JP2003304796A - Method of preventing barnacles from sticking - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To immediately detect whether the development of larvae of barnacles of specific kinds occurs or not in the marine water to be used and inhibits the cypris-form larvae of barnacles from sticking by taking a widely known means suitably. <P>SOLUTION: The larvae in the sticking period of barnacles including Balanus amohitrite, Balanus eburneus, Megabalanus volcano, Balanus reticulates, rock barnacle, Balanus albicostatus, Balanus imorovisus, collected from a prescribed volume of sea water are irradiated with exciting light of 400-440 nm wavelength and the fluorescent distribution pattern of the light emitting in ≥475 nm wavelength are input to a computer as digital image information. The information is compared with the body fluorescent light distribution patterns that is intrinsic to individual species of barnacles that are registered to the computer in advance. On the basis of the species of pattern-matched barnacle, the number of the larva bodies in a unit volume of sea water and the species to be controlled are decided. Then, a barnacle attachment-controlling treatment is carried out suitably against the target species for a needed period. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for controlling damage caused by adhesion of barnacles to an undersea structure, and more particularly to a method for controlling adhesion of barnacles, which can control the adhesion of barnacles by type.

[0002]

2. Description of the Related Art Organisms that adhere to or adhere to the foundations of underwater rocks or concrete walls are called adhering organisms (sometimes called fouling organisms), but they belong to the crustacea and the amphipoda. Barnacles, which are pods, are typical marine adherent organisms.

It is known that barnacles are composed of many species from inner bay species to pelagic species and have a habitat peculiar to species depending on the environmental conditions and foundation of the sea area.

The outline of the stages involved in reproduction in the life history of barnacles is as follows. That is, after mating and mating between adherent adults, a floating phase of Nauplius larvae is hatched, and after this Nauplius larvae repeatedly molts, it becomes a cypris larvae in an adhering phase, and further cypris larvae adhere to the base, It transforms into a young barnacle. This breeding season is unique to each species.

By the way, as shown in FIG. 1, the cypris larva is a larva having a transparent spindle-shaped transparent upper (shell) 1 which is laterally compressed, and has a pair of first antennas 2 in front of the abdomen and a second half of the abdomen. Six pairs of chest limbs 3 extend from the inside of the upper 1 to the inside.

The first antenna 2 is an organ having a sucker-shaped tip for attachment, and an adhesive substance (quinone cross-linking protein) secreted from the cement gland 4 through the cement tube 5 is attached to the surface of the attached organ. It is secreted and adheres to the base. In addition, the cypris larvae repeat their approach to and detachment from the substrate to check the suitability for the substrate, and during that time, detachable temporary attachment is made, and then permanently fixed to the finally determined fixing point. In the figure, reference numeral 6 is an oil cell, 7 is a compound eye, and 8 is a chest.

[0007] A large amount of such barnacles adhere to various offshore structures, ship bottoms, and water distribution pipes such as heat exchangers and condensers of seawater intake facilities such as power plants. Speed decrease, fuel consumption increase, intake pump load increase,
It may cause various damages such as cooling efficiency deterioration and narrow tube blockage.

[0008] In that case, if the appearance situation of each barnacle species is grasped and the barnacle species that are the most damaged in the target sea area are eradicated, that is, the adhesion control measures are concentrated only on the breeding period of the specific barnacle species. If it is possible, the damage can be avoided more efficiently.

[0009]

However, because the morphology of the cypris larvae, which are the adhering stage larvae of barnacles, is very similar to each other, the morphology between species is very similar. There was a difficulty in species discrimination by carefully observing the external shape and the morphology of the characteristic part, and there was no simple means that did not require skill.

In such discrimination of species by microscope observation that requires a long time, it is impossible to make a determination for taking effective control measures almost at the same time as sampling.

Therefore, in facilities such as seawater utilization plants, chemicals are continuously injected into seawater for a whole year without limiting the period of adhesion of barnacles of a particular species that causes large damage, or a thin tube of a heat exchanger is regularly used. We carry out so-called "ball cleaning measures" in which sponge-like balls for cleaning the inner surface are put into a thin tube, and cleaning work such as brush cleaning in an out of service state. This caused a drop in operating efficiency due to suspension of operation.

Therefore, an object of the present invention is to solve the above-mentioned problems, to promptly detect whether or not the larvae of a specific species appear in the seawater to be used, when the inspection is necessary, and further Controlling the attachment of barnacle Cyprus larvae by applying control means, that is, identifying barnacles with significant damage at any time, and concentrating effective pre-attachment control measures on the barnacle Cyprus larvae. Is to do so efficiently and efficiently.

