JP2002340882A - Device and method for measuring dioxins - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a device and a method for easily and efficiently determining quantity of dioxins with high precision in a short time as toxicity data. SOLUTION: In a collection unit 1, a chlorine-containing compound or a bromine-containing compound including the dioxins in a gaseous form and a particle form is collected by means of a filter (a three-dimensional network structure molded body of fiber and an inorganic binder) 7. In a separation unit 11 constructed of an extraction unit 11a and a separation purification unit 11b, mixed chloride compounds and the dioxins are separated from the collected substances. In extraction, a Soxhlet extraction, a superheated steam extraction, an ultrasonic extraction, and a supercritical fluid extraction are available, and in separation and purification, high-performance liquid chromatography or a supercritical fluid chromatography can be used. In a measurement unit 12, activity of the separated component is measured by a bioassay method (enzyme immunoassay method using an immobilized antibody and an enzyme-labeled antibody).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a measuring device and a measuring method useful for simply and quickly quantifying dioxins.

[0002]

2. Description of the Related Art Various chlorine-containing compounds, such as industrial waste and general household waste, are contained in exhaust gas.
Examples include dioxins (generic substances such as polychlorinated dibenzo-para-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs)), polychlorobiphenyls (such as coplanar PCB), chlorophenols, and chlorobenzenes. ing. Among dioxins, tetrachlorodibenzodioxin (T ₄ C
DDs), coplanar P among polychlorobiphenyls
CB is a toxic substance that has a serious effect on living organisms. Chlorine-containing compounds such as chlorophenols and chlorobenzenes are less toxic than dioxins, but dioxins that form dioxins by reactions in incinerators It is known to be a precursor.

In order to objectively grasp the degree of contamination by dioxins and to quantitatively measure dioxins generated from incinerators, Japanese Industrial Standard JIS K 031 is required.
1: 1999 defines a method for measuring dioxins in exhaust gas. This method is performed as follows.

First, in the sampling of dioxins, solids are removed from exhaust gas by a filter, and a plurality of collection bottles (impinger) containing water as an absorbing liquid.
The liquid component is collected by passing through the gas to be measured, and adsorbed with an adsorbent (XAD-2 resin) that adsorbs dioxins,
The gas to be measured that has been subjected to the adsorption treatment is passed through a collection bottle containing diethylene glycol, and the integrated flow rate of the gas to be measured is measured. Each of the collection bins is cooled in a cooling tank.

[0005] Further, to separate dioxins from the collected components, there is an operation of treating the filter with hydrochloric acid and filtering.
The operation of filtering the washing liquid of the collection bottle containing water, the operation of soxhlet extraction using the toluene from the residue and the adsorbent from these filtration, the filtrate by the filtration, and the collection bottle containing diethylene glycol, An operation of shaking extraction from a washing solution or the like using dichloromethane, and an organic solvent layer obtained by these extraction operations are concentrated. These operations are also performed on components adhering to various containers and the flow path of the gas to be measured. Furthermore, the crude extract is separated from the concentrate, concentrated by adding an internal standard, and transferred to hexane. Dioxins are separated from polychlorinated biphenyls (PCBs) by silica gel column chromatography and alumina column chromatography. It is separated or fractionated and concentrated, and an internal standard is added to further concentrate dioxins.

[0006] Further, the components attached to the various containers and the flow path of the gas to be measured are also subjected to the above-mentioned operation. Further, in the determination of dioxins, a gas chromatograph / mass spectrometer (GC / GC) is used.
MS), the content of dioxins is measured by comparing the peak of the internal standard sample with the measured peak for various types of isomers of dioxins.

However, in such a method, the sampling of the sample is complicated and must be strictly performed, and a large number of steps are required to separate or fractionate dioxins. In order to measure with high accuracy, strict process control is required. In addition, operations from sampling to separation or fractionation of dioxins require highly empirical techniques. Further, in a gas chromatograph / mass spectrometer (GC / MS), even if a component (non-toxic isomer) that is not a dioxin is found, if the peak of the internal standard sample matches the measured peak, it is determined as a dioxin. Therefore, dioxins and non-toxic isomers cannot be separated.

Further, since many steps are involved between the sampling of a sample and the analysis by a gas chromatograph / mass spectrometer (GC / MS), the determination of dioxins takes a considerable number of days (for example, 30 days to 2 months). ). Therefore, the operation time is short (for example, 1 day to 1 day).
In small or small incinerators (on the order of a week), it is difficult to monitor dioxins on a daily basis and control combustion conditions and the like.

