JP2003334057A - Cell cleaning rotor and cleaning solution distribution element used therefor, and cell cleaning centrifugal machine equipped with the cleaning solution distribution element - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cell cleaning rotor which surely supplies equal amounts of a cleaning solution to a large number of test tubes and readily deals with the increase or decrease of the number of the test tubes and a cell cleaning centrifugal machine equipped with the cell cleaning rotor. <P>SOLUTION: In the cell cleaning rotor comprising a rotor, a plurality of test tube holders which are rotatably mounted on the rotor, moved in the outside direction by centrifugal force when the rotor is rotated and a cleaning solution distribution element for supplying the cleaning solution to a large number of the test tubes retained by the test tube holders, the cleaning solution distribution element is constituted of an upper distribution element composed of a first conical part having a cleaning solution inlet at the central part and a second circular planar part around the first part, a central part distribution element in which the central part arranged to face the second part comprises a hollow circular flat plate and a conical lower distribution element which is arranged at a position facing the first part and forms a fluid route between the first part and the lower distribution element. A plurality of radial channels are formed on one or both of the lower side of the second part of the upper distribution element and the upper side of the central distribution element to give the cell cleaning rotor having a structure that the channels are used as distributed fluid flow channels. The cell cleaning centrifugal machine is obtained by using the cell cleaning rotor. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cell washing centrifuge for washing living cells such as red blood cells by utilizing centrifugal force, and more particularly to a cell washing rotor used therein and a washing liquid distribution element for distributing a washing liquid.

[0002]

2. Description of the Related Art Conventionally, for antiglobulin test, cross-match test, irregular antibody screening, etc. at the time of blood transfusion test, erythrocytes are washed with a washing solution such as physiological saline to remove excess antibody in the suspension. A process is required, and various cell washing centrifuges are known for this purpose. For example, in Japanese Unexamined Patent Publication No. 50-22693, nozzles are installed radially from the outer periphery of the bottom surface of a conical container, and the cleaning liquid injected from the center of the cleaning liquid distribution element is equally divided by centrifugal force due to rotation. A cleaning liquid distribution element having a structure in which a cleaning liquid is supplied from a nozzle to a large number of test tubes held by a test tube holder is disclosed. Further, for example, Japanese Utility Model Laid-Open No. 2-81640 discloses a structure for supplying a cleaning liquid into a large number of test tubes held by a test tube holder from holes formed in a cleaning liquid distribution element.

[0003]

In order to perform a good blood transfusion test or the like by a centrifuge for the purpose of automating cell washing, the washing liquid distribution element supplies the same amount of washing liquid to a large number of test tubes. Is desirable. If there are variations in the amount of cleaning liquid supplied in multiple test tubes, for example,
In a test tube with a small amount of washing liquid supplied, a larger amount of foreign substances such as antibodies remain in the suspension than in other test tubes. On the contrary, in test tubes with a large supply of washing liquid, there are few residual foreign substances such as antibodies in the suspension, and this difference becomes the difference in the test results due to the reagent reaction performed in the subsequent process, and as a result, it can be used in the determination of blood transfusion tests. It causes a serious error. Also, if there is a test tube with a small amount of cleaning liquid, supplying the cleaning liquid to other test tubes according to the amount of cleaning liquid supplied to this test tube will cause an excessive amount of cleaning liquid to be supplied to the other test tubes, resulting in a large amount of cleaning liquid. Things spill over the tube and create the problem of losing valuable cell samples. Furthermore, if the number of washings is determined according to the amount of washing liquid, the washing process will take a long time. There are several possible causes for the non-uniform amount of cleaning liquid supplied to the test tube. The first cause is due to the non-uniformity of the flow path resistance of the cleaning liquid distribution element. For example, in the cleaning liquid distribution element shown in Japanese Utility Model Laid-Open No. 2-81640, which supplies a cleaning liquid into a large number of test tubes held by a test tube holder through holes that are radially drilled, holes are drilled or the like. Difficult except for machining. However, the machining of holes is
Errors easily occur in the shape of the entrance and exit of the hole and the roughness of the inner surface of the hole,
This causes non-uniform flow path resistance, resulting in variations in the amount of cleaning liquid supplied into a large number of test tubes. Further, Japanese Patent Laid-Open No. 50-22693 discloses a structure in which a metal pipe is embedded in a cleaning liquid distribution element and the cleaning liquid is supplied into a large number of test tubes using the metal pipe as a flow path. A processing error occurs in the processing shape of the pipe end surface and the pipe length, which causes uneven flow resistance as described above, resulting in variations in the amount of cleaning liquid supplied into many test tubes. The second cause of non-uniformity of the cleaning liquid is due to the leakage of the cleaning liquid. For example, if the distance between the cleaning liquid outlet of the cleaning liquid distribution element and the test tube opening is large, the cleaning liquid from the liquid distribution element may not completely enter the test tube due to an error in the drilling direction. This causes variation in the amount of the cleaning liquid supplied. Also, if the tip of the metal pipe is installed near the test tube so that the cleaning liquid completely enters the test tube, the test tube holder may not be tested due to variations in the length of the test tube or when the rotation is started. The tube comes into contact with the tip of the metal pipe, causing a problem that causes damage to the test tube. The third cause of non-uniformity of the cleaning liquid is due to foreign matter in the cleaning liquid. While the cleaning liquid sent out by the pump reaches the liquid distribution element, it traps dust and cotton dust in the air, and when these foreign substances become clogged in the holes and pipes of the cleaning liquid distribution element, the flow path is blocked, and as a result Reduce the wash solution supply to the corresponding test tube. In addition, when physiological saline or the like is used as the cleaning liquid as a cause of the foreign matter, solid substances such as sodium chloride deposited in the tank or the flow path may block the flow path and cause a decrease in the supply amount of the cleaning solution. In the above-mentioned cleaning liquid distribution element, it is not possible to see a foreign substance blocking such a flow path from the outside,
It was necessary to periodically suspend the cleaning process and check the supply amount of the cleaning liquid distributed to the test tube, which was a complicated work. Furthermore, in the above-described cleaning liquid distribution element, the cleaning liquid is always supplied by the number of holes or pipes regardless of the increase or decrease in the number of test tubes to be processed. Therefore, when the number of test tubes is small, the cleaning liquid is wasted. In view of the above-mentioned problems of the prior art, it is an object of the present invention to provide a cleaning liquid distribution element capable of supplying a uniform amount of cleaning liquid into a large number of test tubes. Another object of the present invention is to provide a cell washing rotor having good washing characteristics and high reliability by using the washing liquid distribution element as described above, and capable of easily responding to an increase or decrease in the number of test tubes, and a cell provided with the same. The purpose is to provide a washing centrifuge.

