JP2003337088A - Cell cleaning rotor and cell cleaning centrifugal machine provided with the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cell cleaning rotor capable of reliably supplying an equal quantity of a cleaning liquid for the inside of a large number of test tubes and can easily deal with an increase or a decrease in the number of the test tubes, and to provide a cell cleaning centrifugal machine provided with the same. <P>SOLUTION: The cell cleaning rotor comprises a rotor; a plurality of test tube holders capable of moving outward by a centrifugal force when the rotor is rotated; and a cleaning liquid distributing element for supplying the cleaning liquid for the inside of the large number of test tubes held by the test tube holders. By providing the cleaning liquid distributing element with a structure having a cleaning liquid inlet at its center part; distributing the cleaning liquid introduced from the inlet to the test tubes; and attaching a freely detachable filter to the cleaning liquid inlet, the cell cleaning rotor is provided with a structure capable of preventing foreign matter from blocking a channel, and the cell cleaning centrifugal machine using the same is provided. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cell washing centrifuge for washing living cells such as red blood cells by utilizing centrifugal force, and more particularly to a cell washing rotor used therein.

[0002]

2. Description of the Related Art Conventionally, for antiglobulin test, cross-match test, irregular antibody screening, etc. at the time of blood transfusion test, erythrocytes are washed with a washing solution such as physiological saline to remove excess antibody in the suspension. A process is required, and various cell washing centrifuges are known for this purpose.

For example, in Japanese Unexamined Patent Publication No. 50-22693, nozzles are installed radially from the outer periphery of the bottom surface of a conical container, and the cleaning liquid injected from the center of the cleaning liquid distribution element by centrifugal force due to rotation is equally divided. Disclosed is a cleaning liquid distribution element having a structure for distributing and supplying a cleaning liquid from a nozzle to a large number of test tubes held by a test tube holder.

Further, for example, Japanese Utility Model Laid-Open No. 2-81640 discloses a structure for supplying a cleaning liquid into a large number of test tubes held by a test tube holder from holes formed in a cleaning liquid distribution element.

[0005]

In order to perform a good blood transfusion test or the like by a centrifuge for the purpose of automating cell washing, the washing liquid distribution element supplies the same amount of washing liquid to a large number of test tubes. Is desirable. If there are variations in the amount of cleaning liquid supplied in multiple test tubes, for example,
In a test tube with a small amount of washing liquid supplied, a larger amount of foreign substances such as antibodies remain in the suspension than in other test tubes. On the contrary, in test tubes with a large supply of washing liquid, there are few residual foreign substances such as antibodies in the suspension, and this difference becomes the difference in the test results due to the reagent reaction performed in the subsequent process, and as a result, it can be used in the determination of blood transfusion tests. It causes a serious error.

Further, when there is a test tube with a small amount of cleaning liquid, if the cleaning liquid is also supplied to other test tubes in accordance with the amount of the cleaning liquid supplied to this test tube, an excessive amount of cleaning liquid is supplied to the other test tubes and the cleaning liquid is supplied. A large amount overflows the test tube, causing a problem of losing a valuable cell sample. Furthermore, if the number of washings is determined according to the amount of washing liquid, the washing process will take a long time.

There are several possible reasons why the amount of cleaning liquid supplied to the test tube becomes non-uniform. The first cause is due to the non-uniformity of the flow path resistance of the cleaning liquid distribution element. For example, in the cleaning liquid distribution element shown in Japanese Utility Model Laid-Open No. 2-81640, which supplies a cleaning liquid into a large number of test tubes held by a test tube holder through holes that are radially drilled, holes are drilled or the like. Difficult except for machining. However, the machining of holes is
Errors easily occur in the shape of the entrance and exit of the hole and the roughness of the inner surface of the hole,
This causes non-uniform flow path resistance, resulting in variations in the amount of cleaning liquid supplied into a large number of test tubes.

Japanese Patent Laid-Open No. 50-22693 discloses a structure in which a metal pipe is embedded in a cleaning liquid distribution element and the cleaning liquid is supplied into a large number of test tubes with the metal pipe serving as a flow path. In this case as well, a processing error occurs in the processing shape of the pipe end surface and the pipe length, which causes uneven flow resistance as described above, resulting in variations in the amount of cleaning liquid supplied into a large number of test tubes.

The second cause of non-uniformity of the cleaning liquid is leakage of the cleaning liquid. For example, if the distance between the cleaning liquid outlet of the cleaning liquid distribution element and the test tube opening is large, the cleaning liquid from the liquid distribution element may not completely enter the test tube due to an error in the drilling direction. This causes variation in the amount of the cleaning liquid supplied.