[0013]

[Means for Solving the Problems] The inventors of the present application, for the cypris larvae of various barnacles obtained by artificial rearing, the fluorescence self-luminous property of the larvae under the irradiation of excitation light in each wavelength range, the emission shape, As a result of studies on the detection of larvae by fluorescent staining and the like, a method for immediately detecting barnacle Cyprus larvae and determining the type thereof was developed, and the present invention was completed.

That is, in order to solve the above-mentioned problems, in the invention of the present application, the adhering stage larvae of barnacles collected from a predetermined amount of seawater are irradiated with excitation light, and the fluorescence distribution pattern of each light-emitting individual is determined. It is input to a computer as digital image information, and this information is compared with in-vivo fluorescence distribution pattern recognition information unique to the species registered in advance in the computer, and the species of barnacles and their species in which these fluorescence distribution patterns are matched. The method for controlling the adhesion of barnacles comprises determining the species to be controlled from the number of individuals per unit volume of the seawater and subjecting the species to be controlled to the adhesion controlling treatment for a required period.

In the above method, as a pretreatment for the step of irradiating with excitation light, there can be adopted a method having a step of fluorescent labeling of sugar chain fluorescent labeling of larvae of the barnacles with fluorescent labeled lectin.

The method for controlling the adhesion of barnacles according to the present invention comprises:
Irradiating excitation light to various barnacle Cyprus larvae,
At that time, the specific fluorescence emission site and emission shape of the larva can be used as a newly-discovered taxonomic feature, so that the immediate detection of the larva and the determination of its type can be performed easily and reliably.

That is, it was found that when various barnacle Cyprus larvae are irradiated with excitation light, they emit light with a fluorescence distribution pattern peculiar to the species.

Usually, many plankton species have autofluorescence in the body, but few are useful for discrimination of species. However, the barnacle Cyprus larvae emit specific fluorescence having a pattern capable of clearly discriminating species, and there is little individual difference in the pattern of such fluorescent site and luminescent shape, and it can be sufficiently utilized as an identification marker.

Further, before irradiation with excitation light, the sugar chains on the body surface of the larvae can be dyed with a fluorescently labeled lectin to be fluorescently labeled.

Specifically, the preferable range of the wavelength of the excitation light applied to the adhering stage larvae of barnacles is 400 to
440 nm and the emission wavelength of the emitted fluorescence is 47
It is preferably 5 nm or more.

In this way, most living organisms other than barnacles do not emit fluorescent light or become only extremely weak. Therefore, noise-like fluorescence emitted by other plankton is eliminated to distinguish the species of barnacles. Can be done reliably. In addition, it is possible to distinguish the intensity of light emission, the head of cypris larva as a light emitting site, the rear part or the whole body, and the light emitting unit such as speckles or smaller fine particles, and light emission of the whole body without particles. The species can be identified by the combination of these characteristics.

In particular, the barnacles are Tatemima barnacles,
American Barnacle, Red Barnacle, Sankaku Barnacle,
In the case of two or more barnacles selected from barnacles, which include barnacles, barnacles, barnacles, barnacles, and barnacles in Europe, the species can be more reliably identified.

In order to perform type determination without subjective error based on objectively evaluated criteria, it is necessary to perform image analysis using a computer and automatically identify this. At that time, the fluorescence distribution pattern is taken into the computer as digital image information, and this information is compared with the fluorescence distribution pattern recognition information unique to the species which has been registered in the computer in advance. At that time, general-purpose image analysis software can be used. For example, NIH Imag manufactured by National Institutes of Health
e, Mac Scope or Win ROOF manufactured by Mitani Corporation.

The comparison of the fluorescence distribution patterns is carried out by comparing the species-specific in-vivo fluorescence distribution pattern recognition information registered in advance in the computer with the fluorescence distribution pattern of the digital image for inspection. Depending on the probability of matching, the species of barnacles and the number of individuals per unit seawater volume of the species are calculated. From this result, the measurer can determine the target species for control based on general knowledge or unique empirical rules, and perform effective adhesion control treatment on barnacles only for the effective period required for this species. it can.