[0004]

In order to achieve the above object, the present invention provides a cleaning liquid distribution element for supplying a cleaning liquid into a test tube, and a conical first portion having a cleaning liquid introduction hole in a central portion thereof. An upper distribution element consisting of a circular plate-shaped second portion around it, a central distribution element arranged so as to face the second portion and having a hollow central circular plate, and a conical shape And a lower distribution element that is arranged at a position facing the above-mentioned first portion and forms a fluid passage between the first distribution portion and the lower surface of the second portion of the upper distribution element. Another feature is that a plurality of radial grooves are provided on one or both of the upper surfaces of the central distribution element, and the grooves serve as fluid distribution channels. With this configuration, the upper distribution element, the central distribution element, and the lower distribution element can be manufactured by molding with resin or ceramics, and an extremely accurate flow path can be formed, so that the flow path resistance is uniform. The remarkable effect that it can be realized is obtained. In particular, the effect is great when the space surrounded by the groove provided in one of the upper distribution element or the central distribution element and the plane of the other element is used as the flow path of the cleaning liquid. Another feature of the present invention is that the center lines of the plurality of grooves provided in one or both of the upper distribution element and the lower distribution element do not coincide with the straight line connecting the center of the test tube holder and the center of the rotor. Specifically, another feature is that the center line of the groove is provided at a position advanced in the rotation direction of the rotor with respect to the straight line connecting the center of the test tube holder and the center of the rotor. With this configuration, it is possible to prevent the cleaning liquid from leaking. Another feature of the present invention is that the center lines of a plurality of grooves provided in one or both of the upper distribution element and the lower distribution element form a certain angle with a straight line or a curved line passing through the center of the rotor. It is in. This is also effective in preventing leakage of the cleaning liquid. Still another feature of the present invention resides in that the groove end portions of the plurality of grooves on the rotor center side are formed by smooth curved surfaces. This configuration is effective in reducing the flow path resistance and making the amount of cleaning liquid uniform. Still another feature of the present invention is that a part or all of the upper distribution element is made of a transparent member. With this configuration, it is possible to easily determine whether or not the foreign matter is blocking the flow path. Still another feature of the present invention is to provide a cylindrical grasping portion at the center of the upper distribution element, and form a plurality of anti-slip concave portions or anti-slip convex portions for preventing slippage in the longitudinal direction on the outer periphery of the grasping portion. Especially. This is a convenient structure when the cell washing rotor is manually rotated in the reverse direction to accelerate the reagent reaction. Still another feature of the present invention resides in that, in the outermost periphery of the central distribution element, the portion in which the groove is arranged has a convex shape outward from the other portions. With this configuration, the cleaning liquid easily enters the test tube from the cleaning liquid distribution element, which is effective in preventing the cleaning liquid from leaking.

