JP2002517725A5 - - Google Patents
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- JP2002517725A5 JP2002517725A5 JP2000552498A JP2000552498A JP2002517725A5 JP 2002517725 A5 JP2002517725 A5 JP 2002517725A5 JP 2000552498 A JP2000552498 A JP 2000552498A JP 2000552498 A JP2000552498 A JP 2000552498A JP 2002517725 A5 JP2002517725 A5 JP 2002517725A5
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- Prior art keywords
- solution
- biological material
- slide
- staining
- delivery system
- Prior art date
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- 239000000243 solution Substances 0.000 description 30
- 239000000560 biocompatible material Substances 0.000 description 25
- SQGYOTSLMSWVJD-UHFFFAOYSA-N Silver nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 239000012192 staining solution Substances 0.000 description 11
- 229910001961 silver nitrate Inorganic materials 0.000 description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- WZUVPPKBWHMQCE-VYIIXAMBSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@@]2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-VYIIXAMBSA-N 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- VKYKSIONXSXAKP-UHFFFAOYSA-N Hexamethylenetetramine Chemical compound C1N(C2)CN3CN1CN2C3 VKYKSIONXSXAKP-UHFFFAOYSA-N 0.000 description 4
- RBTARNINKXHZNM-UHFFFAOYSA-K Iron(III) chloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 4
- 239000000908 ammonium hydroxide Substances 0.000 description 4
- 239000000276 potassium ferrocyanide Substances 0.000 description 4
- OCPOWIWGGAATRP-UHFFFAOYSA-N potassium hexacyanoferrate(4-) Chemical compound [K+].[K+].[K+].[K+].N#C[Fe-4](C#N)(C#N)(C#N)(C#N)C#N OCPOWIWGGAATRP-UHFFFAOYSA-N 0.000 description 4
- 235000010339 sodium tetraborate Nutrition 0.000 description 4
- 239000004328 sodium tetraborate Substances 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 210000001519 tissues Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 210000001124 Body Fluids Anatomy 0.000 description 1
- 210000000349 Chromosomes Anatomy 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 241001510071 Pyrrhocoridae Species 0.000 description 1
- 210000003802 Sputum Anatomy 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000004027 cells Anatomy 0.000 description 1
- 210000003850 cellular structures Anatomy 0.000 description 1
- 210000004748 cultured cells Anatomy 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000002538 fungal Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- GIYXAJPCNFJEHY-UHFFFAOYSA-N methyl-[3-phenyl-3-[4-(trifluoromethyl)phenoxy]propyl]azanium;chloride Chemical compound Cl.C=1C=CC=CC=1C(CCNC)OC1=CC=C(C(F)(F)F)C=C1 GIYXAJPCNFJEHY-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 210000000056 organs Anatomy 0.000 description 1
