JP2003174865A - Method for culturing and cultivating new strain - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new Lyophyllum ulmarium strain containing a bitter component of its fruit body at its harvest in an almost indiscernible extent on serving as a food even by cultivating it in a highly bittering medium, and a method for culturing and cultivating the same. <P>SOLUTION: This new Lyophyllum ulmarium strain fruit body of which obtained by cultivating it in a highly bittering medium has less bitterness than that of 0.000008 M quinine sulfate in at least one kind highly bittering medium is selected from Lyophyllum ulmarium Lu 1-13 (FERM P-12571) or its variant strains. The method for culturing or cultivating the new strain by inoculating the new strain on the medium and generating its mycelium or fruit body is also provided. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a new strain of basidiomycete, more specifically, Lyophyllum ulmarium
and a method for cultivating fruiting bodies of a new strain of yllum ulmarium).

[0002]

[Prior art]

[Patent Document 1] Japanese Unexamined Patent Publication No. 63-273467 SUMMARY OF THE INVENTION Riofilum ulmarium naturally grows in dead trees of various broad-leaved trees in the autumn and grows or dilates.
Due to its shape and crispness, it has been eaten as an extremely delicious dish. In addition, in recent years, using a culture medium containing rice bran and other nutrients in sawdust, a fungal bed artificial cultivation method has been established in which cultivation is performed in bottles or boxes, and mushrooms can be stably harvested throughout the year regardless of the season. It's starting to happen. Briefly explaining artificial bed cultivation of riophyllum ulmarium here, a bottle or box filled with a culture medium and steam sterilized is inoculated with a seed culture in which hyphae have been propagated in the same culture medium beforehand, and cultured for a predetermined number of days. After that, generation operation is performed to obtain fruit bodies and harvest to complete one cycle. Although several strains are commercially available or provided by technical cooperation as strains used for cultivation, there are various forms and cultivation characteristics depending on the strains used (JP-A-63-273).
467).

[0003]

However, all of the currently used Riophyllum ulmarium strains contain bitterness components in the fruiting bodies at the time of harvesting, and are edible in a high bittering medium that increases the yield of fruiting bodies. Has an unsuitable bitterness. In addition, the bitterness component varies depending on the type of medium, and there is a large difference in the bitterness intensity. Generally, mushrooms are mainly used in a vegetable-like manner, which places importance on the taste of the raw material, so that a large amount of bitterness components results in a marked decrease in commercial value. For this reason, the present situation is that even a medium base material that significantly increases the yield of fruiting bodies, a medium that simultaneously increases the bitterness component, that is, a high bitterness medium cannot be used. In view of the present situation, the object of the present invention is to cultivate and culture these new bitterness strains with little bitterness when the bitterness component in the fruiting body at harvest is edible even when cultivated in these high bittering medium. To provide a method.

[0004]

Means for Solving the Problems To outline the present invention, the first invention of the present invention is an invention relating to a new strain of Riofilum ulmarium, in which the bitterness of fruiting bodies obtained by culturing in a high bittering medium is No less bitter than 0.000008M quinine sulphate, and at least one high bittering medium, and Riofilum ulmarium Lu 1-13
(FERM P-12571) or a mutant strain thereof, which is a new strain of Riofilum ulmarium. The second invention of the present invention is Riofilum.
The invention relates to a method for culturing a mycelium of a new strain of ulmarium, which is characterized in that the new strain of lyophilum ulmarium of the first invention is inoculated into a medium to form a mycelium. A third invention of the present invention is an invention relating to a method for cultivating fruiting bodies of a new strain of Riophyllum ulmarium, which is characterized by inoculating a medium with the new strain of Riofrum ulmarium new strain and forming a fruiting body. To do.

