JP2002370986A - Sugar uptake promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a substance activating sugar uptake in an epidermal cell and resultantly expected to produce promoting effects on cell neogenesis or metabolism in the skin. SOLUTION: This sugar uptake promoter in epidermal cornified cells comprises a purine base or its salt as an active ingredient. The method for promoting the sugar uptake in the epidermal cornified cells is characterized by applying the purine base or its salt to the skin.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sugar uptake promoter having an action of promoting sugar uptake in epidermal keratinocytes (keratinocytes) of the skin. Furthermore, the present invention relates to a method for promoting sugar uptake in epidermal keratinocytes of the skin.

[0002]

2. Description of the Related Art The skin epidermis is composed of various cells such as epidermal keratinocytes (keratinocytes), Langerhans cells and melanocytes from the stratum corneum toward the basal layer.
In cell formation, cell division and differentiation (keratinization) in the epidermis, uptake of sugar in the epidermis, particularly in keratinocytes, has an important role. Therefore, if sugar uptake in epidermal keratinocytes can be activated, skin turnover and metabolism can be promoted, and as a result, pigmentation such as melanin can be prevented or eliminated, cell activation, skin activation It is expected to be effective in preventing aging or preventing or eliminating cell damage caused by ultraviolet rays of epidermal keratinocytes.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a substance capable of activating the sugar uptake of epidermal cells. More specifically, an object of the present invention is to provide a substance that promotes the uptake of sugar, particularly glucose, in epidermal keratinocytes among epidermal cells. Another object of the present invention is to provide a method for promoting the uptake of sugar in epidermal keratinocytes.

[0004]

The present inventors have been studying the promotion of glucose uptake in epidermal cells, and found that purine bases, especially adenosine monophosphate, which is a monophosphate ester of adenosine, showed epidermal angle. It has been found that the cells exhibit an excellent glucose uptake promoting action in activated cells. The present invention has been developed based on such knowledge.

That is, the present invention provides the following sugar uptake promoters: (1) A sugar uptake promoter in epidermal keratinocytes containing purine base or a salt thereof as an active ingredient. (2) at least one purine base selected from the group consisting of adenine, guanine, adenosine monophosphate, adenosine 5'-diphosphate, adenosine 5'-triphosphate, flavin adenine dinucleotide and nicotinamide adenine dinucleotide; The sugar uptake promoter according to (1), which is a seed. (3) The sugar uptake promoter according to (1) or (2), wherein the purine base is adenosine monophosphate. (4) Purine base or a salt thereof is at least 0.1 per 100% by weight of the sugar uptake promoter in epidermal keratinocytes.
The sugar uptake promoter according to any one of (1) to (3), which is contained in a ratio of 1% by weight. (5) The method according to any one of (1) to (4), wherein the purine base or a salt thereof is contained in an amount of 0.1 to 10% by weight per 100% by weight of the sugar uptake promoter in epidermal keratinocytes. Sugar uptake promoter. (6) Purine base or a salt thereof is 0.1 to 7% by weight per 100% by weight of the sugar uptake promoter in epidermal keratinocytes.
The sugar uptake promoter according to any one of (1) to (5), which is contained at a ratio of: (7) The sugar is at least one selected from the group consisting of glucose, arabinose, xylose, mannose, galactose and fructose, (1) to (6).
The sugar uptake promoter according to any one of the above. (8) The sugar uptake promoter according to any one of (1) to (7), which has a pH of 2 to 8. (9) The sugar uptake promoter according to any of (1) to (8), which has an external use form.

