JP2003246745A - Free radical scavenger containing loquat seed extract as active ingredient - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a useful scavenger which removes free radicals without any side effect. <P>SOLUTION: The free radical scavenger contains a loquat seed extract as an active ingredient. In the free radical scavenger, the loquat seed-derived extract is preferably obtained by grinding a loquat seed, soaking the obtained ground product in at least one solvent chosen from the group consisting of ethanol, methanol, water and hexane and fractionating the supernatant. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a free radical scavenger, and more particularly to a free radical scavenger containing loquat kernel extract.

[0002]

2. Description of the Related Art It is known that extracts having specific components extracted from natural substances such as animals and plants have various effective actions. In particular, among extracts extracted from natural substances such as animals and plants, fish oil extracts and soybean extracts are known to be effective for hyperlipidemia. Natural substances are generally excellent in intestinal absorption, have no side effects, and are highly safe to the human body.

On the other hand, recently, attention has been paid to the fact that lipid peroxides produced by active oxygen produced by phagocytic cells such as neutrophils greatly influence the progress of liver fibrosis. Therefore the inhibition of hepatic fibrosis active oxygen (O ₂ ^-) and is useful administration of a drug with a scavenging ability of free radicals such as hydroxy radicals (· OH), examined various drugs have been made.

It has also been reported that free radicals are greatly involved in the development of arteriosclerosis and ischemic heart disease associated with hypercholesterolemia.

[0005]

However, when extracting a natural substance, it may be difficult to extract the active ingredient well, and it is possible to easily extract the active ingredient and to have various effective actions. The use of natural substances has been desired.

Further, with respect to the scavenger capable of scavenging free radicals harmful to liver fibrosis, arteriosclerosis, ischemic heart disease and the like as described above, satisfactory results have not yet been obtained.

By the way, among the above-mentioned natural substances,
The loquat is usually widely planted as a fruit tree, and although the fruits are used as foods and the leaves are used for skin diseases and the like, the seeds (the loquat kernels) are treated as wastes and are not effectively used. It is not known that loquat seeds (loquat kernels), which is a type of Rosaceae plant commonly planted as fruit trees in Japan, have an effective action superior to that of Kampo extract. Therefore, it is significant if the active ingredient can be extracted from the loquat kernel by some method and a safe health food or beverage can be provided without side effects by utilizing each active ingredient. However, such an effective extract derived from loquat nuclei is hardly known.

[0008] Therefore, an object of the present invention is to provide a useful scavenger capable of scavenging free radicals without side effects.

[0009]

[Means for Solving the Problems] The inventors focused their attention on seed-derived extracts of various plants, and in particular, as a result of examining the free radical scavenging action of loquat kernel extract extracted from loquat kernels, the scavenger of the present invention was identified. I came to find out.

The free-radical scavenger of the present invention is characterized by using the loquat-nucleus-derived extract as an active ingredient. Also,
In a preferred embodiment of the free radical scavenger of the present invention, the loquat-nucleus-derived extract is a pulverized product obtained by pulverizing loquat-nucleus, and is selected from the group consisting of ethanol, methanol, water, hexane, or a mixture thereof. It is characterized in that it is obtained by immersing it in at least one kind of solvent and collecting the supernatant. Further, as a preferred embodiment of the free radical scavenger of the present invention, loquat nucleus-derived extract is linoleic acid, linolenic acid, β-sitosterol, β-sitosterol-3-O-
It is characterized by containing at least one selected from the group consisting of monoglycoside, amygdalin, benzaldehyde, mandenitrile and benzoic acid. A preferred embodiment of the free radical scavenger of the present invention is characterized in that the loquat kernel-derived extract contains β-sitosterol and β-sitosterol-3-O-monoglycoside.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The free radical scavenger of the present invention contains loquat kernel extract. The loquat kernel extract is an extract derived from loquat seeds. The loquat kernel extract to be applied to the present invention includes all loquat kernel extracts as long as they are derived from loquat seeds. Here, the free radical is also called a free radical and means an atomic group having a wide range of unpaired electrons. Generally, molecules having free radicals and substances that generate free radicals are highly reactive and harmful to cells.
Thinking about DNA damages DNA and induces mutations. In the present invention, such free radicals can be efficiently removed.

