JP2003219882A - Gene encoding environmental stress-responsible transcription factor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a gene encoding a stress-responsible transcription factor. <P>SOLUTION: This gene comprises (a) a DNA composed of any base sequence selected from a plurality of specific base sequences, (b) a DNA composed of a base sequence in which one or a plurality of bases are lost, substituted or added in any base sequence selected from a plurality of specific base sequences and encoding an environmental stress-responsible transcription factor and (c) a DNA hybridizing under stringent conditions with DNA composed of any base sequence selected from a plurality of specific base sequences and encoding the environmental stress-responsible transcription factor. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a gene encoding an environmental stress-responsive transcription factor.

[0002]

2. Description of the Related Art A large amount of genomic and cDNA sequences have been determined for several organisms by a gene sequencing project, and a plant model Arabidopsis thaliana (A
rabidopsis thaliana), the complete genomic sequence of two chromosomes has been determined (Lin, X. et al., (1999) Natur.
e 402, 761-768 .; Mayer, K. et al., (1999) Nature 4
02, 769-777.).

The EST (expressed sequence tag) project has also contributed greatly to the discovery of expressed genes (Hofte,
H. et al., (1993) Plant J. 4, 1051-1061 .; Newman,
T. et al., (1994) Plant Physiol. 106, 1241-1255 .;
Cooke, R. et al., (1996) Plant J. 9, 1O1-124. Asam
izu, E. et al., (2000) DNA Res. 7, 175-180.). For example, dbEST (National Center for Biotechnology Inform
ation (NCBI) EST database) contains partial cDNA sequences, and more than half of all genes (ie, approximately 28,000 genes) are reproduced (completely sequenced gene of chromosome 2 of Arabidopsis thaliana). Estimated from content [Lin, X. et
al., (1999) Nature 402, 761-768.]).

In recent years, microarray (DNA chip) technology has become a useful tool for analyzing gene expression on the genome scale (Schena, M. et al., (1995) Science 2).
70,467-470 .; Eisen, MB and Brown, PO (1999)
Methods Enzymol. 303, 179-205.). The technology using this DNA chip is to arrange cDNA sequences on a glass slide at a density of 1,000 genes / cm2 or more. The cDNA sequences arranged in this way can be used for RNAs of different cell types or tissue types.
Simultaneous hybridization with a pair of two-color fluorescence-labeled cDNA probes prepared from a sample enables direct and large-scale comparative analysis of gene expression. This technology
Initially demonstrated by analyzing 48 Arabidopsis genes for differential expression in roots and shoots (Schena, M. et al., (1995) Science 270, 467-
470.). In addition, microarrays have been used to probe 1,000 randomly picked clones from a human cDNA library to identify novel genes that respond to heat shock and protein kinase C activation.
(Schena, M. et al., (1996) Proc. Natl. Acad. Sci.
USA, 93, 10614-10619.).

On the other hand, the expression profile of inflammatory disease-related genes under various induction conditions has been analyzed by the method using this DNA chip (Heller, RA.
et al., (1997) Proc. Natl. Acad. Sci. USA, 94, 215
0-2155.). In addition, microarrays have been used to analyze the dynamic expression of the yeast genome, which consists of more than 6,000 coding sequences (DeRisi, JL et al., (199
7) Science 278, 680-686 .; Wodicka, L. et al., (199
7) Nature Biotechnol. 15, 1359-1367.).

However, in the field of plants, there have been only a few reports on microarray analysis (Schena, M. et al., (1995) Science 270, 467-47.
0.; Ruan, Y. et al., (1998) Plant J. 15, 821-833 .;
Aharoni. A. et al., (2000) Plant Cell 12, 647-66
1 .; Reymond, P. et al., (2000) Plant Cell 12, 707-
719.).

The growth of plants is significantly affected by environmental stress such as drought, high salt concentration and low temperature. Of these stresses, drought or water deficit are the most severe limiting factors for plant growth and crop production. Drought stress causes various biochemical and physiological responses in plants.

Plants acquire responsiveness and adaptability to stress in order to survive under these stress conditions. Recently, several genes that respond to drought at the transcriptional level have been described (Bohnert, HJ et al., (1995) Plant.
Cell 7, 1099-1111 .; Ingram, J., and Bartels, D. (1
996) Plant Mol. Biol. 47, 377-403 .; Bray, EA (19
97) Trends Plant Sci. 2, 48-54 .; Shinozaki. K., an
d Yamaguchi-Shinozaki, K. (1997) Plant Physiol. 11
5, 327-334 .; Shinozaki, K., and Yamaguchi-Shinoz
aki, K. (1999). Molecular responses to drought str
ess. Molecular responses to cold, drought, heat an
d salt stress in higher plants.Edited by Shinozak
i, K. and Yamaguchi-Shinozaki, KRG Landes Com
pany. ； Shinozaki, K., and Yamaguchi-Shinozaki, K.
(2000) Curr. Opin. Plant Biol. 3, 217-223.).

On the other hand, stress-inducible genes have been used to improve stress tolerance of plants by gene transfer (Holmberg, N., and Bulow, L. (1998) Tren.
ds Plant Sci. 3, 61-66 .; Bajaj, S. et al., (1999)
Mol. Breed. 5, 493-503.). It is important to analyze the function of stress-inducible genes not only for further elucidating the molecular mechanism of stress tolerance and stress response of higher plants but also for improving stress tolerance of crops by genetic engineering.

DRE / CRT (drought responsive element / C-repeat sequence) is a dry, high salinity and low temperature stress responsive gene ABA (abscisic acid: a kind of plant hormone) which is a signal transduction of seed dormancy and environmental stress. Functioning as a factor.) Has been identified as an important cis-acting element in expression independent of (Yamaguchi-Shinozaki,
K., and Shinozaki, K. (1994) Plant Cell 6, 251-26
4 .; Thomashow, MF et al., (1999) Plant Mol. Biol.
50, 571-599 .; Shinozaki, K., and Yamaguchi-Shinoz
aki, K. (2000) Curr. Opin. Plant Biol. 3, 217-22
3.). A transcription factor (DREB / CBF) involved in DRE / CRT-responsive gene expression has been cloned (Stockinger.
EJ et al., (1997) Proc. Natl. Acad. Sci. USA 94,
1035-1040 .; Liu, Q. et al., (1998) Plant Cell 10,
1391-1406 .; Shinwari, ZK et al., (1998) Bioche
m. Biophys. Res. Commun. 250, 161-170 .; Gilmour,
SJ et al., (1998) Plant J. 16, 433-443.). DREB1 / CB
F is thought to function in cold-responsive gene expression, and DREB2 is involved in drought-responsive gene expression. Strong tolerance to freezing stress has been observed in transgenic Arabidopsis plants overexpressing the CBF1 (DREB1B) cDNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter (Jaglo-Ottosen, KR e
t al., (1998) Science 280, 104-106.).

[0011] The present inventors have shown that overexpression of the DREB1A (CBF3) cDNA in transgenic plants under the control of the CaMV 35S promoter or the stress-inducible rd29A promoter results in strong constitutive expression of the stress-inducible DREB1A target gene. And increased resistance to freezing, drought and salt stress (Liu, Q. et al., (1998) Plant Cell 10, 1391-14).
06 .; Kasuga, M. et al., (1999) Nature Biotechnol.
17, 287-291.). In addition, the present inventors have already found that rd29A / lti78
/ cor78, kin1, kin2 / cor6.6, cor15a, rd17 / cor47 and e
Six DREB1A target genes such as rd10 have been identified (Kasug
a, M. et al., (1999) Nature Biotechnol. 17, 287-29
1.). However, D in transgenic plants
It is not fully understood how overexpression of the REB1A cDNA enhances stress resistance to freezing, desiccation and salt. To study the molecular mechanism of drought and freeze resistance, it is important to identify and analyze more genes regulated by DREB1A.

[0012]

By the way, DREB1A is known as a transcription factor showing responsiveness to environmental stress (Japanese Patent Laid-Open No. 2000-60558). Regarding DREB1A, by overexpressing the DREB1A gene in a plant, the environmental stress tolerance of the plant can be increased. However, as of now, not many transcription factors have been known as transcription factors capable of increasing environmental stress tolerance of plants.

Therefore, an object of the present invention is to provide a gene encoding an environmental stress responsive transcription factor.

[0014]

Means for Solving the Problems As a result of intensive research to solve the above problems, the present inventor has applied cDNA microarray analysis and is specific to environmental stress such as low temperature stress, drought stress and salt stress. The present inventors have succeeded in identifying a gene encoding a transcription factor exhibiting responsiveness to, and completed the present invention.

That is, the present invention includes the following.

(1) A gene encoding an environmental stress-responsive transcription factor containing the following amino acid sequence (a) or (b): (a) Any amino acid sequence selected from SEQ ID NO: 2n (n = 1 to 36) (b) One or more amino acid sequences selected from SEQ ID NO: 2n (n = 1 to 36) Consisting of an amino acid sequence in which the amino acid of is deleted, substituted or added,
Amino acid sequence showing responsiveness to environmental stress and having transcription factor activity

(2) The gene according to (1), which comprises the following DNA (a), (b) or (c): (A) DNA consisting of any base sequence selected from SEQ ID NO: 2n-1 (n = 1 to 36) (b) Any selected from SEQ ID NO: 2n-1 (n = 1 to 36) DNA consisting of a nucleotide sequence in which one or more nucleotides have been deleted, substituted or added in the nucleotide sequence of, and coding for an environmental stress responsive transcription factor (c) SEQ ID NO: 2n-1 (n = an integer from 1 to 36) DNA that hybridizes with a DNA consisting of any one of the nucleotide sequences selected from the following under stringent conditions and encodes an environmental stress-responsive transcription factor

(3) The gene according to (1) or (2), wherein the environmental stress is at least one selected from the group consisting of low temperature stress, drought stress and salt stress. (4) An expression vector containing the gene according to any one of (1) and (2). (5) A transformant containing the expression vector according to (4). (6) A transgenic plant containing the expression vector according to (4). (7) The transgenic plant according to (6), wherein the plant is a plant body, a plant organ, a plant tissue or a plant cultured cell. (8) A method for producing a stress-tolerant plant, which comprises culturing or cultivating the transgenic plant according to (6) or (7).

[0019]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below.

The inventors of the present invention conducted a biotinylated CAP trapper method (Carninci. P. et al., (1996) Genomics, 37, 3) from plants having different conditions such as dry treated plants and low temperature treated plants.
27-336.) And constructed a full-length cDNA library of Arabidopsis thaliana (Seki. M. et al., (1998) Plant J. 15,
707-720.), And about 7,000 full-length cDNAs containing a stress-inducible gene were used to prepare full-length cDNA microarrays of Arabidopsis thaliana. In addition to these drought- and cold-induced full-length cDNAs, a cDNA microarray was prepared using the gene targeted by DREB1A, which is a transcriptional regulator that controls the expression of stress-responsive genes. Then, the expression patterns of genes under drought stress and low temperature stress were monitored, and stress-responsive genes were comprehensively analyzed. As a result, 277 drought-inducible genes, 53 cold-inducible genes and 194 high salt stress-inducible genes were isolated from a cDNA microarray containing about 7,000 full-length cDNAs.

Then, the nucleic acid base sequences of these environmental stress responsive genes converted into amino acid sequences are converted into GenBan.
For amino acid sequence Data registered in k Database
Those that code for transcription factors by searching for blast X (genes encoding known transcription factors and E-value: e
It was successful in identifying homologues with a score higher than ^-10 ). In the following description, the transcription factor identified in the present invention may be referred to as an environmental stress-responsive transcription factor.

As described above, the full-length cDNA microarray is an effective tool for analysis of the expression pattern of Arabidopsis thaliana drought / low temperature stress-inducible genes and analysis of target genes of stress-related transcriptional regulatory factors.

1. Isolation of Transcription Factor A transcription factor has a function of binding to a cis element existing upstream of a gene and activating transcription of the gene downstream thereof. The transcription factor isolated in the present invention is induced by environmental stress such as low temperature, drought and high salt concentration.

Environmental stress-responsive transcription factors include those belonging to the DREB family, those belonging to the ERF family, those belonging to the zinc finger family, those belonging to the WRKY family, those belonging to the MYB family, those belonging to the bHLH family, and the NAC family. Belonging to
They are roughly classified into those belonging to the homeodomain family and those belonging to the bZIP family.

In isolating the transcription factor, first, a stress-responsive gene is isolated using a microarray.
Microarrays include Arabidopsis
s) full-length cDNA library, RD (Responsive to Dehydration) gene, ERD (Ea
rly Responsive to Dehydration) gene, a PCR amplified fragment obtained from λ control template DNA fragment (TX803, manufactured by Takara Shuzo Co., Ltd.) as an internal standard, and a mouse nicotinic acid acetylcholine receptor epsilon subunit (nAChRE) gene as a negative control and A total of about 7,000 cDNAs consisting of the homologous gene of mouse glucocorticoid receptor can be used.

The plasmid DNA extracted using the Kurabo plasmid preparation device was used for sequence analysis, and a DNA sequencer (ABI PRISM 3700. PE Applied Biosystems, C
A, USA) to determine the sequence. Based on the GenBank / EMBL database, sequence homology search is performed using the BLAST program.

Next, after poly A selection, a reverse transcription reaction is carried out to synthesize double-stranded DNA, and the cDNA is inserted into a vector.

The cDNA inserted into the vector for preparing the cDNA library is amplified by the PCR method using primers complementary to the sequences of the vector on both sides of the cDNA. Examples of the vector include λZAPII and λPS.

The microarray can be produced by a usual method and is not particularly limited. For example, gene tip microarray stamp machine GTMASS SYS
Using TEM (Nippon Laser & Electronics Lab.),
The PCR product is loaded from a microtiter plate and spotted on a microslide glass at regular intervals. The slide is then dipped in blocking solution to prevent the expression of non-specific signals.

Examples of plant materials include wild-type and disrupted strains of specific genes, and transgenic plants into which the cDNA of DREB1A has been introduced can be used. The plant species include Arabidopsis thaliana, tobacco, rice, etc., but Arabidopsis thaliana is preferable.

Drying and low temperature stress treatment can be carried out by known methods (Yamaguchi-Shinozaki, K., and
Shinozaki, K. (1994) Plant Cell 6, 251-264.).

After the stress treatment, the plants (wild type and DREB1A overexpressing transformants) are sampled and frozen and stored in liquid nitrogen. Wild type and DREB1A
Overexpressing transformants are used in experiments to identify the target gene for DREB1A. MRNA is isolated and purified from the plant using a known method or kit.

Cy3 dUTP or Cy5 dUTP (Amersham P for labeling)
harmacia) and perform reverse transcription of each mRNA sample and use for hybridization.

After hybridization, the microarray is scanned using a scanning laser microscope or the like. Imagen is a program for microarray data analysis.
e Ver 2.0 (BioDiscovery) and QuantArray (GSI Lumonic
s) etc. can be used.

After the scan, the gene is isolated by preparing a plasmid having the gene of interest.

To determine the transcription factor, the nucleotide sequence of the above-mentioned gene is analyzed and the database (GenBank / EMBL, AB
It is performed using a gene analysis program based on the genome information of (RC). The isolated genes can be classified into those having both properties of drought stress inducibility and cold stress inducibility, those specific to drought stress inducibility, and those specific to cold stress inducibility. According to the gene analysis program, genes encoding 36 kinds of transcription factors are identified from the above genes. The nucleotide sequences of genes encoding these 36 types of transcription factors are shown in SEQ ID NO: 2n.
-1 (n is an integer from 1 to 36) and the amino acid sequence of the transcription factor is shown in SEQ ID NO: 2n (n is an integer from 1 to 36). Table 1 shows the sequence numbers and the gene names of genes encoding transcription factors.

[0037]

[Table 1]

However, as long as the transcription factor of the present invention functions as an environmental stress responsive transcription factor, one or a plurality of nucleotide sequences selected from SEQ ID NO: 2n-1 (n is an integer of 1 to 36), Preferably one or several (eg 1 to
(10, 1 to 5) bases may be deleted, substituted or added. Further, SEQ ID NO: 2n-1
(N is an integer of 1 to 36) A DNA that hybridizes with a DNA consisting of any nucleotide sequence selected from (n is an integer of 1 to 36) under stringent conditions and that encodes an environmental stress-responsive transcription factor is also a transcription factor of the present invention. included. Here, the stringent condition means that the sodium concentration is 25 to 500 m.
M, preferably 25 to 300 mM, and the temperature is 42 to 68 ° C, preferably 42 to 65 ° C. More specifically, 5 x SSC (83 mM
NaCl, 83 mM sodium citrate), temperature 42 ° C.

The 36 kinds of transcription factors isolated in the present invention can be classified as follows. DREB family: RAFL05-11-M11, RAFL06-11-K21, RA
FL05-16-H23, RAFL08-16-D16 ERF family: RAFL08-16-G17, RAFL06-08-H20 Zinc finger family: RAFL07-10-G04, RAFL0
4-17-D16, RAFL05-19-M20, RAFL08-11-M13, RAFL04-15-
K19, RAFL05-11-L01, RAFL05-14-C11, RAFL05-19-G24,
RAFL05-20-N02 WRKY Family: RAFL05-18-H12, RAFL05-19-E19, RA
FL06-10-D22, RAFL06-12-M01 MYB family: RAFL05-14-D24, RAFL05-20-N17, RAF
L04-17-F21 bHLH Family: RAFL09-12-N16 NAC Family: RAFL05-19-I05, RAFL05-21-I22, RAF
L08-11-H20, RAFL05-21-C17, RAFL05-08-D06 Homeodomain family: RAFL05-20-M16, RAFL11-
01-J18, RAFL11-09-C20 bZIP family: RAFL05-18-N16, RAFL11-10-D10, RA
FL04-17-N22, RAFL05-09-G15 RAFL05-21-L12 cannot be classified as above.

Once the nucleotide sequence of the gene encoding the transcription factor of the present invention has been determined, thereafter, PCR is carried out by chemical synthesis or by using the cloned probe as a template.
Alternatively, or by hybridizing with a DNA fragment having the nucleotide sequence as a probe, the gene encoding the transcription factor of the present invention can be obtained. Furthermore, a mutant form of the gene encoding the transcription factor of the present invention, which has the same function as the gene encoding the transcription factor before mutation, can also be synthesized by a site-directed mutagenesis method or the like.

In order to introduce a mutation into the nucleotide sequence of a gene encoding a transcription factor, the Kunkel method, Gapped duplex
A known method such as a method or a method similar thereto can be adopted. For example, a mutagenesis kit using site-directed mutagenesis (eg Mutant-K (TAKARA) or Mut
ant-G (manufactured by TAKARA), etc., or TAKARA
Mutations are introduced using the LA PCR in vitro Mutagenesis series kit of the same company.

Here, "environmental stress" generally means abiotic stress, for example, drought stress, low temperature stress, high salt concentration stress, strong light stress and the like.
"Dry" means a state of lack of water, and "low temperature" means a state of being exposed to a temperature lower than the optimum living temperature of each species (for example, in the case of Arabidopsis thaliana-20 to + 21 ° C).
The temperature is continuously exposed for 1 hour to several weeks. Further, the "high salt concentration" means a state when NaCl having a concentration of 50 mM to 600 mM is continuously treated for 0.5 hours to several weeks. "Strong light stress" means a state in which the plant is exposed to strong light exceeding the photosynthetic capacity, for example, 5,000 to 10,000 Lx.
This is the case when the above light is applied. One kind of these environmental stresses may be loaded, or a plurality of kinds of environmental stresses may be loaded.

2. Construction of Expression Vector The expression vector of the present invention can be obtained by ligating (inserting) the gene encoding the transcription factor of the present invention into an appropriate vector. The vector for inserting the gene encoding the transcription factor of the present invention is not particularly limited as long as it can be replicated in the host, and examples thereof include a plasmid, a shuttle vector, a helper plasmid and the like.

As plasmid DNA, Escherichia coli-derived plasmids (for example, pBR322, pBR325, pUC118, pUC119, pU
C18, pUC19, pBluescript etc.), plasmids derived from Bacillus subtilis (eg pUB110, pTP5 etc.), yeast derived plasmids (eg YEp13, YCp50 etc.), etc., and phage DNA
As λ phage (Charon4A, Charon21A, EMBL3, EM
BL4, λgt10, λgt11, λZAP, etc.). Furthermore, animal viruses such as retrovirus or vaccinia virus, and insect virus vectors such as baculovirus can also be used.

To insert the gene encoding the transcription factor of the present invention into a vector, first, the purified DNA is cleaved with an appropriate restriction enzyme and inserted into the restriction enzyme site or the multiple cloning site of an appropriate vector DNA. And a method of ligating to a vector.

The gene encoding the transcription factor of the present invention is
When a reporter gene, for example, the GUS gene widely used in plants is linked to the 3'end and used, the expression intensity of the gene can be easily evaluated by examining the GUS activity. As a reporter gene, GUS
In addition to the gene, luciferase, green fluorescein protein and the like can be used.

3. Preparation of transformant The transformant of the present invention can be obtained by introducing the expression vector of the present invention into a host. Here, the host is not particularly limited as long as it can express the gene encoding the transcription factor of the present invention, but plants are preferable. When the host is a plant, a transformed plant (transgenic plant) can be obtained as follows.

Plants to be transformed in the present invention include whole plants, plant organs (eg leaves, petals, stems, roots,
Seeds, etc., plant tissue (e.g. epidermis, phloem, soft tissue, xylem,
Vascular bundle etc.) or plant cultured cells. Examples of plants used for transformation include plants belonging to Brassicaceae, Gramineae, Solanaceae, and Legumes (see below), but are not limited to these plants.

Brassicaceae: Arabidopsis thaliana Solanaceae: Tobacco (Nicotiana tabacum) Gramineae: Maize (Zea mays), Rice (Oryza sativ)
a) Legumes: Soybean (Glycine max) The above recombinant vector is introduced into a plant by a usual transformation method such as electroporation (electroporation), Agrobacterium method, particle gun method, PEG method and the like. can do.

For example, when the electroporation method is used, the voltage is 500 to 1600 V, 25 to 1000 μF, 2 by an electroporation apparatus equipped with a pulse controller.
The gene is introduced into the host by treatment under the condition of 0 to 30 msec.

When the particle gun method is used, the plant body, plant organ, or plant tissue itself may be used as it is, or may be used after preparing a slice, or may be used after preparing a protoplast. Good. The sample thus prepared was used for the gene transfer device (for example, PDS-1000 /
He, etc.). Although the treatment conditions vary depending on the plant or sample, the treatment is usually performed at a pressure of about 1000 to 1800 psi and a distance of about 5 to 6 cm.

By using a plant virus as a vector, the target gene can be introduced into a plant. Available plant viruses include, for example,
The cauliflower mosaic virus can be mentioned. That is, first, a viral genome is inserted into a vector derived from Escherichia coli or the like to prepare a recombinant, and then these target genes are inserted into the viral genome. The target gene can be introduced into the plant host by cutting out the thus modified viral genome from the recombinant with a restriction enzyme and inoculating the plant host.

In the method using the Ti plasmid of Agrobacterium, Agrobacterium (Agrobacteri
When a bacterium belonging to the genus (um) genus infects a plant, the target gene is introduced into a plant host by utilizing the property that a part of the plasmid DNA of the bacterium belongs to the plant genome.
Among bacteria belonging to the genus Agrobacterium (Agrobacterium tumefacien)
s) infects plants to form tumors called crown gall, and also causes Agrobacterium rhizogenes (Agrob
acteriumu rhizogenes) infects plants to generate hairy roots. These are that the T-DNA region (Transferred DNA) on the plasmid present in each bacterium called Ti plasmid or Ri plasmid upon infection is transferred into the plant and integrated into the plant genome. It is due to.

In the T-DNA region on the Ti or Ri plasmid,
By inserting the DNA to be incorporated into the plant genome, the desired DNA can be incorporated into the plant genome when a bacterium of the genus Agrobacterium infects the plant host.

The tumor tissue, shoots, hairy roots and the like obtained as a result of the transformation can be used as they are for cell culture, tissue culture or organ culture, or by using a conventionally known plant tissue culture method. The plant can be regenerated by administering a suitable concentration of a plant hormone (auxin, cytokinin, gibberellin, abscisic acid, ethylene, brassinolide, etc.).

The vector of the present invention is not only introduced into the above-mentioned plant host, but also the genus Escherichia such as Escherichia coli, Bacillus subtilis.
s) or Pseud, or Pseudomonas putida
Saccharomyces cerevisia, a bacterium belonging to the genus Pseudomonas such as omonas putida).
e), Schizosaccharomyces
It is also possible to obtain transformants by introducing into yeast such as pombe), animal cells such as COS cells and CHO cells, or insect cells such as Sf9. When a bacterium such as Escherichia coli or yeast is used as a host, the recombinant vector of the present invention is capable of autonomous replication in the bacterium, and at the same time, a gene encoding the transcription factor of the present invention and a ribosome located upstream of the gene. It is preferably composed of a binding sequence, a promoter sequence, and a transcription termination sequence located downstream of the gene.

The method for introducing the recombinant vector into the bacterium is not particularly limited as long as it is a method for introducing the DNA into the bacterium. Examples thereof include a method using calcium ions and an electroporation method.

When yeast is used as a host, for example, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Are used. The method for introducing the recombinant vector into yeast is not particularly limited as long as it is a method for introducing DNA into yeast, and examples thereof include an electroporation method, a spheroplast method, and a lithium acetate method.

When an animal cell is used as a host, monkey cell CO
S-7, Vero, Chinese hamster ovary cells (CHO cells), mouse L cells, etc. are used. Examples of the method for introducing the recombinant vector into animal cells include the electroporation method, calcium phosphate method, lipofection method and the like.

When insect cells are used as hosts, Sf9 cells and the like are used. Examples of the method for introducing the recombinant vector into insect cells include the calcium phosphate method, lipofection method, electroporation method and the like.

Whether or not the gene has been incorporated into the host can be confirmed by the PCR method, Southern hybridization method, Northern hybridization method or the like. For example, DNA is prepared from the transformant, a DNA-specific primer is designed, and PCR is performed. PCR is performed under the same conditions as those used for preparing the plasmid. After that, agarose gel electrophoresis about the amplification product, polyacrylamide gel electrophoresis or capillary electrophoresis, etc., stained with ethidium bromide, SYBR Green solution, and by detecting the amplification product as one band, Confirm that it has been transformed. Also, use a primer that has been labeled with a fluorescent dye in advance.
Amplification products can also be detected by performing PCR. Furthermore, by binding the amplification product to a solid phase such as a microplate,
A method of confirming the amplification product by fluorescence or enzymatic reaction may also be adopted.

4. Plant Production In the present invention, transformed plant cells can be regenerated from the transformed plant cells or the like. As a playback method,
A method in which callus-like transformed cells are transferred to a medium in which the type and concentration of hormone are changed and cultured to form adventitious embryos to obtain a complete plant is adopted. The medium used is LS
Examples of the medium include MS medium.

The "method for producing a plant" of the present invention comprises the step of introducing a plant expression vector into which a gene encoding the above environmental stress-responsive transcription factor is inserted into a host cell to obtain a transformed plant cell, The method includes the steps of regenerating a transformed plant from transformed plant cells, obtaining a plant seed from the obtained transformed plant, and producing a plant from the plant seed.

To obtain plant seeds from transformed plants,
For example, a transformed plant is collected from a rooting medium, transplanted into a pot containing water-containing soil, grown at a constant temperature to form flowers, and finally seeds. Also,
To produce a plant from a seed, for example, when the seed formed on the transformed plant matures, it is isolated,
Plants are produced by sowing in soil containing water and growing under constant temperature and illuminance. The plant bred in this way becomes an environmental stress resistant plant corresponding to the stress responsiveness of the introduced gene encoding the environmental stress responsive transcription factor.

[0065]

EXAMPLES The present invention will be described in more detail below with reference to examples. However, the technical scope of the present invention is not limited to these examples.

Example 1 Isolation of Gene Encoding Environmental Stress-Responsive Transcription Factors 1. Materials and methods (1) Arabidopsis cDNA clone In addition to the gene isolated from the full-length cDNA library of Arabidopsis, RD (Responsive to Dehydration) gene, ERD (Early Responsive to Dehydration) gene,
Kin1 gene, kin2 gene, cor15a gene, λ control template DNA amplified by PCR as an internal standard, and mouse nicotinic acid acetylcholine receptor epsilon subunit (nAChRE) gene and mouse glucocorticoid receptor homology as negative controls. A total of about 7,000 cDNAs of sex genes were used for microarray preparation. Positive control: Drought-inducing gene (dehydration responsiveness: rd, and initial dehydration responsive gene: erd) Internal standard: λ Control template DNA amplified by PCR (TX803, manufactured by Takara Shuzo, hereinafter referred to as "control fragment") Negative Controls: Nicotinic acetylcholine receptor epsilon subunit (nAChRE) gene and mouse glucocorticoid receptor homolog gene with virtually no homology to any sequence in the Arabidopsis database to assess nonspecific hybridization.

(2) Using the Arabidopsis full-length cDNA microarray biotinylated CAP trapper method, the present inventor
From the plants of bidopsis, a full-length cDNA library was constructed under different conditions (for example, dry treatment, low temperature treatment and untreated at various growth stages from germination to mature seeds).
From the full-length cDNA library, the present inventor isolated about 7,000 independent Arabidopsis full-length cDNAs, respectively.
According to a known method (Eisen and Brown, 1999), the cDNA fragments amplified by PCR were aligned on a slide glass. The present inventor has established that about 7,000 Arabidop containing the following genes:
A full-length cDNA microarray containing sis full-length cDNA was prepared.

(3) Drying-inducible gene, low temperature-inducible gene and high salt concentration-inducible gene using cDNA microarray In this example, a full-length cDNA microarray containing about 7,000 full-length Arabidopsis full-length cDNAs was used for drying. Inducible genes, low temperature inducible genes and high salt concentration inducible genes were isolated.

Cy3 and Cy5 fluorescently labeled probes of the various stressed and unstressed plants described above were mixed and hybridized with a full-length cDNA microarray containing about 7,000 full-length Arabidopsis full-length cDNAs. One mRNA sample was labeled with Cy3-dUTP and the other mRNA
Dual labeling of a pair of cDNA probes that label NA samples with Cy5-dUTP enables simultaneous hybridization to DNA elements on a microarray, allowing gene expression between two different conditions (ie, with or without stress). Facilitates direct quantitative measurement of. The hybridized microarray was scanned by two separate laser channels for Cy3 and Cy5 emission from each DNA element. Then 2 of each DNA element
The intensity ratio of the two fluorescent signals was measured as a relative value, and the change in the differential expression of the genes represented by the cDNA spots on the microarray was determined. In this example, the α-tubulin gene, whose expression levels were almost equivalent under the two experimental conditions to be analyzed, was used as an internal control gene.

A procedure for identifying drought-inducible genes, cold-inducible genes and high salt concentration-inducible genes in a full-length cDNA microarray containing about 7,000 full-length Arabidopsis cDNAs is shown.

One of the above-mentioned stress-derived plant-derived mRNAs and unstressed wild-type plant-derived mRNAs were used for the preparation of Cy3-labeled cDNA probes and Cy5-labeled cDNA probes, respectively. . These cDNA probes were mixed and hybridized with a cDNA microarray. In this example, a control fragment whose expression level was almost the same under the two conditions was used as an internal control gene. A gene having an expression ratio (dry / stress-free, low temperature / stress-free, or high salt concentration / stress-free) exceeding 5 times that of the control fragment was defined as a gene induced by the applied stress.

(4) Sequence analysis In order to perform homology search of gene sequences, extraction was performed using a DNA extractor (model Biomek, manufactured by Beckman Coulter) and purified using a multiscreen 96-well filter plate (manufactured by Millipore). The resulting plasmid DNA was used for sequence analysis. Sequence analysis is performed using a DNA sequencer (ABI P
RISM 3700. PE Applied Biosystems, CA, USA) and determined by the dye terminator cycle sequence method. Based on the GenBank / EMBL database, sequence homology search was performed using the BLAST program.

(5) Amplification of cDNA As a vector for preparing a cDNA library, λZAP and λF
LC-1 was used. Inserted in vector for library
The cDNA was amplified by PCR using primers complementary to the sequences of the vector on both sides of the cDNA. The sequences of the primers are as follows.

FL forward 1224: 5'-CGCCAGGGTTTTCCCAGT
CACGA (SEQ ID NO: 73) FL reverse 1233: 5'-AGCGGATAACAATTTCACACAGGA (SEQ ID NO: 74) 100 μl PCR mixture (0.25 mM dNTP, 0.2 μM PCR primer, 1 X Ex Taq buffer, 1.25 U Ex Taq polymerase (Takara Shuzo) ), A plasmid (1-2 ng) was added as a template. PCR was performed by first reacting at 94 ° C for 3 minutes, followed by 35 cycles of 95 ° C for 1 minute, 60 ° C for 30 seconds and 72 ° C for 3 minutes, and finally at 72 ° C for 3 minutes. went. After ethanol precipitation of the PCR product, 25 μl of 3X
Dissolved in SSC. The quality of the obtained DNA and the amplification efficiency of PCR were confirmed by electrophoresis using 0.7% agarose gel.

(6) Preparation of cDNA microarray gene tip microarray stamp machine GTMASS SYSTEM
Using (Nippon Laser & Electronics Lab.), 0.5
μl PCR product (500-1000 ng / ml) was loaded from a 384-well microtiter plate, and 48 microslide glasses (model Super Aldehyde substrate, Telechem)
0.5 nl each was spotted on the surface of International) at intervals of 300 μm. The slide after spotting was dried in an atmosphere having a relative humidity of 30% or less and subjected to ultraviolet irradiation for a crosslinking reaction.

Thereafter, the slide was shaken in 0.2% SDS for 2 minutes three times, and then the slide was soaked in distilled water.
I went there. Then place the slides on the slide rack,
The slide rack was placed in the chamber containing hot water and left for 2 minutes. Then, a blocking solution (containing 1 g of borohydride, 300 ml of PBS and 90 ml of 100% ethanol) was poured into the chamber. After gently shaking the glass chamber containing the slide rack, the slide rack was transferred to a chamber containing 0.2% SDS and gently shaken for 1 minute three times. Then, the slide rack was transferred to a glass chamber containing distilled water, shaken gently for 1 minute, and then centrifuged for 20 minutes to dry.

(7) Isolation of plant material and RNA As plant material, seeds were sown on an agar medium and cultivated for 3 weeks (Y
amaguchi-Shinozaki and Shinozaki, 1994) DREB1 in the 35S promoter of wild type and cauliflower mosaic virus
A plant of Arabidopsis thaliana (Colombian species) introduced by connecting A cDNA (Kasuga et al., 1999) was used. Drying and low temperature stress treatment are Yamaguchi-Shinozaki and Shinozaki
(1994). That is, the plants extracted from the agar medium were placed on a filter paper and dried under the conditions of 22 ° C. and 60% relative humidity. 4 plants grown at 22 ° C
Low temperature treatment was carried out by shifting to ℃. The high salt stress treatment was performed by hydroponic culture in an aqueous solution containing 250 mM NaCl.

Wild type plants were subjected to stress treatment for 2 hours or 10 hours, sampled, and then frozen and stored using liquid nitrogen. In addition, wild type and DREB1A overexpressing transformants cultivated on an agar medium containing no kanamycin were used in an experiment for identifying the target gene of DREB1A. No stress treatment was applied to the DREB1A overexpressing transformant. From plants, ISOGEN (Nippon gen
e, Tokyo, Japan) to isolate total RNA and
mRNA was isolated and purified using the otex-dT30 mRNA purification kit (Takara, Tokyo, Japan).

(8) Fluorescent labeling of probe Each mRNA sample was reverse-transcribed in the presence of Cy3 dUTP or Cy5 dUTP (Amersham Pharmacia). Specifically, the reverse transcription reaction was performed using 1 ng of λ poly A ⁺ RNA-A (TX
802, Takara Shuzo Co., Ltd.) containing 1 μg of modified poly (A) ⁺ , 50 ng /
μl 12-18mer oligo dT primer (manufactured by Life Technology), 0.5 mM dATP, dGDP and dCTP, 0.2 mM, respectively
DTTP, 0.1 mM Cy3 dUTP or Cy5 dUTP, 100 U Rna
1x Superscript first-strand buffer (50 mM containing se inhibitor, 10 mM DTT and 200 U Superscript II reverse transcriptase)
Contains Tris-HCl, pH 8.3, 75 mM KCl, 3 mM MgCl ₂ , 20 mM DTT. The total volume was 20 μl in Life Technology Co., Ltd.).

The reaction solution having the above composition was incubated at 42 ° C. for 35 minutes, 200 U of Superscript II reverse transcriptase was added, and the mixture was further incubated at 42 ° C. for 35 minutes. Thereafter, 5 μl of 0.5 M EDTA, 10 μl of 1N sodium hydroxide and 20 μl of distilled water were added to the reaction solution to stop the enzymatic reaction in the reaction solution and decompose the template. Then, the reaction solution was incubated at 65 ° C. for 1 hour. After that, the reaction solution was added with 1 M Tris-HCl (pH
7.5) was neutralized.

The reaction solution was Microcon 30 micro concentrat.
or (made by Amicon). The process of adding 250 μl of TE buffer and centrifuging until the buffer amount became 10 μl, and discarding the effluent was repeated 4 times. The probe contained in the reaction solution was recovered by centrifugation, and a few μl of distilled water was added.
To the resulting probe was added 5.1 μl 20 X SSC, 2 μg / μl yeast tRNA, 4.8 μl 2% SDS. In addition, the sample
The cells were denatured at 100 ° C. for 2 minutes, left at room temperature for 5 minutes, and then used for hybridization.

(9) The microarray hybridization and the scanning probe were subjected to high speed centrifugation for 1 minute using a benchtop micro centrifuge. The probe was placed in the center of the array and covered with a coverslip to avoid foaming. Drop 4 drops of 5 μl 3 X SSC on a glass slide,
The chamber was kept at an appropriate humidity to prevent the probe from drying out during hybridization. The slide glass was placed in a cassette for hybridization (THC-1, BM instrument), sealed, and then treated at 65 ° C. for 12 to 16 hours. Take out the slide glass and place it on the slide rack.
Carefully remove the coverslip in (2X SSC, 0.03% SDS) and shake the rack to wash and wash the rack with solution 2 (1X SS
C) and washed for 2 minutes. Further rack 3
(0.05X SSC), leave for 2 minutes, and centrifuge (2500g, 1mi
n) and dried.

Scanning Laser Microscope (ScanArray4000; GSI
Lumonics, Watertown, MA) 10 per pixel
The microarray was scanned with a resolution of μm. QuantArray is a program for microarray data analysis.
Version 2.0 (GSI Lumonics) was used. Background fluorescence was calculated based on the fluorescence signals of negative control genes (nicotinic acetylcholine receptor ε subunit (nAChRE) gene and mouse glucocorticoid receptor homolog gene). Samples with a fluorescence signal value of less than 1000 (which means less than twice the background fluorescence signal value) were not analyzed. Gene clustering analysis was performed using Genespring (manufactured by Silicon Genetics).

(10) Northern analysis Northern analysis was performed using total RNA (Yamaguchi
-Shinozaki and Shinozaki, 1994). A DNA fragment isolated from the full-length cDNA library of Arabidopsis thaliana by PCR was used as a probe for Northern hybridization.

(11) Gene analysis program BLAST based on the genomic information of Arabidopsis thaliana in the determination database (GenBank / EMBL, ABRC) of genes encoding transcription factors
Was used to analyze the gene encoding the transcription factor.

2. Results (1) Identification of stress-inducible gene From mRNA isolated from a stress-free Arabidopsis thaliana plant, reverse transcription was performed in the presence of Cy5-dUTP to prepare a fluorescence-labeled cDNA. A second probe labeled with Cy3-dUTP was prepared from a plant that had been subjected to a dry treatment, a low temperature treatment (2 hours) or a high salt concentration treatment. Both probes were simultaneously hybridized to a cDNA microarray containing about 7,000 Arabidopsis thaliana cDNA clones and then a pseudocolor image was prepared.

Genes induced and repressed by stress are represented by red and green signals, respectively. Genes expressed at approximately the same level in both treatments give a yellow signal. The intensity of each spot corresponds to the absolute value of the expression level of each gene. The cold-inducible gene (rd29A) gives a red signal and the control fragment (internal control) gives a yellow signal.

As a result of scanning the microarray, 277 genes that were specifically induced by the drying treatment were identified, 53 genes that were specifically induced by the low temperature treatment were identified, and by the high salt treatment, Guided by 19
Four genes were identified. A gene induced by an expression ratio 5 times or more that of the control fragment was defined as a gene induced by various stresses.