Further, the present invention relates to a method for promoting sugar uptake as described below: (10) A method for promoting sugar uptake in epidermal keratinocytes, which comprises applying a purine base or a salt thereof to skin. Method. (11) The purine base is adenine, guanine, adenosine monophosphate, adenosine 5'-diphosphate, adenosine 5'-
(10) The method for promoting sugar uptake according to (10), which is at least one selected from the group consisting of triphosphate, flavin adenine dinucleotide, and nicotinamide adenine dinucleotide. (12) The purine base is adenosine monophosphate (1
The method for promoting sugar uptake according to (0) or (11). (13) The sugar is glucose, arabinose, xylose,
The method of promoting sugar uptake according to any one of (10) to (12), which is at least one selected from the group consisting of mannose, galactose and fructose. (14) The sugar uptake according to any of (10) to (13), wherein a composition containing at least 0.1% by weight of a purine base or a salt thereof is applied to the skin. How to promote. (15) 0.1 to 10% by weight of a purine base or a salt thereof
The method according to any one of (10) to (14), characterized in that a composition containing at a ratio of (1) is applied to the skin. (16) The method according to any one of (10) to (15), wherein a composition containing 0.1 to 7% by weight of a purine base or a salt thereof is applied to the skin. Promotion method. (17) The method for promoting sugar uptake according to any one of (14) to (16), wherein the composition has a pH of 2 to 8.

[0007]

DETAILED DESCRIPTION OF THE INVENTION The agent for promoting sugar uptake in epidermal keratinocytes The agent for promoting sugar uptake in epidermal keratinocytes according to the present invention comprises purine base or a salt thereof as an active ingredient.

[0008] Purine base is a generic term for various derivatives having a purine or purine nucleus as a skeleton. Among them, adenine, guanine, their deaminated products (hypoxanthine, xanthine), adenosine , Guanosine, inosine, adenosine phosphate (adenosine 2'-phosphate, adenosine 3'-phosphate, adenosine 5'-phosphate, adenosine 5'-diphosphate, adenosine 5'-triphosphate), guanosine (Guanosine 3'-phosphate, guanosine 5'-phosphate, guanosine 5'-diphosphate, guanosine 5'-triphosphate), adenylosuccinic acid, xanthic acid, inosinic acid, flavin adenine dinucleotide ( FAD), nicotinamide adenine dinucleotide (NAD) and the like. Among them, purine bases used in the present invention include adenine, guanine, adenosine monophosphate [adenosine 2'-
Phosphate, adenosine 3'-phosphate, adenosine 5'-phosphate (AMP)], adenosine 5'-diphosphate (ADP),
Adenosine 5'-triphosphate (ATP), flavin adenine dinucleotide (FAD), and nicotinamide adenine dinucleotide (NAD) can be preferably exemplified. More preferred is adenosine monophosphate, especially adenosine 5'-phosphate (AMP).

In the present invention, a salt of a purine base can be blended in place of or in combination with the purine base. Examples of the purine base salt include alkali metal salts such as sodium salt and potassium salt; alkaline earth metal salts such as magnesium salt, calcium salt and barium salt; basic amino acid salts such as arginine and lysine; ammonium and tricyclohexyl Examples thereof include ammonium salts such as ammonium salts; various alkanolamine salts such as monoethanolamine, diethanolamine, triethanolamine, monoisopropanolamine, diisopropanolamine, and triisopropanolamine. Preferably, it is an alkali metal salt such as a sodium salt. More preferably, monosodium adenosine monophosphate and disodium adenosine monophosphate can be exemplified.

[0010] The purine base or a salt thereof is 1
The species may be used alone, or two or more kinds may be used in any combination, and the mode of the combination is not particularly limited as long as the effects of the present invention are not hindered.