[0012]

[Method for preparing loquat kernel extract] First, a method for preparing loquat kernel extract will be described with reference to FIG. Figure
FIG. 1 is a diagram showing an example of a method for preparing loquat kernel extract.
The loquat kernel is washed if necessary and dried. It is preferable to perform the drying sufficiently. This is because the subsequent pulverization is performed uniformly.

Next, the loquat nucleus is crushed. The pulverization method is not particularly limited, and a known pulverizer such as a ball mill, a hammer mill, a roller mill, a rod mill, a sample mill, a stamp mill, a day integrator, a mortar, and a blender with a cooling device can be used. Note that a blender with a cooling device is preferable because it is considered that decomposition of the loquat kernel composition and the like may occur due to heat generation during pulverization.

After crushing loquat nuclei to obtain a crushed product, the crushed product is immersed in various solvents. The solvent in this case is not particularly limited, and the solvent can be appropriately set according to the desired effect. The solvent may be a polar or nonpolar solvent such as ethanol, methanol, water, hexane, ethyl acetate, chloroform or acetone. From the viewpoint of obtaining a loquat extract having high cell membrane permeability, methanol, ethanol, water and the like are preferable.

Immersion can be carried out under gentle stirring. The pulverized product is immersed in various solvents to obtain various solutions. Various solutions may be stirred depending on the state of the solution, and the solution may be left as it is depending on the case. When stirring, the stirring can be continued for 5 to 10 days, although not particularly limited.

After that, the supernatant is separated and evaporated to dryness to obtain loquat kernel extract powder. Evaporation to dryness can be performed using an evaporator on a warm bath at 55 ° C to 80 ° C.

[0017]

[Components of loquat kernel extract] The components contained in loquat kernels are extracted by using the solvents with different polarities,
Sorted by its polarity. Therefore, the type and content of the components of loquat kernel extract differ depending on the solvent used.

FIG. 2 shows thin layer chromatograms of loquat kernel extract extracted with various solvents in order to investigate the components of the loquat kernel extract.

According to this thin layer chromatogram, the extract whose solvent is water (hereinafter referred to as "water extract") has a spot only at the origin, and therefore a highly polar compound such as tambata, sugar, amygdalin, etc. Is considered to contain.

In addition, an extract having a solvent of 70% ethanol (hereinafter referred to as 70% ethanol extract) and an extract having a solvent of methanol (hereinafter referred to as methanol extract) have a spot of an origin in a thin layer chromatogram. It is considered to be smaller than water extract and less highly polar compounds such as proteins, sugars and amygdalin.
In 70% ethanol extract and methanol extract, Rf value in the thin-layer chromatogram is 0.63 compound linolenic acid, Rf value 0.53 compound is β-sitosterol, Rf value 0.41 compound is linoleic acid , Rf value
Structural analysis revealed that the compound showing 0.25 was β-sitosterol-3-O-monoglycoside, and at least these compounds were included. In addition, the extract in which the solvent is hexane (hereinafter, hexane extract) was confirmed to be a compound having an Rf value of 0.71 ≧ in a thin layer chromatogram,
It is considered to contain many compounds with low polarity.

[0021]

[Effective amount] The free radical scavenger according to the present invention is prepared in the form of an effective amount of loquat kernel extract and a suitable dosage form.

The dose of loquat kernel extract in the free radical scavenger of the present invention can be changed depending on the disease state and severity of the patient to be administered, the dosage form, the selected administration route and the number of administrations per day. .

The dose of loquat kernel extract in the free radical scavenger of the present invention is at least 37 in rats.
The amount is 5 mg / kg / day, and a lower amount is preferable due to differences in sensitivity in humans.

Further, for administration, oral agents (tablets, capsules, coated tablets, granules, solutions, syrups), suppositories for rectal administration, injections and the like can be used. Patients to be administered often have chronic diseases, need for long-term administration,
From the viewpoint of easy operation, the oral preparation is preferable.

Administration can include other conventional ingredients such as stabilizing preservatives, sweeteners, colorants, fragrances and the like.

[0026]

[Acute toxicity test] Carter. JH et al. Investigated the toxicity of amygdalin, a component of loquat extract, and reported that the lethal dose of amygdalin in rats was 600 mg (Carter. JH Mclefferty, MA, Gold
0an, F., Biochem. Pharmacol. 29,301 (1980)). The lethal dose of loquat extract is its amygdalin content (7.4
%), It can be estimated to be 8060 mg / kg.