According to the present invention, it is possible to determine the species of barnacle Cyprus larvae, which could not be dealt with immediately by microscopic observation with ordinary wavelength light in the prior art, and to perform automatic identification by image analysis of the biomass estimation, and the barnacles. It is possible to improve the efficiency of the control measures focused on the breeding (adhesion) time of the species, and to perform the control measures focused only on the breeding time of the specific species that causes the most damage in the target sea area.

[0026]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the method for immediate detection and type determination of barnacle Cyprus larvae of the present invention will be described below in detail with reference to the accompanying drawings.

As shown in FIG. 1, the adhering-stage larvae of barnacles (the drawing is a barnacle) is called a cypris larvae, and the sample knows the population density (the number of individuals per unit amount of seawater). In order to do this, collect from a predetermined amount of seawater.

The collection can be carried out by filtering a fixed amount of seawater with a net from a plankton net equipped with a drainage meter or a pump pumping up seawater. Also,
As an automatic collection method, a window made of a glass cell is provided in the flow path of the pumped seawater, and the cypris larva is temporarily attached to this cell to collect the cypris larva, or the cell is photographed. It can also be recorded as image data.

In any of the collection methods described above, attachment-stage larvae of barnacles collected in cells or plankton nets are subjected to sugar chain fluorescent label staining, if necessary, and then irradiated with excitation light to emit fluorescence distribution. Cooled pattern CC
Take a picture with a D camera or digital camera, and input it to a computer as digital image information.

To irradiate the excitation light, it is preferable to use, for example, an epi-illumination fluorescence stereomicroscope. Specifically, each excitation filter or scanner is used to irradiate the glass container filled with seawater with the excitation light of a predetermined wavelength. Recording may be performed with a cooled CCD video camera or the like attached to an epi-fluorescence stereomicroscope through a predetermined absorption filter.

The wavelength of the excitation light with which the larvae of the barnacles are attached is preferably 400 to 440 nm.

By observing the emitted fluorescence having a wavelength of 475 nm or more, there is almost no fluorescence emitted by organisms other than barnacles, and only fluorescence that can clearly distinguish the species of barnacles can be recognized.

The information on the emitted fluorescence distribution pattern is compared with the species-specific fluorescence distribution pattern recognition information registered in advance in the computer. The characteristic fluorescence distribution pattern actually obtained is as shown in the following description and FIGS. (A) Larvae of Aedes acupuncture: As shown in FIG. 2 (a), the head and back of the larvae emit strong light in spots. (B) American barnacle larva: As shown in FIG. 2 (b), the head and the rear end emit light strongly in a wide range. (C) Sankaku barnacle larva: As shown in FIG. 2 (c), the entire body emits light, and the head emits fine-grained light. (D) Larvae of red barnacles: The entire body emits light as shown in FIG. 2 (d). (E) Greater barnacle larva: As shown in FIG. 3 (a), the entire body emits light weakly and the lower edge of the body emits patchy light. (F) Larval Acupuncture Larva: As shown in FIG. 3 (b), the head and tail emit strong light in a mesh pattern. (G) Larval barnacle larva: As shown in FIG. 4 (a), the entire front part of the body emits light in a lump form. (H) White-breasted barnacle larvae: As shown in FIG. 4 (b), the front and rear edges of the body emit edging light. (I) European barnacle larvae: Small ellipsoidal discs at the front and rear edges of the body emit light, as shown in FIG. 4 (c).

The species to be controlled is determined from the species of barnacles matching these fluorescence distribution patterns and the number of individuals per unit seawater volume of the species.

By registering the recognition information on the fluorescence distribution pattern peculiar to the species in advance in a computer, the species of barnacles matching the fluorescence distribution pattern existing in the test seawater and the unit seawater volume of the species. Can be obtained.

Based on such information, the most dominant species is set as the control target species, or when the less seriously damaging species dominate, this species is ignored and the specific heavily damaging species are controlled. The target species may be determined based on the manager's rule of thumb.

The seawater dominated by the control target species thus determined is subjected to the adhesion control treatment for a required period.
Adhesion control treatment may be carried out by adopting a method conventionally used for adherent organisms or any other suitable control method.In general, by adding a control chemical such as sodium hypochlorite solution to seawater. is there.

By the way, Table 1 below shows a general example of the adhesion time depending on the type of barnacles and the ocean current system.
It should be noted that the warm-current barnacles are an example of the main adhesion time in the Kansai region, and the cold-current barnacles are an example of the main adhesion time in the Tohoku region.