Still another feature of the present invention is to provide a concave portion or a convex portion for engaging with a positioning convex portion or a concave portion of the upper distribution element and the lower distribution element on the upper surface and the lower surface of the central distribution element. Especially. With this structure, the positional relationship among the distribution elements can be uniquely determined. Still another feature of the present invention is that a part or all of the central distribution element is made of an elastic material. This makes it possible to prevent damage to the test tube.

Still another feature of the present invention is that the outer dimensions of the central distribution element are larger than the outer dimensions of the upper distribution element and the lower distribution element. This is effective in preventing the leakage of the cleaning liquid. Still another feature of the present invention is that the upper distribution element, the central distribution element, and the stopper member that engages with the groove to block the flow of fluid are provided, and the stopper member is made of an elastic material. With this configuration, it is possible to easily cope with an increase or decrease in the number of test tubes.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view illustrating a structure and a component configuration of a cell washing rotor showing an embodiment of the present invention. In this embodiment, the cell washing rotor 1 is composed of a rotor 2 and a washing liquid distribution element 5, and the disk-shaped rotor 2 has 24 outer circumferences.
24 square tube holes 3 are placed in this hole.
Is installed. Test tube holder 3 with test tube 4 inserted
When the rotor 2 rotates, it can freely move in the horizontal direction by the centrifugal force about the mounting portion. In this embodiment, the rotor 2
The test tube holder 3 is manufactured by pressing a stainless plate, but a resin molded product can also be manufactured. A cleaning liquid distribution element 5 for supplying a cleaning liquid to the test tube 4 is mounted on the rotor 2. The cleaning liquid distribution element 5 rotates with the rotor 2 because the lower convex portion thereof engages with the elongated hole of the rotor 2. Further, even if the cleaning liquid distribution element 5 is attached to and detached from the rotor, the positional relationship between the cleaning liquid distribution element 5 and the rotor 2 and the test tube holder 3 does not change. FIG. 2 is a perspective view for explaining the structure and component configuration of the cleaning liquid distribution element showing one embodiment of the present invention. In the present embodiment, the cleaning liquid distribution element 5 is composed of an upper distribution element 6 and a lower distribution element 8, and the lower distribution element 8 is formed with 24 grooves 9 radially corresponding to the number of the test tubes 4. Upper distribution element 6 and lower distribution element 8
By arranging in combination, the space formed by the groove 9 of the lower distribution element 8 and the flat portion of the upper distribution element 6 becomes the flow path of the cleaning liquid. According to this embodiment, by making the cleaning liquid flow path surrounded by the groove and the plane, the upper distribution element 6 and the lower distribution element 8 can be manufactured only by resin molding. By resin-molding the upper distribution element 6 and the lower distribution element 8 using a high-precision mold, the shape of the groove 9 of the lower distribution element 8 becomes uniform with high accuracy in the same cleaning liquid distribution element 5, and the performance is improved. It has become possible to mass-produce the cleaning liquid distribution element 5 having a uniform flow resistance at any time. In the above embodiment, the groove may be provided in one or both of the upper distribution element 6 and the lower distribution element 8, or these may be made of ceramics or the like instead of resin. Considering the ease of fabrication and the uniformity of flow path resistance, it is preferable to provide a groove in the lower distribution element 8 and use a space formed between the lower distribution element 8 and the plane of the upper distribution element 6 as a flow path as in the above embodiment. The best. Further, in FIG. 2, a cylindrical grasping portion 14 is provided at the center of the upper distribution element 6. A plurality of anti-slip concave portions 26 and anti-slip convex portions 27 are provided on the outer periphery of the grasping portion 14 in the vertical direction. This is convenient when the rotor 1 is manually manually rotated in small increments after the cell washing step to satisfactorily mix the red blood cells and the reagent in the test tube 4 to promote the reaction. FIG. 10 is a vertical cross-sectional view showing the overall configuration of the cell washing centrifuge 20 according to the present invention. The function of the cleaning liquid distribution element 5 will be described in more detail with reference to FIG. Figure 10
At, the cell washing rotor 1 is attached to the drive shaft 22 of the motor 21. A drive voltage is applied to the motor 21 from a drive circuit (not shown), and the drive shaft 22 is rotated. The cell washing rotor 1 is rotationally driven by a drive shaft 22, and the test tube holder 3 and the test tube 4 containing an appropriate amount of living cells such as red blood cells in advance are horizontally swung by centrifugal force.
At this time, the pump 23 is operated to feed the washing liquid from the external washing liquid tank to the nozzle 25 located at the upper part of the cell washing centrifuge 20, and the washing liquid is jetted downward from the nozzle 25 to the center of the rotating cell washing rotor 1. It enters into the cleaning liquid distribution element 5 through the cleaning liquid introduction hole 7. The cleaning liquid that has entered the cleaning liquid distribution element 5 moves to the outer periphery by centrifugal force, and is composed of the groove 9 of the lower distribution element 8 and the flat portion of the upper distribution element 2.
The flow is divided into four flow paths and flows in, and vigorously jumps out from the outer periphery of the cleaning liquid distribution element 5. The washed-out washing solution hits the inner wall of the test tube 4 located outside the washing solution distribution element 5, travels along the wall surface, and the living cells at the bottom of the test tube 4 are suspended to create a suspended state. When an appropriate amount of cleaning liquid enters test tube 4, pump 2