[0027]

[Preparation method of loquat kernel extract] As loquat kernels, loquat seeds collected in Kochi Prefecture were sufficiently dried by sun-drying. The loquat kernel extract is crushed (1,000 rpm) with a blender equipped with a cooling device (1,050 g)
Dip in 70% ethanol (2,1000 ml),
The mixture was constantly stirred (300 rpm) with a stirrer, the supernatant was separated 7 days after the immersion, and evaporated to dryness in a warm bath at 70 ° C. with an evaporator to prepare the product. The yield of loquat kernel extract powder was 10.4 g (extraction rate 1.0%).

[0028]

[Ingredient Analysis] In order to study the free radical scavenging action of loquat kernel extract, first, the active ingredients contained in loquat kernel extract were investigated. In other words, prepare loquat kernel extract (Fig. L)
Then, the components were fractionated by using the solvent fractionation method, silica gel column chromatography, molecular sieve column chromatography and thin layer chromatography (TLC) to isolate 8 kinds of components. These compounds are compared with standard products using high performance liquid chromatography (HPLC), TLC, etc., and nuclear magnetic resonance (NMR), mass spectrometry (MS),
The structure was determined by instrumental analysis using infrared absorption spectroscopy (IR). As a result, it was found that the loquat kernel extract contained β-sitosterol, β-sitosterol-3-O-monoglycoside, linoleic acid, linolenic acid, amygdalin, benzaldehyde, mandenitrile and benzoic acid. The identification results are shown below. The measurement conditions and the like are as follows.

[0029]

[Β-Sitosterol] β-sitosterol was isolated from loquat kernel extract, and TLC, EI-MS, 1HNMR (CDCl2), and spectrum data were used for comparative identification. TLC developing solvent _{CHCl3: MeOH: H 2 O (} 8: 2: 0.2) as the Rf value _{0.71 HR-EI-MS C 29} H 30 O, theoretical 414.378, found 414.
387 1HNMR (pyridine-d5) 141.0 (s, lC) 121.9 (d, lC) 10
2.6 (d, 1C), 56.9 (d, 1C), 56.4 (d, lC), 50.5
(D, lC), 46.2 (d, 1C), 42.6 (s, 1C) 40.0 (t, l
C), 39.4 (t, 1C), 37.0 (t, lC), 37.0 (s, 1C),
36.4 (d, 1C) 34.3 (t, lC), 32.24 (t, 1C), 32.15
(D, 1C), 32.15 (d, 1C), 30.3 (t, 1C) 29.6 (d, 1
C), 28.6 (t, lC), 26.6 (t, lC), 24.6 (t, IC),
23.5 (l, 1C), (t, 1C), 20.0 (q, lC), 19.5 (q,
lC), 19.3 (q, 1C), 19.1 (q, iC), 12.2 (q, l
C), 12.0 (q, 1C) IR (KBr, cm-1) 3420

Β-sitosterol-3-O-monoglycoside β-sitostilol-3-O-monoglycoside was isolated from loquat kernel extract, 1 H NMR (σ, CDCl 3) and 13 C-NMR.
The (σ, pyridine-d5) spectrum was identified by comparison with the standard data. 1H NMR (δ, pyridine-d5) 5.6-3.8 (m) 2.8-0.6 (m) 13C-NMR (δ, pyridine-d5) 141.0 (s, 1C), 121.9
(D, 1C), 102.6 (d, lC) 78.6 (d, lC), 78.4 (d, 1
C), 78.3 (d, lC), 75.3 (d, 1C), 71.8 (d, 1C),
62.9 (t, 1C), 56.9 (d, lC), 56.4 (d, 1C), 50.5
(D, 1C), 46.2 (d, lC), 42.6 (s, 1C), 40.0 (t,
lC), 39.4 (t, lC), 37.6 (t, 1C), 37.0 (s, l
C), 6.4 (d, lC), 34.3 (t, 1C), 32.24 (t, 1
C), 32.15 (d, 1C), 30.3 (t, 1C), 29.6 (d, 1
C), 28.6 (t, lC), 26.ru (l, IC), 24.6 (t, l
C), 23.5 <t, 1C), 21.4 (t, lC), 20.0 (q, lC),
19.5 (q, IC), 19.3 (q, 1C), 19.1 (q, 1C), 12.0
(Q, lC), 12.9 (q, 1C)