As a result of the analysis using the database, 35 kinds of transcription factors that could be classified into the following families could be identified. Although RAFL05-21-L12 could not be classified into the following families, the one obtained by converting the nucleic acid base sequence into an amino acid sequence was used in the GenBank Database.
A blast X search was performed on the amino acid sequence Data registered in
at shock transcription factor-like protein) and E-va
Since it was homologous in the score of lue: e- ¹⁰⁰ , it could be identified as a transcription factor. That is, 36 types of transcription factors could be identified by this example. DREB family: RAFL05-11-M11, RAFL06-11-K21, RA
FL05-16-H23, RAFL08-16-D06 ERF family: RAFL08-16-G17, RAFL06-08-H20 Zinc finger family: RAFL07-10-G04, RAFL0
4-17-D16, RAFL05-19-M20, RAFL08-11-M13, RAFL04-15-
K19, RAFL05-11-L01, RAFL05-14-C11, RAFL05-19-G24,
RAFL05-20-N02 WRKY Family: RAFL05-18-H12, RAFL05-19-E19, RA
FL06-10-D22, RAFL06-12-M01 MYB family: RAFL05-14-D24, RAFL05-20-N17, RAF
L04-17-F21 bHLH Family: RAFL09-12-N16 NAC Family: RAFL05-19-I05, RAFL05-21-I22, RAF
L08-11-H20, RAFL05-21-C17, RAFL05-08-D06 Homeodomain family: RAFL05-20-M16, RAFL11-
01-J18, RAFL11-09-C20 bZIP family: RAFL05-18-N16, RAFL11-10-D10, RA
FL04-17-N22, RAFL05-09-G15

(3) Relationship between various stress treatment times and expression ratios Regarding the genes encoding the 36 kinds of various stress-responsive transcription factors isolated as described above, the various stress treatment times and expression were as follows. The results of examining the relationship with the ratio are shown in FIGS. The correspondence between the gene names shown in FIGS. 1 to 57 and the stress treatment is shown in Table 2.

[0091]

[Table 2]

1 to 57, the vertical axis represents the gene expression ratio, which is calculated as follows. In addition,
In the following formula, FI represents fluorescence intensity. Expression ratio = [(FI of each cDNA under stress-loaded conditions) / (FI of each cDNA under stress-free conditions)] / [(FI of control fragment under stress-loaded conditions) / (No stress-loaded conditions) FI of the control fragment in the above)] As shown in FIGS. 1 to 57, the genes encoding the stress-responsive transcription factors isolated by this method have different profiles, but are expressed by the addition of various stresses. You can see that it is being induced.

[0093]

INDUSTRIAL APPLICABILITY The present invention provides a gene encoding a stress-responsive transcription factor. The gene of the present invention is
It is useful because it can be used for molecular breeding of environmental stress resistant plants.

[0094]