[0009]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a measuring apparatus and a measuring method which can easily and efficiently quantify dioxins in a short time.

Another object of the present invention is to provide a measuring device and a measuring method which are useful for objectively evaluating dioxins as toxicity data without requiring complicated operations.

Still another object of the present invention is to provide a measuring apparatus and a measuring method capable of quantifying dioxins with high accuracy.

[0012]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies to achieve the above object, and as a result, have found a filter capable of collecting gaseous and particulate dioxins, and a filter which has been collected by the filter. Combining chromatography, which separates dioxins from substances, with bioassay, which measures the activity of separated components containing dioxins, makes it easy and quick to remove dioxins even if the separated components are contaminated with non-toxic isomers. To be able to quantify
The present invention has been completed.

That is, the measuring device of the present invention is a unit for collecting chlorine or bromine-containing compounds containing dioxins with a filter, and separates dioxins from the collected matter collected by the collection unit. And a unit for measuring the activity of the component separated by the separation unit by a bioassay method.
The activity of the components separated by the separation unit may be measured by an immunoassay. In this apparatus, the collecting unit is usually a filter for collecting gaseous and particulate dioxins (for example, a filter composed of a molded article having a three-dimensional network structure composed of fibers and an inorganic binder).
It has. The separation unit may be a single unit, an extraction unit for extracting dioxins from the collected matter (for example, a unit using soxhlet extraction, superheated steam extraction, ultrasonic extraction or supercritical fluid extraction) ) And a separation and purification unit (for example, high performance liquid chromatography or supercritical liquid chromatography) for separating and purifying dioxins from the components extracted by this extraction unit. further,
A measurement unit by a bioassay method such as an immunoassay method is usually an enzyme immunoreaction, for example, a solid phase method using a labeled enzyme or an enzyme-labeled antibody, particularly, a solid-phased antibody capable of binding dioxins and a dioxin-bound antibody. This is a unit that uses a possible enzyme-labeled antibody (a unit that uses the color development concentration of the enzyme-labeled antibody).

[0014] The present invention relates to a dioxin compound for collecting chlorine or bromine-containing compounds containing dioxins with a filter, separating the dioxins from the filter, and measuring the activity of the separated components as toxicity data by a bioassay method. The measurement method is also included.

In the present specification, not only chlorinated dioxins but also brominated dioxins may be collectively referred to as dioxins. Further, the chlorine-containing compound and the bromine-containing compound may be simply referred to as “chlorine-containing compound”.

[0016]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail with reference to the accompanying drawings as necessary. FIG. 1 is a flowchart for explaining the collecting unit of the apparatus of the present invention, and FIG.
FIG. 2 is a process chart for explaining the apparatus and method of the present invention.

The apparatus of the present invention comprises a collection unit (or sampling unit) 1 for collecting chlorine or bromine-containing compounds containing dioxins from a fluid to be measured (such as a gas to be measured generated from an incinerator); Separation unit 11 for separating or fractionating dioxins from collected matter
And a measurement unit 12 for quantifying dioxins from the separated sample containing dioxins. Further, the separation unit 11 includes an extraction unit 11a for extracting dioxins from the collected matter,
A separation and purification unit 11b for separating and purifying dioxins from the components extracted by the extraction unit.

[Collection unit] The collection unit 1
A pipe (glass, for example, borosilicate glass, quartz glass, etc.) 2 through which a fluid to be measured (exhaust gas from an incinerator, etc.) can flow, and a sampling unit 4 detachably mounted in the middle of the pipe. A suction unit (particularly a pump capable of suctioning at a constant speed) 8 attached to a pipe line on the downstream side of the sampling unit for sucking a fluid, and a flow meter 9 for integrating the flow rate of the fluid by the suction unit And

The sampling unit 4 includes a holder 6 which can be attached to and detached from the pipe line 2 by screwing a mounting body 5 or the like. One end of the holder 6 has an opening and the opening is located on the upstream side. The cylindrical filter 7 arranged toward the (inflow side) is held. In the above example, in order to prevent the generation of dioxins in the pipeline 2, the collection unit 4
A cooling unit 3 for cooling the temperature of the fluid to be measured to 120 ° C. or lower is provided on the upstream side. This cooling unit is not always necessary.