The present inventors conducted a sensory test on the fruiting body of Riophyllum ulmarium to clarify the relationship between the bitterness of the fruiting body and the cultivation medium, and artificially cultivate it using a medium for increasing the bitterness of the fruiting body, that is, a high bittering medium. The present invention succeeded in breeding a new strain of Riofilum ulmarium having a bitterness of the fruiting body which is less bitter than that of 0.000008M quinine sulfate in at least one type of highly bittered medium, and completed the present invention.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be specifically described below. As lyophilum ulmarium strain
Riofilum Ulmarium Lu 1-2 (FERM P-12584) described in JP-A-3-273467;
Riofilum Ulmarium Lu 1-8 (FERM
BP-1416), Riofilum Ulmarium L
u 1-17 (FERM BP-1417), riophyllum ulmarium M-8171 (FERM BP-
1415) was used to artificially cultivate each strain according to the method described in the publication, and fruit bodies at a suitable harvesting time were harvested, and the fruit body was subjected to a sensory test. As a medium for artificial cultivation, 50 g of coniferous sawdust and 50 broadleaf sawdust described in the publication are used.
g, rice bran 90g well mixed, water content 6 in tap water
The product prepared at 5% was pressed into a polypropylene 850 ml wide-mouthed bottle, and a hole with a diameter of 1 cm was formed facing downward from the center of the bottle mouth. The medium subjected to autoclaving for 60 minutes was used as medium A. In addition, according to the method for preparing the culture medium, 130 g of corn cob meal, 60 g of mamekawa and 30 g of bran were mixed in the same manner to prepare B medium, 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 100 g of rice bran, and the same. C medium, coniferous sawdust 1
D medium was prepared by similarly mixing 00 g and 70 g of Memekawa and similarly prepared. Table 1 shows the yield (g / bottle) of fruiting bodies and sensory test results obtained in each medium used.
Shown in.

[0007]

[Table 1]

In the sensory test, lyophilum ulmarium fruit bodies are stir-fried in oil, and quinine sulfate is used as a standard substance for bitterness by a panelist. Fried oil is performed as follows. That is, the base of the obtained fruit body of Riofilum ulmarium is removed, divided into individual pieces, lightly washed with water, and well drained. Next, add 5m of salad oil to the frying pan.
Add 1 liter, spread all over and heat oil well over medium heat. Riofilum ulmarium fruiting body 100g (wet weight)
Add the ingredients to the pan and stir with chopsticks for 2 minutes or more, and heat the whole mixture. When the pan is dry, turn off the heat. Allow the fruiting body to cool to room temperature and not bitter the panelist,
The four levels of bitterness, pretty bitterness, and remarkably bitterness are evaluated.
In the following, − in each table means no bitterness, + means bitterness, ++ means much bitterness, and ++ means remarkably bitterness. This +
The middle of − is the threshold of bitterness with quinine sulfate as a standard substance, which is the bitterness of Riofilum ulmarium fruiting body, that is,
It is a bitterness with almost no bitterness, the threshold value of quinine sulfate as a standard substance, 0.000008M (Shizuyuki Ota, "Knowledge of food seasoning" 2nd edition, 1st edition, October 1, 1985,
Corresponds to the bitterness of Koshou Shobo, page 38). Among the above-mentioned test strains, riophyllum ulmarium Lu 1-2 is the strain with the lowest bitterness, but when B, C or D medium is used, the yield increases but the fruiting body obtained has a bitter taste. .

In the present invention, the high bitterness medium means a medium in which the bitterness of fruiting bodies obtained when artificially cultivating Riophyllum ulmarium Lu 1-2 is + or higher by a sensory test, for example, the above B and C. , D medium. Further, the new strain of the present invention means a strain in which the bitterness of fruiting bodies obtained by cultivating the strain in a high bittering medium is less bitter than 0.000008M quinine sulfate in at least one type of high bittering medium.