[0039]

[Table 1]

[0040]

[Examples] [Detection of fluorescence distribution pattern in body peculiar to barnacles species] 1-1) Various barnacles Cypris larvae obtained by artificial breeding of test organisms were exposed to high-concentration magnesium ion seawater to temporarily After stopping the movement of the larvae, they were placed in a petri dish together with the seawater. 1-2) Observation of larvae's autofluorescence Using an epi-illumination fluorescence stereomicroscope, the larvae in the petri dish are irradiated with excitation light of each wavelength, and the larvae's autofluorescence and luminescence of various larvae are observed through each absorption filter. Observations were made. The excitation filter wavelength and the absorption filter transmission wavelength of the irradiated light are as follows. Excitation filter wavelength 330-385 nm, absorption filter transmission wavelength
420nm or more Excitation filter wavelength 400-440nm, Absorption filter transmission wavelength
475nm or more Excitation filter wavelength 400-440nm, Absorption filter transmission wavelength
470-495nm Excitation filter wavelength 460-490nm, Absorption filter transmission wavelength
510-550nm Excitation filter wavelength 510-550nm, Absorption filter transmission wavelength
590nm or more 1-3) Results Barnacles Cypris larvae have an excitation filter wavelength of 400-4
Irradiation of excitation light of 40 nm, absorption filter transmission wavelength of 475 nm or more
Specific self-luminous emission was observed under fluorescence-accepting conditions, and no luminescence was observed in other wavelength regions, particularly in the excitation wavelength region of 460 nm or more. The self-luminous shapes of larvae are shown in Fig. 2a, b, c, d and Fig. 3.
As shown in FIGS. 4a, 4b, 4c, 4a, 4b, 4c, and 4d, peculiar morphologies of various barnacles are shown.

As shown in FIG. 2 (a), the larvae of the Tadema barnacles larvae emitted strong spot-like light at the head and back of the larvae.

As shown in FIG. 2 (b), the barnacle larvae of the barnacles emitted intense light over a wide area at the head and rear end.

As shown in FIG. 2 (c), in the larva of Sankaku barnacle, the whole body part emitted light, and the head part emitted fine particles.

As shown in FIG. 2 (d), the entire body of the red barnacle larvae emitted light.

As shown in FIG. 3 (a), in the larvae of the barnacles, the whole body part emitted weak light and the lower edge of the body emitted patchy light.

As shown in FIG. 3 (b), the head and tail portions of the larvae of Amaranthus acupuncture brilliantly emitted light in a mesh pattern.

As shown in FIG. 4 (a), in the larvae of the barnacle, the entire front of the body emitted light in a lump form.

As shown in FIG. 4 (b), in the larva of the white barnacle, the front edge and the rear edge of the body emitted light in a edging manner.

As shown in FIG. 4 (c), in the European barnacles, the small elliptical discs at the front and rear edges of the body emitted light.

That is, by utilizing the specific autofluorescence emission of larvae under irradiation of excitation light of a specific wavelength, immediate detection and type determination of barnacle larvae, which was impossible only by morphological observation with ordinary light, can be performed. It was possible. Also, automatic identification by image analysis is possible.

[Detection by Fluorescent Labeling Staining] 2-1) Put Tadima Fujitsubo Cypris larvae together with seawater in a plastic petri dish with a cover glass attached to the bottom of the test organism and let stand for several hours to leave the larvae on the cover glass. It was first deposited. 2-2) Staining and observing fluorescence-labeled Cypris larvae that have been primarily attached to the cover glass are fixed or left unfixed, and fluorescence-labeled lectin (LCA-FIT) is used.
Direct staining with C) was performed. Staining conditions: density: 1
00-1000 times TBS dilution, time: 10 minutes to 4 hours, and the stained larvae were observed under an epifluorescence microscope (excitation light wavelength 460-490 nm, absorption filter transmission wavelength 510 nm or more). 2-3) Results In the case of LCA-FITC staining, luminescence was confirmed in the whole body part of the cypris larva and the whole adherent organ part even after staining at a concentration of 1/1000 × 10 minutes. At the same time, non-specific luminescence of various adherents such as organic matter on the surface of the cover glass was confirmed, but the luminescence of Cyprus larvae is very characteristic in size and shape, and it is different from other species' adherents and organisms. It was fully possible to discriminate.

That is, it was possible to immediately detect the barnacle Cyprus larvae by observing fluorescence after staining with a specific fluorescent label. Therefore, similar to the case of autofluorescence, it is possible to automatically identify the cypris larvae by image analysis.