The above-mentioned purine bases or salts thereof act on the epidermal cells of the skin, and in particular, function to promote the ability of the epidermal keratinocytes to take up sugar. Therefore, the substance exerts its effect by being applied to the skin,
The effect can be used effectively. Therefore, the substance may be used alone as a sugar uptake promoter in epidermal keratinocytes, or may be used as an active ingredient, as well as a pharmaceutically or cosmetically acceptable base, carrier or additive. Skin preparations such as cosmetics and external medicines or quasi-drugs having a sugar uptake promoting action
Can be prepared as In the latter case, the compounding ratio of the purine base or a salt thereof in the external preparation for skin is
There is no particular limitation as long as the effects of the present invention can be achieved. Normally, the final product is a skin external preparation (sugar uptake enhancer)
About 0.1% by weight or more in 100% by weight, preferably about 0.1% by weight.
It can be blended in such a ratio that it is contained in an amount of 5% by weight or more. The upper limit is not limited as long as it has the effect of promoting sugar uptake, which is the effect of the present invention.
% By weight, preferably about 7% by weight, more preferably about 6% by weight.
% By weight. That is, the distribution ratio of the purine base or a salt thereof can be arbitrarily set based on the upper and lower limits. .
1-10% by weight, 0.1-7% by weight, 0.5-10% by weight
Range. From the viewpoint of the stability of the product and the combination of other components, the range is preferably 0.5 to 7% by weight, more preferably 1 to 6% by weight.

[0012] The sugar uptake promoter of the present invention exerts an excellent promoting effect particularly on glucose uptake ability in epidermal keratinocytes. The sugar is not limited to glucose, but may be a monosaccharide such as arabinose, xylose, mannose, galactose or fructose. The sugar uptake promoter of the present invention is a sugar,
In particular, it is sufficient that at least one of the monosaccharides has an action of promoting uptake into epidermal keratinocytes, and it is not necessary that all of the sugars have an action of promoting uptake. More preferred sugars include glucose.

[0013] The sugar uptake promoter of the present invention may contain various components which are usually incorporated into an external preparation as required, for example, a surfactant, a solubilizing component, an oil or fat, a polyhydric alcohol, a thickener, a preservative. Agents, coloring agents, dispersants, pH adjusters, fragrances and the like can be added. In addition, these components are one kind alone,
Alternatively, two or more kinds can be arbitrarily combined and blended.

For example, as the surfactant, any of a cationic surfactant, an anionic surfactant, a nonionic surfactant and an amphoteric surfactant can be used.

Examples of the solubilizing component include lower alcohols such as ethanol; polyhydric alcohols such as glycerin, ethylene glycol and propylene glycol; hydrogenated soybean phospholipids, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene lanolin alcohol, Polyoxyethylene castor oil, polyoxyethylene hardened castor oil, polyoxyethylene sterol, polyoxyethylene alkyl ether, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene alkyl phenyl ether and the like can be mentioned.

Examples of the fats and oils include peanut oil, sesame oil, soybean oil, safflower oil, avocado oil, sunflower oil, corn oil, rapeseed oil, menzite oil, castor oil, camellia oil, coconut oil, and olive oil. Oils, oils such as poppy oil, cacao oil, jojoba oil, tallow, lard, wool oil; hydrocarbon-based liquid oils such as petrolatum, liquid paraffin, squalane, α-olefin oligomer; isopropyl myristate, isopropyl isostearate Myristyl myristate, cetyl palmitate, cetyl isooctanoate, isocetyl myristate, n-butyl myristate, octyl dodecyl myristate, isopropyl linolenate, propyl ricinoleate, isopropyl ricinoleate, isobutyl ricinoleate, heptyl ricinolate, diethyl sebacate , Adipic acid Higher fatty acid esters such as diisopropyl; waxes such as salami beeswax, spermaceti and wood wax; higher fatty alcohols such as cetyl alcohol, stearyl alcohol, behenyl alcohol, bacyl alcohol, and kimyl alcohol; waxes;
Higher fatty acids such as oleic acid and palmitic acid;
Mono-, di-, and triglyceride mixtures of 18 saturated or unsaturated fatty acids; linear silicones such as methylpolysiloxane, dimethylpolysiloxane, methylphenylpolysiloxane, and methylhydrogenpolysiloxane; decamethylcyclopentasiloxane, octamethylcyclotetrasiloxane; Examples thereof include cyclic silicones such as methylcyclosiloxane; crosslinked silicones such as crosslinked methylpolysiloxane and crosslinked methylphenylpolysiloxane; and silicone oils such as modified silicone modified with polyoxyethylene or polyoxypropylene. Preferably, petrolatum, liquid paraffin, squalane, α
-Hydrocarbon-based liquid oils such as olefin oligomers.
When the oil is solid, an auxiliary solubilizer is preferably used.