3 is stopped, and the cleaning liquid injection process is completed. Continuing,
The rotation is continued until the floating living cells gather at the bottom of the test tube 4. After that, the rotation is stopped and the test tube holder 3 is returned to the vertical position. Next, in this embodiment, the motor 21 is rotated at a low speed while the magnetic element 28 serving as a means for restraining the horizontal movement of the test tube holder 3 functions. Since the test tube holder 3 is made of a magnetic material such as SUS430 material, the test tube holder 3 is attracted by the magnetic force of the magnetic element 28 to rotate in a vertical state or while holding the opening of the test tube 4 slightly tilted outward. The cleaning liquid in the test tube 4 is discharged to the outside by centrifugal force. Living cells at the bottom of the test tube 4 remain as they are, and only the washing solution is lost. By repeating such steps, the cell washing centrifuge 20 separates and removes foreign substances such as antibodies from the living cells in the test tube 4. Since the washing process is carried out by such a process, it is desirable for the performance of the cell washing centrifuge 20 that the washing liquid supplied into a large number of test tubes is equal in volume. According to the embodiment shown in FIG. 2, since the upper distribution element 6 and the lower distribution element 7 of the cleaning liquid distribution element 5 are made of high-precision resin molded products, it is possible to compare them with the perforated holes and the flow path of the metal pipe. The shape of the groove 9 of the lower distribution element 8 can be made uniform with high precision. Therefore, it is possible to mass-produce the cleaning liquid distribution element 5 with good reproducibility and capable of distributing an equal amount of cleaning liquid with a uniform flow resistance as compared with the perforated hole or the flow path of the metal pipe. Becomes After the cell washing step of the cell washing centrifuge 20, for example, after washing red blood cells, a reagent such as antiglobulin serum is dropped onto the test tube 4, and the cell washing rotor 1 is manually rotated in forward and reverse directions to make the test tube 4 It is necessary to satisfactorily mix the red blood cells and the reagent in the inside to promote the reaction, but in the embodiment of FIG. 2, the grasping portion 14 is provided at the center of the upper distribution element 6, and the slip preventing recess 26 is further provided on the outer periphery. By providing the non-slip convex portion 27, it becomes easy to manually rotate in forward and reverse directions in small steps, and there is an effect that a reliable reagent reaction can be promoted. FIG. 3 is a plan view of a cell washing rotor according to an exemplary embodiment of the present invention. When the rotation speed of the motor 21 is increased in order to shorten the processing time of the cell washing centrifuge 20, the flight direction is bent by the wind pressure of the rotation until the washing solution that has jumped from the outer periphery of the washing solution distribution element 5 enters the test tube 4, and is completely To test tube 4
Occasionally it will not fit inside. In the embodiment of FIG. 3, the groove 9
The groove 9 is provided at a position where the center line 10 of the is advanced from the straight line connecting the center of the test tube holder 3 and the rotation center of the rotor 2 in the rotation direction of the rotor 2. By doing so, the bending of the cleaning liquid in the flight direction can be corrected, and the cleaning liquid can be reliably injected into the test tube 4 as shown in the figure. The angle of advance is the rotation speed of the rotor 2 and the cleaning liquid distribution element 5.
Although it depends on the distance between the test tube 4 and the test tube 4, it is preferably 0.5 to 5 degrees, more preferably 1 to 3 degrees. FIG. 4 is a plan view of a cell washing rotor according to an embodiment of the present invention. The center line 10 of the groove 9 is a straight line or a curved line and is installed at an angle with a straight line passing through the center of the rotor. Also in this example, the bending of the cleaning liquid in the flight direction can be corrected by advancing the angle formed by the center line 10 of the groove and the straight line passing through the center of the rotor in the rotation direction of the rotor 2. FIG. 5 is a plan view of the lower distribution element 8 according to an embodiment of the present invention. In this embodiment, the groove end portion 11 of the groove 9 on the center side of the rotor 2 is formed by a smooth curved surface. With such a shape, the cleaning liquid is introduced into the cleaning liquid introducing hole 7
Therefore, it is possible to suppress variations in flow path resistance when entering the cleaning liquid distribution element 5 and flowing into the plurality of grooves 9. Particularly, when the adjacent groove end portions 11 are formed by semicircular arcs as in this embodiment, it is effective in reducing the variation in the flow path resistance. Further, in FIG. 5 and FIG. 2, the upper distribution element 6 and the lower distribution element 8 have grooves 9 at the outermost periphery.
The convex portion 15 is provided so that the portion where is arranged is convex toward the outside as compared with other portions. With such a shape, the distance at which the test tube 4 and the cleaning liquid distribution element 5 interfere can be reduced. As a result, the distance between the cleaning liquid distribution element 5 and the test tube 4 can be set small, and the direction of flight is bent by the wind pressure of the rotation until the cleaning liquid jumping out from the convex portion 15 enters the test tube 4, and inside the test tube 4. The trouble of not entering has also decreased. Further, it is possible to eliminate a useless portion on the outer periphery of the cleaning liquid distribution element 5, and as a result, the weight of the cleaning liquid distribution element 5 is reduced, and the load on the motor 21 can be reduced. FIG. 6 is a sectional view of the cleaning liquid distribution element 5 according to an embodiment of the present invention. A porous filter 12 that is detachable from above in a cleaning liquid introduction hole 7 located in the center of the upper distribution element 6.