[Sequence list]                       SEQUENCE LISTING <110> RIKEN <120> A gene cording stress-responsive transcription factor <130> RJH13-142T <140> <141> <160> 74 <170> PatentIn Ver. 2.1 <210> 1 <211> 588 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (585) <400> 1 atg gtg aag caa gcg atg aag gaa gag gag aag aag aga aac acg gcg 48 Met Val Lys Gln Ala Met Lys Glu Glu Glu Lys Lys Arg Asn Thr Ala   1 5 10 15 atg cag tca aag tac aaa gga gtg agg aag agg aaa tgg gga aaa tgg 96 Met Gln Ser Lys Tyr Lys Gly Val Arg Lys Arg Lys Trp Gly Lys Trp              20 25 30 gta tcg gag atc aga ctt cca cac agc aga gaa cga att tgg tta ggc 144 Val Ser Glu Ile Arg Leu Pro His Ser Arg Glu Arg Ile Trp Leu Gly          35 40 45 tct tac gac act ccc gag aag gcg gcg cgt gct ttc gac gcc gct caa 192 Ser Tyr Asp Thr Pro Glu Lys Ala Ala Arg Ala Phe Asp Ala Ala Gln      50 55 60 ttt tgt ctc cgc ggc ggc gat gct aat ttc aat ttc cct aat aat cca 240 Phe Cys Leu Arg Gly Gly Asp Ala Asn Phe Asn Phe Pro Asn Asn Pro  65 70 75 80 ccg tcg atc tcc gta gaa aag tcg ttg acg cct ccg gag att cag gaa 288 Pro Ser Ile Ser Val Glu Lys Ser Leu Thr Pro Pro Glu Ile Gln Glu                  85 90 95 gct gct gct aga ttc gct aac aca ttc caa gac att gtc aag gga gaa 336 Ala Ala Ala Arg Phe Ala Asn Thr Phe Gln Asp Ile Val Lys Gly Glu             100 105 110 gaa gaa tcg ggt tta gta ccc gga tcc gag atc cga cca gag tct cct 384 Glu Glu Ser Gly Leu Val Pro Gly Ser Glu Ile Arg Pro Glu Ser Pro         115 120 125 tct aca tct gca tct gtt gct aca tcg acg gtg gat tat gat ttt tcg 432 Ser Thr Ser Ala Ser Val Ala Thr Ser Thr Val Asp Tyr Asp Phe Ser     130 135 140 ttt ttg gat ttg ctt ccg atg aat ttc ggg ttt gat tcc ttc tcc gac 480 Phe Leu Asp Leu Leu Pro Met Asn Phe Gly Phe Asp Ser Phe Ser Asp 145 150 155 160 gac ttc tct ggc ttc tcc ggt ggt gat cga ttt aca gag att tta ccc 528 Asp Phe Ser Gly Phe Ser Gly Gly Asp Arg Phe Thr Glu Ile Leu Pro                 165 170 175 atc gaa gat tac gga gga gag agt tta tta gat gaa tct ttg att ctt 576 Ile Glu Asp Tyr Gly Gly Glu Ser Leu Leu Asp Glu Ser Leu Ile Leu             180 185 190 tgg gat ttt tga 588 Trp Asp Phe         195 <210> 2 <211> 195 <212> PRT <213> Arabidopsis thaliana <400> 2 Met Val Lys Gln Ala Met Lys Glu Glu Glu Lys Lys Arg Asn Thr Ala   1 5 10 15 Met Gln Ser Lys Tyr Lys Gly Val Arg Lys Arg Lys Trp Gly Lys Trp              20 25 30 Val Ser Glu Ile Arg Leu Pro His Ser Arg Glu Arg Ile Trp Leu Gly          35 40 45 Ser Tyr Asp Thr Pro Glu Lys Ala Ala Arg Ala Phe Asp Ala Ala Gln      50 55 60 Phe Cys Leu Arg Gly Gly Asp Ala Asn Phe Asn Phe Pro Asn Asn Pro  65 70 75 80 Pro Ser Ile Ser Val Glu Lys Ser Leu Thr Pro Pro Glu Ile Gln Glu                  85 90 95 Ala Ala Ala Arg Phe Ala Asn Thr Phe Gln Asp Ile Val Lys Gly Glu             100 105 110 Glu Glu Ser Gly Leu Val Pro Gly Ser Glu Ile Arg Pro Glu Ser Pro         115 120 125 Ser Thr Ser Ala Ser Val Ala Thr Ser Thr Val Asp Tyr Asp Phe Ser     130 135 140 Phe Leu Asp Leu Leu Pro Met Asn Phe Gly Phe Asp Ser Phe Ser Asp 145 150 155 160 Asp Phe Ser Gly Phe Ser Gly Gly Asp Arg Phe Thr Glu Ile Leu Pro                 165 170 175 Ile Glu Asp Tyr Gly Gly Glu Ser Leu Leu Asp Glu Ser Leu Ile Leu             180 185 190 Trp Asp Phe         195 <210> 3 <211> 1290 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (81) .. (1088) <400> 3 aacaaatctc tctgtttctc ccgctcttgc tctgttttct caaagacaaa agaggacatc 60 gtcgttgact cctcttctct atg gct act gct aag aac aag gga aaa tca atc 113                       Met Ala Thr Ala Lys Asn Lys Gly Lys Ser Ile                         1 5 10 agg gtc ctt ggt acc agt gaa gca gag aaa aag gat gag atg gag ttg 161 Arg Val Leu Gly Thr Ser Glu Ala Glu Lys Lys Asp Glu Met Glu Leu              15 20 25 gag gag gag ttc cag ttt agt agc ggc aag tat aaa gat tcg ggt cct 209 Glu Glu Glu Phe Gln Phe Ser Ser Gly Lys Tyr Lys Asp Ser Gly Pro          30 35 40 ggc tcg gac atg tgg tta gga gat gct tcc tct acg tct cca aga agt 257 Gly Ser Asp Met Trp Leu Gly Asp Ala Ser Ser Thr Ser Pro Arg Ser      45 50 55 ctt agg aag act aga acc ttt gac cga cat aat ccc tat ctc gta tct 305 Leu Arg Lys Thr Arg Thr Phe Asp Arg His Asn Pro Tyr Leu Val Ser  60 65 70 75 tct tat gct act cct cag ccg cca aca aca act aca tgc tct gtc tct 353 Ser Tyr Ala Thr Pro Gln Pro Pro Thr Thr Thr Thr Cys Ser Val Ser                  80 85 90 ttt ccc ttt tac ctc cct cca gcg att caa aat caa caa cga ttt tta 401 Phe Pro Phe Tyr Leu Pro Pro Ala Ile Gln Asn Gln Gln Arg Phe Leu              95 100 105 cac ccg aat gac cct tca gga caa aga cag caa caa atg atc tcg ttt 449 His Pro Asn Asp Pro Ser Gly Gln Arg Gln Gln Gln Met Ile Ser Phe         110 115 120 gat cct caa caa cag gtg caa cca tat gtt gca caa cag cag caa caa 497 Asp Pro Gln Gln Gln Val Gln Pro Tyr Val Ala Gln Gln Gln Gln Gln     125 130 135 caa caa cat cta ttg cag tac tgg aga gac att ctg aag ctg agt ccg 545 Gln Gln His Leu Leu Gln Tyr Trp Arg Asp Ile Leu Lys Leu Ser Pro 140 145 150 155 agc gga aga atg atg atg atg aac atg tta aga caa gaa agc gat ctg 593 Ser Gly Arg Met Met Met Met Asn Met Leu Arg Gln Glu Ser Asp Leu                 160 165 170 cca ctg acg agg cca ccg gtt caa ccc ttc agc gcc acc aag cta tat 641 Pro Leu Thr Arg Pro Pro Val Gln Pro Phe Ser Ala Thr Lys Leu Tyr             175 180 185 aga ggt gtc agg caa cgc cac tgg gga aaa tgg gtt gcc gag atc cgt 689 Arg Gly Val Arg Gln Arg His Trp Gly Lys Trp Val Ala Glu Ile Arg         190 195 200 aag cca cga aac agg aca cgt ctc tgg cta ggg aca ttc gat aca gca 737 Lys Pro Arg Asn Arg Thr Arg Leu Trp Leu Gly Thr Phe Asp Thr Ala     205 210 215 gaa gaa gcc gcc atg gcc tac gac cgc gag gcc ttc aag ttg agg gga 785 Glu Glu Ala Ala Met Ala Tyr Asp Arg Glu Ala Phe Lys Leu Arg Gly 220 225 230 235 gag acc gct agg ctc aat ttc cct gaa ctt ttt ctc aat aaa caa gag 833 Glu Thr Ala Arg Leu Asn Phe Pro Glu Leu Phe Leu Asn Lys Gln Glu                 240 245 250 cca act ccc gtg cat cag aaa caa tgt gag acg ggg act act agt gaa 881 Pro Thr Pro Val His Gln Lys Gln Cys Glu Thr Gly Thr Thr Ser Glu             255 260 265 gac tca agc aga aga gga gag gat gat tcg agc acg gca ttg gca gta 929 Asp Ser Ser Arg Arg Gly Glu Asp Asp Ser Ser Thr Ala Leu Ala Val         270 275 280 gga ggg gtg agt gag gag acg ggt tgg gct gag gca tgg ttc aat gca 977 Gly Gly Val Ser Glu Glu Thr Gly Trp Ala Glu Ala Trp Phe Asn Ala     285 290 295 att cca gag gaa tgg gga cct gga agc cct cta tgg gat gat tac cac 1025 Ile Pro Glu Glu Trp Gly Pro Gly Ser Pro Leu Trp Asp Asp Tyr His 300 305 310 315 ttt ccc att tct aac cat aag gac gat ctt gac gcc aca caa aac tct 1073 Phe Pro Ile Ser Asn His Lys Asp Asp Leu Asp Ala Thr Gln Asn Ser                 320 325 330 tct tct gat aca att taggaccttt gttagaatat agatatgctt agttgtatga 1128 Ser Ser Asp Thr Ile             335 ctgatctagc ttgtgttttt tttttgggtg gagacagttt ttgtcatctt ccacatttta 1188 gattctattt tcgaccatca tttttttctt gatcggtgac tatgaatcta atggggtcaa 1248 tcattttcac atataaaact taagtatttg gtgtttgtac tt 1290 <210> 4 <211> 336 <212> PRT <213> Arabidopsis thaliana <400> 4 Met Ala Thr Ala Lys Asn Lys Gly Lys Ser Ile Arg Val Leu Gly Thr   1 5 10 15 Ser Glu Ala Glu Lys Lys Asp Glu Met Glu Leu Glu Glu Glu Phe Gln              20 25 30 Phe Ser Ser Gly Lys Tyr Lys Asp Ser Gly Pro Gly Ser Asp Met Trp          35 40 45 Leu Gly Asp Ala Ser Ser Thr Ser Pro Arg Ser Leu Arg Lys Thr Arg      50 55 60 Thr Phe Asp Arg His Asn Pro Tyr Leu Val Ser Ser Tyr Ala Thr Pro  65 70 75 80 Gln Pro Pro Thr Thr Thr Thr Cys Ser Val Ser Phe Pro Phe Tyr Leu                  85 90 95 Pro Pro Ala Ile Gln Asn Gln Gln Arg Phe Leu His Pro Asn Asp Pro             100 105 110 Ser Gly Gln Arg Gln Gln Gln Met Ile Ser Phe Asp Pro Gln Gln Gln         115 120 125 Val Gln Pro Tyr Val Ala Gln Gln Gln Gln Gln Gln Gln His Leu Leu     130 135 140 Gln Tyr Trp Arg Asp Ile Leu Lys Leu Ser Pro Ser Gly Arg Met Met 145 150 155 160 Met Met Asn Met Leu Arg Gln Glu Ser Asp Leu Pro Leu Thr Arg Pro                 165 170 175 Pro Val Gln Pro Phe Ser Ala Thr Lys Leu Tyr Arg Gly Val Arg Gln             180 185 190 Arg His Trp Gly Lys Trp Val Ala Glu Ile Arg Lys Pro Arg Asn Arg         195 200 205 Thr Arg Leu Trp Leu Gly Thr Phe Asp Thr Ala Glu Glu Ala Ala Met     210 215 220 Ala Tyr Asp Arg Glu Ala Phe Lys Leu Arg Gly Glu Thr Ala Arg Leu 225 230 235 240 Asn Phe Pro Glu Leu Phe Leu Asn Lys Gln Glu Pro Thr Pro Val His                 245 250 255 Gln Lys Gln Cys Glu Thr Gly Thr Thr Ser Glu Asp Ser Ser Arg Arg             260 265 270 Gly Glu Asp Asp Ser Ser Thr Ala Leu Ala Val Gly Gly Val Ser Glu         275 280 285 Glu Thr Gly Trp Ala Glu Ala Trp Phe Asn Ala Ile Pro Glu Glu Trp     290 295 300 Gly Pro Gly Ser Pro Leu Trp Asp Asp Tyr His Phe Pro Ile Ser Asn 305 310 315 320 His Lys Asp Asp Leu Asp Ala Thr Gln Asn Ser Ser Ser Asp Thr Ile                 325 330 335 <210> 5 <211> 879 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (876) <400> 5 atg gac ttt gac gag gag cta aat ctt tgt att acg aaa ggt aaa aat 48 Met Asp Phe Asp Glu Glu Leu Asn Leu Cys Ile Thr Lys Gly Lys Asn   1 5 10 15 gtt gat cat tct ttt gga gga gaa gct tct tcc acg tcc cca aga tct 96 Val Asp His Ser Phe Gly Gly Glu Ala Ser Ser Thr Ser Pro Arg Ser              20 25 30 atg aag aaa atg aag agt cct agt cgt cct aaa ccc tat ttc caa tcc 144 Met Lys Lys Met Lys Ser Pro Ser Arg Pro Lys Pro Tyr Phe Gln Ser          35 40 45 tct tct tct cct tat tcg tta gag gct ttc cct ttt tct ctc gat cca 192 Ser Ser Ser Pro Tyr Ser Leu Glu Ala Phe Pro Phe Ser Leu Asp Pro      50 55 60 aca ctt cag aat cag caa caa caa ctc gga tca tac gtt ccg gta ctt 240 Thr Leu Gln Asn Gln Gln Gln Gln Leu Gly Ser Tyr Val Pro Val Leu  65 70 75 80 gag caa cga caa gac ccg aca atg caa ggc cag aag caa atg atc tcc 288 Glu Gln Arg Gln Asp Pro Thr Met Gln Gly Gln Lys Gln Met Ile Ser                  85 90 95 ttt agt cct caa caa caa caa cag cag cag cag tat atg gcc cag tac 336 Phe Ser Pro Gln Gln Gln Gln Gln Gln Gln Gln Tyr Met Ala Gln Tyr             100 105 110 tgg agt gac aca ttg aat ctg agt cca aga gga aga atg atg atg atg 384 Trp Ser Asp Thr Leu Asn Leu Ser Pro Arg Gly Arg Met Met Met Met         115 120 125 atg agc caa gaa gct gtt caa cct tac atc gca acg aag ctg tac aga 432 Met Ser Gln Glu Ala Val Gln Pro Tyr Ile Ala Thr Lys Leu Tyr Arg     130 135 140 gga gtg aga caa cgt caa tgg gga aaa tgg gtc gca gag atc cgt aag 480 Gly Val Arg Gln Arg Gln Trp Gly Lys Trp Val Ala Glu Ile Arg Lys 145 150 155 160 cca cga agc agg gca cgt ctt tgg ctt ggt acc ttt gat aca gct gaa 528 Pro Arg Ser Arg Ala Arg Leu Trp Leu Gly Thr Phe Asp Thr Ala Glu                 165 170 175 gaa gct gcc atg gcc tac gac cgc caa gcc ttc aaa tta cga ggc cac 576 Glu Ala Ala Met Ala Tyr Asp Arg Gln Ala Phe Lys Leu Arg Gly His             180 185 190 agc gca aca ctg aat ttc ccg gag cat ttt gtg aat aag gaa agc gag 624 Ser Ala Thr Leu Asn Phe Pro Glu His Phe Val Asn Lys Glu Ser Glu         195 200 205 ctg cat gat tca aac tcg tcg gat cag aaa gaa cct gaa acg cca cag 672 Leu His Asp Ser Asn Ser Ser Asp Gln Lys Glu Pro Glu Thr Pro Gln     210 215 220 cca agc gag gtt aac ttg gag agc aag gaa cta ccg gtg att gat gtt 720 Pro Ser Glu Val Asn Leu Glu Ser Lys Glu Leu Pro Val Ile Asp Val 225 230 235 240 ggg aga gag gaa ggt atg gct gag gca tgg tac aat gcc att aca tcg 768 Gly Arg Glu Glu Gly Met Ala Glu Ala Trp Tyr Asn Ala Ile Thr Ser                 245 250 255 gga tgg ggt cct gaa agt cct ctt tgg gat gat ttg gat agt tct cat 816 Gly Trp Gly Pro Glu Ser Pro Leu Trp Asp Asp Leu Asp Ser Ser His             260 265 270 cag ttt tca tca gaa agc tca tct tct tct cct ctc tct tgt cct atg 864 Gln Phe Ser Ser Glu Ser Ser Ser Ser Ser Pro Leu Ser Cys Pro Met         275 280 285 agg cct ttc ttt tga 879 Arg Pro Phe Phe     290 <210> 6 <211> 292 <212> PRT <213> Arabidopsis thaliana <400> 6 Met Asp Phe Asp Glu Glu Leu Asn Leu Cys Ile Thr Lys Gly Lys Asn   1 5 10 15 Val Asp His Ser Phe Gly Gly Glu Ala Ser Ser Thr Ser Pro Arg Ser              20 25 30 Met Lys Lys Met Lys Ser Pro Ser Arg Pro Lys Pro Tyr Phe Gln Ser          35 40 45 Ser Ser Ser Pro Tyr Ser Leu Glu Ala Phe Pro Phe Ser Leu Asp Pro      50 55 60 Thr Leu Gln Asn Gln Gln Gln Gln Leu Gly Ser Tyr Val Pro Val Leu  65 70 75 80 Glu Gln Arg Gln Asp Pro Thr Met Gln Gly Gln Lys Gln Met Ile Ser                  85 90 95 Phe Ser Pro Gln Gln Gln Gln Gln Gln Gln Gln Tyr Met Ala Gln Tyr             100 105 110 Trp Ser Asp Thr Leu Asn Leu Ser Pro Arg Gly Arg Met Met Met Met         115 120 125 Met Ser Gln Glu Ala Val Gln Pro Tyr Ile Ala Thr Lys Leu Tyr Arg     130 135 140 Gly Val Arg Gln Arg Gln Trp Gly Lys Trp Val Ala Glu Ile Arg Lys 145 150 155 160 Pro Arg Ser Arg Ala Arg Leu Trp Leu Gly Thr Phe Asp Thr Ala Glu                 165 170 175 Glu Ala Ala Met Ala Tyr Asp Arg Gln Ala Phe Lys Leu Arg Gly His             180 185 190 Ser Ala Thr Leu Asn Phe Pro Glu His Phe Val Asn Lys Glu Ser Glu         195 200 205 Leu His Asp Ser Asn Ser Ser Asp Gln Lys Glu Pro Glu Thr Pro Gln     210 215 220 Pro Ser Glu Val Asn Leu Glu Ser Lys Glu Leu Pro Val Ile Asp Val 225 230 235 240 Gly Arg Glu Glu Gly Met Ala Glu Ala Trp Tyr Asn Ala Ile Thr Ser                 245 250 255 Gly Trp Gly Pro Glu Ser Pro Leu Trp Asp Asp Leu Asp Ser Ser His             260 265 270 Gln Phe Ser Ser Glu Ser Ser Ser Ser Ser Pro Leu Ser Cys Pro Met         275 280 285 Arg Pro Phe Phe     290 <210> 7 <211> 1499 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (644) .. (1222) <400> 7 tggtatcggt gaggctgaga gttattcact tacaaaaaaa aaaaaaaact tgagtgtaac 60 caaaaaaaaa aagttgatat actttctggt tttctcctta acttttattc tttacaaatc 120 catccccctt agatctgttt atttcccgct actttgattc atttctgtta gtaatctgtc 180 tttcgtatag aagaaaactg atttcttggt ttgtattttc ttaaagagat caatcttttt 240 ttatttttga tcttcttgtg tttttttttc tttgtagaat taatcgtttg tgagggtatt 300 tttttaattc cctcctctca gaaatctaca cagaggtttt ttattttata aacctctttt 360 ttcgattttc ttgaaaacaa aaaatcctgt tctttacttt ttttacaaga acaagggaaa 420 aaaatttctt tttattagaa atgacaactt ctatggattt ttacagtaac aaaacgtttc 480 aacaatctga tccattcggt ggtgaattaa tggaagcgct ttacctttta tcaaaagccc 540 ttccaacgat tcatccgcgt ttgcgttctc tctacccgct ccaatttcat acgggtcgga 600 tctccactca ttttctcacc atcttagtcc taaaccggtc tca atg aaa caa acc 655                                                 Met Lys Gln Thr                                                   1 ggt act tcc gcg gct aaa ccg acg aag cta tac aga gga gtg aga caa 703 Gly Thr Ser Ala Ala Lys Pro Thr Lys Leu Tyr Arg Gly Val Arg Gln   5 10 15 20 cgt cac tgg gga aaa tgg gtg gct gag att cgt tta ccg agg aat cga 751 Arg His Trp Gly Lys Trp Val Ala Glu Ile Arg Leu Pro Arg Asn Arg                  25 30 35 act cga ctt tgg ctc gga aca ttc gac acg gcg gag gaa gct gct tta 799 Thr Arg Leu Trp Leu Gly Thr Phe Asp Thr Ala Glu Glu Ala Ala Leu              40 45 50 gct tat gac aag gcg gcg tat aag ctc cga gga gat ttt gcg cgg ctt 847 Ala Tyr Asp Lys Ala Ala Tyr Lys Leu Arg Gly Asp Phe Ala Arg Leu          55 60 65 aat ttc cct gat ctc cgt cat aac gac gag tat caa cct ctt caa tca 895 Asn Phe Pro Asp Leu Arg His Asn Asp Glu Tyr Gln Pro Leu Gln Ser      70 75 80 tca gtc gac gct aag ctt gaa gct att tgt caa aac tta gct gag acg 943 Ser Val Asp Ala Lys Leu Glu Ala Ile Cys Gln Asn Leu Ala Glu Thr  85 90 95 100 acg cag aaa cag gtg aga tca acg aag aag tct tct tct cgg aaa cgt 991 Thr Gln Lys Gln Val Arg Ser Thr Lys Lys Ser Ser Ser Arg Lys Arg                 105 110 115 tca tca acc gtc gca gtg aaa cta ccg gag gag gac tac tct agc gcc 1039 Ser Ser Thr Val Ala Val Lys Leu Pro Glu Glu Asp Tyr Ser Ser Ala             120 125 130 gga tct tcg ccg ctg tta acg gag agt tat gga tct ggt gga tct tct 1087 Gly Ser Ser Pro Leu Leu Thr Glu Ser Tyr Gly Ser Gly Gly Ser Ser         135 140 145 tcg ccg ttg tcg gag ctg acg ttt ggt gat acg gag gag gag att cag 1135 Ser Pro Leu Ser Glu Leu Thr Phe Gly Asp Thr Glu Glu Glu Ile Gln     150 155 160 ccg ccg tgg aac gag aac gcg ttg gag aag tat ccg tcg tac gag atc 1183 Pro Pro Trp Asn Glu Asn Ala Leu Glu Lys Tyr Pro Ser Tyr Glu Ile 165 170 175 180 gat tgg gat tcg att ctt cag tgt tcg agt ctt gta aat tagatgttgc 1232 Asp Trp Asp Ser Ile Leu Gln Cys Ser Ser Leu Val Asn                 185 190 cataggggta ttttagggac tttagagctc tctgcgatgg agtttttggt cattgcagag 1292 attttattat tattaagggg gtttgttatg ttaatatcaa ataagtttat ctactttgat 1352 gttaattagt gttaatctct gcgtcggtcc aagctgtttt tttttggcat gcttcgaccg 1412 tgtgagattt cttatgtaat ttttgtagtt ccttgatttt cttagttcaa gttaaattgg 1472 cacaaaagag caaaaaaaaa aaaaaaa 1499 <210> 8 <211> 193 <212> PRT <213> Arabidopsis thaliana <400> 8 Met Lys Gln Thr Gly Thr Ser Ala Ala Lys Pro Thr Lys Leu Tyr Arg   1 5 10 15 Gly Val Arg Gln Arg His Trp Gly Lys Trp Val Ala Glu Ile Arg Leu              20 25 30 Pro Arg Asn Arg Thr Arg Leu Trp Leu Gly Thr Phe Asp Thr Ala Glu          35 40 45 Glu Ala Ala Leu Ala Tyr Asp Lys Ala Ala Tyr Lys Leu Arg Gly Asp      50 55 60 Phe Ala Arg Leu Asn Phe Pro Asp Leu Arg His Asn Asp Glu Tyr Gln  65 70 75 80 Pro Leu Gln Ser Ser Val Asp Ala Lys Leu Glu Ala Ile Cys Gln Asn                  85 90 95 Leu Ala Glu Thr Thr Gln Lys Gln Val Arg Ser Thr Lys Lys Ser Ser             100 105 110 Ser Arg Lys Arg Ser Ser Thr Val Ala Val Lys Leu Pro Glu Glu Asp         115 120 125 Tyr Ser Ser Ala Gly Ser Ser Pro Leu Leu Thr Glu Ser Tyr Gly Ser     130 135 140 Gly Gly Ser Ser Ser Pro Leu Ser Glu Leu Thr Phe Gly Asp Thr Glu 145 150 155 160 Glu Glu Ile Gln Pro Pro Trp Asn Glu Asn Ala Leu Glu Lys Tyr Pro                 165 170 175 Ser Tyr Glu Ile Asp Trp Asp Ser Ile Leu Gln Cys Ser Ser Leu Val             180 185 190 Asn <210> 9 <211> 962 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (77) .. (880) <400> 9 acaccggaca ttttgaaatc tcaacaagaa ccaaaccaaa caacaaaaaa acattcttaa 60 taattatctt tctgtt atg tcg atg acg gcg gat tct caa tct gat tat gct 112                   Met Ser Met Thr Ala Asp Ser Gln Ser Asp Tyr Ala                     1 5 10 ttt ctt gag tcc ata cga cga cac tta cta gga gaa tcg gag ccg ata 160 Phe Leu Glu Ser Ile Arg Arg His Leu Leu Gly Glu Ser Glu Pro Ile          15 20 25 ctc agt gag tcg aca gcg agt tcg gtt act caa tct tgt gta acc ggt 208 Leu Ser Glu Ser Thr Ala Ser Ser Val Thr Gln Ser Cys Val Thr Gly      30 35 40 cag agc att aaa ccg gtg tac gga cga aac cct agc ttt agc aaa ctg 256 Gln Ser Ile Lys Pro Val Tyr Gly Arg Asn Pro Ser Phe Ser Lys Leu  45 50 55 60 tat cct tgc ttc acc gag agc tgg gga gat ttg ccg ttg aaa gaa aac 304 Tyr Pro Cys Phe Thr Glu Ser Trp Gly Asp Leu Pro Leu Lys Glu Asn                  65 70 75 gat tct gag gat atg tta gtt tac ggt atc ctc aac gac gcc ttt cac 352 Asp Ser Glu Asp Met Leu Val Tyr Gly Ile Leu Asn Asp Ala Phe His              80 85 90 ggc ggt tgg gag ccg tct tct tcg tct tcc gac gaa gat cgt agc tct 400 Gly Gly Trp Glu Pro Ser Ser Ser Ser Ser Ser Asp Glu Asp Arg Ser Ser          95 100 105 ttc ccg agt gtt aag atc gag act ccg gag agt ttc gcg gcg gtg gat 448 Phe Pro Ser Val Lys Ile Glu Thr Pro Glu Ser Phe Ala Ala Val Asp     110 115 120 tct gtt ccg gtc aag aag gag aag acg agt cct gtt tcg gcg gcg gtg 496 Ser Val Pro Val Lys Lys Glu Lys Thr Ser Pro Val Ser Ala Ala Val 125 130 135 140 acg gcg gcg aag gga aag cat tat aga gga gtg aga caa agg ccg tgg 544 Thr Ala Ala Lys Gly Lys His Tyr Arg Gly Val Arg Gln Arg Pro Trp                 145 150 155 ggg aaa ttt gcg gcg gag att aga gac ccg gcg aag aac gga gct agg 592 Gly Lys Phe Ala Ala Glu Ile Arg Asp Pro Ala Lys Asn Gly Ala Arg             160 165 170 gtt tgg tta gga acg ttt gag acg gcg gag gac gcg gcg ttg gct tac 640 Val Trp Leu Gly Thr Phe Glu Thr Ala Glu Asp Ala Ala Leu Ala Tyr         175 180 185 gac aga gct gct ttc agg atg cgt ggt tcc cgc gct ttg ttg aat ttt 688 Asp Arg Ala Ala Phe Arg Met Arg Gly Ser Arg Ala Leu Leu Asn Phe     190 195 200 ccg ttg aga gtt aat tca gga gaa ccc gac ccg gtt cga atc aag tcc 736 Pro Leu Arg Val Asn Ser Gly Glu Pro Asp Pro Val Arg Ile Lys Ser 205 210 215 220 aag aga tct tct ttt tct tct tct aac gag aac gga gct ccg aag aag 784 Lys Arg Ser Ser Phe Ser Ser Ser Asn Glu Asn Gly Ala Pro Lys Lys                 225 230 235 agg aga acg gtg gcc gcc ggt ggt gga atg gat aag gga ttg acg gtg 832 Arg Arg Thr Val Ala Ala Gly Gly Gly Met Asp Lys Gly Leu Thr Val             240 245 250 aag tgc gag gtt gtt gaa gtg gca cgt ggc gat cgt tta ttg gtt tta 880 Lys Cys Glu Val Val Glu Val Ala Arg Gly Asp Arg Leu Leu Val Leu         255 260 265 taattttgat ttttctttgt tggatgatta tatgattctt caaaaaagaa gaacgttaat 940 aaaaaaattc gtttattatt gt 962 <210> 10 <211> 268 <212> PRT <213> Arabidopsis thaliana <400> 10 Met Ser Met Thr Ala Asp Ser Gln Ser Asp Tyr Ala Phe Leu Glu Ser   1 5 10 15 Ile Arg Arg His Leu Leu Gly Glu Ser Glu Pro Ile Leu Ser Glu Ser              20 25 30 Thr Ala Ser Ser Val Thr Gln Ser Cys Val Thr Gly Gln Ser Ile Lys          35 40 45 Pro Val Tyr Gly Arg Asn Pro Ser Phe Ser Lys Leu Tyr Pro Cys Phe      50 55 60 Thr Glu Ser Trp Gly Asp Leu Pro Leu Lys Glu Asn Asp Ser Glu Asp  65 70 75 80 Met Leu Val Tyr Gly Ile Leu Asn Asp Ala Phe His Gly Gly Trp Glu                  85 90 95 Pro Ser Ser Ser Ser Ser Asp Glu Asp Arg Ser Ser Phe Pro Ser Val             100 105 110 Lys Ile Glu Thr Pro Glu Ser Phe Ala Ala Val Asp Ser Val Pro Val         115 120 125 Lys Lys Glu Lys Thr Ser Pro Val Ser Ala Ala Val Thr Ala Ala Lys     130 135 140 Gly Lys His Tyr Arg Gly Val Arg Gln Arg Pro Trp Gly Lys Phe Ala 145 150 155 160 Ala Glu Ile Arg Asp Pro Ala Lys Asn Gly Ala Arg Val Trp Leu Gly                 165 170 175 Thr Phe Glu Thr Ala Glu Asp Ala Ala Leu Ala Tyr Asp Arg Ala Ala             180 185 190 Phe Arg Met Arg Gly Ser Arg Ala Leu Leu Asn Phe Pro Leu Arg Val         195 200 205 Asn Ser Gly Glu Pro Asp Pro Val Arg Ile Lys Ser Lys Arg Ser Ser     210 215 220 Phe Ser Ser Ser Asn Glu Asn Gly Ala Pro Lys Lys Arg Arg Thr Val 225 230 235 240 Ala Ala Gly Gly Gly Met Asp Lys Gly Leu Thr Val Lys Cys Glu Val                 245 250 255 Val Glu Val Ala Arg Gly Asp Arg Leu Leu Val Leu             260 265 <210> 11 <211> 834 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (171) .. (746) <400> 11 aacacaattt gattacactg agcaacacaa aactggcgaa ccaacgtgac tctaacgaag 60 aaaccggcaa tggccagtat cactacaatg ccgaagaaat aacaagaatc ataaacgagc 120 cagaatatta tcccccgggt tacaacttgt ctaccaccgc aatttcaaac atg gtg 176                                                        Met Val                                                          1 tct atg ctg act aat gtt gtc tct ggt gag acc gaa ccc tcg gca tct 224 Ser Met Leu Thr Asn Val Val Ser Gly Glu Thr Glu Pro Ser Ala Ser           5 10 15 gcg aca tgg acg atg ggt cat aag aga gaa aga gaa gag ttt tct ttg 272 Ala Thr Trp Thr Met Gly His Lys Arg Glu Arg Glu Glu Phe Ser Leu      20 25 30 cct cct caa cca ttg att acc ggt tca gct gtg act aaa gaa tgt gaa 320 Pro Pro Gln Pro Leu Ile Thr Gly Ser Ala Val Thr Lys Glu Cys Glu  35 40 45 50 agc tca atg tcc ttg gag agg cca aaa aaa tat aga gga gta agg caa 368 Ser Ser Met Ser Leu Glu Arg Pro Lys Lys Tyr Arg Gly Val Arg Gln                  55 60 65 cga cca tgg gga aaa tgg gcg gcg gag att cga gac cca cac aag gcg 416 Arg Pro Trp Gly Lys Trp Ala Ala Glu Ile Arg Asp Pro His Lys Ala              70 75 80 aca cgt gta tgg ctt ggg aca ttc gag aca gcc gag gcc gcc gca aga 464 Thr Arg Val Trp Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala Ala Arg          85 90 95 gcc tat gat gcg gca gca ctt cgc ttt aga gga agc aaa gca aag ctt 512 Ala Tyr Asp Ala Ala Ala Leu Arg Phe Arg Gly Ser Lys Ala Lys Leu     100 105 110 aat ttc ccc gaa aat gtt gga act cag acg att caa cga aat tct cat 560 Asn Phe Pro Glu Asn Val Gly Thr Gln Thr Ile Gln Arg Asn Ser His 115 120 125 130 ttc ttg caa aac tct atg caa cct tct ctg aca tac atc gat caa tgt 608 Phe Leu Gln Asn Ser Met Gln Pro Ser Leu Thr Tyr Ile Asp Gln Cys                 135 140 145 cca act cta tta tct tac tct cga tgt atg gag caa caa caa cca tta 656 Pro Thr Leu Leu Ser Tyr Ser Arg Cys Met Glu Gln Gln Gln Pro Leu             150 155 160 gta ggc atg ttg cag cca aca gaa gag gaa aat cac ttt ttc gaa aaa 704 Val Gly Met Leu Gln Pro Thr Glu Glu Glu Asn His Phe Phe Glu Lys         165 170 175 cca tgg acc gaa tat gat caa tac aat tac tcc tct ttt ggt 746 Pro Trp Thr Glu Tyr Asp Gln Tyr Asn Tyr Ser Ser Phe Gly     180 185 190 taactaacat attgtcaacg ctttgtattt ctacttattc gatctaccaa ttttttctct 806 cccaatacaa cttcagtctg attattgc 834 <210> 12 <211> 192 <212> PRT <213> Arabidopsis thaliana <400> 12 Met Val Ser Met Leu Thr Asn Val Val Ser Gly Glu Thr Glu Pro Ser   1 5 10 15 Ala Ser Ala Thr Trp Thr Met Gly His Lys Arg Glu Arg Glu Glu Phe              20 25 30 Ser Leu Pro Pro Gln Pro Leu Ile Thr Gly Ser Ala Val Thr Lys Glu          35 40 45 Cys Glu Ser Ser Met Ser Leu Glu Arg Pro Lys Lys Tyr Arg Gly Val      50 55 60 Arg Gln Arg Pro Trp Gly Lys Trp Ala Ala Glu Ile Arg Asp Pro His  65 70 75 80 Lys Ala Thr Arg Val Trp Leu Gly Thr Phe Glu Thr Ala Glu Ala Ala                  85 90 95 Ala Arg Ala Tyr Asp Ala Ala Ala Leu Arg Phe Arg Gly Ser Lys Ala             100 105 110 Lys Leu Asn Phe Pro Glu Asn Val Gly Thr Gln Thr Ile Gln Arg Asn         115 120 125 Ser His Phe Leu Gln Asn Ser Met Gln Pro Ser Leu Thr Tyr Ile Asp     130 135 140 Gln Cys Pro Thr Leu Leu Ser Tyr Ser Arg Cys Met Glu Gln Gln Gln 145 150 155 160 Pro Leu Val Gly Met Leu Gln Pro Thr Glu Glu Glu Asn His Phe Phe                 165 170 175 Glu Lys Pro Trp Thr Glu Tyr Asp Gln Tyr Asn Tyr Ser Ser Phe Gly             180 185 190 <210> 13 <211> 1085 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (120) .. (953) <400> 13 acttcactct ctaatttcct tctctctatc tctcaccata ttcgcgatta aaaactctca 60 acttttctct caaatttctg atcctttgat ccaacagtta gaagaagatt catctgatc 119 atg gcc ctc gaa gcg atg aac act cca act tct tct ttc acc aga atc 167 Met Ala Leu Glu Ala Met Asn Thr Pro Thr Ser Ser Phe Thr Arg Ile   1 5 10 15 gaa acg aaa gaa gat ttg atg aac gac gcc gtt ttc att gag ccg tgg 215 Glu Thr Lys Glu Asp Leu Met Asn Asp Ala Val Phe Ile Glu Pro Trp              20 25 30 ctt aaa cgc aaa cgc tcc aaa cgt cag cgt tct cac agc cct tct tcg 263 Leu Lys Arg Lys Arg Ser Lys Arg Gln Arg Ser His Ser Pro Ser Ser          35 40 45 tct tct tcc tca ccg cct cga tct cga ccc aaa tcc cag aat caa gat 311 Ser Ser Ser Ser Pro Pro Arg Ser Arg Pro Lys Ser Gln Asn Gln Asp      50 55 60 ctt acg gaa gaa gag tat ctc gct ctt tgt ctc ctc atg ctc gct aaa 359 Leu Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Leu Met Leu Ala Lys  65 70 75 80 gat caa ccg tcg caa acg cga ttt cat caa cag tcg caa tcg tta acg 407 Asp Gln Pro Ser Gln Thr Arg Phe His Gln Gln Ser Gln Ser Leu Thr                  85 90 95 ccg ccg cca gaa tca aag aac ctt ccg tac aag tgt aac gtc tgt gaa 455 Pro Pro Pro Glu Ser Lys Asn Leu Pro Tyr Lys Cys Asn Val Cys Glu             100 105 110 aaa gcg ttt cct tcc tat cag gct tta ggc ggt cac aaa gca agt cac 503 Lys Ala Phe Pro Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His         115 120 125 cga atc aaa cca cca acc gta atc tca aca acc gcc gat gat tca aca 551 Arg Ile Lys Pro Pro Thr Val Ile Ser Thr Thr Ala Asp Asp Ser Thr     130 135 140 gct ccg acc atc tcc atc gtc gcc gga gaa aaa cat ccg att gct gcc 599 Ala Pro Thr Ile Ser Ile Val Ala Gly Glu Lys His Pro Ile Ala Ala 145 150 155 160 tcc gga aag atc cac gag tgt tca atc tgt cat aaa gtg ttt ccg acg 647 Ser Gly Lys Ile His Glu Cys Ser Ile Cys His Lys Val Phe Pro Thr                 165 170 175 ggt caa gct tta ggc ggt cac aaa cgt tgt cac tac gaa ggc aac ctc 695 Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu Gly Asn Leu             180 185 190 ggc ggc gga gga gga gga gga agc aaa tca atc agt cac agt gga agc 743 Gly Gly Gly Gly Gly Gly Gly Ser Lys Ser Ile Ser His Ser Gly Ser         195 200 205 gtg tcg agc acg gta tcg gaa gaa agg agc cac cgt gga ttc atc gat 791 Val Ser Ser Thr Val Ser Glu Glu Arg Ser His Arg Gly Phe Ile Asp     210 215 220 cta aac cta ccg gcg tta cct gaa ctc agc ctt cat cac aat cca atc 839 Leu Asn Leu Pro Ala Leu Pro Glu Leu Ser Leu His His Asn Pro Ile 225 230 235 240 gtc gac gaa gag atc ttg agt ccg ttg acc ggt aaa aaa acc gct ttt 887 Val Asp Glu Glu Ile Leu Ser Pro Leu Thr Gly Lys Lys Thr Ala Phe                 245 250 255 gtt gac cga tca cga cca agt cat caa gaa aga aga ttt atc ttt aaa 935 Val Asp Arg Ser Arg Pro Ser His Gln Glu Arg Arg Phe Ile Phe Lys             260 265 270 aat cta ata ctc gac tat taattcttgt gtgatttttt tcgttacaac 983 Asn Leu Ile Leu Asp Tyr         275 catagtttca ttttcatttt tttagttaca aatttttaat tgttctgatt tggattgaat 1043 attggtatat tgttaggggt tgatacaaaa aaaaaaaaaa aa 1085 <210> 14 <211> 278 <212> PRT <213> Arabidopsis thaliana <400> 14 Met Ala Leu Glu Ala Met Asn Thr Pro Thr Ser Ser Phe Thr Arg Ile   1 5 10 15 Glu Thr Lys Glu Asp Leu Met Asn Asp Ala Val Phe Ile Glu Pro Trp              20 25 30 Leu Lys Arg Lys Arg Ser Lys Arg Gln Arg Ser His Ser Pro Ser Ser          35 40 45 Ser Ser Ser Ser Pro Pro Arg Ser Arg Pro Lys Ser Gln Asn Gln Asp      50 55 60 Leu Thr Glu Glu Glu Tyr Leu Ala Leu Cys Leu Leu Met Leu Ala Lys  65 70 75 80 Asp Gln Pro Ser Gln Thr Arg Phe His Gln Gln Ser Gln Ser Leu Thr                  85 90 95 Pro Pro Pro Glu Ser Lys Asn Leu Pro Tyr Lys Cys Asn Val Cys Glu             100 105 110 Lys Ala Phe Pro Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His         115 120 125 Arg Ile Lys Pro Pro Thr Val Ile Ser Thr Thr Ala Asp Asp Ser Thr     130 135 140 Ala Pro Thr Ile Ser Ile Val Ala Gly Glu Lys His Pro Ile Ala Ala 145 150 155 160 Ser Gly Lys Ile His Glu Cys Ser Ile Cys His Lys Val Phe Pro Thr                 165 170 175 Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu Gly Asn Leu             180 185 190 Gly Gly Gly Gly Gly Gly Gly Ser Lys Ser Ile Ser His Ser Gly Ser         195 200 205 Val Ser Ser Thr Val Ser Glu Glu Arg Ser His Arg Gly Phe Ile Asp     210 215 220 Leu Asn Leu Pro Ala Leu Pro Glu Leu Ser Leu His His Asn Pro Ile 225 230 235 240 Val Asp Glu Glu Ile Leu Ser Pro Leu Thr Gly Lys Lys Thr Ala Phe                 245 250 255 Val Asp Arg Ser Arg Pro Ser His Gln Glu Arg Arg Phe Ile Phe Lys             260 265 270 Asn Leu Ile Leu Asp Tyr         275 <210> 15 <211> 1783 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (162) .. (1529) <400> 15 attgttaaaa gctctcacac aaccaccgtt ctccgtcacg gtggcgcttt attctctcat 60 cggagcgcct caccggtggc agacggtgtc gttgttcctc tcctaaaacc tccatcaatc 120 accatctctt tacacagagc tctaaccaaa atcttcgaga g atg ggg cag agt atg 176                                               Met Gly Gln Ser Met                                                 1 5 agc tgt gga agt cga ccg gag cac gga ata ttc gcc tct gta cag tgc 224 Ser Cys Gly Ser Arg Pro Glu His Gly Ile Phe Ala Ser Val Gln Cys                  10 15 20 ggc gat atc atc act atc cgt cgt gtg atg gcg acg gag cct agt ctg 272 Gly Asp Ile Ile Thr Ile Arg Arg Val Met Ala Thr Glu Pro Ser Leu              25 30 35 ttg aat caa act act cct tat gat cgt cac tct gtt ctt cat gtc gct 320 Leu Asn Gln Thr Thr Pro Tyr Asp Arg His Ser Val Leu His Val Ala          40 45 50 gct gct aat ggt cag atc gag att ttg tca ttg ctt ttg gaa cga ttt 368 Ala Ala Asn Gly Gln Ile Glu Ile Leu Ser Leu Leu Leu Glu Arg Phe      55 60 65 acg aat cca gat ttg ttg aat cgt cac aag cag act ccg tta atg ttg 416 Thr Asn Pro Asp Leu Leu Asn Arg His Lys Gln Thr Pro Leu Met Leu  70 75 80 85 gct gcg atg tat gga aga atc tct tgt gtg aag aag cta gct gaa gtt 464 Ala Ala Met Tyr Gly Arg Ile Ser Cys Val Lys Lys Leu Ala Glu Val                  90 95 100 gga gct aat att ttg atg ttt gat tct gtg aat cga aga aca tgt ttg 512 Gly Ala Asn Ile Leu Met Phe Asp Ser Val Asn Arg Arg Thr Cys Leu             105 110 115 cat tac gct gct tat tat gga cat gct aat tgt gtt caa gct att ctc 560 His Tyr Ala Ala Tyr Tyr Gly His Ala Asn Cys Val Gln Ala Ile Leu         120 125 130 tct gct gct caa tca agt cct gtt gct gtc cat tgg gga tat gcg aga 608 Ser Ala Ala Gln Ser Ser Pro Val Ala Val His Trp Gly Tyr Ala Arg     135 140 145 ttt gtg aac ata aga gat gat aaa gga gcg act ccg ttg cat tta gct 656 Phe Val Asn Ile Arg Asp Asp Lys Gly Ala Thr Pro Leu His Leu Ala 150 155 160 165 gct cga cag aga cga cct gaa tgt gtg aat gtt ttg ttg gat agt ggt 704 Ala Arg Gln Arg Arg Pro Glu Cys Val Asn Val Leu Leu Asp Ser Gly                 170 175 180 tct ctt gtt tgt gca tct act agt gta tat ggt tct cca gga agc aca 752 Ser Leu Val Cys Ala Ser Thr Ser Val Tyr Gly Ser Pro Gly Ser Thr             185 190 195 cct ctt cat tta gca gct aga agt gga tct ata gat tgt gtc aga aag 800 Pro Leu His Leu Ala Ala Arg Ser Gly Ser Ile Asp Cys Val Arg Lys         200 205 210 ttg ctt gct tgg ggt gct gat cgt ctt caa cga gac gct tct ggg aga 848 Leu Leu Ala Trp Gly Ala Asp Arg Leu Gln Arg Asp Ala Ser Gly Arg     215 220 225 ata cct tat gtg gtt gcg atg aag cat aag cat gga gca tgt gga gcc 896 Ile Pro Tyr Val Val Ala Met Lys His Lys His Gly Ala Cys Gly Ala 230 235 240 245 tta ctt aat ccg tcc tct gca gag cca ctt gtt tgg cca tca cca tta 944 Leu Leu Asn Pro Ser Ser Ala Glu Pro Leu Val Trp Pro Ser Pro Leu                 250 255 260 aag ttc atc agt gag ctt aat gac gag gcg aaa ctt ctc tta gag cag 992 Lys Phe Ile Ser Glu Leu Asn Asp Glu Ala Lys Leu Leu Leu Glu Gln             265 270 275 gct tta atg gag gct aac agg gag aga gag aaa acc atc ctc aaa gga 1040 Ala Leu Met Glu Ala Asn Arg Glu Arg Glu Lys Thr Ile Leu Lys Gly         280 285 290 aca gct tat tcc tta cca tca ccc tct ttc tct gac acg gat gat aac 1088 Thr Ala Tyr Ser Leu Pro Ser Pro Ser Phe Ser Asp Thr Asp Asp Asn     295 300 305 atg tcc gag gtg agt gat acg gaa ctg tgc tgc att tgc ttt gag caa 1136 Met Ser Glu Val Ser Asp Thr Glu Leu Cys Cys Ile Cys Phe Glu Gln 310 315 320 325 gta tgt aca att gaa gtt aaa gac tgt ggt cac caa atg tgt gca caa 1184 Val Cys Thr Ile Glu Val Lys Asp Cys Gly His Gln Met Cys Ala Gln                 330 335 340 tgc aca ctt gca ctg tgc tgt cac aac aaa cca aac cca acg acc tca 1232 Cys Thr Leu Ala Leu Cys Cys His Asn Lys Pro Asn Pro Thr Thr Ser             345 350 355 acc gtg act cca ccg gtc tgt ccg ttc tgt aga agc acc att gca tgt 1280 Thr Val Thr Pro Pro Val Cys Pro Phe Cys Arg Ser Thr Ile Ala Cys         360 365 370 tta gtc gtc gcc cag aac aac aac aac aac aac gaa aag agc aaa agc 1328 Leu Val Val Ala Gln Asn Asn Asn Asn Asn Asn Glu Lys Ser Lys Ser     375 380 385 cta gat gat gtt gtt gtt gtt gat cgt gag gca ggt gat gtt agc tcc 1376 Leu Asp Asp Val Val Val Val Asp Arg Glu Ala Gly Asp Val Ser Ser 390 395 400 405 tcc aaa ttc aga aaa cat aga aga tca ata aac ctt ggc gaa gaa agc 1424 Ser Lys Phe Arg Lys His Arg Arg Ser Ile Asn Leu Gly Glu Glu Ser                 410 415 420 agc agc ttc atg gga cta tca act att gga tca ttc ggt agg ata acc 1472 Ser Ser Phe Met Gly Leu Ser Thr Ile Gly Ser Phe Gly Arg Ile Thr             425 430 435 ggc cgt ggc tcg gga agg atc gca gcc gaa aac gag ctg atg gac aaa 1520 Gly Arg Gly Ser Gly Arg Ile Ala Ala Glu Asn Glu Leu Met Asp Lys         440 445 450 cca ata ttg tgagggatcg attccgtttt aagggacatt ttggggcatg 1569 Pro Ile Leu     455 ggggagcaat aaaaaagatg aggggatgaa attgtgagaa tgtataaaat atagatgaat 1629 ttatgttaga tcttttgttg aagggaggaa gattgaaata aggaaaaaga tgtggggagg 1689 tgtgtaatgc aaggatttgt tgtttctttg attaagtttg gccaaaattg tttgttgttg 1749 ttattatttg gttacttgat atgaaaggga aacc 1783 <210> 16 <211> 456 <212> PRT <213> Arabidopsis thaliana <400> 16 Met Gly Gln Ser Met Ser Cys Gly Ser Arg Pro Glu His Gly Ile Phe   1 5 10 15 Ala Ser Val Gln Cys Gly Asp Ile Ile Thr Ile Arg Arg Val Met Ala              20 25 30 Thr Glu Pro Ser Leu Leu Asn Gln Thr Thr Pro Tyr Asp Arg His Ser          35 40 45 Val Leu His Val Ala Ala Ala Asn Gly Gln Ile Glu Ile Leu Ser Leu      50 55 60 Leu Leu Glu Arg Phe Thr Asn Pro Asp Leu Leu Asn Arg His Lys Gln  65 70 75 80 Thr Pro Leu Met Leu Ala Ala Met Tyr Gly Arg Ile Ser Cys Val Lys                  85 90 95 Lys Leu Ala Glu Val Gly Ala Asn Ile Leu Met Phe Asp Ser Val Asn             100 105 110 Arg Arg Thr Cys Leu His Tyr Ala Ala Tyr Tyr Gly His Ala Asn Cys         115 120 125 Val Gln Ala Ile Leu Ser Ala Ala Gln Ser Ser Pro Val Ala Val His     130 135 140 Trp Gly Tyr Ala Arg Phe Val Asn Ile Arg Asp Asp Lys Gly Ala Thr 145 150 155 160 Pro Leu His Leu Ala Ala Arg Gln Arg Arg Pro Glu Cys Val Asn Val                 165 170 175 Leu Leu Asp Ser Gly Ser Leu Val Cys Ala Ser Thr Ser Val Tyr Gly             180 185 190 Ser Pro Gly Ser Thr Pro Leu His Leu Ala Ala Arg Ser Gly Ser Ile         195 200 205 Asp Cys Val Arg Lys Leu Leu Ala Trp Gly Ala Asp Arg Leu Gln Arg     210 215 220 Asp Ala Ser Gly Arg Ile Pro Tyr Val Val Ala Met Lys His Lys His 225 230 235 240 Gly Ala Cys Gly Ala Leu Leu Asn Pro Ser Ser Ala Glu Pro Leu Val                 245 250 255 Trp Pro Ser Pro Leu Lys Phe Ile Ser Glu Leu Asn Asp Glu Ala Lys             260 265 270 Leu Leu Leu Glu Gln Ala Leu Met Glu Ala Asn Arg Glu Arg Glu Lys         275 280 285 Thr Ile Leu Lys Gly Thr Ala Tyr Ser Leu Pro Ser Pro Ser Phe Ser     290 295 300 Asp Thr Asp Asp Asn Met Ser Glu Val Ser Asp Thr Glu Leu Cys Cys 305 310 315 320 Ile Cys Phe Glu Gln Val Cys Thr Ile Glu Val Lys Asp Cys Gly His                 325 330 335 Gln Met Cys Ala Gln Cys Thr Leu Ala Leu Cys Cys His Asn Lys Pro             340 345 350 Asn Pro Thr Thr Ser Thr Val Thr Pro Pro Val Cys Pro Phe Cys Arg         355 360 365 Ser Thr Ile Ala Cys Leu Val Val Ala Gln Asn Asn Asn Asn Asn Asn     370 375 380 Glu Lys Ser Lys Ser Leu Asp Asp Val Val Val Val Asp Arg Glu Ala 385 390 395 400 Gly Asp Val Ser Ser Ser Lys Phe Arg Lys His Arg Arg Ser Ile Asn                 405 410 415 Leu Gly Glu Glu Ser Ser Ser Phe Met Gly Leu Ser Thr Ile Gly Ser             420 425 430 Phe Gly Arg Ile Thr Gly Arg Gly Ser Gly Arg Ile Ala Ala Glu Asn         435 440 445 Glu Leu Met Asp Lys Pro Ile Leu     450 455 <210> 17 <211> 714 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (711) <400> 17 atg aag ata caa tgt gat gtg tgt gag aaa gct ccg gcc acg ctt ata 48 Met Lys Ile Gln Cys Asp Val Cys Glu Lys Ala Pro Ala Thr Leu Ile   1 5 10 15 tgt tgt gct gat gaa gct gct ctc tgc gct aaa tgt gac gtt gag gtt 96 Cys Cys Ala Asp Glu Ala Ala Leu Cys Ala Lys Cys Asp Val Glu Val              20 25 30 cat gct gct aat aaa ctc gct agc aaa cac caa cgc ctt ttt ctt gac 144 His Ala Ala Asn Lys Leu Ala Ser Lys His Gln Arg Leu Phe Leu Asp          35 40 45 tct ctc tca act aaa ttc cct ccc tgc gac atc tgc ctt gag aag gca 192 Ser Leu Ser Thr Lys Phe Pro Pro Cys Asp Ile Cys Leu Glu Lys Ala      