and tissues is treated as separate entities, and sufficient measures are taken against the collection of tissues, including contamination of tissues, hair, saliva, etc. from other individuals. Did not. Similarly, for a plant body, a method is used in which a plant body such as a leaf and a stem is cut off and collected, separately from mounting the identification tag.
[0008]
In managing the legitimacy of food labeling and proper labeling of the place of origin, rationally manage the raw materials in the food field, the origin of livestock in the meat market, the origin of crops, vegetables, flowers, trees, etc. (origin and origin) Methods are becoming increasingly important in maintaining consumer food safety and label reliability.
[0009]
On the other hand, when collecting tissue and gene samples of individuals, methods of collecting hair with hair roots from the animal body with adhesive tape, methods of collecting blood, and, in the case of cattle, ear tags for individual identification Methods such as collecting the ears themselves at a slaughterhouse are used. In other words, the collection of individual identification sheets and tissues is treated as separate entities, and sufficient measures are taken against the collection of tissues, including contamination of tissues, hair, saliva, etc. from other individuals. Did not. Similarly, for a plant body, a method is used in which a plant body such as a leaf and a stem is cut off and collected, separately from mounting the identification tag.
[0010]
Japanese Patent Application Laid-Open No. 2001-114601 discloses a "method of storing DNA as genetic information" as a first example of the prior application similar to the present invention in the technical field. In Inventive Example 1, the DNA collected from human cells is designed to withstand long-term storage, and is stored and stored by a person in a pendant or the like. According to the document, the DNA useful as genetic information can be stored by the person in a portable or stationary form.
[0011]
Japanese Patent Application Laid-Open Publication No. 2000-151187, entitled "Method for Preserving Gene and Gene Preservation Display Used in This Method", is Japanese Patent Application Laid-Open No. 2000-151187. In Inventive Invention Example 2, a sample containing a gene is collected from a predetermined part of the body, applied to a gene-fixing paper and dried, and then the gene-fixing paper is stored at a predetermined location on the tablet.
[0012]
[Problems to be solved by the invention]
However, these methods have the following problems.
1) In the case of an animal body, a gene contained in saliva or the like of another individual attached to the body surface, or a tissue, saliva, blood or other gene of another individual attached to a human hand operating an adhesive tape, a brush or the like. There is a risk of tissue contamination.
[0013]
The incorporation of a tissue containing a gene from another individual has a risk of making the test itself impossible in an individual identification test using gene analysis. This danger is due to the fact that the results of the gene sequence collation no longer show identity, because the genes of other individuals are mixed.
[0014]
2) When tissue is excised with a knife or the like, when the same knife is used for sampling of another individual, if the tissue, blood, etc. of another individual is not removed by sufficient washing, separate blood , There is a risk of contamination of tissues of individuals other than the collected specimen due to adhesion of body fluids and tissue fragments.
[0015]
3) Since the identification slip and the sample are separately collected and stored, there is a possibility that the identification number of the individual identification slip of the artificial sample may be incorrect.
4) When the ear with the ear tag (identification tag) is collected as a tissue specimen at the slaughterhouse, the collected specimen becomes large in addition to the contamination of other individual tissues from the knife for resection used above. A large amount of space is required for sample preservation, which increases costs and labor. In addition, preservation of the specimen after cutting the ear requires avoiding contact with other specimens, and a great deal of labor is required to isolate, separate, and preserve the specimen.
[0016]
5) It is necessary to attach the identification tag to wild animals in the shortest possible time in order to minimize the burden on the individual. Complicated labor is required.
[0017]
6) Except for direct sampling by researchers, such tissue samples are left to general farmers and slaughterhouse workers. Thus, high levels of scientific knowledge of genetic contamination and contamination of other tissues cannot be expected, and there is a danger as to whether proper sample collection will continue.
[0018]
For example, regarding the above-mentioned individual cattle, after the cattle that has been raised (individual number is described in the ear tag) and processed at the slaughterhouse, the meat sold in the general market is the same or scientifically determined. If the cow does not hold a genetic sample for the individual before it is processed at the slaughterhouse, verification by actual genetic analysis should be performed even if the meat for verification is obtained from the market. Can not.
[0019]
7) In the protection of rare wild animals, identification tags for identification of individuals have been used for the purpose of tracking individual individuals. When studying the ecosystems and pedigrees of an animal, the collection of tissue gene specimens at the same time as identification tags is an important research issue. It may also be necessary to keep genetic samples of protected wild plants, for which collection is prohibited, to prevent illegal sale in the market.
[0020]
According to the present invention, when an identification tag for individual identification is attached to an animal or plant individual, a tissue sample of the individual is simultaneously collected and stored in a storage container, and once the identification tag is attached and the tissue sample is collected. It is an object of the present invention to provide a tissue sample collecting instrument, a tissue storage container integrated identification tag, and a unique identification tag mounting method which can be carried out at any time.
