JP2003265136A - Food ingestion based on dna examination - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a food ingestion system and method that is suitable for persons individually. <P>SOLUTION: DNA is tested on a biological individual to determine the health conditions thereof at present or to predict the diagnosis occurring in future. Then, the kinds of and the amounts of foods for maintaining the health and/or for inhibiting the attack of the symptom are selected and ingested. Thus, the ingestion system and method for biological individuals can be established and the dietary prophylaxis and therapy for diseases are made possible. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a system for providing optimal food intake and a food providing method according to the health condition of each living organism and the causative gene found by DNA test.

[0002]

2. Description of the Related Art In recent years, attention has been paid to the relationship between "food" and "health", and it is becoming more and more important to clarify the health condition of each individual and the importance of food intake suitable for it. By the way, freezing technology makes it possible to safely deliver all ingredients to consumers in a form that is closer to nature or in a form that does not impair the nutrients contained in it, and the use of it also from the viewpoint of maintaining health. Further development is expected. Then, a new idea called trans science was advocated, and it was clarified one after another what kind of food was effective for what kind of health and symptoms, and it will be clarified in the future, which will be used as information in the database. Is being accumulated in. On the other hand, the progress in DNA analysis technology is remarkable, such as the determination of the entire sequence of human genes according to the Human Genome Project is also a matter of time. Furthermore, each individual is examined at the gene level through DNA analysis,
It can also reveal their current health status and what illness they may have in the near future.

[0003]

Since the recent research has revealed the symptoms of the disease, it is necessary to optimize the inherently safe food rather than to treat it by ingesting a drug which is notoriously toxic. There is a need for measures to ingest, maintain good health, and even prevent future illness.

[0004]

In view of the above-mentioned problems, living organisms including human beings have their own activities from birth to death due to genes composed of unique DNAs, and the DNAs thereof. The contents are different for each human being, that is, different for each living organism, and therefore the proper diet is also different for each individual, so by using it organically, even higher quality frozen food etc. By providing the best food in
As a diet that suits each individual with food, I found that I can safely and reasonably maintain my health and prevent diseases,
The present invention has been completed.

That is, the present invention (1) conducts a DNA test of an individual organism, determines the health condition of the obtained individual organism, and then evaluates the foods required for maintaining the health of the individual organism. A food intake system based on a DNA test for each individual organism, characterized in that the type or / and the amount of food concerned is selectively provided. (2) A DNA test of an individual organism is carried out, the obtained future symptoms of the individual organism are determined, and then the kind of food or // that is required for preventing the occurrence of the relevant symptoms of the individual organism. And a food intake system based on a DNA test for each individual organism, which selectively supplies the food amount. (3) DNA test of an individual organism is carried out, the health condition of the obtained individual organism is determined, and then the type of food or / and the amount of food required for maintaining the health of the individual organism is selected. A food intake method based on a DNA test for each individual organism, which is provided. (4) A DNA test of an individual organism is performed to determine the resulting future symptoms of the individual organism, and then the type of food or // which is required to prevent the occurrence of the relevant symptoms of the individual organism. And a food intake method based on a DNA test for each individual organism, characterized in that the amount of food concerned is selectively provided.

(5) A DNA test is carried out using DNA collected from an individual organism, and the information on the obtained DNA sequence is compared with the DNA sequence on the database to determine the health condition of the individual organism. , Then determining the type of food or / and the amount of food required to maintain the health of the organism, D for each organism
Food intake system based on NA test. (6) DNA using DNA collected from individual organisms
Necessary for conducting a test, comparing the obtained information on the DNA sequence with the DNA sequence in the database, determining the future symptoms of the organism individual, and then preventing the occurrence of the symptoms of the organism individual. A food intake system based on a DNA test for each individual organism, which is characterized by determining the type of food to be treated and / or the amount of the food. (7) DNA using DNA collected from individual organisms
The type of food required to perform the test, compare the obtained information about the DNA sequence with the DNA sequence on the database, determine the health condition of the organism, and then maintain the health of the organism. Or / and a food intake determination method based on a DNA test for each individual organism, characterized by determining the food amount. (8) DNA using DNA collected from individual organisms
Necessary for conducting a test, comparing the obtained information on the DNA sequence with the DNA sequence in the database, determining the future symptoms of the organism, and then preventing the occurrence of the symptoms in the organism. A food intake determination method based on a DNA test for each individual organism, which comprises determining the type of food to be treated and / or the amount of the food. (9) The DNA test relates to a DNA sequence related to diabetes or arteriosclerosis predisposition, and the food is effective for the health condition of an individual organism against diabetes or arteriosclerosis. The food intake system, the food intake method, or the food intake determination method according to any one of (1) to (8). Is.