Further, when the tip of the metal pipe is installed near the test tube so that the cleaning liquid completely enters the test tube, the variation in the length dimension of the test tube and the rotation of the test tube holder in the lateral direction at the time of rotation start. The test tube and the tip of the metal pipe come into contact with each other,
Problems that cause damage to test tubes occur.

The third cause of non-uniformity of the cleaning liquid amount is due to foreign matter in the cleaning liquid. While the cleaning liquid sent out by the pump reaches the liquid distribution element, it traps dust and cotton dust in the air, and when these foreign substances become clogged in the holes and pipes of the cleaning liquid distribution element, the flow path is blocked, and as a result Reduce the wash solution supply to the corresponding test tube. In addition, when physiological saline or the like is used as the cleaning liquid as a cause of the foreign matter, solid substances such as sodium chloride deposited in the tank or the flow path may block the flow path and cause a decrease in the supply amount of the cleaning solution.

In the above-mentioned cleaning liquid distribution element, it is not possible to see a foreign substance that blocks such a flow path from the outside, so that the cleaning process is temporarily stopped, and the amount of cleaning liquid distributed to the test tube is complicated to check. Necessary work. Furthermore, in the above-described cleaning liquid distribution element, the cleaning liquid is always supplied by the number of holes or pipes regardless of the increase or decrease in the number of test tubes to be processed. Therefore, when the number of test tubes is small, the cleaning liquid is wasted.

In view of the above-mentioned problems of the prior art, it is an object of the present invention to provide a cleaning liquid distribution element capable of supplying a uniform amount of cleaning liquid into a large number of test tubes.

Another object of the present invention is to provide a cell washing rotor which has good washing characteristics and high reliability by using the washing liquid distribution element as described above, and can easily cope with an increase or decrease in the number of test tubes, and a cell washing rotor. It is to provide a cell washing centrifuge equipped with the same.

[0015]

In order to achieve the above object, the present invention provides a rotor and a plurality of test tubes which are rotatably mounted on the rotor and can be moved outward by centrifugal force when the rotor rotates. In a cell washing rotor comprising a holder and a washing liquid distribution element that is attached to the rotor and rotates together with the rotor, and supplies a washing liquid into a large number of test tubes held by the test tube holder, the washing liquid distribution element is provided at a central portion. One of the features is that it has a cleaning liquid introducing hole, distributes the cleaning liquid introduced from the introducing hole to the test tube, and attaches a detachable filter to the cleaning liquid introducing hole.

Another feature of the present invention is to provide a rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and the rotor mounted on the rotor. In a cell washing rotor comprising a washing liquid distribution element that rotates together with the washing liquid and supplies the washing liquid into a large number of test tubes held by the test tube holder, the washing liquid distribution element has a circular flat plate-shaped upper portion having a washing liquid introduction hole in a central portion thereof. It is composed of a distribution element and a circular plate-shaped lower distribution element, and has a plurality of radial grooves in one or both of the upper distribution element and the lower distribution element, and a removable filter is attached to the cleaning liquid introduction hole. It is in.

Another feature of the present invention is that a rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and the rotor mounted on the rotor. In a cell washing rotor that rotates together with the washing liquid distribution element that supplies a washing liquid into a large number of test tubes held by the test tube holder, the washing liquid distribution element has a conical shape having a washing liquid introduction hole in a central portion. An upper distribution element comprising a first portion and a circular flat plate-shaped second portion around the first portion; a conical third portion facing the first portion; and a second portion. 4th, which is arranged in a position facing
And a lower distribution element composed of a first part and a third part, which forms a flow path of the cleaning liquid in the gap between the first part and the third part, and is provided in one or both of the second part and the fourth part. The distribution channel of the cleaning liquid is formed by the groove and the removable filter is attached to the cleaning liquid introducing hole.

Another feature of the present invention is to provide a rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and the rotor mounted on the rotor. In a cell washing rotor that rotates together with the washing liquid distribution element that supplies a washing liquid into a large number of test tubes held by the test tube holder, the washing liquid distribution element has a conical shape having a washing liquid introduction hole in a central portion. An upper distribution element including a first portion and a second portion having a circular flat plate shape around the first portion, and a central distribution element including a circular flat plate having a central portion arranged so as to face the second portion. And a second distribution element of the upper distribution element, the lower distribution element having a conical shape and arranged at a position facing the first section to form a fluid passage between the first distribution section and the first section. Under And one or with both in providing a plurality of radial grooves on the upper surface of the central portion dispensing element, lies in the mounted freely filter removably attached to the cleaning liquid introduction hole. With the above configuration, it is possible to prevent foreign matter from blocking the flow path and causing the cleaning liquid flowing into the test tube to become nonuniform.

