JP2003156491A - Evaluation method of dioxins in combustion product - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a technology capable of measuring, efficiently and more accurately, the toxicity of dioxins included in a combustion product such as exhaust gas, flying ashes, waste water, waste soil or the like generated especially from an incineration equipment or the like. SOLUTION: A sample containing the combustion product is extracted with an organic solvent, and a sulfuric acid treatment wherein concentrated sulfuric acid is applied until the extracted liquid-extract becomes achromatic and two- liquid separation is performed to thereby acquire an organic layer as a toxicity evaluation sample, is performed, and an antigen-antibody reaction is performed to the toxicity evaluation sample.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a dioxin analysis method for quantifying dioxins contained in combustion products.

2. Description of the Related Art Conventionally, as this kind of toxicity evaluation method,
Prepare the liquid to be evaluated for toxicity evaluation, obtain the chemical amount of dioxins contained in the liquid to be evaluated, convert the toxicity into the toxicity of the reference dioxins, and then calculate the total (TEQ, toxicity equivalent amount) ) Is calculated, and the toxicity of the liquid to be evaluated is calculated and evaluated. Here, in order to obtain the TEQ, an antigen-antibody reaction using the dioxins as an antigen is used. That is, the dioxin, the carrier to which the antibody for recognizing and capturing is bound, the evaluation target liquid containing the dioxin is exposed, and the amount of dioxin bound to the carrier is
It is obtained by the luminosity of the fluorescent label or the like applied to the antigen.

[0002]

However, according to the technique described above, the antibody has too high selectivity for recognizing an antigen, and, for example, 2,3,7,8-T4CDD is used.
It binds to (2,3,7,8-tetraclodibenzodioxin) but hardly binds to other similar substances, or to dioxins that are highly toxic but are not very toxic. Coupled well,
In many cases, a substance that exhibits a selective binding property that is completely unrelated to the toxicity to the human body is used, and in many cases a correlation between the degree of toxicity of the chemical substance that binds to the antibody and the binding amount of dioxins cannot be obtained. . Therefore, the current situation is that accurate toxicity cannot be evaluated without knowing the composition of the liquid to be evaluated.

Therefore, attempts have been made to detect dioxins using the Ah receptor and to evaluate toxicity based on the detected amount.

In this method, since Ah receptor originally binds to dioxins and then exerts toxicity by acting on DNA, accurate toxicity evaluation can be performed by knowing the amount of dioxins bound to the Ah receptor. Is expected to be possible. For example, as a method that enables such measurement, Ah receptors are dioxins and Ah Receptor Nucl.
There is a method in which the liquid to be evaluated is mixed with an ear tranlocator (ARNT) under conditions capable of forming a complex, the produced complex is allowed to bind to DNA that selectively binds only to the complex, and the amount of binding is measured. Are known.
As described above, even when the Ah receptor is used, the toxicity of dioxins to humans and the binding to the Ah receptor are often inconsistent, especially when analyzing combustion products, and there is almost no toxicity. It is considered preferable to exclude from the toxicity evaluation sample beforehand an interfering substance that will bind to the Ah receptor.

However, what kind of interfering substance should be excluded depends largely on the type of sample and the production conditions. Generally, acid treatment, alkali treatment,
It is unclear whether the interfering substances can be sufficiently removed by a method such as column chromatography, and it may be considered that the toxicity evaluation was not sufficiently reliable in the end. Moreover, when the sample is unnecessarily subjected to a treatment for removing an interfering substance, there is a problem that the loss of dioxins to be evaluated increases as the process becomes more complicated, the time required for the work increases, and the work efficiency decreases. . Further, such a problem is recognized not only when the Ah receptor is used but also as a general technique that utilizes an antigen-antibody reaction, and a countermeasure is desired.

