JP2003294739A5 - - Google Patents

Claims (17)

  A step of arranging a probe RNA on the surface of the substrate, a step of hybridizing a biochemical sample to the probe RNA forming a loop structure, a probe RNA or biochemical sample in which a double strand is formed during or after the hybridization, or A method for detecting a biochemical reactant, comprising a step of modifying a label on both of them and a step of detecting and identifying the label.   A step of arranging a probe RNA on the surface of the substrate, a step of hybridizing a biochemical sample whose label is modified in advance to the probe RNA forming a loop structure, and a probe RNA in which a double strand is formed during or after the execution of the hybridization Alternatively, a biochemical reactant detection method comprising a step of modifying a label on a biochemical specimen or both, and a step of detecting and identifying the label.   Detection of biochemical reactants comprising a step of arranging a probe RNA on the surface of a substrate, a step of hybridizing a biochemical sample whose label is modified in advance to a probe RNA forming a loop structure, and a step of detecting and discriminating the label Method.   The probe RNA is arranged on the surface of the substrate, the step of hybridizing the biochemical sample to the probe RNA forming the loop structure, the probe formed with the double strand by the hybridization, and the biochemical sample has a loop structure. A method for detecting a biochemical reactant, comprising a step of detecting and identifying another probe as a difference in height.   The method of modifying the label on the probe RNA and / or biochemical specimen in which double strands are formed is a multistage process comprising two or more stages in which the first label is first modified and the second label is modified with the first label as a target. The method for detecting a biochemical reactant according to any one of claims 1 to 3, wherein the biochemical reactant is modified.   The method for detecting a biochemical reactant according to any one of claims 1 to 3, wherein the label is any one of metal particles (including Si), ceramic particles, fluorescent labels, fluorescent dyes, dyes, and chemical color bodies. .   The method according to any one of claims 1 to 3, wherein the method for detecting / identifying the label is any one of a light scattering method, an SPR spectroscopy method, a chemical coloring method, a fluorescence detection method, an imaging processing method, and a visual method. Method for detecting chemical reactants.   The detection / discrimination method is a method of detecting a probe having a double strand and a biochemical specimen and another probe having a loop structure as a difference in height. An electron or optical microscope observation method, an X-ray microscope The observation method, laser microscope observation method, STM, STS, AFM observation method, electron diffraction method, X-ray diffraction method, X-ray topography method, X-ray surface diffraction method, or fluorescent X-ray analysis method. The method for detecting a biochemical reactant according to claim 4.   The method for detecting a biochemical reactant according to claim 8, wherein the label is larger than the probe RNA.   The method for detecting a biochemical reactant according to claim 6, wherein the metal particles are any one of Au, Al, Ti, Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo, and Si. Ceramic _{particles, SiO 2, TiO 2, ZrO} 2, Al 2 O 3, the detection method of the biochemical reaction of claim 6 MgO is either.   The method for detecting and identifying the label is a visual method, and the label is any one of metal particles (including Si), ceramic particles, pigments, chromosomes, sugar chains, proteins, nucleic acids, enzymes, and cells. The method for detecting a biochemical reactant according to any one of claims 1 to 3.   The method for detecting a biochemical reactant according to claim 12, wherein the particle size of the label is 500 nm or more.   A biochip having a probe RNA arrayed with one end fixed on a substrate, and the arrayed probe RNA having a loop structure so that a main site that binds complementarily to a biochemical sample is located on the substrate side.   A loop structure having a probe RNA arranged with one end fixed on the substrate, and the arranged probe RNA is located on the substrate side at the open end side that is not fixed on the substrate surface or the site where the label can be modified Biochip with.   A loop structure having probe RNA arranged with one end fixed on a substrate, and the arranged probe RNA is located on the substrate side where the first label is modified so that the second label can be modified Biochip with. The biochip according to any one of 請 Motomeko 16 claims 14 substrate material is glass or a semiconductor silicon.