Examples of the polyhydric alcohol include glycerin, polyglycerin having a polymerization degree of 2 to 10 (eg, diglycerin, triglycerin, tetraglycerin, etc.),
Examples thereof include ethylene glycol, diethylene glycol, polyethylene glycol, 1,3-butylene glycol, propylene glycol, dipropylene glycol, isoprene glycol, pentadiol, sorbitol, maltitol, and fructose.

The sugar uptake promoter of the present invention is obtained by mixing the above-mentioned purine base or a salt thereof with each of the above-mentioned optional components, if necessary, and further adding another solvent or a commonly used external preparation as necessary. Various desired external applications such as paste, mousse, gel, liquid, emulsion, suspension, cream, ointment, sheet, aerosol, spray, etc. It can be prepared in the form of an agent. These can be manufactured by a usual method in the art.

The sugar uptake promoter of the present invention usually has a pH of 2-8.
It is good as long as it has low irritation to skin and mucous membranes,
And from the viewpoint of good skin feel, preferably pH
2-7, more preferably pH 3-7, even more preferably p
Desirably, it is weakly acidic to neutral with H5 to H7.

The concrete form and use of the sugar uptake promoter of the present invention are not particularly limited as long as it is prepared as applied to the skin such as cosmetics, external medicines for skin or quasi-drugs for external use. Not done. For example, makeup cosmetics such as foundation, blusher, lipstick, mascara, eyeshadow, eyeliner, white powder, eyebrow ink and beautiful nail polish; basic cosmetics such as milky lotion, cream, lotion, oil and pack; facial cleanser, cleansing, body It can be prepared as various external compositions such as cleaning agents such as cleaning agents; hair cosmetics such as hair styling agents and hair tonics; it can.
These are used by being applied to the skin in an appropriate amount or an appropriate number of times according to the age, sex, use, and degree of symptoms of the affected skin area of the user.

The sugar uptake promoter of the present invention can promote sugar uptake by epidermal keratinocytes by being applied to, adhered to, or sprayed on the skin. As a result, it is expected to be effective in promoting skin turnover, preventing and improving skin diseases or disorders, skin aging, and the like. In addition, the skin disease and skin disorder mentioned here include pigment (melanin) deposits such as spots, freckles, erythema, sunburn, dullness, and wrinkles.

II. Method for promoting sugar uptake in epidermal keratinocytes As described above, the purine base or a salt thereof can promote sugar uptake in epidermal keratinocytes. That is, the present invention provides a method for promoting sugar uptake in epidermal keratinocytes using the purine base or a salt thereof.

The method of promoting sugar uptake in epidermal keratinocytes of the present invention can be carried out by applying purine base or a salt thereof as it is, or by applying a composition containing these substances to the skin.

In the method of the present invention, the purine base or a salt thereof may be the same as that used in the sugar uptake promoter, and the sugar to be taken up may also be the sugar uptake promoter. Is the same as the sugar to be taken up.

In the method of the present invention, the method of applying the purine base or a salt thereof to the skin is not particularly limited as long as the purine base or a salt thereof is used in a form in contact with the target skin part. For example, purine base or a salt thereof alone or as an active ingredient of these substances,
Alternatively, a composition containing a pharmaceutically or cosmetically acceptable substrate, carrier or additive may be applied or sprayed on the skin, or may be applied in the form of a patch and adhered to the skin. When used in the form of a composition, the mixing ratio of the purine base or a salt thereof to be added to the composition is not particularly limited as long as the effects of the present invention are exhibited. Usually, about 100% by weight of the composition contains about 0.1 purine base or a salt thereof.
%, Preferably about 0.5% by weight or more. The upper limit is not limited as long as the effect of promoting sugar uptake, which is the effect of the present invention, is not limited. For example, about 10% by weight, preferably about 7% by weight.
%, More preferably about 6% by weight. That is, based on the upper and lower limits, the mixing ratio of the purine base or a salt thereof can be arbitrarily set,
As an example, 0.1 to 100% by weight of the composition.
Examples are 10% by weight, 0.1 to 7% by weight, and 0.5 to 10% by weight. The range is preferably 0.5 to 7% by weight, more preferably 1 to 6% by weight. In addition, the composition may have a pH of usually 2 to 8, but preferably has a pH of 2 to 8 from the viewpoint of low irritation to skin and mucous membranes and good usability.
7, more preferably pH 3-7, even more preferably pH 5
It is desirably weakly acidic at ~ 7.