Therefore, in view of the above-mentioned drawbacks, an object of the present invention is to produce exhaust gas, fly ash, waste water, especially from incinerators, etc.
It is an object of the present invention to provide a technique capable of efficiently and more accurately measuring the toxicity of dioxins contained in combustion products such as waste soil.

[0007]

Means for Solving the Problems The inventors of the present invention investigated the coexisting contaminants in evaluating dioxins contained in combustion products. As a result, benzofluoranthene, benzopyrene, indenopyrene, dibenzoanthracene, dibenzene PAH (Polycyclyl) such as benzopyrene
ic Aromatic Hydrocarbon
(Compounds)) is included in the aforementioned interfering substances,
Although the toxicity was low, it was found that the binding to the Ah receptor was relatively high (see Table 1).

[0008]

[Table 1]

Therefore, these PAHs are removed from the combustion products.
As a result of earnest research for selectively removing the present invention, the present invention has been accomplished. Therefore, the characteristic means of the method for evaluating dioxins in combustion products of the present invention for achieving this object is a method for evaluating dioxins in combustion products for evaluating dioxins contained in the combustion products. , A sample containing combustion products is extracted with an organic solvent, concentrated sulfuric acid is allowed to act until the extracted liquid becomes colorless, and a sulfuric acid treatment step of separating the two liquids to obtain an organic layer as a toxicity evaluation sample is performed. There is an antigen-antibody reaction on a toxicity evaluation sample, and a sample containing combustion products is extracted with an organic solvent, and the extracted extract is contacted with silica gel containing 5 to 15 wt% of silver nitrate, A silver nitrate treatment step of obtaining an organic solution of a toxicity evaluation sample may be performed, and an antigen-antibody reaction may be performed on the toxicity evaluation sample. A mixture process is performed in which a receptor complex-constituting substance containing a receptor that binds to syn is mixed, and the dioxins have a base sequence capable of specifically binding to the receptor complex formed by binding to the receptor. It is preferable to act on the DNA fragment portion.

[Operation and Effect] That is, according to the present inventors, the dioxin contained in the combustion product has the same skeleton as that of the dioxin as described above.
It was found that these PAHs were likely to bind to the Ah receptor. Further, it has been clarified by experiments that these PAHs are easily converted into a water-soluble substance by contact with concentrated sulfuric acid and easily separated into two layers and moved to the water layer. Therefore, when evaluating dioxins contained in the combustion products, when a sample containing the combustion products is extracted with an organic solvent and concentrated sulfuric acid acts,
These PAHs will be separated from dioxins.

Here, as described above, such processing is
While sufficient processing is required, there is a problem that excessive processing leads to a reduction in workability. Therefore, we examined the extent of the necessity of such treatment and found that when concentrated sulfuric acid was allowed to act until the extracted liquid became colorless, analytical results highly correlated with the official analytical method were obtained. , As an indicator of the degree of treatment, it is possible to properly remove interfering substances by visually confirming the degree of decrease of the color components dissolved inevitably in the organic solvent extract from the sample containing combustion products. I found that I could do it. for that reason,
When the above sulfuric acid treatment is carried out, a sulfuric acid treatment step of separating the two liquids to obtain an organic layer as a toxicity evaluation sample is performed, and an antigen-antibody reaction is performed on the toxicity evaluation sample, the antigen specifically acts only on dioxins. Then, it becomes a quantifiable state,
Dioxins can be evaluated more accurately and accurately.

A silver nitrate treatment step in which a sample containing combustion products is extracted with an organic solvent, and the extracted extract is contacted with silica gel containing 5 to 15 wt% of silver nitrate to obtain an organic solution of a toxicity evaluation sample. When the antigen-antibody reaction is performed on the toxicity evaluation sample, the silica gel can be packed in a column and processed by chromatography. The same effect can be obtained by a simple operation. In this case, since the operation is simple,
Interfering substances can be removed more effectively by appropriately setting the amount of silica gel, such as performing chromatography for two consecutive operations.