I put on. With such a configuration, foreign matter in the cleaning liquid can be removed before entering the cleaning liquid distribution element 5, clogging the groove 9 and blocking the flow path, and as a result, the test tube 4
It was possible to prevent a decrease in the amount of cleaning liquid supplied to the. In this embodiment, the porous filter 12 is made of a sintered polypropylene resin product having a filtration accuracy of 50 μm, and after being used for a long period of time, the filter is clogged and can be replaced with a new one before the flow rate decreases. In addition to this, there is no practical problem even if the porous filter 12 is manufactured from a screen mesh made of metal such as stainless steel. Further, in FIG. 6, the upper distribution element 6 is entirely made of a transparent member. As a result, the groove 9 of the lower distribution element 8 can be visually observed from above, and even if the flow path is blocked by a foreign substance in the cleaning liquid, its position can be instantly specified and an early countermeasure can be taken. If the groove 9 is observable, the same effect can be obtained even if only a part of the upper distribution element 6 is made of a transparent member. FIG. 7 is a perspective view illustrating a structure and a component structure of a cleaning liquid distribution element according to another embodiment of the present invention. The above-mentioned cell washing rotor 1 holds 24 test tube holders 3, but it is not always necessary to carry out 2 washing steps per washing process.
Washing of the four test tubes 4 is not performed, and in some cases 1
Sometimes two test tubes 4 are processed. In this case, since the cleaning liquid is supplied and injected also to the portion without the test tube 4, the cleaning liquid is wasted. In the embodiment shown in FIG. 7, the cleaning liquid distribution element 5 includes an upper distribution element 6, a lower distribution element 8 and a plug member 16.
The stopper member 16 is structured so as to be held between the upper distribution element 6 and the lower distribution element 8 and to be in close contact with and engaged with the groove end portion 11. As a result, it was possible to completely stop the flow of the cleaning liquid in the groove 9 with which the plug member 16 was engaged. Therefore 2
When performing the washing process using 12 test tubes 4 in the cell washing rotor 1 having the four test tube holders 3, the plug members 16 are closely attached to the groove end portions 12 at 12 places where the test tubes 4 are not installed. By combining them, useless supply of the cleaning liquid portion can be stopped. Of course, it is desirable to install the plug member 16 at a position symmetrical with respect to the center of the cell washing rotor 1. Furthermore, since the upper distribution element 6 is partially or wholly formed of a transparent member, the position of the plug member 16 can be confirmed from above, and the test tube 4 can be reliably installed at the cleaning liquid supply point. Further, since the plug member 16 is made of an elastic material such as silicon rubber, the groove end portion 11 is further improved.
It is possible to improve the adhesiveness with and reliably stop the flow of the cleaning liquid. FIG. 8 is a perspective view illustrating a structure and a component configuration of a cleaning liquid distribution element according to another embodiment of the present invention. In the embodiment shown in FIG. 8, the cleaning liquid distribution element 5 is the central distribution element 1 in which the upper distribution element 6, the lower distribution element 8 and the groove 9 are formed.
It consists of 7. With such a configuration, the cleaning liquid distribution element 5 has the central portion distribution element 1 having different numbers of grooves.
By sequentially replacing 7, it is possible to easily cope with an increase or decrease in the number of test tube treatments, and as a result, it is possible to surely eliminate the waste of the cleaning liquid section. Further, FIG. 9 is a cross-sectional view of the cleaning liquid distribution element 5 of this configuration. The central distribution element 17 is combined with the positioning convex portion 18 or the positioning concave portion 19 of the upper distribution element 6 and the lower distribution element 8, and the positional relationship is uniquely determined. Therefore, a corresponding test is performed even when the central distribution element 17 is replaced. There is no change in the relative position of the tube 4, and there is no error in installing the test tube 4 in a position where the cleaning liquid is not supplied. Further, the central distribution element 17 has a larger outer dimension than the upper distribution element 6 and the lower distribution element 8, and is configured such that the portion in which the groove 9 is arranged is more convex outward than the other portions. .
As a result, the distance at which the test tube 4 and the cleaning liquid distribution element 5 interfere can be reduced, and as a result, the cleaning liquid distribution element 5
The distance between the test tubes 4 can be set small, and the flight direction of the cleaning liquid jumping out from the convex portion 15 is bent by the wind pressure of the rotation before entering the test tube 4, and the problem of not entering the test tube 4 is reduced. Further, by configuring the central distribution element 17 with an elastic material such as silicon rubber, the test tube 4 is not damaged even if the test tube 4 is arranged at a distance in contact with the central distribution element 17, improving reliability. can do. Further, by mounting the porous filter 12 in the cleaning liquid introduction hole 7 of the upper distribution element 6, it becomes possible to remove foreign matters in the cleaning liquid, clogging the groove 9 and blocking the flow path, and as a result, the amount of the cleaning liquid supplied to the test tube 4 is increased. It can be prevented from decreasing. Furthermore, as a result of manufacturing the upper distribution element 6 with a transparent member, even if the flow path is blocked by a foreign substance in the cleaning liquid, its position can be instantly specified, and it is possible to take an early action.