[0019]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view illustrating a structure and a component configuration of a cell washing rotor showing an embodiment of the present invention. In this embodiment, the cell washing rotor 1 is composed of a rotor 2 and a washing liquid distribution element 5, and the disk-shaped rotor 2 has 24 outer circumferences.
24 square tube holes 3 are placed in this hole.
Is installed. Test tube holder 3 with test tube 4 inserted
When the rotor 2 rotates, it can freely move in the horizontal direction by the centrifugal force about the mounting portion. In this embodiment, the rotor 2
The test tube holder 3 is manufactured by pressing a stainless plate, but a resin molded product can also be manufactured. A cleaning liquid distribution element 5 for supplying a cleaning liquid to the test tube 4 is mounted on the rotor 2. The cleaning liquid distribution element 5 rotates with the rotor 2 because the lower convex portion thereof engages with the elongated hole of the rotor 2. Further, even if the cleaning liquid distribution element 5 is attached to and detached from the rotor, the positional relationship between the cleaning liquid distribution element 5 and the rotor 2 and the test tube holder 3 does not change.

FIG. 2 is a perspective view for explaining the structure and component structure of the cleaning liquid distribution element according to the embodiment of the present invention. In the present embodiment, the cleaning liquid distribution element 5 is composed of an upper distribution element 6 and a lower distribution element 8, and the lower distribution element 8 is formed with 24 grooves 9 radially corresponding to the number of the test tubes 4.
By arranging the upper distribution element 6 and the lower distribution element 8 in combination, the space formed by the groove 9 of the lower distribution element 8 and the flat portion of the upper distribution element 6 becomes the flow path of the cleaning liquid. According to this embodiment, by making the cleaning liquid flow path surrounded by the groove and the plane, the upper distribution element 6 and the lower distribution element 8 can be manufactured only by resin molding. By resin-molding the upper distribution element 6 and the lower distribution element 8 using a high-precision mold, the shape of the groove 9 of the lower distribution element 8 becomes uniform with high accuracy in the same cleaning liquid distribution element 5, and the performance is improved. It has become possible to mass-produce the cleaning liquid distribution element 5 having a uniform flow resistance at any time.

In the above embodiment, the groove may be provided in one or both of the upper distribution element 6 and the lower distribution element 8, or these may be made of ceramic or the like instead of resin. Considering the ease of fabrication and the uniformity of flow path resistance, it is preferable to provide a groove in the lower distribution element 8 and use a space formed between the lower distribution element 8 and the plane of the upper distribution element 6 as a flow path as in the above embodiment. The best.

Further, in FIG. 2, a cylindrical grasping portion 14 is provided at the center of the upper distribution element 6. Grasping part 14
A plurality of anti-slip concave portions 26 and anti-slip convex portions 27 are provided in the vertical direction on the outer periphery of the. This is convenient when the rotor 1 is manually manually rotated in small increments after the cell washing step to satisfactorily mix the red blood cells and the reagent in the test tube 4 to promote the reaction.

FIG. 10 shows a cell washing centrifuge 20 according to the present invention.
FIG. 3 is a vertical sectional view showing the overall configuration of The function of the cleaning liquid distribution element 5 will be described in more detail with reference to FIG. In FIG. 10, the cell washing rotor 1 is attached to the drive shaft 22 of the motor 21. A drive voltage is applied to the motor 21 from a drive circuit (not shown), and the drive shaft 22 is rotated. The cell washing rotor 1 is rotationally driven by a drive shaft 22, and the test tube holder 3 and the test tube 4 containing an appropriate amount of living cells such as red blood cells in advance are horizontally swung by centrifugal force.

At this time, the pump 23 is operated to feed the washing liquid from the external washing liquid tank to the nozzle 25 located at the upper part of the cell washing centrifuge 20, and the washing liquid is ejected downward from the nozzle 25 to rotate the washing cells. It enters into the cleaning liquid distribution element 5 through the cleaning liquid introduction hole 7 in the center of the rotor 1. The cleaning liquid that has entered the cleaning liquid distribution element 5 is moved to the outer periphery by centrifugal force, and is divided into 24 channels constituted by the groove 9 of the lower distribution element 8 and the flat portion of the upper distribution element 6 to flow into the cleaning liquid distribution element. The element 5 vigorously jumps out from the outer circumference. The washed-out washing solution hits the inner wall of the test tube 4 located outside the washing solution distribution element 5, travels along the wall surface, and the living cells at the bottom of the test tube 4 are suspended to create a suspended state. When an appropriate amount of cleaning liquid enters the test tube 4, the pump 23 stops and the cleaning liquid injection process ends.

