JP2003315351A - Infinitesimal blood automatic analytical device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a simple and low-cost infinitesimal blood automatic analytical device capable of performing an inspection similar to a conventional automatic analytical device for an infant or a serious case with an infinitesimal specimen and a small quantity of reagent. <P>SOLUTION: This infinitesimal blood automatic analytical device is constituted as follows: a prescribed quantity of reagent corresponding to a measurement item is sucked by a reagent nozzle of a reagent dispensation device; the reagent adhering to the outer surface of the reagent nozzle is wiped out by an adhering reagent wiping part; the reagent nozzle is transferred to the upper side of a reaction vessel and the sucked reagent is dispensed; a specimen necessary for the measurement item is sucked by a specimen nozzle of a specimen dispensation device from a specimen container into the reaction vessel wherein the reagent is dispensed; the specimen adhering to the outer surface of the specimen nozzle is wiped out by an adhering liquid wiping part; the specimen nozzle is transferred to the upper side of the reaction vessel; the specimen quantity corresponding to a reagent item in the reaction vessel is discharged, dispensed and stirred by a stirring rod in a stirring device; and optical measurement of the reaction specimen heated and reacted in the constant temperature state is performed with a prescribed wavelength. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an ultratrace blood automatic analyzer capable of performing an analytical test with a very small amount of blood and a very small amount of reagent, and in particular, the amount of specimen and reagent used is greatly reduced. The present invention relates to an automatic micro blood analyzer that enables blood tests to be performed on pets such as birds that can only collect a very small amount of blood.

[0002]

2. Description of the Related Art GOT, GPT in blood and urine,
Automatic analyzers that analyze components such as ALP and TP are often used in medical sites such as hospitals, and the test results are heavily used as treatment data. Currently, they are required for general biochemical automatic analyzers. Specimen volume is approximately 3 per test
It is set to ~ 30 μl, and in the immunochemical automatic analyzer,
It is approximately 10 to 100 μl.

Therefore, since the conventional dispensing technology has a limit of 3 μl (guaranteeing that the fluctuation count CV is 2% or less), the minimum amount of the sample that can be dispensed, 3 μl, is used as the minimum standard, and the amount of the sample and the reagent for each test item are determined. The quantity has been determined.

However, in such a conventional dispensing method, the minimum amount of sample that can be dispensed is 3 μl, so that
The sample volume of the item with the highest sensitivity among the test items to be analyzed at the same time is set to 3 μl, and the final reaction solution volume is determined from the reagent volume matching this sample volume, and the relationship with this final reaction solution volume is considered. Thus, the sample amount and reagent amount of other items are determined. For this reason, it is the current situation that conventional analyzers cannot adequately test infants and critically ill patients whose blood sampling volume is small. Therefore, there is a strong demand for the appearance of an analyzer capable of sufficiently testing infants and critically ill patients by significantly reducing the amount of specimen per test.

When using expensive reagents used in immunological tests or gene analysis, reducing the final reaction solution lowers the analysis cost, but 3 μl
Since the item is bound by the final volume, it is not possible to reduce the analysis cost of tests using expensive reagents of this kind.
As described above, at present, there is a strong demand from the inspection side from the viewpoint of both enabling the examination of infants and critically ill patients and reducing the examination cost.

On the other hand, in animal (dog, cat, bird, etc.) hospitals, blood tests are currently conducted using an automatic analyzer for humans in order to understand the condition of pets. Currently, there are about 10,000 animal hospitals in Japan, and about 90% of them have a simple automatic analyzer.

However, the amount of blood that can be collected from sick pets such as dogs and cats is generally small, and the amount of blood collected from sick birds is extremely small, ie, several hundreds of μl, which makes it impossible to carry out tests. For this reason, conventionally, the present condition is that the examination is limited to the minimum necessary items, and there is a problem that it is very difficult to grasp an accurate medical condition.

