JP2002183467A - Consigment sale method of gene containing dna micro chip - Google Patents

Abstract

PROBLEM TO BE SOLVED: To achieve a genetic research and a genetic diagnosis without an expensive device or equipment by designing a DNA micro chip that a customer requires through the Internet, and by selecting a partial charge of work through gene detection profiling analysis to order from a marker. SOLUTION: Using a genetic information database 13 wherein the genes are classified to plural items containing kinds, tissues, and functions to be searched, the customer 15 selects a desired gene and designs a DNA chip A from the database 13. Then, the partial charge of the work is designated with respect to the plural work containing the production of the DNA chip, hybridization, gene detection profiling analysis and physiological analysis gene order to be ordered. The marker 12 conducts the prescribed work in accordance with the ordered partial charge. The obtained information is encoded and transferred to the customer on the Web.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to designing a DNA microchip required by a wide range of customers, including scientific researchers and physicians, as well as designing a DNA microchip, analyzing gene expression, and analyzing genes for physiological analysis. It is possible to specify the work sharing leading to the preparation and place an order with the manufacturer, and the manufacturer manufactures the DNA microchip and the gene for physiological analysis and receives the data obtained by performing the work according to the work sharing to the customer. The present invention relates to a method for consigned sales of DNA microchips that are conveyed to a company and that can be safely stocked and used.

[0002]

2. Description of the Related Art There has been a remarkable progress in research and development relating to genes or in medical treatment including diagnosis using genes. For example, gene recombination technology has enabled the production of foods useful for humankind and the development of pharmaceuticals.
In addition, by performing genetic diagnosis, it becomes possible to predict the onset of a disease including many genetic factors, and it is possible to identify the cause. Along with this, it has become possible to radically cure diseases previously unknown, to develop drugs, and to postpone the onset by implementing preventive living improvements.

[0003] When conducting genetic research or genetic diagnosis, a plurality of known genes corresponding to the type, organization, and function of the gene to be studied or diagnosed, and a gene containing an unknown base sequence are used. . In this case, a plurality of appropriate known genes and unknown genes are selected according to the respective types, tissues, and functions.
In addition, it is often handled as a DNA microarray in which the selected optimal ones are arranged.

[0004] For example, when performing a gene diagnosis, it is necessary to perform an operation of performing hybridization on a sequenced gene and performing gene expression profiling analysis. In addition, in the process of performing the above-mentioned operations, operations such as preparation of a large number (hundreds to tens of thousands) of genes for preparing a DNA microarray and operation of increasing the production of these genes in large quantities are added.

[0005] When conducting genetic research or genetic diagnosis which requires the above-mentioned operations, expensive machinery and equipment are required, and some of the equipment is extremely limited due to problems in equipment and work space. It is the fact that it is being done at the institution. In many of these institutions, each has the ability to perform each of the above-mentioned operations, and gene trading involves transferring a desired gene alone or, for example, by placing DNA on a glass substrate.
It is performed as a DNA microchip on which a microarray is formed.

[0006] The DNA microchip is used to select genes required for the purpose of gene research or genetic diagnosis, and to transfer the selected genes onto a substrate using a mechanical device called an arrayer. DNA on top
It is configured by forming a microarray. This DNA
In the microchip, a plurality of known genes and unknown genes are arranged and fixed in accordance with predesigned conditions, and can be distributed by ordinary distribution means in this state.

[0007] A procedure when a customer intends to conduct genetic research or genetic diagnosis will be described. The customer obtains information on the genes necessary for the intended research from catalogs and magazines published by the manufacturer or from the catalogs and magazines published by the manufacturer, confirms the genes possessed based on the acquired information, and identifies the genes not possessed. An order is placed with a manufacturer, and a DNA microchip is manufactured by attaching the gene on the substrate. In addition, the manufacturer that received the order for the gene manufactures a DNA microchip in which the ordered gene is attached to a substrate.
Delivery to customers in microchip form. The customer who has obtained the DNA microchip collects the required sample, performs hybridization with the sample, collects scanning data with a scanner, analyzes the collected data, and stores the obtained data in a data bank. And ordering of genes for sanitary analysis.

[0008] When performing the above series of operations, DN
When manufacturing an A microchip, an apparatus called an arrayer is required. When performing hybridization, a hybridization technique specific to the work is required. When performing scanning, a scanner is required. .

[0009]

As described above, in the case of conducting scientific research or medical diagnosis by directly handling genes, it is necessary to install various kinds of expensive machinery and equipment. Therefore, there is a problem that a relatively small tissue, for example, a personal researcher or medical practitioner cannot perform genetic research or genetic diagnosis.