[0008]

As described above, in the present invention, the cleaning liquid distribution element is composed of the circular plate-shaped upper distribution element and the circular plate-shaped lower distribution element having the cleaning liquid introduction hole in the center, and the upper distribution element is formed. Either one or both of the element and the lower distribution element are provided with a plurality of radial grooves, or a circular flat plate-shaped upper distribution element having a cleaning liquid introduction hole in the center and a plurality of radially arranged grooves With the structure including the circular flat plate-shaped central distribution element and the circular flat plate-shaped lower distribution element, the flow path can be formed with high accuracy. Therefore, it becomes possible to supply an equal amount of cleaning liquid into a large number of test tubes, and as a result,
There is a remarkable effect that it is possible to provide a cell washing rotor that has good washing characteristics and high reliability, and that can easily cope with an increase or decrease in the number of test tubes, and a cell washing centrifuge equipped with the same.

[Brief description of drawings]

FIG. 1 is a perspective view illustrating a structure and a component configuration of a cell washing rotor according to an embodiment of the present invention.

FIG. 2 is a perspective view illustrating a structure and a component configuration of a cleaning liquid distribution element according to an embodiment of the present invention.

FIG. 3 is a plan view of a cell washing rotor according to an exemplary embodiment of the present invention.

FIG. 4 is a plan view of a cell washing rotor according to an exemplary embodiment of the present invention.

FIG. 5 is a plan view of a lower distribution device according to an exemplary embodiment of the present invention.

FIG. 6 is a cross-sectional view of a cleaning liquid distribution element according to an exemplary embodiment of the present invention.

FIG. 7 is a perspective view illustrating a structure and a component configuration of a cleaning liquid distribution element according to another embodiment of the present invention.

FIG. 8 is a perspective view illustrating a structure and a component configuration of a cleaning liquid distribution element according to another embodiment of the present invention.

FIG. 9 is a cross-sectional view of a cleaning liquid distribution element according to another embodiment of the present invention.

FIG. 10 is a sectional view of a cell washing centrifuge according to an embodiment of the present invention.

[Explanation of symbols]

1 Cell washing rotor 2 rotor 3 Test tube holder 4 test tubes Cleaning liquid distribution element Upper distribution element Cleaning liquid inlet Lower distribution element 9 grooves 10 center line 11 groove end 12 Porous filter 13 Transparent member 14 grasping department 15 convex 16 Plug member 17 Central distribution element 18 Positioning convex part 19 Positioning recess 20 Cell washing centrifuge 21 motor 22 Drive shaft 23 pumps 25 nozzles 26 Anti-slip recess 27 Anti-slip convex part 28 Magnetic element 29 Anti-rotation protrusion 30 slots

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) B08B 11/00 B08B 11/00 D 4G068 G01N 33/48 G01N 33/48 C (72) Inventor Daijiro Shiraishi Ibaraki Ibaraki 1060 Takeda, Hitachinaka, Japan F-Term in Hitachi Koki Information Technology Co., Ltd. (reference) 2G045 AA01 BB10 CA25 3B116 AA23 AA46 AB05 AB44 3B201 AA23 AA46 AB05 AB44 BB01 BB62 BB92 4B029 AA11 AA27 BB11 CC01 AC11A01A01A11A01A01A11A01 4G068 AA03 AB15 AC10 AD45

Claims (34)

