JP2004093312A5 - - Google Patents

Claims (28)

  A method for detecting the presence or absence of cells in a test solution, which uses an imaging means to capture an image of the test solution, measure a color signal in a certain range in the captured image, and the measurement results are similar in advance. A cell detection method comprising comparing a measurement result of a sample that does not include cells measured in step 1 or a measurement result of a sample with a known cell number. 2. The cell detection method according to claim 1, wherein the value of the color signal measured in a certain range of the test solution is collated with a calibration curve indicating the relationship between the color signal and the number of cells prepared in advance. 3. The cell detection method according to claim 2, wherein the number of cells in the test solution is detected. The cell detection method according to claim 1, wherein an image of the test solution is captured using an imaging means through the transparent sample support. The sample support has a flow path composed of a groove or a hole, and a test solution is continuously or intermittently flowed in the flow path, and an image of the flowing test solution is captured using an imaging means. The cell detection method according to claim 4.   The color signal format to be measured is RGB color system, XYZ color system, X10Y10Z10 color system, Yxy color system, UCS color system, Luv color system, L * u * v * color system, LAB table. Color system, L * a * b * color system, CMYK color system, Munsell color system, Modified Munsell color system, Ostwalt color system, HSV color system, HSB color system, HSL color system, HLS The cell detection method according to claim 1, wherein the cell detection method is at least one selected from a color system, a YIQ color system, and a PCCS color system.   The cell detection method according to claim 1, wherein an average value or an integrated value of the measured color signals is calculated.   The cell detection method according to claim 1, wherein the sample containing cells is stained with a reagent or an antibody. The cell detection method according to claim 8, wherein the reagent used for staining is dithizone (diphenylthiocarbazone).   The cell detection method according to claim 1, wherein an image of a sample obtained by crushing cells is captured. The cell detection method according to claim 10 , wherein at least one selected from ultrasonic waves, enzymes, and surfactants is used in the crushing treatment. The image pickup means is a CCD (Charge-Coupled Dev).
ice) camera and CID (Charge Injection Device) camera,
The cell detection method according to claim 1, wherein the cell detection method is at least one selected from an RGB camera, a film camera, an instant camera, a C-MOS camera, and a video camera.   The cell detection method according to claim 1, wherein the captured image is captured in a computer.   The cell detection method according to claim 1, wherein the captured image is displayed on an image display unit.   The cell detection method according to claim 1, wherein the cells to be detected are cells derived from the pancreas. The cell detection method according to claim 15 , wherein the cell to be detected is Langerhans island.   The cell detection method according to claim 1, wherein the steps from imaging of a sample containing cells and measurement of a color signal from the captured image are automatically performed. Mounting means of the sample, imaging means for imaging a sample containing cells, and receives the image captured from the imaging unit, cell detection, characterized in that it comprises a measuring means to measure a range of the color signal in the image captured apparatus. The cell detection device according to claim 18, further comprising means for storing an image captured by the imaging unit. 19. The cell detection apparatus according to claim 18, wherein the sample setting means has a transparent sample support, and an image of the test solution is captured through the sample support using an imaging means. 19. The cell detection apparatus according to claim 18, wherein the sample support has a flow path composed of grooves or holes, and captures an image of a test solution flowing continuously or intermittently through the flow path. The cell detection device according to claim 18 , wherein the imaging means is at least one selected from a CCD camera, a CID camera, an RGB camera, a film camera, an instant camera, a C-MOS camera, and a video camera. The cell detection device according to claim 18, further comprising means for enlarging and imaging. The cell detection apparatus according to claim 18, further comprising means for crushing cells. The cell detection apparatus according to claim 24 , wherein the crushing means is an ultrasonic crushing apparatus. The cell detection device according to claim 18, further comprising means for displaying a captured image. The image display unit is a CRT display, a liquid crystal monitor, a PDP (Plasma Display Panel), a liquid crystal projector, an EL (Electro Luminescence) display, an FED (Field Emission Display), a VFD (Vacuum Fluorescent Display), or a DMr. The cell detection device according to claim 26, wherein: The cell according to claim 18, further comprising means for notifying when at least one value of a component constituting a color signal analyzed and measured from a captured image deviates from a predetermined range. Detection device.