Furthermore, when carrying out the antigen-antibody reaction,
The toxicity evaluation sample and a receptor complex constituent substance containing a receptor that binds to dioxins (specifically, for example, a dioxin solution sampled from an environment containing dioxin, an Ah receptor and its Ah receptor) (Eg, a combination of a mixed solution with ARNT, which forms a complex when the Ah receptor binds to the dioxin, forming a pair)
When a mixing step is performed in which the dioxins are allowed to act on a DNA fragment portion having a base sequence capable of specifically binding to a receptor complex formed by binding to the receptor, in the mixing system The following reactions occur. First, dioxins bind to the Ah receptor. Then, the Ah receptor bound to the dioxins binds to the ARNT to form a receptor complex capable of binding to a specific DNA fragment part. Here, in this system, a DNA fragment portion having a base sequence capable of binding to the receptor complex is provided in a state of being bound to the piezoelectric vibrator, so that the receptor complex is the DNA fragment portion. Bind to. Then, the receptor complex can be quantified by quantifying the increase in mass of the DNA fragment portion, and the toxicity of dioxins can be evaluated.

Specifically, in the case where a piezoelectric vibrator having a pair of electrodes and forming a vibrating portion is provided, and the DNA fragment portion is fixed to the vibrating portion of the piezoelectric vibrator to constitute a toxicity evaluation device. In addition, the above-described reaction causes the piezoelectric vibrator to increase in weight by the amount of the receptor complex bonded.

Then, when the piezoelectric vibrator is vibrated by an oscillator, the fundamental frequency (F) and the frequency change degree (Δ
F) and increased mass (Δm) have a relationship of ΔF / F = Δm / K
Since (K is an inherent constant determined by the oscillator), the increasing mass (Δm) is Δm = (ΔF / F) * K
Since it is proportional to the frequency change (ΔF), the increased mass can be known by examining the frequency change with a frequency measuring device or the like. In addition, since the more toxic substances are more contained in the increased mass of dioxins and the less toxic substances are less, the mass obtained here is exactly the magnitude of toxicity. It can be said to be a thing. Therefore, the toxicity of the liquid to be evaluated can be evaluated easily and accurately in a short time.

If the receptor is an aryl hydrocarbon (Ah) receptor derived from mouse or guinea pig, it is possible to detect dioxins with high sensitivity as compared with those derived from other organisms. In addition, if the receptor is a human-derived arylhydrocarbon (Ah) receptor, it is particularly useful because it is possible to easily know the effect on the human body, which is particularly problematic, based on the liquids to be evaluated collected from various environments. is there.

It is conventionally known that a liquid to be evaluated containing dioxins is supplied to bind the dioxins to a receptor, and the toxicity of the liquid to be evaluated is evaluated by the weight of the receptor bound to the dioxins. It enables simple and accurate evaluation as compared with the case of evaluation by absorbance due to color development.

In particular, when the above-mentioned toxicity evaluation device is used,
By performing a mixing step of supplying a liquid to be evaluated containing dioxins to the toxicity evaluation device, and a measurement step of measuring the amount of the receptor complex by the vibration frequency of the piezoelectric vibrator, particularly for each type of dioxins Toxicity can be measured directly without quantifying the amount of