In order to solve such a problem, in the case of an automatic analyzer having a minimum dispensed sample amount of 1/10 of the conventional one,
Even if the amount of serum is 30μ, about 20 items can be tested, and the number of pets is expected to increase in the future. Therefore, the number of veterinary hospitals is expected to increase by about 10% annually, especially in veterinary hospitals in Europe and America. Since it is common to provide pets with the same services as humans, there is a great demand for pets under the present circumstances.

The present invention was devised in view of the present situation, and its object is to use a very small amount of a sample and a small amount of a reagent, as well as a test similar to a conventional automatic analyzer. It is ideal for emergency tests and satellite tests in hospitals, and can be used for blood tests of animals that require a small amount of blood such as pets. An attempt is made to provide an automatic micro blood analyzer.

[0010]

In order to achieve the above object, in the present invention, an ultratrace blood automatic analyzer is provided.
As described in claim 1, after a predetermined amount of reagent corresponding to the measurement item is sucked by the reagent nozzle of the reagent dispensing apparatus, the reagent adhering to the outer surface of the reagent nozzle is wiped by the adhering reagent wiping unit, After this, the reagent nozzle is transferred onto the reaction container to dispense a suction reagent, and to the reaction container in which this reagent has been dispensed,
After sucking the sample required for the measurement item from the sample container with the sample nozzle of the sample dispensing apparatus, the sample adhered to the outer surface of the sample nozzle is wiped by the attached liquid wiping unit, and then the sample nozzle is moved to the reaction container. After transferring to the upper part, discharging and dispensing the sample amount corresponding to the reagent item in the reaction container, stirring by the stirring rod of the stirrer, the reaction sample heated and reacted in a constant temperature state is optically reflected at a predetermined wavelength. It is characterized by being configured to measure.

Further, the adhering reagent wiping portion, the adhering liquid wiping portion, and the stirring rod wiping portion have the following features.
By disposing a liquid-absorbing tape, and the liquid-absorbing tape is configured to be wound up in a fixed amount after absorbing the liquid, it is possible to accurately perform quantification of a very small amount of sample or reagent, and to use a stirring rod. By preventing the reaction liquid and the cleaning liquid from being attached, it is possible to perform highly reliable and accurate measurement and to significantly reduce the cost.

Further, in the ultratrace blood automatic analyzer according to the present invention, as described in claim 3, the flow passage connecting the nozzles and the pump for communication is filled with washing water in a normal state. In addition, when the sample or the reagent is sucked, the pump is suctioned so that air bubbles are present between the sample or the reagent and the washing water so that they are not mixed with each other.

Further, according to the present invention, in order to be able to dispense a very small amount of a sample or a reagent, as described in claim 4, a flow for connecting and connecting each of the nozzles and the pump. A pressure holding portion for holding the internal pressure of the pipe and a high-speed plunger valve (a solenoid valve that opens and closes the orifice at a high speed with a minimal plunger) are provided in the passage, and the pressure of the pressure holding portion is adjusted by a pressure adjusting means. Control to maintain a constant pressure via the high speed plunger valve,
When the sample or the reagent is dispensed, it is configured to open and close at high speed and dispense by pressure. In this case, as described in claim 5, the high-speed plunger valve sets the voltage / current during its operation to the rated voltage / current,
It is desirable to reduce the holding voltage and current immediately after the operation to prevent malfunction and deterioration of durability due to heating.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will now be described in detail based on an embodiment example shown in the accompanying drawings.

As shown in FIGS. 1 and 2, the automatic trace amount blood analyzer 1 according to this embodiment takes the blank value of the reaction container 6, so that the first reagent corresponding to the measurement item is previously stored in the reaction container 6. After the blank value measurement is completed, the operation is controlled so that the sample is dispensed in a predetermined amount.