[0031]

[Benzoic acid] Benzoic acid was isolated from loquat kernel extract and
R, HR-EI-MS, 1H NMR (CDCl3) and HR-EI-MS spectra were compared and identified with the standard data. IR (KBr, cm-1) 3432, 3072, 1687, 1602 1HNMR (δ, CDCl3) 8.15-8.10 (m, 2H) 7.66-7.60
(M. 1H) 7.52-7.46 (m, 2H) 13C-NMR (δ, CDCI3) 171.2
(S, 1C), 133.8 (d, 1C), 130.2 (d, 2C), 129.2
(S, lC), 128.5 ( d, 2C) as _{HR-EI-MS C 7 H} 6 O 2, the theoretical value 122.043, found 12
2.036

[0032]

[Linoleic acid and linolenic acid] Linoleic acid and linolenic acid were identified by comparing HPLC with the loquat kernel extract. In HPLC chromatography of loquat kernel extract, a peak showing the same retention time as the linoleic acid and linolenic acid samples was observed. HPLC measurement condition equipment for linoleic acid and linolenic acid Hitachi D600 type high performance liquid chromatographic detection 240nm column YMC Pack ODS-AQ 150 × 6.6mm ID Measurement temperature 30 ℃ Mobile phase Acetonitrile: Water: Acetic acid (8: 2: 0.1) Flow rate 0.7 ml / min

[Amygdalin, benzaldehyde, and mandenitrile] Amygdalin, benzaldehyde, and mandenitrile were identified by comparing the loquat kernel extract with HPLC and comparing with a standard product. In HPLC chromatography of loquat kernel extract, a peak showing the same retention time as the standard samples of amygdalin, benzaldehyde and mandenitrile was observed.

HPLC measurement conditions for amygdalin Equipment Hitachi D600 type high performance liquid chromatograph Detection UV242nm Column YMC Pack ODS-AQ 150 × 6.6 mm I.D. Measurement temperature 30 ℃ Mobile phase Acetonitrile: Water (1: 4) Flow rate 1 ml / min Retention time: 5.15 minutes

HPLC measurement conditions for benzaldehyde Equipment Hitachi D600 type high performance liquid chromatograph Detection UV280nm Column YMC Pack ODS-AQ 150 × 6.6 mm I.D. Measurement temperature 30 ℃ Mobile phase Acetonitrile: 2% acetic acid (2: 1) Flow rate 1 ml / min Retention time: 4.05 minutes

Mandenitrile HPLC measurement conditions Equipment Hitachi D600 type high performance liquid chromatograph Detection 280nm Column YMC Pack ODS-AQ 150 × 6.6 mm I.D. Measurement temperature 30 ℃ Mobile phase Methanol: Water (7: 3) Flow rate 1 ml / min Retention time: 4.98 minutes

The compounds isolated from loquat kernel extract are shown in Table 1.

[Table 1]

[0038]

EXAMPLES Examples of the present invention will be described below, but the present invention should not be construed as being limited to the following examples.

[0039]

Example 1 First, the effects of loquat kernel extract and its active ingredient on the active oxygen (0 ₂ ⁻ ) scavenging ability were examined. Neutrophils were isolated from rat peripheral blood by gradient centrifugation. On the separated neutrophils, the active oxygen production stimulator fMLP (N-for
Amount of active oxygen produced by administering myl-methionyl-phenylalanine (N-formyl-methionyl-leucyl-phenylalanine)) and various concentrations of extracts or active ingredients
(O ₂ ^-) was measured by cytochrome C reduction method. As a control example, the same test was carried out using commercially available drugs (Radicut and ascorbic acid). The active oxygen scavenging ability is
It was represented by the active oxygen production inhibition rate and active oxygen removal rate obtained by the ratio of the administration group and the non-administration group of loquat kernel extract and its active ingredient. The results are shown in FIGS. 3 (a) to (i). Figure 3
Active oxygen from neutrophils (O ₂ ^-) shows the effect of loquat nuclear extract and its active ingredient on production. The control examples of radicut and ascorbic acid are shown in Fig. 4 (a),
Shown in (b). As a result, the loquat kernel extract and β-sitosterol of the present invention particularly showed excellent active oxygen production inhibitory effect and active oxygen removing effect. On the other hand, Radicut showed a weak effect of suppressing active oxygen production, but did not show an effect of removing active oxygen. Further, ascorbic acid did not show an active oxygen production suppressing effect, but showed a weak active oxygen removing effect.