[0019]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described below with reference to the drawings. [Toxicity Evaluation Device] As shown in FIG. 1, the toxicity evaluation device used in the toxicity evaluation method of the present invention forms an electrode 2 on the surface of a piezoelectric vibrator 1 and non-bonds a toxic substance to the surface of the electrode 2. DNA fragment part 3 having a nucleotide sequence which does not bind to the inactivated receptor in a state and is capable of binding to the receptor complex formed by binding the toxic substance to the receptor
A vibration portion A, which is coupled to, is provided. An oscillator 4 is provided on the electrode 2 to oscillate the piezoelectric vibrator 1 at a predetermined frequency, and the receptor complex Rha
The change of the oscillation frequency at the time of the quantification is configured to be measurable by using the oscillation frequency measuring device 5. Gold is used for the electrode 2. The electrode 2 is preferably made of at least one metal selected from platinum, gold and silver. A crystal oscillator is used as the piezoelectric oscillator 1. As the piezoelectric vibrator 1, it is preferable to use a vibrator selected from barium titanate, potassium sodium tartrate, and quartz. The combination of the type, size, and shape of the electrode 2 and the piezoelectric vibrator 1 is appropriately selected according to the purpose of use and the environment. The electrode 2 may be manufactured by a conventional manufacturing method such as a plating method, a vapor deposition method, or a coating method. Further, the oscillator 4 and the oscillation frequency measuring device 5 may be appropriately selected from known ones.

As the DNA fragment portion 3, for example, one having the base sequences of Sequence 1 and Sequence 2 in the Sequence Listing can be used, and an Ah receptor recognition site can be formed by annealing. In addition, USP5529899
Various base sequences described in Japanese Patent Publication No. 1994-242242 can be synthesized or isolated and used.

[0021] [Sequence 1] GATCCGGAGT TGCGTGAGAA GAGCCA 26

[0022] [Sequence 2] TGGCTCTTCT CACGCAACTC CGGATC 26

As the Ah receptor, those derived from various organisms can be used. For example, human-derived Ah receptors can be used.
Receptors can be used. Such human-derived Ah receptor proliferates cells derived from human hepatoma and
It is obtained from the supernatant by adding EDG buffer, homogenizing and centrifuging. Alternatively, it can be synthesized using recombinant E. coli. The mouse or guinea pig-derived Ah receptor can be obtained from the supernatant by removing the liver, mincing, adding a HEDG buffer, homogenizing, and centrifuging.

To bind the DNA fragment portion to the electrode, 3,3'-dithiodipropionic acid (see Chemical Formula 1),
Alternatively, it can be bound using avidin or biotin.

[0025]

## STR1 ## _{HOOC-CH 2 -CH 2 -S-} S-CH 2 -C
H ₂ -COOH

[Toxicity Evaluation Method] In the toxicity evaluation method of the present invention, (1) dioxins contained in a sample containing combustion products are extracted with an organic solvent. (Extraction Process) (2A) Concentrated sulfuric acid is allowed to act in the separatory funnel until the extracted liquid becomes colorless. The organic layer obtained by separating the two liquids is used as the toxicity evaluation sample. (Sulfuric acid treatment step) (2B) The extracted extract is brought into contact with silica gel containing 5 to 15 wt% of silver nitrate to obtain an organic solution of a toxicity evaluation sample. (Silver Nitrate Treatment Step) The sulfuric acid treatment step (2A) and the silver nitrate treatment step (2B) may be performed either alone or in combination. The sulfuric acid treatment step can also be performed as follows. (2C) The extracted extract is brought into contact with silica gel containing 20 to 50 wt% concentrated sulfuric acid to obtain an organic solution of a toxicity evaluation sample. (Sulfuric acid treatment step (column)) (3) An antigen-antibody reaction is performed on the toxicity evaluation sample. At this time, if necessary, the solvent is distilled off and the solvent is exchanged by re-dissolving in DMSO or the like to more selectively dissolve the dioxins.

As the antigen-antibody reaction in (3), a toxicity evaluation sample containing dioxins is supplied to bind the toxic substance to a receptor, and the toxicity of the liquid to be evaluated is determined by the weight of the receptor bound to the toxic substance. The so-called Ah immunoassay (AhIA) is carried out. Specifically, it is as follows. (3A) A dioxin solution sampled from a dioxin-containing environment, and an ARNT that forms a complex when the Ah receptor and the Ah receptor are paired and the Ah receptor binds to the dioxin ( The electrode part of the above-mentioned toxicity evaluation device is immersed in a mixed solution with a receptor complex constituent substance which constitutes the complex. (Mixing Step) (3B) Piezoelectric vibrators connected to the electrodes are oscillated and the vibration frequency thereof is measured. (Frequency measurement process)

Based on the vibration frequency (frequency change (ΔF)) measured here, the amount of the receptor complex is obtained as the increasing mass (Δm) by the following equation. Δm = (ΔF / F) * K (K is a constant) Here, it is a fundamental frequency (F) frequency variation (ΔF) when the piezoelectric vibrator is vibrated by an oscillator.

