JP2003033191A5 - Recombinant cage protein and precious metal-recombinant cage protein complex - Google Patents
Recombinant cage protein and precious metal-recombinant cage protein complex Download PDFInfo
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- JP2003033191A5 JP2003033191A5 JP2002127922A JP2002127922A JP2003033191A5 JP 2003033191 A5 JP2003033191 A5 JP 2003033191A5 JP 2002127922 A JP2002127922 A JP 2002127922A JP 2002127922 A JP2002127922 A JP 2002127922A JP 2003033191 A5 JP2003033191 A5 JP 2003033191A5
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Description
これらのかご状タンパク質にも、鉄を含む無機物粒子を保持させることが可能である。その他、関連する先行文献として、Levi, S. et al., Biochem. Journal (1996)317,467-473を挙げることができる。 These cage proteins can also retain iron-containing inorganic particles. Other related priorities include Levi, S. et al., Biochem. Journal (1996) 317,467-473 .
Claims (11)
上記組み換えかご状タンパク質の内部にあり貴金属粒子を保持可能な保持部と、
上記保持部と上記組み換えかご状タンパク質の外部とを結ぶトンネル状のチャネルと
を備え、
上記組み換えかご状タンパク質は、アポフェリチンであり、
上記チャネルの内表面における、第1のグルタミン酸(Glu)及び第1のアスパラギン酸(Asp)の存在すべき位置に、分子サイズがグルタミン酸及びアスパラギン酸よりも小さい第1の中性アミノ酸を備えており、
上記保持部の内表面における、第2のグルタミン酸の存在すべき位置に、塩基性アミノ酸または第2の中性アミノ酸をさらに備えている、組み換えかご状タンパク質。A recombinant basket-like protein produced by genetic recombination technology,
A holding part inside the above-mentioned recombinant basket-like protein and capable of holding precious metal particles;
A tunnel-like channel connecting the holding part and the outside of the recombinant basket-like protein,
The recombinant cage protein is apoferritin,
A first neutral amino acid having a molecular size smaller than that of glutamic acid and aspartic acid is provided at a position where the first glutamic acid (Glu) and the first aspartic acid (Asp) should be present on the inner surface of the channel. ,
A recombinant basket-like protein further comprising a basic amino acid or a second neutral amino acid at a position where the second glutamic acid should be present on the inner surface of the holding part .
上記貴金属粒子は金または白金であることを特徴とする組み換えかご状タンパク質。The recombinant basket-like protein according to claim 1 ,
A recombinant basket-like protein, wherein the noble metal particles are gold or platinum.
上記第1の中性アミノ酸がセリン(Ser),アラニン(Ala),グリシン(Gly)のうちから選ばれることを特徴とする組み換えかご状タンパク質。The recombinant basket-like protein according to claim 1 ,
A recombinant cage protein, wherein the first neutral amino acid is selected from serine (Ser), alanine (Ala), and glycine (Gly).
上記塩基性アミノ酸または上記第2の中性アミノ酸が、アルギニン(Arg),リジン(Lys),アラニン(Ala)のうちから選ばれることを特徴とする組み換えかご状タンパク質。The recombinant basket-like protein according to claim 1 ,
A recombinant cage protein, wherein the basic amino acid or the second neutral amino acid is selected from arginine (Arg), lysine (Lys), and alanine (Ala).
上記保持部の内表面には、アミノ酸と置換された1つ以上のシステイン(Cys)が存在することを特徴とする組み換えかご状タンパク質。The recombinant basket-like protein according to claim 1,
A recombinant cage protein characterized in that one or more cysteines (Cys) substituted with amino acids are present on the inner surface of the holding part.
上記組み換えかご状タンパク質の外表面には、システインの存在すべき位置に、当該システインよりも還元機能が小さい物質を備えていることを特徴とする組み換えかご状タンパク質。The recombinant basket-like protein according to claim 1,
A recombinant cage protein characterized in that a substance having a reducing function smaller than that of cysteine is provided on the outer surface of the recombinant cage protein at a position where cysteine should be present.