50 55 60 gct ttc ata ttc tgt gta gag gat agg gct ctg ctc tgc aga gat tgc 240 Ala Phe Ile Phe Cys Val Glu Asp Arg Ala Leu Leu Cys Arg Asp Cys  65 70 75 80 gat gag gcg acc cat gcg cca aat act cgc tct gct aat cac cag agg 288 Asp Glu Ala Thr His Ala Pro Asn Thr Arg Ser Ala Asn His Gln Arg                  85 90 95 ttc tta gcc act gga atc cga gtt gct ctt agt tcc act agt tgc aat 336 Phe Leu Ala Thr Gly Ile Arg Val Ala Leu Ser Ser Thr Ser Cys Asn             100 105 110 caa gaa gtg gaa aag aat cac ttt gac cca tct aat cag cag agt ctc 384 Gln Glu Val Glu Lys Asn His Phe Asp Pro Ser Asn Gln Gln Ser Leu         115 120 125 tct aaa ccg cca act cag caa ccc gct gct cca tct cct ttg tgg gct 432 Ser Lys Pro Pro Thr Gln Gln Pro Ala Ala Pro Ser Pro Leu Trp Ala     130 135 140 acc gat gaa ttc ttc agc tac tct gat ctt gac tgc agt aat aag aaa 480 Thr Asp Glu Phe Phe Ser Tyr Ser Asp Leu Asp Cys Ser Asn Lys Lys 145 150 155 160 gag caa ctc gat ctc ggg gag ctg gat tgg ctt gca gag atg ggt ctg 528 Glu Gln Leu Asp Leu Gly Glu Leu Asp Trp Leu Ala Glu Met Gly Leu                 165 170 175 ttt ggt gac cag cct gat caa gag gct cta ccg gta gcc gaa gtt ccc 576 Phe Gly Asp Gln Pro Asp Gln Glu Ala Leu Pro Val Ala Glu Val Pro             180 185 190 gag ctt tcc ttt tca cat ttg gct cat gct cat tcc tac aac aga cct 624 Glu Leu Ser Phe Ser His Leu Ala His Ala His Ser Tyr Asn Arg Pro         195 200 205 atg aag tcc aat gta ccc aac aag aag cag agg ctt gag tac cgg tat 672 Met Lys Ser Asn Val Pro Asn Lys Lys Gln Arg Leu Glu Tyr Arg Tyr     210 215 220 gat gat gaa gaa gag cac ttc cta gtc ccc gac cta ggc taa 714 Asp Asp Glu Glu Glu His Phe Leu Val Pro Asp Leu Gly 225 230 235 <210> 18 <211> 237 <212> PRT <213> Arabidopsis thaliana <400> 18 Met Lys Ile Gln Cys Asp Val Cys Glu Lys Ala Pro Ala Thr Leu Ile   1 5 10 15 Cys Cys Ala Asp Glu Ala Ala Leu Cys Ala Lys Cys Asp Val Glu Val              20 25 30 His Ala Ala Asn Lys Leu Ala Ser Lys His Gln Arg Leu Phe Leu Asp          35 40 45 Ser Leu Ser Thr Lys Phe Pro Pro Cys Asp Ile Cys Leu Glu Lys Ala      50 55 60 Ala Phe Ile Phe Cys Val Glu Asp Arg Ala Leu Leu Cys Arg Asp Cys  65 70 75 80 Asp Glu Ala Thr His Ala Pro Asn Thr Arg Ser Ala Asn His Gln Arg                  85 90 95 Phe Leu Ala Thr Gly Ile Arg Val Ala Leu Ser Ser Thr Ser Cys Asn             100 105 110 Gln Glu Val Glu Lys Asn His Phe Asp Pro Ser Asn Gln Gln Ser Leu         115 120 125 Ser Lys Pro Pro Thr Gln Gln Pro Ala Ala Pro Ser Pro Leu Trp Ala     130 135 140 Thr Asp Glu Phe Phe Ser Tyr Ser Asp Leu Asp Cys Ser Asn Lys Lys 145 150 155 160 Glu Gln Leu Asp Leu Gly Glu Leu Asp Trp Leu Ala Glu Met Gly Leu                 165 170 175 Phe Gly Asp Gln Pro Asp Gln Glu Ala Leu Pro Val Ala Glu Val Pro             180 185 190 Glu Leu Ser Phe Ser His Leu Ala His Ala His Ser Tyr Asn Arg Pro         195 200 205 Met Lys Ser Asn Val Pro Asn Lys Lys Gln Arg Leu Glu Tyr Arg Tyr     210 215 220 Asp Asp Glu Glu Glu His Phe Leu Val Pro Asp Leu Gly 225 230 235 <210> 19 <211> 829 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (99) .. (584) <400> 19 atcacaacta ctatcacacc aaactcaaaa aacacaaacc acaagaggat catttcattt 60 tttattgttt cgttttaatc atcatcatca gaagaaaa atg gtt gcg ata tcg gag 116                                           Met Val Ala Ile Ser Glu                                             1 5 atc aag tcg acg gtg gat gtc acg gcg gcg aat tgt ttg atg ctt tta 164 Ile Lys Ser Thr Val Asp Val Thr Ala Ala Asn Cys Leu Met Leu Leu              10 15 20 tct aga gtt gga caa gaa aac gtt gac ggt ggc gat caa aaa cgc gtt 212 Ser Arg Val Gly Gln Glu Asn Val Asp Gly Gly Asp Gln Lys Arg Val          25 30 35 ttc aca tgt aaa acg tgt ttg aag cag ttt cat tcg ttc caa gcc tta 260 Phe Thr Cys Lys Thr Cys Leu Lys Gln Phe His Ser Phe Gln Ala Leu      40 45 50 gga ggt cac cgt gcg agt cac aag aag cct aac aac gac gct ttg tcg 308 Gly Gly His Arg Ala Ser His Lys Lys Pro Asn Asn Asp Ala Leu Ser  55 60 65 70 tct gga ttg atg aag aag gtg aaa acg tcg tcg cat cct tgt ccc ata 356 Ser Gly Leu Met Lys Lys Val Lys Thr Ser Ser His Pro Cys Pro Ile                  75 80 85 tgt gga gtg gag ttt ccg atg gga caa gct ttg gga gga cac atg agg 404 Cys Gly Val Glu Phe Pro Met Gly Gln Ala Leu Gly Gly His Met Arg              90 95 100 aga cac agg aac gag agt ggg gct gct ggt ggc gcg ttg gtt aca cgc 452 Arg His Arg Asn Glu Ser Gly Ala Ala Gly Gly Ala Leu Val Thr Arg         105 110 115 gct ttg ttg ccg gag ccc acg gtg act acg ttg aag aaa tct agc agt 500 Ala Leu Leu Pro Glu Pro Thr Val Thr Thr Leu Lys Lys Ser Ser Ser     120 125 130 ggg aag aga gtg gct tgt ttg gat ctg agt cta ggg atg gtg gac aat 548 Gly Lys Arg Val Ala Cys Leu Asp Leu Ser Leu Gly Met Val Asp Asn 135 140 145 150 ttg aat ctc aag ttg gag ctt gga aga aca gtt tat tgattttatt 594 Leu Asn Leu Lys Leu Glu Leu Gly Arg Thr Val Tyr                 155 160 tattttcctt aaattttctg aatatatttg tttctctcat tctttgaatt tttcttaata 654 ttctagatta tacatacatc cgcagattta ggaaactttc atagagtgta atcttttctt 714 tctgtaaaaa tatattttac ttgtagcatt ggagatttgt tatgagatta tcttacttag 774 catttagtga ataatctatt agcctatttt gccgacgcga aaaaaaaaaa aaaaa 829 <210> 20 <211> 162 <212> PRT <213> Arabidopsis thaliana <400> 20 Met Val Ala Ile Ser Glu Ile Lys Ser Thr Val Asp Val Thr Ala Ala   1 5 10 15 Asn Cys Leu Met Leu Leu Ser Arg Val Gly Gln Glu Asn Val Asp Gly              20 25 30 Gly Asp Gln Lys Arg Val Phe Thr Cys Lys Thr Cys Leu Lys Gln Phe          35 40 45 His Ser Phe Gln Ala Leu Gly Gly His Arg Ala Ser His Lys Lys Pro      50 55 60 Asn Asn Asp Ala Leu Ser Ser Gly Leu Met Lys Lys Val Lys Thr Ser  65 70 75 80 Ser His Pro Cys Pro Ile Cys Gly Val Glu Phe Pro Met Gly Gln Ala                  85 90 95 Leu Gly Gly His Met Arg Arg His Arg Asn Glu Ser Gly Ala Ala Gly             100 105 110 Gly Ala Leu Val Thr Arg Ala Leu Leu Pro Glu Pro Thr Val Thr Thr         115 120 125 Leu Lys Lys Ser Ser Ser Gly Lys Arg Val Ala Cys Leu Asp Leu Ser     130 135 140 Leu Gly Met Val Asp Asn Leu Asn Leu Lys Leu Glu Leu Gly Arg Thr 145 150 155 160 Val Tyr <210> 21 <211> 881 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (89) .. (769) <400> 21 agcaattaaa caatttcttc actgcaattc acaagcaacc ttcaaactaa aactcgagag 60 acaagaaatc ctcagaatct ttaactta atg gcg ctc gag gct ctt aca tca 112                                Met Ala Leu Glu Ala Leu Thr Ser                                  1 5 cca aga tta gct tct ccg att cct cct ttg ttc gaa gat tct tca gtc 160 Pro Arg Leu Ala Ser Pro Ile Pro Pro Leu Phe Glu Asp Ser Ser Val      10 15 20 ttc cat gga gtc gag cac tgg aca aag ggt aag cga tct aag aga tca 208 Phe His Gly Val Glu His Trp Thr Lys Gly Lys Arg Ser Lys Arg Ser  25 30 35 40 aga tcc gat ttc cac cac caa aac ctc act gag gaa gag tat cta gct 256 Arg Ser Asp Phe His His Gln Asn Leu Thr Glu Glu Glu Tyr Leu Ala                  45 50 55 ttt tgc ctc atg ctt ctc gct cgc gac aac cgt cag cct cct cct cct 304 Phe Cys Leu Met Leu Leu Ala Arg Asp Asn Arg Gln Pro Pro Pro Pro              60 65 70 ccg gcg gtg gag aag ttg agc tac aag tgt agc gtc tgc gac aag acg 352 Pro Ala Val Glu Lys Leu Ser Tyr Lys Cys Ser Val Cys Asp Lys Thr          75 80 85 ttc tct tct tac caa gct ctc ggt ggt cac aag gca agc cac cgt aag 400 Phe Ser Ser Tyr Gln Ala Leu Gly Gly His Lys Ala Ser His Arg Lys      90 95 100 aac tta tca cag act ctc tcc ggc gga gga gat gat cat tca acc tcg 448 Asn Leu Ser Gln Thr Leu Ser Gly Gly Gly Asp Asp His Ser Thr Ser 105 110 115 120 tcg gcg aca acc aca tcc gcc gtg act act gga agt ggg aaa tca cac 496 Ser Ala Thr Thr Thr Ser Ala Val Thr Thr Gly Ser Gly Lys Ser His                 125 130 135 gtt tgc acc atc tgt aac aag tct ttt cct tcc ggt caa gct ctc ggc 544 Val Cys Thr Ile Cys Asn Lys Ser Phe Pro Ser Gly Gln Ala Leu Gly             140 145 150 gga cac aag cgg tgc cac tac gaa gga aac aac aac atc aac act agt 592 Gly His Lys Arg Cys His Tyr Glu Gly Asn Asn Asn Ile Asn Thr Ser         155 160 165 agc gtg tcc aac tcc gaa ggt gcg ggg tcc act agc cac gtt agc agt 640 Ser Val Ser Asn Ser Glu Gly Ala Gly Ser Thr Ser His Val Ser Ser     170 175 180 agc cac cgt ggg ttt gac ctc aac atc cct ccg atc cct gaa ttc tcg 688 Ser His Arg Gly Phe Asp Leu Asn Ile Pro Pro Ile Pro Glu Phe Ser 185 190 195 200 atg gtc aac gga gac gac gaa gtc atg agc cct atg ccg gcg aag aag 736 Met Val Asn Gly Asp Asp Glu Val Met Ser Pro Met Pro Ala Lys Lys                 205 210 215 cct cgg ttt gac ttt ccg gtc aaa ctt caa ctt taaggaaatt tacttagacg 789 Pro Arg Phe Asp Phe Pro Val Lys Leu Gln Leu             220 225 ataagatttc gtttgtatac tgttgagagt tgtgtaggaa tttgttgact gtacatacca 849 aattggactt tgactcaaaa aaaaaaaaaa aa 881 <210> 22 <211> 227 <212> PRT <213> Arabidopsis thaliana <400> 22 Met Ala Leu Glu Ala Leu Thr Ser Pro Arg Leu Ala Ser Pro Ile Pro   1 5 10 15 Pro Leu Phe Glu Asp Ser Ser Val Phe His Gly Val Glu His Trp Thr              20 25 30 Lys Gly Lys Arg Ser Lys Arg Ser Arg Ser Asp Phe His His Gln Asn          35 40 45 Leu Thr Glu Glu Glu Tyr Leu Ala Phe Cys Leu Met Leu Leu Ala Arg      50 55 60 Asp Asn Arg Gln Pro Pro Pro Pro Pro Ala Val Glu Lys Leu Ser Tyr  65 70 75 80 Lys Cys Ser Val Cys Asp Lys Thr Phe Ser Ser Tyr Gln Ala Leu Gly                  85 90 95 Gly His Lys Ala Ser His Arg Lys Asn Leu Ser Gln Thr Leu Ser Gly             100 105 110 Gly Gly Asp Asp His Ser Thr Ser Ser Ala Thr Thr Thr Ser Ala Val         115 120 125 Thr Thr Gly Ser Gly Lys Ser His Val Cys Thr Ile Cys Asn Lys Ser     130 135 140 Phe Pro Ser Gly Gln Ala Leu Gly Gly His Lys Arg Cys His Tyr Glu 145 150 155 160 Gly Asn Asn Asn Ile Asn Thr Ser Ser Val Ser Asn Ser Glu Gly Ala                 165 170 175 Gly Ser Thr Ser His Val Ser Ser Ser His Arg Gly Phe Asp Leu Asn             180 185 190 Ile Pro Pro Ile Pro Glu Phe Ser Met Val Asn Gly Asp Asp Glu Val         195 200 205 Met Ser Pro Met Pro Ala Lys Lys Pro Arg Phe Asp Phe Pro Val Lys     210 215 220 Leu Gln Leu 225 <210> 23 <211> 1107 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (139) .. (957) <400> 23 aactcggtcc gagtcgactc ggtccgacaa gcgattttcg tgactccgtc gtcaccacgc 60 ctatgttaag tgagtaaatc cgactcttct ccgtagagat ttctcctact ttcaggctct 120 cttgtgaatt tggaagac atg agc ttt gtt ttc cgg gga agt aga gga gat 171                     Met Ser Phe Val Phe Arg Gly Ser Arg Gly Asp                       1 5 10 tta gaa agc gga ttc tcg ggt ggt ttt cta ccc gaa aga cga gct atg 219 Leu Glu Ser Gly Phe Ser Gly Gly Phe Leu Pro Glu Arg Arg Ala Met              15 20 25 cgt gtt cat gga gct cga cca gtt aat tct aat tcc ctc gct ttt ctg 267 Arg Val His Gly Ala Arg Pro Val Asn Ser Asn Ser Leu Ala Phe Leu          30 35 40 gtt aca gtt ctt ttg ctg ttt atg att ctc aat tcg cat cag atg cct 315 Val Thr Val Leu Leu Leu Phe Met Ile Leu Asn Ser His Gln Met Pro      45 50 55 cct aat ttc ctg ctg tgg ctt gtg ctt ggg gtg ttt ttg atg gca acg 363 Pro Asn Phe Leu Leu Trp Leu Val Leu Gly Val Phe Leu Met Ala Thr  60 65 70 75 acg ctt agg atg tat gct act tgc caa caa ctt caa gct cat gct cag 411 Thr Leu Arg Met Tyr Ala Thr Cys Gln Gln Leu Gln Ala His Ala Gln                  80 85 90 gct cag gct gca gca gca agt ggc ctc ttt agc cat act gag ctg agg 459 Ala Gln Ala Ala Ala Ala Ser Gly Leu Phe Ser His Thr Glu Leu Arg              95 100 105 ttg cat gtg cct cct tcc att gct ctt gct acg aga ggg cgt ctt cag 507 Leu His Val Pro Pro Ser Ile Ala Leu Ala Thr Arg Gly Arg Leu Gln         110 115 120 gga ctt agg ctc cag ctg gct ctt ctt gat cgg gaa ttt gat gac tta 555 Gly Leu Arg Leu Gln Leu Ala Leu Leu Asp Arg Glu Phe Asp Asp Leu     125 130 135 gat tat gaa act tta aga gca ctt gat tct gat aat gtt tcc aca act 603 Asp Tyr Glu Thr Leu Arg Ala Leu Asp Ser Asp Asn Val Ser Thr Thr 140 145 150 155 tct atg agc gag gaa gag ata aat gca ctt cca gtt cac aag tac aag 651 Ser Met Ser Glu Glu Glu Ile Asn Ala Leu Pro Val His Lys Tyr Lys                 160 165 170 gtg ttg gat cct gaa aat ggt tgc tct ttg gca aag caa gcg tca acc 699 Val Leu Asp Pro Glu Asn Gly Cys Ser Leu Ala Lys Gln Ala Ser Thr             175 180 185 tca tcc tca gct gag aag atg cta gat tct gcc aat gaa agt aaa aaa 747 Ser Ser Ser Ala Glu Lys Met Leu Asp Ser Ala Asn Glu Ser Lys Lys         190 195 200 gga aca gaa gat gag ctc aca tgt agt gtt tgc cta gaa caa gtt acc 795 Gly Thr Glu Asp Glu Leu Thr Cys Ser Val Cys Leu Glu Gln Val Thr     205 210 215 gta ggg gaa atc gtt cgc acc tta cct tgt ttg cat cag ttt cat gca 843 Val Gly Glu Ile Val Arg Thr Leu Pro Cys Leu His Gln Phe His Ala 220 225 230 235 gga tgt atc gat cca tgg ttg aga cag caa gga aca tgt cct gtc tgt 891 Gly Cys Ile Asp Pro Trp Leu Arg Gln Gln Gly Thr Cys Pro Val Cys                 240 245 250 aaa ttt aga gct cat tca gga tgg caa gaa caa gat gag att gat gat 939 Lys Phe Arg Ala His Ser Gly Trp Gln Glu Gln Asp Glu Ile Asp Asp             255 260 265 gat gct tcc gac atg gtt tgaaaagttt ggtttcgaac acttgtttat 987 Asp Ala Ser Asp Met Val         270 gttattaatg tgcgtgcgaa ttaaacccca aacaaatcat gtaatggtca ataactcaat 1047 atgatgtaac tgatgactct ccctttccaa aagtttgatt tagattcatc atgaaaaagt 1107 <210> 24 <211> 273 <212> PRT <213> Arabidopsis thaliana <400> 24 Met Ser Phe Val Phe Arg Gly Ser Arg Gly Asp Leu Glu Ser Gly Phe   1 5 10 15 Ser Gly Gly Phe Leu Pro Glu Arg Arg Ala Met Arg Val His Gly Ala              20 25 30 Arg Pro Val Asn Ser Asn Ser Leu Ala Phe Leu Val Thr Val Leu Leu          35 40 45 Leu Phe Met Ile Leu Asn Ser His Gln Met Pro Pro Asn Phe Leu Leu      50 55 60 Trp Leu Val Leu Gly Val Phe Leu Met Ala Thr Thr Leu Arg Met Tyr  65 70 75 80 Ala Thr Cys Gln Gln Leu Gln Ala His Ala Gln Ala Gln Ala Ala Ala                  85 90 95 Ala Ser Gly Leu Phe Ser His Thr Glu Leu Arg Leu His Val Pro Pro             100 105 110 Ser Ile Ala Leu Ala Thr Arg Gly Arg Leu Gln Gly Leu Arg Leu Gln         115 120 125 Leu Ala Leu Leu Asp Arg Glu Phe Asp Asp Leu Asp Tyr Glu Thr Leu     130 135 140 Arg Ala Leu Asp Ser Asp Asn Val Ser Thr Thr Ser Met Ser Glu Glu 145 150 155 160 Glu Ile Asn Ala Leu Pro Val His Lys Tyr Lys Val Leu Asp Pro Glu                 165 170 175 Asn Gly Cys Ser Leu Ala Lys Gln Ala Ser Thr Ser Ser Ser Ala Glu             180 185 190 Lys Met Leu Asp Ser Ala Asn Glu Ser Lys Lys Gly Thr Glu Asp Glu         195 200 205 Leu Thr Cys Ser Val Cys Leu Glu Gln Val Thr Val Gly Glu Ile Val     210 215 220 Arg Thr Leu Pro Cys Leu His Gln Phe His Ala Gly Cys Ile Asp Pro 225 230 235 240 Trp Leu Arg Gln Gln Gly Thr Cys Pro Val Cys Lys Phe Arg Ala His                 245 250 255 Ser Gly Trp Gln Glu Gln Asp Glu Ile Asp Asp Asp Ala Ser Asp Met             260 265 270 Val <210> 25 <211> 531 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (528) <400> 25 atg gga tcg gaa caa aac gat agc aca agc ttc acg caa tcg caa gct 48 Met Gly Ser Glu Gln Asn Asp Ser Thr Ser Phe Thr Gln Ser Gln Ala   1 5 10 15 tca gag cca aag cta tgt gtt aaa gga tgt ggt ttc ttt gga tca cca 96 Ser Glu Pro Lys Leu Cys Val Lys Gly Cys Gly Phe Phe Gly Ser Pro              20 25 30 tca aac atg gat ctc tgt tct aaa tgt tac aga ggc att tgt gct gag 144 Ser Asn Met Asp Leu Cys Ser Lys Cys Tyr Arg Gly Ile Cys Ala Glu          35 40 45 gaa gct caa aca gca gtt gct aaa gct gct gtt gaa aaa tct ttc aag 192 Glu Ala Gln Thr Ala Val Ala Lys Ala Ala Val Glu Lys Ser Phe Lys      50 55 60 cct tct cct cct cgt agt ctc ttc ata gca gaa cct cct gct gtt gtt 240 Pro Ser Pro Pro Arg Ser Leu Phe Ile Ala Glu Pro Pro Ala Val Val  65 70 75 80 gtg gaa ccc aaa ccc gaa aag gcg gca gtt gtt gtt gtc tcg gcc gag 288 Val Glu Pro Lys Pro Glu Lys Ala Ala Val Val Val Val Ser Ala Glu                  85 90 95 cca tct tcc tcg gcg gtt cct gag gcg aac gag cca tcg aga cct gca 336 Pro Ser Ser Ser Ala Val Pro Glu Ala Asn Glu Pro Ser Arg Pro Ala             100 105 110 cga acc aac cgg tgt ttg tgt tgt aac aag aag gtt ggg atc atg ggg 384 Arg Thr Asn Arg Cys Leu Cys Cys Asn Lys Lys Val Gly Ile Met Gly         115 120 125 ttt aag tgc aaa tgc ggg agc act ttc tgc ggc gaa cat cgg tac ccg 432 Phe Lys Cys Lys Cys Gly Ser Thr Phe Cys Gly Glu His Arg Tyr Pro     130 135 140 gag act cat gat tgc agc ttt gat ttc aaa gaa gtt gga cgt gga gag 480 Glu Thr His Asp Cys Ser Phe Asp Phe Lys Glu Val Gly Arg Gly Glu 145 150 155 160 att gcc aaa gct aat cct gtg gtt aag gct gat aaa att caa agg ttc 528 Ile Ala Lys Ala Asn Pro Val Val Lys Ala Asp Lys Ile Gln Arg Phe                 165 170 175 tga 531 <210> 26 <211> 176 <212> PRT <213> Arabidopsis thaliana <400> 26 Met Gly Ser Glu Gln Asn Asp Ser Thr Ser Phe Thr Gln Ser Gln Ala   1 5 10 15 Ser Glu Pro Lys Leu Cys Val Lys Gly Cys Gly Phe Phe Gly Ser Pro              20 25 30 Ser Asn Met Asp Leu Cys Ser Lys Cys Tyr Arg Gly Ile Cys Ala Glu          35 40 45 Glu Ala Gln Thr Ala Val Ala Lys Ala Ala Val Glu Lys Ser Phe Lys      50 55 60 Pro Ser Pro Pro Arg Ser Leu Phe Ile Ala Glu Pro Pro Ala Val Val  65 70 75 80 Val Glu Pro Lys Pro Glu Lys Ala Ala Val Val Val Val Ser Ala Glu                  85 90 95 Pro Ser Ser Ser Ala Val Pro Glu Ala Asn Glu Pro Ser Arg Pro Ala             100 105 110 Arg Thr Asn Arg Cys Leu Cys Cys Asn Lys Lys Val Gly Ile Met Gly         115 120 125 Phe Lys Cys Lys Cys Gly Ser Thr Phe Cys Gly Glu His Arg Tyr Pro     130 135 140 Glu Thr His Asp Cys Ser Phe Asp Phe Lys Glu Val Gly Arg Gly Glu 145 150 155 160 Ile Ala Lys Ala Asn Pro Val Val Lys Ala Asp Lys Ile Gln Arg Phe                 165 170 175 <210> 27 <211> 1068 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (1065) <400> 27 atg ttg aaa gta gag agt aac tgg gca caa gcc tgt gat aca tgc cga 48 Met Leu Lys Val Glu Ser Asn Trp Ala Gln Ala Cys Asp Thr Cys Arg   1 5 10 15 tca gcc gcc tgc acc gtg tac tgc cgg gct gat tct gcc tac ttg tgc 96 Ser Ala Ala Cys Thr Val Tyr Cys Arg Ala Asp Ser Ala Tyr Leu Cys              20 25 30 tcc agt tgt gat gct caa gtt cat gct gcc aat cgt ctt gct tcc cgc 144 Ser Ser Cys Asp Ala Gln Val His Ala Ala Asn Arg Leu Ala Ser Arg          35 40 45 cat gaa cgt gtt cga gtc tgt caa tca tgt gag cga gcc ccg gct gcc 192 His Glu Arg Val Arg Val Cys Gln Ser Cys Glu Arg Ala Pro Ala Ala      50 55 60 ttt ttc tgc aag gca gat gct gca tct cta tgc aca acc tgt gat tca 240 Phe Phe Cys Lys Ala Asp Ala Ala Ser Leu Cys Thr Thr Cys Asp Ser  65 70 75 80 gag att cat tcc gca aac cca ctt gct aga cgc cat caa cga gtt cca 288 Glu Ile His Ser Ala Asn Pro Leu Ala Arg Arg His Gln Arg Val Pro                  85 90 95 att ctg ccc att tct gag tac tct tac agt tcc acg gcc act aac cat 336 Ile Leu Pro Ile Ser Glu Tyr Ser Tyr Ser Ser Thr Ala Thr Asn His             100 105 110 tca tgt gag aca aca gtg aca gat cca gag aac aga ctt gtg ctt ggt 384 Ser Cys Glu Thr Thr Val Thr Asp Pro Glu Asn Arg Leu Val Leu Gly         115 120 125 caa gaa gaa gag gat gaa gat gaa gca gag gcg gct tca tgg ttg ttg 432 Gln Glu Glu Glu Asp Glu Asp Glu Ala Glu Ala Ala Ser Trp Leu Leu     130 135 140 cct aat tca ggg aaa aac agt ggt aac aac aat ggc ttc tcg att ggg 480 Pro Asn Ser Gly Lys Asn Ser Gly Asn Asn Asn Gly Phe Ser Ile Gly 145 150 155 160 gat gag ttt ctg aac ctt gtt gat tat agt tcg agt gat aag caa ttc 528 Asp Glu Phe Leu Asn Leu Val Asp Tyr Ser Ser Ser Asp Lys Gln Phe                 165 170 175 aca gat caa tcc aat cag tat caa cta gac tgc aac gta cct cag agg 576 Thr Asp Gln Ser Asn Gln Tyr Gln Leu Asp Cys Asn Val Pro Gln Arg             180 185 190 agc tat ggg gaa gat gga gtt gtt cca ctt caa att gaa gta tca aag 624 Ser Tyr Gly Glu Asp Gly Val Val Pro Leu Gln Ile Glu Val Ser Lys         195 200 205 ggc atg tac caa gag caa cag aac ttt cag ctg agt atc aac tgt ggc 672 Gly Met Tyr Gln Glu Gln Gln Asn Phe Gln Leu Ser Ile Asn Cys Gly     210 215 220 tcc tgg gga gct ctt cga agc tcc aat ggt tcc ctc agt cat atg gtg 720 Ser Trp Gly Ala Leu Arg Ser Ser Asn Gly Ser Leu Ser His Met Val 225 230 235 240 aat gtt tca tct atg gac ctg gga gtt gtg ccg gag tca aca acg agt 768 Asn Val Ser Ser Met Asp Leu Gly Val Val Pro Glu Ser Thr Thr Ser                 245 250 255 gac gca aca gta tca aac cca aga tcg ccc aaa gcg gta aca gac caa 816 Asp Ala Thr Val Ser Asn Pro Arg Ser Pro Lys Ala Val Thr Asp Gln             260 265 270 cca cct tac cct cca gct cag atg ctc agt cca agg gac aga gaa gct 864 Pro Pro Tyr Pro Pro Ala Gln Met Leu Ser Pro Arg Asp Arg Glu Ala         275 280 285 aga gtc ctg aga tac aga gag aag aag aag atg agg aaa ttt gag aag 912 Arg Val Leu Arg Tyr Arg Glu Lys Lys Lys Met Arg Lys Phe Glu Lys     290 295 300 acg ata aga tat gct tca agg aaa gcg tat gca gag aaa aga cca cgg 960 Thr Ile Arg Tyr Ala Ser Arg Lys Ala Tyr Ala Glu Lys Arg Pro Arg 305 310 315 320 atc aag ggc cgg ttt gca aag aag aaa gat gtc gat gaa gag gca aac 1008 Ile Lys Gly Arg Phe Ala Lys Lys Lys Asp Val Asp Glu Glu Ala Asn                 325 330 335 caa gct ttc tcc aca atg ata aca ttt gac acc gga tat gga att gtt 1056 Gln Ala Phe Ser Thr Met Ile Thr Phe Asp Thr Gly Tyr Gly Ile Val             340 345 350 cca tca ttc tga 1068 Pro Ser Phe         355 <210> 28 <211> 355 <212> PRT <213> Arabidopsis thaliana <400> 28 Met Leu Lys Val Glu Ser Asn Trp Ala Gln Ala Cys Asp Thr Cys Arg   1 5 10 15 Ser Ala Ala Cys Thr Val Tyr Cys Arg Ala Asp Ser Ala Tyr Leu Cys              20 25 30 Ser Ser Cys Asp Ala Gln Val His Ala Ala Asn Arg Leu Ala Ser Arg          35 40 45 His Glu Arg Val Arg Val Cys Gln Ser Cys Glu Arg Ala Pro Ala Ala      50 55 60 Phe Phe Cys Lys Ala Asp Ala Ala Ser Leu Cys Thr Thr Cys Asp Ser  65 70 75 80 Glu Ile His Ser Ala Asn Pro Leu Ala Arg Arg His Gln Arg Val Pro                  85 90 95 Ile Leu Pro Ile Ser Glu Tyr Ser Tyr Ser Ser Thr Ala Thr Asn His             100 105 110 Ser Cys Glu Thr Thr Val Thr Asp Pro Glu Asn Arg Leu Val Leu Gly         115 120 125 Gln Glu Glu Glu Asp Glu Asp Glu Ala Glu Ala Ala Ser Trp Leu Leu     130 135 140 Pro Asn Ser Gly Lys Asn Ser Gly Asn Asn Asn Gly Phe Ser Ile Gly 145 150 155 160 Asp Glu Phe Leu Asn Leu Val Asp Tyr Ser Ser Ser Asp Lys Gln Phe                 165 170 175 Thr Asp Gln Ser Asn Gln Tyr Gln Leu Asp Cys Asn Val Pro Gln Arg             180 185 190 Ser Tyr Gly Glu Asp Gly Val Val Pro Leu Gln Ile Glu Val Ser Lys         195 200 205 Gly Met Tyr Gln Glu Gln Gln Asn Phe Gln Leu Ser Ile Asn Cys Gly     210 215 220 Ser Trp Gly Ala Leu Arg Ser Ser Asn Gly Ser Leu Ser His Met Val 225 230 235 240 Asn Val Ser Ser Met Asp Leu Gly Val Val Pro Glu Ser Thr Thr Ser                 245 250 255 Asp Ala Thr Val Ser Asn Pro Arg Ser Pro Lys Ala Val Thr Asp Gln             260 265 270 Pro Pro Tyr Pro Pro Ala Gln Met Leu Ser Pro Arg Asp Arg Glu Ala         275 280 285 Arg Val Leu Arg Tyr Arg Glu Lys Lys Lys Met Arg Lys Phe Glu Lys     290 295 300 Thr Ile Arg Tyr Ala Ser Arg Lys Ala Tyr Ala Glu Lys Arg Pro Arg 305 310 315 320 Ile Lys Gly Arg Phe Ala Lys Lys Lys Asp Val Asp Glu Glu Ala Asn                 325 330 335 Gln Ala Phe Ser Thr Met Ile Thr Phe Asp Thr Gly Tyr Gly Ile Val             340 345 350 Pro Ser Phe         355 <210> 29 <211> 1116 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (191) .. (934) <400> 29 atcccaccta cttgttcccc acaaaacact ctctccctct ttgttctttc atcttctcta 60 agctctttct ctgaacctac gcttctgcta agctattcta agagaagcca gactagcaat 120 aaacccttca ttttaagcat tctgtttcct tcttgagaaa cctagatatt ttggtttctt 180 gtatccggtg atg aag ata cag tgt gat gtg tgt gag aaa gct ccg gcg 229            Met Lys Ile Gln Cys Asp Val Cys Glu Lys Ala Pro Ala              1 5 10 acg gtg att tgt tgc gcc gac gaa gct gct ctc tgt cct caa tgc gac 277 Thr Val Ile Cys Cys Ala Asp Glu Ala Ala Leu Cys Pro Gln Cys Asp      15 20 25 atc gag att cac gcc gct aac aaa ctc gct agc aag cac caa cgt ctt 325 Ile Glu Ile His Ala Ala Asn Lys Leu Ala Ser Lys His Gln Arg Leu  30 35 40 45 cat ctt aat tcc ctc tcc acc aaa ttc cct cgt tgc gat atc tgc caa 373 His Leu Asn Ser Leu Ser Thr Lys Phe Pro Arg Cys Asp Ile Cys Gln                  50 55 60 gag aag gca gct ttc att ttc tgt gta gag gat aga gct ctg ctt tgc 421 Glu Lys Ala Ala Phe Ile Phe Cys Val Glu Asp Arg Ala Leu Leu Cys              65 70 75 agg gac tgc gat gaa tcc atc cac gtg gct aat tct cga tct gct aat 469 Arg Asp Cys Asp Glu Ser Ile His Val Ala Asn Ser Arg Ser Ala Asn          80 85 90 cac cag agg ttc tta gcc act ggg atc aaa gta gct ctg acc tca act 517 His Gln Arg Phe Leu Ala Thr Gly Ile Lys Val Ala Leu Thr Ser Thr      95 100 105 ata tgt agt aaa gaa att gag aag aat caa cct gag cct tcc aac aac 565 Ile Cys Ser Lys Glu Ile Glu Lys Asn Gln Pro Glu Pro Ser Asn Asn 110 115 120 125 caa cag aag gct aat cag att cct gct aaa tcc aca agc cag cag caa 613 Gln Gln Lys Ala Asn Gln Ile Pro Ala Lys Ser Thr Ser Gln Gln Gln                 130 135 140 caa caa cct tct tct gct act cca ctt ccc tgg gct gtt gac gat ttc 661 Gln Gln Pro Ser Ser Ala Thr Pro Leu Pro Trp Ala Val Asp Asp Phe             145 150 155 ttt cac ttc tct gat att gaa tcc acc gac aag aaa gga cag ctt gat 709 Phe His Phe Ser Asp Ile Glu Ser Thr Asp Lys Lys Gly Gln Leu Asp         160 165 170 ctt ggg gca ggg gag ttg gat tgg ttt tca gac atg gga ttc ttc ggt 757 Leu Gly Ala Gly Glu Leu Asp Trp Phe Ser Asp Met Gly Phe Phe Gly     175 180 185 gat cag att aat gac aag gct ctt cct gca gct gaa gtt cct gag ctt 805 Asp Gln Ile Asn Asp Lys Ala Leu Pro Ala Ala Glu Val Pro Glu Leu 190 195 200 205 tct gtt tcg cat tta ggt cat gtt cat tca tac aaa cct atg aag tca 853 Ser Val Ser His Leu Gly His Val His Ser Tyr Lys Pro Met Lys Ser                 210 215 220 aat gtt tca cac aag aag ccg agg ttt gag acc aga tat gat gat gat 901 Asn Val Ser His Lys Lys Pro Arg Phe Glu Thr Arg Tyr Asp Asp Asp             225 230 235 gat gag gaa cac ttc att gtc cct gat ctt ggc taaaaagcta tatgtaatct 954 Asp Glu Glu His Phe Ile Val Pro Asp Leu Gly         240 245 atgtgtagac attcttcaat gtaaaagaac aaacaagaaa cctatctgca tgtgtggagt 1014 taatgtcata tacattttag ttttgtctta agttgtgtaa gatatgttga gagcttataa 1074 caaatgtctg tgtttgagtt ttgttcaaaa aaaaaaaaaa aa 1116 <210> 30 <211> 248 <212> PRT <213> Arabidopsis thaliana <400> 30 Met Lys Ile Gln Cys Asp Val Cys Glu Lys Ala Pro Ala Thr Val Ile   1 5 10 15 Cys Cys Ala Asp Glu Ala Ala Leu Cys Pro Gln Cys Asp Ile Glu Ile              20 25 30 His Ala Ala Asn Lys Leu Ala Ser Lys His Gln Arg Leu His Leu Asn          35 40 45 Ser Leu Ser Thr Lys Phe Pro Arg Cys Asp Ile Cys Gln Glu Lys Ala      50 55 60 Ala Phe Ile Phe Cys Val Glu Asp Arg Ala Leu Leu Cys Arg Asp Cys  65 70 75 80 Asp Glu Ser Ile His Val Ala Asn Ser Arg Ser Ala Asn His Gln Arg                  85 90 95 Phe Leu Ala Thr Gly Ile Lys Val Ala Leu Thr Ser Thr Ile Cys Ser             100 105 110 Lys Glu Ile Glu Lys Asn Gln Pro Glu Pro Ser Asn Asn Gln Gln Lys         115 120 125 Ala Asn Gln Ile Pro Ala Lys Ser Thr Ser Gln Gln Gln Gln Gln Pro     130 135 140 Ser Ser Ala Thr Pro Leu Pro Trp Ala Val Asp Asp Phe Phe His Phe 145 150 155 160 Ser Asp Ile Glu Ser Thr Asp Lys Lys Gly Gln Leu Asp Leu Gly Ala                 165 170 175 Gly Glu Leu Asp Trp Phe Ser Asp Met Gly Phe Phe Gly Asp Gln Ile             180 185 190 Asn Asp Lys Ala Leu Pro Ala Ala Glu Val Pro Glu Leu Ser Val Ser         195 200 205 His Leu Gly His Val His Ser Tyr Lys Pro Met Lys Ser Asn Val Ser     210 215 220 His Lys Lys Pro Arg Phe Glu Thr Arg Tyr Asp Asp Asp Asp Glu Glu 225 230 235 240 His Phe Ile Val Pro Asp Leu Gly                 245 <210> 31 <211> 909 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (906) <400> 31 atg gat cag tac tca tcc tct ttg gtc gat act tca tta gat ctc act 48 Met Asp Gln Tyr Ser Ser Ser Leu Val Asp Thr Ser Leu Asp Leu Thr   1 5 10 15 att ggc gtt act cgt atg cga gtt gaa gaa gat cca ccg aca agt gct 96 Ile Gly Val Thr Arg Met Arg Val Glu Glu Asp Pro Pro Thr Ser Ala              20 25 30 ttg gtg gaa gaa tta aac cga gtt agt gct gag aac aag aag ctc tcg 144 Leu Val Glu Glu Leu Asn Arg Val Ser Ala Glu Asn Lys Lys Leu Ser          35 40 45 gag atg cta act ttg atg tgt gac aac tac aac gtc ttg agg aag caa 192 Glu Met Leu Thr Leu Met Cys Asp Asn Tyr Asn Val Leu Arg Lys Gln      50 55 60 ctt atg gaa tat gtt aac aag agc aac ata acc gag agg gat caa atc 240 Leu Met Glu Tyr Val Asn Lys Ser Asn Ile Thr Glu Arg Asp Gln Ile  65 70 75 80 agc cct ccc aag aaa cgc aaa tcc ccg gcg aga gag gac gca ttc agc 288 Ser Pro Pro Lys Lys Arg Lys Ser Pro Ala Arg Glu Asp Ala Phe Ser                  85 90 95 tgc gcg gtt att ggc gga gtg tcg gag agt agc tca acg gat caa gat 336 Cys Ala Val Ile Gly Gly Val Ser Glu Ser Ser Ser Thr Asp Gln Asp             100 105 110 gag tat ttg tgt aag aag cag aga gaa gag act gtc gtg aag gag aaa 384 Glu Tyr Leu Cys Lys Lys Gln Arg Glu Glu Thr Val Val Lys Glu Lys         115 120 125 gtc tca agg gtc tat tac aag acc gaa gct tct gac act acc ctc gtt 432 Val Ser Arg Val Tyr Tyr Lys Thr Glu Ala Ser Asp Thr Thr Leu Val     130 135 140 gtg aaa gat ggg tat caa tgg agg aaa tat gga cag aaa gtg act aga 480 Val Lys Asp Gly Tyr Gln Trp Arg Lys Tyr Gly Gln Lys Val Thr Arg 145 150 155 160 gac aat cca tct cca aga gct tac ttc aaa tgt gct tgt gct cca agc 528 Asp Asn Pro Ser Pro Arg Ala Tyr Phe Lys Cys Ala Cys Ala Pro Ser                 165 170 175 tgt tct gtc aaa aag aag gtt cag aga agt gtg gag gat cag tcc gtg 576 Cys Ser Val Lys Lys Lys Val Gln Arg Ser Val Glu Asp Gln Ser Val             180 185 190 tta gtt gca act tat gag ggt gaa cac aac cat cca atg cca tcg cag 624 Leu Val Ala Thr Tyr Glu Gly Glu His Asn His Pro Met Pro Ser Gln         195 200 205 atc gat tca aac aat ggc tta aac cgc cac atc tct cat ggt ggt tca 672 Ile Asp Ser Asn Asn Gly Leu Asn Arg His Ile Ser His Gly Gly Ser     210 215 220 gct tca aca ccc gtt gca gca aac aga aga agt agc ttg act gtg ccg 720 Ala Ser Thr Pro Val Ala Ala Asn Arg Arg Ser Ser Leu Thr Val Pro 225 230 235 240 gtg act acc gta gat atg att gaa tcg aag aaa gtg acg agc cca acg 768 Val Thr Thr Val Asp Met Ile Glu Ser Lys Lys Val Thr Ser Pro Thr                 245 250 255 tca aga atc gat ttt ccc caa gtt cag aaa ctt ttg gtg gag caa atg 816 Ser Arg Ile Asp Phe Pro Gln Val Gln Lys Leu Leu Val Glu Gln Met             260 265 270 gct tct tcc tta acc aaa gat cct aac ttt aca gca gct tta gca gca 864 Ala Ser Ser Leu Thr Lys Asp Pro Asn Phe Thr Ala Ala Leu Ala Ala         275 280 285 gct gtt acc gga aaa ttg tat caa cag aat cat acc gag aaa tag 909 Ala Val Thr Gly Lys Leu Tyr Gln Gln Asn His Thr Glu Lys     290 295 300 <210> 32 <211> 302 <212> PRT <213> Arabidopsis thaliana <400> 32 Met Asp Gln Tyr Ser Ser Ser Leu Val Asp Thr Ser Leu Asp Leu Thr   1 5 10 15 Ile Gly Val Thr Arg Met Arg Val Glu Glu Asp Pro Pro Thr Ser Ala              20 25 30 Leu Val Glu Glu Leu Asn Arg Val Ser Ala Glu Asn Lys Lys Leu Ser          35 40 45 Glu Met Leu Thr Leu Met Cys Asp Asn Tyr Asn Val Leu Arg Lys Gln      50 55 60 Leu Met Glu Tyr Val Asn Lys Ser Asn Ile Thr Glu Arg Asp Gln Ile  65 70 75 80 Ser Pro Pro Lys Lys Arg Lys Ser Pro Ala Arg Glu Asp Ala Phe Ser                  85 90 95 Cys Ala Val Ile Gly Gly Val Ser Glu Ser Ser Ser Thr Asp Gln Asp             100 105 110 Glu Tyr Leu Cys Lys Lys Gln Arg Glu Glu Thr Val Val Lys Glu Lys         115 120 125 Val Ser Arg Val Tyr Tyr Lys Thr Glu Ala Ser Asp Thr Thr Leu Val     130 135 140 Val Lys Asp Gly Tyr Gln Trp Arg Lys Tyr Gly Gln Lys Val Thr Arg 145 150 155 160 Asp Asn Pro Ser Pro Arg Ala Tyr Phe Lys Cys Ala Cys Ala Pro Ser                 165 170 175 Cys Ser Val Lys Lys Lys Val Gln Arg Ser Val Glu Asp Gln Ser Val             180 185 190 Leu Val Ala Thr Tyr Glu Gly Glu His Asn His Pro Met Pro Ser Gln         195 200 205 Ile Asp Ser Asn Asn Gly Leu Asn Arg His Ile Ser His Gly Gly Ser     210 215 220 Ala Ser Thr Pro Val Ala Ala Asn Arg Arg Ser Ser Leu Thr Val Pro 225 230 235 240 Val Thr Thr Val Asp Met Ile Glu Ser Lys Lys Val Thr Ser Pro Thr                 245 250 255 Ser Arg Ile Asp Phe Pro Gln Val Gln Lys Leu Leu Val Glu Gln Met             260 265 270 Ala Ser Ser Leu Thr Lys Asp Pro Asn Phe Thr Ala Ala Leu Ala Ala         275 280 285 Ala Val Thr Gly Lys Leu Tyr Gln Gln Asn His Thr Glu Lys     290 295 300 <210> 33 <211> 1462 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (382) .. (1161) <400> 33 accgaccttc ttggttcttc cggcgttgac tgttacgaag atgatgaaga cttgagagtt 60 tctgggtcga gttttggtgg gtactatcca gagagaaccg ggtctggttt acctaagttc 120 aagacggctc aaccaccacc tcttccgatt tcacaatctt ctcataactt cactttctcc 180 gattaccttg attctcctct gcttctcagc tcctcacaca gtttgatatc tccaacaaca 240 ggaacgtttc cattgcaagg ctttaatgga acaacaaaca atcactcaga ttttccctgg 300 cagctacaat ctcaaccatc aaacgcttct tctgctttgc aagaaacata tggtgttcaa 360 gatcacgaga agaagcagga g atg att cct aat gag att gca aca caa aac 411                         Met Ile Pro Asn Glu Ile Ala Thr Gln Asn                           1 5 10 aac aat caa agt ttt gga aca gaa cgt cag ata aag ata cca gca tac 459 Asn Asn Gln Ser Phe Gly Thr Glu Arg Gln Ile Lys Ile Pro Ala Tyr                  15 20 25 atg gtg agt agg aac tct aat gat ggt tat ggt tgg aga aaa tac ggt 507 Met Val Ser Arg Asn Ser Asn Asp Gly Tyr Gly Trp Arg Lys Tyr Gly              30 35 40 cag aaa caa gtg aag aag agc gaa aac cct agg agt tac ttc aag tgt 555 Gln Lys Gln Val Lys Lys Ser Glu Asn Pro Arg Ser Tyr Phe Lys Cys          45 50 55 acg tat cct gat tgt gtt tcc aag aag att gtt gag acg gct tct gat 603 Thr Tyr Pro Asp Cys Val Ser Lys Lys Ile Val Glu Thr Ala Ser Asp      60 65 70 gga cag atc act gag atc att tat aaa ggt ggt cat aat cat cct aag 651 Gly Gln Ile Thr Glu Ile Ile Tyr Lys Gly Gly His Asn His Pro Lys  75 80 85 90 cct gag ttc acc aag aga cca tct caa tct tca tta cca tca tcg gtt 699 Pro Glu Phe Thr Lys Arg Pro Ser Gln Ser Ser Leu Pro Ser Ser Val                  95 100 105 aat ggg agg cgc ttg ttt aat cct gct tct gtt gtt agt gaa cct cat 747 Asn Gly Arg Arg Leu Phe Asn Pro Ala Ser Val Val Ser Glu Pro His             110 115 120 gat caa tca gag aac tct tcg att tcg ttt gac tat agt gat ctt gag 795 Asp Gln Ser Glu Asn Ser Ser Ile Ser Phe Asp Tyr Ser Asp Leu Glu         125 130 135 cag aaa agt ttt aaa tca gag tat ggt gag ata gat gaa gag gag gaa 843 Gln Lys Ser Phe Lys Ser Glu Tyr Gly Glu Ile Asp Glu Glu Glu Glu     140 145 150 caa cct gag atg aag agg atg aaa aga gaa ggt gaa gat gaa ggg atg 891 Gln Pro Glu Met Lys Arg Met Lys Arg Glu Gly Glu Asp Glu Gly Met 155 160 165 170 tct ata gaa gta agc aaa gga gtt aaa gag cca aga gtt gtg gtt cag 939 Ser Ile Glu Val Ser Lys Gly Val Lys Glu Pro Arg Val Val Val Gln                 175 180 185 aca ata agt gat att gat gtt ctt ata gat ggc ttt aga tgg agg aaa 987 Thr Ile Ser Asp Ile Asp Val Leu Ile Asp Gly Phe Arg Trp Arg Lys             190 195 200 tat ggt caa aaa gtt gtc aaa gga aat act aat cca agg agc tac tac 1035 Tyr Gly Gln Lys Val Val Lys Gly Asn Thr Asn Pro Arg Ser Tyr Tyr         205 210 215 Lys Cys Thr Phe Gln Gly Cys Gly Val Lys Lys Gln Val Glu Arg Ser     220 225 230 gca gca gac gag aga gca gtt ctc act acc tat gaa gga aga cac aat 1131 Ala Ala Asp Glu Arg Ala Val Leu Thr Thr Tyr Glu Gly Arg His Asn 235 240 245 250 cac gat atc cca acc gcg cta cgt cgc tcg tgaaattatt gggacttagt 1181 His Asp Ile Pro Thr Ala Leu Arg Arg Ser                 255 260 cactagtaat atgatttagg ctttctaaaa acaaaaaatc ttactatggc ttatcttttg 1241 tgctcattca cagtttgttt atttgtttgt tacacagtca atactttgtt ttgtacagag 1301 tggtgcttag tagtgttttt attattatct tggccttata gaataacctc tcttctcatc 1361 tgtgtgactt taaacacttg agagtccatt ttatagttct tgtgtattgg tcttttgttt 1421 gatttatgta catttttaat attcgaaaaa aaaaaaaaaa a 1462 <210> 34 <211> 260 <212> PRT <213> Arabidopsis thaliana <400> 34 Met Ile Pro Asn Glu Ile Ala Thr Gln Asn Asn Asn Gln Ser Phe Gly   1 5 10 15 Thr Glu Arg Gln Ile Lys Ile Pro Ala Tyr Met Val Ser Arg Asn Ser              20 25 30 Asn Asp Gly Tyr Gly Trp Arg Lys Tyr Gly Gln Lys Gln Val Lys Lys          35 40 45 Ser Glu Asn Pro Arg Ser Tyr Phe Lys Cys Thr Tyr Pro Asp Cys Val      50 55 60 Ser Lys Lys Ile Val Glu Thr Ala Ser Asp Gly Gln Ile Thr Glu Ile  65 70 75 80 Ile Tyr Lys Gly Gly His Asn His Pro Lys Pro Glu Phe Thr Lys Arg                  85 90 95 Pro Ser Gln Ser Ser Leu Pro Ser Ser Val Asn Gly Arg Arg Leu Phe             100 105 110 Asn Pro Ala Ser Val Val Ser Glu Pro His Asp Gln Ser Glu Asn Ser         115 120 125 Ser Ile Ser Phe Asp Tyr Ser Asp Leu Glu Gln Lys Ser Phe Lys Ser     130 135 140 Glu Tyr Gly Glu Ile Asp Glu Glu Glu Glu Gln Pro Glu Met Lys Arg 145 150 155 160 Met Lys Arg Glu Gly Glu Asp Glu Gly Met Ser Ile Glu Val Ser Lys                 165 170 175 Gly Val Lys Glu Pro Arg Val Val Val Gln Thr Ile Ser Asp Ile Asp             180 185 190 Val Leu Ile Asp Gly Phe Arg Trp Arg Lys Tyr Gly Gln Lys Val Val         195 200 205 Lys Gly Asn Thr Asn Pro Arg Ser Tyr Tyr Lys Cys Thr Phe Gln Gly     210 215 220 Cys Gly Val Lys Lys Gln Val Glu Arg Ser Ala Ala Asp Glu Arg Ala 225 230 235 240 Val Leu Thr Thr Tyr Glu Gly Arg His Asn His Asp Ile Pro Thr Ala                 245 250 255 Leu Arg Arg Ser             260 <210> 35 <211> 438 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (435) <400> 35 atg gag gga tat gat aat ggg tcg ttg tat gct cct ttt ttg tcg ttg 48 Met Glu Gly Tyr Asp Asn Gly Ser Leu Tyr Ala Pro Phe Leu Ser Leu   1 5 10 15 aaa tct cat tcg aaa cca gag ctg cat caa ggc gaa gaa gag agc tca 96 Lys Ser His Ser Lys Pro Glu Leu His Gln Gly Glu Glu Glu Ser Ser              20 25 30 aag gtt aga tca gaa ggt tgt tcg aaa agc gtg gag tcg tcg aaa aag 144 Lys Val Arg Ser Glu Gly Cys Ser Lys Ser Val Glu Ser Ser Lys Lys          35 40 45 aag ggg aag aaa caa agg tat gcg ttt caa aca agg agc caa gtg gat 192 Lys Gly Lys Lys Gln Arg Tyr Ala Phe Gln Thr Arg Ser Gln Val Asp      50 55 60 att ctt gat gat ggt tat cga tgg agg aaa tat ggc caa aag gcc gtc 240 Ile Leu Asp Asp Gly Tyr Arg Trp Arg Lys Tyr Gly Gln Lys Ala Val  65 70 75 80 aag aac aac aag ttc cct agg agt tac tat agg tgt aca tat gga gga 288 Lys Asn Asn Lys Phe Pro Arg Ser Tyr Tyr Arg Cys Thr Tyr Gly Gly                  85 90 95 tgc aat gtg aag aag caa gtg caa aga tta aca gtg gac caa gaa gtg 336 Cys Asn Val Lys Lys Gln Val Gln Arg Leu Thr Val Asp Gln Glu Val             100 105 110 gtc gtg aca acc tac gaa gga gtg cat tcg cat ccc atc gag aaa tcc 384 Val Val Thr Thr Tyr Glu Gly Val His Ser His Pro Ile Glu Lys Ser         115 120 125 acc gaa aac ttc gag cat att ctc act caa atg caa atc tac tct tct 432 Thr Glu Asn Phe Glu His Ile Leu Thr Gln Met Gln Ile Tyr Ser Ser     130 135 140 ttc tag 438 Phe 145 <210> 36 <211> 145 <212> PRT <213> Arabidopsis thaliana <400> 36 Met Glu Gly Tyr Asp Asn Gly Ser Leu Tyr Ala Pro Phe Leu Ser Leu   1 5 10 15 Lys Ser His Ser Lys Pro Glu Leu His Gln Gly Glu Glu Glu Ser Ser              20 25 30 Lys Val Arg Ser Glu Gly Cys Ser Lys Ser Val Glu Ser