[0010] Even among researchers and physicians belonging to large institutions, only a few DNA microchips are currently sold in Japan, and there are very few outsourced DNA microchip manufacturers. There is also a problem that it is difficult to obtain a suitable DNA microchip. This is because advanced functional analysis using a DNA microchip has just begun,
Equipment such as an arrayer for producing an NA microarray is expensive, and furthermore, gene expression profiling analysis technology using a DNA microarray system is in the process of development and the degree of completion is immature.

According to the current flow of research, regarding genes identified by individual researchers by gene expression analysis, if the genes are not retained or are difficult to obtain, the genes are expressed. MRN from a certain organization
Generally, A is extracted, the extracted gene is cloned, and further subcloned for physiological analysis. However, the work of gene cloning and subcloning for use in various physiological studies involves the process of mass production of genes by the PCR (Polymerase Chain Reactin) method and the process of determining the nucleotide sequence of cloned genes. Inevitably included multiple times, it takes a lot of manpower and time, and is a factor that raises costs.

[0012] Furthermore, in order to rapidly and at a low cost perform gene expression analysis that is expected to be an astronomical information amount, it is not necessary to impose a DNA microchip on a researcher or a doctor according to the manufacturer's theory. , With researchers and doctors
Experts who have established NA microchip technology can quickly and smoothly perform various tasks and information exchange on DNA microchips and genes required by individual researchers and doctors according to the intentions of the researchers and doctors. It is hoped that it can be done.

In particular, when the genome project ends,
An enormous amount of genetic information is made available worldwide. This genetic information is not limited to research in biology and basic medicine, and is expected to make a great contribution in a wide range of fields including clinical medicine. Therefore, it is urgently necessary to quickly and safely communicate the released genetic information and to prepare an environment for stocking the information.

An object of the present invention is to first design a DNA microchip required by a customer via the Internet, and to select an allotment of work to be performed up to gene expression profiling analysis and place an order with a manufacturer. Thus, it is possible to provide a consignment sale method of a gene including a DNA microchip, which enables the realization of genetic research and gene diagnosis without expensive equipment and facilities. Second, a gene for DNA microarray and a gene for physiological analysis are provided. By combining both, the time required to shift from gene expression analysis to physiological function analysis of the gene product can be significantly shortened, thereby improving research speed and reducing costs. Gene expression analysis that provides a huge amount of information by providing a database or providing a database creation service Distribution and customers to easily use management is to provide a consignment method of gene comprising a DNA microchip such permits.

[0015]

According to the present invention, there is provided a method for consigning and selling a gene including a DNA microchip according to the present invention. Using the obtained gene information, the customer selects a desired gene from the gene information to design a unique DNA microchip, and also transcribes the gene onto a substrate, hybridizes, analyzes gene expression profiling, and analyzes the physiology. For a plurality of operations including an order for a gene, an order is assigned by designating the work sharing between the customer and the manufacturer, and the manufacturer performs predetermined work according to the work sharing selected by the customer, and performs the predetermined work. The obtained information is transmitted to a customer on a web.

[0016] In the above-mentioned method of contract sale of a gene containing a DNA microchip (hereinafter, simply referred to as “consignment sale method”), the gene is classified into a plurality of items including types, tissues, and functions and can be searched. Information databases and websites of gene research institutions and universities (hereinafter collectively referred to as "gene information databases" or simply "databases"
By using this method, customers including researchers conducting genetic research and physicians performing genetic diagnosis and gene therapy can use the genetic information recorded in the genetic information database to conduct research, diagnosis and treatment (hereinafter referred to as “research etc.”). )) To design a unique DNA microchip.

[0017] Further, the customer is required to carry out gene transfer work onto a substrate necessary for producing a designed DNA microchip and to conduct a target research in accordance with various conditions such as the contents of the target research or the like and the capacity of the equipment possessed. Whether the customer or the manufacturer performs multiple tasks, including the tasks of hybridization, gene expression profiling analysis, etc., required to collect vital data, and the task of ordering genes for physiological analysis. You can specify the work sharing and place an order with the manufacturer.

For this reason, the manufacturer performs predetermined work in accordance with the work assignment received, and obtains the DNA generated in this process.
By sending products containing microchips to customers, or by transmitting the acquired information to customers on the web, customers can obtain products or information in line with the contents of the intended research, etc., and according to the equipment capabilities they possess. Can be obtained.

In the above-mentioned consignment sales method, the genes held by the manufacturer in the data bank hold both genes for DNA microchip preparation and genes for physiological analysis including full-length cDNA, It is preferable to supply a gene for physiological analysis immediately after gene expression profiling analysis.

