JP2003026948A - Red-rice malt pigment - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a red-rice malt pigment strengthened in the stability. SOLUTION: The red-rice malt pigment comprises a red-rice malt pigment in which the main component of the pigments has a molecular weight distribution of more than 6,500, or a red-rice malt pigment which contains, as a ratio of the numbers of the pigments among the constitutional red-rice malt pigments, 50% by volume or more of the pigment main component having a molecular weight of 3,500 or more relative to the number of the whole pigments.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to red yeast rice pigment as a natural red pigment mainly used for coloring red color in foods and cosmetics. The present invention relates to a red yeast rice pigment having enhanced stability.

[0002]

2. Description of the Related Art Red yeast rice pigments are used as natural red pigments for foods and the like in place of synthetic coloring agents in fish paste products, confectionery and the like. However, the red yeast rice pigment is somewhat unstable to light or heat, and improvement in discoloration or fading is desired.

As a current countermeasure, a combination of a paprika dye, annatto dye, or a lac dye with a natural yellow-orange dye that is stable to light and heat can be used. However, it cannot be said to be a sufficient measure.

Therefore, there is a strong demand for improvement in the stability of the red yeast rice pigment itself.

Then, as a result of intensive studies by the present inventors, it was found that the stability of the red yeast rice pigment was related to the molecular weight of the dye main component and its composition ratio, and the present inventors achieved the red yeast rice pigment according to the present invention. .

[0006]

SUMMARY OF THE INVENTION The present invention has been made in view of the above points, and an object of the present invention is to provide a red yeast rice pigment having enhanced stability which has been conventionally demanded.

[0007]

In order to achieve the above object, the red yeast rice pigment according to the present invention comprises a red yeast rice pigment having a pigment main component having a molecular weight distribution of 6500 or more, or a molecular weight as a dye number ratio. Is composed of a red yeast rice pigment containing 50% by volume or more of the dye main component of 3500 or more with respect to the total number of dyes.

Regarding claim 1, the molecular weight distribution is
It can be confirmed by HPLC or gel electrophoresis.

Further, in claim 2, the total number of dyes represents the total amount of dyes in the solution, and is a value obtained by multiplying the dye titer by the capacity.

By having this constitution, the red yeast rice pigment becomes highly stable against light and heat.

[0011] As described in claim 3, claim 1 or 2
The red yeast rice pigment may be a red yeast rice pigment having high light resistance among the red yeast rice pigments described in claim 1 or, as described in claim 4, having high heat resistance among the red yeast rice pigments according to claim 1 or 2. It may be a red yeast rice pigment that is composed of the above.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the red yeast rice pigment according to the present invention will be described below.

The red mold yeast of the present invention is obtained from a culture (solid culture, liquid culture) of Red mold yeast. Since this type of red yeast rice pigment contains monascobulins and ancaflavins as main components, its molecular weight exists in plural.

There are various methods for culturing red mold, and the main components of red mold dyes obtained by these methods show various molecular weight distributions.

In the present invention, a red yeast rice pigment having enhanced stability can be obtained by adjusting the molecular weight distribution of the dye main component constituting the red yeast rice pigment to 6500 or more. Moreover, the stability of the red yeast rice pigment to light and heat can be enhanced by comprising the red yeast rice pigment containing 50% by volume or more of the dye main component having a molecular weight of 3500 or more with respect to the total number of pigments. .

As a means for obtaining these red yeast rice pigments, the red yeast rice pigment obtained by any production method or the red yeast rice pigment separated and purified after production can be applied to the red yeast rice pigment of the present invention.

The red yeast rice pigment of the present invention can also be applied to the red yeast rice pigment preparation prepared by any method from the red yeast rice pigment.

The gist of the present invention is that the dye main component is composed of red yeast rice dye having a molecular weight distribution of 6500 or more, and the dye main component having a molecular weight of 3500 or more as a dye number ratio is 50 volumes with respect to the total number of dyes. It is attributed that the composition of red malt dye contained in an amount of at least 50% has the effect of enhancing the stability of the red malt dye.

[0019]

[Examples] Red yeast rice pigments having various molecular weight distributions were obtained from the following examples. These will be described in detail, but the present invention is not limited to these examples.

Example 1 Using a proteolytic product having a molecular weight distribution of about 100 to 100,000 as a medium, red yeast rice pigment-producing bacteria were cultured. Ethanol and water were added to the obtained culture so that the concentration of ethanol would be 50%, and solid-liquid separation was performed to obtain a red yeast rice pigment solution.

COMPARATIVE EXAMPLE 1 Instead of the protein-degraded product which was the medium used in Example 1, a protein-degraded product having a molecular weight distribution of about 100 to 10,000 was used, and a red mold yeast solution was obtained in the same manner.