The specific structure of the automatic trace blood analyzer 1 controlled in this way is as follows: a sample container transfer device 3 for holding a plurality of sample containers 2 in a loop, and a sample suction position A.
A sample dispensing device 4 for sucking a small amount of sample from the sample container 2 and an adhering liquid wiping device 5 for wiping the sample adhering to the outer surface of the sample nozzle PA of the sample dispensing device 4 at the adhering liquid wiping position B. And a reaction table 7 for holding and rotating a plurality of reaction containers 6 into which the sample sucked by the sample nozzle PA is dispensed at a sample dispensing position C, and a first reagent and a second reagent corresponding to a measurement item. A reagent dispensing device 8 for dispensing the reagent into the reaction container 6 at the reagent dispensing position D, and a reagent container 9 accommodating the first reagent and the second reagent corresponding to the measurement item in a loop shape.
The reagent supply device 10 that is rotationally transferred to the reagent dispensing position E or the second reagent dispensing position F, and the sample attached to the outer surface of the reagent nozzle PB of the reagent dispensing device 8 is wiped at the attached liquid wiping position G. Adhesive reagent wiping device 11, a stirring device 12 that stirs the mixed state of the sample and the reagent stored in the reaction container 6 at a stirring position H, and the reaction liquid is irradiated with light having a wavelength corresponding to the measurement item. An optical analyzer 13 that irradiates at a measurement position I and measures the amount of transmitted light, and an arithmetic circuit 14 that converts the light amount data measured by the optical analyzer 13 into a voltage and performs arithmetic processing to perform quantitative analysis of measurement items. , A control circuit (CPU) 15 for driving and controlling the above-mentioned mechanisms so as to continuously operate organically.
And a printer 16 that prints out the measurement data in association with the sample information. Incidentally, FIG.
Reference numeral 17 denotes a power supply unit, 18 denotes a motor drive circuit for each mechanism, 19 denotes a temperature control circuit for controlling the temperature management of reagents and the constant temperature of the reaction container, and 20 denotes an operation unit.

In this embodiment, the automatic micro blood analyzer 1 having the above-mentioned configuration has 20 simultaneous biochemical tests and 80 continuous test items (maximum 20 items, 4 samples). The amount of sample used is 30 μl / 20 items (required sample amount is 100 μl), the amount of reagent used is 50 μl (1 reagent system) to 100 μl (2 reagent system) / item, the reaction table is exchange system (disposable type), and discrete single line multiple The item analysis method is adopted, the one-point method and the rate method are adopted as the analysis methods, the absolute method and the relative method are adopted as the measurement methods, and the analysis required time is 12.
5 minutes / maximum 20 items and reaction time is set to 7.5 minutes. Of course, the present invention is not limited to the specifications of this embodiment, and various known analysis methods and methods can be adopted, and the required analysis time, reaction time, sample amount and reagent The amount can be increased or decreased according to the purpose of use.

The sample container transfer device 3 employs a turntable system and is configured to send the sample container 2 to the sample dispensing position A at an intermittent pitch at regular intervals.
The number of sample sets is 6 for the calibration curve and 6 for general samples. The sample identification is managed by the table number.

The sample dispensing apparatus 4 transfers the sample nozzle PA from the sample suction position A to the reaction container 7 which has reached the sample dispensing position C of the reaction table 6 through the adhering liquid wiping position B and sucks the sucked sample. After dispensing the required amount, reattachment liquid wiping position B
Transfer to

The adhering liquid wiping device 5 is constructed, for example, as shown in FIG. 3, so that a long liquid absorbing tape 21 is wound from a roller 22 to a winding roller 23 by a predetermined amount at a predetermined timing. A cleaning trough 24 is arranged between the rollers 22 and 23.

With this structure, when the sample nozzle PA that has sucked the sample is transferred to the adhering liquid wiping position B and descends at the position B, the nozzle PA may come into contact with or break through the liquid absorbing tape 21. Then, since the sample adhered to the outer surface of the nozzle PA is sucked up, the dispensed amount can be accurately controlled. This is the most important control technique especially for an apparatus for analyzing an extremely small amount of blood.

