JP2003180295A - Carotenoid-formulated food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a carotenoid-formulated food especially useful for proliferation or the like of cancer or lifestyle-related disease by discovering Dunaliella phycobiont containing a large amount of phytoene, and formulating the Dunaliella phycobiontor or the phytoene obtained by culturing the Dunaliella phycobiont. <P>SOLUTION: This carotenoid-formulated food is obtained by formulating the phytoene which is an acyclic carotenoid. The phytoene can be used in a form contained in the Dunaliella phycobiont. The phytoene can also be obtained by culturing the Dunaliella phycobiont. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a carotenoid-containing food containing phytoene contained in an alga of Donaliella.

[0002]

2. Description of the Related Art Conventionally, β-isolated from a Donaliella algae
It is known that carotene is made into a vegetable oil suspension and used as a natural colorant for foods, cosmetics or feeds, or as a substance having a nutritional function or other bioregulatory function.

Also, since the Donaliella algal cells contain proteins, lipids, ash, carbohydrates, iron, vitamins and various other active ingredients, for example, Japanese Patent Publication No. 4-75753.
Japanese Patent Publication No. 4-78268 Japanese Patent Publication No. 4-78268
As disclosed in Japanese Patent Publication No. 269, etc., there are tablets, capsules, and granules in which the Donaliella algal cells themselves are used as health-promoting foods.

[0004]

However, the above-mentioned food containing Donalella algal cells has not been made paying attention to phytoene contained in the Donalella algal cells or obtained by culturing the Donariella algal cells.

Phytoene has recently become known as a carotenoid particularly useful for prevention of cancer and lifestyle-related diseases, and it is contained in tomato, wheat, marigold, etc. So, it shows almost no activity by itself.

[0006] Therefore, the present invention has found that the Donariella algal cells contain a large amount of phytoene, and the Donariella algal cells are mixed with the phytoene obtained by culturing the Donariella algal cells. The purpose of the present invention is to provide a food containing a carotenoid, which is particularly useful for preventing customary diseases.

[0007]

Therefore, the carotenoid-containing food of the present invention contains phytoene, which is an acyclic carotenoid.

In the present invention, phytoene can be contained in the Donaliella algal cells. As the Donaliella algal cells containing phytoene, it is preferable to use those having a high content of 5% or more of phytoene in the Donaliella algal cells, and they can be used as a dry powder without any special processing. As the Donaliella algae, Donariella salina species (Dunaliella salina),
It shall include all species belonging to the genus Donaliella, such as Dunaliella bardawil.

Further, in the present invention, phytoene may be obtained by culturing Donaliella algal cells. To obtain phytoene, nicotine may be added as a productivity regulator, and pH of the medium may be adjusted to culture the Donaliella algal cells in a light irradiation environment. The addition amount of the productivity regulator is preferably 1 μM to 1M. 1
If it is less than μM or exceeds 1 M, the phytoene production capacity is reduced or lost. The medium for culturing the Donaliella algal cells may be any medium as long as it can cultivate the Donaliella algal cells. For example, 1.5 M sodium chloride, 50 mM sodium hydrogen carbonate, 5 mM magnesium sulfate, 2 mM potassium nitrate, 0.3 mM Calcium chloride, 0.2 mM potassium dihydrogen phosphate, 1.5
μM ferric chloride, 6 μM EDTA, 7 μM manganese chloride, 1 μM cupric chloride, 1 μM zinc chloride, 1 μM
A medium containing M cobalt chloride and 1 μM ammonium molybdate can be used. As an environment for culturing the Donaliella algal cells, the culture temperature is preferably in the range of about 25 to 35 ° C., the pH of the medium is preferably about 7 to 8, and the environment of light irradiation of about 2,000 to 40,000 Lux is preferable. preferable. Phytoene obtained by culturing Donaliella algal cells has all isomers including all-trans-type phytoene and 9-cis-type phytoene, all of which prevent cancer, diabetes, liver disease, cancer, and other lifestyle-related diseases. It is a carotenoid useful for the prevention and improvement of