Example 2 50% of the proteolytic product which is the medium used in Example 1
% Water-containing ethanol solution, stir it, and let it stand at room temperature for 4 hours.
It was kept at 0 ° C. and left standing for about 24 hours. Then, the precipitate was removed, the concentration of ethanol in the supernatant was adjusted to 75%, and after stirring, the temperature was again kept at 4 ° C. and the mixture was allowed to stand for 24 hours. A precipitate and a supernatant were separated, and the precipitate was lyophilized to be used in place of the proteolytic product as the medium used in Example 1 to obtain a red yeast rice pigment solution in the same manner.

Example 3 The supernatant separated in Example 2 was adjusted to have an ethanol concentration of 80%, stirred, and then kept at room temperature at 4 ° C.
It was left standing for about 4 hours. The precipitate and the supernatant were separated, and the precipitate was lyophilized to be used in place of the proteolytic product as the medium used in Example 1 to obtain a red mold yeast solution in the same manner.

Comparative Example 2 Ethanol was volatilized and removed from the supernatant separated in Example 3 and freeze-dried was used in place of the protein hydrolyzate as the medium used in Example 1, and the same procedure was performed. To obtain a red yeast rice pigment solution.

Examples 1 to 3 and Comparative Examples 1 and 2 described above
The red yeast rice pigment solution thus obtained had an equivalent color value.

The red yeast rice pigment solutions obtained from Examples 1 to 3 and Comparative Examples 1 and 2 and commercially available red yeast rice pigments were used as specimens and applied to the surimi of fish in equal amounts, respectively, and irradiated with light of 3000 lux for 18 hours. The color difference before and after was measured with a color difference meter.

Table 1 shows ΔE values representing the magnitude of color tone change.
Shown in. The values of the samples of Examples 1 to 3 were lower than those of Comparative Examples and commercial products, and the effect of enhancing the stability of red yeast rice pigment was confirmed.

[0028]

[Table 1]

The substance was identified by the following analysis method with respect to the red yeast rice pigment for which the effect of enhancing the stability was recognized. The results are shown in FIG. 1, FIG. 2 and Table 2.

As shown in FIG. 1, SD of the above sample
Analysis was performed by S polyacrylamide gel electrophoresis to compare the molecular weight distributions. Bovine lung-derived aprotinin (manufactured by Sigma-Aldrich) was used as a marker having a molecular weight of 6500, and bovine insulin-derived insulin B chain oxidized form (manufactured by Sigma-Aldrich) was used as a marker having a molecular weight of 3500.

Most of the samples of Examples 1 to 3 in which the effect of enhancing the stability of red yeast rice pigment was recognized were distributed when the molecular weight of the pigment main component was about 6500 or more. On the other hand, in the samples of Comparative Examples 1 and 2 and the commercial products A and B in which the red malt dye has a high stability, almost no distribution was observed at a molecular weight of the dye main component of about 6500 or more, and the molecular weight of about 350
Distribution is observed near 0. This suggests that red yeast rice pigment having a molecular weight distribution of the pigment main component of 6500 or more has high stability.

Next, as shown in FIG. 2, the above samples were analyzed by the HPLC method to further compare the molecular weight distributions. Shodex Asahipak GS-620 HQ (Showa Denko) was used as a column. As the mobile phase, a 0.1 mol / L sodium chloride aqueous solution was used at a flow rate of 0.5 ml / min and an analysis time of 50 minutes. Absorbance at 500 nm was detected with an absorptiometer.

As a marker having a molecular weight of 6500, bovine lung-derived aprotinin (manufactured by Sigma-Aldrich) was used.
The absorbance at 0 nm was detected. A peak was detected at an analysis time of about 20 minutes.

As described above, in the chromatograms of the samples of Examples 1 to 3, which were confirmed to have the effect of enhancing the stability of red yeast rice pigment, the analysis time of about 20 showing the molecular weight distribution of 6500 or more.
Within minutes the main peak was present. On the other hand, Comparative Examples 1 and 2 in which the stability of red yeast rice pigment is not high, and commercial products A and B
The chromatogram of the sample of No. 1 showed a molecular weight distribution of 6500 or less and was 20 minutes or more. HPLC is analyzed in Table 2 and HPL within 20 minutes relative to the total analysis time (50 minutes)
The C area ratio result is shown.

[0035]

[Table 2]

From these SDS polyacrylamide gel electrophoresis and HPLC results, it is suggested that the red yeast rice pigment constituted by the fact that the molecular weight distribution of the red yeast rice pigment is mainly above 6500 has a high stability. . Example 4 1 part of the red malt dye solution obtained from Example 3 was mixed with 1 part of the red malt dye solution obtained from Comparative Example 1 to prepare a new red malt dye solution.

Comparative Example 3 To 2 parts of the red malt dye solution obtained from Comparative Example 1, 1 part of the red malt dye solution obtained from Example 3 was mixed to prepare a new red malt dye solution.

The above Examples 1, 3, 4 and Comparative Example 1,
An equal amount of the red yeast rice pigment solution obtained from No. 3 and a commercially available red yeast rice pigment were applied to the surimi of fish as specimens to obtain 3000.
Lux light was irradiated for 18 hours, and the color difference before and after was measured with a color difference meter.