[Claims] 1. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor to perform the test. In a cell washing rotor comprising a washing solution distribution element for supplying a washing solution into a large number of test tubes held by a tube holder, the washing solution distribution element has a cone-shaped first portion having a washing solution introduction hole in a central portion and its periphery. An upper distribution element consisting of a circular plate-shaped second portion, a central distribution element arranged so as to face the second portion and having a hollow circular flat plate, and a conical shape. A lower distribution element disposed at a position facing the first portion and forming a fluid passage between the first portion and the lower portion of the second portion of the upper distribution element and the central portion. Distribution Cells washed rotor, characterized in that a one or both radially of the plurality of grooves of the upper surface of the child. 2. The cell cleaning rotor according to claim 1, wherein the upper distribution element, the central distribution element and the lower distribution element are manufactured by molding resin or ceramics. 3. The cell washing method according to claim 1, wherein a space provided by one of the upper distribution element and the central distribution element and a space surrounded by a plane of the other element serves as a flow path of a washing solution. Rotor. 4. The groove according to claim 1, wherein center lines of a plurality of grooves provided in one or both of the upper distribution element and the central distribution element are radially arranged on a straight line passing through the center of the rotor, The center line of the cell washing rotor does not coincide with the straight line connecting the center of the test tube holder and the center of the rotor. 5. The center line of the plurality of grooves according to claim 4, wherein the center line of the plurality of grooves is advanced in the rotational direction of the rotor as viewed from above the rotor, as compared with a straight line connecting the center of the test tube holder and the center of the rotor. A cell washing rotor characterized in that 6. The center line of a plurality of grooves provided in one or both of the upper distribution element and the central distribution element according to claim 1, wherein a straight line or a curved line forms an angle with a straight line passing through the rotor center. A cell washing rotor characterized by being formed. 7. The cell washing rotor according to claim 1, wherein the groove ends of the plurality of grooves on the rotor center side are formed of smooth curved surfaces. 8. The upper distribution element according to claim 1, wherein:
A cell washing rotor, characterized in that a part or all of it is made of a transparent member. 9. The cylindrical grip portion according to claim 1, wherein the grip portion has a cylindrical shape in the center thereof, and the grip portion has a plurality of anti-slip recesses or anti-slip projections for preventing slip in the longitudinal direction. A cell washing rotor characterized in that a portion is formed. 10. The upper distribution element and the central distribution element according to claim 1, wherein the outermost periphery of the distribution element has a convex portion in which a portion where the groove is arranged is convex outward from other portions. A cell washing rotor comprising: 11. A washing liquid distribution element in a cell washing rotor, the upper distribution comprising a conical first portion having a washing liquid introduction hole in a central portion and a circular plate-shaped second portion around the first portion. An element, a central part distribution element having a central circular portion which is a hollow circular flat plate arranged so as to face the second portion, and has a conical shape, and is arranged at a position facing the first portion, A lower distribution element that forms a fluid passage between the first distribution element and a plurality of radial grooves formed on one of the lower surface of the second portion of the upper distribution element and the upper surface of the central distribution element, A cleaning liquid distribution element, wherein a space surrounded by the groove and the plane is a fluid distribution channel. 12. The cleaning liquid distributing element according to claim 11, wherein the upper distributing element, the central distributing element and the lower distributing element are manufactured by molding resin or ceramics. 13. A plurality of grooves provided in one of the upper distribution element or the central distribution element are arranged radially, and a center line of the groove is a straight line connecting a center of the test tube holder and a center of the rotor. Cleaning liquid distribution element characterized by not matching with. 14. The center line of the plurality of grooves according to claim 13, wherein the center line of the plurality of grooves is moved forward in the rotational direction of the rotor when viewed from above the rotor with respect to a straight line connecting the center of the test tube holder and the center of the rotor. A cleaning liquid distribution element characterized in that 15. The center line of a plurality of grooves provided in one of the upper distribution element and the central distribution element according to claim 11, forming a certain angle with a straight line or a curved line passing through the center of the rotor. A cleaning liquid distribution element characterized in that 16. The cleaning liquid distribution element according to claim 11, wherein the ends of the plurality of grooves on the rotor center side are formed by smooth curved surfaces. 17. The cleaning liquid distribution element according to claim 11, wherein a part or all of the upper distribution element is made of a transparent member. 18. The gripping portion having a cylindrical shape at the center of the upper distribution element, and a plurality of non-slip concave portions or non-slip convex portions for vertical non-slip on the outer periphery of the gripping portion. A cleaning liquid distribution element characterized in that a portion is formed. 19. The upper distribution element and the central distribution element according to claim 11, wherein a portion where the groove is arranged has a convex portion which is convex outwardly than the other portion on the outermost periphery thereof. A cleaning liquid distribution element characterized by the above. 