Next, breeding of the new strain of the present invention will be described. 1. Selective breeding Riofilum ulmarium 11 occurring in the natural world
Tissue separation was carried out from the two fruiting bodies to obtain a purely separated mycelium 90 strain. Next, this 90 strain was subjected to a cultivation test using the B medium. By the cultivation test, fruit bodies of 48 strains that formed fruit bodies were subjected to a sensory test. Ten strains with excellent fruiting body shape, high yield, and low bitterness were selected. Further, these 10 strains were subjected to a cultivation test in B, C and D media, and Riofilum ulmarium Lu 1-13 was selected as a strain having the lowest bitterness and suitable for industrial cultivation.

The riophyllum ulmarium will be described below. Riofilum Ulmarium Lu
The 1-13 strain was isolated by the present inventors from a fruit body that had been crowded in a dead tree in Urabandai, Fukushima Prefecture, and the characteristics of the fruit body and spores are as follows. Fruit bodies are crowded, lumps are 5 to 1.5 cm in diameter, round or irregular round mountain shape, surface is smooth, moist, white to brownish cream color, with slightly dark patches, often showing old age. May have a central crack. The flesh is white and has a wide variety of patterns. The handle is eccentric and curved, 3 to 7 x 1 to 2 cm, almost the same color as the umbrella, white at the top, fluffy or powdery. The spores were almost spherical, smooth, colorless, 4.5 to 5.5 × 3.5 to 4.5 μm, and the pattern was white. Comparing the above characteristics with the description in Seiya Ito, Vol. 5, No. 5 of the Japanese Journal of Mycological Fungi (1955, Yokendo Publishing), it is clear that this bacterium is Riofilum ulmarium.

Next, Riofilum ulmarium Lu
The various bacteriological properties of strains 1-13 are shown below. (1) Malt extract agar medium (25 ° C culture) Day 7: vigorous growth. The colony diameter is 40 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 10: Mycelia grow on the entire petri dish. Day 17: Dense aerial mycelia develop on the entire surface. Mycelia are white. (2) Potato-glucose agar medium (25 ° C. culture) Day 7: vigorous growth. The colony diameter is 36 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Day 10: Mycelia grow on the entire petri dish. Day 17: Dense aerial mycelia develop on the entire surface. Light yellow in the center of the colony, white in others. (3) Czapek-Dox agar medium (25 ° C. culture) Day 7: Small growth. The colony diameter is 26 mm. Very few mycelia and aerial hyphae that grow in a dendritic form and are extremely thin. Day 17:
Mycelia grow on the entire dish. Mycelium is dendritic, thin and white. (4) Sabouraud agar medium (25 ° C. culture) Day 7: vigorous growth. The colony diameter is 42 mm. White, cotton-like dense hyphae, aerial hyphae. Day 10: Mycelia grow on the entire petri dish. An abundant amount of aerial mycelia is produced, and the hyphae are cotton-like and white. (5) Oatmeal agar medium (25 ° C. culture) Day 7: Vigorous growth. The colony diameter is 36 mm. Hyphae are well branched and elongated, and aerial hyphae are few. Day 10: Mycelia grow on the entire petri dish. A large amount of cotton-like aerial hyphae are produced.
Mycelia are white. (6) Synthetic Mucor agar medium (25 ° C. culture) Day 7: Small growth. The colony diameter is 21 mm. The hyphae are white and linearly elongated, forming radial colonies.
Day 17: Mycelia grow on the entire petri dish. A large amount of aerial hyphae are produced. Mycelia are white. (7) YpSs agar medium (25 ° C. culture) Day 7: vigorous growth. The colony diameter is 40 mm. A large amount of white, dense hyphae and aerial hyphae are produced. Matte shape. Day 10:
Mycelia grow on the entire dish. A large amount of dense aerial hyphae are produced. The mycelium is white, but the medium turns yellow. (8) Phenol oxidase assay medium (25 ° C. culture) Day 7: Small growth. The colony diameter is 18 mm. Mycelium is white and grows short and mat-like with few aerial mycelia. The medium is browned and the browning radius is 40 mm. Day 17: Medium growth. Colony diameter is 37 mm. The browning radius is 42 mm. The growth rate significantly varies depending on the old and new seeds. (9) Optimal growth temperature A PGY agar medium was inoculated with a discoid inoculum having a diameter of 5 mm, cultured at each temperature for 12 days, and then the colony diameter was measured. As a result, the optimum growth temperature was around 25 ° C. Also,
Almost no growth occurred at 5 ° C and 35 ° C. (10) Optimal growth pH PGY liquid medium (PGY agar without agar) 60
Each ml was dispensed into a 100 ml Erlenmeyer flask for sterilization, the pH was adjusted to each pH with an acid or an alkali, and inoculated with an inoculum. As a result, the optimum growth pH was 7-8. Moreover, the pH range in which growth was possible was pH 3.5 to 10.