- 244000045947 parasites Species 0.000 description 1
Description
【特許請求の範囲】
【請求項１】 ａ）少なくとも第１および第２の安定な溶液を供し、ここで、該少なくとも第１および第２の安定な溶液は組み合わせた場合に不安定な染色溶液を含み；
ｂ）スライドを供し、ここで、染色されるべき生物学的材料がスライド上に存在し；
ｃ）該少なくとも第１および第２の安定な溶液の所定量をスライド上の生物学的材料に送達するための自動送達システムを供し；
ｄ）該少なくとも第１および第２の安定な溶液を自動送達システムを用いてスライド上の生物学的材料に順次適用し；次いで
ｅ）生物学的材料上で少なくとも第１および第２の安定な溶液を混合する
ことを特徴とするスライド上の生物学的材料を染色する自動方法。
【請求項２】 該混合する工程が渦を形成するために少なくとも２つのガス流を適用することを含む請求項１に記載の方法。
【請求項３】 該生物学的材料が組織セクション、組織培養細胞、細胞小器官、染色体、核酸、炭水化物、脂質および蛋白質を含む細胞成分、血液のスミア、痰および他の体液、分泌物および分泌物類、および寄生虫、ウイルス、細菌および菌類を含めた微生物よりなる群から選択される請求項１に記載の方法。
【請求項４】 該不安定染色溶液が菌類染色溶液、銀染色溶液、鉄染色溶液、鉄ヘマトキシリン、三色染色溶液、ムチン染液、ムチカルミン染色溶液、Ｖｅｒｈｏｆｆ染色溶液、アミロイド染色溶液、およびステイナー（Ｓｔｅｉｎｅｒ
）染色溶液よりなる群から選択される請求項１に記載の方法。
【請求項５】 該混合が、生物学的材料上の少なくとも第１および第２の安定溶液にガス流を適用することによって達成される請求項１記載の方法。
【請求項６】 ａ）約０．２％から約１．０％の硝酸銀の溶液を供し、ここで、該硝酸銀は組み合わせた場合に不安定な染色溶液を含む少なくとも第１および第２の安定な溶液であり、
ｂ）約２．０％から約４．０％のメテナミンの溶液を供し、
ｃ）約０．２％から約０．６％のホウ砂の溶液を供し、
ｄ）スライドを供し、ここで、染色すべき生物学的材料は水平なスライド上に存在し、
ｅ）硝酸銀、メテナミンおよびホウ砂溶液の所定量をスライド上の生物学的材料に送達するための自動送達システムを供し、
ｆ）自動送達システムを用いてスライド上の生物学的材料に硝酸銀、メテナミンおよびホウ砂溶液を順次適用し、次いで、
ｇ）硝酸銀、メテナミンおよびホウ砂溶液を混合して生物学的材料を染色することを特徴とするスライド上の生物学的材料を銀染色する自動方法。
【請求項７】 ａ）約０．２％から約１．０％の硝酸銀の溶液を供し、
ｂ）約０．３％から約１．０％の水酸化アンモニウムの溶液を供し、
ｃ）約０．７％から約１．５％の水酸化ナトリウムの溶液を供し、
ｄ）スライドを供し、ここで、染色すべき生物学的材料は水平なスライド上に存在し、
ｅ）硝酸銀、水酸化アンモニウムおよび水酸化ナトリウム溶液の所定量をスライド上の生物学的材料に送達するための自動送達システムを供し、
ｆ）自動送達システムを用いてスライド上の生物学的材料に硝酸銀、水酸化アンモニウムおよび水酸化ナトリウム溶液を順次適用し、次いで、
ｇ）硝酸銀、水酸化アンモニウムおよび水酸化ナトリウム溶液を混合して生物学的材料を染色する
ことを特徴とするスライド上の生物学的材料を銀染色する自動方法。
【請求項８】 ａ）約０．７％から約１．５％のヘマトキシリン溶液を供し、
ｂ）約０．５％から約１．５％の水性塩化第二鉄の溶液を供し、
ｃ）スライドを供し、ここで、染色すべき生物学的材料は水平なスライド上に存在し、
ｄ）ヘマトキシリンおよび水性塩化第二鉄溶液の所定量をスライド上の生物学的材料に送達するための自動送達システムを供し、
ｅ）自動送達システムを用いてスライド上の生物学的材料にヘマトキシリンおよび水性塩化第二鉄溶液を順次適用し、次いで、
ｆ）ヘマトキシリンおよび水性塩化第二鉄溶液を混合して生物学的材料を染色する
ことを特徴とするスライド上の生物学的材料の三色またはムチカルミン染色の自動方法。
【請求項９】 ａ）約８％から約１２％のフェロシアン化カリウムの溶液を供し、
ｂ）約１５％から約３０％の塩酸の溶液を供し、
ｃ）スライドを供し、ここで、染色すべき生物学的材料は水平なスライド上に存在し、
ｄ）フェロシアン化カリウムの溶液の所定量をスライド上の生物学的材料に送達するための自動送達システムを供し、
ｅ）自動送達システムを用いてスライド上の生物学的材料にフェロシアン化カリウムの溶液を順次適用し、次いで、
ｆ）フェロシアン化カリウムと塩酸の溶液を混合して生物学的材料を染色することを特徴とするスライド上の生物学的材料の鉄染色の自動方法。
[Claims]
1. A) At least first and second stable solutions are provided, where the at least first and second stable solutions include staining solutions that are unstable when combined;
b) Serve the slide, where the biological material to be stained is present on the slide;
c) Provide an automated delivery system for delivering a predetermined amount of the at least the first and second stable solutions to the biological material on the slide.;
d) Sequentially apply the at least the first and second stable solutions to the biological material on the slide using an automated delivery system.Then;
e) Mix at least the first and second stable solutions on the biological material
An automated method of staining biological material on slides, characterized by its ability to stain.