The food form of the carotenoid-containing food of the present invention is preferably any of capsules, tablets, granules and powders. These food forms facilitate the food intake. To form a food form into a capsule, a dry powder of phytoene-containing Donaliella algal cells is granulated and filled into a capsule as it is to form a hard capsule, and a capsule mixed with edible oil is filled into a capsule to form a soft capsule. To make a tablet, a dry powder of phytoene-containing Donaliella algal cells may be mixed with an excipient, a binder or the like and compressed into tablets. Granules may be prepared by mixing dry powder of phytoene-containing Donaliella algal cells with a disintegrating agent, and then dry-sizing.

[0011]

[Examples] Next, preferred examples of the carotenoid-containing food of the present invention will be described.

Example 1 In order to make the carotenoid-containing food of the present invention into a hard capsule, first, 100 kg of phytoene-containing Donariella algal cells dry powder is passed through a 42-mesh sieve and sized. Next, 200 g of vitamin E powder is added to the dried powder of the sized Donariella algae, and the mixture is mixed for 15 minutes with a rocking mixer. This mixture was filled into capsules by 350 mg in a hard capsule filling machine, and the adhesive surface of the capsule was banded with a gelatin sheet to stabilize the phytoene in the capsule.
Stabilized for 4 hours to obtain a product.

(Example 2) To make the carotenoid-containing food of the present invention into soft capsules, first, soybean oil 100 k
Beeswax 10kg, fatty acid glycerin ester 10kg
Stir it while heating it to 60 ℃ and dissolve it with vitamin E5k.
g, add Vitamin C palmitate 0.5kg, 10
Stir for minutes. After cooling to 25 to 30 ° C., 100 kg of phytoene-containing Donariella algal cells dry powder is mixed, mixed and stirred for 30 minutes with a homomixer, finely pulverized with a colloid mill, and sieved with a 50 mesh sieve. The prepared solution that has been sieved is degassed under reduced pressure, transferred to a tank, and the prepared solution has an internal capacity of 350 mg.
Mold into soft capsules. After molding, the product was dried in a drying room for 72 hours to obtain a product.

(Example 3) In order to make the carotenoid-containing food of the present invention into tablets, first, 680 kg of phytoene-containing Donariella algal cells dry powder, and crystalline cellulose 156.
kg, partially pregelatinized starch 120 kg, β-cyclodextrin 108 kg, pullulan 14.4 kg,
Reduced maltose starch syrup 2.4 kg, sucrose fatty acid ester 3.
6 kg is mixed with a high speed mixer for 4 minutes and granulated. The granulated product is dried at 60 ° C. in a fluidized bed drier, pulverized by a power mill and sized. Mix evenly with a V-type mixer, 22
The product sieved with a No. sieve is compressed into tablets of 300 mg.

Next, to coat the plain tablets with a sugar coating, 756.5 kg of granulated sugar and 53 of eggshell calcium
9 kg, calcium carbonate 66 kg, lactose 53 kg, shellac 35.5 kg, gum arabic 20 kg, talc 1
4 kg and 10 kg of gelatin were used, sugar coating was obtained by coating the undercoat with a sugar-coated bread, the middle coating with a high coater, and the gloss with a gloss pan.

[0016]

INDUSTRIAL APPLICABILITY The present invention, which is configured as described above, has found that the Donariella algal cells contain a large amount of phytoene, and the Donariella algal cells are blended with or cultivated. Thus, a carotenoid-containing food containing phytoene, which is particularly useful for preventing cancer and lifestyle-related diseases, can be obtained.

Claims (4)

[Claims] 1. A carotenoid-containing food, which contains phytoene which is an acyclic carotenoid. 2. A carotenoid-containing food, comprising a Donaliella algal cells containing phytoene, which is an acyclic carotenoid. 3. The carotenoid-containing food according to claim 1, wherein the phytoene is obtained by culturing Donaliella algal cells. 4. The carotenoid-containing food according to claim 1 or 2, wherein the food form is any of capsules, tablets, granules, and powders.