JP2002253213A - Soil disease preventing microorganism, method for using the same and culture soil containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new kind of microorganism having soil disease (preventing) effect even in the presence of an agrochemical such as thiuram, metalaxyl, benomyl or captan, to provide culture soil containing the microorgan ism, and to provide a raising seedling method. SOLUTION: The objective culture soil is obtained by adding a culture microorganismal liquid of actiomyces Streptomyces rochei ss. rochei A13 to field soil or raising seedling culture soil. The other objective raising seedling method involves sowing the above culture soil with crop seeds.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a new species of actinomycete Streptomyces roche ess roscheri A13, which remarkably prevents soil diseases such as wilt of plants caused by phytopathogenic fungus Fusarium and the like. The present invention relates to a method for controlling soil diseases of plants, characterized in that Seth Roche es Roche A13 is resistant to pesticides thiuram, metalaxyl, benomyl and captan, which are used for measures against take-all disease such as vegetable seeds.

[0002]

2. Description of the Related Art There are many soil diseases of plants, which are difficult to eradicate due to the durability of the pathogenic bacteria, and fumigating the soil with methyl bromide or the like is regarded as an effective control method. But,
These fumigants are problematic in that they cause toxicity and cause global warming, and it has been reported that the use of methyl bromide was completely banned in 2005. In addition, at present, even if the soil is disinfected with these fumigants, the occurrence of soil diseases cannot be suppressed due to the weather, the soil temperature, and the like. Therefore,
In consideration of the environment, methods such as steam sterilization and solar heat sterilization of the soil are taken, but these kill not only plant pathogens but also useful microorganisms that are symbiotic with plants necessary for plant growth, Destroying the soil ecosystem. To cope with such a problem, there is a method for reducing soil diseases using effective microorganisms. For example, Japanese Patent Application Laid-Open No. 05-168348 discloses that Fusarium,
Using filamentous fungi like Trichoderma, bacteria like Pseudomonas, actinomycetes like Streptomyces,
It is described that the addition of these antagonistic antibacterial agents to the seedling culture medium reduces the occurrence of soil diseases.

On the other hand, conventionally, after sowing seeds such as vegetables,
A large number of damping-off diseases occur, and many pesticides such as thiuram, metalaxyl, benomyl, and captan are used to control the damping-off. Some seeds are coated with two kinds of pesticides, such as spinach seeds, thiuram and metalaxyl, and rarely, three kinds of pesticides, thiuram, benomyl, and captan. Therefore, in order to suppress the activity of the soil disease pathogen by the effective microorganism, there is a problem that the disease control effect cannot be sufficiently exhibited unless the effective microorganism used is resistant to these pesticides.

[0004]

SUMMARY OF THE INVENTION The present invention provides a thiuram,
To provide a novel microorganism capable of sufficiently exerting the effect of controlling soil diseases such as wilt even in the presence of pesticides such as metalaxyl, benomyl, and captan, and a seedling containing the microorganism and a seedling raising method for reducing soil diseases of crops. With the goal.

[0005]

As a method for solving these problems of the conventional control technology, the present inventor selects a large number of actinomycetes exhibiting a soil disease control effect from coffee grounds compost, and furthermore selects them. The actinomycete Streptomyces roche S. es Roche A13 showing a strong disease control effect was isolated from Actinomycete. A culture solution of this actinomycete was added to and mixed with a small amount of seedling cultivation soil compared to the field soil, and a seedling raising method for controlling soil diseases by transplanting the seedlings into the field soil was established, thereby completing the present invention. Was.

That is, the present invention provides (1) a microorganism Streptomyces roche es es roche A13 having a soil disease control function, and (2) a streptomyces rosacea.
A soil disease reducing cultivation medium characterized by containing Roche SS Roche A13 in an amount of 10 ⁴ to 10 ¹⁰ CFU per gram of dry soil; (3) using Roche A13 in soil 1
seedling method comprising sowing seeds of a crop on a culture medium containing 10 ⁴ to 10 ¹⁰ CFU per g dry soil,
(4) The method according to (3), wherein the seed is a seed coated and / or coated with at least one or more pesticides selected from thiuram, metalaxyl, benomyl, and captan.