20. The central part distribution element according to claim 11, wherein the upper surface and the lower surface of the central part distribution element have concave parts or convex parts for engaging with positioning convex parts or concave parts of the upper distribution element and the lower distribution element. Characteristic cleaning liquid distribution element. 21. The cleaning liquid distribution element according to claim 11, wherein a part or all of the central distribution element is made of an elastic material. 22. The cleaning liquid distribution element according to claim 11, wherein an outer dimension of the central distribution element is larger than outer dimensions of the upper distribution element and the lower distribution element. 23. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor to perform the test. In a cell washing rotor comprising a washing solution distribution element for supplying a washing solution into a large number of test tubes held by a tube holder, the washing solution distribution element has a cone-shaped first portion having a washing solution introduction hole in a central portion and its periphery. An upper distribution element having a circular plate-shaped second portion, and a central portion distribution element arranged so as to face the second portion and having a hollow central circular plate.
A lower distribution element having a conical shape and arranged at a position facing the first portion to form a fluid passage between the first portion and the lower surface of the second portion of the upper distribution element; And a plurality of radial grooves are provided on one of the upper surfaces of the central distribution element, and a space surrounded by the grooves and the plane is used as a fluid distribution channel, and the upper distribution element, the central distribution element and the groove are formed. A cell cleaning rotor, comprising a stopper member that engages with and blocks a flow of fluid. 24. The cell cleaning rotor according to claim 23, wherein the stopper member is made of an elastic material. 25. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor, A cell cleaning rotor consisting of a cleaning solution distribution element for supplying a cleaning solution into a large number of test tubes held by a tube holder, a rotor drive mechanism for rotating the rotor in the previous period, and a cleaning solution for supplying the cleaning solution to the cell cleaning rotor. And a supply mechanism, wherein the cleaning liquid distribution element has a conical shape having a cleaning liquid introduction hole in a central portion thereof.
And a central part distributing element composed of a circular flat plate having a hollow central part and arranged so as to face the second part. , A conical shape, which comprises a lower distribution element which is arranged at a position facing the first part and which forms a fluid passageway between the first part and the second part of the upper distribution element. A cell washing centrifuge characterized in that a plurality of radial grooves are provided on one of a lower surface and an upper surface of the central distribution element, and a space surrounded by the grooves and a plane serves as a fluid distribution channel. 26. The cell washing centrifuge according to claim 25, wherein the upper distributing element, the central distributing element and the lower distributing element are manufactured by molding resin or ceramics. 27. The center line of a plurality of grooves provided in one of the upper distribution element or the central distribution element is arranged radially on a straight line passing through the center of the rotor, and the center of the groove is formed. The line does not coincide with a straight line connecting the center of the test tube holder and the center of the rotor, the cell washing centrifuge. 28. The center lines of the plurality of grooves are advanced in the rotation direction of the rotor as viewed from above the rotor, as compared with a straight line connecting the center of the test tube holder and the center of the rotor. A cell washing centrifuge characterized in that 29. The center line of a plurality of grooves provided in one of the upper distribution element and the central distribution element is a straight line or a curved line and forms an angle with a straight line passing through the center of the rotor. A cell washing centrifuge characterized in that 30. The cell washing centrifuge according to claim 25, wherein a groove end portion of the plurality of grooves on the rotor center side is formed of a smooth curved surface. 31. The cell washing centrifuge according to claim 25, wherein a part or all of the upper distribution element is made of a transparent member. 32. The gripping portion having a cylindrical shape is provided in the center of the upper distribution element, and a plurality of anti-slip concave portions or anti-slip projections for longitudinal non-slip are provided on the outer periphery of the grasping portion. A cell washing centrifuge characterized in that a portion is formed. 33. The central distributing element according to claim 25, wherein a portion in which the groove is arranged has a convex portion which is convex outward from the other portion on the outermost periphery thereof. Cell washing centrifuge. 34. A rotor, a plurality of test tube holders rotatably mounted on the rotor and capable of moving outward due to centrifugal force when the rotor rotates, and a plurality of test tube holders mounted on the rotor and rotating together with the rotor, A rotation mechanism including a fluid distribution element for supplying fluid into a large number of test tubes held by a tube holder, a rotor drive mechanism for rotating the rotor, and a fluid supply for supplying fluid to the fluid distribution element. A mechanism, wherein the fluid distribution element includes an upper distribution element including a conical first portion having a fluid introduction hole in a central portion and a circular flat plate-shaped second portion around the first portion; A central part distribution element having a circular flat plate whose central part is arranged so as to face the second part, and has a conical shape;
The lower distribution element is disposed at a position facing the first portion and forms a fluid passage between the first distribution element and the first portion, and the lower surface of the second portion of the upper distribution element and the central distribution element. A centrifuge characterized in that a plurality of radial grooves are provided on one of the upper surfaces of the elements, and a space surrounded by the grooves and a plane serves as a fluid distribution channel.