Therefore, the toxicity evaluation device enables the relative values of the receptor weights to be compared by the frequency change (ΔF) depending on the solution to be evaluated, and the toxicity can be evaluated.

[0030]

Embodiments of the present invention will be described below with reference to the drawings. [Toxicity Evaluation Device] First, a pair of gold electrodes (diameter 3 mm) is provided on a crystal oscillator (diameter 5 mm) by vapor deposition. After ultrasonically cleaning the gold electrode, it is immersed in the 3,3′-dithiodipropionic acid solution (1 mM, pH 6.4) at room temperature for 1 hour to form a DNA fragment binding site on the gold electrode. At this time, one end of the 3,3′-dithiodipropionic acid is taken into the gold crystal interface, and the other end is held exposed in the solution and firmly fixed. Next, a 10 mM solution of avidin derived from egg yolk is allowed to act on the other end of the 3,3′-dithiodipropionic acid for 1 hour at room temperature to bind the avidin and modify the surface of the gold electrode with avidin. On the other hand, by annealing, Ah
Biotin is bound to (the 5'end of) the DNA fragment portion where the receptor recognition site is formed. This reaction was carried out by adding biotin 10 to a 10 mM solution of the DNA fragment part.
The mM solution is added dropwise at 4 ° C. for 1 hour to act. next,
The biotin-bonded DNA fragment solution (1 mM, pH 7.5) is allowed to act on the avidin-modified gold electrode at 4 ° C. for 24 hours. Then, due to the strong avidin-biotin interaction, the DNA fragment part was attached to the surface of the gold electrode with 3,3′-dithiodipropionic acid-avidin-.
It can be bound and immobilized via biotin. The toxicity evaluation device is obtained by connecting the oscillator and the oscillation frequency measuring device to the pair of electrodes thus formed.

[Toxicity Evaluation Method] (1A) The extraction process was performed by A manufactured by Nippon Dionex Co., Ltd.
SE200 was used under the following conditions. Sample extraction cell: A diatomaceous earth having a thickness of 5 mm was placed inside a stainless steel cylindrical closed cell having a capacity of 33 ml, and an incinerated ash sample or a mixture of 2 g of incinerated waste soil and 0.4 g of diatomaceous earth was placed on the cell. It is prepared by filling the space in the cell with glass beads.

[0032] Stationary (extraction) time: 10 minutes Solvent delivery after standing: 60 vol% Purge (N2 gas): 60 seconds Number of repetitions: 2 times Pressure (pressure on the sample container): 2000psi Oven temperature: 150 ° C Solvent: Toluene 100%

When the sample generated from the incinerator is extracted in this manner, generally 50 ml of a brownish brown extract is obtained, and it is considered that all dioxins contained in the sample are dissolved. .

(1B) When the exhaust gas is applied as a sample, the filter part is used to neutralize the alkali content in the collected dust and to efficiently extract dioxins from the inside of the ash by using 1 g of dust. On the other hand, hydrochloric acid (2 mol / L) was added so that the hydrochloric acid content was 20 mmol or more, and the mixture was treated with hydrochloric acid, and then extracted using the above-mentioned ASE200 under the same conditions.
Put the filtrate and the absorption liquid of the liquid collection part and the washing liquid into the analysis funnel,
Liquid-liquid shaking extraction is performed 3 times with 100 ml of dichloromethane for 1 L of the solution. The adsorption and collection part is 300 ml of toluene, and the temperature is 1
Perform reflux extraction at 30 ° C. for 5 hours. The extracts obtained from each collecting part are combined, dehydrated with sodium sulfate, and made into a fixed amount to obtain a crude extract. In this way, when the exhaust gas sample is extracted, generally, about 1 L of a brown extract is obtained,
It is considered that all dioxins contained in the sample are dissolved.