Next, Riofilum ulmarium Lu
Based on the taxonomic fact that the hyphae are different hyphae, if the sexes possessed by both hyphae are different from each other, the sex Differences were examined by inversion culture on agar. The tested Riofilum ulmarium is Riofilum ulmarium IFO 963
7. Riofilum Ulmarium IFO 3052
5. Riofilum Ulmarium IFO 3077
5, Riofilum ulmarium Lu 1-2, Riofilum ulmarium Lu 1-8, Riofilum ulmarium Lu 1-17, Riofilum ulmarium M-8171, and Riofilum ulmarium SA. Riofilum Ulmarium SA
Is an isolate from a commercially available fruit body of Riofilum ulmarium. Each of the above-mentioned binuclear hyphae of Riofilum ulmarium was cut out as a block of 3 × 3 × 3 mm from the preserved slant, and each was inoculated in the center of the PGY agar medium so as to face the binuclear mycelia of Riofilum ulmarium Lu 1-13. (2 cm interval), 25 ° C, 1
After culturing for 4 days, it was determined whether a band line was formed between the colonies of both strains. The results are shown in Table 2. (+ If banding occurred, -if not).

[0014]

[Table 2]

As can be seen from Table 2, all of the above-mentioned strains produced zonal lines in the confrontation culture with Riophyllum ulmarium Lu1-13, and it is clear that Riofilum ulmarium Lu1-13 is a new strain. . As described above, as a new strain by selective breeding of the present invention, for example, Riofilum ulmarium L
u 1-13, but the bitterness of the fruiting bodies obtained by culturing in a high bittering medium as in the above strain is less bitter than 0.000008M quinine sulfate for at least one high bittering medium. The strains shown in are all belonging to the present invention.

As described above, the new strain according to the present invention has a bitterness of fruiting bodies which is less than or equal to the bitterness of at least one high bittering medium even when cultivated in the high bittering medium during artificial cultivation. It has the characteristic that it is stable regardless of the type of medium and has little bitterness in fruiting bodies. Table 3 shows the bitterness and the yield of fruiting bodies in a suitable harvesting period, which was obtained by artificially culturing the new strains A, B, C and D of the present invention. The results of the above-mentioned Riofilum ulmarium SA are also shown in Table 3 together.

[0017]

[Table 3]

As shown in Table 3, the lyophilum ulmarium Lu 1-13 strain does not show bitterness in a conventional artificially culturable strain even when a medium in which fruiting bodies have a bitterness at a suitable harvesting time is used, and fruiting body yield However, the fruiting body without bitterness can be stably obtained in a high yield even when using a high bittering medium which has been difficult to use in the past, since it also increases the bitterness.

The mycelium produced by inoculating the above-mentioned strain into a medium does not show bitterness and is suitable for use in foods. It should be noted that lyophilium ulmarium mycelium has a large amount of dietary fiber and is known to have an anti-cancer effect, and foods using the mycelium are particularly useful for maintaining health.

The new strain bryophyllum ulmarium Lu 1-13 bred in the present invention is Lyophyllum ulm.
It is displayed as arium Lu 1-13, and the Institute of Microbial Science and Technology of the Agency of Industrial Science and Technology, Micromachine Research Institute No. 12571 (FER
It has been deposited as MP-12571).