Subsequently, the rotation is continued until the floating living cells gather at the bottom of the test tube 4. After that, the rotation is stopped and the test tube holder 3 is returned to the vertical position. Next, in this embodiment, the motor 21 is operated at a low speed while the magnetic element 28 as a means for restraining the horizontal movement of the test tube holder 3 is functioning.
To rotate. Test tube holder 3 is made of magnetic material SUS430
Since it is made of a material or the like, it is attracted by the magnetic force of the magnetic element 28 and rotates while maintaining the vertical state, so that the cleaning liquid in the test tube 4 is discharged to the outside by centrifugal force. Living cells at the bottom of the test tube 4 remain as they are, and only the washing solution is lost.

By repeating these steps, the cell washing centrifuge 20 separates and removes foreign substances such as antibodies from the living cells in the test tube 4. Since the washing process is carried out by such a process, it is desirable for the performance of the cell washing centrifuge 20 that the washing liquid supplied into a large number of test tubes is equal in volume.

According to the embodiment of FIG. 2, the cleaning liquid distribution element 5
Since the upper distribution element 6 and the lower distribution element 7 are made of a high-precision resin molded product, the shape of the groove 9 of the lower distribution element 8 is higher than that of a hole formed by punching or a flow path of a metal pipe. Can be uniform. Therefore, it is possible to mass-produce the cleaning liquid distribution element 5 with good reproducibility and capable of distributing an equal amount of cleaning liquid with a uniform flow resistance as compared with the perforated hole or the flow path of the metal pipe. Becomes

After the cell washing step of the cell washing centrifuge 20, for example, after washing the red blood cells, a reagent such as antiglobulin serum is dropped into the test tube 4, and the cell washing rotor 1 is manually rotated in small forward and reverse directions. It is necessary to mix the red blood cells in the test tube 4 and the reagent well to promote the reaction.
In the embodiment of FIG. 2, the grasping portion 14 is provided at the center of the upper distribution element 6.
Further, by providing the anti-slip concave portion 26 and the anti-slip convex portion 27 on the outer circumference, it is easy to manually rotate in forward and reverse directions in small steps, and there is an effect that a reliable reagent reaction can be promoted.

FIG. 3 is a plan view of a cell washing rotor according to an embodiment of the present invention. When the rotation speed of the motor 21 is increased in order to shorten the processing time of the cell washing centrifuge 20, the flight direction is bent by the wind pressure of the rotation until the washing solution that has jumped from the outer periphery of the washing solution distribution element 5 enters the test tube 4, and is completely In some cases, it may not enter the test tube 4. In the embodiment of FIG. 3, the groove 9 is provided at a position where the center line 10 of the groove 9 is advanced in the rotation direction of the rotor 2 rather than the straight line connecting the center of the test tube holder 3 and the rotation center of the rotor 2. . By doing so, the bending of the cleaning liquid in the flight direction can be corrected, and the cleaning liquid can be reliably injected into the test tube 4 as shown in the figure. The advancing angle depends on the rotation speed of the rotor 2 and the distance between the cleaning liquid distribution element 5 and the test tube 4, but is preferably 0.5 to 5 degrees, and more preferably 1 to 3 degrees.

FIG. 4 is a plan view of a cell washing rotor according to an embodiment of the present invention. The center line 10 of the groove 9 is a straight line or a curved line and is installed at an angle with a straight line passing through the center of the rotor. There is. Also in this example, the bending of the cleaning liquid in the flight direction can be corrected by advancing the angle formed by the center line 10 of the groove and the straight line passing through the center of the rotor in the rotation direction of the rotor 2.

FIG. 5 is a plan view of the lower distribution element 8 according to an embodiment of the present invention. In this embodiment, of the groove 9,
The groove end 11 on the center side of the rotor 2 is formed by a smooth curved surface. With such a shape, it is possible to suppress variations in flow path resistance when the cleaning liquid enters the cleaning liquid distribution element 5 through the cleaning liquid introduction hole 7 and flows into the plurality of grooves 9. Particularly, when the adjacent groove end portions 11 are formed by semicircular arcs as in this embodiment, it is effective in reducing the variation in the flow path resistance.