[0007] Streptomyces roche es es roche A13 (hereinafter abbreviated as A13 strain) referred to in the present invention has the following morphological properties, chemical classification, culture properties, and physiological properties. (1) Morphological properties The findings were cultured on a starch-inorganic salt agar medium and glycerol-asparagine agar medium at 28 ° C. for 20 days and observed with an optical microscope and an electron microscope. Well-branched mycelium (approximately 0.4 micrometer) to abundant aerial hyphae (approximately 0.6 micrometer)
, The tip of which is a loose spiral of 4-5 times. It forms about 10-20 linked spores (0.6-0.8 micrometer x 0.8 micrometer-1.2 micrometer) and its surface is smooth.

(2) Chemical Classification (Diaminopimelic Acid) Methods such as Stanne Ck [Appl. Microbio
l. 28; 226 to 231 (1974)], LL-type diaminopimelic acid was detected,
No meso-type was detected. (3) Culturing properties The results of culturing on various media at 28 ° C. for 20 days and observations are as shown in Table 1. (Color display is Color h
armony manual 4th edition 1958 Co
tainer Corporation of Am
erica. )

(4) Physiological properties Growth temperature range: 10 ° C. to 43 ° C. Production of melanin-like pigment: on tyrosine agar: negative
On peptone yeast extract / iron agar medium: negative Use of carbon source: D-xylose, L-arabinose, D-glucose, D-fructose, sucrose, raffinose, L-rhamnose, inositol, D
-Utilize mannitol.

The culture characteristics of the A13 strain are shown in Table 1.

[Table 1]

FIG. 1 is a chart showing a gas chromatographic analysis of the fatty acid composition of the A13 strain. The A13 strain of the present invention was deposited at the National Institute of Advanced Industrial Science and Technology, Biotechnology and Industrial Technology Research Institute, located at 1-3 1-3 Higashi, Tsukuba, Ibaraki, Japan. The deposit date is January 18, 2001, and the deposit number is FERM P-18169. A of the present invention
In order to control the soil disease or reduce the occurrence of the disease with the 13 strains, a solution containing the A13 strain may be added to the field soil or the seedling culture. At this time, 10 ⁴ -10
^{10 5} CFU, more preferably 10 ⁵ to 10
^It is better to add so that it becomes ⁸ CFU. When the amount of the A13 strain is less than the lower limit, the disease control effect becomes unstable. When the amount is more than the upper limit, not only the cost increases but also the labor for mixing increases.

The A13 strain of the present invention comprises a yeast extract / malt extract medium (4.0 g of yeast extract in 1000 ml of water,
(Including 10.0 g of malt extract and 4.0 g of glucose) by liquid culture at 28 ° C. for 7 days.
A culture solution having a bacterial count of 10 ⁶ to 10 ⁸ CFU per strain is obtained and can be used as a solution containing A13 strain. In addition,
In the present invention, the number of bacteria (CFU) in the culture solution of the A13 strain and other actinomycetes and in the soil were determined by a dilution plate method using an albumin agar medium. In other words, “Soil microbial experiment method” (edited by Soil Microorganisms Research Society, Yokendo 199)
2) According to the method for counting, separating and identifying soil bacteria on pages 15 to 18 and the method for counting, separating and identifying soil actinomycetes on pages 55 to 61, albumin agar medium (egg albumin 0.25 g, glucose 1 g in 1000 ml of water) , K ₂ HPO ₄ ,
After culturing at 28 ° C. for 7 days using 0.2 g of MgSO ₄ .7H ₂ O, traces of Fe ₂ (SO ₄ ) ₂ , and 15 g of agar, soil microbial experiment method, the colonies that appeared were counted. In addition, the A13 strain of the present invention has an antagonistic effect in the confrontation culture method against Fusarium, Rhizoctonia, and Pycium that are pathogenic bacteria of plant soil diseases. Therefore, the use of the A13 strain of the present invention , Soil disease caused by pathogenic bacteria such as Rhizoctonia and Picium can be controlled.

The field soil referred to in the present invention is the soil of a cultivation field that produces agricultural crops, and includes, for example, ando soil, brown forest soil, gray plateau soil, grey soil, brown lowland soil, gray lowland soil, and the like. . Seedling cultivation soil is a soil for growing seedlings to be transplanted to field soil, and the above-mentioned soil seeds may be used as they are, or may be sterilized and granulated, or may be inorganic materials such as vermiculite, perlite, and zeolite. A mixture of artificial cultivation in which organic materials such as peat moss, coconut shell fiber, humus and the like are mixed may be used. It is also possible to mix and use artificial soil and soil used as field soil. Many crops such as spinach, leek, lettuce, cabbage, watermelon, tomato, and cucumber grow seedlings using seedling cultivation soil.