The bitterness component in the fruiting body of riophyllum ulmarium can also be quantified as follows. Riofilum ulmarium Edible part in fruiting body (kasa part,
The handle) is freeze-dried, and this is crushed to obtain a fruit-body freeze-dried powder. 0.5 g (dry weight) of this lyophilized powder is extracted by shaking with 35 ml of ethyl acetate at 25 ° C. for 24 hours, filtered, concentrated under reduced pressure, and dissolved in 1 ml of methanol while heating to obtain an extracted sample. From the above sample 200 μl,
C [Pharmacia Fine Chemicals (Pharmacia
Fine Chemicals)] is used to separate the bitter component fractions as follows. The column is Lober RP-8 [Merck (M
erck), φ1.0 × 24 cm], and solvent 85
% Methanol (Kanto Chemical; for liquid chromatography), 1.0 ml /
Min, room temperature, detection wavelength 210 nm, elution time 1
Fractions from 0 to 45 minutes are collected. The separated fraction was again concentrated under reduced pressure, dissolved in 1.0 ml of a solvent of methanol: ethanol: water = 3: 4: 4, and 50 μl thereof was subjected to HPLC.
The bitterness component fraction is collected using [LC-6A system manufactured by Shimadzu Corporation]. As the column, μ Bondapak C18 (manufactured by Waters, φ0.39 × 30 cm) was used, and a solvent of methanol: ethanol: water = 3: 4: 4 (Kanto Chemical; methanol for liquid chromatography, Nacalai Tesque;
Special grade ethanol), 0.7 ml / min, temperature 40 ° C., detection wavelength UV 210 nm, elution time 32.5-4
Measure the dry weight of the 4.0 minute fraction. The component eluted in this fraction (hereinafter abbreviated as fraction A) has a strong bitterness in the sensory test, and the bitterness of Riofilum ulmarium fruiting body is attributed to this fraction A.

The cotton seed shells used in the medium of the present invention are
The present invention has been found by the present inventors to be a substrate that can be obtained at low cost and that significantly increases the yield of mushrooms and is useful for artificial cultivation of mushrooms. When this cottonseed shell is used in a medium, it may be mixed with another medium base material or used alone. 15% of cottonseed husks based on dry weight of medium
When used at -60%, the effect of increasing the yield is the best, but the amount used is not limited to this. Mushrooms that can be cultivated in good yield using this cottonseed shell as a medium include, for example, liophyllum ulmarium of the present invention, shiitake mushroom,
There are oyster mushrooms, enoki mushrooms, maitake mushrooms, and the like. For example, strains of these mushrooms may be used for ordinary artificial cultivation.

[0023]

EXAMPLES Examples of artificial cultivation of a new strain of ryophilum ulmarium according to the present invention are shown below, but the present invention is not limited to the scope of the following examples.

Example 1 Liquid PGY medium (PGY agar containing no agar) 10
To 0 ml, Riofilum ulmarium Lu 1-13
(FERM P-12571) and inoculated at 25 ° C for 10
Liquid culture was obtained by culturing for a day. On the other hand, 130 g of corn cob meal, 60 g of mamekawa and 30 g of bran were mixed well and the water content was adjusted to 62% with tap water.
Pack in a polypropylene 850ml wide-mouthed bottle,
After making a hole having a diameter of 1 cm downward from the center of the mouth of the bottle, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes,
After cooling to room temperature, 20 ml of the liquid inoculum was inoculated. Add the culture medium to 3 in a dark place at 25 ° C and a humidity of 50 to 60%.
After culturing for 5 days, hyphae spread over the entire bottle. Further, the culture was continued for 40 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water is discarded, and the conditions are 15 ° C., humidity 90 to 95%, and illuminance 20 Lux for 9 days. The culturing was continued to obtain a fruiting body primordium, the illuminance was further raised to 200 lux, and the cultivation was continued for 13 days to obtain a fruiting body. The yield of the fruiting body thus obtained was 168 g, and no bitterness was felt in the sensory test.