(2A) In the sulfuric acid treatment step, first, the solvent was distilled off from the extracted extract to obtain a hexane 40 ml solution, which was transferred to a separating funnel, to which 10 ml of concentrated sulfuric acid was added,
Shake for 1 to 10 minutes. Further, the sulfuric acid layer is discarded and the sulfuric acid treatment is performed again. Sulfuric acid treatment is carried out until no substantial coloration is observed in the extract (usually 2 to 3 times is sufficient), and the organic layer is washed with water and collected.

(2B) In the silver nitrate treatment step, first, a column is packed with 0.2 g of silica gel and then with 1 g of silica gel containing 10% silver nitrate. The extract was concentrated to about 1 ml, transferred to this column, and eluted with 50 ml of hexane at a dropping rate of 3 ml / min.

(2C) In the sulfuric acid treatment step (column), the column was first packed with 0.2 g of silica gel, and then 44
Prepare the one filled with 3 g of silica gel containing% concentrated sulfuric acid. The extract was concentrated to about 1 ml, transferred to this column, and added with hexane 5 at a dropping rate of 3 ml / min.
Elute with 0 ml.

(2D) The sulfuric acid treatment step (column) and the silver nitrate treatment step can be carried out simultaneously. In this case,
First, a column packed with 0.2 g of silica gel, 3 g of silica gel containing 44% concentrated sulfuric acid, 0.2 g of silica gel and 1 g of silica gel containing 10% silver nitrate is prepared. The extract was concentrated to about 1 ml, transferred to this column, and eluted with 50 ml of hexane at a dropping rate of 3 ml / min.

(3) After the solvent was distilled off from the eluted solution, D
It is dissolved in 40 μl of MSO and subjected to AhIA. When the above-mentioned toxicity evaluation device is not used, the above-mentioned Ah receptor and ARNT are supplied to the wells to which the DNA fragment part 3 is immobilized, and the complex bound to the DNA fragment part is quantified by a color reaction by an enzyme. Similar results can be obtained. At this time, the degree of color development is determined by comparing the absorbance with respect to a standard substance having a known concentration with the absorbance with respect to the enzyme color development of the complex.

Thus, the correlation between the DEQ value obtained by performing AhIA and the TEQ value (official method) obtained by mass spectrometry on various combustion product samples is as shown in FIGS. Table 2 summarizes the high correlations under each processing condition.

[0041]

[Table 2]

As a result, from the results of 1 to 8, any processing condition (2A, 2B, 2C, 2A + 2B, 2A + 2C,
Even 2A + 2D, 2A + 2D + 2D) shows a high correlation with the actual TEQ value as compared with the case of no treatment (none), and it can be seen that the toxicity evaluation by AhIA becomes more reliable. From the viewpoint of 5 to 8, there is no significant difference in the improvement of the correlation depending on the combination of each treatment, but it can be read that there is room for further improvement in the treatment of soil and exhaust gas. Considering the difference of 11, although the effect can be enhanced by appropriately setting the degree of treatment depending on the concentration of dioxins in the sample, excessive treatment is not effective and the minimum treatment amount is set. By doing so, it can be seen that the reliability can be improved.

According to the above-mentioned dioxin evaluation method, the reactivity of the Ah receptor corresponds favorably to I-TEF, and it is understood that the toxicity evaluation is rational (see Table 1). In addition, I-TEF is a toxicity equivalent count artificially determined by a biological test or the like,
Predetermined amount of 2,3,7,8-tetrachlorodibe
When the toxicity of nzodioxine is set to 1, it shows the toxicity value of the same amount of toxic substance.