[0021]
[Means for Solving the Problems]
In order to achieve the above object, the tissue sample collecting device of the present invention has a structure in which the center portions of two handles are connected to each other, and the other end is movable by gripping one end, and the other end is movable. A penetrating body penetrating a tissue of a part of an animal or a plant at a first tip, a penetrating body at a second tip of the other end, and cutting out a part of the tissue of the animal or a plant by the penetrating part; A tissue collection container section for accommodating a container for accommodating the tissue, wherein the tissue cut out can be accommodated in a container connected to an identification tag on which an identification code for identifying an animal or plant is described. Features.
[0022]
Further, the penetrator is configured by a tissue tag upper support rod that supports the identification tag upper united with the tissue tag fixing projection for penetrating the tissue of a part of the animal and plant body, the tissue collection container portion, The tissue collection container for accommodating the cut tissue and the tissue collection container below the identification tag connected to the tissue collection container may be configured to be attachable.
[0023]
The tissue storage container integrated identification tag of the present invention is provided with a penetrating body penetrating a part of the animal and plant body at the tip, an identification tag upper part for writing an identification code for identifying the animal and plant, and the animal and plant by the penetrating body. It is characterized by comprising a container for accommodating the tissue obtained by cutting off a tissue of a part of the body, and an identification tag lower part in which an identification tag for identifying an animal and a plant is described and a lower identification tag connected thereto. And
[0024]
Further, the penetrator is integrally formed with a tissue tag fixing projection for penetrating a part of the tissue of the animal and plant, and the tip of the tissue tag fixing projection seals a container containing the cut tissue. It is good to equip with the sealing lid for the sake.
[0025]
The unique identification tag mounting method of the present invention is an operation step of operating an identification tag mounting device in which the central portions of two handles are connected to each other and one end is gripped, and the other end is movable. A penetrating body penetrating a part of the tissue of the animal or plant body at the first end of the other end, and a penetrating body at the second end of the other end, and A tissue collecting step of accommodating the tissue obtained by cutting out a part of the animal or plant body by the body, and housing the cut out tissue in a container connected to an identification tag on which an identification code for identifying the animal or plant is written. Can be executed efficiently.
[0026]
BEST MODE FOR CARRYING OUT THE INVENTION
Next, with reference to the accompanying drawings, embodiments of a tissue sample collecting instrument, a tissue storage container integrated identification tag and a unique identification tag mounting method according to the present invention will be described in detail. Referring to FIGS. 1 to 6, there is shown an embodiment of a tissue sample collecting instrument, a tissue storage container integrated identification tag and a unique identification tag mounting method of the present invention.
[0027]
In order to solve the above-mentioned conventional problems, the tissue sample collecting instrument, the tissue storage container integrated identification tag and the unique identification tag mounting method of the present invention integrates a container for collecting and storing a tissue sample into the identification tag. At the same time as attaching the identification card, the tissue specimen was collected in the storage container. At the same time, the device for attaching the identification tag was specially designed to appropriately hold the identification tag and the tissue sample collection / preservation container, and to easily attach the identification tag. Furthermore, the same symbol is displayed on the identification tag and the collection storage container to ensure that the collected tissue specimen matches the identification tag. The details of these components will be described below.
[0028]
FIG. 1 is a side view showing a configuration example of a tissue sample collecting instrument based on an embodiment of the present invention. In FIG. 1, the tissue sample collecting instrument of the present embodiment is configured in a punch shape, and includes a grip handle 1, an identification tag upper support bar 2, and a lower support portion 3. Note that a collection container mounting hole 31 is formed inside the lower support portion.
[0029]
The grip handles 1a and 1b having the above configuration are configured to be movable in the direction indicated by the dotted line around the fulcrum by gripping the upper and lower handles. By this movement, the identification tag upper support bar 2 can be inserted and moved into the collection container mounting hole 31 formed inside the lower support portion.
[0030]
FIG. 2 is a conceptual side view for explaining an example of use of the tissue sample collecting instrument shown in FIG. Further, FIG. 2 is a diagram for explaining an example of a method of mounting the identification tag and the unique identification tag integrated with the tissue storage container using the tissue sample collecting instrument of FIG. The tissue storage container-integrated identification tag of the embodiment is applied to the tissue sample collecting instrument shown in FIG. 1 and constitutes an example of a unique identification tag mounting method. The tissue storage container-integrated identification tag of the embodiment shown in FIG. 2 includes the identification tag upper portion 41, the identification tag lower portion 42, the tissue collection container 45, and the identification tag lower holding portion 43 (when attached) 43. You. FIG. 2 shows an example of a state in which the animal and plant tissue 5 to be collected is sandwiched between the upper part 41 of the identification tag and the lower part 42 of the identification tag.