5 and 2, the upper distribution element 6
Further, the lower distribution element 8 is provided with a convex portion 15 at the outermost periphery thereof so that the portion where the groove 9 is arranged is convex outwardly than the other portions. With such a shape, the distance at which the test tube 4 and the cleaning liquid distribution element 5 interfere can be reduced. As a result, the distance between the cleaning liquid distribution element 5 and the test tube 4 can be set small, and the direction of flight is bent by the wind pressure of the rotation until the cleaning liquid jumping out from the convex portion 15 enters the test tube 4, and inside the test tube 4. The trouble of not entering has also decreased. Further, it is possible to delete a useless portion on the outer periphery of the cleaning liquid distribution element 5, and as a result, the cleaning liquid distribution element 5 can be removed.
By reducing the weight of, the load on the motor 21 can be reduced.

FIG. 6 is a sectional view of a cleaning liquid distribution element 5 according to an embodiment of the present invention. A porous filter 12, which is removable from above, was attached to the cleaning liquid introduction hole 7 located at the center of the upper distribution element 6. With such a configuration, the foreign matter in the cleaning liquid can be removed before entering the cleaning liquid distribution element 5, clogging the groove 9 and closing the flow path, and as a result, the amount of the cleaning liquid supplied to the test tube 4 is reduced. It was possible to prevent it.
In this embodiment, the porous filter 12 is a polypropylene resin sintered molded product having a filtration accuracy of 50 μm.
It was possible to replace with a new one before the flow rate decreased when the filter was clogged. In addition to this, the porous filter 12
Is practically no problem even if it is manufactured with a screen mesh made of metal such as stainless steel.

In FIG. 6, the upper distribution element 6 is entirely made of a transparent material. As a result, the groove 9 of the lower distribution element 8 can be visually observed from above, and even if the flow path is blocked by a foreign substance in the cleaning liquid, its position can be instantly specified and an early countermeasure can be taken. If the groove 9 is observable, the same effect can be obtained even if only a part of the upper distribution element 6 is made of a transparent member.

FIG. 7 is a perspective view illustrating a structure and a component structure of a cleaning liquid distribution element according to another embodiment of the present invention. Although the above-described cell washing rotor 1 holds 24 test tube holders 3, the washing process of 24 test tubes 4 is not necessarily performed in one washing process, and in some cases, 12 test tubes 4 are held. May be processed. In this case, since the cleaning liquid is supplied and injected also to the portion without the test tube 4, the cleaning liquid is wasted.

In the embodiment shown in FIG. 7, the cleaning liquid distribution element 5 is used.
Is composed of an upper distribution element 6, a lower distribution element 8 and a plug member 16, and the plug member 16 is an upper distribution element 6 and a lower distribution element 8.
It is structured such that it is held between and is in close contact with and engaged with the groove end portion 11. As a result, it was possible to completely stop the flow of the cleaning liquid in the groove 9 with which the plug member 16 was engaged. Therefore, in the cell washing rotor 1 having 24 test tube holders 1,
If the cleaning process is performed using two test tubes 4,
Plug members 16 are provided at twelve groove end portions 11 where no
By tightly engaging with each other, it is possible to stop the wasteful supply of the cleaning liquid portion. Of course, it is desirable to install the plug member 16 at a position symmetrical with respect to the center of the cell washing rotor 1. Furthermore, since the upper distribution element 6 is partially or wholly formed of a transparent member, the position of the plug member 16 can be confirmed from above, and the test tube 4 can be reliably installed at the cleaning liquid supply point. Further, by forming the plug member 16 from an elastic material such as silicon rubber, it is possible to further improve the adhesion to the groove end portion 11 and reliably stop the flow of the cleaning liquid.

FIG. 8 is a perspective view illustrating a structure and a component structure of a cleaning liquid distribution element according to another embodiment of the present invention. In the embodiment in FIG. 8, the cleaning liquid distribution element 5 comprises an upper distribution element 6, a lower distribution element 8 and a central distribution element 17 in which grooves 9 are formed. With this configuration,
The cleaning liquid distribution element 5 can easily cope with an increase or decrease in the number of test tube treatments by sequentially exchanging the central part distribution element 17 having different numbers of grooves, and as a result, it is possible to surely eliminate the waste of the cleaning liquid part.

FIG. 9 is a cross-sectional view of the cleaning liquid distribution element 5 of this structure. The central distribution element 17 is combined with the positioning convex portion 18 or the positioning concave portion 19 of the upper distribution element 6 and the lower distribution element 8, and the positional relationship is uniquely determined. Therefore, a corresponding test is performed even when the central distribution element 17 is replaced. There is no change in the relative position of the tube 4, and there is no error in installing the test tube 4 in a position where the cleaning liquid is not supplied.