[0040]

Example 2 Next, fMLP was used as an active oxygen production stimulant.
PMA (Phorbol myristateacetate) was used instead of, and the same test as in Example 1 was carried out. The results are shown in Figures 5 (a) to (i). The control examples of radicat and ascorbic acid are shown in FIGS. 6 (a) and 6 (b), respectively. The loquat kernel extract of the present invention showed an active oxygen production suppressing effect and an active oxygen removing effect.
β-sitosterol was found to have a weak effect of removing active oxygen, but not suppress active oxygen production. on the other hand,
Radicut showed very weak active oxygen production inhibitory effect and active oxygen removing effect, but ascorbic acid did not show the effect.

[0041]

Third Embodiment Furthermore, instead of fMLP, AA (Arachidoni
c Acid (arachidonic acid)) was used and tested in the same manner as in Example 1. The results are shown in FIGS. 7 (a) to (i). The control examples of radicut and ascorbic acid are shown in FIG.
Shown in (a) and (b). The loquat kernel extract of the present invention showed an active oxygen production inhibitory effect when the concentration was increased. Also,
It showed the effect of removing active oxygen. β-sitosterol showed weak active oxygen production inhibitory effect and active oxygen removing effect.
On the other hand, Radicut showed a slight effect of suppressing active oxygen production, and no effect of removing active oxygen was observed. Ascorbic acid showed no effect.

[0042]

EXAMPLE 4 Next, in order to examine active oxygen generator system according xanthine xanthine oxidase, active oxygen loquat nuclear extract using high soluble Tetorarizoumu salt reduction method (O ₂ ^-) was studied scavenging ability. The results are shown in Table 2.

[Table 2] Table 2 shows the SOD activity of loquat kernel extract and active ingredients, radicut, and ascorbic acid. As is clear from Table 2, the loquat kernel extract of the present invention had a high SOD activity of 85% at a concentration of 100 nM, but the active ingredients, radicut and ascorbic acid were around 10%, or not found.

[0043]

Example 5 In addition, a stable radical DPPH (1.1-Diphenyl-2
-picrylhedrazyl (. 1 1- diphenyl-2-Bikuri Le hydrazyl)) free radical "reactive oxygen with (O ₂ ^-)
+ Hydroxy radical (-OH) "scavenging ability was examined. To 50 μM DPPH in ethanol, add loquat kernel extract and active ingredient dissolved in ethanol or distilled water, measure the absorbance at 517 nm within 5 minutes at room temperature, and remove DPPH from loquat kernel extract and each active ingredient. , 50%
The amount of (IC50) elimination was determined and a comparative study was conducted between radicut and ascorbic acid. The results are shown in Table 3.

[Table 3] Table 3 shows loquat kernel extract and its active ingredients, Radicut,
The DPPH 50% elimination amount of ascorbic acid is shown. As is clear from Table 3, the loquat kernel extract of the present invention has a DPPH50
The% erasure amount was as low as 14.1 μM, and radicat and ascorbic acid as controls were similarly recognized as low as 13.7 μM and 28.2 μM.

[0044]

Example 6 Next, the hydroxy radical scavenging ability of loquat kernel extract and each active ingredient was examined using ESR (Electron Spin Resonance). 0.11
1 in 5MPBN (phenyl t-butyl nitrone) phosphate buffer
Hydroxy radicals were generated by adding 0 mM ammonium iron (II) sulfate aqueous solution, and the hydroxy radical scavenging ability of loquat kernel extract and each active ingredient was measured by ESR, and comparative examination with radicut and ascorbic acid was carried out. The results are shown in Table 4.

[Table 4] Table 4 shows loquat kernel extract and its active ingredients, radio cut,
It shows the hydroxy radical scavenging activity of ascorbic acid.
As is clear from Table 4, the scavenging activity of hydroxy radicals by the Fenton reaction (ESR method) is 32
%, Β-sitosterol was 27%, benzoic acid was 24%, and radicat and ascorbic acid as controls showed almost the same scavenging activity as 23% and 27%.