上記組み換えかご状タンパク質は、貴金属粒子を保持可能な保持部と、上記保持部と上記組み換えかご状タンパク質の外部とを結ぶトンネル状のチャネルとを備え、
上記組み換えかご状タンパク質は、アポフェリチンであり、
上記チャネルの内表面における、第1のグルタミン酸及び第1のアスパラギン酸の存在すべき位置に、分子サイズがグルタミン酸及びアスパラギン酸よりも小さい第1の中性アミノ酸を備えており、
上記保持部の内表面における、第2のグルタミン酸の存在すべき位置に、塩基性アミノ酸または第2の中性アミノ酸をさらに備えている、貴金属−組み換えかご状タンパク質複合体。A noble metal-recombinant cage protein complex composed of noble metal particles and a recombinant cage protein,
The recombinant cage protein comprises a holding part capable of holding noble metal particles, and a tunnel-like channel connecting the holding part and the outside of the recombinant basket protein,
The recombinant cage protein is apoferritin,
A first neutral amino acid having a molecular size smaller than that of glutamic acid and aspartic acid at a position where the first glutamic acid and the first aspartic acid should be present on the inner surface of the channel;
A noble metal-recombinant cage protein complex further comprising a basic amino acid or a second neutral amino acid at a position where the second glutamic acid should be present on the inner surface of the holding part .
外表面上に金または白金粒子を保持していることを特徴とする貴金属−組み換えかご状タンパク質複合体。The noble metal-recombinant cage protein complex according to claim 7 ,
A noble metal-recombinant cage protein complex characterized by holding gold or platinum particles on the outer surface.
上記第1の中性アミノ酸がセリン,アラニン,グリシンのうちから選ばれることを特徴とする貴金属−組み換えかご状タンパク質複合体。The noble metal-recombinant cage protein complex according to claim 7 ,
A noble metal-recombinant cage protein complex, wherein the first neutral amino acid is selected from serine, alanine, and glycine.
上記塩基性アミノ酸または上記第2の中性アミノ酸が、アルギニン,リジン,アラニンのうちから選ばれることを特徴とする貴金属−組み換えかご状タンパク質複合体。The noble metal-recombinant cage protein complex according to claim 7 ,
The noble metal-recombinant cage protein complex, wherein the basic amino acid or the second neutral amino acid is selected from arginine, lysine and alanine.
上記保持部の内表面には、アミノ酸と置換された1つ以上のシステインが存在することを特徴とする貴金属−組み換えかご状タンパク質複合体。The noble metal-recombinant cage protein complex according to claim 7 ,
A noble metal-recombinant cage protein complex characterized in that one or more cysteines substituted with amino acids are present on the inner surface of the holding part.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002127922A JP3719665B2 (en) | 2001-05-14 | 2002-04-30 | Recombinant basket protein and noble metal-recombinant basket protein complex |
Applications Claiming Priority (3)
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JP2001-142983 | 2001-05-14 | ||
JP2001142983 | 2001-05-14 | ||
JP2002127922A JP3719665B2 (en) | 2001-05-14 | 2002-04-30 | Recombinant basket protein and noble metal-recombinant basket protein complex |
Related Child Applications (1)
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JP2005091534A Division JP3756928B2 (en) | 2001-05-14 | 2005-03-28 | Method for producing noble metal-recombinant protein complex |
Publications (3)
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JP2003033191A JP2003033191A (en) | 2003-02-04 |
JP2003033191A5 true JP2003033191A5 (en) | 2005-09-15 |
JP3719665B2 JP3719665B2 (en) | 2005-11-24 |
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JP2002127922A Expired - Fee Related JP3719665B2 (en) | 2001-05-14 | 2002-04-30 | Recombinant basket protein and noble metal-recombinant basket protein complex |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JP3916653B2 (en) | 2004-12-14 | 2007-05-16 | 松下電器産業株式会社 | Method for arranging titanium-binding ferritin and inorganic particles |
US7897408B2 (en) | 2005-09-12 | 2011-03-01 | Japan Science And Technology Agency | Method for producing CdS-apoferritin and ZnS-apoferritin complexes |
JP4291407B2 (en) | 2007-06-15 | 2009-07-08 | パナソニック株式会社 | Ferritin arrangement method and inorganic particle arrangement method |
US20230136993A1 (en) * | 2020-02-04 | 2023-05-04 | Sharp Kabushiki Kaisha | Light-emitting element and method for manufacturing light-emitting element |
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2002
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