[0007] Other objects, characteristics, excellences and advantages of the present invention will be apparent to those skilled in the art from the following description. However, it is to be understood that the description of the present specification including the following description and the specific description shows the preferred embodiments of the present invention and is provided only for the purpose of explanation. Various changes and / or alterations (or modifications) within the spirit and scope of the invention disclosed herein can be made by those of ordinary skill in the art based on knowledge from the following description and other portions of the specification. Would be readily apparent. All patent and literature references cited in this specification are cited for the purpose of illustration and are to be construed as part of this specification, the contents of which are included herein. is there.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail, but it is provided merely for the purpose of explaining the present invention and for reference to specific embodiments thereof. While these descriptions are for explaining specific specific embodiments of the present invention, they limit the scope of the invention disclosed in the present application,
Alternatively, it does not represent a limitation. It should be understood that the present invention is capable of various embodiments based on the ideas of the present specification. Except as otherwise stated in detail, the description of the present specification is carried out or can be carried out by using standard technology, which is well known and conventional to those skilled in the art. Is.

For example, it is becoming clear that lifestyle-related diseases such as hypertension, diabetes, asthma, and allergies, cancer, and various genetic diseases are caused by small mistakes made on the gene. Therefore, by examining the DNA such as the genome of each individual organism such as an individual, it is possible to know the illness that an individual such as an individual is likely to have or the risk of getting a certain illness, and the health condition. Find out what foods you need to attach and what nutrients you need. Especially in the case of lifestyle-related diseases such as high blood pressure and diabetes, foods that should be taken into consideration become clear.

Genetic recombination techniques used for DNA testing of individual organisms are described in, for example, J. Sambrook, EF Fritsch &.
T. Maniatis, "Molecular Cloning: A Laboratory Manu
al (2nd edition) ", Cold Spring Harbor Laboratory Pr
ess, Cold Spring Harbor, New York (1989); DM Glov
er et al. ed., "DNA Cloning", 2nd ed., Vol. 1to 4,
(The Practical Approach Series), IRL Press, Oxfor
d University Press (1995); “Biological Chemistry Laboratory Course 1, Gene Research Method II”, edited by The Biochemical Society of Japan, Tokyo Kagaku Dojin (1)
986); "Biotechnology Chemistry Lecture 2, Nucleic Acid III (Recombinant DNA Technology)", edited by The Japanese Biochemical Society, Tokyo Kagaku Dojin (1)
992); R. Wu ed., "Methods in Enzymology", Vo
l. 68 (Recombinant DNA), Academic Press, New York
(1980); R. Wu et al. Ed., "Methods in Enzymology",
Vol. 100 (Recombinant DNA, Part B) & 101 (Recombin
ant DNA, Part C), Academic Press, New York (1983);
R. Wu et al. Ed., "Methods in Enzymology", Vol. 15
3 (Recombinant DNA, Part D), 154 (Recombinant DNA,
Part E) & 155 (Recombinant DNA, Part F), Academic
Press, New York (1987); JH Miller ed., "Methods
in Enzymology ", Vol. 204, Academic Press, New York
(1991); R. Wu et al. Ed., "Methods in Enzymology",
Vol. 218, Academic Press, New York (1993), etc., or the method described in the literature cited therein, or a method substantially similar to them or a modified method (the description in them can be performed. Are included in the disclosure herein by reference).

In order to obtain an arbitrary region of a nucleic acid such as a gene required for DNA inspection, the polymerase chain reaction (pol
A nucleic acid amplification method such as ymerase chain reaction (PCR) can be used. PCR generally refers to US Pat.
95, etc., eg for enzymatic amplification of a desired nucleotide sequence in vitro. Generally, the PCR method
2 that can preferentially hybridize with template nucleic acid
It includes repeating a cycle for primer extension synthesis using each oligonucleotide primer. Typically, as the primer used in the PCR method, a primer complementary to the nucleotide sequence to be amplified inside the template can be used. Those that are target or are adjacent to the nucleotide sequence to be amplified can be preferably used.