Further, here, the Ah receptor and the A
As the RNT mixed solution, the liver was taken out from the guinea pig, minced, 5 ml of HEDG buffer was added per 1 g of the liver, the mixture was homogenized, and centrifuged (12500).
RPM, 4 ° C., 20 minutes), the lipid was removed, the supernatant was taken, and further centrifuged (37,000 RPM, 4 ° C., 60 minutes) to remove the lipid, and the obtained supernatant was used.

[0045]

[Sequence list] <110> Kubota Corporation <120> A method of evaluating dioxin in a combustion
product <130> T099111800 <160> 2 <210> 1 <211> 26 <212> DNA <213> Artificial Sequence <220><223> A DNA fragment having a base sequence capable
of specifically bonding with receptor complexes f
ormed by bonding of dioxins with receptors bonding
with dioxins. <400> 1 gatccggagt tgcgtgagaa gagcca 26 <210> 2 <211> 26 <212> DNA <213> Artificial Sequence <220><223> A DNA fragment having a base sequence capable
of specifically bonding with receptor complexes f
ormed by bonding of dioxins with receptors bonding
with dioxins. <400> 2 tggctcttct cacgcaactc cggatc 26

[Brief description of drawings]

FIG. 1 Schematic diagram of toxicity evaluation device

FIG. 2 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (gray, 2A).

FIG. 3 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (gray, 2B).

FIG. 4 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (gray, 2C).

FIG. 5 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (gray, 2A + 2B).

FIG. 6 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (gray, 2A + 2C).

FIG. 7 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (gray, 2A + 2D).

FIG. 8 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (gray, 2A + 2D + 2D).

FIG. 9 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (soil 1, 2A + 2D).

FIG. 10 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (soil 2, 2A + 2D).

FIG. 11 is a graph showing the correlation between the analysis value by AhIA and the analysis value by mass spectrometry (soil 2, 2A + 2D + 2).
D)

FIG. 12 is a graph showing a correlation between an analytical value by AhIA and an analytical value by mass spectrometry (exhaust gas, 2A + 2D).

[Explanation of symbols]

1 Piezoelectric vibrator 2 electrodes 3 DNA fragment

Front page continuation (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 5/02 G01N 33/00 D 33/00 33/566 33/566 1/28 YF term (reference) 2G052 AA02 AB22 AD12 AD26 AD46 FB05 FD00 GA08 GA11 4D056 AB17 AC01 AC22 BA03 CA06

Claims (3)

[Claims] 1. A method for evaluating dioxins in a combustion product for evaluating dioxins contained in the combustion product, wherein a sample containing the combustion product is extracted with an organic solvent, and the extracted extract is colorless. A method for evaluating dioxins in combustion products, in which a sulfuric acid treatment step is performed in which concentrated sulfuric acid is allowed to act until the solution becomes two, and an organic layer is obtained as a toxicity evaluation sample, and an antigen-antibody reaction is performed on the toxicity evaluation sample. 2. A method for evaluating dioxins in a combustion product for evaluating dioxins contained in the combustion product, wherein a sample containing the combustion product is extracted with an organic solvent, and the extracted liquid is extracted. A method for evaluating dioxins in combustion products, which comprises performing a silver nitrate treatment step of bringing an organic solution of a toxicity evaluation sample into contact with silica gel containing 15% by weight of silver nitrate, and performing an antigen-antibody reaction on the toxicity evaluation sample. 3. The antigen-antibody reaction performs a mixing step of mixing the toxicity evaluation sample and a receptor complex constituent substance containing a receptor that binds to dioxins, and the dioxins bind to the receptor. The method for evaluating dioxins in combustion products according to any one of claims 1 to 2, wherein the method is to act on a DNA fragment having a base sequence capable of specifically binding to the receptor complex formed.