In the collecting unit 1, the filter 7 for collecting a chlorine-containing compound or a bromine-containing compound containing dioxins is formed of a three-dimensional network structure having fluid permeability. The form of the filter may be a sheet, a pyramid, a cone, or the like,
One end is opened to allow the fluid to be measured to flow in, and the other end is closed in order to efficiently capture the chlorine-containing compound or the bromine-containing compound in the flowing fluid to be measured. Body). This molded body is
It contains fibers and a binder (especially an inorganic binder) for bonding the fibers to form an amorphous network.

The fibers are fibers which are substantially inactive (do not react) with chlorine-containing compounds (chlorinated organic compounds) or bromine-containing compounds such as dioxins and their precursors, polychlorobiphenyls, For example, fibrous activated carbon (such as pitch-based fibrous activated carbon), carbon fiber (such as pitch-based carbon fiber), glass fiber, alumina fiber (such as activated alumina fiber), inorganic fiber such as silica fiber, and fluororesin fiber (such as polytetrafluorocarbon) Ethylene fiber)
And other organic fibers. These fibers can be used alone or in combination of two or more. The fiber diameter and specific surface area of these fibers are not particularly limited, and the average fiber diameter is, for example, 1 to 50 μm, preferably 1 to 30 μm,
More preferably, it may be about 1 to 20 μm. The aspect ratio (length / diameter) of the fiber may be in a range where the pressure loss does not excessively increase with the passage of the fluid, and is, for example, 10,000 or less (for example, about 1000 to 10000), preferably about 1500 to 5000. It may be.

The binder may be an organic binder (eg, a non-aromatic resin containing no halogen atom such as a chlorine atom such as a cellulose derivative), but is preferably an inorganic binder. The inorganic binder functions as a binder capable of holding the fiber group or the fiber aggregate in a predetermined shape, and is substantially inert to chlorine-containing compounds (chlorinated organic compounds) such as dioxins or bromine-containing compounds. I just need. Examples of such an inorganic binder include, for example,
Examples include alumina (eg, activated alumina), zeolite (eg, synthetic zeolite), silicon dioxide (silica), acid clay, apatite, and the like. These binders can be used alone or in combination of two or more. The form of the binder is not particularly limited, and may be, for example, a particulate or fibrous form.

The binder (particularly, an inorganic binder) preferably has a tar-adsorbing property. When such a binder is used, chlorine or bromine-containing compounds such as dioxins contained in tar can be effectively and reliably captured. Examples of such a binder include tar-adsorbing inorganic binders such as alumina (particularly activated alumina), zeolite, and silicon dioxide.

The ratio of the fiber and the binder is, for example, the former / the latter = 10/90 to 90/10 (weight ratio), preferably 20/80 to 80/20 (weight ratio), more preferably 30/90. It can be appropriately selected from the range of about 70 to 70/30 (weight ratio).

The bulk density of the molded article composed of the fibers and the binder impairs the ability to capture chlorine-containing compounds or bromine-containing compounds such as dioxins, increases the pressure loss, and impairs the separation efficiency in the subsequent separation step. Can be selected within a range not present, for example, 0.1 to 1 g / cm ³ , preferably 0.2 to 0.8.
g / cm ³ , more preferably 0.3 to 0.7 g / cm ³
It is about. In addition, the bulk density of a molded object can be adjusted by the ratio of a fiber and a binder.

A specific molded article is composed of activated alumina fibers as fibers and particulate activated alumina as an inorganic binder, and has a bulk density of 0.3 to 0.7 g.
/ Cm ³ (for example, 0.4 to 0.6 g / cm ³ ). The size of the filter is, for example, 50 to 150 mm in length and 12 to 3 in outer diameter on the opening side.
5 mm, outer diameter 10-30 mm on the closed end side, thickness 1-1
It may be about 0 mm.

The filter formed of such a molded product is formed, for example, by molding a dispersion containing the fibers and the binder into a cylindrical shape having one end closed by a conventional method, for example, a wet die molding method. Then, the obtained preformed body can be obtained by heat treatment (for example, sintering). Heat treatment or sintering temperature can be selected according to the type of fiber and binder,
For example, when alumina is used as at least one of the fiber and the binder, the temperature may be a temperature at which the alumina can be activated, for example, about 150 to 170 ° C.

The molded body thus obtained is further immersed in a binder-containing dispersion and subjected to a drying treatment (for example, a heat treatment at a temperature of about 150 to 700 ° C.). May be adjusted or increased. When the content of the binder is increased, the chlorine-containing compound or the bromine-containing compound can be efficiently trapped even in the fluid (gas) to be measured containing a large amount of unburned hydrocarbons or carbon monoxide.