As a specific embodiment of the method of the present invention, various external compositions containing a purine base or a salt thereof (for example, foundation, blusher, lipstick, mascara,
Makeup cosmetics such as eyeshadow, eyeliner, white powder, eyebrow ink and beautiful nails; milky lotion, cream, lotion,
Basic cosmetics such as oils and packs; cleaning products such as facial cleansers, cleansing and body cleaning products; hair cosmetics such as hair styling agents and hair tonics; wound healing agents, wiping agents, detergents, whitening agents, ultraviolet ray protective agents, For example, a method of applying a purine base or a salt thereof to the skin through use of a bath agent or the like can be exemplified.

The amount and number of application of the purine base or its salt are not particularly limited, and include, for example, the type of purine base or its salt, the age of the user, sex, use, degree of symptoms of the affected skin, An appropriate amount of a purine base or a salt thereof can be applied to the skin once or several times a day depending on the application form and the like.

[0028]

EXAMPLES The present invention will be described below with reference to experimental examples and examples, but the present invention is not limited to these examples. In the following formulations,% means% by weight unless otherwise specified.

Experimental Example 1 The effect of purine base on glucose uptake by keratinocytes was examined. An experiment was carried out in the same manner using adenosine 5'-phosphate / 2Na (AMP-2Na), adenosine and inosine as test substances, and using a system containing no test substance as a control. <Process for the preparation of reagents and the like> 1. Preparation of human epidermal keratinocytes Human epidermal keratinocytes (normal human neonatal foreskin epidermal keratinocytes, NHEK (F), manufactured by Kurabo Industries) were cultured once in a 10-cm Petri dish and stored frozen until use. When used, the cryopreserved cells are cultured in a 24-well plate for about 6-7 days,
Once 70-80% confluent, it was used in the experiments. 2.1% BSA / glucose-free medium (pH 7.4)
Preparation of 1 ml of a calcium solution and 0.1 ml of phenol red attached to the medium product were added to a serum-free medium for epidermal keratinocyte growth (Humedia-KG2 custom-made medium, Kurabo Industries) to prepare a medium without glucose. Bovine serum albumin (BSA) from which impurities were removed by activated carbon treatment was added to this medium until the concentration reached 1%, and the pH was adjusted to 7.4 with NaOH. 3. ³ H-2-Preparation of deoxy glucose solution 10 mM 2-deoxyglucose 1ml and 250MyuCi /
250 μl ³ H-2-deoxyglucose 60 μl and 1% BSA
940 μl of a glucose / glucose-free medium (pH 7.4) was mixed.