[0031]
3 and 4 are diagrams showing in more detail the configuration contents of the tissue storage container integrated identification tag and the unique identification tag mounting method. In the example of the procedure of the unique identification tag mounting method, FIG. 3 shows a state example before the mounting operation of the tissue storage container integrated identification tag and FIG. 4 shows a state example after the mounting operation of the tissue storage container integrated identification tag. I have.
[0032]
In FIG. 3, which shows the state before the mounting operation of the identification tag integrated with the tissue storage container, the upper and lower identification tags 41 and the identification tag lower portion 42 sandwich the animal and plant tissue 5 to be collected. An identification tag fixing protrusion 411 is formed on the identification tag upper portion 41. A tube sealing lid 44 is formed at the tip of the identification tag fixing projection on the identification tag upper part 41.
A tissue preservation tube 45 is provided below the identification tag 42.
[0033]
The state in which the collected animal and plant tissue 5 shown in FIG. 3 is sandwiched between the identification tag upper portion 41 and the identification tag lower portion 42 is changed from the state after the mounting operation of the tissue storage container-integrated identification tag in FIG. An example of the operation procedure is shown below.
The protruding portion 411 of the identification tag 41 penetrates the tissue body 5 and pushes the collected animal and plant tissue 51 into the tissue preservation tube 45 attached to the lower portion (receiver) 42 of the tissue tag. At the same time, the tube sealing lid 44 seals the container of the tissue preservation tube 45. The organization sheet itself is fixed by the projection 411 for fixing the organization sheet. It is desirable that the tissue forms 41 and 42 are made of a flexible material such as polyurethane.
[0034]
In the above embodiment, the storage tube 45 may be prepared in various sizes according to the required amount of the collected animal and plant tissue 51. If the size of the storage tube 45 is set to a 96-well 0.5 ml diameter, it can be mounted on a plate for a general-purpose automatic analyzer.
In the identification tag integrated with the tissue storage container of the present embodiment, the identification symbol, barcodes 46 and 47, etc. are printed and displayed on the identification tag surface, and the same symbol and number are displayed on the specimen storage tube 45 as one set. Good to do.
[0035]
5 and 6 show examples of the configuration of the identification tag 4. 5 and 6 showing two types of configuration examples, the plan contents are shown by a plan view (a) and a side view (b).
FIG. 5 is an example of a configuration in which a notch 412 for cutting is formed at the center of the identification tags 41a and 42a, and the upper and lower identification tags 41a and 42a are integrated. FIG. 6 shows a configuration example in which the planar structure is circular.
In a general-purpose identification tag format (for wildlife, livestock such as pigs, and plants), the size of the identification tag may be prepared according to the application.
[0036]
In the present invention, an identification tag processed into an arbitrary shape can be used, and different identification tags such as an ear tag for cattle, an identification tag for pigs, and an identification tag for horses and chickens can be used. In addition, by changing the collection / storage container according to the capacity of the tissue specimen to be collected, it becomes possible to appropriately collect samples necessary for research and inspection.
[0037]
When a support sample for fixing the identification tag penetrates an animal or plant (for example, an ear or the like) at the time of attaching the identification tag, the tissue sample collected according to the present invention may have a sample collection and a container sealing lid at its tip. The inserted cap inserts the tissue into the storage container.
At the same time when the identification tag is fixed, the tissue sample is collected in the storage container, and the sealing lid is in close contact with the storage container, and the tissue sample is sealed in the storage container. Thereafter, by removing the storage container, a tissue specimen with the same identification symbol as the identification tag is collected.
[0038]
In this series of processes, the operator does not touch the tissue specimen. Also, any instrument used to collect a tissue sample from another individual during collection of the tissue sample does not touch the tissue sample. For this reason, the collected tissue specimen is free from the possibility of contamination of the tissue of another individual.
The storage container is sealed, and the same identification symbol as that of the individual is displayed. Therefore, even when tissue samples from a plurality of individuals are continuously collected, there is no danger that the samples will be mistaken.
[0039]
In addition, even if there is no scientific knowledge about the collection of the sample and the prevention of contamination of the sample with genes and tissues from other individuals, the present invention allows the sampler to touch the tissue to be collected. There is no need to re-use the equipment for tissue collection. Therefore, sampling can be performed in an appropriate and stable state.
The present invention is effective not only for collecting a tissue sample from a living body but also for collecting a tissue sample from carcass in a slaughterhouse.
[0040]
When a slaughterhouse accepts an individual already equipped with an ear tag for individual identification and collects and saves a tissue specimen of the individual, the identification with the "tissue preserved" and the ear tag number obtained at the time of arrival is also described. If the present invention using a vote is used, a completion indication that the tissue has been preserved and the target tissue specimen can be collected from the carcass without being confused with other individuals. Furthermore, compared to the method of removing and storing the ear itself with the ear tag attached, the amount of the sample is small, and since it is sealed in a container suitable for storage, contamination of blood and hair from other individuals and storage space Can be reduced.