In the gas to be measured containing a large amount of carbon compounds such as unburned hydrocarbons and carbon monoxide, tar derived from the carbon compounds is easily generated. In particular, when a general filter is used, the concentration of carbon monoxide in the gas to be measured is reduced to 15%.
If it is 0 ppm or more, the tar component easily passes through the filter. However, in a molded article in which the fiber and a binder having a tar adsorption ability are combined, the concentration of carbon monoxide in the gas to be measured is high (for example, a concentration exceeding 500 ppm).
Even in this case, the tar component can be effectively captured.

A preferred filter is a filter capable of collecting gaseous and particulate dioxins simultaneously or in a single step. Using such a filter can greatly improve the sampling efficiency of a sample, and makes the apparatus simple and compact. Can be Such a filter, as described above,
It has a three-dimensional network structure (or an amorphous three-dimensional network structure) composed of fibers and a binder (particularly, an inorganic binder).

The fluid to be measured is usually sucked at a constant speed, and dioxins in the fluid to be measured (gas to be measured such as exhaust gas) are collected by a filter (such as a cylindrical filter paper). The temperature of the fluid to be measured (gas to be measured) is not particularly limited, but the temperature of the gas to be measured from the incinerator is usually 120 ° C or lower (for example, about 10 to 100 ° C, preferably about 20 to 80 ° C).
It is.

When the amount of dust in the gas to be measured is large, a filter for collecting solids may be provided in advance on the upstream side (for example, on the upstream side of the sampling unit). If the gas to be measured has a large amount of moisture, a moisture removing unit such as a drain bin may be provided in advance on the upstream side of the filter.

When a filter capable of trapping the gaseous and particulate dioxins is used, the amount of sample collected can be reduced.
It can be controlled by a simple operation of adjusting the sampling time according to the concentration of dioxins suitable for a bioassay such as an immunoassay.

[Separation unit] In order to separate dioxins from the collected matter collected by the collecting unit 1, in the separation unit 11, dioxins are extracted from the collected matter by an extraction operation in an extraction unit 11a and analyzed. The dioxins are separated and purified from the extracted components in the separation / purification unit 11b in order to separate and purify the dioxins by fractionating with components that impair the accuracy (such as chlorine-containing compounds such as polychlorobiphenyls).

In the extraction unit 11a, various extraction operations can be used. High-speed solvent extraction such as Soxhlet extraction, extraction with superheated steam, ultrasonic extraction in the presence of an extraction solvent while irradiating ultrasonic waves, supercritical Supercritical Fluid Extraction Using Fluid as Extraction Solvent
d Extraction) and the like. By using such an extraction, dioxins can be efficiently extracted into an extraction solvent within a short time, and the time can be significantly reduced. In superheated steam extraction, selective extraction is possible by utilizing the activity of superheated steam. Furthermore, when supercritical fluid extraction is used, dioxins can be more efficiently and quickly (for example, 5 minutes to 2 hours) with a small amount of fluid without using an organic solvent.
In particular, it can be extracted within 10 minutes to 1 hour.

The filter 7, which has collected dioxins in the collection unit 1, is mounted on the separation unit 11,
If Soxhlet extraction or supercritical fluid extraction is used, dioxins can be automatically extracted.

The extraction of dioxins includes superheated steam, aromatic hydrocarbons (toluene, xylene, etc.), alicyclic hydrocarbons (cyclohexane, etc.), halogenated hydrocarbons (dichloromethane, dichloroethane, etc.). Available.

In the supercritical extraction, various supercritical fluids (eg, a fluorine-containing solvent, nitric oxide, carbon dioxide, etc.) can be used. Modifiers, for example, alcohols such as methanol, trifluoroacetic acid, proton acids such as sulfuric acid and Lewis acids such as boron fluoride, trimethylsilylating agents, ion forming agents and the like may be added to these supercritical fluids. Good. These modifiers can be used alone or in combination of two or more.

The amount of the extraction solvent can be appropriately selected. For example, for a filter having a filter weight of 5 to 20 g, 50 to 750 ml (for example, 100 to 5
00 ml), and may be about 3 to 100 ml (for example, 3 to 50 ml) for supercritical fluid extraction. The extraction temperature can be appropriately selected. For example, in the case of solvent extraction, it can be selected from the range of room temperature to the reflux temperature of the solvent, and in the case of supercritical fluid extraction, extraction can be performed at room temperature to about 200 ° C. In the ultrasonic extraction, for example, the frequency 2
It may be about 0 kHz to 5 MHz.