2. The mixing step applies at least two gas streams to form a vortexThe method according to claim 1, which includes the above.
3. The biological material is a tissue section, tissue cultured cells, cell organs, chromosomes, nucleic acids, carbohydrates, cell components including lipids and proteins, blood smears, sputum and other body fluids, secretions and secretions. The method according to claim 1, which is selected from the group consisting of organisms and microorganisms including parasites, viruses, bacteria and fungi.
4. The unstable staining solution is a fungal staining solution, a silver staining solution, an iron staining solution, iron hematoxylin, a tricolor staining solution, a mutin staining solution, a muticalmine staining solution, a Verhoff staining solution, an amyloid staining solution, and a stainer ( Steiner
) The method according to claim 1, which is selected from the group consisting of dyeing solutions.
5. The mixing is achieved by applying a gas stream to at least the first and second stable solutions on the biological material.Claim 1The method described.
6. A) A solution of about 0.2% to about 1.0% silver nitrate is provided, wherein the silver nitrate is at least a first and second stable containing an unstable staining solution when combined. Solution
b) Serve with a solution of about 2.0% to about 4.0% metenamine.
c) Serve with a solution of about 0.2% to about 0.6% borax.
d) Serve slides, where the biological material to be stained is on a horizontal slide,
e) Provide an automated delivery system for delivering a predetermined amount of silver nitrate, methenamine and borax solution to the biological material on the slide.
f) Sequentially apply silver nitrate, methenamine and borax solutions to the biological material on the slide using an automated delivery system, then
g) An automated method of silver staining of biological material on slides, which comprises mixing silver nitrate, methenamine and borax solutions to stain the biological material.
7. A) A solution of about 0.2% to about 1.0% silver nitrate is provided.
b) Provide a solution of about 0.3% to about 1.0% ammonium hydroxide.
c) Provide a solution of about 0.7% to about 1.5% sodium hydroxide.
d) Serve slides, where the biological material to be stained is on a horizontal slide,
e) Provide an automated delivery system for delivering a predetermined amount of silver nitrate, ammonium hydroxide and sodium hydroxide solution to the biological material on the slide.
f) Sequentially apply silver nitrate, ammonium hydroxide and sodium hydroxide solutions to the biological material on the slide using an automated delivery system, then
g) Stain the biological material by mixing silver nitrate, ammonium hydroxide and sodium hydroxide solutions
An automated method of silver staining biological material on slides.
8. a) A hematoxylin solution of about 0.7% to about 1.5% is provided.
b) Provide a solution of about 0.5% to about 1.5% aqueous ferric chloride.
c) Serve slides, where the biological material to be stained is on a horizontal slide,
d) Provide an automated delivery system for delivering a predetermined amount of hematoxylin and aqueous ferric chloride solution to the biological material on the slide.
e) Sequentially apply hematoxylin and aqueous ferric chloride solution to the biological material on the slide using an automated delivery system, then
f) Stain biological material by mixing hematoxylin and aqueous ferric chloride solution
An automated method of tricolor or muticalmin staining of biological materials on slides.