[0041]
Furthermore, since the tissue specimen collected at the time of specimen collection is stored in the storage container as it is when the identification tag is attached, there is no need to worry about the tissue of other individuals accidentally entering and contamination of external substances, Tissue samples can be collected and stored in an appropriate state.
Further, by displaying the same identification symbol as the identification tag on the storage container in advance, it is possible to prevent the collected sample from being mistaken for a tissue sample of another individual.
[0042]
The above embodiment is an example of a preferred embodiment of the present invention. However, the present invention is not limited to this, and various modifications can be made without departing from the scope of the present invention.
[0043]
【The invention's effect】
As is clear from the above description, the tissue sample collecting instrument, the tissue storage container integrated identification tag and the unique identification tag mounting method of the present invention have the center portions of the two handles connected to each other, and the one end side is connected to the two handles. By grasping, the other end side is movable, the first end of the other end penetrates a part of the tissue of the animal or plant, and the second end of the other end receives the penetrator, Further, the tissue obtained by cutting out a part of the tissue of the animal or plant body by the penetrating portion is accommodated. As a result, the cut tissue can be efficiently accommodated in the container connected to the identification tag on which the identification code for identifying the animal or plant is written.
[Brief description of the drawings]
FIG. 1 is a side view showing a configuration example to which an embodiment of a tissue sample collecting instrument, a tissue storage container integrated identification tag, and a unique identification tag mounting method of the present invention is applied.
FIG. 2 is a conceptual side view for explaining an example of use of the tissue sample collecting instrument shown in FIG.
FIG. 3 is a diagram showing in more detail the configuration contents of a tissue storage container integrated identification tag and a unique identification tag mounting method.
FIG. 4 is a diagram showing in more detail the configuration of a tissue storage container integrated identification tag and a unique identification tag mounting method.
FIG. 5 shows a first configuration example of an identification tag.
FIG. 6 shows a second configuration example of the identification tag.
[Explanation of symbols]
REFERENCE SIGNS LIST 1 grip handle 2 identification tag upper support rod 3 lower support portion 5 animal and plant tissue to be collected 31 collection container mounting hole 41 identification tag upper 42 identification tag lower 43 identification tag lower holder (when mounted)
44 Tube lid 45 Tissue collection container (Tissue preservation tube)
46, 47 Barcode 51 Sampling animal and plant tissue 411 Projection for fixing identification tag

Claims (7)

The central portions of the two handles are connected to each other, and by gripping one end, the other end is made movable,
A penetrator penetrating a tissue of a part of the animal or plant body at the first end of the other end;
A tissue collection container unit that receives the penetrator at the second end of the other end, and stores a container for accommodating the tissue obtained by cutting out a part of the tissue of the animal or plant by the penetrating unit; With
A tissue sample collecting instrument, wherein the cut tissue can be accommodated in the container connected to an identification tag on which an identification code for identifying the animal or plant is written. 2. The tissue penetrating body according to claim 1, wherein the penetrating body is constituted by a tissue tag upper support rod that supports an identification tag upper part integrated with a tissue tag fixing protrusion for penetrating a part of the tissue of the animal or plant body. The tissue sample collecting device according to the above. The tissue collection container section is configured to be capable of mounting the tissue collection container below the identification tag connected to the tissue collection container and the tissue collection container for accommodating the cut tissue. The tissue sample collecting device according to claim 1. An identification tag top for describing an identification code for identifying the animal or plant with a penetrating body penetrating a part of the animal or plant body at the tip,
A container for accommodating the tissue obtained by cutting out a part of the animal and plant body by the penetrator, and an identification tag lower portion in which an identification tag with an identification code for identifying the animal and plant is connected. A tissue preservation container-integrated identification tag characterized by comprising: The tissue storage container-integrated identification tag according to claim 4, wherein the penetrating body is integrally formed with a tissue tag fixing projection for penetrating a part of the tissue of the animal or plant body. The tissue storage container-integrated identification according to claim 5, wherein a sealing lid for sealing the container accommodating the cut tissue is provided at a tip end of the tissue tag fixing projection. Votes. An operation step of operating an identification tag mounting device in which the center portions of the two handles are connected to each other, and the other end side is movable by grasping one end side;
A penetrating body penetrating a part of the tissue of an animal or plant at the first end of the other end in the operation step, and receiving the penetrating body at the second end of the other end, and A tissue collecting step of accommodating the tissue obtained by cutting off a part of the tissue of the animal or plant by a body,
A method for mounting a unique identification tag, wherein a procedure for accommodating the cut tissue in the container connected to an identification tag on which an identification code for identifying the animal or plant is described can be efficiently performed.

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