Various column chromatography and the like can be used in the separation / purification unit 11b. To separate and purify the mixed chlorinated products and dioxins from the components extracted from the extraction unit 11a, high-performance liquid chromatography ( It is advantageous to utilize HPLC), Supercritical Fluid Chromatography. For high performance liquid chromatography, various mobile phases (developing solvents) such as aromatic hydrocarbons (toluene, xylene, etc.), alicyclic hydrocarbons (cyclohexane, etc.), halogenated hydrocarbons (dichloromethane,
Dichloroethane), and the like. For supercritical fluid chromatography, the supercritical fluid (carbon dioxide, etc.)
Can be used as a mobile phase.

When such chromatography is used, not only separation and purification but also concentration can be performed.
It is advantageous for analysis by a bioassay such as an immunoassay. Furthermore, purification time can be shortened and automation is possible, and speeding up and simplification are possible. In addition, when the cylindrical or cylindrical filter having one end opened is used, a solvent or a fluid can be introduced from the opening of the filter, and the dioxins can be extracted by passing through the filter. Efficiency can be improved.

[Measurement Unit] In the measurement unit 12,
The activity of the component (sample) separated by the separation unit 11 is quantitatively analyzed by a bioassay such as an immunoassay. The type of bioassay (immunoassay) is not particularly limited, and a liquid phase method for measuring an antigen-antibody complex in a liquid phase may be used. However, usually, a solid phase using an antibody bound to a carrier (solid) is used. The phase method is preferred. The carrier (insoluble carrier) for immobilizing the antibody may be a plate, a stick, a reaction vessel wall (for example, a test tube), latex particles (for example, polystyrene particles, etc.). In the bioassay, a measurement sample can be prepared using an appropriate solvent, for example, water, dimethyl sulfoxide (DMSO), or the like.

In the solid phase method, an agglutination method in which the degree of agglutination is measured visually or by a turbidimeter using an agglutination reaction in which solid particles to which antibodies are bound via an antigen may be used,
A labeling method using an antibody (labeled antibody or labeling enzyme) that can bind to the substance to be measured and is bound to a label is preferable. In addition, in the labeling method, enzyme immunoassay (ELISA, enzyme-linked immunosorbent) is more effective than radioimmunoassay.
assay) is advantageous. Examples of the label include a radioisotope (radio isotope), a fluorescent labeling agent (such as fluorescein isothiocyanate), a chemiluminescent labeling agent (such as an N-methylacridinium ester derivative, an isoluminol derivative), and an enzyme (such as peroxidase). ) Can be used.

A preferred bioassay is an enzyme immunoassay using a solid-phase method in which a solid-phased antibody capable of binding dioxins and an enzyme-labeled antibody capable of binding dioxins are used in combination. As a typical solid phase method, a sandwich method can be exemplified. In this method, a sample (a sample containing dioxins as an antigen) is added to an antibody (an immobilized antibody or an immobilized antibody), and the antigen is bound to the immobilized antibody by an antigen-antibody reaction. Remove antigen. Then, after adding an enzyme-labeled antibody capable of binding to the antigen (a secondary antibody labeled with an enzyme) to cause an antigen-antibody reaction, the free enzyme-labeled antibody is removed by washing or the like, and a substrate is added. An enzymatic reaction is performed, color is developed based on the amount of enzyme bound to the secondary antibody, and the concentration of the antigen is measured based on the color development concentration.

In this method, a test solution containing dioxins and a labeling enzyme are added to a test tube in which an antibody is immobilized on the inner wall to allow competitive binding to the antibody, and unreacted substances are washed (with water or washing solution). ), Add reagents that react with the labeling enzyme (substrate reagent and color-forming reagent), leave for a predetermined time to develop color, add stop solution, and measure the color density with a photoelectric colorimeter. The concentration of dioxins can be measured from the difference from the control. The concentration of dioxins can be determined based on a calibration curve created in advance.

By using such a bioassay, the activity of the whole sample containing dioxins can be evaluated as toxicity data. Therefore, even if the sample contains dioxins and non-toxic isomers, the amount of dioxins can be evaluated as a practical toxicity value (toxicity data) and quantified as toxicity data. Furthermore, since the bioassay method is used, dioxins can be measured with high accuracy even at a low concentration (for example, about 1 pg).