The method of raising seedlings using the A13 strain of the present invention
Seeds of the crop are sown on the seedling culture soil to which the solution containing the A13 strain has been added, and after the seeds have been grown, the seedlings are planted in a cultivation field. At this time, the number of bacteria of the A13 strain in the seedling cultivation soil was 1 per gram of dry soil.
0 ⁴ to 10 ¹⁰ CFU, more preferably 1
By adding so as to be 0 ⁵ to 10 ⁸ CFU, soil diseases can be controlled. The seedling raising methods include ground floor seedlings that do not use seedling containers, pot seedlings that use plastic or unglazed pots, paper pot seedlings that use paper-connected pots, and cell seedlings that use plastic cell trays. Then, the A13 strain is added to the seedling cultivation medium described above, and if necessary, fertilizers and pH adjusters are added and mixed, and then the seeds of the crop are sown, and the seeds are irrigated and optionally heated to promote germination. I do. After germination, normal temperature and water management are performed, and seedlings of an appropriate size are grown.

The A13 strain of the present invention has resistance to thiuram, metalaxyl, benomyl and captan applied to the seeds of vegetables and the like for controlling damping-off and the like. Even when seeds such as vegetables to which A13 is applied are sown on field soil or seedling cultivation soil to which the A13 strain of the present invention has been added, soil diseases can be controlled without any influence. The thiuram referred to in the present invention is represented by bis (dimethylthiocarbamoyl) disulfide by the chemical formula _{C 6 H 12 N 2 S 4} . Further, metalaxyl referred to in the present invention is represented by C ₁₅ H ₂₁ NO ₄ . Further, the benomyl referred to in the present invention, represented by the chemical formula C ₁₄ H ₁₈ N ₄ O ₃ methyl-1- (butylcarbamoyl) -2-benzimidazole carbamate. In addition, the captan referred to in the present invention is N-trichloromethylthiotetrahydrophthalimide and represented by the chemical formula C ₉ H ₈ Cl ₃ NO ₂ S. These are used as a main component of agricultural chemicals for disinfecting seeds of plants and spraying on foliage, etc., for controlling plant damping-off.

[0016]

DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment using a spinach seedling raising system according to the present invention will be described below.

[Example 1] Preparation of disease-causing soil: Fusarium oxysporum (MAFF 1) as a spinach wilt pathogen
03059) was mixed with spinach cultivation soil to make 10 ⁵ per 1 g of cultivation soil to prepare a disease-causing soil.

[0017]

Example 2 Isolation of A13 strain: The A13 strain of the present invention was selected by the following method. “Soil microbial experiment method” (edited by Soil Microorganisms Research Association, Yokendo 1992) from coffee grounds compost (manufactured by Green Products Co., Ltd.) 15
Methods for counting, separating, and identifying soil bacteria on pages 18 to 55
According to the method for counting, separating and identifying soil actinomycetes on page 61, actinomycetes were isolated by the dilution plate method using the aforementioned albumin agar medium (Soil microbial experiment method, page 380).

That is, 30 g of coffee grounds compost is added to 270
The mixture was added to ml of sterile water and shaken with a reciprocating shaker for 10 minutes. The one after shaking was used as a primary diluent. After this was stirred well, 10 ml was taken with a micropipette equipped with a sterilized tip and placed in 90 ml of sterile water. This was used as a secondary diluent. While stirring the secondary diluent, 10
ml was added to 90 ml of the next diluent to obtain a tertiary diluent.
The same procedure was followed until the sixth dilution. 1 ml of 6th dilution
Pour into albumin agar medium dispensed into 9 cm Petri dish,
It was spread with a sterilized glass rod and cultured at 28 ° C. for 7 days. 7
The actinomycetes grown on the day were individually transferred to slants prepared on albumin agar medium and isolated. For each isolated actinomycete, take one platinum loop from the slant into 3 ml of sterile water, and again
Pure separation was performed by the dilution plate method.