The sensory test of the fruiting body was conducted as follows. The base of the handle of the fruiting body obtained by the above bottle cultivation was removed, and the fruit body was divided into pieces one by one, lightly washed with water, and well drained. On the other hand, add 5 ml of salad oil to a frying pan, spread over the whole, and heat the oil sufficiently over medium heat, then 100 g of fruit body
(Wet weight) was added, the whole was cooked for 2 minutes or more with stirring with chopsticks, and the pan was fried until there was no water, and the heat was turned off. Cool the stir-fried fruit bodies to room temperature,
The bitterness was judged by 10 panelists using quinine sulfate as a standard substance.

Example 2 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was pressed into a polypropylene 850 ml wide-mouthed bottle, and the diameter was 1 downward from the center of the bottle mouth.
After making a hole of cm, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium Lu 1-13. Add the culture medium to 3 in a dark place at 25 ° C and a humidity of 50 to 60%.
After culturing for 0 days, hyphae were spread over the entire bottle. Further, the culture was continued for 35 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water is discarded, and the conditions are 15 ° C., humidity 90 to 95%, and illuminance 20 Lux for 9 days. The culturing was continued to obtain a fruiting body primordium, the illuminance was further raised to 200 lux, and the cultivation was continued for 13 days to obtain a fruiting body. The yield of the fruiting body thus obtained was 143 g, and no bitterness was felt in the sensory test of the fruiting body.

Example 3 100 g of softwood sawdust and 70 g of Mamekawa were mixed well and the water content was adjusted to 63% with tap water.
Pack in a polypropylene 850ml wide-mouthed bottle,
After making a hole having a diameter of 1 cm downward from the center of the mouth of the bottle, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes,
After cooling to room temperature, Riofilum ulmarium Lu
1-13 solid inoculum was inoculated. Dark place, 25 ° C, humidity 5
When the culture medium is cultured for 30 days under the condition of 0 to 60%,
The mycelium spread throughout the bottle. Further, the culture was continued under the same conditions for 37 days to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C and the humidity was 9
Cultivation was continued for 9 days under conditions of 0 to 95% and an illuminance of 20 lux to obtain a fruiting body primordium, and the illuminance was further increased to 200 lux and culturing was continued for 13 days to obtain a fruiting body. The yield of the fruiting body obtained was 157 g, and no bitterness was felt in the sensory test of the fruiting body.

Control Example 1 130 g of corncob meal, 60 g of mamekawa, 3 of fusuma
0 g was mixed well and the water content was adjusted to 62% with tap water. It was packed into a polypropylene 850 ml wide-mouthed bottle with a diameter of 1 mm downward from the center of the bottle mouth.
After making a hole of cm, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium Lu 1-2. dark place,
When the culture medium was cultured for 35 days under the conditions of 25 ° C. and a humidity of 50 to 60%, hyphae were spread over the entire bottle. Furthermore, the culture was continued for 50 days under the same conditions to obtain a fruiting body-generating group. 1 cm of the upper hypha layer of the fruiting body-generating group was removed, and tap water 20
After adding ml to make the water supply enough, discard excess tap water,
Cultivation was continued for 9 days under the conditions of 15 ° C, humidity of 90 to 95%, and illuminance of 20 lux to obtain fruit body primordia.
It was raised to 00 lux and cultured for 13 days to obtain fruiting bodies. The yield of the fruiting body thus obtained was 178 g, and bitterness was felt in the sensory test of the fruiting body.