9. a) A solution of about 8% to about 12% potassium ferrocyanide is provided.
b) Provide a solution of about 15% to about 30% hydrochloric acid.
c) Serve slides, where the biological material to be stained is on a horizontal slide,
d) Provide an automated delivery system for delivering a predetermined amount of a solution of potassium ferrocyanide to the biological material on the slide.
e) Sequentially apply a solution of potassium ferrocyanide to the biological material on the slide using an automated delivery system, then
f) An automated method of iron staining of biological materials on slides, characterized in that a solution of potassium ferrocyanide and hydrochloric acid is mixed to stain the biological material.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8767398P | 1998-06-02 | 1998-06-02 | |
| US60/087,673 | 1998-06-02 | ||
| PCT/US1999/012263 WO1999063342A1 (en) | 1998-06-02 | 1999-06-02 | Method for staining biological specimens by combining unstable reagents on a microscope slide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002517725A JP2002517725A (en) | 2002-06-18 |
| JP2002517725A5 true JP2002517725A5 (en) | 2006-01-05 |
Family
ID=22206584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000552498A Pending JP2002517725A (en) | 1998-06-02 | 1999-06-02 | Method for staining biological specimens by combining unstable reagents on microscope slides |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1082611A4 (en) |
| JP (1) | JP2002517725A (en) |
| WO (1) | WO1999063342A1 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6828154B1 (en) | 2000-11-03 | 2004-12-07 | Cytologix Corporation | Staining method with chromic acid precursors |
| DK1771730T3 (en) * | 2004-07-23 | 2014-09-08 | Ventana Med Syst Inc | METHOD AND APPARATUS FOR APPLYING LIQUIDS FOR A BIOLOGICAL TEST |
| CA2764289C (en) | 2004-12-17 | 2013-12-24 | Ventana Medical Systems, Inc. | Methods and compositions for a microemulsion-based tissue treatment |
| CN103415762A (en) * | 2011-01-10 | 2013-11-27 | 文塔纳医疗系统公司 | Hematoxylin staining method |
| CN103743896B (en) * | 2014-01-13 | 2015-04-15 | 南通大学 | Detachable, volume adjustable and evaporation-prevention glass slide incubator |
| CN104678094B (en) * | 2014-01-13 | 2016-04-27 | 南通大学 | The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency |
| CN106018793B (en) * | 2015-05-08 | 2018-02-16 | 河北大学 | A kind of detection method of hexamethylenetetramine |
| CN109238810B (en) * | 2018-11-23 | 2021-06-04 | 中国科学院水生生物研究所 | Rapid preparation method of fresh water ciliate dry silver staining sample |
| KR20230164388A (en) * | 2022-05-25 | 2023-12-04 | 대한민국(관리부서: 행정안전부 국립과학수사연구원장) | Method of footwear impression enhancement on porous surfaces using potassium ferrocyanide solution for footwear impression enhancement |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL135275C (en) * | 1964-07-02 | |||
| US4413584A (en) * | 1978-04-19 | 1983-11-08 | A.J.P. Scientific, Inc. | Biological slide staining apparatus |
| US5225325A (en) * | 1990-03-02 | 1993-07-06 | Ventana Medical Systems, Inc. | Immunohistochemical staining method and reagents therefor |
| DE69117052T2 (en) * | 1990-03-02 | 1996-11-14 | Ventana Med Syst Inc | AUTOMATIC BIOLOGICAL REACTOR |
| US5595707A (en) * | 1990-03-02 | 1997-01-21 | Ventana Medical Systems, Inc. | Automated biological reaction apparatus |
| EP0539379A4 (en) * | 1990-07-18 | 1993-06-02 | Australian Biomedical Corporation Limited | Automatic tissue staining for immunohistochemistry |
-
1999
- 1999-06-02 JP JP2000552498A patent/JP2002517725A/en active Pending
- 1999-06-02 EP EP99955323A patent/EP1082611A4/en not_active Withdrawn
- 1999-06-02 WO PCT/US1999/012263 patent/WO1999063342A1/en not_active Application Discontinuation
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