In the present invention, a step or unit for collecting a chlorine-containing compound or a bromine-containing compound containing dioxins with a filter (collection unit) and a step or unit for separating dioxins from the filter (separation unit) Since the method combines the step (or measurement unit) of measuring the activity of the separated component by a bioassay method, dioxins can be quantified inexpensively and in a short time, for example, within one week (in particular, about three days). . In particular, since the activity of a separated component containing dioxins is measured by a bioassay method, even if dioxins and non-toxic isomers (contaminant components) that are difficult to separate from dioxins coexist. And components containing dioxins can be quantified as practical toxicity data. Therefore, the present invention can be used for various applications in which generation and presence of dioxins are regarded as problems.

Incidentally, the separation step or the separation unit comprises:
It does not need to be composed of an extraction unit and a separation and purification unit, and may be composed of a single step or unit. For example, extraction and separation / purification may be combined and performed continuously or automatically in one step or unit. The step of attaching the filter obtained in the collection step to the separation unit, the separation step by the separation unit, and the measurement step by the measurement unit may be performed continuously or automatically by using a robot if necessary. . The components separated in the separation step may be concentrated and provided to the measurement step if necessary.

Although the present invention can be applied to various fluids (liquids such as drinking water and industrial waste liquid), it is preferably useful for measuring dioxins in exhaust gas generated from a combustion furnace or an incinerator.

[0050]

According to the present invention, the step of collecting a sample from the fluid to be measured, the step of separating dioxins from the collected substance, and the step of measuring the toxicity of the separated component are combined. Can be simply and efficiently determined within a short time. Further, dioxins can be objectively evaluated as toxicity data without requiring complicated operations. Furthermore, since the bioassay method is used, dioxins can be quantified with high accuracy even when nontoxic isomers and the like are mixed.

[Brief description of the drawings]

FIG. 1 is a flowchart for explaining a collecting unit of the apparatus of the present invention.

FIG. 2 is a process chart for explaining an apparatus and a method of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Collection unit 2 ... Pipe line 4 ... Collection unit 6 ... Holder 7 ... Cylindrical filter 11 ... Separation unit 11a ... Extraction unit 11b ... Separation / purification unit 12 ... Measurement unit

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) G01N 1/02 G01N 1/02 D 1/22 1/22 D 30/04 30/04 A 30/06 30 / 06 Z 30/88 30/88 C 33/53 33/53 S // B01D 39/14 B01D 39/14 M 39/16 39/16 A 39/20 39/20 BC (72) Inventor Masayoshi Yoshioka Ki 1-4-2, Hirano-cho, Chuo-ku, Osaka-shi F-term in Osaka Gas Co., Ltd. (reference) 2G052 AA02 AB22 AC25 AD03 AD26 AD46 BA03 BA22 CA02 CA12 EA03 EA08 EB13 ED03 GA30 HA15 JA03 JA07 JA09 4D019 AA01 BA03 BA04 BA05 BA13 BA16 BB03 BB07 BC05 DA02 4D056 AB19 BA13 BA16 BA20 CA01 CA17 CA21 CA23 CA26 CA40

Claims (9)

[Claims] 1. A unit for collecting a chlorine or bromine-containing compound containing dioxins with a filter, a unit for separating dioxins from the collected matter collected by the collection unit, and a separation unit. A unit for measuring the activity of the components separated by the above method by a bioassay method. 2. The collecting unit according to claim 1, wherein the collecting unit includes a filter for collecting dioxins in gaseous form and particulate form.
The measuring device as described. 3. The measuring apparatus according to claim 1, wherein the filter is formed of a three-dimensional network structure formed of fibers and an inorganic binder. 4. The separation unit comprises an extraction unit for extracting dioxins from the collected matter, and a separation and purification unit for separating and purifying dioxins from components extracted by the extraction unit. The measuring device according to claim 1. 5. The measuring device according to claim 4, wherein the extraction unit is a unit that utilizes Soxhlet extraction, superheated steam extraction, ultrasonic extraction, or supercritical fluid extraction. 6. The measuring apparatus according to claim 4, wherein the separation and purification unit is constituted by high performance liquid chromatography or supercritical liquid chromatography. 7. The measuring apparatus according to claim 1, wherein the measuring unit by the bioassay method is a unit utilizing an enzyme immunoreaction. 8. The measurement device according to claim 1, wherein the measurement unit by the bioassay method is a unit utilizing an immobilized antibody capable of binding dioxins and an enzyme-labeled antibody capable of binding dioxins. 9. A method for collecting dioxin-containing chlorine or bromine-containing compounds by a filter, separating the dioxins from the filter, and measuring the activity of the separated components as toxicity data by a bioassay method. Method.