[Example] A sample seawater intake pipe was installed in the sea area near the cooling seawater intake of a coastal plant (Kansai region), and 1000 liters of seawater pumped by a pump was periodically collected, and this was used as a zooplankton. It is filtered and collected with a collection net (mesh 0.1 mm), and this is 200 mM Mg.
^The larvae were temporarily stopped from swimming by exposure to ²⁺ seawater and samples containing the larvae were placed in a petri dish with seawater.

The collected sample containing the larval stage of the barnacles attached to the barnacle was irradiated with excitation light having a wavelength of 400 to 440 nm by using an epi-fluorescence stereomicroscope (manufactured by Olympus Corp.), and a wavelength of 47
The fluorescence emission digital image obtained through an absorption filter that transmits fluorescence of 5 nm or more was imported as data into image analysis software (NIH Image) of a personal computer.

Prior to the acquisition of this image data, the fluorescence distribution pattern peculiar to the species obtained by the above-described experiment (that is, the larvae of the Tadima barnacle larvae emit strong speckled light at the larval head and rear). In the larvae, the head and the rear end emit intense light over a wide range, in the larvae of Sankaku barnacles, the entire body part emits light, and in addition, the head emits fine-grained light, and in the larvae of red barnacles, the entire body part emits light. The larvae emit light weakly throughout the body and patchy emission at the lower edge of the body.The head and tail of the Sarasa barnacle larvae emit a strong net-like light. White barnacle larvae emit light with edging at the leading and trailing edges of the body, while European barnacles have small oval discs at the leading and trailing edges of the body. Light to.) Recognition information necessary for specific detection and type identification of barnacles attached life larva by image analysis software (NIH Image) from which had been registered in advance in the personal computer.

The recognition information is image analysis software (NI
H Image), which is information obtained by the processing performed according to the program, specifically, density gradation processing, smoothing processing, sharpening processing, masking, etc., if necessary, and further image binarization processing. At the same time, noise other than the target area is appropriately removed, and the image pattern of the counting target area is recognized.

As the recognition information registered in the computer, if recognition information of one or more species to be counted is designated, per unit volume of seawater of one or more species of barnacles attached period larvae designated from the digital luminescence image of the sample. The number of individuals can be automatically measured.

An image processing process and an example of automatic counting are shown in FIG. 5 and FIG. 6 (both of which are traces of micrographs captured as image data in a personal computer).

FIG. 5a is a traced micrograph of the collected sample taken under normal light, in which cypris larvae of barnacles were observed at the tip of the arrow. Next, as shown in Fig. 5b, specific light emission was observed in the cypris larvae of the American barnacle upon irradiation with BV excitation light.
As shown in FIG. 5c, image analysis software (NIHI
Cyprus larvae of barnacles were automatically counted by image processing and analysis using mage).

Further, FIG. 6a is a fluorescence emission image (gray scale) in which various barnacles are mixed and observed by irradiating the sample with BV excitation light. For this sample, the image analysis software (NIH
Image processing using Image) was performed to set the density distribution range (in the drawing, the light emitting region is shown as a set of points, that is, in a coarse or dense degree. In the photograph of the reference material, it is displayed in red. ) The pattern of the light emitting region was examined. Then, as shown in FIG. 6c, black-and-white binarization processing was performed, and the Cyprus larva of the red barnacle, which emits light throughout the body, was specifically recognized, and this was automatically counted.

[0061] In the above example, Tatejima barnacles, American barnacles, Red barnacles and Sankaku barnacles were detected. Among them, red barnacles and Sankaku barnacles that strongly adhere to the subsea constructs and are the main causative species of damage were targeted for control. Were determined. The period of high density detection of larvae of the control target species in the adhering stage is from late May to early July and 1
Since it is from mid-January to mid-December, we tried to improve efficiency by intensively performing the above-mentioned standard adhesion control treatment at these times and refraining from such adhesion control treatment at other times. It was confirmed that the adhesion control effect was effective.

[0062]

INDUSTRIAL APPLICABILITY As described above, the present invention allows a computer to recognize the in-vivo fluorescence pattern of barnacle Cyprus larvae in a seawater-containing sample, and immediately determines its type to determine a control target species. As a result, it becomes possible to quickly determine barnacles that are severely damaged in the control area, taking into account changes over time, and as a result,
Applying well-known means to barnacle Cyprus larvae,
It controls the adhesion behavior of specific barnacle Cyprus larvae intensively and efficiently for a predetermined period of time, and surely reduces the seawater utilization efficiency such as increase of seawater resistance and intake pump load, cooling efficiency decrease, blockage of narrow tubes, etc. due to their adhesion. There is an advantage that it can be prevented.