Each of the actinomycetes isolated from the coffee grounds compost was isolated using a yeast extract / malt extract medium (containing 4.0 g of yeast extract, 10.0 g of malt extract, and 4.0 g of glucose in 1000 ml of water). Liquid culture at 28 ° C for 7 days,
Using the obtained cultures of actinomycetes, the effect of suppressing the onset of spinach wilt was investigated. The disease-producing soil prepared in Example 1 was mixed with a culture solution of each actinomycete in an amount of 50 ml per liter of the disease-producing soil. In order to promote germination, spinach ("Okame" manufactured by Takii Seedling Co., Ltd.) soaked in running water for 12 hours was sowed, and using a planter of about 3 L,
Cultivation was carried out in an artificial weather vessel at 30 ° C. for 12 hours assuming daytime and at 25 ° C. for 12 hours as night time. The number of cultivated strains was two planters per test plot, for a total of 30 strains.

Three weeks after the seeding, the wilt of spinach in each planter was observed, and the disease rate was calculated from the number of diseased strains with respect to the number of seeds in each test plot. Was determined. As a result, A
The actinomycetes of the four strains 10, 10, A13, A17 and A17 had a disease rate of 50% or less of the disease rate in the untreated plot, and it was determined that the disease control effect was high. Among them, actinomycetes designated as A13 showed a remarkable disease control effect. Table 2 shows the results of the disease control effect of each actinomycete on spinach wilt.

[0021]

[Table 2]

[0022]

Example 3 Deterrence of A13 strain against soil-borne pathogenic fungi: One platinum loop of A13 strain from slant, φ9 cm
The solution was spread on an albumin agar medium using a petri dish and cultured at 28 ° C. for 4 days. Fusarium oxysporum (MAFF 10305) previously cultured on a potato dextrose agar medium (manufactured by Eiken Chemical Co., Ltd.)
9), cornmeal agar (Difco Labor)
atories), and the leading edge of the microflora of Rhizoctonia solani (MAFF 305241) or Pysium aphanidel mutam (IFO 7030) was extracted with a φ5 mm cork borer.
Each strain was placed 30 mm apart on a plate medium on which thirteen strains grew. Then, when the cells were cultured at 28 ° C., an inhibition zone was formed, and the width was measured. Table 3 shows the results. A1
It was confirmed that the three strains strongly antagonized Fusarium, Rhizoctonia, and Pycium which are pathogenic bacteria of plant soil disease. In addition, when the A13 strain of the present invention was not applied to the plate medium, no inhibition zone was formed.

[0023]

[Table 3]

[0024]

Example 4 Control of spinach wilt by adding A13 strain to seedling cultivation soil: Actinomycete Streptomyces roche es ss roche A13 was transformed with the yeast extract described above.
Using a malt extract medium, liquid culture was performed at 28 ° C. for 7 days, and the disease control effect when the obtained culture solution was added to the seedling culture medium was tested. To 1 L of a commercially available seedling cultivation soil [Nitten EE cultivation soil (special cultivation soil for spinach), manufactured by Nippon Sugar Beet Sugar Co., Ltd.], 10 m of a culture solution of a culture of the actinomycetes Streptomyces roche es es roche A13 was added.
1, 50 ml, 100 ml and 200 ml were added. At this time, the actinomycetes Streptomyces roche S. S. roche A per 1 g dry soil of the seedling culture soil
The number of bacteria of 13 was 1.2 × 10 ^{6 to} 2.4 × 10 ⁷ CFU. Spinach ("Okame", manufactured by Takii Seedling Co., Ltd.) soaked in running water for 12 hours was sown to promote germination, and the seedlings were grown in an artificial weather device under the conditions described in Example 2 for 14 days. For the seedling raising, a connected paper pot ("CP303" manufactured by Nippon Sugar Beet Co., Ltd.) and a seedling tray for paddy rice were used. Spinach seedlings grown for 14 days were transplanted and cultivated in the disease-producing soil prepared in Example 1. Cultivation is 3L
Was performed under the same conditions as in Example 2.
Three weeks after the transplantation, wilt symptoms of spinach in each planter were observed, and the disease rate was calculated from the number of diseased strains relative to the number of transplanted strains, and those with a small disease rate were determined to have a high disease control effect.

As a result, the culture solution of the actinomycete Streptomyces roche es ss roche A13 was applied to 1.2 × 10 ^{6 to} 2.4 × 10 ⁷ C per 1 g dry soil of the seedling culture.
By adding FU, a disease control effect was observed. Table 4 shows the results.