Table 2 shows ΔE values representing the magnitude of color tone change.
Shown in. As is clear from Table 2, Examples 1, 3,
Sample No. 4 had a lower value than Comparative Examples 1 and 3 and commercial products, and the effect of enhancing the stability of red yeast rice pigment was confirmed.

[0040]

[Table 3]

The red yeast rice pigment solutions obtained from Examples 1, 3 and 4 and Comparative Examples 1 and 3 were used as samples and analyzed by the dialysis equilibrium method to compare the molecular weight distributions. Spectra / Por 7 MWC with dialysis tube molecular weight of about 3500
O: 3500 (manufactured by SPECTRUM LABORATORIES INC.) Was used.

From the sample, red yeast rice pigment was diluted to a certain number of pigments with 50% water-containing ethanol, put in a dialysis tube and dialyzed in 50% water-containing ethanol at a temperature of 25 ° C. After equilibration, the number ratio of the dye main component having a molecular weight of 3500 or more was calculated from the number of red yeast rice dye obtained by measuring the absorbance at 500 nm of the solution outside the tube.

As shown in Table 3, the samples of Examples 1, 3 and 4 in which the effect of enhancing the stability of the red yeast rice pigment was recognized,
Dye main component with molecular weight of 3500 or more is 5 for all dyes
The result was obtained consisting of 0% by volume or more of red yeast rice pigment. On the other hand, Comparative Example 1, in which the stability of red yeast rice pigment is not high
In the sample of No. 3, the dye main component having a molecular weight of 3500 or more in red yeast rice dye was less than 50% by volume with respect to the total number of dyes.
This suggests that the red yeast rice pigment containing 50% by volume or more of the main component of the dye having a molecular weight of 3500 or more as the molecular weight distribution has high stability.

[0044]

[Table 4]

The stability of the red yeast rice pigment described so far has enhanced the light resistance. In the test shown below, the effect of strengthening heat resistance was also confirmed.

A heat resistance test was conducted using the red yeast rice pigment solutions obtained from Examples 2 to 4 and Comparative Examples 1 to 3 and commercially available red yeast rice pigments.

The specimen was diluted with buffer solutions of pH 5, 6 and pH 7 so that the absorbance at 500 nm was about 1. The diluted liquid was heat-treated at a temperature of 98 ° C. for 30 minutes. Then, the absorbance at 500 nm of the liquid before and after the heat treatment was measured, and the dye residual ratio was calculated.

As shown in Table 4, the samples of Examples 2, 3 and 4 in which the effect of light resistance was recognized showed a high dye residual rate. On the other hand, the dye residual ratios of the samples of Comparative Examples 1 to 3 and the commercial products A and B, which do not have high light resistance, were low.

This has a molecular weight distribution of the dye main component of 6500.
It is suggested that the above-mentioned red yeast rice pigment or the red yeast rice pigment containing 50% by volume or more of the dye main component having a molecular weight of 3500 or more with respect to the total number of dyes has high light resistance and high heat resistance. To do.

[0050]

[Table 5]

[0051]

As is apparent from the above description,
The red yeast rice pigment according to the present invention has the following excellent effects. That is, it was confirmed that the red yeast rice pigment having a pigment main component having a molecular weight distribution of 6500 or more has high stability, and that the pigment main component having a molecular weight of 3500 or more as a pigment number ratio is 50% by volume or more based on the total number of pigments. Based on the fact that the red yeast rice pigment containing it has been found to have high stability,
Since the red mold yeast according to the present invention has such a constitution, it is highly stable against light and heat.

Further, as described in claim 3 or 4, for example, depending on the application, a red yeast rice pigment having particularly excellent light resistance or a red yeast rice pigment having particularly excellent heat resistance can be used.

[Brief description of drawings]

FIG. 1 is a schematic diagram illustrating Examples 1 to 3 and Comparative Example 1 according to the present invention
FIG. 3 is an SDS polyacrylamide gel electrophoretic pattern of the red mold yeast dye solution obtained from Example 2 and a commercially available red mold yeast dye. The numerical values shown by the arrows in the figure are molecular weight values by markers.

FIG. 2 shows Examples 1 to 3 and Comparative Example 1 according to the present invention.
It is a chromatogram which shows the HPLC analysis result of the red yeast rice pigment solution obtained from 2 and a commercial red yeast rice pigment.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Atsushi Yamamoto             512-43 Iwami structure, Taishi-cho, Ibo-gun, Hyogo F-term (reference) 4B018 MA05 MC01

Claims (4)

[Claims] 1. The molecular weight distribution of the red yeast rice pigment mainly composed of 6
A red yeast rice pigment, which is composed of 500 or more red yeast rice pigments. 2. A red koji dye, which is composed of a red koji dye containing 50% by volume or more of a dye main component having a molecular weight of 3500 or more as a dye number ratio with respect to the total number of dyes. 3. A red yeast rice pigment, which is one of the red yeast rice pigments according to claim 1 or 2, which has excellent light resistance. 4. A red yeast rice pigment, which is one of the red yeast rice pigments according to claim 1 or 2, which has excellent heat resistance.