JP2002207034A5 - - Google Patents
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- JP2002207034A5 JP2002207034A5 JP2001002880A JP2001002880A JP2002207034A5 JP 2002207034 A5 JP2002207034 A5 JP 2002207034A5 JP 2001002880 A JP2001002880 A JP 2001002880A JP 2001002880 A JP2001002880 A JP 2001002880A JP 2002207034 A5 JP2002207034 A5 JP 2002207034A5
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- population
- cells
- platelet aggregation
- dimensional distribution
- scattered light
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Description
【請求項１】 以下の工程からなる異常細胞検出方法；
（１）試料を、血液試料中の赤血球を測定の障害とならない程度に溶解し、白血球および異常細胞を測定に好適な状態にする溶血剤と混合する工程
（２）（１）で調製した試料をフローサイトメーターで測定し、少なくとも２つの散乱光を測定する工程
（３）（２）で測定した個々の細胞の散乱光信号から異なる散乱光を２軸として、２次元分布を得る工程、
（４）２次元分布から、正常な白血球を少なくとも４つに分類計数する工程
（５）２次元分布から、リンパ球系腫瘍細胞集団、血小板凝集または大型血小板の集団、または芽球集団の分布領域を設定し、リンパ球系腫瘍細胞、血小板凝集または大型血小板、または芽球を検出する工程。
1. A method for detecting abnormal cells, comprising the following steps:
(1) The sample prepared in the step (2) (1) in which the sample is dissolved with red blood cells in the blood sample to such an extent that it does not hinder the measurement, and the white blood cells and abnormal cells are mixed with a hemolytic agent in a state suitable for measurement. Measuring with a flow cytometer and measuring at least two scattered lights (3) a step of obtaining a two-dimensional distribution using two different axes of scattered light from the scattered light signals of the individual cells measured in (2),
(4) a step of classifying and counting at least four normal leukocytes from the two-dimensional distribution; (5) a distribution area of a lymphoid tumor cell population, a platelet aggregation or large platelet population, or a blast population from the two-dimensional distribution And detecting lymphocyte tumor cells , platelet aggregation or large platelets, or blasts .
「２次元分布から、リンパ球系腫瘍細胞集団、血小板凝集または大型血小板の集団、または芽球集団の分布領域を設定し、リンパ球系腫瘍細胞、血小板凝集または大型血小板、または芽球を検出する工程」とは、例えば、Ｘ軸に側方散乱光、Ｙ軸に前方散乱光をとって、２次元分布を描くと、図１に示すように、リンパ球系腫瘍細胞は集団を形成する。この集団を適当な解析ソフトで解析することにより、リンパ球系腫瘍細胞集団の数を算出する。血小板凝集、大型血小板、芽球も同様にして、各集団の数を算出する。各集団の数にはそれぞれ閾値を設定しておき、その設定値を超えたらメッセージを表示する。
"From the two-dimensional distribution, set the distribution area of the lymphoid tumor cell population, platelet aggregation or large platelet population, or blast population to detect lymphoid tumor cells , platelet aggregation or large platelets, or blasts In the "step", for example, when a two-dimensional distribution is drawn by using side scattered light on the X axis and forward scattered light on the Y axis, the lymphoid tumor cells form a population as shown in FIG. The number of lymphocyte tumor cell populations is calculated by analyzing this population with appropriate analysis software. The number of each group is calculated in the same manner for platelet aggregation, large platelets, and blasts. A threshold value is set for the number of each group, and a message is displayed when the threshold value is exceeded.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001002880A JP4484375B2 (en) | 2001-01-10 | 2001-01-10 | Abnormal cell detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001002880A JP4484375B2 (en) | 2001-01-10 | 2001-01-10 | Abnormal cell detection method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002207034A JP2002207034A (en) | 2002-07-26 |
| JP2002207034A5 true JP2002207034A5 (en) | 2008-02-14 |
| JP4484375B2 JP4484375B2 (en) | 2010-06-16 |
Family
ID=18871313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001002880A Expired - Lifetime JP4484375B2 (en) | 2001-01-10 | 2001-01-10 | Abnormal cell detection method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4484375B2 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6743634B2 (en) | 2002-08-23 | 2004-06-01 | Coulter International Corp. | Method and apparatus for differentiating blood cells using back-scatter |
| FR2935802B1 (en) * | 2008-09-05 | 2012-12-28 | Horiba Abx Sas | METHOD AND DEVICE FOR CLASSIFYING, VISUALIZING AND EXPLORING BIOLOGICAL DATA |
| WO2010071114A1 (en) * | 2008-12-18 | 2010-06-24 | シスメックス株式会社 | Method for detecting cancer cells in blood sample |
| JP5583629B2 (en) * | 2011-04-28 | 2014-09-03 | シスメックス株式会社 | White blood cell classification and counting method, white blood cell classification reagent kit, and white blood cell classification reagent |
| EP2520926B1 (en) * | 2011-05-05 | 2022-06-15 | Sysmex Corporation | Blood analyzer, blood analysis method, and computer program product |
| JP2012088336A (en) * | 2012-01-23 | 2012-05-10 | Sysmex Corp | Method and device for discriminating myeloblast from platelet aggregation |
| CN107576634B (en) * | 2013-07-16 | 2021-07-23 | 成都深迈瑞医疗电子技术研究院有限公司 | Blood cell analyzer and cell identification method and system thereof |
| JP6710512B2 (en) * | 2015-09-14 | 2020-06-17 | シスメックス株式会社 | Blood analysis device, blood analysis method, and computer program |
| CN110383037B (en) | 2017-06-20 | 2022-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | Platelet aggregation identification method and device and cell analyzer |
-
2001
- 2001-01-10 JP JP2001002880A patent/JP4484375B2/en not_active Expired - Lifetime
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