Further, the central distribution element 17 has a larger outer dimension than the upper distribution element 6 and the lower distribution element 8, and the groove 9
The portion in which is arranged is configured to be more convex outward than the other portions. As a result, the distance at which the test tube 4 and the cleaning liquid distribution element 5 interfere can be reduced, and as a result, the distance between the cleaning liquid distribution element 5 and the test tube 4 can be set small, and the cleaning liquid that has jumped out from the convex portion 15 can be removed. The flight direction is bent by the wind pressure of the rotation before entering the test tube 4
The number of defects that could not be entered was also reduced. Further, by configuring the central distribution element 17 with an elastic material such as silicon rubber, the test tube 4 is not damaged even if the test tube 4 is arranged at a distance in contact with the central distribution element 17, improving reliability. can do.

Further, by installing the porous filter 12 in the cleaning liquid introducing hole 7 of the upper distribution element 6, it becomes possible to remove the foreign matter in the cleaning liquid, clogging the groove 9 and blocking the flow path, and as a result, the cleaning liquid to the test tube 4 is removed. It is possible to prevent the supply amount from decreasing. Furthermore, as a result of manufacturing the upper distribution element 6 with a transparent member, even if the flow path is blocked by a foreign substance in the cleaning liquid, its position can be instantly specified, and it is possible to take an early action.

[0041]

As described above, in the present invention, the cleaning liquid distribution element is composed of the circular plate-shaped upper distribution element and the circular plate-shaped lower distribution element having the cleaning liquid introduction hole in the center, and the upper distribution element is formed. Either one or both of the element and the lower distribution element are provided with a plurality of radial grooves, or a circular flat plate-shaped upper distribution element having a cleaning liquid introduction hole in the center and a plurality of radially arranged grooves With the structure including the circular flat plate-shaped central distribution element and the circular flat plate-shaped lower distribution element, the flow path can be formed with high accuracy.

For this reason, it becomes possible to supply an equal amount of washing solution into a large number of test tubes, and as a result, good washing characteristics and high reliability, and moreover, a cell washing rotor capable of easily responding to an increase or decrease in the number of test tubes. Also, there is a remarkable effect that a cell washing centrifuge equipped with the same can be provided.

[Brief description of drawings]

FIG. 1 is a perspective view illustrating a structure and a component configuration of a cell washing rotor according to an embodiment of the present invention.

FIG. 2 is a perspective view illustrating a structure and a component configuration of a cleaning liquid distribution element according to an embodiment of the present invention.

FIG. 3 is a plan view of a cell washing rotor according to an exemplary embodiment of the present invention.

FIG. 4 is a plan view of a cell washing rotor according to an exemplary embodiment of the present invention.

FIG. 5 is a plan view of a lower distribution device according to an exemplary embodiment of the present invention.

FIG. 6 is a cross-sectional view of a cleaning liquid distribution element according to an exemplary embodiment of the present invention.

FIG. 7 is a perspective view illustrating a structure and a component configuration of a cleaning liquid distribution element according to another embodiment of the present invention.

FIG. 8 is a perspective view illustrating a structure and a component configuration of a cleaning liquid distribution element according to another embodiment of the present invention.

FIG. 9 is a cross-sectional view of a cleaning liquid distribution element according to another embodiment of the present invention.

FIG. 10 is a sectional view of a cell washing centrifuge according to an embodiment of the present invention.

[Explanation of symbols]

1 Cell washing rotor 2 rotor 3 Test tube holder 4 test tubes 5 Cleaning liquid distribution element 6 Upper distribution element 7 Cleaning liquid inlet 8 Lower distribution element 9 grooves 10 center line 11 groove end 12 Porous filter 13 Transparent member 14 grasping department 15 convex 16 Plug member 17 Central distribution element 18 Positioning convex part 19 Positioning recess 20 Cell washing centrifuge 21 motor 22 Drive shaft 23 pumps 25 nozzles 26 Anti-slip recess 27 Anti-slip convex part 28 Magnetic element 29 Anti-rotation protrusion 30 slots

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 33/48 G01N 1/28 J (72) Inventor Daijiro Shiraishi 1060 Takeda, Hitachinaka City, Ibaraki Hitachi, Ltd. Machine tool information technology F-term (reference) 2G045 BA13 BB10 BB14 CA02 CB01 JA07 2G052 AA33 AD26 CA03 CA08 DA02 DA12 DA22 EA03 EA14 EA17 FB02 FC06 FC11 FD13 HC36 JA01 JA05 JA07 JA08 JA01 JA15 JA16 JA23 4D057 AD01 A01 A01 AB01 AB01 A01