The nozzle PA is moved to the position B before the sample dispensing and is subjected to the wiping work of the adhering sample, but is moved to the same position B again in the washing step after the sample dispensing, as shown in FIG. As shown in FIG. 5, when the cleaning trough 24 descends and receives the cleaning work inside and outside the nozzle PA, and then ascends again, as shown in FIGS. 5 to 8, the cleaning water attached to the outer surface of the nozzle PA. Since the liquid is wiped by the liquid absorbing tape 21, there is no concern that the wash water will be mixed into the sample and diluted when the next sample is sucked.

The reaction vessel 6 is made of transparent resin or glass and has a bottomed rectangular tube shape. In this embodiment, after all 80 reaction vessels 6 held on the reaction table 7 are used, Each reaction container 6 is removed by hand, and a new reaction container 6 is set on the reaction table 7. Reaction vessel 6 used
Wash and reuse.

The reaction table 7 is 360 in this embodiment.
Each reaction container 6 is sequentially rotated from the sample dispensing position C through the reagent dispensing position D and the stirring position H to the optical measurement position I by rotating at a pitch of ± 1 reaction container.
In the reaction table 7, the temperature control circuit 19 controls the reaction liquid of the sample and the reagent so as to maintain a constant temperature state, that is, 37 ° C. ± 1 ° C.

The reagent dispensing apparatus 8 having the reagent nozzle PB for sucking the reagent is arranged at the reagent dispensing position D for the first reagent or the second reagent corresponding to the measurement item in the reaction container 6 into which the sample is dispensed.
The reagent nozzle PB sucks a required amount of the first reagent or the second reagent corresponding to the measurement item at the first reagent suction position E or the second reagent suction position F, and then attaches it. The reagent is transferred to the reagent wiping position G, and the reagent nozzle PB is moved to the position G.
The reagent adhering to the outer surface of the is wiped off. This makes it possible to accurately control the amount of the dispensed reagent.

The reagent container 9 accommodating the first reagent and the second reagent is the first reagent container 9A inside the container in this embodiment.
Although the one-bottle type in which the second reagent storage portion 9B is formed on the outer side is formed, a container can be formed separately and used.

In the reagent supply device 10, the reagent container 9 accommodating the reagent corresponding to the measurement item is placed at the first reagent dispensing position E.
Alternatively, it is transferred to the second reagent dispensing position F by forward / reverse rotation control. In the present embodiment, as described above, in order to measure the blank value of the reaction container 6, the first reagent corresponding to the measurement item is dispensed in the reaction container 6 in a required amount in advance, and after the blank value is measured, A predetermined amount of a sample is discharged and dispensed into the reaction container 6, and in the case of two-reagent system measurement, a second reagent corresponding to the measurement item is then dispensed in a predetermined amount.

The adhering reagent wiping device 11 is constructed so that, like the adhering liquid wiping device 5, the long liquid absorbing tape 21A is wound from the roller to the winding roller by a predetermined amount at a predetermined timing. The cleaning trough 24A is disposed between the rollers.

With this structure, when the reagent nozzle PB sucking the reagent corresponding to the measurement item is transferred to the adhering reagent wiping position G and descends at the position G, the nozzle PB
When the liquid comes into contact with or breaks through the liquid absorbing tape 21A, the reagent attached to the outer surface of the nozzle PB is absorbed,
The amount of reagent dispensed can be controlled accurately.

Further, the nozzle PB is transferred to the position G after the first reagent suction and the second reagent suction and is subjected to the wiping work of the adhering reagent, and again at the same position G in the washing step after the reagent dispensing. To the cleaning trough 24A, and after receiving the cleaning work inside and outside the nozzle PB by the cleaning trough 24A,
When rising again, the cleaning water adhering to the outer surface of the nozzle PA is wiped off by the liquid-absorbing tape 21A, so that the sample is mixed in the reagent and a quantitative error occurs, or cross contamination occurs. It can be reliably prevented, and there is no concern that the reagent will be diluted by the wash water.