<Experimental method> Epidermal keratinocytes cultured in a 24-well plate were subjected to PBS (-) (Ca ²⁺ , Mg ²⁺ free phospha).
After washing three times with te buffered saline), a serum-free medium for epidermal keratinocyte proliferation (Humedia-KG2 custom-made medium, Kurabo; no growth additive added) was added thereto, and the cells were cultured for 2 hours. 2
After an hour, the plate was washed three times with PBS (-), and a 1% BSA / glucose-free medium (p.
H7.4) was added thereto and cultured for 30 minutes. After 30 minutes, 20 μl of a ³ H-2-deoxyglucose solution was added per 1 ml of the medium, and the mixture was allowed to stand for 10 minutes, and then washed three times with ice-cold PBS (−) containing glucose. 0.1% SDS
Was added and 0.5 ml of 0.1 N NaOH was added, and the mixture was left standing for 2 hours or more to lyse the cells. 0.11 with 0.1% SDS
0.5 ml of N HCl was added to make the cell lysate acidic. 80 µl of protein quantification (DC Protein Assay, Bi
750 μl was transferred to a glass bottle. Cocktail for liquid scintillation counter (ACS-II,
(Amersham) was added, and the mixture was gently stirred, and the radioactivity was measured with a liquid scintillation counter. From the obtained radioactivity, determined the radioactivity per unit protein content, playing the radioactivity due to nonspecific ³ H-2-deoxyglucose uptake, ³ uptake of H-2-deoxyglucose (H- DPM (μg) / μg protein) was calculated.

<Results> The above experiment was performed twice, and the average value was obtained. Then, in order to compare with the control, each ratio when the amount of ³ H-2-deoxyglucose taken up by the cells in the control was set to 1 was calculated. The results are shown in FIG. In FIG. 1, the horizontal axis represents each purine base (AMP-2).
Na, adenosine, inosine) (log M), the vertical axis represents the amount of 2-deoxyglucose (H-
One control for DPM (μg) / μg protein)
It shows the ratio when it is set as.

As can be seen from FIG. 1, in the first experiment,
AMP-2Na increased the glucose uptake by the control (no test substance added) to about 1.48 at a concentration of 10 ^-6 M.
It was promoted about 1.61 times at 10 ^-5 M concentration. 2
Similarly, in the second experiment, the glucose uptake by the control was promoted by about 1.48 times at the concentration of 10 ⁻⁶ M and about 1.43 times at the concentration of 10 ⁻⁵ M.

Adenosine and inosine are also
Glucose extraction of keratinocytes in the same manner as AMP-2Na
The effect of accelerating the intrusion was confirmed. However, FIG.
As can be seen, at the same concentration of adenosine and AMP-2N
a and glucose uptake promoting action on epidermal keratinocytes
AMP-2Na is more effective at lower concentrations.
Effectively promote glucose uptake,^-6Smell
In the first experiment, AMP-2Na was approximately 1.1% of adenosine.
The promotion effect is as high as 1.32 times in the second and sixth experiments.
(The first experiment: p <0.0)
Fifth and second experiments: p <0.05). In addition, inosine
Therefore, AMP-2Na has a lower concentration (10 ^-7M)
It can be said that glucose uptake is more effectively promoted.

From the above, purine bases such as AMP-2Na, adenosine and inosine have an effect of promoting glucose uptake by keratinocytes, particularly AMP-2.
The effect of Na was confirmed at a lower concentration, indicating that Na was excellent in promoting glucose uptake.

Example 1 Emulsion Carboxyvinyl polymer 0.3 (%) AMP-2Na 1.5 Decaglycerin monomyristate 2.0 Squalane 5.0 Purified glycerin 6.0 pH adjuster Adjusted to pH 7 Preservative Appropriate amount purified Water Residual total 100.0% Example 2 Lotion Polyoxyethylene (EO60) hydrogenated castor oil 0.7 (%) AMP-2Na 3.0 Ethanol 5.0 Glycerin 2.0 pH adjuster Adjust to pH 7 Preservative Agent suitable amount perfume suitable amount purified water residual total 100.0% Example 3 hair restorer salicylic acid 0.1 (%) AMP-2Na 10.0 ethanol 20.0 glycerin 2.0 pH adjuster Adjust to pH7 Preservative Amount Perfume Appropriate amount Purified water Residual total 100.0%

[0036]

According to the present invention, the ability of the epidermal keratinocytes of the skin to take in sugar can be promoted. As a result, an effect of promoting skin turnover and metabolism can be expected. For this reason, the sugar uptake promoter of the present invention is expected to have effects such as prevention of skin aging and skin beautifulness, such as preventing fine wrinkles and giving elasticity to the skin, and preventing pigmentation and maintaining and promoting skin whitening. It is useful as a skin cosmetic, a topical drug or a quasi drug for external use.