The PCR reaction can be carried out by a method known in the art or a method substantially similar thereto or a modified method, and for example, R. Saiki, et al., Science, 230:
1350,1985; R. Saiki, et al., Science, 239: 487,1988.
HA Erliched., PCR Technology, Stockton Pres
s, 1989; DM Glover et al. ed., "DNA Cloning", 2n
d ed., Vol. 1, (The Practical Approach Series), IR
L Press, Oxford University Press (1995); MA Inn
is et al. ed., "PCR Protocols: a guide tomethods a
nd applications ", Academic Press, New York (1990);
MJ McPherson, P. Quirke and GR Taylor (Ed.),
PCR: a practical approach, IRL Press, Oxford (199
1); MA Frohman et al., Proc. Natl. Acad. Sci. U
It can be carried out according to the method described in SA, 85, 8998-9002 (1988) or the method modified or modified. In addition, the PCR method can be performed using a commercially available kit suitable for it, and can also be performed according to the protocol disclosed by the kit manufacturer or the kit vendor.

In a preferred embodiment, the analysis of the expression profile of a gene is carried out by, for example, SAGE (Serial Analysis of Gene).
Expression) method, microarray, DNA chip, MP
It is executed by SS or the like. Furthermore, data of proteomics such as protein-protein interaction analysis using the two-hybrid system and proteome analysis combining protein two-dimensional electrophoresis and mass spectrum can be appropriately acquired, accumulated in a database, and used in the present invention. It is processed appropriately. Family analysis / linkage analysis and genetic association analysis are also used in the present invention.

In this way, the information on the DNA concerning the individual organism is compared with the information already accumulated, and the genetic basis including the disease predisposition and the like is identified. Since it is effective to perform statistical analysis in identifying such genetic basis, it is advantageous to use a large-scale database system and analysis system linked to a computer system. The nucleic acid sequence database may include, for example, GenBank, EMBL, DDBJ and the like, and the protein sequence database may include, for example, PIR and SwissProt. The usage information includes a gene sequence information analysis database,
It may include a gene expression information analysis database, a gene polymorphism information analysis database, a structural information analysis database, biological system modeling and simulation database, and the like.

Restriction fragment length polymorphism (RFLP: Restriction Fragme
(nt length polymorphism), microsatellite, single nucleotide substitution polymorphism (SNPs: Single Nucleotide Polymorphism), etc. are preferably used in the present invention. In the present invention, from the enormous information including the information on the DNA and genes of each living individual analyzed and elucidated in this way, the one with a high priority is selected based on the connection between them, Food intake is optimized by a computer system equipped with an information processing device having a function of selecting a desired or preferable nutrient, food, and a meal set, and an algorithm suitable for the information processing device. Further, in the present invention, information stored in a database such as experimental data such as proteomics and literature information is effectively used.

In a preferred system of the present invention, for example, when it is found that a DNA is associated with a certain disease as a result of DNA inspection of an individual organism, the relevant gene X1 ,. ． ． , X9
Corresponding to 99 genes of 9, for example, nutrients Y1 ,. ． ． , Y99, there are 99 factors, and it is assumed that they are selected in descending order of priority. So genes X1 ,. ． ． ，
X99 is associated with each of the nutrients Y1 ,. ． ． , Y99, and stored as data together with an appropriate processing program. In this way, various associations are created, and optimal foods, nutrients, and even a meal set satisfying them can be presented as meal candidates for the disease from a database storing associations of various information. In this case, since it becomes difficult to take the same meal every time, it is possible to make a selection in consideration of data such as the type, amount, and frequency of the previous meal. In a typical case, it can be presented as a high priority.

In the present invention, a DNA test of an individual organism, a computer diagnosis of a health condition of the individual organism or a future condition based on the DNA test result, a health maintenance of the individual organism based on the diagnosis, or a prevention of the condition. Also included in the scope of the present invention is a method for selectively providing the kind of food or the amount of food required for that purpose, a DNA probe therefor, and an apparatus which may be combined with a measuring apparatus such as a DNA chip and a DNA array. It Further, the present invention also includes a medium recording a program for controlling the above device.

Diabetes is a group of metabolic diseases caused by a lack of insulin action, and chronic metabolic abnormalities may cause peculiar complications, resulting in various problems in daily life. There is also. The lack of insulin action is due to impaired insulin secretion from β cells of the pancreas, decreased insulin sensitivity or insulin resistance in insulin target tissues.
From these facts, diabetes can now be found by grasping changes in gene expression over time together with the primary genetic predisposition, that is, by analyzing the DNA of each individual.

If it becomes clear that there is a possibility of becoming diabetic, a food item suitable for it is selected.
In the case of diabetes, for example, foods having starch, vegetables, etc.
Foods consisting of fruits, foods consisting of dairy products such as milk and yogurt, foods mainly containing protein such as fish, eggs and tofu, and foods containing sugar etc. that should be paid attention to. In addition, it is required to select appropriate ones from food groups mainly containing oil and fat, combine them, and control their intake.