Ser Lys Lys          35 40 45 Lys Gly Lys Lys Gln Arg Tyr Ala Phe Gln Thr Arg Ser Gln Val Asp      50 55 60 Ile Leu Asp Asp Gly Tyr Arg Trp Arg Lys Tyr Gly Gln Lys Ala Val  65 70 75 80 Lys Asn Asn Lys Phe Pro Arg Ser Tyr Tyr Arg Cys Thr Tyr Gly Gly                  85 90 95 Cys Asn Val Lys Lys Gln Val Gln Arg Leu Thr Val Asp Gln Glu Val             100 105 110 Val Val Thr Thr Tyr Glu Gly Val His Ser His Pro Ile Glu Lys Ser         115 120 125 Thr Glu Asn Phe Glu His Ile Leu Thr Gln Met Gln Ile Tyr Ser Ser     130 135 140 Phe 145 <210> 37 <211> 1254 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (137) .. (1090) <400> 37 aaaagtccaa gcaccaatct agacctctta ggaaaaaaac ctaaaaacct aatccccaaa 60 cctaaaaggc ttatctcatc tcttcttctt tgtcttcttt actctttttt tacctctctc 120 ttcattgttc ttcacc atg tct aat gaa acc aga gat ctc tac aac tac caa 172                   Met Ser Asn Glu Thr Arg Asp Leu Tyr Asn Tyr Gln                     1 5 10 tac cct tca tcg ttt tcg ttg cac gaa atg atg aat ctg cct act tca 220 Tyr Pro Ser Ser Phe Ser Leu His Glu Met Met Asn Leu Pro Thr Ser          15 20 25 aat cca tct tct tat gga aac ctc cca tca caa aac ggt ttt aat cca 268 Asn Pro Ser Ser Tyr Gly Asn Leu Pro Ser Gln Asn Gly Phe Asn Pro      30 35 40 tct act tat tcc ttc acc gat tgt ctc caa agt tct cca gca gcg tat 316 Ser Thr Tyr Ser Phe Thr Asp Cys Leu Gln Ser Ser Pro Ala Ala Tyr  45 50 55 60 gaa tct cta ctt cag aaa act ttt ggt ctt tct ccc tct tcc tca gag 364 Glu Ser Leu Leu Gln Lys Thr Phe Gly Leu Ser Pro Ser Ser Ser Glu                  65 70 75 gtt ttc aat tct tcg atc gat caa gaa ccg aac cgt gat gtt act aat 412 Val Phe Asn Ser Ser Ile Asp Gln Glu Pro Asn Arg Asp Val Thr Asn              80 85 90 gac gta atc aat ggt ggt gca tgc aac gag act gaa act agg gtt tct 460 Asp Val Ile Asn Gly Gly Ala Cys Asn Glu Thr Glu Thr Arg Val Ser          95 100 105 cct tct aat tct tcc tct agt gag gct gat cac ccc ggt gaa gat tcc 508 Pro Ser Asn Ser Ser Ser Ser Glu Ala Asp His Pro Gly Glu Asp Ser     110 115 120 ggt aag agc cgg agg aaa cga gag tta gtc ggt gaa gaa gat caa att 556 Gly Lys Ser Arg Arg Lys Arg Glu Leu Val Gly Glu Glu Asp Gln Ile 125 130 135 140 tcc aaa aaa gtt ggg aaa acg aaa aag act gag gtg aag aaa caa aga 604 Ser Lys Lys Val Gly Lys Thr Lys Lys Thr Glu Val Lys Lys Gln Arg                 145 150 155 gag cca cga gtc tcg ttt atg act aaa agt gaa gtt gat cat ctt gaa 652 Glu Pro Arg Val Ser Phe Met Thr Lys Ser Glu Val Asp His Leu Glu             160 165 170 gat ggt tat aga tgg aga aaa tac ggc caa aag gct gta aaa aat agc 700 Asp Gly Tyr Arg Trp Arg Lys Tyr Gly Gln Lys Ala Val Lys Asn Ser         175 180 185 cct tat cca agg agt tac tat aga tgt aca aca caa aag tgc aac gtg 748 Pro Tyr Pro Arg Ser Tyr Tyr Arg Cys Thr Thr Gln Lys Cys Asn Val     190 195 200 aag aaa cga gtg gag aga tcg ttc caa gat cca acg gtt gtg att aca 796 Lys Lys Arg Val Glu Arg Ser Phe Gln Asp Pro Thr Val Val Ile Thr 205 210 215 220 act tac gag ggt caa cac aac cac ccg att ccg act aat ctt cga gga 844 Thr Tyr Glu Gly Gln His Asn His Pro Ile Pro Thr Asn Leu Arg Gly                 225 230 235 agt tct gcc gcg gct gct atg ttc tcc gca gac ctc atg act cca aga 892 Ser Ser Ala Ala Ala Ala Met Phe Ser Ala Asp Leu Met Thr Pro Arg             240 245 250 agc ttt gca cat gat atg ttt agg acg gca gct tat act aac ggc ggt 940 Ser Phe Ala His Asp Met Phe Arg Thr Ala Ala Tyr Thr Asn Gly Gly         255 260 265 tct gtg gcg gcg gct ttg gat tat gga tat gga caa agt ggt tat ggt 988 Ser Val Ala Ala Ala Leu Asp Tyr Gly Tyr Gly Gln Ser Gly Tyr Gly     270 275 280 agt gtg aat tca aac cct agt tct cac caa gtg tat cat caa ggg ggt 1036 Ser Val Asn Ser Asn Pro Ser Ser His Gln Val Tyr His Gln Gly Gly 285 290 295 300 Glu Tyr Glu Leu Leu Arg Glu Ile Phe Pro Ser Ile Phe Phe Lys Gln                 305 310 315 gag cct tgatcgatca ttgttataac tacatatatt atatatattg agagagagag 1140 Glu Pro gtagagaaaa aaaaaactta tatgtaactt aagatcttat tttgtctctc ttatttgcat 1200 gtacatattt tttcatgaaa gaatgagaca gttgggcttg cttaaaaaaa aaat 1254 <210> 38 <211> 318 <212> PRT <213> Arabidopsis thaliana <400> 38 Met Ser Asn Glu Thr Arg Asp Leu Tyr Asn Tyr Gln Tyr Pro Ser Ser   1 5 10 15 Phe Ser Leu His Glu Met Met Asn Leu Pro Thr Ser Asn Pro Ser Ser              20 25 30 Tyr Gly Asn Leu Pro Ser Gln Asn Gly Phe Asn Pro Ser Thr Tyr Ser          35 40 45 Phe Thr Asp Cys Leu Gln Ser Ser Pro Ala Ala Tyr Glu Ser Leu Leu      50 55 60 Gln Lys Thr Phe Gly Leu Ser Pro Ser Ser Ser Glu Val Phe Asn Ser  65 70 75 80 Ser Ile Asp Gln Glu Pro Asn Arg Asp Val Thr Asn Asp Val Ile Asn                  85 90 95 Gly Gly Ala Cys Asn Glu Thr Glu Thr Arg Val Ser Pro Ser Asn Ser             100 105 110 Ser Ser Ser Glu Ala Asp His Pro Gly Glu Asp Ser Gly Lys Ser Arg         115 120 125 Arg Lys Arg Glu Leu Val Gly Glu Glu Asp Gln Ile Ser Lys Lys Val     130 135 140 Gly Lys Thr Lys Lys Thr Glu Val Lys Lys Gln Arg Glu Pro Arg Val 145 150 155 160 Ser Phe Met Thr Lys Ser Glu Val Asp His Leu Glu Asp Gly Tyr Arg                 165 170 175 Trp Arg Lys Tyr Gly Gln Lys Ala Val Lys Asn Ser Pro Tyr Pro Arg             180 185 190 Ser Tyr Tyr Arg Cys Thr Thr Gln Lys Cys Asn Val Lys Lys Arg Val         195 200 205 Glu Arg Ser Phe Gln Asp Pro Thr Val Val Ile Thr Thr Tyr Glu Gly     210 215 220 Gln His Asn His Pro Ile Pro Thr Asn Leu Arg Gly Ser Ser Ala Ala 225 230 235 240 Ala Ala Met Phe Ser Ala Asp Leu Met Thr Pro Arg Ser Phe Ala His                 245 250 255 Asp Met Phe Arg Thr Ala Ala Tla Thr Asn Gly Gly Ser Val Ala Ala             260 265 270 Ala Leu Asp Tyr Gly Tyr Gly Gln Ser Gly Tyr Gly Ser Val Asn Ser         275 280 285 Asn Pro Ser Ser His Gln Val Tyr His Gln Gly Gly Glu Tyr Glu Leu     290 295 300 Leu Arg Glu Ile Phe Pro Ser Ile Phe Phe Lys Gln Glu Pro 305 310 315 <210> 39 <211> 1148 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (104) .. (1075) <400> 39 acgtctctct ctttctctct actctctgtt tcctcataat tcaatcacta tattttttta 60 aaaacatttg acttcatcga tcggttaaca attaatcaaa aag atg gga cga tca 115                                                 Met Gly Arg Ser                                                   1 cca tgt tgt gag aag aag aat ggt ctc aag aaa gga cca tgg act cct 163 Pro Cys Cys Glu Lys Lys Asn Gly Leu Lys Lys Gly Pro Trp Thr Pro   5 10 15 20 gag gag gat caa aag ctc att gat tat atc aat ata cat ggt tat gga 211 Glu Glu Asp Gln Lys Leu Ile Asp Tyr Ile Asn Ile His Gly Tyr Gly                  25 30 35 aat tgg aga act ctt ccc aag aat gct ggg tta caa aga tgt ggt aag 259 Asn Trp Arg Thr Leu Pro Lys Asn Ala Gly Leu Gln Arg Cys Gly Lys              40 45 50 agt tgt cgt ctc cgg tgg acc aac tat ctc cga cca gat att aag cgt 307 Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro Asp Ile Lys Arg          55 60 65 gga aga ttc tct ttt gaa gaa gaa gaa acc att att caa ctt cac agc 355 Gly Arg Phe Ser Phe Glu Glu Glu Glu Thr Ile Ile Gln Leu His Ser      70 75 80 atc atg gga aac aag tgg tct gcg att gcg gct cgt ttg cct gga aga 403 Ile Met Gly Asn Lys Trp Ser Ala Ile Ala Ala Arg Leu Pro Gly Arg  85 90 95 100 aca gac aac gag atc aaa aac tat tgg aac act cac atc aga aaa aga 451 Thr Asp Asn Glu Ile Lys Asn Tyr Trp Asn Thr His Ile Arg Lys Arg                 105 110 115 ctt cta aag atg gga atc gac ccg gtt aca cac act cca cgt ctt gat 499 Leu Leu Lys Met Gly Ile Asp Pro Val Thr His Thr Pro Arg Leu Asp             120 125 130 ctt ctc gat atc tcc tcc att ctc agc tca tct atc tac aac tct tcg 547 Leu Leu Asp Ile Ser Ser Ile Leu Ser Ser Ser Ile Tyr Asn Ser Ser         135 140 145 cat cat cat cat cat cat cat caa caa cat atg aac atg tcg agg ctc 595 His His His His His His Gln Gln His Met Asn Met Ser Arg Leu     150 155 160 atg atg agt gat ggt aat cat caa cca ttg gtt aac ccc gag ata ctc 643 Met Met Ser Asp Gly Asn His Gln Pro Leu Val Asn Pro Glu Ile Leu 165 170 175 180 aaa ctc gca acc tct ctc ttt tca aac caa aac cac ccc aac aac aca 691 Lys Leu Ala Thr Ser Leu Phe Ser Asn Gln Asn His Pro Asn Asn Thr                 185 190 195 cac gag aac aac acg gtt aac caa acc gaa gta aac caa tac caa acc 739 His Glu Asn Asn Thr Val Asn Gln Thr Glu Val Asn Gln Tyr Gln Thr             200 205 210 ggt tac aac atg cct ggt aat gaa gaa tta caa tct tgg ttc cct atc 787 Gly Tyr Asn Met Pro Gly Asn Glu Glu Leu Gln Ser Trp Phe Pro Ile         215 220 225 atg gat caa ttc acg aat ttc caa gac ctc atg cca atg aag acg acg 835 Met Asp Gln Phe Thr Asn Phe Gln Asp Leu Met Pro Met Lys Thr Thr     230 235 240 gtc caa aat tca ttg tca tac gat gat gat tgt tcg aag tcc aat ttt 883 Val Gln Asn Ser Leu Ser Tyr Asp Asp Asp Cys Ser Lys Ser Asn Phe 245 250 255 260 gta tta gaa cct tat tac tcc gac ttt gct tca gtc ttg acc aca cct 931 Val Leu Glu Pro Tyr Tyr Ser Asp Phe Ala Ser Val Leu Thr Thr Pro                 265 270 275 tct tca agc ccg act ccg tta aac tca agt tcc tca act tac atc aat 979 Ser Ser Ser Pro Thr Pro Leu Asn Ser Ser Ser Ser Thr Tyr Ile Asn             280 285 290 agt agc act tgc agc acc gag gat gaa aaa gag agt tat tac agt gat 1027 Ser Ser Thr Cys Ser Thr Glu Asp Glu Lys Glu Ser Tyr Tyr Ser Asp         295 300 305 aat atc act aat tat tcg ttt gat gtt aat ggt ttt ctc caa ttc caa 1075 Asn Ile Thr Asn Tyr Ser Phe Asp Val Asn Gly Phe Leu Gln Phe Gln     310 315 320 taaacaaaac gccattggaa tagagttatg taaacatgca atcattgtat ttgttatata 1135 gattttgtta cat 1148 <210> 40 <211> 324 <212> PRT <213> Arabidopsis thaliana <400> 40 Met Gly Arg Ser Pro Cys Cys Glu Lys Lys Asn Gly Leu Lys Lys Gly   1 5 10 15 Pro Trp Thr Pro Glu Glu Asp Gln Lys Leu Ile Asp Tyr Ile Asn Ile              20 25 30 His Gly Tyr Gly Asn Trp Arg Thr Leu Pro Lys Asn Ala Gly Leu Gln          35 40 45 Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Thr Asn Tyr Leu Arg Pro      50 55 60 Asp Ile Lys Arg Gly Arg Phe Ser Phe Glu Glu Glu Glu Thr Ile Ile  65 70 75 80 Gln Leu His Ser Ile Met Gly Asn Lys Trp Ser Ala Ile Ala Ala Arg                  85 90 95 Leu Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn Tyr Trp Asn Thr His             100 105 110 Ile Arg Lys Arg Leu Leu Lys Met Gly Ile Asp Pro Val Thr His Thr         115 120 125 Pro Arg Leu Asp Leu Leu Asp Ile Ser Ser Ile Leu Ser Ser Ser Ile     130 135 140 Tyr Asn Ser Ser His His His His His His Gln Gln His Met Asn 145 150 155 160 Met Ser Arg Leu Met Met Ser Asp Gly Asn His Gln Pro Leu Val Asn                 165 170 175 Pro Glu Ile Leu Lys Leu Ala Thr Ser Leu Phe Ser Asn Gln Asn His             180 185 190 Pro Asn Asn Thr His Glu Asn Asn Thr Val Asn Gln Thr Glu Val Asn         195 200 205 Gln Tyr Gln Thr Gly Tyr Asn Met Pro Gly Asn Glu Glu Leu Gln Ser     210 215 220 Trp Phe Pro Ile Met Asp Gln Phe Thr Asn Phe Gln Asp Leu Met Pro 225 230 235 240 Met Lys Thr Thr Val Gln Asn Ser Leu Ser Tyr Asp Asp Asp Cys Ser                 245 250 255 Lys Ser Asn Phe Val Leu Glu Pro Tyr Tyr Ser Asp Phe Ala Ser Val             260 265 270 Leu Thr Thr Pro Ser Ser Ser Pro Pro Thr Pro Leu Asn Ser Ser Ser Ser         275 280 285 Thr Tyr Ile Asn Ser Ser Thr Cys Ser Thr Glu Asp Glu Lys Glu Ser     290 295 300 Tyr Tyr Ser Asp Asn Ile Thr Asn Tyr Ser Phe Asp Val Asn Gly Phe 305 310 315 320 Leu Gln Phe Gln <210> 41 <211> 2684 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (898) .. (2475) <400> 41 acttcttctt cttcttcttc tcgatttctt actgttttct tatccaacga aatctggaat 60 taaaaatgga atctttatcg aatccaagct gattttgttt ctttcattga atcatctctc 120 taaagtggaa ttttgtaaag agaagatctg aagttgtgta gaggagctta gtgatggaga 180 caaattcgtc tggagaagat ctggttatta agactcggaa gccatatacg ataacaaagc 240 aacgtgaaag gtggactgag gaagaacata atagattcat tgaagctttg aggctttatg 300 gtagagcatg gcagaagatt gaagaacatg tagcaacaaa aactgctgtc cagataagaa 360 gtcacgctca gaaatttttc tccaaggtaa aatcggttaa ttttgaaatg atgttctcat 420 cttcattggc ttaatgctta agacttattg aaagccaggc aagttttctg cttcttttgc 480 ttcttagtca ggagatagat agattacgtt tttagagttt agtaatgagc aataagtctt 540 aaaatagttg gagaaatgac gagatgtaat cgttttcttt tgtttatgcc tatatcttgt 600 taatccacaa acatgtacat agattcttca gaagaatgtt agtttcttta gattcttcag 660 ataaacttgt gtcttcttac cgattctgag gtagtggcaa aagtgggctg agtgctagaa 720 atttttgaat gttccttgtg ataagccata gaggtaaacc atttttgatt ttccagttct 780 gtcatttaaa cttgttagtg tcattagatt tttgtttgtt tacgtttgtt tagagggtaa 840 caaaactact ctcatctctc tcaggtagag aaagaggctg aagctaaagg tgtagct 897 atg ggt caa gcg cta gac ata gct att cct cct cca cgg cct aag cgt 945 Met Gly Gln Ala Leu Asp Ile Ala Ile Pro Pro Pro Arg Pro Lys Arg   1 5 10 15 aaa cca aac aat cct tat cct cga aag acg gga agt gga acg atc ctt 993 Lys Pro Asn Asn Pro Tyr Pro Arg Lys Thr Gly Ser Gly Thr Ile Leu              20 25 30 atg tca aaa acg ggt gtg aat gat gga aaa gag tcc ctt gga tca gaa 1041 Met Ser Lys Thr Gly Val Asn Asp Gly Lys Glu Ser Leu Gly Ser Glu          35 40 45 aaa gtg tcg cat cct gag atg gcc aat gaa gat cga caa caa tca aag 1089 Lys Val Ser His Pro Glu Met Ala Asn Glu Asp Arg Gln Gln Ser Lys      50 55 60 cct gaa gag aaa act ctg cag gaa gac aac tgt tca gat tgt ttc act 1137 Pro Glu Glu Lys Thr Leu Gln Glu Asp Asn Cys Ser Asp Cys Phe Thr  65 70 75 80 cat cag tat ctc tct gct gca tcc tcc atg aat aaa agt tgt ata gag 1185 His Gln Tyr Leu Ser Ala Ala Ser Ser Met Asn Lys Ser Cys Ile Glu                  85 90 95 aca tca aac gca agc act ttc cgc gag ttc ttg cct tca cgg gaa gag 1233 Thr Ser Asn Ala Ser Thr Phe Arg Glu Phe Leu Pro Ser Arg Glu Glu             100 105 110 gga agt cag aat aac agg gta aga aag gag tca aac tca gat ttg aat 1281 Gly Ser Gln Asn Asn Arg Val Arg Lys Glu Ser Asn Ser Asp Leu Asn         115 120 125 gca aaa tct ctg gaa aac ggt aat gag caa gga cct cag act tat ccg 1329 Ala Lys Ser Leu Glu Asn Gly Asn Glu Gln Gly Pro Gln Thr Tyr Pro     130 135 140 atg cat atc cct gtg cta gtg cca ttg ggg agc tca ata aca agt tct 1377 Met His Ile Pro Val Leu Val Pro Leu Gly Ser Ser Ile Thr Ser Ser 145 150 155 160 cta tca cat cct cct tca gag cca gat agt cat ccc cac aca gtt gca 1425 Leu Ser His Pro Pro Ser Glu Pro Asp Ser His Pro His Thr Val Ala                 165 170 175 gga gat tat cag tcg ttt cct aat cat ata atg tca acc ctt tta caa 1473 Gly Asp Tyr Gln Ser Phe Pro Asn His Ile Met Ser Thr Leu Leu Gln             180 185 190 aca ccg gct ctt tat act gcc gca act ttc gcc tca tca ttt tgg cct 1521 Thr Pro Ala Leu Tyr Thr Ala Ala Thr Phe Ala Ser Ser Phe Trp Pro         195 200 205 ccc gat tct agt ggt ggc tca cct gtt cca ggg aac tca cct ccg aat 1569 Pro Asp Ser Ser Gly Gly Ser Pro Val Pro Gly Asn Ser Pro Pro Asn     210 215 220 Leu Ala Ala Met Ala Ala Ala Thr Val Ala Ala Ala Ser Ala Trp Trp 225 230 235 240 gct gcc aat gga tta tta cct tta tgt gct cct ctt agt tca ggt ggt 1665 Ala Ala Asn Gly Leu Leu Pro Leu Cys Ala Pro Leu Ser Ser Gly Gly                 245 250 255 ttc act agt cat cct cca tct act ttt gga cca tca tgt gat gta gag 1713 Phe Thr Ser His Pro Pro Ser Thr Phe Gly Pro Ser Cys Asp Val Glu             260 265 270 tac aca aaa gca agc act tta caa cat ggt tct gtg cag agc cga gag 1761 Tyr Thr Lys Ala Ser Thr Leu Gln His Gly Ser Val Gln Ser Arg Glu         275 280 285 caa gaa cac ttc gag gca tca aag gct cga tct tca ctg gac tca gag 1809 Gln Glu His Phe Glu Ala Ser Lys Ala Arg Ser Ser Leu Asp Ser Glu     290 295 300 gat gtt gaa aat aag agt aaa cca gtt tgt cat gag cag cct tct gca 1857 Asp Val Glu Asn Lys Ser Lys Pro Val Cys His Glu Gln Pro Ser Ala 305 310 315 320 aca cct gag agt gat gca aag ggt tca gat gga gca gga gac aga aaa 1905 Thr Pro Glu Ser Asp Ala Lys Gly Ser Asp Gly Ala Gly Asp Arg Lys                 325 330 335 caa gtt gac cgg tcc tcg tgt ggc tca aac act ccg tcg agt agt gat 1953 Gln Val Asp Arg Ser Ser Cys Gly Ser Asn Thr Pro Ser Ser Ser Asp             340 345 350 gat gtt gag gcg gat gca tca gaa agg caa gag gat ggc acc aat ggt 2001 Asp Val Glu Ala Asp Ala Ser Glu Arg Gln Glu Asp Gly Thr Asn Gly         355 360 365 gag gtg aaa gaa acg aat gaa gac act aat aaa cct caa act tca gag 2049 Glu Val Lys Glu Thr Asn Glu Asp Thr Asn Lys Pro Gln Thr Ser Glu     370 375 380 tcc aat gca cgc cgc agt aga atc agc tcc aat ata acc gat cca tgg 2097 Ser Asn Ala Arg Arg Ser Arg Ile Ser Ser Asn Ile Thr Asp Pro Trp 385 390 395 400 aag tct gtg tct gac gag ggt cga att gcc ttc caa gct ctc ttc tcc 2145 Lys Ser Val Ser Asp Glu Gly Arg Ile Ala Phe Gln Ala Leu Phe Ser                 405 410 415 aga gag gta ttg ccg caa agt ttt aca tat cga gaa gaa cac aga gag 2193 Arg Glu Val Leu Pro Gln Ser Phe Thr Tyr Arg Glu Glu His Arg Glu             420 425 430 gaa gaa caa caa caa caa gaa caa aga tat cca atg gca ctt gat ctt 2241 Glu Glu Gln Gln Gln Gln Glu Gln Arg Tyr Pro Met Ala Leu Asp Leu         435 440 445 aac ttc aca gct cag tta aca cca gtt gat gat caa gag gag aag aga 2289 Asn Phe Thr Ala Gln Leu Thr Pro Val Asp Asp Gln Glu Glu Lys Arg     450 455 460 aac aca gga ttt ctt gga atc gga tta gat gct tca aag cta atg agt 2337 Asn Thr Gly Phe Leu Gly Ile Gly Leu Asp Ala Ser Lys Leu Met Ser 465 470 475 480 aga gga aga aca ggt ttt aaa cca tac aaa aga tgt tcc atg gaa gcc 2385 Arg Gly Arg Thr Gly Phe Lys Pro Tyr Lys Arg Cys Ser Met Glu Ala                 485 490 495 aaa gaa agt aga atc ctc aac aac aat cct atc att cat gtg gaa cag 2433 Lys Glu Ser Arg Ile Leu Asn Asn Asn Pro Ile Ile His Val Glu Gln             500 505 510 aaa gat ccc aaa cgg atg cgg ttg gaa act caa gct tcc aca 2475 Lys Asp Pro Lys Arg Met Arg Leu Glu Thr Gln Ala Ser Thr         515 520 525 tgagactcta ttttcatctg atctgttgtt tgtactctgt ttttaagttt tcaagaccac 2535 tgctacattt tctttttctt ttgaggcctt tgtatttgtt tccttgtcca tagtcttcct 2595 gtaacatttg actctgtatt attcaacaaa tcataaactg tttaatcttt ttttttccag 2655 aaaaaaaaaa aagaaaaaaa aaaaaaaaa 2684 <210> 42 <211> 526 <212> PRT <213> Arabidopsis thaliana <400> 42 Met Gly Gln Ala Leu Asp Ile Ala Ile Pro Pro Pro Arg Pro Lys Arg   1 5 10 15 Lys Pro Asn Asn Pro Tyr Pro Arg Lys Thr Gly Ser Gly Thr Ile Leu              20 25 30 Met Ser Lys Thr Gly Val Asn Asp Gly Lys Glu Ser Leu Gly Ser Glu          35 40 45 Lys Val Ser His Pro Glu Met Ala Asn Glu Asp Arg Gln Gln Ser Lys      50 55 60 Pro Glu Glu Lys Thr Leu Gln Glu Asp Asn Cys Ser Asp Cys Phe Thr  65 70 75 80 His Gln Tyr Leu Ser Ala Ala Ser Ser Met Asn Lys Ser Cys Ile Glu                  85 90 95 Thr Ser Asn Ala Ser Thr Phe Arg Glu Phe Leu Pro Ser Arg Glu Glu             100 105 110 Gly Ser Gln Asn Asn Arg Val Arg Lys Glu Ser Asn Ser Asp Leu Asn         115 120 125 Ala Lys Ser Leu Glu Asn Gly Asn Glu Gln Gly Pro Gln Thr Tyr Pro     130 135 140 Met His Ile Pro Val Leu Val Pro Leu Gly Ser Ser Ile Thr Ser Ser 145 150 155 160 Leu Ser His Pro Pro Ser Glu Pro Asp Ser His Pro His Thr Val Ala                 165 170 175 Gly Asp Tyr Gln Ser Phe Pro Asn His Ile Met Ser Thr Leu Leu Gln             180 185 190 Thr Pro Ala Leu Tyr Thr Ala Ala Thr Phe Ala Ser Ser Phe Trp Pro         195 200 205 Pro Asp Ser Ser Gly Gly Ser Pro Val Pro Gly Asn Ser Pro Pro Asn     210 215 220 Leu Ala Ala Met Ala Ala Ala Thr Val Ala Ala Ala Ser Ala Trp Trp 225 230 235 240 Ala Ala Asn Gly Leu Leu Pro Leu Cys Ala Pro Leu Ser Ser Gly Gly                 245 250 255 Phe Thr Ser His Pro Pro Ser Thr Phe Gly Pro Ser Cys Asp Val Glu             260 265 270 Tyr Thr Lys Ala Ser Thr Leu Gln His Gly Ser Val Gln Ser Arg Glu         275 280 285 Gln Glu His Phe Glu Ala Ser Lys Ala Arg Ser Ser Leu Asp Ser Glu     290 295 300 Asp Val Glu Asn Lys Ser Lys Pro Val Cys His Glu Gln Pro Ser Ala 305 310 315 320 Thr Pro Glu Ser Asp Ala Lys Gly Ser Asp Gly Ala Gly Asp Arg Lys                 325 330 335 Gln Val Asp Arg Ser Ser Cys Gly Ser Asn Thr Pro Ser Ser Ser Asp             340 345 350 Asp Val Glu Ala Asp Ala Ser Glu Arg Gln Glu Asp Gly Thr Asn Gly         355 360 365 Glu Val Lys Glu Thr Asn Glu Asp Thr Asn Lys Pro Gln Thr Ser Glu     370 375 380 Ser Asn Ala Arg Arg Ser Arg Ile Ser Ser Asn Ile Thr Asp Pro Trp 385 390 395 400 Lys Ser Val Ser Asp Glu Gly Arg Ile Ala Phe Gln Ala Leu Phe Ser                 405 410 415 Arg Glu Val Leu Pro Gln Ser Phe Thr Tyr Arg Glu Glu His Arg Glu             420 425 430 Glu Glu Gln Gln Gln Gln Glu Gln Arg Tyr Pro Met Ala Leu Asp Leu         435 440 445 Asn Phe Thr Ala Gln Leu Thr Pro Val Asp Asp Gln Glu Glu Lys Arg     450 455 460 Asn Thr Gly Phe Leu Gly Ile Gly Leu Asp Ala Ser Lys Leu Met Ser 465 470 475 480 Arg Gly Arg Thr Gly Phe Lys Pro Tyr Lys Arg Cys Ser Met Glu Ala                 485 490 495 Lys Glu Ser Arg Ile Leu Asn Asn Asn Pro Ile Ile His Val Glu Gln             500 505 510 Lys Asp Pro Lys Arg Met Arg Leu Glu Thr Gln Ala Ser Thr         515 520 525 <210> 43 <211> 1938 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (1935) <400> 43 atg gat act aat aca tct gga gaa gaa tta tta gct aag gca aga aag 48 Met Asp Thr Asn Thr Ser Gly Glu Glu Leu Leu Ala Lys Ala Arg Lys   1 5 10 15 cca tat aca ata aca aag cag cga gag cga tgg act gag gat gag cat 96 Pro Tyr Thr Ile Thr Lys Gln Arg Glu Arg Trp Thr Glu Asp Glu His              20 25 30 gag agg ttt cta gaa gcc ttg agg ctt tat gga aga gct tgg caa cga 144 Glu Arg Phe Leu Glu Ala Leu Arg Leu Tyr Gly Arg Ala Trp Gln Arg          35 40 45 att gaa gaa cat att ggg aca aag act gct gtt cag atc aga agt cat 192 Ile Glu Glu His Ile Gly Thr Lys Thr Ala Val Gln Ile Arg Ser His      50 55 60 gca caa aag ttc ttc aca aag ttg gag aaa gag gct gaa gtt aaa ggc 240 Ala Gln Lys Phe Phe Thr Lys Leu Glu Lys Glu Ala Glu Val Lys Gly  65 70 75 80 atc cct gtt tgc caa gct ttg gac ata gaa att ccg cct cct cgt cct 288 Ile Pro Val Cys Gln Ala Leu Asp Ile Glu Ile Pro Pro Pro Arg Pro                  85 90 95 aaa cga aaa ccc aat act cct tat cct cga aag cct ggg aac aac ggt 336 Lys Arg Lys Pro Asn Thr Pro Tyr Pro Arg Lys Pro Gly Asn Asn Gly             100 105 110 aca tct tcc tct caa gta tca tca gca aaa gat gca aaa ctt gtt tca 384 Thr Ser Ser Ser Gln Val Ser Ser Ala Lys Asp Ala Lys Leu Val Ser         115 120 125 tcg gcc tct tct tca cag ttg aat cag gcg ttc ttg gat ttg gaa aaa 432 Ser Ala Ser Ser Ser Gln Leu Asn Gln Ala Phe Leu Asp Leu Glu Lys     130 135 140 atg ccg ttc tct gag aaa aca tca act gga aaa gaa aat caa gat gag 480 Met Pro Phe Ser Glu Lys Thr Ser Thr Gly Lys Glu Asn Gln Asp Glu 145 150 155 160 aat tgc tcg ggt gtt tct act gtg aac aag tat ccc tta cca acg aaa 528 Asn Cys Ser Gly Val Ser Thr Val Asn Lys Tyr Pro Leu Pro Thr Lys                 165 170 175 cag gta agt ggc gac att gaa aca agt aag acc tca act gtg gac aac 576 Gln Val Ser Gly Asp Ile Glu Thr Ser Lys Thr Ser Thr Val Asp Asn             180 185 190 gcg gtt caa gat gtt ccc aag aag aac aaa gac aaa gat ggt aac gat 624 Ala Val Gln Asp Val Pro Lys Lys Asn Lys Asp Lys Asp Gly Asn Asp         195 200 205 ggt act act gtg cac agc atg caa aac tac cct tgg cat ttc cac gca 672 Gly Thr Thr Val His Ser Met Gln Asn Tyr Pro Trp His Phe His Ala     210 215 220 gat att gtg aac ggg aat ata gca aaa tgc cct caa aat cat ccc tca 720 Asp Ile Val Asn Gly Asn Ile Ala Lys Cys Pro Gln Asn His Pro Ser 225 230 235 240 ggt atg gta tct caa gac ttc atg ttt cat cct atg aga gaa gaa act 768 Gly Met Val Ser Gln Asp Phe Met Phe His Pro Met Arg Glu Glu Thr                 245 250 255 cac ggg cac gca aat ctt caa gct aca aca gca tct gct act act aca 816 His Gly His Ala Asn Leu Gln Ala Thr Thr Ala Ser Ala Thr Thr Thr             260 265 270 gct tct cat caa gcg ttt cca gct tgt cat tca cag gat gat tac cgt 864 Ala Ser His Gln Ala Phe Pro Ala Cys His Ser Gln Asp Asp Tyr Arg         275 280 285 tcg ttt ctc cag ata tca tct act ttc tcc aat ctt att atg tca act 912 Ser Phe Leu Gln Ile Ser Ser Thr Phe Ser Asn Leu Ile Met Ser Thr     290 295 300 ctc cta cag aat cct gca gct cat gct gca gct aca ttc gct gct tcg 960 Leu Leu Gln Asn Pro Ala Ala His Ala Ala Ala Thr Phe Ala Ala Ser 305 310 315 320 gtc tgg cct tat gcg agt gtc ggg aat tct ggt gat tca tca acc cca 1008 Val Trp Pro Tyr Ala Ser Val Gly Asn Ser Gly Asp Ser Ser Thr Pro                 325 330 335 atg agc tct tct cct cca agt ata act gcc att gcc gct gct aca gta 1056 Met Ser Ser Ser Pro Pro Ser Ile Thr Ala Ile Ala Ala Ala Thr Val             340 345 350 Ala Ala Ala Thr Ala Trp Trp Ala Ser His Gly Leu Leu Pro Val Cys         355 360 365 gct cca gct cca ata aca tgt gtt cca ttc tca act gtt gca gtt cca 1152 Ala Pro Ala Pro Ile Thr Cys Val Pro Phe Ser Thr Val Ala Val Pro     370 375 380 act cca gca atg act gaa atg gat acc gtt gaa aat act caa ccg ttt 1200 Thr Pro Ala Met Thr Glu Met Asp Thr Val Glu Asn Thr Gln Pro Phe 385 390 395 400 gag aaa caa aac aca gct ctg caa gat caa aac ttg gct tcg aaa tct 1248 Glu Lys Gln Asn Thr Ala Leu Gln Asp Gln Asn Leu Ala Ser Lys Ser                 405 410 415 cca gct tca tca tct gat gat tca gat gag act gga gta acc aag cta 1296 Pro Ala Ser Ser Ser Asp Asp Ser Asp Glu Thr Gly Val Thr Lys Leu             420 425 430 aat gcc gac tca aaa acc aat gat gat aaa att gag gag gtt gtt gtt 1344 Asn Ala Asp Ser Lys Thr Asn Asp Asp Lys Ile Glu Glu Val Val Val         435 440 445 act gcc gct gtg cat gac tca aac act gcc cag aag aaa aat ctt gtg 1392 Thr Ala Ala Val His Asp Ser Asn Thr Ala Gln Lys Lys Asn Leu Val     450 455 460 gac cgc tca tcc tgt ggc tca aat aca cct tca ggg agt gac gca gaa 1440 Asp Arg Ser Ser Cys Gly Ser Asn Thr Pro Ser Gly Ser Asp Ala Glu 465 470 475 480 act gat gca tta gat aaa atg gag aaa gat aaa gag gat gtg aag gag 1488 Thr Asp Ala Leu Asp Lys Met Glu Lys Asp Lys Glu Asp Val Lys Glu                 485 490 495 aca gat gag aat cag cca gat gtt att gag tta aat aac cgt aag att 1536 Thr Asp Glu Asn Gln Pro Asp Val Ile Glu Leu Asn Asn Arg Lys Ile             500 505 510 aaa atg aga gac aac aac agc aac aac aat gca act act gat tcg tgg 1584 Lys Met Arg Asp Asn Asn Ser Asn Asn Asn Ala Thr Thr Asp Ser Trp         515 520 525 aag gaa gtc tcc gaa gag ggt cgt ata gcg ttt cag gct ctc ttt gca 1632 Lys Glu Val Ser Glu Glu Gly Arg Ile Ala Phe Gln Ala Leu Phe Ala     530 535 540 aga gaa aga ttg cct caa agc ttt tcg cct cct caa gtg gca gag aat 1680 Arg Glu Arg Leu Pro Gln Ser Phe Ser Pro Pro Gln Val Ala Glu Asn 545 550 555 560 gtg aat aga aaa caa agt gac acg tca atg cca ttg gct cct aat ttc 1728 Val Asn Arg Lys Gln Ser Asp Thr Ser Met Pro Leu Ala Pro Asn Phe                 565 570 575 aaa agc cag gat tct tgt gct gca gac caa gaa gga gta gta atg atc 1776 Lys Ser Gln Asp Ser Cys Ala Ala Asp Gln Glu Gly Val Val Met Ile             580 585 590 ggt gtt gga aca tgc aag agt ctt aaa acg aga cag aca gga ttt aag 1824 Gly Val Gly Thr Cys Lys Ser Leu Lys Thr Arg Gln Thr Gly Phe Lys         595 600 605 cca tac aag aga tgt tca atg gaa gtg aaa gag agc caa gtt ggg aac 1872 Pro Tyr Lys Arg Cys Ser Met Glu Val Lys Glu Ser Gln Val Gly Asn     610 615 620 ata aac aat caa agt gat gaa aaa gtc tgc aaa agg ctt cga ttg gaa 1920 Ile Asn Asn Gln Ser Asp Glu Lys Val Cys Lys Arg Leu Arg Leu Glu 625 630 635 640 gga gaa gct tct aca tga 1938 Gly Glu Ala Ser Thr                 645 <210> 44 <211> 645 <212> PRT <213> Arabidopsis thaliana <400> 44 Met Asp Thr Asn Thr Ser Gly Glu Glu Leu Leu Ala Lys Ala Arg Lys   1 5 10 15 Pro Tyr Thr Ile Thr Lys Gln Arg Glu Arg Trp Thr Glu Asp Glu His              20 25 30 Glu Arg Phe Leu Glu Ala Leu Arg Leu Tyr Gly Arg Ala Trp Gln Arg          35 40 45 Ile Glu Glu His Ile Gly Thr Lys Thr Ala Val Gln Ile Arg Ser His      50 55 60 Ala Gln Lys Phe Phe Thr Lys Leu Glu Lys Glu Ala Glu Val Lys Gly  65 70 75 80 Ile Pro Val Cys Gln Ala Leu Asp Ile Glu Ile Pro Pro Pro Arg Pro                  85 90 95 Lys Arg Lys Pro Asn Thr Pro Tyr Pro Arg Lys Pro Gly Asn Asn Gly             100 105 110 Thr Ser Ser Ser Gln Val Ser Ser Ala Lys Asp Ala Lys Leu Val Ser         115 120 125 Ser Ala Ser Ser Ser Gln Leu Asn Gln Ala Phe Leu Asp Leu Glu Lys     130 135 140 Met Pro Phe Ser Glu Lys Thr Ser Thr Gly Lys Glu Asn Gln Asp Glu 145 150 155 160 Asn Cys Ser Gly Val Ser Thr Val Asn Lys Tyr Pro Leu Pro Thr Lys                 165 170 175 Gln Val Ser Gly Asp Ile Glu Thr Ser Lys Thr Ser Thr Val Asp Asn             180 185 190 Ala Val Gln Asp Val Pro Lys Lys Asn Lys Asp Lys Asp Gly Asn Asp         195 200 205 Gly Thr Thr Val His Ser Met Gln Asn Tyr Pro Trp His Phe His Ala     210 215 220 Asp Ile Val Asn Gly Asn Ile Ala Lys Cys Pro Gln Asn His Pro Ser 225 230 235 240 Gly Met Val Ser Gln Asp Phe Met Phe His Pro Met Arg Glu Glu Thr                 245 250 255 His Gly His Ala Asn Leu Gln Ala Thr Thr Ala Ser Ala Thr Thr Thr             260 265 270 Ala Ser His Gln Ala Phe Pro Ala Cys His Ser Gln Asp Asp Tyr Arg         275 280 285 Ser Phe Leu Gln Ile Ser Ser Thr Phe Ser Asn Leu Ile Met Ser Thr     290 295 300 Leu Leu Gln Asn Pro Ala Ala His Ala Ala Ala Thr Phe Ala Ala Ser 305 310 315 320 Val Trp Pro Tyr Ala Ser Val Gly Asn Ser Gly Asp Ser Ser Thr Pro                 325 330 335 Met Ser Ser Ser Pro Pro Ser Ile Thr Ala Ile Ala Ala Ala Thr Val             340 345 350 Ala Ala Ala Thr Ala Trp Trp Ala Ser His Gly Leu Leu Pro Val Cys         355 360 365 Ala Pro Ala Pro Ile Thr Cys Val Pro Phe Ser Thr Val Ala Val Pro     370 375 380 Thr Pro Ala Met Thr Glu Met Asp Thr Val Glu Asn Thr Gln Pro Phe 385 390 395 400 Glu Lys Gln Asn Thr Ala Leu Gln Asp Gln Asn Leu Ala Ser Lys Ser                 405 410 415 Pro Ala Ser Ser Ser Asp Asp Ser Asp Glu Thr Gly Val Thr Lys Leu             420 425 430 Asn Ala Asp Ser Lys Thr Asn Asp Asp Lys Ile Glu Glu Val Val Val         435 440 445 Thr Ala Ala Val His Asp Ser Asn Thr Ala Gln Lys Lys Asn Leu Val     450 455 460 Asp Arg Ser Ser Cys Gly Ser Asn Thr Pro Ser Gly Ser Asp Ala Glu 465 470 475 480 Thr Asp Ala Leu Asp Lys Met Glu Lys Asp Lys Glu Asp Val Lys Glu                 485 490 495 Thr Asp Glu Asn Gln Pro Asp Val Ile Glu Leu Asn Asn Arg Lys Ile             500 505 510 Lys Met Arg Asp Asn Asn Ser Asn Asn Asn Ala Thr Thr Asp Ser Trp         515 520 525 Lys Glu Val Ser Glu Glu Gly Arg Ile Ala Phe Gln Ala Leu Phe Ala     530 535 540 Arg Glu Arg Leu Pro Gln Ser Phe Ser Pro Pro Gln Val Ala Glu Asn 545 550 555 560 Val Asn Arg Lys Gln Ser Asp Thr Ser Met Pro Leu Ala Pro Asn Phe                 565 570 575 Lys Ser Gln Asp Ser Cys Ala Ala Asp Gln Glu Gly Val Val Met Ile             580 585 590 Gly Val Gly Thr Cys Lys Ser Leu Lys Thr Arg Gln Thr Gly Phe Lys         595 600 605 Pro Tyr Lys Arg Cys Ser Met Glu Val Lys Glu Ser Gln Val Gly Asn     610 615 620 Ile Asn Asn Gln Ser Asp Glu Lys Val Cys Lys Arg Leu Arg Leu Glu 625 630 635 640 Gly Glu Ala Ser Thr                 645 <210> 45 <211> 1989 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (1986) <400> 45 atg aat atg agt gat tta ggt tgg gat gat gaa gat aaa tcg gtg gtt 48 Met Asn Met Ser Asp Leu Gly Trp Asp Asp Glu Asp Lys Ser Val Val   1 5 10 15 agt gct gtt tta ggg cat tta gct tct gat ttt ctt cga gca aac tct 96 Ser Ala Val Leu Gly His Leu Ala Ser Asp Phe Leu Arg Ala Asn Ser              20 25 30 aat tcg aat cag aat ctc ttt ctt gtt atg gga act gat gat act ctg 144 Asn Ser Asn Gln Asn Leu Phe Leu Val Met Gly Thr Asp Asp Thr Leu          35 40 45 aat aag aag ctc tct agt ctc gtt gat tgg cca aac tcg gag aat ttc 192 Asn Lys Lys Leu Ser Ser Leu Val Asp Trp Pro Asn Ser Glu Asn Phe      50 55 60 agc tgg aac tac gct att ttc tgg caa caa acc atg tct aga tcc gga 240 Ser Trp Asn Tyr Ala Ile Phe Trp Gln Gln Thr Met Ser Arg Ser Gly  65 70 75 80 caa caa gtc tta ggt tgg gga gat ggg tgt tgt cga gag cct aat gag 288 Gln Gln Val Leu Gly Trp Gly Asp Gly Cys Cys Arg Glu Pro Asn Glu                  85 90 95 gaa gag gaa tca aaa gtt gtt agg tct tat aat ttt aac aac atg ggg 336 Glu Glu Glu Ser Lys Val Val Arg Ser Tyr Asn Phe Asn Asn Met Gly             100 105 110 gca gag gaa gag aca tgg caa gat atg agg aag aga gtg ttg cag aag 384 Ala Glu Glu Glu Thr Trp Gln Asp Met Arg Lys Arg Val Leu Gln Lys         115 120 125 ctt cat agg ttg ttt ggt gga tct gat gaa gac aat tat gct ttg agc 432 Leu His Arg Leu Phe Gly Gly Ser Asp Glu Asp Asn Tyr Ala Leu Ser     130 135 140 tta gag aaa gtt act gct act gag att ttc ttc tta gct tcc atg tat 480 Leu Glu Lys Val Thr Ala Thr Glu Ile Phe Phe Leu Ala Ser Met Tyr 145 150 155 160 ttc ttc ttc aat cac ggt gaa ggc ggt cct ggg agg tgt tat tct tca 528 Phe Phe Phe Asn His Gly Glu Gly Gly Pro Gly Arg Cys Tyr Ser Ser                 165 170 175 ggg aaa cat gtg tgg ctc tct gat gca gtt aac tct gag tct gac tat 576 Gly Lys His Val Trp Leu Ser Asp Ala Val Asn Ser Glu Ser Asp Tyr             180 185 190 tgt ttc agg tct ttt atg gcg aaa tct gcg gga atc aga acg atc gtt 624 Cys Phe Arg Ser Phe Met Ala Lys Ser Ala Gly Ile Arg Thr Ile Val         195 200 205 atg gtt cct act gat gct ggt gtt ctt gag ctt ggt tct gtt tgg tct 672 Met Val Pro Thr Asp Ala Gly Val Leu Glu Leu Gly Ser Val Trp Ser     210 215 220 ttg cct gaa aac att ggc ttg gtt aag tct gtt caa gct ttg ttc atg 720 Leu Pro Glu Asn Ile Gly Leu Val Lys Ser Val Gln Ala Leu Phe Met 225 230 235 240 agg aga gtt acg caa cca gta atg gtg act tca aac act aac atg act 768 Arg Arg Val Thr Gln Pro Val Met Val Thr Ser Asn Thr Asn Met Thr                 245 250 255 gga ggg att cac aag ctt ttc ggg cag gat ttg agt gga gct cac gcg 816 Gly Gly Ile His Lys Leu Phe Gly Gln Asp Leu Ser Gly Ala His Ala             260 265 270 tat cct aag aag ctc gaa gtg aga aga aac ttg gat gag aga ttc act 864 Tyr Pro Lys Lys Leu Glu Val Arg Arg Asn Leu Asp Glu Arg Phe Thr         275 280 285 cct caa agt tgg gaa ggc tat aat aac aat aaa ggt cca aca ttt ggt 912 Pro Gln Ser Trp Glu Gly Tyr Asn Asn Asn Lys Gly Pro Thr Phe Gly     290 295 300 tac aca cct cag agg gat gat gtg aaa gtg cta gag aat gtg aat atg 960 Tyr Thr Pro Gln Arg Asp Asp Val Lys Val Leu Glu Asn Val Asn Met 305 310 315 320 gtt gta gat aat aac aat tac aag acg cag att gag ttt gcg gga tca 1008 Val Val Asp Asn Asn Asn Tyr Lys Thr Gln Ile Glu Phe Ala Gly Ser                 325 330 335 tca gtt gct gct tct tcg aat cca tct aca aac act cag caa gaa aaa 1056 Ser Val Ala Ala Ser Ser Asn Pro Ser Thr Asn Thr Gln Gln Glu Lys             340 345 350 tca gaa tct tgt aca gag aaa aga cca gtg agc ttg tta gca gga gca 1104 Ser Glu Ser Cys Thr Glu Lys Arg Pro Val Ser Leu Leu Ala Gly Ala         355 360 365 gga ata gtt tct gtt gtt gat gag aag aga ccg aga aag aga ggg aga 1152 Gly Ile Val Ser Val Val Asp Glu Lys Arg Pro Arg Lys Arg Gly Arg     370 375 380 aag cct gca aac gga aga gaa gag cca ttg aac cat gtg gaa gct gag 1200 Lys Pro Ala Asn Gly Arg Glu Glu Pro Leu Asn His Val Glu Ala Glu 385 390 395 400 agg cag aga cgc gag aag ctt aac caa aga ttc tac gct tta cga tca 1248 Arg Gln Arg Arg Glu Lys Leu Asn Gln Arg Phe Tyr Ala Leu Arg Ser                 405 410 415 gtt gtt cca aac att tct aaa atg gac aag gct tct cta ctt gga gac 1296 Val Val Pro Asn Ile Ser Lys Met Asp Lys Ala Ser Leu Leu Gly Asp             420 425 430 gca att tct tac atc aaa gag ctt caa gag aaa gtc aag ata atg gaa 1344 Ala Ile Ser Tyr Ile Lys Glu Leu Gln Glu Lys Val Lys Ile Met Glu         435 440 445 gat gaa aga gta gga aca gat aag agc tta tca gaa tca aac aca ata 1392 Asp Glu Arg Val Gly Thr Asp Lys Ser Leu Ser Glu Ser Asn Thr Ile     450 455 460 aca gta gaa gaa agt cca gaa gtt gac att caa gct atg aat gaa gag 1440 Thr Val Glu Glu Ser Pro Glu Val Asp Ile Gln Ala Met Asn Glu Glu 465 470 475 480 gtt gtt gta aga gta atc tcg cct ttg gat tca cat cca gct tca aga 1488 Val Val Val Arg Val Ile Ser Pro Leu Asp Ser His Pro Ala Ser Arg                 485 490 495 atc ata caa gca atg aga aac tca aat gtt agt cta atg gag gct aag 1536 Ile Ile Gln Ala Met Arg Asn Ser Asn Val Ser Leu Met Glu Ala Lys             500 505 510 tta tca tta gct gaa gac aca atg ttt cac act ttt gtg ata aag tct 1584 Leu Ser Leu Ala Glu Asp Thr Met Phe His Thr Phe Val Ile Lys Ser         515 520 525 aac aac ggg tcg gat cca ttg acg aaa gag aag ctt ata gca gcg ttt 1632 Asn Asn Gly Ser Asp Pro Leu Thr Lys Glu Lys Leu Ile Ala Ala Phe     530 535 540 tac ccc gag acc agc tcg acg caa ccg cca ttg cct tct tct agt tca 1680 Tyr Pro Glu Thr Ser Ser Thr Gln Pro Pro Leu Pro Ser Ser Ser Ser 545 550 555 560 cag gtc tct ggg ttt gtt tct ccg atc aga tcc aat ttc aat cct tct 1728 Gln Val Ser Gly Phe Val Ser Pro Ile Arg Ser Asn Phe Asn Pro Ser                 565 570 575 ccg tca gat tat ctc ctc ggc cac gat gtc tct ccg ccg cac tat cct 1776 Pro Ser Asp Tyr Leu Leu Gly His Asp Val Ser Pro Pro His Tyr Pro             580 585 590 cga tct cca ccg tca aac cca gag agc cac tct ctc aaa gtc tct acc 1824 Arg Ser Pro Pro Ser Asn Pro Glu Ser His Ser Leu Lys Val Ser Thr         595 600 605 ttt ctc gat gag tca cat ctc ctc cgc atc cgc cac cgc cgc cgt gag 1872 Phe Leu Asp Glu Ser His Leu Leu Arg Ile Arg His Arg Arg Arg Glu     610 615 620 aaa ccc act ggg aag aga cct ctc ttc aat ccc ctt cgc aca agc cca 1920 Lys Pro Thr Gly Lys Arg Pro Leu Phe Asn Pro Leu Arg Thr Ser Pro 625 630 635 640 gaa act gaa acc aga ttc aac caa tct cgt cac cga tcg ctc gat ttc 1968 Glu Thr Glu Thr Arg Phe Asn Gln Ser Arg His Arg Ser Leu Asp Phe                 645 650 655 gtc ttc aat tgg gca atc tga 1989 Val Phe Asn Trp Ala Ile             660 <210> 46 <211> 662 <212> PRT <213> Arabidopsis thaliana <400> 46 Met Asn Met Ser Asp Leu Gly Trp Asp Asp Glu Asp Lys Ser Val Val   1 5 10 15 Ser Ala Val Leu Gly His Leu Ala Ser Asp Phe Leu Arg Ala Asn Ser              20 25 30 Asn Ser Asn Gln Asn Leu Phe Leu Val Met Gly Thr Asp Asp Thr Leu          35 40 45 Asn Lys Lys Leu Ser Ser Leu Val Asp Trp Pro Asn Ser Glu Asn Phe      50 55 60 Ser Trp Asn Tyr Ala Ile Phe Trp Gln Gln Thr Met Ser Arg Ser Gly  65 70 75 80 Gln Gln Val Leu Gly Trp Gly Asp Gly Cys Cys Arg Glu Pro Asn Glu                  85 90 95 Glu Glu Glu Ser Lys Val Val Arg Ser Tyr Asn Phe Asn Asn Met Gly             100 105 110 Ala Glu Glu Glu Thr Trp Gln Asp Met Arg Lys Arg Val Leu Gln Lys         115 120 125 Leu His Arg Leu Phe Gly Gly Ser Asp Glu Asp Asn Tyr Ala Leu Ser     130 135 140 Leu Glu Lys Val Thr Ala Thr Glu Ile Phe Phe Leu Ala Ser Met Tyr 145 150 155 160 Phe Phe Phe Asn His Gly Glu Gly Gly Pro Gly Arg Cys Tyr Ser Ser                 165 170 175 Gly Lys His Val Trp Leu Ser Asp Ala Val Asn Ser Glu Ser Asp Tyr             180 185 190 Cys Phe Arg Ser Phe Met Ala Lys Ser Ala Gly Ile Arg Thr Ile Val         195 200 205 Met Val Pro Thr Asp Ala Gly Val Leu Glu Leu Gly Ser Val Trp Ser     210 215 220 Leu Pro Glu Asn Ile Gly Leu Val Lys Ser Val Gln Ala Leu Phe Met 225 230 235 240 Arg Arg Val Thr Gln Pro Val Met Val Thr Ser Asn Thr Asn Met Thr                 245 250 255 Gly Gly Ile His Lys Leu Phe Gly Gln Asp Leu Ser Gly Ala His Ala             260 265 270 Tyr Pro Lys Lys Leu Glu Val Arg Arg Asn Leu Asp Glu Arg Phe Thr         275 280 285 Pro Gln Ser Trp Glu Gly Tyr Asn Asn Asn Lys Gly Pro Thr