[Brief description of the drawings]

In [1] Experimental Example 1, AMP-2Na (- closed squares -), adenosine (- ▲ -), inosine ^{(- ○ -) 3 H-} 2- deoxyglucose in epidermal keratinocytes for (2-DG) FIG. 3 is a view showing the results of examining the effect of promoting the uptake of. In each figure, the horizontal axis represents the concentration (log M) of each purine base, and the vertical axis represents ³ H-per 1 μg of cell protein.
Incorporation of 2-deoxyglucose (H-DPM (μ
g) / μg protein) (the ratio of the amount of 2-deoxyglucose taken up by cells when the amount is set to 1 for the control).

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61K 31/522 A61K 31/522 31/7076 31/7076 A61P 17/16 A61P 17/16 43/00 105 43/00 105 C07D 473/18 C07D 473/18 473/34 311 473/34 311 C07H 19/20 C07H 19/20 19/207 19/207 (72) Inventor Fumiki Harano 2nd, Takehanakino Honcho, Yamashina-ku, Kyoto, Kyoto Kyoto Yamashina Garden City 113 F-term (reference) 4C057 LL29 LL47 4C083 AC022 AC102 AC122 AC422 AC432 AC472 AC851 AC852 AC901 AC902 AD092 CC02 CC04 CC05 CC37 EE11 EE16 4C086 AA01 AA02 CB07 EA18 MA01 MA17 MA22 MA63 ZA89 ZA89

Claims (12)

[Claims] 1. An agent for promoting sugar uptake in epidermal keratinocytes, comprising a purine base or a salt thereof as an active ingredient. 2. The purine base is selected from the group consisting of adenine, guanine, adenosine monophosphate, adenosine 5'-diphosphate, adenosine 5'-triphosphate, flavin adenine dinucleotide and nicotinamide adenine dinucleotide. The sugar uptake promoter according to claim 1, which is at least one kind. 3. The sugar uptake promoter according to claim 1, wherein the purine base is adenosine monophosphate. 4. An agent 10 for promoting sugar uptake in epidermal keratinocytes
The sugar uptake promoter according to any one of claims 1 to 3, which contains at least 0.1% by weight of a purine base or a salt thereof per 0% by weight. 5. An agent 10 for promoting sugar uptake in epidermal keratinocytes
Purine base or a salt thereof is added in an amount of 0.1 to 1 per 0% by weight.
The sugar uptake promoter according to any one of claims 1 to 4, which is contained in a proportion of 0% by weight. 6. The sugar uptake promoter according to claim 1, wherein the sugar is at least one selected from the group consisting of glucose, arabinose, xylose, mannose, galactose and fructose. 7. A method for promoting sugar uptake in epidermal keratinocytes, which comprises applying a purine base or a salt thereof to the skin. 8. The purine base is selected from the group consisting of adenine, guanine, adenosine monophosphate, adenosine 5'-diphosphate, adenosine 5'-triphosphate, flavin adenine dinucleotide and nicotinamide adenine dinucleotide. The method for promoting sugar uptake according to claim 7, which is at least one kind. 9. The method for promoting sugar uptake according to claim 7, wherein the purine base is adenosine monophosphate. 10. The saccharide according to claim 7, wherein the saccharide is at least one selected from the group consisting of glucose, arabinose, xylose, mannose, galactose and fructose.
10. The method for promoting uptake of sugar according to any one of claims 9 to 9. 11. The sugar uptake according to claim 7, wherein a composition containing at least 0.1% by weight of a purine base or a salt thereof is applied to the skin. How to promote. 12. A purine base or a salt thereof in an amount of 0.1 to 10%.
The method of accelerating sugar uptake according to any one of claims 7 to 11, wherein a composition containing the composition in a ratio of% by weight is applied to the skin.