The stirrer 12 is a stirrer in which a stirring rod 25 is inserted into the reaction solution for stirring in order to homogenize the reaction between the sample and the reagent dispensed in the reaction container 6, and stirring is performed. In order to prevent cross contamination, the stirring rod 25 which has been finished is washed and wiped by a stirring rod wiping device 26 having the same configuration as the attached liquid wiping device 5. Although not shown in particular, the stirring device 12 also includes
A wiping device having the same structure as the adhering liquid wiping device 5 for wiping off the reaction liquid adhering to the outer surface of the stirring rod 25 after use is additionally provided.

The optical analyzer 13 forming the detection section or the observation point is constructed by a diffraction grating method (a wavelength conversion method by a filter may be used), and includes a light source 27 and
The measurement light emitted from the light source 27 and transmitted through the reaction container 6 is configured to be separated by a diffraction grating and received by a plurality of light receiving elements (not shown) arranged on the focal point of the diffraction grating. Among these, the output from the light receiving element corresponding to the measurement item is sent to the arithmetic circuit 14.

The calculation circuit 14 calculates the output value based on a predetermined calculation processing method, and the calculated value is calculated by the printer 16
Printed out from.

The control circuit (CPU) 15 is a circuit for controlling all of the automatic analyzer, and at the same time, has an external CP.
The control circuit 15 is also provided with a RAM CPU board (not shown) for recording the values calculated by the arithmetic circuit 14, which controls communication with the U. This RAM CPU
The board automatically stores and saves measurement data, reaction time course data and trouble data of each reaction container 6, and is configured to have a storage capacity of at least 1 kilomegabyte or more. An inspection report can be quickly created in real time by outputting from an external output terminal to an external computer.

Next, the sample dispensing system of the automatic trace blood analyzer 1 according to this embodiment will be described with reference to FIGS. 3 to 10. In the figure, reference numeral 25 indicates a container containing wash water W, 26 is a flow path 34 and a flow path 35 having ports a and b.
Alternatively, a flow passage switching valve for switching between the flow passage 37, 27 is a pump for sucking and pressurizing the sample to wash the pressure holding portion 20 of the suction system, 28 is a pump drive motor, and 29 is a pump control circuit. , 30 is a pressure-holding portion formed in the middle of the flow path 35, for example, in the form of a coil, 31 is a precision pressure gauge for measuring the pressure in the flow path 35, and 32 is the communication between the flow paths 34 and 35. A pressure detection circuit for detecting the pressure in the flow path 36
Reference numeral 33 denotes a sample nozzle PA and a high-speed plunger valve interposed in the middle of the pressure holding unit 30, respectively.

FIG. 3 shows a state after the initialization of the sample dispensing system configured as described above, and the analysis of the sample is started from the initialized state. First, in the cleaning operation of the suction system shown in FIG.
Then, the pump 27 is set in the communication state by the suction operation. Then, when this suction operation is completed, the flow path switching valve 26 is switched to connect the flow path 34 and the flow path 35 in communication, and the suctioned cleaning water is flow path 34 → flow path switching valve 26 →
Flow through the flow path 35 → pressure holding unit 30 → high-speed plunger valve 33 that has been turned ON → sample nozzle PA to wash the inside of the suction system flow path. Wash water that has been washed is washed trough 2
Drained to 4.

Next, the operation shown in FIG.
Shows a state in which the sample moves from the washing position B to the sample suction position A to suck the sample. In this state, the flow path 3
4 is connected to the flow path 35 via the flow path switching valve 26, and the high speed plunger valve 33 is set to the ON state (flow path communication state). And the pump 2
Before sucking the sample 38 from the sample container 2, the sample No. 7 operates slightly to suck air into the sample nozzle PA to fractionate the sample so as not to be diluted with washing water.
Is controlled so as to suck a required amount.