Phe Gly     290 295 300 Tyr Thr Pro Gln Arg Asp Asp Val Lys Val Leu Glu Asn Val Asn Met 305 310 315 320 Val Val Asp Asn Asn Asn Tyr Lys Thr Gln Ile Glu Phe Ala Gly Ser                 325 330 335 Ser Val Ala Ala Ser Ser Asn Pro Ser Thr Asn Thr Gln Gln Glu Lys             340 345 350 Ser Glu Ser Cys Thr Glu Lys Arg Pro Val Ser Leu Leu Ala Gly Ala         355 360 365 Gly Ile Val Ser Val Val Asp Glu Lys Arg Pro Arg Lys Arg Gly Arg     370 375 380 Lys Pro Ala Asn Gly Arg Glu Glu Pro Leu Asn His Val Glu Ala Glu 385 390 395 400 Arg Gln Arg Arg Glu Lys Leu Asn Gln Arg Phe Tyr Ala Leu Arg Ser                 405 410 415 Val Val Pro Asn Ile Ser Lys Met Asp Lys Ala Ser Leu Leu Gly Asp             420 425 430 Ala Ile Ser Tyr Ile Lys Glu Leu Gln Glu Lys Val Lys Ile Met Glu         435 440 445 Asp Glu Arg Val Gly Thr Asp Lys Ser Leu Ser Glu Ser Asn Thr Ile     450 455 460 Thr Val Glu Glu Ser Pro Glu Val Asp Ile Gln Ala Met Asn Glu Glu 465 470 475 480 Val Val Val Arg Val Ile Ser Pro Leu Asp Ser His Pro Ala Ser Arg                 485 490 495 Ile Ile Gln Ala Met Arg Asn Ser Asn Val Ser Leu Met Glu Ala Lys             500 505 510 Leu Ser Leu Ala Glu Asp Thr Met Phe His Thr Phe Val Ile Lys Ser         515 520 525 Asn Asn Gly Ser Asp Pro Leu Thr Lys Glu Lys Leu Ile Ala Ala Phe     530 535 540 Tyr Pro Glu Thr Ser Ser Thr Gln Pro Pro Leu Pro Ser Ser Ser Ser 545 550 555 560 Gln Val Ser Gly Phe Val Ser Pro Ile Arg Ser Asn Phe Asn Pro Ser                 565 570 575 Pro Ser Asp Tyr Leu Leu Gly His Asp Val Ser Pro Pro His Tyr Pro             580 585 590 Arg Ser Pro Pro Ser Asn Pro Glu Ser His Ser Leu Lys Val Ser Thr         595 600 605 Phe Leu Asp Glu Ser His Leu Leu Arg Ile Arg His Arg Arg Arg Glu     610 615 620 Lys Pro Thr Gly Lys Arg Pro Leu Phe Asn Pro Leu Arg Thr Ser Pro 625 630 635 640 Glu Thr Glu Thr Arg Phe Asn Gln Ser Arg His Arg Ser Leu Asp Phe                 645 650 655 Val Phe Asn Trp Ala Ile             660 <210> 47 <211> 870 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (867) <400> 47 atg tca gaa tta tta cag ttg cct cca ggt ttc cga ttt cac cct acc 48 Met Ser Glu Leu Leu Gln Leu Pro Pro Gly Phe Arg Phe His Pro Thr   1 5 10 15 gat gaa gag ctt gtc atg cac tat ctc tgc cgc aaa tgt gcc tct cag 96 Asp Glu Glu Leu Val Met His Tyr Leu Cys Arg Lys Cys Ala Ser Gln              20 25 30 tcc atc gcc gtt ccg atc atc gct gag atc gat ctc tac aaa tac gat 144 Ser Ile Ala Val Pro Ile Ile Ala Glu Ile Asp Leu Tyr Lys Tyr Asp          35 40 45 cca tgg gag ctt cct ggt tta gcc ttg tat ggt gag aag gaa tgg tac 192 Pro Trp Glu Leu Pro Gly Leu Ala Leu Tyr Gly Glu Lys Glu Trp Tyr      50 55 60 ttc ttc tct ccc agg gac aga aaa tat ccc aac ggt tcg cgt cct aac 240 Phe Phe Ser Pro Arg Asp Arg Lys Tyr Pro Asn Gly Ser Arg Pro Asn  65 70 75 80 cgg tcc gct ggt tct ggt tac tgg aaa gct acc gga gct gat aaa ccg 288 Arg Ser Ala Gly Ser Gly Tyr Trp Lys Ala Thr Gly Ala Asp Lys Pro                  85 90 95 atc gga cta cct aaa ccg gtc gga att aag aaa gct ctt gtt ttc tac 336 Ile Gly Leu Pro Lys Pro Val Gly Ile Lys Lys Ala Leu Val Phe Tyr             100 105 110 gcc ggc aaa gct cca aag gga gag aaa acc aat tgg atc atg cac gag 384 Ala Gly Lys Ala Pro Lys Gly Glu Lys Thr Asn Trp Ile Met His Glu         115 120 125 tac cgt ctc gcc gac gtt gac cgg tcc gtt cgc aag aag aag aat agt 432 Tyr Arg Leu Ala Asp Val Asp Arg Ser Val Arg Lys Lys Lys Asn Ser     130 135 140 ctc agg ctg gat gat tgg gtt ctc tgc cgg att tac aac aaa aaa gga 480 Leu Arg Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr Asn Lys Lys Gly 145 150 155 160 gct acc gag agg cgg gga cca ccg cct ccg gtt gtt tac ggc gac gaa 528 Ala Thr Glu Arg Arg Gly Pro Pro Pro Pro Val Val Tyr Gly Asp Glu                 165 170 175 atc atg gag gag aag ccg aag gtg acg gag atg gtt atg cct ccg ccg 576 Ile Met Glu Glu Lys Pro Lys Val Thr Glu Met Val Met Pro Pro Pro             180 185 190 ccg caa cag aca agt gag ttc gcg tat ttc gac acg tcg gat tcg gtg 624 Pro Gln Gln Thr Ser Glu Phe Ala Tyr Phe Asp Thr Ser Asp Ser Val         195 200 205 ccg aag ctg cat act acg gat tcg agt tgc tcg gag cag gtg gtg tcg 672 Pro Lys Leu His Thr Thr Asp Ser Ser Cys Ser Glu Gln Val Val Ser     210 215 220 ccg gag ttc acg agc gag gtt cag agc gag ccc aag tgg aaa gat tgg 720 Pro Glu Phe Thr Ser Glu Val Gln Ser Glu Pro Lys Trp Lys Asp Trp 225 230 235 240 tcg gcc gta agt aat gac aat aac aat acc ctt gat ttt ggg ttt aat 768 Ser Ala Val Ser Asn Asp Asn Asn Asn Thr Leu Asp Phe Gly Phe Asn                 245 250 255 tac att gat gcc acc gtg gat aac gcg ttt gga gga gga ggg agt agt 816 Tyr Ile Asp Ala Thr Val Asp Asn Ala Phe Gly Gly Gly Gly Ser Ser             260 265 270 aat cag atg ttt ccg cta cag gat atg ttc atg tac atg cag aag cct 864 Asn Gln Met Phe Pro Leu Gln Asp Met Phe Met Tyr Met Gln Lys Pro         275 280 285 tac tag 870 Tyr <210> 48 <211> 289 <212> PRT <213> Arabidopsis thaliana <400> 48 Met Ser Glu Leu Leu Gln Leu Pro Pro Gly Phe Arg Phe His Pro Thr   1 5 10 15 Asp Glu Glu Leu Val Met His Tyr Leu Cys Arg Lys Cys Ala Ser Gln              20 25 30 Ser Ile Ala Val Pro Ile Ile Ala Glu Ile Asp Leu Tyr Lys Tyr Asp          35 40 45 Pro Trp Glu Leu Pro Gly Leu Ala Leu Tyr Gly Glu Lys Glu Trp Tyr      50 55 60 Phe Phe Ser Pro Arg Asp Arg Lys Tyr Pro Asn Gly Ser Arg Pro Asn  65 70 75 80 Arg Ser Ala Gly Ser Gly Tyr Trp Lys Ala Thr Gly Ala Asp Lys Pro                  85 90 95 Ile Gly Leu Pro Lys Pro Val Gly Ile Lys Lys Ala Leu Val Phe Tyr             100 105 110 Ala Gly Lys Ala Pro Lys Gly Glu Lys Thr Asn Trp Ile Met His Glu         115 120 125 Tyr Arg Leu Ala Asp Val Asp Arg Ser Val Arg Lys Lys Lys Asn Ser     130 135 140 Leu Arg Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr Asn Lys Lys Gly 145 150 155 160 Ala Thr Glu Arg Arg Gly Pro Pro Pro Pro Val Val Tyr Gly Asp Glu                 165 170 175 Ile Met Glu Glu Lys Pro Lys Val Thr Glu Met Val Met Pro Pro Pro             180 185 190 Pro Gln Gln Thr Ser Glu Phe Ala Tyr Phe Asp Thr Ser Asp Ser Val         195 200 205 Pro Lys Leu His Thr Thr Asp Ser Ser Cys Ser Glu Gln Val Val Ser     210 215 220 Pro Glu Phe Thr Ser Glu Val Gln Ser Glu Pro Lys Trp Lys Asp Trp 225 230 235 240 Ser Ala Val Ser Asn Asp Asn Asn Asn Thr Leu Asp Phe Gly Phe Asn                 245 250 255 Tyr Ile Asp Ala Thr Val Asp Asn Ala Phe Gly Gly Gly Gly Ser Ser             260 265 270 Asn Gln Met Phe Pro Leu Gln Asp Met Phe Met Tyr Met Gln Lys Pro         275 280 285 Tyr <210> 49 <211> 1202 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (112) .. (918) <400> 49 tcctcgattt caatctttga gataaaccac aaagatcctc cgattcgaag gtttataaaa 60 actcaaaatc gaatcttatc cacaagaaaa caacaaggta cttttccaaa a atg aag 117                                                          Met Lys                                                            1 gcg gag ttg aat ttg ccg gcg gga ttc cga ttt cat ccg acg gac gaa 165 Ala Glu Leu Asn Leu Pro Ala Gly Phe Arg Phe His Pro Thr Asp Glu           5 10 15 gag ctt gtc aag ttc tat ctt tgc cgg aga tgt gcg tca gaa ccg att 213 Glu Leu Val Lys Phe Tyr Leu Cys Arg Arg Cys Ala Ser Glu Pro Ile      20 25 30 aac gtt ccg gtt atc gca gag att gac ttg tac aaa ttc aat cca agg 261 Asn Val Pro Val Ile Ala Glu Ile Asp Leu Tyr Lys Phe Asn Pro Arg  35 40 45 50 gag ctt cca gaa atg gcg ttg tac ggt gag aaa gaa tgg tac ttc ttc 309 Glu Leu Pro Glu Met Ala Leu Tyr Gly Glu Lys Glu Trp Tyr Phe Phe                  55 60 65 tcg cat aga gac cgg aaa tac cca aac ggg tcg aga cca aac cgg gca 357 Ser His Arg Asp Arg Lys Tyr Pro Asn Gly Ser Arg Pro Asn Arg Ala              70 75 80 gct gga acc ggt tat tgg aaa gcg act gga gct gat aaa ccg atc gga 405 Ala Gly Thr Gly Tyr Trp Lys Ala Thr Gly Ala Asp Lys Pro Ile Gly          85 90 95 aaa ccg aag acg tta ggg att aag aaa gca ctc gtc ttc tac gca gga 453 Lys Pro Lys Thr Leu Gly Ile Lys Lys Ala Leu Val Phe Tyr Ala Gly     100 105 110 aaa gct ccg aaa ggg att aaa acg aat tgg att atg cac gag tat cgt 501 Lys Ala Pro Lys Gly Ile Lys Thr Asn Trp Ile Met His Glu Tyr Arg 115 120 125 130 ctc gct aat gtc gat cga tct gct tct acc aac aag aag aac aac tta 549 Leu Ala Asn Val Asp Arg Ser Ala Ser Thr Asn Lys Lys Asn Asn Leu                 135 140 145 aga ctt gat gat tgg gtt ttg tgt cgg ata tac aat aag aaa gga aca 597 Arg Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr Asn Lys Lys Gly Thr             150 155 160 atg gag aag tat tta ccg gcg gcg gct gag aaa ccg acg gaa aag atg 645 Met Glu Lys Tyr Leu Pro Ala Ala Ala Glu Lys Pro Thr Glu Lys Met         165 170 175 agt acg tcg gac tca aga tgc tca agt cac gtg att tca ccg gac gtc 693 Ser Thr Ser Asp Ser Arg Cys Ser Ser His Val Ile Ser Pro Asp Val     180 185 190 acg tgt tct gat aac tgg gag gtt gag agt gag ccc aaa tgg att aat 741 Thr Cys Ser Asp Asn Trp Glu Val Glu Ser Glu Pro Lys Trp Ile Asn 195 200 205 210 ctg gaa gac gcg tta gag gca ttt aat gat gac acg tcc atg ttt agt 789 Leu Glu Asp Ala Leu Glu Ala Phe Asn Asp Asp Thr Ser Met Phe Ser                 215 220 225 tcc att ggt ttg ttg caa aat gac gcc ttt gtt cct cag ttt cag tac 837 Ser Ile Gly Leu Leu Gln Asn Asp Ala Phe Val Pro Gln Phe Gln Tyr             230 235 240 cag tcc tcc gat ttc gtc gat tcg ttt cag gac ccg ttc gag cag aaa 885 Gln Ser Ser Asp Phe Val Asp Ser Phe Gln Asp Pro Phe Glu Gln Lys         245 250 255 ccg ttc ttg aat tgg aat ttt gct cct caa ggg taaaaataat cggcaaaaag 938 Pro Phe Leu Asn Trp Asn Phe Ala Pro Gln Gly     260 265 ttgaagcttt tcagagtctt cgatcaccgg cattgtgtcg gatcctgacc cggagaccaa 998 gtcgggtcat acgattacat aatcgggtta ttgagatttc cacatttgga tttccgagac 1058 taaccaactt aacggattct ggggtaattg gggggttttg cacaggtgaa tcacactgag 1118 tcagcaagtt tcgatttttt ggttttgttt tgtaatgatt gattaaatgt ctaaagatat 1178 cacgaagtta aaaaaaaaaa aaaa 1202 <210> 50 <211> 269 <212> PRT <213> Arabidopsis thaliana <400> 50 Met Lys Ala Glu Leu Asn Leu Pro Ala Gly Phe Arg Phe His Pro Thr   1 5 10 15 Asp Glu Glu Leu Val Lys Phe Tyr Leu Cys Arg Arg Cys Ala Ser Glu              20 25 30 Pro Ile Asn Val Pro Val Ile Ala Glu Ile Asp Leu Tyr Lys Phe Asn          35 40 45 Pro Arg Glu Leu Pro Glu Met Ala Leu Tyr Gly Glu Lys Glu Trp Tyr      50 55 60 Phe Phe Ser His Arg Asp Arg Lys Tyr Pro Asn Gly Ser Arg Pro Asn  65 70 75 80 Arg Ala Ala Gly Thr Gly Tyr Trp Lys Ala Thr Gly Ala Asp Lys Pro                  85 90 95 Ile Gly Lys Pro Lys Thr Leu Gly Ile Lys Lys Ala Leu Val Phe Tyr             100 105 110 Ala Gly Lys Ala Pro Lys Gly Ile Lys Thr Asn Trp Ile Met His Glu         115 120 125 Tyr Arg Leu Ala Asn Val Asp Arg Ser Ala Ser Thr Asn Lys Lys Asn     130 135 140 Asn Leu Arg Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr Asn Lys Lys 145 150 155 160 Gly Thr Met Glu Lys Tyr Leu Pro Ala Ala Ala Glu Lys Pro Thr Glu                 165 170 175 Lys Met Ser Thr Ser Asp Ser Arg Cys Ser Ser His Val Ile Ser Pro             180 185 190 Asp Val Thr Cys Ser Asp Asn Trp Glu Val Glu Ser Glu Pro Lys Trp         195 200 205 Ile Asn Leu Glu Asp Ala Leu Glu Ala Phe Asn Asp Asp Thr Ser Met     210 215 220 Phe Ser Ser Ile Gly Leu Leu Gln Asn Asp Ala Phe Val Pro Gln Phe 225 230 235 240 Gln Tyr Gln Ser Ser Asp Phe Val Asp Ser Phe Gln Asp Pro Phe Glu                 245 250 255 Gln Lys Pro Phe Leu Asn Trp Asn Phe Ala Pro Gln Gly             260 265 <210> 51 <211> 1188 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (124) .. (978) <400> 51 aaaaacctca actttcttct ctcttctcaa aaacccttcc ctcttcgtct ccaaacaaca 60 acaaacacaa caacaacaaa aatcttacaa gaagatcatt tttagaaacc ctattaggat 120 aaa atg gat tac gag gca tca aga atc gtc gaa atg gta gaa gat gaa 168     Met Asp Tyr Glu Ala Ser Arg Ile Val Glu Met Val Glu Asp Glu       1 5 10 15 gaa cat ata gat cta cca cca gga ttc aga ttt cac cct act gat gaa 216 Glu His Ile Asp Leu Pro Pro Gly Phe Arg Phe His Pro Thr Asp Glu                  20 25 30 gaa ctc ata act cac tac ctc aaa cca aag gtt ttc aac act ttc ttc 264 Glu Leu Ile Thr His Tyr Leu Lys Pro Lys Val Phe Asn Thr Phe Phe              35 40 45 tct gct act gcc att ggt gaa gtt gat ctc aac aag att gag cct tgg 312 Ser Ala Thr Ala Ile Gly Glu Val Asp Leu Asn Lys Ile Glu Pro Trp          50 55 60 gac tta cca tgg aag gct aag atg gga gaa aaa gaa tgg tat ttc ttc 360 Asp Leu Pro Trp Lys Ala Lys Met Gly Glu Lys Glu Trp Tyr Phe Phe      65 70 75 tgt gtg aga gac cgg aaa tac ccg acc ggt tta agg aca aac cgg gcg 408 Cys Val Arg Asp Arg Lys Tyr Pro Thr Gly Leu Arg Thr Asn Arg Ala  80 85 90 95 aca gaa gcc ggt tat tgg aaa gcc aca gga aaa gac aaa gag ata ttc 456 Thr Glu Ala Gly Tyr Trp Lys Ala Thr Gly Lys Asp Lys Glu Ile Phe                 100 105 110 aag gga aaa tca ctt gtg ggt atg aag aaa act ttg gtt ttc tat aaa 504 Lys Gly Lys Ser Leu Val Gly Met Lys Lys Thr Leu Val Phe Tyr Lys             115 120 125 gga aga gct cct aaa gga gtt aaa acc aat tgg gtt atg cat gaa tat 552 Gly Arg Ala Pro Lys Gly Val Lys Thr Asn Trp Val Met His Glu Tyr         130 135 140 cgt tta gaa ggc aaa tat tgt att gaa aat ctt ccc caa aca gct aag 600 Arg Leu Glu Gly Lys Tyr Cys Ile Glu Asn Leu Pro Gln Thr Ala Lys     145 150 155 aac gaa tgg gtt ata tgt cgt gtt ttc caa aaa cgt gcc gat ggt aca 648 Asn Glu Trp Val Ile Cys Arg Val Phe Gln Lys Arg Ala Asp Gly Thr 160 165 170 175 aag gtt cca atg tca atg ctt gat cca cac att aac cga atg gaa cca 696 Lys Val Pro Met Ser Met Leu Asp Pro His Ile Asn Arg Met Glu Pro                 180 185 190 gcc ggt tta cct tcg tta atg gat tgt tct caa cga gac tcc ttc acc 744 Ala Gly Leu Pro Ser Leu Met Asp Cys Ser Gln Arg Asp Ser Phe Thr             195 200 205 ggt tcg tcg tct cac gtg acc tgc ttc tcc gac caa gaa acc gaa gac 792 Gly Ser Ser Ser His Val Thr Cys Phe Ser Asp Gln Glu Thr Glu Asp         210 215 220 aaa aga ctt gtc cac gag tcc aaa gac ggt ttt ggt tct ctg ttt tac 840 Lys Arg Leu Val His Glu Ser Lys Asp Gly Phe Gly Ser Leu Phe Tyr     225 230 235 tcg gat cct ctg ttt tta caa gac aat tat tcg cta atg aag ctg ttg 888 Ser Asp Pro Leu Phe Leu Gln Asp Asn Tyr Ser Leu Met Lys Leu Leu 240 245 250 255 ctt gac ggt caa gaa act caa ttc tcc ggc aaa cct ttc gac ggt cgt 936 Leu Asp Gly Gln Glu Thr Gln Phe Ser Gly Lys Pro Phe Asp Gly Arg                 260 265 270 gat tcg tcc ggt aca gaa gaa ttg gat tgc gtt tgg aat ttc 978 Asp Ser Ser Gly Thr Glu Glu Leu Asp Cys Val Trp Asn Phe             275 280 285 tgagttgtat aagttatgtt gtagacttgt agtagtcatg tgttcgtgtg tgtgaatgaa 1038 tattcttgtt acattttttt gtaaaaaagg agaaaaaaat atgctagaaa gtcaattgct 1098 tttgttatgt agcattagtg ttttttatgt actcaataga cttcctaatt aaataaaaat 1158 cttaatttat ttgcaaaaaa aaaaaaaaaa 1188 <210> 52 <211> 285 <212> PRT <213> Arabidopsis thaliana <400> 52 Met Asp Tyr Glu Ala Ser Arg Ile Val Glu Met Val Glu Asp Glu Glu   1 5 10 15 His Ile Asp Leu Pro Pro Gly Phe Arg Phe His Pro Thr Asp Glu Glu              20 25 30 Leu Ile Thr His Tyr Leu Lys Pro Lys Val Phe Asn Thr Phe Phe Ser          35 40 45 Ala Thr Ala Ile Gly Glu Val Asp Leu Asn Lys Ile Glu Pro Trp Asp      50 55 60 Leu Pro Trp Lys Ala Lys Met Gly Glu Lys Glu Trp Tyr Phe Phe Cys  65 70 75 80 Val Arg Asp Arg Lys Tyr Pro Thr Gly Leu Arg Thr Asn Arg Ala Thr                  85 90 95 Glu Ala Gly Tyr Trp Lys Ala Thr Gly Lys Asp Lys Glu Ile Phe Lys             100 105 110 Gly Lys Ser Leu Val Gly Met Lys Lys Thr Leu Val Phe Tyr Lys Gly         115 120 125 Arg Ala Pro Lys Gly Val Lys Thr Asn Trp Val Met His Glu Tyr Arg     130 135 140 Leu Glu Gly Lys Tyr Cys Ile Glu Asn Leu Pro Gln Thr Ala Lys Asn 145 150 155 160 Glu Trp Val Ile Cys Arg Val Phe Gln Lys Arg Ala Asp Gly Thr Lys                 165 170 175 Val Pro Met Ser Met Leu Asp Pro His Ile Asn Arg Met Glu Pro Ala             180 185 190 Gly Leu Pro Ser Leu Met Asp Cys Ser Gln Arg Asp Ser Phe Thr Gly         195 200 205 Ser Ser Ser His Val Thr Cys Phe Ser Asp Gln Glu Thr Glu Asp Lys     210 215 220 Arg Leu Val His Glu Ser Lys Asp Gly Phe Gly Ser Leu Phe Tyr Ser 225 230 235 240 Asp Pro Leu Phe Leu Gln Asp Asn Tyr Ser Leu Met Lys Leu Leu Leu                 245 250 255 Asp Gly Gln Glu Thr Gln Phe Ser Gly Lys Pro Phe Asp Gly Arg Asp             260 265 270 Ser Ser Gly Thr Glu Glu Leu Asp Cys Val Trp Asn Phe         275 280 285 <210> 53 <211> 894 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (891) <400> 53 atg ggt gtt aga gag aaa gat ccg tta gcc cag ttg agt ttg cca cca 48 Met Gly Val Arg Glu Lys Asp Pro Leu Ala Gln Leu Ser Leu Pro Pro   1 5 10 15 ggt ttt aga ttt tat ccg aca gat gaa gag ctt ctt gtt cag tat cta 96 Gly Phe Arg Phe Tyr Pro Thr Asp Glu Glu Leu Leu Val Gln Tyr Leu              20 25 30 tgt cgg aaa gtt gca ggc tat cat ttc tct ctc cag gtc atc gga gac 144 Cys Arg Lys Val Ala Gly Tyr His Phe Ser Leu Gln Val Ile Gly Asp          35 40 45 atc gat ctc tac aag ttc gat cct tgg gat ttg cca agt aag gct ttg 192 Ile Asp Leu Tyr Lys Phe Asp Pro Trp Asp Leu Pro Ser Lys Ala Leu      50 55 60 ttt gga gag aag gaa tgg tat ttc ttt agc cca aga gat cgg aaa tat 240 Phe Gly Glu Lys Glu Trp Tyr Phe Phe Ser Pro Arg Asp Arg Lys Tyr  65 70 75 80 ccg aac ggg tca aga ccc aat aga gta gcc ggg tcg ggt tat tgg aaa 288 Pro Asn Gly Ser Arg Pro Asn Arg Val Ala Gly Ser Gly Tyr Trp Lys                  85 90 95 gca acg ggt act gac aaa att atc acg gcg gat ggt cgt cgt gtc ggg 336 Ala Thr Gly Thr Asp Lys Ile Ile Thr Ala Asp Gly Arg Arg Val Gly             100 105 110 att aaa aaa gct ctg gtc ttt tac gcc gga aaa gct ccc aaa ggc act 384 Ile Lys Lys Ala Leu Val Phe Tyr Ala Gly Lys Ala Pro Lys Gly Thr         115 120 125 aaa acc aac tgg att atg cac gag tat cgc tta ata gaa cat tct cgt 432 Lys Thr Asn Trp Ile Met His Glu Tyr Arg Leu Ile Glu His Ser Arg     130 135 140 agc cat gga agc tcc aag ttg gat gat tgg gtg ttg tgt cga att tac 480 Ser His Gly Ser Ser Lys Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr 145 150 155 160 aag aaa aca tct gga tct cag aga caa gct gtt act cct gtt caa gct 528 Lys Lys Thr Ser Gly Ser Gln Arg Gln Ala Val Thr Pro Val Gln Ala                 165 170 175 tgt cgt gaa gag cat agc acg aat ggg tcg tca tcg tct tct tca tca 576 Cys Arg Glu Glu His Ser Thr Asn Gly Ser Ser Ser Ser Ser Ser Ser Ser             180 185 190 cag ctt gac gac gtt ctt gat tcg ttc ccg gag ata aaa gac cag tct 624 Gln Leu Asp Asp Val Leu Asp Ser Phe Pro Glu Ile Lys Asp Gln Ser         195 200 205 ttt aat ctt cct cgg atg aat tcg ctc agg acg att ctt aac ggg aac 672 Phe Asn Leu Pro Arg Met Asn Ser Leu Arg Thr Ile Leu Asn Gly Asn     210 215 220 ttt gat tgg gct agc ttg gca ggt ctt aat cca att cca gag cta gct 720 Phe Asp Trp Ala Ser Leu Ala Gly Leu Asn Pro Ile Pro Glu Leu Ala 225 230 235 240 ccg acc aat gga tta ccg agt tac ggt ggt tac gat gcg ttt cga gcg 768 Pro Thr Asn Gly Leu Pro Ser Tyr Gly Gly Tyr Asp Ala Phe Arg Ala                 245 250 255 gcg gaa ggt gag gcg gag agt ggg cat gtg aat cgg cag cag aac tcg 816 Ala Glu Gly Glu Ala Glu Ser Gly His Val Asn Arg Gln Gln Asn Ser             260 265 270 agc ggg ttg act cag agt ttc ggg tac agc tcg agt ggg ttt ggt gtt 864 Ser Gly Leu Thr Gln Ser Phe Gly Tyr Ser Ser Ser Gly Phe Gly Val         275 280 285 tcg ggt caa aca ttc gag ttt agg caa tga 894 Ser Gly Gln Thr Phe Glu Phe Arg Gln     290 295 <210> 54 <211> 297 <212> PRT <213> Arabidopsis thaliana <400> 54 Met Gly Val Arg Glu Lys Asp Pro Leu Ala Gln Leu Ser Leu Pro Pro   1 5 10 15 Gly Phe Arg Phe Tyr Pro Thr Asp Glu Glu Leu Leu Val Gln Tyr Leu              20 25 30 Cys Arg Lys Val Ala Gly Tyr His Phe Ser Leu Gln Val Ile Gly Asp          35 40 45 Ile Asp Leu Tyr Lys Phe Asp Pro Trp Asp Leu Pro Ser Lys Ala Leu      50 55 60 Phe Gly Glu Lys Glu Trp Tyr Phe Phe Ser Pro Arg Asp Arg Lys Tyr  65 70 75 80 Pro Asn Gly Ser Arg Pro Asn Arg Val Ala Gly Ser Gly Tyr Trp Lys                  85 90 95 Ala Thr Gly Thr Asp Lys Ile Ile Thr Ala Asp Gly Arg Arg Val Gly             100 105 110 Ile Lys Lys Ala Leu Val Phe Tyr Ala Gly Lys Ala Pro Lys Gly Thr         115 120 125 Lys Thr Asn Trp Ile Met His Glu Tyr Arg Leu Ile Glu His Ser Arg     130 135 140 Ser His Gly Ser Ser Lys Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr 145 150 155 160 Lys Lys Thr Ser Gly Ser Gln Arg Gln Ala Val Thr Pro Val Gln Ala                 165 170 175 Cys Arg Glu Glu His Ser Thr Asn Gly Ser Ser Ser Ser Ser Ser Ser Ser             180 185 190 Gln Leu Asp Asp Val Leu Asp Ser Phe Pro Glu Ile Lys Asp Gln Ser         195 200 205 Phe Asn Leu Pro Arg Met Asn Ser Leu Arg Thr Ile Leu Asn Gly Asn     210 215 220 Phe Asp Trp Ala Ser Leu Ala Gly Leu Asn Pro Ile Pro Glu Leu Ala 225 230 235 240 Pro Thr Asn Gly Leu Pro Ser Tyr Gly Gly Tyr Asp Ala Phe Arg Ala                 245 250 255 Ala Glu Gly Glu Ala Glu Ser Gly His Val Asn Arg Gln Gln Asn Ser             260 265 270 Ser Gly Leu Thr Gln Ser Phe Gly Tyr Ser Ser Ser Gly Phe Gly Val         275 280 285 Ser Gly Gln Thr Phe Glu Phe Arg Gln     290 295 <210> 55 <211> 1920 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (62) .. (1645) <400> 55 acagaaaaat cgaaactttt tagggttttt tttttttgtg ataacgagag agaaaaaagt 60 g atg gac ttg tcg gtt gag aac gga ggt tta gct cca ggt ttt agg ttt 109   Met Asp Leu Ser Val Glu Asn Gly Gly Leu Ala Pro Gly Phe Arg Phe     1 5 10 15 cat ccg acg gac gaa gaa ctt gtc gtc tat tat ctc aaa aga aag atc 157 His Pro Thr Asp Glu Glu Leu Val Val Tyr Tyr Leu Lys Arg Lys Ile              20 25 30 cgt cgg aaa aaa ctc aga gtc gaa gca atc ggc gag act gat gtc tat 205 Arg Arg Lys Lys Leu Arg Val Glu Ala Ile Gly Glu Thr Asp Val Tyr          35 40 45 aag ttt gat cct gag gaa tta cct gag aaa gcg ttg tat aag act aga 253 Lys Phe Asp Pro Glu Glu Leu Pro Glu Lys Ala Leu Tyr Lys Thr Arg      50 55 60 gat cgt caa tgg ttc ttt ttc agc tta agg gat agg aaa cat gga agt 301 Asp Arg Gln Trp Phe Phe Phe Ser Leu Arg Asp Arg Lys His Gly Ser  65 70 75 80 agg tca agt aga gct act gaa cgt ggc tat tgg aaa gca aca ggg aag 349 Arg Ser Ser Arg Ala Thr Glu Arg Gly Tyr Trp Lys Ala Thr Gly Lys                  85 90 95 gat aga gtc att cat tgt gat tcg aga ccc gtt gga gag aag aag act 397 Asp Arg Val Ile His Cys Asp Ser Arg Pro Val Gly Glu Lys Lys Thr             100 105 110 ctt gtt ttc cat aga ggc agg gca cct aat ggc gaa cgg act aat tgg 445 Leu Val Phe His Arg Gly Arg Ala Pro Asn Gly Glu Arg Thr Asn Trp         115 120 125 gtg atg cat gag tat aca ttg cac aaa gag gag ctc aag agg tgt ggt 493 Val Met His Glu Tyr Thr Leu His Lys Glu Glu Leu Lys Arg Cys Gly     130 135 140 ggt gaa gat gtt aag gat gct tat gtt ctt tac aag att tat aag aaa 541 Gly Glu Asp Val Lys Asp Ala Tyr Val Leu Tyr Lys Ile Tyr Lys Lys 145 150 155 160 agt ggg tct ggt cct aag aat ggt gag caa tat gga gct cct ttt att 589 Ser Gly Ser Gly Pro Lys Asn Gly Glu Gln Tyr Gly Ala Pro Phe Ile                 165 170 175 gaa gaa gaa tgg gct gaa gat gat gat gat gat gtt gat gag cct gct 637 Glu Glu Glu Trp Ala Glu Asp Asp Asp Asp Asp Val Asp Glu Pro Ala             180 185 190 aat cag ctc gtt gtt tcg gct agt gtt gat aat agt tta tgg ggg aaa 685 Asn Gln Leu Val Val Ser Ala Ser Val Asp Asn Ser Leu Trp Gly Lys         195 200 205 ggg ctt aac caa tct gaa ttg gat gat aat gat att gaa gag ctg atg 733 Gly Leu Asn Gln Ser Glu Leu Asp Asp Asn Asp Ile Glu Glu Leu Met     210 215 220 agt cag gtt aga gat cag tct ggt cca aca ttg cag cag aat ggg gtg 781 Ser Gln Val Arg Asp Gln Ser Gly Pro Thr Leu Gln Gln Asn Gly Val 225 230 235 240 tct gga ctg aac tct cat gta gac acg tat aat ctg gag aac ctg gag 829 Ser Gly Leu Asn Ser His Val Asp Thr Tyr Asn Leu Glu Asn Leu Glu                 245 250 255 gaa gat atg tat ttg gaa atc aat gat ctt atg gaa cct gaa cct gga 877 Glu Asp Met Tyr Leu Glu Ile Asn Asp Leu Met Glu Pro Glu Pro Gly             260 265 270 cca act tct gtg gaa gtc atg gag aat aac tgg aac gag gat ggt tct 925 Pro Thr Ser Val Glu Val Met Glu Asn Asn Trp Asn Glu Asp Gly Ser         275 280 285 ggt ctc ctg aat gat gat gat ttc gtt ggt gct gat tca tat ttc ctt 973 Gly Leu Leu Asn Asp Asp Asp Phe Val Gly Ala Asp Ser Tyr Phe Leu     290 295 300 gat ttg gga gtg aca aat cct cag tta gat ttt gtt agt ggt gat ttg 1021 Asp Leu Gly Val Thr Asn Pro Gln Leu Asp Phe Val Ser Gly Asp Leu 305 310 315 320 aaa aat ggg ttt gca caa agt ctt cag gtg aat act tct tta atg act 1069 Lys Asn Gly Phe Ala Gln Ser Leu Gln Val Asn Thr Ser Leu Met Thr                 325 330 335 tac cag gcc aat aat aac cag ttc cag cag caa tca ggg aag aac caa 1117 Tyr Gln Ala Asn Asn Asn Gln Phe Gln Gln Gln Ser Gly Lys Asn Gln             340 345 350 gct agt aac tgg cca ctc cgt aac agc tat acc aga cag ata aac aat 1165 Ala Ser Asn Trp Pro Leu Arg Asn Ser Tyr Thr Arg Gln Ile Asn Asn         355 360 365 gga tca tcg tgg gtg cag gag cta aac aat gac gga ctt acc gtt acc 1213 Gly Ser Ser Trp Val Gln Glu Leu Asn Asn Asp Gly Leu Thr Val Thr     370 375 380 cgg ttt ggt gag gcg cct ggt aca ggt gat tca tct gaa ttc cta aac 1261 Arg Phe Gly Glu Ala Pro Gly Thr Gly Asp Ser Ser Glu Phe Leu Asn 385 390 395 400 cct gtt cct tct ggt ata agt aca act aat gaa gat gac ccg tca aaa 1309 Pro Val Pro Ser Gly Ile Ser Thr Thr Asn Glu Asp Asp Pro Ser Lys                 405 410 415 gac gag tct agt aag ttt gct tct agt gta tgg act ttc ctg gaa tcc 1357 Asp Glu Ser Ser Lys Phe Ala Ser Ser Val Trp Thr Phe Leu Glu Ser             420 425 430 att cct gca aag cca gca tat gca tca gag aat cca ttt gtg aag ctg 1405 Ile Pro Ala Lys Pro Ala Tyr Ala Ser Glu Asn Pro Phe Val Lys Leu         435 440 445 aac ctt gtt aga atg tca acc agt ggt ggt cgt ttc agg ttt act tct 1453 Asn Leu Val Arg Met Ser Thr Ser Gly Gly Arg Phe Arg Phe Thr Ser     450 455 460 aaa agc aca ggt aat aat gtt gtt gtt atg gat agt gac tca gca gtg 1501 Lys Ser Thr Gly Asn Asn Val Val Val Met Asp Ser Asp Ser Ala Val 465 470 475 480 aag agg aac aag tct gga gga aac aac gat aag aag aag aag aag aac 1549 Lys Arg Asn Lys Ser Gly Gly Asn Asn Asp Lys Lys Lys Lys Lys Asn                 485 490 495 aaa ggt ttc ttt tgc tta tcg atc att ggg gct tta tgt gct ttg ttt 1597 Lys Gly Phe Phe Cys Leu Ser Ile Ile Gly Ala Leu Cys Ala Leu Phe             500 505 510 tgg gtg atc ata gga aca atg gga ggt tca ggg agg cct ttg tta tgg 1645 Trp Val Ile Ile Gly Thr Met Gly Gly Ser Gly Arg Pro Leu Leu Trp         515 520 525 tgagaaccga aaaatccaag aggttaagag acataaaggc ttggttttgt gtgaaccatt 1705 agagagtcaa gtcattgtaa ttattctctt ggattattag attcagaagc tgtttagtat 1765 cacagtttat gcttggaagt tttctctggt attgttaaaa aagtaccaat agaaataagc 1825 aaaagaattt tcttatcttt taggatattt gaacaaatga tgttacttaa ctagattatt 1885 aacttggagg ttgttgtaca aaaaaaaaaa aaaaa 1920 <210> 56 <211> 528 <212> PRT <213> Arabidopsis thaliana <400> 56 Met Asp Leu Ser Val Glu Asn Gly Gly Leu Ala Pro Gly Phe Arg Phe   1 5 10 15 His Pro Thr Asp Glu Glu Leu Val Val Tyr Tyr Leu Lys Arg Lys Ile              20 25 30 Arg Arg Lys Lys Leu Arg Val Glu Ala Ile Gly Glu Thr Asp Val Tyr          35 40 45 Lys Phe Asp Pro Glu Glu Leu Pro Glu Lys Ala Leu Tyr Lys Thr Arg      50 55 60 Asp Arg Gln Trp Phe Phe Phe Ser Leu Arg Asp Arg Lys His Gly Ser  65 70 75 80 Arg Ser Ser Arg Ala Thr Glu Arg Gly Tyr Trp Lys Ala Thr Gly Lys                  85 90 95 Asp Arg Val Ile His Cys Asp Ser Arg Pro Val Gly Glu Lys Lys Thr             100 105 110 Leu Val Phe His Arg Gly Arg Ala Pro Asn Gly Glu Arg Thr Asn Trp         115 120 125 Val Met His Glu Tyr Thr Leu His Lys Glu Glu Leu Lys Arg Cys Gly     130 135 140 Gly Glu Asp Val Lys Asp Ala Tyr Val Leu Tyr Lys Ile Tyr Lys Lys 145 150 155 160 Ser Gly Ser Gly Pro Lys Asn Gly Glu Gln Tyr Gly Ala Pro Phe Ile                 165 170 175 Glu Glu Glu Trp Ala Glu Asp Asp Asp Asp Asp Val Asp Glu Pro Ala             180 185 190 Asn Gln Leu Val Val Ser Ala Ser Val Asp Asn Ser Leu Trp Gly Lys         195 200 205 Gly Leu Asn Gln Ser Glu Leu Asp Asp Asn Asp Ile Glu Glu Leu Met     210 215 220 Ser Gln Val Arg Asp Gln Ser Gly Pro Thr Leu Gln Gln Asn Gly Val 225 230 235 240 Ser Gly Leu Asn Ser His Val Asp Thr Tyr Asn Leu Glu Asn Leu Glu                 245 250 255 Glu Asp Met Tyr Leu Glu Ile Asn Asp Leu Met Glu Pro Glu Pro Gly             260 265 270 Pro Thr Ser Val Glu Val Met Glu Asn Asn Trp Asn Glu Asp Gly Ser         275 280 285 Gly Leu Leu Asn Asp Asp Asp Phe Val Gly Ala Asp Ser Tyr Phe Leu     290 295 300 Asp Leu Gly Val Thr Asn Pro Gln Leu Asp Phe Val Ser Gly Asp Leu 305 310 315 320 Lys Asn Gly Phe Ala Gln Ser Leu Gln Val Asn Thr Ser Leu Met Thr                 325 330 335 Tyr Gln Ala Asn Asn Asn Gln Phe Gln Gln Gln Ser Gly Lys Asn Gln             340 345 350 Ala Ser Asn Trp Pro Leu Arg Asn Ser Tyr Thr Arg Gln Ile Asn Asn         355 360 365 Gly Ser Ser Trp Val Gln Glu Leu Asn Asn Asp Gly Leu Thr Val Thr     370 375 380 Arg Phe Gly Glu Ala Pro Gly Thr Gly Asp Ser Ser Glu Phe Leu Asn 385 390 395 400 Pro Val Pro Ser Gly Ile Ser Thr Thr Asn Glu Asp Asp Pro Ser Lys                 405 410 415 Asp Glu Ser Ser Lys Phe Ala Ser Ser Val Trp Thr Phe Leu Glu Ser             420 425 430 Ile Pro Ala Lys Pro Ala Tyr Ala Ser Glu Asn Pro Phe Val Lys Leu         435 440 445 Asn Leu Val Arg Met Ser Thr Ser Gly Gly Arg Phe Arg Phe Thr Ser     450 455 460 Lys Ser Thr Gly Asn Asn Val Val Val Met Asp Ser Asp Ser Ala Val 465 470 475 480 Lys Arg Asn Lys Ser Gly Gly Asn Asn Asp Lys Lys Lys Lys Lys Asn                 485 490 495 Lys Gly Phe Phe Cys Leu Ser Ile Ile Gly Ala Leu Cys Ala Leu Phe             500 505 510 Trp Val Ile Ile Gly Thr Met Gly Gly Ser Gly Arg Pro Leu Leu Trp         515 520 525 <210> 57 <211> 1219 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (177) .. (950) <400> 57 atgctaagga gccctcccaa aaaagaacaa caaatcacat ttttatataa ctgttaacat 60 aataatctca gcctcatcaa cacacatata tagatagcca acatcacaca aacatagaga 120 ttccaaaaaa taaaaataaa gaaaacataa atcctctgag gaaaaattcc gatgag atg 179                                                               Met                                                                 1 aca gaa ggt gga gaa tat tct ccg gcg atg atg tca gca gag cca ttc 227 Thr Glu Gly Gly Glu Tyr Ser Pro Ala Met Met Ser Ala Glu Pro Phe               5 10 15 ttg acc atg aag aag atg aag aag agc aac cac aac aag aac aat cag 275 Leu Thr Met Lys Lys Met Lys Lys Ser Asn His Asn Lys Asn Asn Gln          20 25 30 aga agg ttt agc gac gag cag atc aag tca ctg gag atg atg ttt gag 323 Arg Arg Phe Ser Asp Glu Gln Ile Lys Ser Leu Glu Met Met Phe Glu      35 40 45 tct gag aca agg ctt gag cca agg aag aag gtt caa tta gct aga gag 371 Ser Glu Thr Arg Leu Glu Pro Arg Lys Lys Val Gln Leu Ala Arg Glu  50 55 60 65 cta ggg ttg cag ccg agg caa gtg gct ata tgg ttt cag aac aag agg 419 Leu Gly Leu Gln Pro Arg Gln Val Ala Ile Trp Phe Gln Asn Lys Arg                  70 75 80 gct cgt tgg aaa tcc aag cag ctc gag act gag tac aac att ctc aga 467 Ala Arg Trp Lys Ser Lys Gln Leu Glu Thr Glu Tyr Asn Ile Leu Arg              85 90 95 caa aac tac gac aac ttg gct tct cag ttc gag tcc tta aag aaa gaa 515 Gln Asn Tyr Asp Asn Leu Ala Ser Gln Phe Glu Ser Leu Lys Lys Glu         100 105 110 aaa caa gct tta gtc tct gag ttg cag agg cta aaa gag gcg acg caa 563 Lys Gln Ala Leu Val Ser Glu Leu Gln Arg Leu Lys Glu Ala Thr Gln     115 120 125 aag aag aca cag gag gag gaa agg cag tgt agt gga gat caa gcg gtg 611 Lys Lys Thr Gln Glu Glu Glu Arg Gln Cys Ser Gly Asp Gln Ala Val 130 135 140 145 gtt gct cta agc agc aca cat cat gaa tca gaa aac gaa gag aac cgg 659 Val Ala Leu Ser Ser Thr His His Glu Ser Glu Asn Glu Glu Asn Arg                 150 155 160 agg cgt aaa ccg gaa gag gtt aga ccg gag atg gag atg aaa gat gat 707 Arg Arg Lys Pro Glu Glu Val Arg Pro Glu Met Glu Met Lys Asp Asp             165 170 175 aag ggt cat cat ggg gtt atg tgt gat cat cat gat tat gaa gat gat 755 Lys Gly His His Gly Val Met Cys Asp His His Asp Tyr Glu Asp Asp         180 185 190 gat aat ggt tat agt aac aac atc aag aga gag tat ttt ggt ggg ttt 803 Asp Asn Gly Tyr Ser Asn Asn Ile Lys Arg Glu Tyr Phe Gly Gly Phe     195 200 205 gag gaa gaa cca gat cac tta atg aac att gtt gaa cca gct gat agt 851 Glu Glu Glu Pro Asp His Leu Met Asn Ile Val Glu Pro Ala Asp Ser 210 215 220 225 tgt ttg aca tca tct gat gat tgg aga ggt ttc aaa tca gat act act 899 Cys Leu Thr Ser Ser Asp Asp Trp Arg Gly Phe Lys Ser Asp Thr Thr                 230 235 240 act ctc ttg gac caa tcc agc aac aat tac cct tgg cgg gat ttt tgg 947 Thr Leu Leu Asp Gln Ser Ser Asn Asn Tyr Pro Trp Arg Asp Phe Trp             245 250 255 tca tgaaaacaat aaactctaaa caagaagatg aaacagattg agactaaaga 1000 Ser ttggatatat acatattcaa atcgaaattt accggtctac atcgcatgaa ccgagccacg 1060 gatatagaga tattcggtcc agcaaatgac tcgtttctca gcgagaattt tgcaggattt 1120 tgagctgaaa ttgtatggtt ttgtctgtat aaatgatgtg tttagaaaga cgtatattct 1180 caaaaaaaaa aaaaaaaaaa aagaaaaaaa aaaaaaaaa 1219 <210> 58 <211> 258 <212> PRT <213> Arabidopsis thaliana <400> 58 Met Thr Glu Gly Gly Glu Tyr Ser Pro Ala Met Met Ser Ala Glu Pro   1 5 10 15 Phe Leu Thr Met Lys Lys Met Lys Lys Ser Asn His Asn Lys Asn Asn              20 25 30 Gln Arg Arg Phe Ser Asp Glu Gln Ile Lys Ser Leu Glu Met Met Phe          35 40 45 Glu Ser Glu Thr Arg Leu Glu Pro Arg Lys Lys Val Gln Leu Ala Arg      50 55 60 Glu Leu Gly Leu Gln Pro Arg Gln Val Ala Ile Trp Phe Gln Asn Lys  65 70 75 80 Arg Ala Arg Trp Lys Ser Lys Gln Leu Glu Thr Glu Tyr Asn Ile Leu                  85 90 95 Arg Gln Asn Tyr Asp Asn Leu Ala Ser Gln Phe Glu Ser Leu Lys Lys             100 105 110 Glu Lys Gln Ala Leu Val Ser Glu Leu Gln Arg Leu Lys Glu Ala Thr         115 120 125 Gln Lys Lys Thr Gln Glu Glu Glu Arg Gln Cys Ser Gly Asp Gln Ala     130 135 140 Val Val Ala Leu Ser Ser Thr His His Glu Ser Glu Asn Glu Glu Asn 145 150 155 160 Arg Arg Arg Lys Pro Glu Glu Val Arg Pro Glu Met Glu Met Lys Asp                 165 170 175 Asp Lys Gly His His Gly Val Met Cys Asp His His Asp Tyr Glu Asp             180 185 190 Asp Asp Asn Gly Tyr Ser Asn Asn Ile Lys Arg Glu Tyr Phe Gly Gly         195 200 205 Phe Glu Glu Glu Pro Asp His Leu Met Asn Ile Val Glu Pro Ala Asp     210 215 220 Ser Cys Leu Thr Ser Ser Asp Asp Trp Arg Gly Phe Lys Ser Asp Thr 225 230 235 240 Thr Thr Leu Leu Asp Gln Ser Ser Asn Asn Tyr Pro Trp Arg Asp Phe                 245 250 255 Trp Ser <210> 59 <211> 978 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (89) .. (793) <400> 59 aaagaaagaa agaaaaaaaa gaaacaaata attccaaaac cttctctctt aatcaaaatc 60 aagaaactta caagatctgg tgaaaacc atg gaa gaa gga gat ttt ttc aac 112                                Met Glu Glu Gly Asp Phe Phe Asn                                  1 5 tgc tgt ttc agc gag att agt agt ggc atg acc atg aat aag aag aag 160 Cys Cys Phe Ser Glu Ile Ser Ser Gly Met Thr Met Asn Lys Lys Lys      10 15 20 atg aag aag agc aat aac caa aag agg ttt agc gag gaa cag atc aag 208 Met Lys Lys Ser Asn Asn Gln Lys Arg Phe Ser Glu Glu Gln Ile Lys  25 30 35 40 tca ctt gag ctt ata ttt gag tct gag acg agg ctt gag ccg agg aag 256 Ser Leu Glu Leu Ile Phe Glu Ser Glu Thr Arg Leu Glu Pro Arg Lys                  45 50 55 aag gtt cag gta gct aga gag cta ggg ctg caa cca aga caa gtg gct 304 Lys Val Gln Val Ala Arg Glu Leu Gly Leu Gln Pro Arg Gln Val Ala              60 65 70 ata tgg ttt caa aac aag agg gct cga tgg aaa act aag caa ctt gag 352 Ile Trp Phe Gln Asn Lys Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu          75 80 85 aaa gag tat aac act ctt aga gcc aat tac aac aat ttg gct tca caa 400 Lys Glu Tyr Asn Thr Leu Arg Ala Asn Tyr Asn Asn Leu Ala Ser Gln      90 95 100 ttt gaa atc atg aag aaa gaa aag caa tct ctg gtc tct gag ctg cag 448 Phe Glu Ile Met Lys Lys Glu Lys Gln Ser Leu Val Ser Glu Leu Gln 105 110 115 120 aga cta aac gaa gag atg caa agg cct aaa gaa gaa aag cat cat gag 496 Arg Leu Asn Glu Glu Met Gln Arg Pro Lys Glu Glu Lys His His Glu                 125 130 135 tgt tgt ggt gat caa gga ctg gct cta agc agc agc aca gag tcg cat 544 Cys Cys Gly Asp Gln Gly Leu Ala Leu Ser Ser Ser Thr Glu Ser His             140 145 150 aat gga aag agt gag cca gaa ggg agg tta gac caa ggg agt gtt cta 592 Asn Gly Lys Ser Glu Pro Glu Gly Arg Leu Asp Gln Gly Ser Val Leu         155 160 165 tgt aat gat ggt gat tac aac aac aac att aaa aca gag tat ttt ggg 640 Cys Asn Asp Gly Asp Tyr Asn Asn Asn Ile Lys Thr Glu Tyr Phe Gly     170 175 180 ttc gag gaa gag act gat cat gag ctg atg aac att gtg gag aaa gct 688 Phe Glu Glu Glu Thr Asp His Glu Leu Met Asn Ile Val Glu Lys Ala 185 190 195 200 gat gat agt tgc ttg aca tct tct gag aat tgg gga ggt ttc aat tct 736 Asp Asp Ser Cys Leu Thr Ser Ser Glu Asn Trp Gly Gly Phe Asn Ser                 205 210 215 gat tct ctc tta gac caa tct agc agc aat tac cct aac tgg tgg gag 784 Asp Ser Leu Leu Asp Gln Ser Ser Ser Asn Tyr Pro Asn Trp Trp Glu             220 225 230 ttt tgg tca taaaagcata taagaaaaaa acagaacata agcgaagaga 833 Phe Trp Ser         235 aagagtgtga atagtttgta aattatgtgt taagaaaaat aaatttagtt tagtttaaat 893 cttgtttcga tctatgtatc tactatgttc aatactcttt gtagctaatt agtagcttat 953 aatgagacta gaaaagtttt gaagc 978 <210> 60 <211> 235 <212> PRT <213> Arabidopsis thaliana <400> 60 Met Glu Glu Gly Asp Phe Phe Asn Cys Cys Phe Ser Glu Ile Ser Ser   1 5 10 15 Gly Met Thr Met Asn Lys Lys Lys Met Lys Lys Ser Asn Asn Gln Lys              20 25 30 Arg Phe Ser Glu Glu Gln Ile Lys Ser Leu Glu Leu Ile Phe Glu Ser          35 40 45 Glu Thr Arg Leu Glu Pro Arg Lys Lys Val Gln Val Ala Arg Glu Leu      50 55 60 Gly Leu Gln Pro Arg Gln Val Ala Ile Trp Phe Gln Asn Lys Arg Ala  65 70 75 80 Arg Trp Lys Thr Lys Gln Leu Glu Lys Glu Tyr Asn Thr Leu Arg Ala                  85 90 95 Asn Tyr Asn Asn Leu Ala Ser Gln Phe Glu Ile Met Lys Lys Glu Lys             100 105 110 Gln Ser Leu Val Ser Glu Leu Gln Arg Leu Asn Glu Glu Met Gln Arg         115 120 125 Pro Lys Glu Glu Lys His His Glu Cys Cys Gly Asp Gln Gly Leu Ala     130 135 140 Leu Ser Ser Ser Thr Glu Ser His Asn Gly Lys Ser Glu Pro Glu Gly 145 150 155 160 Arg Leu Asp Gln Gly Ser Val Leu Cys Asn Asp Gly Asp Tyr Asn Asn                 165 170 175 Asn Ile Lys Thr Glu Tyr Phe Gly Phe Glu Glu Glu Thr Asp His Glu             180 185 190 Leu Met Asn Ile Val Glu Lys Ala Asp Asp Ser Cys Leu Thr Ser Ser         195 200 205 Glu Asn Trp Gly Gly Phe Asn Ser Asp Ser Leu Leu Asp Gln Ser Ser     210 215 220 Ser Asn Tyr Pro Asn Trp Trp Glu Phe Trp Ser 225 230 235 <210> 61 <211> 2043 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (2040) <400> 61 atg gct gct tac ttt cac gga aac cca ccg gag atc tct gcc gga tcc 48 Met Ala Ala Tyr Phe His Gly Asn Pro Pro Glu Ile Ser Ala Gly Ser   1 5 10 15 gac ggt ggt ctt caa acg ttg atc ctc atg aat cca act act tac gtt 96 Asp Gly Gly Leu Gln Thr Leu Ile Leu Met Asn Pro Thr Thr Tyr Val              20 25 30 cag tac acc caa caa gac