[Table 4]

[0026]

Example 5 Control effect of A13 strain on spinach wilt in the presence of thiuram: Actinomyces streptomyces roche es es roche A13 was liquid cultured at 28 ° C. for 7 days using a yeast extract / malt extract medium. . The obtained bacterial culture solution was applied to a commercially available seedling cultivation soil [Nitten EE cultivation soil (Nitten EE cultivation soil) (manufactured exclusively for rain spinach) manufactured by Nippon Sugar Beet Co., Ltd.], and the number of bacteria per 1 g of dry soil was 6.0 × 1.
0 was added so as to be in ^6. As for the thiuram presence condition, the thiuram applied to the surface of spinach seeds (“Okame” manufactured by Takii Seed Co., Ltd.) was seeded on a seedling culture medium to which the A13 strain was added without washing and removal. In the absence of thiuram, thiuram applied to the surface of spinach seeds was removed by washing with running water for 12 hours, and then sown on seedling culture soil to which A13 strain was added. The spinach seedlings grown for 14 days were transplanted into the disease-producing soil prepared in Example 1, and cultivated under the same conditions as in Example 2 using a 3 L planter. Three weeks after the transplantation, wilt symptoms of spinach in each planter were observed, and the disease rate was calculated from the number of diseased strains relative to the number of transplanted strains, and those with a small disease rate were determined to have a high disease control effect.

As a result, the actinomycetes Streptomyces roche S. roscheri A13 showed an effect of controlling spinach wilt disease even in the presence of thiuram.
Table 5 shows the results. Therefore, even when seeds such as vegetables to which thiuram is applied are sown on field soil or seedling cultivation soil to which actinomycetes Streptomyces roche es es roche A13 has been added, it is possible to control soil diseases without any influence. can do.

[0028]

[Table 5]

[0029]

[Comparative Example 1] Comparison of the control effect of other actinomycetes on spinach wilt in the presence of thiuram: For actinomycetes A10, A11 and A17 which were confirmed to have the effect of controlling spinach wilt in Example 2 And the control effect of spinach wilt on thiuram presence and absence conditions. In addition, actinomycete A10 was identified as Streptomyces achromogenes, actinomycete A11 was identified as Streptomyces hygroscopicus, and actinomycete A17 was identified as Streptomyces limosus.

Actinomycete A10, Actinomycete A11, Actinomycete A1
The three strains 7 were each liquid-cultured at 28 ° C. for 7 days using a yeast extract / malt extract medium. The obtained bacterial culture solution was applied to a commercially available seedling cultivation soil [Nitten EE cultivation soil (Nitten EE cultivation soil) (manufactured exclusively for rain-repelling spinach) manufactured by Nippon Sugar Beet Co., Ltd.], and the number of bacteria per gram dry soil was 6.0 × 1
0 was added so as to be in ^6. Then, in the same manner as in Example 5, a test for confirming the effect of controlling the disease of spinach wilt under the conditions in which thiuram was present and in the absence of thiuram was performed. As a result, actinomycetes A10, A11, and A17
As compared with the thiuram-free condition, the disease-causing strain rate increased and the disease control effect decreased in the thiuram-free condition. Table 6 shows the results.

[0031]

[Table 6]

[0032]

Example 6 Resistance of A13 strain to pesticides: Actinomyces streptomyces roche ess roche A13
The resistance of spinach seeds to pesticides was investigated by a culture test. "Okame" manufactured by Takii Seed Co., Ltd. as spinach seeds coated with thiuram on the surface
As spinach seeds coated with two kinds of pesticides, thiuram and metalaxyl, Watanabe Seed Co., Ltd.
"Tonic" manufactured by Sakata Seed Co., Ltd. was used as spinach seeds coated with three pesticides, thiuram, benomyl and captan. For each of these spinach seeds, 200 ml of distilled water was added to 200 grains, and the mixture was stirred with a stirrer for 24 hours to prepare an extract of the pesticide applied to the seed surface.
10 ml of this pesticide extract was added to 150 ml of the aforementioned yeast extract / malt extract medium, and liquid culture was performed at 28 ° C. for 7 days.
The influence on the growth of the A13 strain was investigated. The growth of the A13 strain was measured by placing 10 ml of the culture solution in a 13 ml Spitz tube, centrifuging at 3000 rpm for 15 minutes, and measuring the wet cell mass (packed cell volume) obtained. As a result, even when the extract of two kinds of pesticides of thiuram, thiuram and metalaxyl and the extract of three kinds of pesticides of thiuram, benomyl and captan were added to the medium for culturing A13 strain, the effect on the growth was also affected. No pesticides were found, and thiuram, metalaxyl, benomyl, and captan were resistant to these pesticides. Table 7 shows the results.