In FIG. 6, the sample nozzle PA rises from the state of FIG. 5, and the sample 38 adhering to the outer surface of the nozzle PA at the cleaning position B is wiped with the liquid absorbing tape 21 and then moved to the sample dispensing position C. 3 shows the state of each flow path when it is transferred. In this state, the flow path 34 is connected to the flow path 3 via the flow path switching valve 26.
5 is connected to the high speed plunger valve 3
No. 3 is set to the OFF state (flow path cutoff state).

FIG. 7 shows a sample dispensing state. In this state, the flow path 34 passes through the flow path switching valve 26 and the flow path 35.
The pump 27 operates in the discharge side to increase the pressure in the pressure holding unit 30 and is controlled by a feedback signal from the pressure detection circuit 32 before the pressure reaches the set discharge pressure. Information is sent to the circuit 29 to control the operation of the pump 27. Then, in the state where the pressure control unit 30 is pressurized to a predetermined pressure, the time (mmsec, n) corresponding to the sample dispensing amount of the high-speed plunger valve 33 is set.
After being opened in units of sec, it is controlled by the drive control circuit so as to be instantly turned off (flow path cutoff state).
As a result, an accurate amount of the sample is dispensed even if the amount of the sample is very small. The dispensing operation may be performed continuously for 20 items within the dispensing cycle time, or may be performed for each item by suction / discharge.

FIG. 8 shows the cleaning operation state after the sample dispensing work is completed, and the sample nozzle PA after the sample dispensing work is transferred from the sample dispensing position C to the cleaning position B. After that, the pump 27 operates, the high-speed plunger valve 33 continues to be in the ON state, and the cleaning water W filled in the flow passages 34 and 35 cleans the inside of the nozzle PA to the cleaning trough 24. Be drained.

In the ultratrace blood automatic analyzer 1 according to this embodiment, in order to prevent cross contamination more reliably, as shown in FIG. 9, the washing work is performed again after the washing work is completed in FIG. Is configured.

After the rewashing operation is completed in this way, the sample nozzle PA is reset to the sample dispensing cycle operation end state shown in FIG. 10 to prepare for the next sample dispensing operation.

In the ultratrace blood automatic analyzer 1 according to this embodiment, as described above, even in the case of a trace amount of sample, the flow passages 34 and 35 are pressurized, and in this state, the high-speed plunger valve 33. It is possible to reliably discharge and dispense a fixed amount (required amount) of a small amount of sample by instantaneously controlling the opening and closing. Further, since the liquid adhering to the outer surfaces of the nozzles PA, PB and the stirring rod is wiped with the disposable type liquid absorbing tape 21, a quantitative error due to the adhering liquid on the outer surface of the nozzle and the stirring rod is prevented. In addition, it is possible to completely prevent cross contamination.

Further, in the ultratrace blood automatic analyzer 1 according to this embodiment, the high-speed plunger valve 33 is normally operated at DC 12V or 24V, but when it is energized for a long time, high heat is generated, so the same voltage is applied. After being operated at 1, the heat generation can be significantly reduced by lowering the holding voltage and current.

[0045]

EFFECT OF THE INVENTION Since the ultratrace blood automatic analyzer according to the present invention is configured as described above, it is possible to perform the same test as the conventional automatic analyzer with a trace amount of sample and a small amount of reagent. It can be easily implemented for infants and critically ill patients,
In addition, it can be applied to blood analysis of pets that can collect only a small amount of blood, and has an excellent effect that it can be provided at a low cost with a simple configuration.

[Brief description of drawings]

FIG. 1 is a perspective view of an ultratrace blood automatic analyzer according to an embodiment of the present invention.

FIG. 2 is a mechanism explanatory view showing the principle of the same micro blood automatic analyzer.

FIG. 3 is a flow path system piping explanatory diagram showing a state after initialization in the sample dispensing system of the same type of micro blood automatic analyzer.

FIG. 4 is an explanatory diagram of a cleaning operation of the sample dispensing system.

FIG. 5 is an explanatory diagram up to the sample suction operation of the sample nozzle of the sample dispensing system.