For example, as a food group having starch,
Examples include cereals, cereals, rice and noodles. For example, the food can be a slice of bread, 1/3 cup of rice, 1/2 cup of cooked cereal or 3/4 cup of dried flaky cereal and 1 small baked potato. You will be instructed to select and ingest one item. The food group consisting of vegetables and fruits includes all edible foods. For example, it is instructed to select and ingest one article from 1/2 cup of tomato juice, 1/2 cup of cooked green peas, 1 cup of raw vegetables or cooked vegetables. Also, for example, 1/2 cup of canned fruit, fruit such as 1 small apple, 1/2 cup of juice such as apple juice, and 3 tablespoons of raisins are selected and ingested. Instructed to do so. Thus, the appropriate food is selected and combined and arranged to ingest it. In particular, because freezing technology enables all ingredients to be safely delivered to consumers in a form that is closer to nature or in a form that does not impair the nutrients contained in it, food and its ingredients are preferably used. You can choose from these. Further, it has been clarified what kind of health and symptoms the food is effective for, and information about it is also accumulated and used in the database of the present invention.

[0021]

EXAMPLES The present invention will be described in detail below with reference to examples, but these examples are provided merely for the purpose of explaining the present invention and for reference to specific modes thereof. .
These exemplifications are intended to illustrate certain specific embodiments of the invention, but are not meant to limit or limit the scope of the invention disclosed herein. It should be understood that the present invention is capable of various embodiments based on the ideas of the present specification. All examples, unless otherwise indicated in detail, are carried out or can be carried out using standard techniques, which are well known and routine to those skilled in the art. ..

Example 1: [Inhibitory effect of human type 2 diabetes model mouse and food]
As an experimental animal, C57BL / KsJ-db / db mice were used to investigate the relationship between diabetes and the suppressive effect of food. Human 2
An experiment was conducted using leptin receptor gene abnormality as a causative gene of type 2 diabetes. Astaxanthin (1 mg / mouse / da), a yellow pigment, was added to 6-week-old db / db mice.
y) was administered for 12 weeks, and body weight, blood glucose, and insulin secretion were evaluated. As a result, regarding the weight after administration for 12 weeks and the effect on blood glucose at any time, there was no change in body weight,
Blood glucose was significantly decreased from time to time.

[0023]

[Table 1]

Blood glucose and insulin levels in the glucose tolerance test were also examined. When blood was collected 2 hours after the glucose tolerance test, insulin secretion was significantly high and blood glucose was also low in the astaxanthin group.

[0025]

[Table 2]

From the above results, astaxanthin administration
These results suggest that hyperglycemia in db / db mice may reduce the glucose toxicity to pancreatic β-cells and may protect insulin secretory function. Thus, food materials containing astaxanthin, such as salmon, salmon roe, white-tailed shrimp, shrimp, crab, Thailand,
It was found that ingesting carp and the like can be expected to have a positive effect on the suppression of diabetes. Similarly, vitamin E,
Ingestion of an antioxidant-containing food containing C improves glucose toxicity to β-cells of the pancreas and is effective in improving symptoms. Moreover, the intake of foods containing probucol improved the dysfunction of pancreatic β-cells due to glucose toxicity.

Example 2 [Inhibitory effect of human type 2 diabetic nephropathy model mouse and food] C57BL / KsJ-db / db mouse was used as an experimental animal, and the relationship between diabetic complication and inhibitory effect of food investigated. An experiment was conducted by taking an abnormality of the leptin receptor gene as a causative gene of human type 2 diabetes. 6 week old db
/ db In mice, the yellow pigment astaxanthin (1 mg
/ mouse / day) for 12 weeks, urinary albumin,
The urinary oxidative stress marker (8-hydroxyguanosine) and the proliferation index of mesangial cells were measured. The above items are known as markers for renal impairment. As a result, it was confirmed that all of the above-mentioned renal impairment markers had improved symptoms.

[0028]

[Table 3]

From the above results, it was suggested that astaxanthin reduced the oxidative stress in the kidney, resulting in the suppression of urinary albumin excretion and the proliferation of mesangial cells. Similarly, intake of antioxidant-containing foods containing vitamins E and C improves glucose toxicity to pancreatic β cells,
Improvement of nephropathy was seen.