[0033]

[Table 7]

[0034]

Example 7 Controlling effect of spinach wilt of A13 strain in the presence of thiuram and metalaxyl, and in the presence of thiuram, benomyl and captan: Actinomyces streptomyces roche ess roscherii A13 and spinach wilt in Example 2 Actinomycetes A10 (Streptomyces achromogenes), which has been shown to be effective in controlling
For actinomycete A11 (Streptomyces hygroscopicus) and actinomycete A17 (Streptomyces limosus), thiuram and metalaxyl presence conditions,
The effect of controlling spinach wilt in the presence of thiuram, benomyl and captan was compared.

Actinomycetes Streptomyces roche ess roche A13, actinomycetes A10, actinomycetes A1
1. About 4 strains of actinomycetes A17, liquid culture was performed at 28 ° C. for 7 days using a yeast extract / malt extract medium. Each of the obtained culture bacterial solutions was added to a commercially available seedling cultivation soil [Nitten EE cultivation soil (made for rain repellent spinach) manufactured by Nippon Sugar Beet Co., Ltd.], and the number of bacteria per 1 g of dry soil was 6.0.
It was added so as to be × 10 ⁶ . The conditions for the presence of thiuram and metalaxyl were as follows: thiuram and metalaxyl applied to the surface of spinach seeds (“Tonic” manufactured by Watanabe Seed Co., Ltd.) were seeded in a seedling culture medium to which each actinomycete was added without washing and removing thiuram and metalaxyl. The conditions for the presence of thiuram, benomyl and captan were as follows: the thiuram, benomyl and captan applied to the surface of spinach seeds (“Active” manufactured by Sakata Seed Co., Ltd.) Seeded.

The spinach seedlings grown for 14 days were transplanted to the disease-producing soil prepared in Example 1 and cultivated using a 3 L planter under the same conditions as in Example 2. After the transplant,
On the third week, the wilt of spinach in each planter was observed, the disease rate was calculated from the number of diseased strains relative to the number of transplanted strains, and those with a small disease rate were determined to have high disease control effects. As a result, actinomycetes Streptomyces
Roche SS Roche A13 also shows that thiuram, metalaxyl, and thiuram,
Even in the presence of benomyl and captan, a higher disease control effect was observed than other actinomycetes. Table 8 shows the results. Therefore, actinomycetes Streptomyces rochei
Even when sowing seeds such as vegetables coated with these pesticides such as thiuram, metalaxyl, benomyl, and captan on field soil and nursery soil to which S.S. Roche A13 has been added, it is possible to control soil diseases without any influence. can do.

[0037]

[Table 8]

[0038]

The actinomycetes of the present invention have a sufficient control effect on soil diseases such as wilt even when seeds coated with pesticides such as thiuram, metalaxyl, benomyl and captan are used to prevent damping-off. Can be demonstrated in. In addition, the use of the culture medium to which the microorganism of the present invention is added enables the growth of seedlings that are resistant to soil diseases such as wilt.

[Brief description of the drawings]

FIG. 1 is a chart of gas chromatographic analysis of fatty acid composition of A13 strain.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A01N 47/04 101 A01N 47/04 101 47/14 47/14 A 47/38 47/38 C 63/02 63/02 G // (C12N 1/20 (C12N 1/20 C12R 1: 465) C12R 1: 465)

Claims (4)

[Claims] 1. A microorganism Streptomyces roche es ss roche A13 having a soil disease control function.
(Accession number FERM P-18169). 2. Streptomyces roche ess roche A13 is used in an amount of 10 ⁴ to 10 ⁴ g per 1 g of dry soil.
10. A soil disease-reducing soil containing 10 ¹⁰ CFU. 3. A method according to claim 1, wherein said Streptomyces roche ess roche A13 is used in an amount of 10 ⁴ to 10 g per 1 g of dry soil.
A method for raising seedlings, comprising sowing seeds of a crop in a culture medium containing 10 ¹⁰ CFU. 4. The method according to claim 3, wherein the seed is a seed coated and / or coated with at least one or more pesticides selected from thiuram, metalaxyl, benomyl, and captan. .