[0045]

The free radical scavenger of the present invention has an advantageous effect that harmful free radicals can be efficiently removed.

Since the free radical scavenger of the present invention is a natural material, it can achieve a desired effect without causing side effects, and is not only responsible for medical treatment but also in the range of utilization of agricultural products. It has an advantageous effect of high social contribution such as expansion and effective use of waste.

[Brief description of drawings]

FIG. 1 is a diagram showing an example of a method for preparing loquat kernel extract.

FIG. 2 is a diagram showing the results of thin layer chromatograms when various solvents were used.

FIG. 3A shows the effects of loquat kernel extract and its effective components on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant fMLP is used.

Figure 3B, when using the active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

FIG. 3C, in the case of using active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

FIG. 3D, when using the active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

FIG. 3E is, when using the active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

Figure 3F] Figure 3F, in the case of using active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

Figure 3G] FIG. 3G, when using the active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

Figure 3H] Figure 3H, when using the active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

Figure 3I] FIG. 3I, in the case of using active oxygen production stimulator fMLP, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its merit components on production (O _2).

FIG. 4A shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulator fMLP was used.

FIG. 4B shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulator fMLP was used.

FIG. 5A shows the effects of loquat kernel extract and its active ingredient on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA is used.

[FIG. 5B] FIG. 5B shows the influence of locus radix extract and its active ingredient on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA is used.

FIG. 5C, when using the active oxygen production stimulator PMA, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its active ingredient on production (O _2).

[FIG. 5D] FIG. 5D shows the influence of the loquat kernel extract and its active ingredient on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA is used.

FIG. 5E, when using the active oxygen production stimulator PMA, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its active ingredient on production (O _2).

Figure 5F] Figure 5F, when using the active oxygen production stimulator PMA, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its active ingredient on production (O _2).

[FIG. 5G] FIG. 5G shows the influence of the loquat kernel extract and its active ingredient on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA is used.

[FIG. 5H] FIG. 5H shows the influence of the loquat kernel extract and its active ingredient on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA is used.

Figure 5I] Figure 5I, when using the active oxygen production stimulator PMA, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its active ingredient on production (O _2).

FIG. 6A shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 6B shows the effects of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7A, when using the active oxygen production stimulator AA, active oxygen from neutrophils ^- shows the effect of loquat nuclear extract and its active ingredient on production (O _2).

FIG. 7B shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7C shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7D shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7E shows the effect of radicat and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7F shows the effect of radicat and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7G shows the effect of radicat and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7H shows the effects of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 7I shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

FIG. 8A shows the effect of radicut and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant AA was used.

FIG. 8B shows the effect of radicat and ascorbic acid on the production of active oxygen (O ₂ ⁻ ) from neutrophils when the active oxygen production stimulant PMA was used.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 31/277 A61K 31/277 31/575 31/575 31/7028 31/7028 31/704 31/704 A61P 39/06 A61P 39/06 F term (reference) 4C086 AA01 AA02 DA08 EA19 EA21 MA03 MA52 ZC37 ZC80 4C088 AB51 AC04 BA08 CA08 MA52 NA14 ZC37 ZC80 4C206 AA01 AA02 CB09 DA04 DA05 DA17 HA13 MA04 MA72 NA14 ZC37 Z80

Claims (4)

[Claims] 1. A free radical scavenger containing an extract derived from loquat kernels as an active ingredient. 2. The loquat kernel-derived extract is obtained by immersing a pulverized product obtained by pulverizing loquat kernels in at least one solvent selected from the group consisting of ethanol, methanol, water, hexane, or a mixture thereof. The free radical scavenger according to claim 1, which is obtained by separating the supernatant. 3. The loquat-derived extract is linoleic acid, linolenic acid, β-sitosterol, β-sitosterol-3-O-
3. The free radical scavenger according to claim 1, containing at least one selected from the group consisting of monoglycoside, amygdalin, benzaldehyde, mandenitrile, and benzoic acid. 4. The free radical scavenger according to any one of claims 1 to 3, wherein the loquat-nucleus-derived extract contains β-sitosterol and β-sitosterol-3-0-monoglycoside. Agent.