Claims (26)

[Claims] 1. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor to perform the test. In a cell washing rotor comprising a washing solution distribution element for supplying a washing solution into a large number of test tubes held by a tube holder, the washing solution distribution element has a washing solution introduction hole at a central portion, and the washing solution introduced from the introduction hole is provided. A cell washing rotor characterized in that it is distributed to the test tube and a removable filter is attached to the washing solution introducing hole. 2. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating with the rotor, In a cell cleaning rotor comprising a cleaning liquid distribution element for supplying a cleaning liquid into a large number of test tubes held by a tube holder, the cleaning liquid distribution element includes a circular flat plate-shaped upper distribution element and a circular flat plate shape having a cleaning liquid introduction hole in a central portion thereof. Cell washing, characterized in that it has a plurality of radial grooves in one or both of the upper distribution element and the lower distribution element, and that a detachable filter is attached to the cleaning solution introduction hole. Rotor. 3. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor, In a cell washing rotor comprising a washing solution distribution element for supplying a washing solution into a large number of test tubes held by a tube holder, the washing solution distribution element has a cone-shaped first portion having a washing solution introduction hole in a central portion and its periphery. An upper distribution element consisting of a circular flat plate-shaped second portion, a conical third portion facing the first portion, and a second portion facing the second portion. And a lower distribution element having a circular flat plate-shaped fourth portion, a flow path for the cleaning liquid is formed in the gap between the first portion and the third portion, and the second portion and the fourth portion are formed. One or both of the parts To form a distribution channel for cleaning liquid by grooves formed, cells washed rotor, characterized in that mounted a universal filter removably attached to the cleaning liquid introduction hole. 4. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor to perform the test. In a cell washing rotor comprising a washing solution distribution element for supplying a washing solution into a large number of test tubes held by a tube holder, the washing solution distribution element has a cone-shaped first portion having a washing solution introduction hole in a central portion and its periphery. An upper distribution element consisting of a circular plate-shaped second portion, a central distribution element arranged so as to face the second portion and having a hollow circular flat plate, and a conical shape. A lower distribution element disposed at a position facing the first portion and forming a fluid passage between the first portion and the lower portion of the second portion of the upper distribution element and the central portion. Distribution One or both of the upper surface of the child provided with a plurality of radial grooves, cell washing rotor, characterized in that mounted a universal filter removably attached to the cleaning liquid introduction hole. 5. The cell cleaning rotor according to claim 1, 2 or 3 or 4, wherein a part or all of the cleaning liquid distribution element is manufactured by molding resin or ceramics. 6. The center line of the plurality of grooves is radially arranged on a straight line passing through the center of the rotor, and the center lines of the grooves are the center of the test tube holder and the center of the test tube holder. A cell washing rotor characterized by not matching the straight line connecting the center of the rotor. 7. The rotor according to claim 1, 2 or 3 or 4, wherein the center lines of the plurality of grooves are the rotor viewed from above the straight line connecting the center of the test tube holder and the center of the rotor. A cell washing rotor characterized by advancing in the rotation direction of. 8. The cell cleaning rotor according to claim 1, 2 or 3 or 4, wherein the center lines of the plurality of grooves form a certain angle with a straight line or a curved line passing through the center of the rotor. . 9. The cell washing rotor according to claim 1, 2 or 3 or 4, wherein a groove end portion of the plurality of grooves on the rotor center side is formed of a smooth curved surface. 10. The method according to claim 1, 2 or 3 or 4,
The cell washing rotor, wherein the upper distribution element is partially or entirely formed of a transparent member. 11. The method according to claim 1, 2 or 3 or 4,
In the center of the upper distribution element has a cylindrical grasping portion,
A cell cleaning rotor characterized in that a plurality of non-slip concave portions or non-slip convex portions for vertical non-slip are formed on the outer periphery of the grasping portion. 12. The method according to claim 1, 2 or 3 or 4,