Example 3 [Animal Atherosclerosis Experimental Animal Model and Inhibitory Effect by Food] As an experimental animal, apolipoprotein E-deficient mice were used to investigate the relationship between arteriosclerosis and the inhibitory effect by food. The experiment was conducted by taking the apolipoprotein E gene as a gene causing arteriosclerosis. Rosemary component rosmarinic acid (1.52 mg / kg / da) was added to 4-week-old apoE-deficient mice.
Y) was administered for 6 weeks and the effect was observed. As a result, it was evaluated that rosmarinic acid is useful for suppressing the progression of arteriosclerosis.

[0031]

[Table 4]

The above results indicate that the food containing rosmarinic acid is useful for suppressing the progression of arteriosclerosis. Similarly, 1) ginger extract, 2) miso protein, 3) vitamin E + coenzyme Q10, 4) pomegranate juice, and 5) rice tocotrienol were also improved by ingesting foods containing them. Was given. Thus, the DNA test of the individual organism is carried out, and the obtained health condition of the individual organism, for example, mutation D which is a causative gene for diabetes
To determine the NA sequence (typically, leptin receptor gene abnormality, etc.) and the mutated DNA sequence (typically, apolipoprotein E gene, etc.) that is a causative gene for arteriosclerosis, and then to maintain the health of the organism. It is clear that it is possible to provide a food intake system, a food intake method, etc. based on a DNA test for each organism, such as selectively providing the type of food required for the above and / or the amount of the food. Obviously, the present invention can be carried out other than those specifically described in the above description and Examples. Many modifications and variations of the present invention are possible in light of the above teachings, and are therefore within the scope of the claims appended hereto.

[0033]

EFFECTS OF THE INVENTION According to the present invention, it is possible to select an appropriate diet suitable for an individual organism by the DNA test for each individual organism, and for example, it becomes possible to prevent diseases by using food. Also, it will be possible to treat with fun and without side effects. According to the present invention, not only it is possible to provide a meal that suits each individual, but it is possible to prevent and treat diseases by optimal dietary intake.
Therefore, it contributes to food and health, and exerts a remarkably excellent action and effect.

Claims (9)

[Claims] 1. A type of food or / or food required for carrying out a DNA test on an individual organism to determine the health status of the individual organism obtained and then maintaining the health of the individual organism.
And a food intake system based on a DNA test for each individual organism, which selectively supplies the food amount. 2. A DNA test of an individual organism is carried out to determine the resulting future symptoms of said individual organism, and then the type of food or // that is required to prevent said condition of said individual organism. And a food intake system based on a DNA test for each individual organism, which selectively supplies the food amount. 3. A DNA test of an individual organism is carried out, the health condition of the obtained individual organism is determined, and then the type of food or // which is required for maintaining the health of the individual organism.
And a food intake method based on a DNA test for each individual organism, characterized in that the amount of food concerned is selectively provided. 4. A type of food or / or food required to carry out a DNA test on an individual organism, determine the resulting future symptoms of the individual organism, and then prevent said condition of said individual organism. And a food intake method based on a DNA test for each individual organism, characterized in that the amount of food concerned is selectively provided. 5. A DNA test is carried out using DNA collected from an individual organism, and the information on the obtained DNA sequence is compared with the DNA sequence on the database to determine the health condition of the individual organism. Then, the DNA of each individual organism is characterized by determining the type of food or / and the amount of food required for maintaining the health of the individual organism.
Inspection-based food intake system. 6. A DNA test is carried out using DNA collected from an individual organism, and the information about the obtained DNA sequence is compared with the DNA sequence in the database to determine future symptoms of the individual organism. Then, a food intake system based on a DNA test for each individual organism, which comprises determining the type of food or / and the amount of the food required to prevent the symptoms of the individual organism. 7. A DNA test is carried out using DNA collected from an individual organism, and the information on the obtained DNA sequence is compared with the DNA sequence on the database to determine the health condition of the individual organism. Then, the DNA of each individual organism is characterized by determining the type of food or / and the amount of food required for maintaining the health of the individual organism.
Inspection-based food intake decision method. 8. A DNA test is carried out using DNA collected from an individual organism, and the information about the obtained DNA sequence is compared with the DNA sequence in the database to determine future symptoms of the individual organism. Then, a food intake determination method based on a DNA test for each individual organism, which comprises determining the type of food or / and the amount of food required to prevent the symptoms of the individual organism. 9. The DNA test relates to a DNA sequence related to diabetes or arteriosclerosis predisposition, and the food is effective for the health condition of an individual organism against diabetes or arteriosclerosis. The food intake system, the food intake method, or the food intake determination method according to any one of claims 1 to 8.