aac gac tcg aac aac aac aac aac agc aac 144 Gln Tyr Thr Gln Gln Asp Asn Asp Ser Asn Asn Asn Asn Asn Ser Asn          35 40 45 aat agc aac aac aac aac aca aac aca aac aca aac aac aac aac agt 192 Asn Ser Asn Asn Asn Asn Thr Asn Thr Asn Thr Asn Asn Asn Asn Ser      50 55 60 agt ttc gtt ttc ctc gat tcc cac gcg ccg cag cca aac gcg agc cag 240 Ser Phe Val Phe Leu Asp Ser His Ala Pro Gln Pro Asn Ala Ser Gln  65 70 75 80 cag ttc gtc gga ata cca ctc tca ggt cac gaa gct gct tcc att aca 288 Gln Phe Val Gly Ile Pro Leu Ser Gly His Glu Ala Ala Ser Ile Thr                  85 90 95 gcc gcc gac aac atc tcc gta ctt cac ggt tat cct ccg cgc gtg cag 336 Ala Ala Asp Asn Ile Ser Val Leu His Gly Tyr Pro Pro Arg Val Gln             100 105 110 tac agt ctc tac ggt agc cac caa gtg gat ccc act cac cag caa gcc 384 Tyr Ser Leu Tyr Gly Ser His Gln Val Asp Pro Thr His Gln Gln Ala         115 120 125 gcg tgt gag acg cca cgc gcg cag caa ggc ctc tct tta acc ctc tcg 432 Ala Cys Glu Thr Pro Arg Ala Gln Gln Gly Leu Ser Leu Thr Leu Ser     130 135 140 tct caa cag cag cag caa cag caa cat cat caa caa cac cag cct att 480 Ser Gln Gln Gln Gln Gln Gln Gln His His Gln Gln His Gln Pro Ile 145 150 155 160 cac gtc gga ttc ggg tcc gga cat gga gaa gat atc cgg gtc ggg tct 528 His Val Gly Phe Gly Ser Gly His Gly Glu Asp Ile Arg Val Gly Ser                 165 170 175 ggc tct aca gga tcg ggg gta aca aac ggt ata gct aat ctt gtt agc 576 Gly Ser Thr Gly Ser Gly Val Thr Asn Gly Ile Ala Asn Leu Val Ser             180 185 190 tcc aag tac ttg aag gca gca caa gag ctt ctt gac gaa gta gtc aac 624 Ser Lys Tyr Leu Lys Ala Ala Gln Glu Leu Leu Asp Glu Val Val Asn         195 200 205 gct gat tcc gat gac atg aac gct aaa tcc caa cta ttc tca tcg aaa 672 Ala Asp Ser Asp Asp Met Asn Ala Lys Ser Gln Leu Phe Ser Ser Lys     210 215 220 aag ggt agt tgc gga aat gat aaa cct gtc gga gaa tca tcg gcc ggc 720 Lys Gly Ser Cys Gly Asn Asp Lys Pro Val Gly Glu Ser Ser Ala Gly 225 230 235 240 gct gga gga gaa ggt tcc ggt ggc gga gca gaa gca gcc ggg aaa cgt 768 Ala Gly Gly Glu Gly Ser Gly Gly Gly Ala Glu Ala Ala Gly Lys Arg                 245 250 255 ccg gtg gag cta ggc acg gca gag aga caa gaa ata cag atg aag aaa 816 Pro Val Glu Leu Gly Thr Ala Glu Arg Gln Glu Ile Gln Met Lys Lys             260 265 270 gca aaa ctt agt aac atg ctt cat gag gtg gag cag aga tat aga cag 864 Ala Lys Leu Ser Asn Met Leu His Glu Val Glu Gln Arg Tyr Arg Gln         275 280 285 tac cac cag cag atg cag atg gtg atc tct tcg ttc gag caa gcg gca 912 Tyr His Gln Gln Met Gln Met Val Ile Ser Ser Phe Glu Gln Ala Ala     290 295 300 ggg ata gga tca gcg aag tca tac acg tcg cta gca ttg aaa acc ata 960 Gly Ile Gly Ser Ala Lys Ser Tyr Thr Ser Leu Ala Leu Lys Thr Ile 305 310 315 320 tca aga cag ttc cgt tgc ttg aaa gag gcg atc gct ggt cag ata aaa 1008 Ser Arg Gln Phe Arg Cys Leu Lys Glu Ala Ile Ala Gly Gln Ile Lys                 325 330 335 gcg gcc aac aag agt ctt ggg gag gaa gat tca gtg tct ggt gtt ggg 1056 Ala Ala Asn Lys Ser Leu Gly Glu Glu Asp Ser Val Ser Gly Val Gly             340 345 350 agg ttt gag ggg tcg agg ctc aag ttc gtg gac cac cac ttg aga cag 1104 Arg Phe Glu Gly Ser Arg Leu Lys Phe Val Asp His His Leu Arg Gln         355 360 365 caa aga gct ctt caa caa ctg gga atg att caa cat cct tcc aat aat 1152 Gln Arg Ala Leu Gln Gln Leu Gly Met Ile Gln His Pro Ser Asn Asn     370 375 380 gct tgg aga cct caa cgt ggt ctc cca gaa cga gcc gtc tca gtt ctc 1200 Ala Trp Arg Pro Gln Arg Gly Leu Pro Glu Arg Ala Val Ser Val Leu 385 390 395 400 cgt gct tgg ctc ttc gaa cac ttt ctt cat cca tac cct aag gat tcg 1248 Arg Ala Trp Leu Phe Glu His Phe Leu His Pro Tyr Pro Lys Asp Ser                 405 410 415 gac aag cac atg cta gct aag caa aca gga ctc act cgt agc cag gtg 1296 Asp Lys His Met Leu Ala Lys Gln Thr Gly Leu Thr Arg Ser Gln Val             420 425 430 tcg aac tgg ttt ata aac gcg aga gtt cgg tta tgg aaa cca atg gtg 1344 Ser Asn Trp Phe Ile Asn Ala Arg Val Arg Leu Trp Lys Pro Met Val         435 440 445 gag gag atg tac atg gag gaa atg aag gag cag gca aag aac atg gga 1392 Glu Glu Met Tyr Met Glu Glu Met Lys Glu Gln Ala Lys Asn Met Gly     450 455 460 tcc atg gaa aag act cct ttg gat caa agc aac gaa gat tct gct tca 1440 Ser Met Glu Lys Thr Pro Leu Asp Gln Ser Asn Glu Asp Ser Ala Ser 465 470 475 480 aag tca aca agt aac caa gaa aag agc cca atg gcg gac act aat tac 1488 Lys Ser Thr Ser Asn Gln Glu Lys Ser Pro Met Ala Asp Thr Asn Tyr                 485 490 495 cat atg aat ccc aat cac aac ggt gac cta gaa ggc gtc act gga atg 1536 His Met Asn Pro Asn His Asn Gly Asp Leu Glu Gly Val Thr Gly Met             500 505 510 caa gga agc ccc aag aga cta aga acc agc gac gag aca atg atg cag 1584 Gln Gly Ser Pro Lys Arg Leu Arg Thr Ser Asp Glu Thr Met Met Gln         515 520 525 cca ata aat gcg gat ttc agc tcc aac gag aag ctc acg atg aag att 1632 Pro Ile Asn Ala Asp Phe Ser Ser Asn Glu Lys Leu Thr Met Lys Ile     530 535 540 cta gaa gaa cgg caa ggg ata aga tca gat ggt ggc tac cct ttc atg 1680 Leu Glu Glu Arg Gln Gly Ile Arg Ser Asp Gly Gly Tyr Pro Phe Met 545 550 555 560 ggt aat ttc ggg caa tac caa atg gat gag atg tca aga ttt gat gta 1728 Gly Asn Phe Gly Gln Tyr Gln Met Asp Glu Met Ser Arg Phe Asp Val                 565 570 575 gtc tca gac cag gag ctc atg gcg caa agg tac tca gga aac aac aat 1776 Val Ser Asp Gln Glu Leu Met Ala Gln Arg Tyr Ser Gly Asn Asn Asn             580 585 590 ggc gtg tcc ctc acg tta ggt tta cct cat tgt gat agc ttg tcg tcc 1824 Gly Val Ser Leu Thr Leu Gly Leu Pro His Cys Asp Ser Leu Ser Ser         595 600 605 acg cac cat cag ggt ttc atg cag acc cac cat ggg att cct ata ggg 1872 Thr His His Gln Gly Phe Met Gln Thr His His Gly Ile Pro Ile Gly     610 615 620 aga aga gtg aaa ata gga gaa aca gag gaa tat gga ccc gcc acc atc 1920 Arg Arg Val Lys Ile Gly Glu Thr Glu Glu Tyr Gly Pro Ala Thr Ile 625 630 635 640 aat ggt ggt agc tcg acc aca acc gca cat tca tca gcg gca gct gcc 1968 Asn Gly Gly Ser Ser Thr Thr Thr Ala His Ser Ser Ala Ala Ala Ala                 645 650 655 gcg gct tac aat ggg atg aac ata cag aac cag aag aga tat gtg gct 2016 Ala Ala Tyr Asn Gly Met Asn Ile Gln Asn Gln Lys Arg Tyr Val Ala             660 665 670 cag tta ttg ccc gac ttc gtt gca taa 2043 Gln Leu Leu Pro Asp Phe Val Ala         675 680 <210> 62 <211> 680 <212> PRT <213> Arabidopsis thaliana <400> 62 Met Ala Ala Tyr Phe His Gly Asn Pro Pro Glu Ile Ser Ala Gly Ser   1 5 10 15 Asp Gly Gly Leu Gln Thr Leu Ile Leu Met Asn Pro Thr Thr Tyr Val              20 25 30 Gln Tyr Thr Gln Gln Asp Asn Asp Ser Asn Asn Asn Asn Asn Ser Asn          35 40 45 Asn Ser Asn Asn Asn Asn Thr Asn Thr Asn Thr Asn Asn Asn Asn Ser      50 55 60 Ser Phe Val Phe Leu Asp Ser His Ala Pro Gln Pro Asn Ala Ser Gln  65 70 75 80 Gln Phe Val Gly Ile Pro Leu Ser Gly His Glu Ala Ala Ser Ile Thr                  85 90 95 Ala Ala Asp Asn Ile Ser Val Leu His Gly Tyr Pro Pro Arg Val Gln             100 105 110 Tyr Ser Leu Tyr Gly Ser His Gln Val Asp Pro Thr His Gln Gln Ala         115 120 125 Ala Cys Glu Thr Pro Arg Ala Gln Gln Gly Leu Ser Leu Thr Leu Ser     130 135 140 Ser Gln Gln Gln Gln Gln Gln Gln His His Gln Gln His Gln Pro Ile 145 150 155 160 His Val Gly Phe Gly Ser Gly His Gly Glu Asp Ile Arg Val Gly Ser                 165 170 175 Gly Ser Thr Gly Ser Gly Val Thr Asn Gly Ile Ala Asn Leu Val Ser             180 185 190 Ser Lys Tyr Leu Lys Ala Ala Gln Glu Leu Leu Asp Glu Val Val Asn         195 200 205 Ala Asp Ser Asp Asp Met Asn Ala Lys Ser Gln Leu Phe Ser Ser Lys     210 215 220 Lys Gly Ser Cys Gly Asn Asp Lys Pro Val Gly Glu Ser Ser Ala Gly 225 230 235 240 Ala Gly Gly Glu Gly Ser Gly Gly Gly Ala Glu Ala Ala Gly Lys Arg                 245 250 255 Pro Val Glu Leu Gly Thr Ala Glu Arg Gln Glu Ile Gln Met Lys Lys             260 265 270 Ala Lys Leu Ser Asn Met Leu His Glu Val Glu Gln Arg Tyr Arg Gln         275 280 285 Tyr His Gln Gln Met Gln Met Val Ile Ser Ser Phe Glu Gln Ala Ala     290 295 300 Gly Ile Gly Ser Ala Lys Ser Tyr Thr Ser Leu Ala Leu Lys Thr Ile 305 310 315 320 Ser Arg Gln Phe Arg Cys Leu Lys Glu Ala Ile Ala Gly Gln Ile Lys                 325 330 335 Ala Ala Asn Lys Ser Leu Gly Glu Glu Asp Ser Val Ser Gly Val Gly             340 345 350 Arg Phe Glu Gly Ser Arg Leu Lys Phe Val Asp His His Leu Arg Gln         355 360 365 Gln Arg Ala Leu Gln Gln Leu Gly Met Ile Gln His Pro Ser Asn Asn     370 375 380 Ala Trp Arg Pro Gln Arg Gly Leu Pro Glu Arg Ala Val Ser Val Leu 385 390 395 400 Arg Ala Trp Leu Phe Glu His Phe Leu His Pro Tyr Pro Lys Asp Ser                 405 410 415 Asp Lys His Met Leu Ala Lys Gln Thr Gly Leu Thr Arg Ser Gln Val             420 425 430 Ser Asn Trp Phe Ile Asn Ala Arg Val Arg Leu Trp Lys Pro Met Val         435 440 445 Glu Glu Met Tyr Met Glu Glu Met Lys Glu Gln Ala Lys Asn Met Gly     450 455 460 Ser Met Glu Lys Thr Pro Leu Asp Gln Ser Asn Glu Asp Ser Ala Ser 465 470 475 480 Lys Ser Thr Ser Asn Gln Glu Lys Ser Pro Met Ala Asp Thr Asn Tyr                 485 490 495 His Met Asn Pro Asn His Asn Gly Asp Leu Glu Gly Val Thr Gly Met             500 505 510 Gln Gly Ser Pro Lys Arg Leu Arg Thr Ser Asp Glu Thr Met Met Gln         515 520 525 Pro Ile Asn Ala Asp Phe Ser Ser Asn Glu Lys Leu Thr Met Lys Ile     530 535 540 Leu Glu Glu Arg Gln Gly Ile Arg Ser Asp Gly Gly Tyr Pro Phe Met 545 550 555 560 Gly Asn Phe Gly Gln Tyr Gln Met Asp Glu Met Ser Arg Phe Asp Val                 565 570 575 Val Ser Asp Gln Glu Leu Met Ala Gln Arg Tyr Ser Gly Asn Asn Asn             580 585 590 Gly Val Ser Leu Thr Leu Gly Leu Pro His Cys Asp Ser Leu Ser Ser         595 600 605 Thr His His Gln Gly Phe Met Gln Thr His His Gly Ile Pro Ile Gly     610 615 620 Arg Arg Val Lys Ile Gly Glu Thr Glu Glu Tyr Gly Pro Ala Thr Ile 625 630 635 640 Asn Gly Gly Ser Ser Thr Thr Thr Ala His Ser Ser Ala Ala Ala Ala                 645 650 655 Ala Ala Tyr Asn Gly Met Asn Ile Gln Asn Gln Lys Arg Tyr Val Ala             660 665 670 Gln Leu Leu Pro Asp Phe Val Ala         675 680 <210> 63 <211> 1162 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (49) .. (933) <400> 63 aaattcgctt ttttttttct tctttgtata tttttttttt ttttgacc atg gcg gag 57                                                      Met Ala Glu                                                        1 gaa ttt gga agc ata gat tta ctc gga gat gaa gat ttc ttc ttc gat 105 Glu Phe Gly Ser Ile Asp Leu Leu Gly Asp Glu Asp Phe Phe Phe Asp       5 10 15 ttc gat cct tca atc gta att gat tct ctt ccg gcg gag gat ttt ctt 153 Phe Asp Pro Ser Ile Val Ile Asp Ser Leu Pro Ala Glu Asp Phe Leu  20 25 30 35 cag tct tca ccg gat tca tgg atc gga gaa atc gag aat caa ttg atg 201 Gln Ser Ser Pro Asp Ser Trp Ile Gly Glu Ile Glu Asn Gln Leu Met                  40 45 50 aac gat gag aat cat caa gag gag agt ttt gtg gaa ttg gat cag caa 249 Asn Asp Glu Asn His Gln Glu Glu Ser Phe Val Glu Leu Asp Gln Gln              55 60 65 tcg gtt tca gat ttc ata gcg gat cta ctc gtt gat tat cca act agc 297 Ser Val Ser Asp Phe Ile Ala Asp Leu Leu Val Asp Tyr Pro Thr Ser          70 75 80 gat tct ggc tcc gtt gat ttg gcg gct gat aaa gtt cta acc gtc gat 345 Asp Ser Gly Ser Val Asp Leu Ala Ala Asp Lys Val Leu Thr Val Asp      85 90 95 tct ccc gcc gcc gct gat gat tcc ggg aag gag aat tcg gat ttg gtt 393 Ser Pro Ala Ala Ala Asp Asp Ser Gly Lys Glu Asn Ser Asp Leu Val 100 105 110 115 gtt gag aag aag tct aat gat tct ggt agc gag att cat gat gat gat 441 Val Glu Lys Lys Ser Asn Asp Ser Gly Ser Glu Ile His Asp Asp Asp                 120 125 130 gac gaa gaa gga gac gat gat gct gtg gct aaa aaa cga aga agg aga 489 Asp Glu Glu Gly Asp Asp Asp Asp Ala Val Ala Lys Lys Arg Arg Arg Arg             135 140 145 gta aga aat aga gat gcg gcg gtt aga tcg aga gag agg aag aag gaa 537 Val Arg Asn Arg Asp Ala Ala Val Arg Ser Arg Glu Arg Lys Lys Glu         150 155 160 tat gta caa gat tta gag aag aag agt aag tat ctc gaa aga gaa tgc 585 Tyr Val Gln Asp Leu Glu Lys Lys Ser Lys Tyr Leu Glu Arg Glu Cys     165 170 175 ttg aga cta gga cgt atg ctt gag tgc ttc gtt gct gaa aac cag tct 633 Leu Arg Leu Gly Arg Met Leu Glu Cys Phe Val Ala Glu Asn Gln Ser 180 185 190 195 cta cgt tac tgt ttg caa aag ggt aat ggc aat aat act acc atg atg 681 Leu Arg Tyr Cys Leu Gln Lys Gly Asn Gly Asn Asn Thr Thr Met Met                 200 205 210 tcg aag cag gag tct gct gtg ctc ttg ttg gaa tcc ctg ctg ttg ggt 729 Ser Lys Gln Glu Ser Ala Val Leu Leu Leu Glu Ser Leu Leu Leu Gly             215 220 225 tcc ctg ctt tgg ctt ctg gga gta aac ttc att tgc cta ttc cct tat 777 Ser Leu Leu Trp Leu Leu Gly Val Asn Phe Ile Cys Leu Phe Pro Tyr         230 235 240 atg tcc cac aca aag tgt tgc ctc cta cgt cca gaa cca gaa aag ctg 825 Met Ser His Thr Lys Cys Cys Leu Leu Arg Pro Glu Pro Glu Lys Leu     245 250 255 gtt cta aac ggg ctc ggg agt agt agc aaa ccg tct tat acc ggc gtt 873 Val Leu Asn Gly Leu Gly Ser Ser Ser Lys Pro Ser Tyr Thr Gly Val 260 265 270 275 agt cgg aga tgt aag ggt tcg agg cct agg atg aaa tac caa atc tta 921 Ser Arg Arg Cys Lys Gly Ser Arg Pro Arg Met Lys Tyr Gln Ile Leu                 280 285 290 acc ctt gcg gcg tgacaacgcc ttttttaact gcttcttttg cgcattttga 973 Thr Leu Ala Ala             295 gttgtagatg agtgtctttt agttttctct ctcttgtttt gtatttcgct gttgaaagtt 1033 ttctgtctaa tatcgataag ttaacagtga atgtgggtct tatggttatg gatgatatct 1093 atctaataat gcttctgcct ttaaaatgtt gattttgagg cataacttca ggcaaaaaaa 1153 aaaaagaaa 1162 <210> 64 <211> 295 <212> PRT <213> Arabidopsis thaliana <400> 64 Met Ala Glu Glu Phe Gly Ser Ile Asp Leu Leu Gly Asp Glu Asp Phe   1 5 10 15 Phe Phe Asp Phe Asp Pro Ser Ile Val Ile Asp Ser Leu Pro Ala Glu              20 25 30 Asp Phe Leu Gln Ser Ser Pro Asp Ser Trp Ile Gly Glu Ile Glu Asn          35 40 45 Gln Leu Met Asn Asp Glu Asn His Gln Glu Glu Ser Phe Val Glu Leu      50 55 60 Asp Gln Gln Ser Val Ser Asp Phe Ile Ala Asp Leu Leu Val Asp Tyr  65 70 75 80 Pro Thr Ser Asp Ser Gly Ser Val Asp Leu Ala Ala Asp Lys Val Leu                  85 90 95 Thr Val Asp Ser Pro Ala Ala Ala Asp Asp Ser Gly Lys Glu Asn Ser             100 105 110 Asp Leu Val Val Glu Lys Lys Ser Asn Asp Ser Gly Ser Glu Ile His         115 120 125 Asp Asp Asp Asp Glu Glu Gly Asp Asp Asp Ala Val Ala Lys Lys Arg     130 135 140 Arg Arg Arg Val Arg Asn Arg Asp Ala Ala Val Arg Ser Arg Glu Arg 145 150 155 160 Lys Lys Glu Tyr Val Gln Asp Leu Glu Lys Lys Ser Lys Tyr Leu Glu                 165 170 175 Arg Glu Cys Leu Arg Leu Gly Arg Met Leu Glu Cys Phe Val Ala Glu             180 185 190 Asn Gln Ser Leu Arg Tyr Cys Leu Gln Lys Gly Asn Gly Asn Asn Thr         195 200 205 Thr Met Met Ser Lys Gln Glu Ser Ala Val Leu Leu Leu Glu Ser Leu     210 215 220 Leu Leu Gly Ser Leu Leu Trp Leu Leu Gly Val Asn Phe Ile Cys Leu 225 230 235 240 Phe Pro Tyr Met Ser His Thr Lys Cys Cys Leu Leu Arg Pro Glu Pro                 245 250 255 Glu Lys Leu Val Leu Asn Gly Leu Gly Ser Ser Ser Lys Pro Ser Tyr             260 265 270 Thr Gly Val Ser Arg Arg Cys Lys Gly Ser Arg Pro Arg Met Lys Tyr         275 280 285 Gln Ile Leu Thr Leu Ala Ala     290 295 <210> 65 <211> 438 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (1) .. (435) <400> 65 atg gca aac gca gag aag aca agt tca ggt tcc gac ata gat gag aag 48 Met Ala Asn Ala Glu Lys Thr Ser Ser Gly Ser Asp Ile Asp Glu Lys   1 5 10 15 aaa aga aaa cgc aag tta tca aac cgc gaa tct gca agg agg tcg cgt 96 Lys Arg Lys Arg Lys Leu Ser Asn Arg Glu Ser Ala Arg Arg Ser Arg              20 25 30 ttg aag aaa cag aag tta atg gaa gac acg att cat gag atc tcc agt 144 Leu Lys Lys Gln Lys Leu Met Glu Asp Thr Ile His Glu Ile Ser Ser          35 40 45 ctt gaa cga cga atc aaa gag aac agt gag aga tgt cga gct gta aaa 192 Leu Glu Arg Arg Ile Lys Glu Asn Ser Glu Arg Cys Arg Ala Val Lys      50 55 60 cag agg ctt gac tcg gtc gaa acg gag aac gcg ggt ctt aga tcg gag 240 Gln Arg Leu Asp Ser Val Glu Thr Glu Asn Ala Gly Leu Arg Ser Glu  65 70 75 80 aag att tgg ctc tcg agt tac gtt agc gat tta gag aat atg att gct 288 Lys Ile Trp Leu Ser Ser Tyr Val Ser Asp Leu Glu Asn Met Ile Ala                  85 90 95 acg acg agt tta acg ctg acg cag agt ggt ggt ggc gat tgt gtc gac 336 Thr Thr Ser Leu Thr Leu Thr Gln Ser Gly Gly Gly Asp Cys Val Asp             100 105 110 gat cag aac gca aac gcg gga ata gcg gtt gga gat tgt aga cgt aca 384 Asp Gln Asn Ala Asn Ala Gly Ile Ala Val Gly Asp Cys Arg Arg Thr         115 120 125 ccg tgg aaa ttg agt tgt ggt tct cta caa cca atg gcg tcc ttt aag 432 Pro Trp Lys Leu Ser Cys Gly Ser Leu Gln Pro Met Ala Ser Phe Lys     130 135 140 aca tga 438 Thr 145 <210> 66 <211> 145 <212> PRT <213> Arabidopsis thaliana <400> 66 Met Ala Asn Ala Glu Lys Thr Ser Ser Gly Ser Asp Ile Asp Glu Lys   1 5 10 15 Lys Arg Lys Arg Lys Leu Ser Asn Arg Glu Ser Ala Arg Arg Ser Arg              20 25 30 Leu Lys Lys Gln Lys Leu Met Glu Asp Thr Ile His Glu Ile Ser Ser          35 40 45 Leu Glu Arg Arg Ile Lys Glu Asn Ser Glu Arg Cys Arg Ala Val Lys      50 55 60 Gln Arg Leu Asp Ser Val Glu Thr Glu Asn Ala Gly Leu Arg Ser Glu  65 70 75 80 Lys Ile Trp Leu Ser Ser Tyr Val Ser Asp Leu Glu Asn Met Ile Ala                  85 90 95 Thr Thr Ser Leu Thr Leu Thr Gln Ser Gly Gly Gly Asp Cys Val Asp             100 105 110 Asp Gln Asn Ala Asn Ala Gly Ile Ala Val Gly Asp Cys Arg Arg Thr         115 120 125 Pro Trp Lys Leu Ser Cys Gly Ser Leu Gln Pro Met Ala Ser Phe Lys     130 135 140 Thr 145 <210> 67 <211> 1697 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (74) .. (1435) <400> 67 ccaaagaaaa aaaataaatt cgaaggtaaa tatccagaag cttgatcctc ctagttgtac 60 gaaagcttga gta atg ggg tct aga tta aac ttc aag agc ttt gtt gat 109                Met Gly Ser Arg Leu Asn Phe Lys Ser Phe Val Asp                  1 5 10 ggt gtg agt gag cag cag cca acg gtg ggg act agt ctt cca ttg act 157 Gly Val Ser Glu Gln Gln Pro Thr Val Gly Thr Ser Leu Pro Leu Thr          15 20 25 agg cag aac tct gtg ttc tcg tta acc ttt gat gag ttt cag aac tca 205 Arg Gln Asn Ser Val Phe Ser Leu Thr Phe Asp Glu Phe Gln Asn Ser      30 35 40 tgg ggt ggt gga att ggg aaa gat ttt ggg tct atg aac atg gat gag 253 Trp Gly Gly Gly Ile Gly Lys Asp Phe Gly Ser Met Asn Met Asp Glu  45 50 55 60 ctc ttg aag aac att tgg act gca gag gaa agt cat tca atg atg gga 301 Leu Leu Lys Asn Ile Trp Thr Ala Glu Glu Ser His Ser Met Met Gly                  65 70 75 aac aat acc agt tac acc aac atc agc aat ggt aat agt gga aac act 349 Asn Asn Thr Ser Tyr Thr Asn Ile Ser Asn Gly Asn Ser Gly Asn Thr              80 85 90 gtt att aac ggc ggt ggt aac aac att ggt ggg tta gct gtt ggt gtg 397 Val Ile Asn Gly Gly Gly Asn Asn Ile Gly Gly Leu Ala Val Gly Val          95 100 105 gga gga gaa agt ggt ggt ttt ttc act ggt ggg agt ttg cag aga caa 445 Gly Gly Glu Ser Gly Gly Phe Phe Thr Gly Gly Ser Leu Gln Arg Gln     110 115 120 ggt tca ctt acc ttg cct cgg acg att agt cag aaa agg gtt gat gat 493 Gly Ser Leu Thr Leu Pro Arg Thr Ile Ser Gln Lys Arg Val Asp Asp 125 130 135 140 gtc tgg aag gag ctg atg aag gag gat gac att gga aat ggt gtt gtt 541 Val Trp Lys Glu Leu Met Lys Glu Asp Asp Ile Gly Asn Gly Val Val                 145 150 155 aat ggt ggg aca agc gga att ccg cag agg caa caa acg ctg gga gag 589 Asn Gly Gly Thr Ser Gly Ile Pro Gln Arg Gln Gln Thr Leu Gly Glu             160 165 170 atg act ttg gag gag ttt ttg gtc agg gct ggt gtg gtt agg gaa gaa 637 Met Thr Leu Glu Glu Phe Leu Val Arg Ala Gly Val Val Arg Glu Glu         175 180 185 cct caa ccg gtg gag agt gta act aac ttc aat ggc gga ttc tat gga 685 Pro Gln Pro Val Glu Ser Val Thr Asn Phe Asn Gly Gly Phe Tyr Gly     190 195 200 ttt ggc agt aat gga ggt ctt ggg aca gct agt aat ggg ttt gtt gca 733 Phe Gly Ser Asn Gly Gly Leu Gly Thr Ala Ser Asn Gly Phe Val Ala 205 210 215 220 aac caa cct caa gat ttg tca gga aat gga gta gcg gtg aga cag gat 781 Asn Gln Pro Gln Asp Leu Ser Gly Asn Gly Val Ala Val Arg Gln Asp                 225 230 235 ctg ctg act gct caa act cag cca cta cag atg cag cag cca cag atg 829 Leu Leu Thr Ala Gln Thr Gln Pro Leu Gln Met Gln Gln Pro Gln Met             240 245 250 gtg cag cag cca cag atg gtg cag cag ccg caa caa ctg ata cag acg 877 Val Gln Gln Pro Gln Met Val Gln Gln Pro Gln Gln Leu Ile Gln Thr         255 260 265 cag gag agg cct ttt ccc aaa cag acc act ata gca ttt tcc aac act 925 Gln Glu Arg Pro Phe Pro Lys Gln Thr Thr Ile Ala Phe Ser Asn Thr     270 275 280 gtt gat gtg gtt aac cgt tct caa cct gca aca cag tgc cag gaa gtg 973 Val Asp Val Val Asn Arg Ser Gln Pro Ala Thr Gln Cys Gln Glu Val 285 290 295 300 aag cct tca ata ctt gga att cat aac cat cct atg aac aac aat cta 1021 Lys Pro Ser Ile Leu Gly Ile His Asn His Pro Met Asn Asn Asn Leu                 305 310 315 ctg caa gct gtc gat ttt aaa aca gga gta acg gtt gca gca gta tct 1069 Leu Gln Ala Val Asp Phe Lys Thr Gly Val Thr Val Ala Ala Val Ser             320 325 330 cct gga agc cag atg tca cct gat ctg act cca aag agc gcc ctg gat 1117 Pro Gly Ser Gln Met Ser Pro Asp Leu Thr Pro Lys Ser Ala Leu Asp         335 340 345 gca tct ttg tcc cct gtt cct tac atg ttt ggg cga gtg aga aaa aca 1165 Ala Ser Leu Ser Pro Val Pro Tyr Met Phe Gly Arg Val Arg Lys Thr     350 355 360 ggt gca gtt ctg gag aaa gtg att gag aga agg caa aaa agg atg ata 1213 Gly Ala Val Leu Glu Lys Val Ile Glu Arg Arg Gln Lys Arg Met Ile 365 370 375 380 aag aat agg gaa tca gct gca aga tcc cgc gct cgc aag caa gct tat 1261 Lys Asn Arg Glu Ser Ala Ala Arg Ser Arg Ala Arg Lys Gln Ala Tyr                 385 390 395 acg atg gaa ctg gaa gca gaa att gcg caa ctc aaa gaa ttg aat gaa 1309 Thr Met Glu Leu Glu Ala Glu Ile Ala Gln Leu Lys Glu Leu Asn Glu             400 405 410 gag ttg cag aag aaa caa gtt gaa atc atg gaa aag cag aaa aat cag 1357 Glu Leu Gln Lys Lys Gln Val Glu Ile Met Glu Lys Gln Lys Asn Gln         415 420 425 ctt ctg gag cct ctg cgc cag cca tgg gga atg gga tgc aaa agg caa 1405 Leu Leu Glu Pro Leu Arg Gln Pro Trp Gly Met Gly Cys Lys Arg Gln     430 435 440 tgc ttg cga agg aca ttg acg ggt ccc tgg tagagcttat aatggcgtct 1455 Cys Leu Arg Arg Thr Leu Thr Gly Pro Trp 445 450 aaggaaccca acaaagcgcc gaagttatag aacaactcag aagatagaaa gctagctttg 1515 tacgtagttt aggcaggttc tgtgggtgat tgtaaatctt gaagtgtggc ggatttgaca 1575 gagatagata aacacatatc tgttctattt tcctaaatct tttggtttta tcttcctgat 1635 gtaatggatc tttatcattt gtcttgaaca tctttgtgac ttaaccagag tgaatttatc 1695 tt 1697 <210> 68 <211> 454 <212> PRT <213> Arabidopsis thaliana <400> 68 Met Gly Ser Arg Leu Asn Phe Lys Ser Phe Val Asp Gly Val Ser Glu   1 5 10 15 Gln Gln Pro Thr Val Gly Thr Ser Leu Pro Leu Thr Arg Gln Asn Ser              20 25 30 Val Phe Ser Leu Thr Phe Asp Glu Phe Gln Asn Ser Trp Gly Gly Gly          35 40 45 Ile Gly Lys Asp Phe Gly Ser Met Asn Met Asp Glu Leu Leu Lys Asn      50 55 60 Ile Trp Thr Ala Glu Glu Ser His Ser Met Met Gly Asn Asn Thr Ser  65 70 75 80 Tyr Thr Asn Ile Ser Asn Gly Asn Ser Gly Asn Thr Val Ile Asn Gly                  85 90 95 Gly Gly Asn Asn Ile Gly Gly Leu Ala Val Gly Val Gly Gly Glu Ser             100 105 110 Gly Gly Phe Phe Thr Gly Gly Ser Leu Gln Arg Gln Gly Ser Leu Thr         115 120 125 Leu Pro Arg Thr Ile Ser Gln Lys Arg Val Asp Asp Val Trp Lys Glu     130 135 140 Leu Met Lys Glu Asp Asp Ile Gly Asn Gly Val Val Asn Gly Gly Thr 145 150 155 160 Ser Gly Ile Pro Gln Arg Gln Gln Thr Leu Gly Glu Met Thr Leu Glu                 165 170 175 Glu Phe Leu Val Arg Ala Gly Val Val Arg Glu Glu Pro Gln Pro Val             180 185 190 Glu Ser Val Thr Asn Phe Asn Gly Gly Phe Tyr Gly Phe Gly Ser Asn         195 200 205 Gly Gly Leu Gly Thr Ala Ser Asn Gly Phe Val Ala Asn Gln Pro Gln     210 215 220 Asp Leu Ser Gly Asn Gly Val Ala Val Arg Gln Asp Leu Leu Thr Ala 225 230 235 240 Gln Thr Gln Pro Leu Gln Met Gln Gln Pro Gln Met Val Gln Gln Pro                 245 250 255 Gln Met Val Gln Gln Pro Gln Gln Leu Ile Gln Thr Gln Glu Arg Pro             260 265 270 Phe Pro Lys Gln Thr Thr Ile Ala Phe Ser Asn Thr Val Asp Val Val         275 280 285 Asn Arg Ser Gln Pro Ala Thr Gln Cys Gln Glu Val Lys Pro Ser Ile     290 295 300 Leu Gly Ile His Asn His Pro Met Asn Asn Asn Leu Leu Gln Ala Val 305 310 315 320 Asp Phe Lys Thr Gly Val Thr Val Ala Ala Val Ser Pro Gly Ser Gln                 325 330 335 Met Ser Pro Asp Leu Thr Pro Lys Ser Ala Leu Asp Ala Ser Leu Ser             340 345 350 Pro Val Pro Tyr Met Phe Gly Arg Val Arg Lys Thr Gly Ala Val Leu         355 360 365 Glu Lys Val Ile Glu Arg Arg Gln Lys Arg Met Ile Lys Asn Arg Glu     370 375 380 Ser Ala Ala Arg Ser Arg Ala Arg Lys Gln Ala Tyr Thr Met Glu Leu 385 390 395 400 Glu Ala Glu Ile Ala Gln Leu Lys Glu Leu Asn Glu Glu Leu Gln Lys                 405 410 415 Lys Gln Val Glu Ile Met Glu Lys Gln Lys Asn Gln Leu Leu Glu Pro             420 425 430 Leu Arg Gln Pro Trp Gly Met Gly Cys Lys Arg Gln Cys Leu Arg Arg         435 440 445 Thr Leu Thr Gly Pro Trp     450 <210> 69 <211> 1717 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (234) .. (1379) <400> 69 aaaaaaaaaa aaaactgaac tctttttcgc tctggttttt ttagagagag agaaagatga 60 aaatgcgttt aattgctgtt taggtttcga attcgcgatt taaatttctg ggtttctctc 120 tgtttaagct tcttcttctt catcttctgc ttacgtttct tcttcaagga gctttcggat 180 tcttgtagaa agagtcattg ttctcttgag tgggaaacct tgaaaccatt cct atg 236                                                            Met                                                              1 gga aat agc agc gag gaa cca aag cct cct acc aaa tca gat aaa cca 284 Gly Asn Ser Ser Glu Glu Pro Lys Pro Pro Thr Lys Ser Asp Lys Pro               5 10 15 tct tca ccc ccg gtg gat caa aca aat gtt cat gtc tac cct gat tgg 332 Ser Ser Pro Pro Val Asp Gln Thr Asn Val His Val Tyr Pro Asp Trp          20 25 30 gca gct atg cag gca tat tat ggt cca aga gta gca atg cct cct tat 380 Ala Ala Met Gln Ala Tyr Tyr Gly Pro Arg Val Ala Met Pro Pro Tyr      35 40 45 tac aat tca gct atg gct gca tct ggt cat cct cct cct cct tac atg 428 Tyr Asn Ser Ala Met Ala Ala Ser Gly His Pro Pro Pro Pro Tyr Met  50 55 60 65 tgg aat cct cag cat atg atg tca cca tat gga gca ccc tat gct gct 476 Trp Asn Pro Gln His Met Met Ser Pro Tyr Gly Ala Pro Tyr Ala Ala                  70 75 80 gtt tat cct cat gga gga gga gtt tac gct cat ccc ggt att ccc atg 524 Val Tyr Pro His Gly Gly Gly Val Tyr Ala His Pro Gly Ile Pro Met              85 90 95 gga tca ctg cct caa ggt caa aag gat cca cct tta aca act ccg ggg 572 Gly Ser Leu Pro Gln Gly Gln Lys Asp Pro Pro Leu Thr Thr Pro Gly         100 105 110 acg ctt ttg agc atc gac act cct act aaa tct aca ggg aac aca gac 620 Thr Leu Leu Ser Ile Asp Thr Pro Thr Lys Ser Thr Gly Asn Thr Asp     115 120 125 aat gga ttg atg aag aag ctg aaa gag ttt gat ggg ctt gct atg tct 668 Asn Gly Leu Met Lys Lys Leu Lys Glu Phe Asp Gly Leu Ala Met Ser 130 135 140 145 cta gga aat ggg aat cct gaa aat ggt gca gat gaa cat aaa cga tca 716 Leu Gly Asn Gly Asn Pro Glu Asn Gly Ala Asp Glu His Lys Arg Ser                 150 155 160 cgg aac agc tca gaa act gat ggt tct act gat gga agt gat ggg aat 764 Arg Asn Ser Ser Glu Thr Asp Gly Ser Thr Asp Gly Ser Asp Gly Asn             165 170 175 aca act ggg gca gat gaa ccg aaa ctt aaa aga agt cga gag gga act 812 Thr Thr Gly Ala Asp Glu Pro Lys Leu Lys Arg Ser Arg Glu Gly Thr         180 185 190 cca aca aaa gat ggg aaa caa ttg gtt caa gct agc tca ttt cat tct 860 Pro Thr Lys Asp Gly Lys Gln Leu Val Gln Ala Ser Ser Phe His Ser     195 200 205 gtt tct ccg tca agt ggt gat acc ggc gta aaa ctc att caa gga tct 908 Val Ser Pro Ser Ser Gly Asp Thr Gly Val Lys Leu Ile Gln Gly Ser 210 215 220 225 gga gct ata ctc tct cct ggt gta agt gca aat tcc aac ccc ttc atg 956 Gly Ala Ile Leu Ser Pro Gly Val Ser Ala Asn Ser Asn Pro Phe Met                 230 235 240 tca caa tct tta gcc atg gtt cct cct gaa act tgg ctt cag aac gag 1004 Ser Gln Ser Leu Ala Met Val Pro Pro Glu Thr Trp Leu Gln Asn Glu             245 250 255 aga gaa ctg aaa cgg gag cga agg aaa cag tct aat aga gaa tct gct 1052 Arg Glu Leu Lys Arg Glu Arg Arg Lys Gln Ser Asn Arg Glu Ser Ala         260 265 270 aga agg tca aga tta agg aaa cag gcc gag aca gaa gaa ctt gct agg 1100 Arg Arg Ser Arg Leu Arg Lys Gln Ala Glu Thr Glu Glu Leu Ala Arg     275 280 285 aaa gtg gaa gcc ttg aca gcc gaa aac atg gca tta aga tct gaa cta 1148 Lys Val Glu Ala Leu Thr Ala Glu Asn Met Ala Leu Arg Ser Glu Leu 290 295 300 305 aac caa ctt aat gag aaa tct gat aaa cta aga gga gca aat gca acc 1196 Asn Gln Leu Asn Glu Lys Ser Asp Lys Leu Arg Gly Ala Asn Ala Thr                 310 315 320 ttg ttg gac aaa ctg aaa tgc tcg gaa ccc gaa aag aga gtc ccc gca 1244 Leu Leu Asp Lys Leu Lys Cys Ser Glu Pro Glu Lys Arg Val Pro Ala             325 330 335 aat atg ttg tct aga gtt aag aac tca gga gct gga gat aag aac aag 1292 Asn Met Leu Ser Arg Val Lys Asn Ser Gly Ala Gly Asp Lys Asn Lys         340 345 350 aac caa gga gac aat gat tct aac tct aca agc aaa ttg cat caa ctg 1340 Asn Gln Gly Asp Asn Asp Ser Asn Ser Thr Ser Lys Leu His Gln Leu     355 360 365 ctc gat acg aag cct cga gct aaa gca gta gct gca ggc tgatcgatgg 1389 Leu Asp Thr Lys Pro Arg Ala Lys Ala Val Ala Ala Gly 370 375 380 taattcatgt cgatttctac ttaatttgtc gacataaaca aagaaaataa gtgctactaa 1449 tttcagaaaa acttgataga tagtatagta gagagagaga gagagagaga ggtgtgatga 1509 ttattgatct ataaattttc ggagagagag agggagaaag agaaactttt cctccagatg 1569 aaaatttggt gttatggttt gttactgtta atatagagag gcttttcttt ttttataaaa 1629 tggcttcctt tgttgcattt ccttgtttta gacctgatgt aattttatga aatcggtgtt 1689 attgctttgc gtaaaaaaaa aaaaaaaa 1717 <210> 70 <211> 382 <212> PRT <213> Arabidopsis thaliana <400> 70 Met Gly Asn Ser Ser Glu Glu Pro Lys Pro Pro Thr Lys Ser Asp Lys   1 5 10 15 Pro Ser Ser Pro Pro Val Asp Gln Thr Asn Val His Val Tyr Pro Asp              20 25 30 Trp Ala Ala Met Gln Ala Tyr Tyr Gly Pro Arg Val Ala Met Pro Pro          35 40 45 Tyr Tyr Asn Ser Ala Met Ala Ala Ser Gly His Pro Pro Pro Pro Tyr      50 55 60 Met Trp Asn Pro Gln His Met Met Ser Pro Tyr Gly Ala Pro Tyr Ala  65 70 75 80 Ala Val Tyr Pro His Gly Gly Gly Val Tyr Ala His Pro Gly Ile Pro                  85 90 95 Met Gly Ser Leu Pro Gln Gly Gln Lys Asp Pro Pro Leu Thr Thr Pro             100 105 110 Gly Thr Leu Leu Ser Ile Asp Thr Pro Thr Lys Ser Thr Gly Asn Thr         115 120 125 Asp Asn Gly Leu Met Lys Lys Leu Lys Glu Phe Asp Gly Leu Ala Met     130 135 140 Ser Leu Gly Asn Gly Asn Pro Glu Asn Gly Ala Asp Glu His Lys Arg 145 150 155 160 Ser Arg Asn Ser Ser Glu Thr Asp Gly Ser Thr Asp Gly Ser Asp Gly                 165 170 175 Asn Thr Thr Gly Ala Asp Glu Pro Lys Leu Lys Arg Ser Arg Glu Gly             180 185 190 Thr Pro Thr Lys Asp Gly Lys Gln Leu Val Gln Ala Ser Ser Phe His         195 200 205 Ser Val Ser Pro Ser Ser Gly Asp Thr Gly Val Lys Leu Ile Gln Gly     210 215 220 Ser Gly Ala Ile Leu Ser Pro Gly Val Ser Ala Asn Ser Asn Pro Phe 225 230 235 240 Met Ser Gln Ser Leu Ala Met Val Pro Pro Glu Thr Trp Leu Gln Asn                 245 250 255 Glu Arg Glu Leu Lys Arg Glu Arg Arg Lys Gln Ser Asn Arg Glu Ser             260 265 270 Ala Arg Arg Ser Arg Leu Arg Lys Gln Ala Glu Thr Glu Glu Leu Ala         275 280 285 Arg Lys Val Glu Ala Leu Thr Ala Glu Asn Met Ala Leu Arg Ser Glu     290 295 300 Leu Asn Gln Leu Asn Glu Lys Ser Asp Lys Leu Arg Gly Ala Asn Ala 305 310 315 320 Thr Leu Leu Asp Lys Leu Lys Cys Ser Glu Pro Glu Lys Arg Val Pro                 325 330 335 Ala Asn Met Leu Ser Arg Val Lys Asn Ser Gly Ala Gly Asp Lys Asn             340 345 350 Lys Asn Gln Gly Asp Asn Asp Ser Asn Ser Thr Ser Lys Leu His Gln         355 360 365 Leu Leu Asp Thr Lys Pro Arg Ala Lys Ala Val Ala Ala Gly     370 375 380 <210> 71 <211> 1554 <212> DNA <213> Arabidopsis thaliana <220> <221> CDS <222> (162) .. (1379) <400> 71 acaaaatatc tctccctcta tctgcaaatt ttccaaagtt gcatcctttc aatttccact 60 cctctctaat ataattcaca ttttcccact attgctgatt catttttttt tgtgaattat 120 ttcaaaccca cataaaaaaa tctttgttta aatttaaaac c atg gat cct tca ttt 176                                               Met Asp Pro Ser Phe                                                 1 5 agg ttc att aaa gag gag ttt cct gct gga ttc agt gat tct cca tca 224 Arg Phe Ile Lys Glu Glu Phe Pro Ala Gly Phe Ser Asp Ser Pro Ser                  10 15 20 cca cca tct tct tct tca tac ctt tat tca tct tcc atg gct gaa gca 272 Pro Pro Ser Ser Ser Ser Tyr Leu Tyr Ser Ser Ser Met Ala Glu Ala              25 30 35 gcc ata aat gat cca aca aca ttg agc tat cca caa cca tta gaa ggt 320 Ala Ile Asn Asp Pro Thr Thr Leu Ser Tyr Pro Gln Pro Leu Glu Gly          40 45 50 ctc cat gaa tca ggg cca cct cca ttt ttg aca aag aca tat gac ttg 368 Leu His Glu Ser Gly Pro Pro Pro Phe Leu Thr Lys Thr Tyr Asp Leu      55 60 65 gtg gaa gat tca aga acc aat cat gtc gtg tct tgg agc aaa tcc aat 416 Val Glu Asp Ser Arg Thr Asn His Val Val Ser Trp Ser Lys Ser Asn  70 75 80 85 aac agc ttc att gtc tgg gat cca cag gcc ttt tct gta act ctc ctt 464 Asn Ser Phe Ile Val Trp Asp Pro Gln Ala Phe Ser Val Thr Leu Leu                  90 95 100 ccc aga ttc ttc aag cac aat aac ttc tcc agt ttt gtc cgc cag ctc 512 Pro Arg Phe Phe Lys His Asn Asn Phe Ser Ser Phe Val Arg Gln Leu             105 110 115 aac aca tat ggt ttc aga aag gtg aat ccg gat cgg tgg gag ttt gca 560 Asn Thr Tyr Gly Phe Arg Lys Val Asn Pro Asp Arg Trp Glu Phe Ala         120 125 130 aac gaa ggg ttt ctt aga ggg caa aag cat ctc ctc aag aac ata agg 608 Asn Glu Gly Phe Leu Arg Gly Gln Lys His Leu Leu Lys Asn Ile Arg     135 140 145 aga aga aaa aca agt aat aat agt aat caa atg caa caa cct caa agt 656 Arg Arg Lys Thr Ser Asn Asn Ser Asn Gln Met Gln Gln Pro Gln Ser 150 155 160 165 tct gaa caa caa tct cta gac aat ttt tgc ata gaa gtg ggt agg tac 704 Ser Glu Gln Gln Ser Leu Asp Asn Phe Cys Ile Glu Val Gly Arg Tyr                 170 175 180 ggt cta gat gga gag atg gac agc cta agg cga gac aag caa gtg ttg 752 Gly Leu Asp Gly Glu Met Asp Ser Leu Arg Arg Asp Lys Gln Val Leu             185 190 195 atg atg gag cta gtg aga cta aga cag caa caa caa agc acc aaa atg 800 Met Met Glu Leu Val Arg Leu Arg Gln Gln Gln Gln Ser Thr Lys Met         200 205 210 tat ctc aca ttg att gaa gag aag ctc aag aag acc gag tca aaa caa 848 Tyr Leu Thr Leu Ile Glu Glu Lys Leu Lys Lys Thr Glu Ser Lys Gln     215 220 225 aaa caa atg atg agc ttc ctt gcc cgc gca atg cag aat cca gat ttt 896 Lys Gln Met Met Ser Phe Leu Ala Arg Ala Met Gln Asn Pro Asp Phe 230 235 240 245 att cag cag cta gta gag cag aag gaa aag agg aaa gag atc gaa gag 944 Ile Gln Gln Leu Val Glu Gln Lys Glu Lys Arg Lys Glu Ile Glu Glu                 250 255 260 gcg atc agc aag aag aga caa aga ccg atc gat caa gga aaa aga aat 992 Ala Ile Ser Lys Lys Arg Gln Arg Pro Ile Asp Gln Gly Lys Arg Asn             265 270 275 gtg gaa gat tat ggt gat gaa agt ggt tat ggg aat gat gtt gca gcc 1040 Val Glu Asp Tyr Gly Asp Glu Ser Gly Tyr Gly Asn Asp Val Ala Ala         280 285 290 tca tcc tca gca ttg att ggt atg agt cag gaa tat aca tat gga aac 1088 Ser Ser Ser Ala Leu Ile Gly Met Ser Gln Glu Tyr Thr Tyr Gly Asn     295 300 305 atg tct gaa ttc gag atg tcg gag ttg gac aaa ctt gct atg cac att 1136 Met Ser Glu Phe Glu Met Ser Glu Leu Asp Lys Leu Ala Met His Ile 310 315 320 325 caa gga ctt gga gat aat tcc agt gct agg gaa gaa gtc ttg aat gtg 1184 Gln Gly Leu Gly Asp Asn Ser Ser Ala Arg Glu Glu Val Leu Asn Val                 330 335 340 gaa aaa gga aat gat gag gaa gaa gta gaa gat caa caa caa ggg tac 1232 Glu Lys Gly Asn Asp Glu Glu Glu Val Glu Asp Gln Gln Gln Gly Tyr             345 350 355 cat aag gag aac aat gag att tat ggt gaa ggt ttt tgg gaa gat ttg 1280 His Lys Glu Asn Asn Glu Ile Tyr Gly Glu Gly Phe Trp Glu Asp Leu         360 365 370 tta aat gaa ggt caa aat ttt gat ttt gaa gga gat caa gaa aat gtt 1328 Leu Asn Glu Gly Gln Asn Phe Asp Phe Glu Gly Asp Gln Glu Asn Val     375 380 385 gat gtg tta att cag caa ctt ggt tat ttg ggt tct agt tca cac act 1376 Asp Val Leu Ile Gln Gln Leu Gly Tyr Leu Gly Ser Ser Ser His Thr 390 395 400 405 aat taagaagaaa ttgaaatgat gactacttta agcatttgaa tcaacttgtt 1429 Asn tcctattagt aatttggctt tgtttcaatc aagtgagtcg tggactaact tattgaattt 1489 gggggttaaa tccgtttctt atttttggaa ataaaattgc tttttgttta aaaaaaaaaa 1549 aaaaa 1554 <210> 72 <211> 406 <212> PRT <213> Arabidopsis thaliana <400> 72 Met Asp Pro Ser Phe Arg Phe Ile Lys Glu Glu Phe Pro Ala Gly Phe   1 5 10 15 Ser Asp Ser Pro Ser Pro Pro Ser Ser Ser Ser Tyr Leu Tyr Ser Ser              20 25 30 Ser Met Ala Glu Ala Ala Ile Asn Asp Pro Thr Thr Leu Ser Tyr Pro          35 40 45 Gln Pro Leu Glu Gly Leu His Glu Ser Gly Pro Pro Pro Phe Leu Thr      50 55 60 Lys Thr Tyr Asp Leu Val Glu Asp Ser Arg Thr Asn His Val Val Ser  65 70 75 80 Trp Ser Lys Ser Asn Asn Ser Phe Ile Val Trp Asp Pro Gln Ala Phe                  85 90 95 Ser Val Thr Leu Leu Pro Arg Phe Phe Lys His Asn Asn Phe Ser Ser             100 105 110 Phe Val Arg Gln Leu Asn Thr Tyr Gly Phe Arg Lys Val Asn Pro Asp         115 120 125 Arg Trp Glu Phe Ala Asn Glu Gly Phe Leu Arg Gly Gln Lys His Leu     130 135 140 Leu Lys Asn Ile Arg Arg Arg Lys Thr Ser Asn Asn Ser Asn Gln Met 145 150 155 160 Gln Gln Pro Gln Ser Ser Glu Gln Gln Ser Leu Asp Asn Phe Cys Ile                 165 170 175 Glu Val Gly Arg Tyr Gly Leu Asp Gly Glu Met Asp Ser Leu Arg Arg             180 185 190 Asp Lys Gln Val Leu Met Met Glu Leu Val Arg Leu Arg Gln Gln Gln         195 200 205 Gln Ser Thr Lys Met Tyr Leu Thr Leu Ile Glu Glu Lys Leu Lys Lys     210 215 220 Thr Glu Ser Lys Gln Lys Gln Met Met Ser Phe Leu Ala Arg Ala Met 225 230 235 240 Gln Asn Pro Asp Phe Ile Gln Gln Leu Val Glu Gln Lys Glu Lys Arg                 245 250 255 Lys Glu Ile Glu Glu Ala Ile Ser Lys Lys Arg Gln Arg Pro Ile Asp             260 265 270 Gln Gly Lys Arg Asn Val Glu Asp Tyr Gly Asp Glu Ser Gly Tyr Gly         275 280 285 Asn Asp Val Ala Ala Ser Ser Ser Ala Leu Ile Gly Met Ser Gln Glu     290 295 300 Tyr Thr Tyr Gly Asn Met Ser Glu Phe Glu Met Ser Glu Leu Asp Lys 305 310 315 320 Leu Ala Met His Ile Gln Gly Leu Gly Asp Asn Ser Ser Ala Arg Glu                 325 330 335 Glu Val Leu Asn Val Glu Lys Gly Asn Asp Glu Glu Glu Val Glu Asp             340 345 350 Gln Gln Gln Gly Tyr His Lys Glu Asn Asn Glu Ile Tyr Gly Glu Gly         355 360 365 Phe Trp Glu Asp Leu Leu Asn Glu Gly Gln Asn Phe Asp Phe Glu Gly     370 375 380 Asp Gln Glu Asn Val Asp Val Leu Ile Gln Gln Leu Gly Tyr Leu Gly 385 390 395 400 Ser Ser Ser His Thr Asn                 405 <210> 73 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: primer <400> 73 cgccagggtt ttcccagtca cga 23 <210> 74 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: primer <400> 74 agcggataac aatttcacac agga 24