The cell washing rotor, wherein the washing liquid distribution element includes a plug member that engages with the upper distribution element and / or the central distribution element and the groove to block the flow of fluid. 13. The cell washing rotor according to claim 12, wherein the stopper member is made of an elastic material. 14. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor to perform the test. A cell cleaning rotor composed of a cleaning liquid distribution element for supplying a cleaning liquid into a large number of test tubes held by a tube holder, a rotor drive mechanism for rotating the rotor, and the cleaning liquid distribution element having a cleaning liquid introduction hole in the central portion. A cell washing centrifuge, characterized in that the washing liquid introduced from the introduction hole is distributed to the test tube, and a detachable filter is attached to the washing liquid introduction hole. 15. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor is rotated, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor, A cell cleaning rotor including a cleaning solution distribution element for supplying a cleaning solution into a large number of test tubes held by a tube holder, a rotor drive mechanism for rotating the rotor, and a cleaning solution supply for supplying the cleaning solution to the cell cleaning rotor. And a cleaning liquid distribution element,
It is composed of a circular plate-shaped upper distribution element and a circular plate-shaped lower distribution element having a cleaning liquid introduction hole in the central portion, and with a plurality of radial grooves in either or both of the upper distribution element and the lower distribution element, A cell washing centrifuge, wherein a detachable filter is attached to the washing liquid introducing hole. 16. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor, A cell cleaning rotor including a cleaning solution distribution element for supplying a cleaning solution into a large number of test tubes held by a tube holder, a rotor drive mechanism for rotating the rotor, and a cleaning solution supply for supplying the cleaning solution to the cell cleaning rotor. And a cleaning liquid distribution element,
An upper distribution element composed of a conical first portion having a cleaning liquid introduction hole in the central portion and a circular plate-shaped second portion around the conical portion, and a conical shape so as to face the first portion. The lower portion of the third portion and a fourth portion having a circular flat plate shape, which is arranged at a position facing the second portion, and includes the first portion and the third portion. A flow path for the cleaning liquid is formed in the gap, and a distribution channel for the cleaning liquid is formed by a groove provided in one or both of the second portion and the fourth portion, and a removable filter is attached to the cleaning liquid introduction hole. A cell washing centrifuge characterized by being attached. 17. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor, A cell cleaning rotor including a cleaning solution distribution element for supplying a cleaning solution into a large number of test tubes held by a tube holder, a rotor drive mechanism for rotating the rotor, and a cleaning solution supply for supplying the cleaning solution to the cell cleaning rotor. And a cleaning liquid distribution element,
An upper distribution element comprising a conical first portion having a cleaning liquid introduction hole in the central portion and a circular plate-shaped second portion around the conical first portion, and the upper distribution element is arranged so as to face the second portion. A central distribution element having a hollow circular flat plate in the central part, and a lower distribution part having a conical shape, which is arranged at a position facing the first part and forms a fluid passage between the first part and the first part. A plurality of radial grooves are formed on one or both of the lower surface of the second portion of the upper distribution element and the upper surface of the central distribution element, and a detachable filter is attached to the cleaning liquid introduction hole. A cell washing rotor characterized by: 18. The cell washing centrifuge according to claim 14, 15 or 16 or 17, wherein a part or all of the washing liquid distribution element is manufactured by molding a resin or ceramics. 19. The center line of the plurality of grooves is radially arranged on a straight line passing through the center of the rotor, and the center lines of the grooves are the center of the test tube holder and the center of the test tube holder. A cell washing centrifuge characterized by not matching the straight line connecting the rotor centers. 20. The rotor according to claim 14, 15 or 16 or 17, wherein the center lines of the plurality of grooves are the rotor viewed from above rather than the straight line connecting the center of the test tube holder and the center of the rotor. A cell washing centrifuge characterized by advancing in the rotation direction of. 21. The cell washing centrifuge according to claim 14, 15 or 16 or 17, wherein the center lines of the plurality of grooves form a straight line or a curved line and an angle with a straight line passing through the center of the rotor. Machine. 22. The cell washing centrifuge according to claim 14, 15 or 16 or 17, wherein a groove end portion of the plurality of grooves on the rotor center side is formed of a smooth curved surface. 23. The cell washing rotor according to claim 14, 15 or 16 or 17, wherein the upper distribution element is partially or entirely formed of a transparent member. 24. The gripping member according to claim 14, 15 or 16 or 17, wherein said upper distribution element has a cylindrical grasping portion in the center thereof, and a plurality of slips for preventing slippage in the longitudinal direction are provided on the outer periphery of the grasping portion. A cell washing centrifuge characterized in that a stop concave portion or a non-slip convex portion is formed. 25. The cleaning liquid distribution element according to claim 14, 15 or 16 or 17,
/ Or a cell washing centrifuge characterized by comprising a central distribution element and a stopper member that engages with the groove and blocks the flow of fluid 26. The cell washing centrifuge according to claim 25, wherein the stopper member is made of an elastic material.