[Brief description of drawings]

FIG. 1 is a side view illustrating the arrangement of organs of a cypris larva of the barnacle.

FIG. 2A is a schematic diagram showing a fluorescence emission pattern of cypris larvae of Tadema barnacles by irradiation with excitation light. FIG. 2B is a schematic diagram showing a fluorescence emission pattern of cypris larvae of American barnacles by irradiation with excitation light. Schematic diagram showing the fluorescence emission pattern of the cypris larvae of the Scutellaria barnacles by (d).

FIG. 3A is a schematic diagram showing a fluorescence emission pattern of a cypris larva of a blue barnacle, which is irradiated by excitation light. FIG. 3B is a schematic diagram showing a fluorescence emission pattern of a cypris larva of a Sarasa barnacle, which is irradiated by excitation light.

FIG. 4 (a) is a schematic diagram showing the fluorescence emission pattern of cypris larvae of the barnacle barnacle upon irradiation with excitation light. FIG. 4 (b) is a schematic diagram showing the fluorescence emission pattern of cypris larvae of the white crocodile barnacle upon irradiation with excitation light. Schematic diagram showing the fluorescence emission pattern of Cyprus larvae of the European barnacle

5A is an explanatory view of various plankton mixed samples observed under a microscope under normal light, and FIG. 5B is an explanatory view showing fluorescence emission of cypris larvae of barnacles observed under a microscope by irradiating with excitation light, and FIG. 5C is an American barnacle. Of computer screen for recognizing and automatically counting cypris larvae

6A is an explanatory view of various barnacle-larvae mixed samples observed under a microscope by irradiating excitation light. FIG. 6B is an explanatory diagram of a computer screen of various barnacle-larvae mixed samples in which concentration distribution ranges are set. Of computer screen for recognizing and automatically counting cypris larvae

[Explanation of symbols]

1 upper 2 first antenna 3 chest 4 cement glands 5 cement pipe 6 oil cells 7 compound eyes 8 chest

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Yosuke Oka             4-33 Komachi, Naka-ku, Hiroshima-shi China Electric Power Stock Association             In-house (72) Inventor Toshiharu Yanagawa             4-33 Komachi, Naka-ku, Hiroshima-shi China Electric Power Stock Association             In-house (72) Inventor Keiji Yamashita             2-75 Tsukura, Shikama-ku, Himeji-shi (72) Inventor Kiyotaka Matsumura             1-223, 1-chome, Usazaki, Shirahama-cho, Himeji-shi (72) Inventor Kyoko Kamiya             32, 32 Higashibori, Shikama-ku, Himeji City (72) Inventor Yoshiko Okada             586 Chiyoda-cho, Himeji City F term (reference) 2B121 AA06 CC21 DA49 EA21 FA13                       FA14                 2G043 AA03 AA04 BA16 CA05 DA02                       EA01 GA07 GB21 JA03 KA02                       KA03 KA05 LA03 NA06

Claims (4)

[Claims] 1. Irradiation of excitation light to adhering stage larvae of barnacles collected from a predetermined amount of seawater, and the fluorescence distribution pattern of each light-emitting individual is input to a computer as digital image information, and this information is recorded as described above. Compared with the in-vivo fluorescence distribution pattern recognition information unique to the species registered in advance in the computer, the species of barnacles matching these fluorescence distribution patterns and the number of individuals per unit volume of the seawater of that species should be controlled. A method for controlling adhesion of barnacles, which comprises determining a species and performing adhesion control treatment for the required period on the species to be controlled. 2. As a pretreatment for the step of irradiating with excitation light,
The method for controlling adherence of barnacles according to claim 1, further comprising a step of fluorescently labeling a sugar chain of a juvenile of barnacles attached with a fluorescently labeled lectin. 3. The excitation light having a wavelength of 400 to 440 nm, which is irradiated to the adhering stage larvae of barnacles, and the emission wavelength of emitted fluorescence is 475 nm or more.
The method for controlling the adhesion of barnacles according to. 4. The barnacles, wherein the barnacles are two or more kinds of barnacles selected from the category of barnacles, including mane barnacles, barnacles, red barnacles, sankaku barnacles, oak barnacles, sala barnacles, barnacles, white barnacles and white barnacles. 1-3
The method for controlling adhesion of barnacles according to any one of 1.