[0095]

[Sequence Listing Free Text] SEQ ID NOs: 73 and 74 are synthetic primers.

[Brief description of drawings]

FIG. 1 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL08-16-G17.

FIG. 2 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-11-M11.

FIG. 3 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-11-M11.

FIG. 4 is a characteristic diagram showing the relationship between high salt stress and expression ratio of RAFL06-11-K21.

FIG. 5 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL06-11-K21.

FIG. 6 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL06-08-H20.

FIG. 7 is a characteristic diagram showing the relationship between high salt stress and expression ratio of RAFL06-08-H20.

FIG. 8 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-16-H23.

FIG. 9 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-16-H23.

FIG. 10 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL08-16-D06.

FIG. 11 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL07-10-G04.

FIG. 12 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL04-17-D16.

FIG. 13 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-19-M20.

FIG. 14 is a characteristic diagram showing the relationship between high salt stress and expression ratio of RAFL08-11-M13.

FIG. 15 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL04-15-K19.

FIG. 16 is a characteristic diagram showing the relationship between low temperature stress and expression ratio of RAFL04-15-K19.

FIG. 17 is a characteristic diagram showing the relationship between drought stress and expression ratio for RAFL05-11-L01.

FIG. 18 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-11-L01.

FIG. 19 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-14-C11.

FIG. 20 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-19-G24.

FIG. 21 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-19-G24.

FIG. 22 is a characteristic diagram showing the relationship between low temperature stress and expression ratio of RAFL05-19-G24.

FIG. 23 is a characteristic diagram showing the relationship between drought stress and expression ratio for RAFL05-20-N02.

FIG. 24 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-18-H12.

FIG. 25 is a characteristic diagram showing the relationship between high salt stress and expression ratio of RAFL05-18-H12.

FIG. 26 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio for RAFL05-19-E19.

FIG. 27 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL06-10-D22.

FIG. 28 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL06-12-M01.

FIG. 29 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL06-12-M01.

FIG. 30 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-14-D24.

FIG. 31 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-14-D24.

FIG. 32 is a characteristic diagram showing the relationship between low temperature stress and expression ratio of RAFL05-20-N17.

FIG. 33 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-20-N17.

FIG. 34 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL04-17-F21.

FIG. 35 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL09-12-N16.

FIG. 36 is a characteristic diagram showing the relationship between drought stress and expression ratio of AFL05-19-I05.

FIG. 37 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-19-I05.

FIG. 38 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-21-I22.

FIG. 39 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL08-11-H20.

FIG. 40 is a characteristic diagram showing the relationship between high salt stress and expression ratio of RAFL08-11-H20.

FIG. 41 is a characteristic diagram showing the relationship between high salt stress and expression ratio of RAFL05-21-C17.

FIG. 42 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-21-C17.

FIG. 43 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-08-D06.

FIG. 44 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL05-20-M16.

FIG. 45 is a characteristic diagram showing the relationship between high salt stress and expression ratio of RAFL05-20-M16.

FIG. 46 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL11-01-J18.

FIG. 47 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL11-01-J18.

FIG. 48 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL11-09-C20.

FIG. 49 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL05-18-N16.

FIG. 50 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL11-10-D10.

FIG. 51 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio of RAFL11-10-D10.

FIG. 52 is a characteristic diagram showing the relationship between drought stress and expression ratio of RAFL04-17-N22.

FIG. 53 is a characteristic diagram showing a relationship between high salt stress and expression ratio of RAFL04-17-N22.

FIG. 54 is a characteristic diagram showing the relationship between drought stress and expression ratio for RAFL05-09-G15.

FIG. 55 is a characteristic diagram showing the relationship between high salt concentration stress and expression ratio for RAFL05-09-G15.

FIG. 56 is a characteristic diagram showing the relationship between drought stress and expression ratio for RAFL05-21-L12.

FIG. 57 is a characteristic diagram showing a relationship between high salt concentration stress and expression ratio of RAFL05-21-L12.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Miki Fujita             3-1-1 Takanodai, Tsukuba City, Ibaraki Prefecture             Chemical Research Center Tsukuba Research Center F-term (reference) 2B030 AD04 CA15 CA17 CA19                 4B024 AA08 BA80 CA04 DA01 GA11                       HA12                 4B065 AA88X AA89X AA89Y AB01                       AC14 BA02 CA24 CA53

Claims (8)

[Claims] 1. A gene encoding an environmental stress-responsive transcription factor, which comprises the following amino acid sequence (a) or (b): (a) Any amino acid sequence selected from SEQ ID NO: 2n (n = 1 to 36) (b) One or more amino acid sequences selected from SEQ ID NO: 2n (n = 1 to 36) Consisting of an amino acid sequence in which the amino acid of is deleted, substituted or added,
Amino acid sequence showing responsiveness to environmental stress and having transcription factor activity 2. The gene according to claim 1, which comprises the following DNA (a), (b) or (c): (A) DNA consisting of any base sequence selected from SEQ ID NO: 2n-1 (n = 1 to 36) (b) Any selected from SEQ ID NO: 2n-1 (n = 1 to 36) DNA consisting of a nucleotide sequence in which one or more nucleotides have been deleted, substituted or added in the nucleotide sequence of, and coding for an environmental stress responsive transcription factor (c) SEQ ID NO: 2n-1 (n = 1 to 36) DNA that hybridizes with a DNA consisting of any of the nucleotide sequences selected from the following under stringent conditions and encodes an environmental stress-responsive transcription factor 3. The gene according to claim 1 or 2, wherein the environmental stress is at least one selected from the group consisting of low temperature stress, drought stress and salt stress. 4. An expression vector containing the gene according to claim 1 or 2. 5. A transformant containing the expression vector according to claim 4. 6. A transgenic plant containing the expression vector according to claim 4. 7. The transgenic plant according to claim 6, wherein the plant is a plant body, a plant organ, a plant tissue or a plant cultured cell. 8. A method for producing a stress tolerant plant, which comprises culturing or cultivating the transgenic plant according to claim 6 or 7.