FIG. 6 is an operation explanatory view of the same sample nozzle until dispensing.

FIG. 7 is an operation explanatory view showing a dispensing state of the sample nozzle.

FIG. 8 is a diagram illustrating cleaning of the sample suction system of the sample nozzle.

FIG. 9 is an explanatory diagram of rewashing operation of the sample dispensing system.

FIG. 10 is a diagram illustrating the end of one cycle operation of the sample dispensing system.

[Explanation of symbols]

A Sample suction position B Adhesive liquid wiping (cleaning) position C Sample dispensing position W wash water PA sample nozzle 1 Micro blood automatic analyzer 2 sample containers 3 Sample container transfer device 4 Sample dispensing device 5 Adhesive wiping device 6 reaction vessels 7 Reaction table 8 Reagent dispensing device 9 Reagent container 11 Adhesive reagent wiping device 13 Optical analyzer 14 Arithmetic circuit 15 Control circuit (CPU) 21 Liquid absorption tape 24 cleaning trough 27 pumps 30 Pressure holding part 32 Pressure detection circuit 33 High-speed plunger valve

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 1/28 Y F term (reference) 2G045 AA01 AA15 CA26 CB03 2G052 AA30 AD26 AD46 CA03 CA20 CA28 CA32 CA35 EB12 FB02 FB07 FC05 FC09 FC11 FC15 FC18 GA11 HA18 HB10 JA00 JA03 2G058 AA05 BB02 BB09 BB15 BB27 CB04 CC14 CD04 CE08 EA02 EA04 EA14 EB01 EC11 ED03 ED21 ED25 FA02 FB03 GD06 GE06 GE06 GE06 GC02 GC02 GC02 GC02

Claims (5)

[Claims] 1. A predetermined amount of reagent corresponding to a measurement item is sucked by a reagent nozzle of a reagent dispensing apparatus, and then a reagent adhering to the outer surface of the reagent nozzle is wiped by an adhering reagent wiping unit, and then the reagent is wiped. After moving the reagent nozzle to the reaction container and dispensing the aspiration reagent, after aspirating the sample required for the measurement item from the sample container to the reaction container in which this reagent has been dispensed, with the sample nozzle of the sample dispensing device , The sample adhered to the outer surface of the sample nozzle is wiped by the adhering liquid wiping unit, then the sample nozzle is transferred onto the reaction container, and the sample amount corresponding to the reagent item in the reaction container is discharged. An automatic trace amount blood analyzer, which is configured to perform optical measurement at a predetermined wavelength on a reaction sample that has been heated and reacted in a constant temperature state by stirring with a stirring rod of a stirring device after dispensing. 2. A liquid-absorbing tape is provided on the adhering reagent wiping unit, the adhering liquid wiping unit, and the stirring rod wiping unit, and the liquid-absorbing tape is wound by a fixed amount after absorbing the liquid. The ultratrace blood automatic analyzer according to claim 1, which is characterized in that. 3. The flow path connecting the nozzles and the pump for communication is filled with washing water in a normal state, and when the sample or the reagent is sucked, the pump is operated to suck the sample or the reagent. The bubbles are interposed between the cleaning water and the cleaning water so that they do not mix with each other.
Alternatively, the ultratrace blood automatic analyzer according to claim 2. 4. A pressure holding part for holding the pipe internal pressure and a high-speed plunger valve are provided in a flow path connecting the nozzles and the pump for communication, and the pressure of the pressure holding part is passed through a pressure adjusting means. It is controlled to maintain a constant pressure, and the high-speed plunger valve opens and closes at high speed when dispensing a sample or reagent, and continuously dispenses a very small amount of sample with the pressure of the pressure holding unit. However, the reagent is dispensed by a required amount, and the ultratrace blood automated analyzer according to any one of claims 1 to 3. 5. The automatic trace blood analyzer according to claim 4, wherein the operating voltage / current of the high-speed plunger valve is a rated voltage / current and is lowered to the holding voltage / current immediately after the operation.