Control Example 2 50 g of coniferous sawdust, 50 g of crushed cottonseed shell, 10 rice bran
0 g was mixed well and the water content was adjusted to 63% with tap water. It was pressed into a polypropylene 850 ml wide-mouthed bottle, and the diameter was 1 downward from the center of the bottle mouth.
After making a hole of cm, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes, cooled to room temperature, and inoculated with a solid inoculum of Riofilum ulmarium Lu 1-2. dark place,
When the culture medium was cultured for 30 days under the conditions of 25 ° C. and a humidity of 50 to 60%, hyphae were spread on the entire bottle. Further, cultivation was continued for 55 days under the same conditions to obtain a fruiting body-generating group. 1 cm of the upper hypha layer of the fruiting body-generating group was removed, and tap water 20
After adding ml to make the water supply enough, discard excess tap water,
Cultivation was continued for 9 days under the conditions of 15 ° C, humidity of 90 to 95%, and illuminance of 20 lux to obtain fruit body primordia.
After raising to 00 lux and continuing the culture for 14 days, fruiting bodies were obtained. The yield of the fruiting body obtained was 140 g, and bitterness was felt in the sensory test of the fruiting body.

Control Example 3 100 g of coniferous sawdust and 70 g of Mamekawa were mixed well, and the water content was adjusted to 63% with tap water.
Pack in a polypropylene 850ml wide-mouthed bottle,
After making a hole having a diameter of 1 cm downward from the center of the mouth of the bottle, the culture medium was subjected to high-pressure steam sterilization at 120 ° C. for 60 minutes,
After cooling to room temperature, Riofilum ulmarium Lu
1-2 solid inoculum was inoculated. Dark place, 25 ° C, humidity 50
When the culture medium was cultured for 30 days under the condition of -60%, the mycelium spread to the entire bottle. Further, cultivation was continued for 55 days under the same conditions to obtain a fruiting body-generating group. After removing 1 cm of the upper hypha layer of the fruiting body-generating group and adding 20 ml of tap water to sufficiently supply water, surplus tap water was discarded, and the temperature was 15 ° C. and the humidity was 90.
The culturing was continued for 10 days under the conditions of ˜95% and an illuminance of 20 lux to obtain a fruiting body primordium, and the illuminance was further raised to 200 lux for a further 13 days to obtain a fruiting body. The yield of the fruiting body thus obtained was 153 g, and bitterness was felt in the sensory test of the fruiting body.

[0031]

As described above, according to the present invention, it is possible to obtain Riofilum ulmarium fruiting bodies with reduced bitterness of fruiting bodies even when cultivated using a high bittering medium which could not be used conventionally. It is possible to provide lyophilum ulmarium fruiting bodies and mycelium of high quality with high yield industrially.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Katsuhiko Kusakabe             2257 Noji-cho, Kusatsu City, Shiga Prefecture Takara Ag             Re Co., Ltd. (72) Inventor Manami Kino             2257 Noji-cho, Kusatsu City, Shiga Prefecture Takara Ag             Re Co., Ltd. (72) Inventor Yu Matsui             2257 Noji-cho, Kusatsu City, Shiga Prefecture Takara Ag             Re Co., Ltd. (72) Inventor Hideo Morita             4-10-4 Ichiriyama, Otsu City, Shiga Prefecture F-term (reference) 2B011 AA07 BA06 GA03 GA06 GA10                 4B065 AA71X AC20 BA22 BB26                       BB27 BC32 BC33 BC48 CA41

Claims (3)

[Claims] 1. The bitterness of fruiting bodies obtained by culturing in a high bittering medium is at least one high bittering medium,
0.000008M Less bitter than quinine sulphate and lyophilum ulmarium Lu 1-13 (FE
RM P-12571) or a mutant strain thereof, which is a new strain of Riofilum ulmarium. 2. A method for cultivating a new strain of Riofilum ulmarium, which comprises inoculating a culture medium with the new strain of Riofilum ulmarium according to claim 1. 3. A method for cultivating a fruiting body of a new strain of Riofilum ulmarium, which comprises inoculating a medium with the new strain of Riophyllum ulmarium.