When the customer or the maker performs hybridization of the DNA microchip and the maker reads the data of the DNA microchip, and then performs the gene expression profiling analysis by the customer, the maker reads the DNA microchip. It is preferable to transfer the read data of the DNA microchip to the customer on the web and provide analysis software, and the customer analyzes the read data of the DNA microchip transferred on the web based on the provided analysis software.

In particular, DNA including DNA microarray
When transferring microchip data on the web,
Preferably, the data is encrypted. By encrypting data in this manner, confidentiality can be maintained, and leakage of information on genes can be prevented.

Further, when a customer provides a sample containing a nucleic acid to a manufacturer and performs gene expression profiling analysis, the manufacturer performs gene expression profiling analysis using the sample provided by the customer, and then obtains the obtained data. Preferably, it is forwarded to the customer on the web.

When a customer provides a sample containing nucleic acid to a manufacturer and performs gene expression profiling analysis, the manufacturer prepares a database of the results of the gene expression profiling analysis and provides the result to the customer on the web, or the customer himself or herself. When the gene expression profiling analysis is performed, it is preferable that the maker provides the customer with software for creating a database of the gene expression profiling analysis results, or an environment on which the database can be created.

[0024]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The above-mentioned contract sales method will be described below with reference to the drawings. FIG. 1 is a diagram for explaining the relationship when the commissioned sales method according to the present invention is performed. FIG. 2 is a flowchart for explaining a first embodiment for executing the consignment sales method. FIG. 3 is a flowchart illustrating the second embodiment. FIG. 4 is a flowchart illustrating the third embodiment. FIG. 5 is a flowchart illustrating a procedure for tender purchase of a gene. FIG. 6 is a diagram illustrating a DNA microchip and a DNA microarray.

In the present invention, the object of the transaction is D
Gene containing NA microchip, not necessarily DN
The A microchip itself is not limited as a transaction target. That is, it is possible to trade the gene required by the customer alone or in the form of a DNA microarray in which a plurality of genes are arranged in parallel. However, since the description is complicated, a plurality of genes are arranged in parallel on
A case where a DNA microchip on which an NA microarray is formed is traded will be described.

Prior to the description of the consignment selling method according to the present invention, the DNA microchip A and the DNA microarray B will be described with reference to FIG.

The DNA microchip A has a substrate 1 made of a transparent plastic plate or a glass plate, and is formed by forming points 2 on which a gene for a reagent is attached. The number of points 2 attached to the base 1 is not particularly limited, and one or many points 2 can be attached under a certain condition.

The DNA microarray B is composed of a plurality of points 2 where reagent genes are attached in parallel. In this case, the material or shape of the base to which the point 2 is attached is not particularly limited, and the base 1 or a sheet-like material such as paper or nonwoven fabric, or a sheet-like material made of an inorganic material such as metal is used. It is possible to

In this embodiment, the base 1 made of a glass plate is used.
Then, a 10 mm square area is set and a number of points 2 are set at 0.2 mm pitch.
A DNA microarray (hereinafter "DN
A array B) is formed, and a DNA microchip (hereinafter, “DNA chip”) A is formed.

Next, several embodiments of a method for consigned sale of a gene containing a DNA chip A will be described with reference to FIGS.

In FIG. 1, 11 indicates the Internet, 12 indicates a maker, and 13 indicates a database.
Reference numerals 14a and 14b denote research institutions such as research laboratories and hospitals that conduct research and storage on genes, and reference numerals 15a to 15c denote customers comprising laboratories and medical institutions that require the gene itself or genetic information. I have. Further, a solid line indicates a communication network for information and data (hereinafter referred to as a “communication network”) via the Internet 11 formed between the maker 12, the research institutes 14a and 14b, and the customers 15a to 15c. 1 shows a gene distribution network (hereinafter, referred to as a “distribution network”) formed between the laboratory 12 and the research institutes 14a and 14b, and the two-dot chain line includes the DNA chip A formed between the manufacturer 12 and the customers 15a to 15c. This shows the sales network (hereinafter referred to as “sales network”) that includes the distribution of genes and the flow of decisions.

The database 13 is composed of a plurality of items including gene types, tissues, and functions, and is configured so that required data can be collected using any of the related items as keywords. It is not limited which of the maker 12 and the research institutes 14a and 14b manages the database 13, but in each of the embodiments described later, the maker 12 manages the database 13.

Next, prior to describing a specific embodiment of the consignment sales method of the present invention, the procedure for producing a DNA chip by a customer and the fact that neither the customer nor the manufacturer possesses a necessary gene. For this reason, a procedure for purchasing a necessary gene in a public manner will be briefly described with reference to FIG.

The types of genes are enormous, and information including the types, tissues, and functions of these genes is stored in a database.
Even if it has been published on 13, the actual gene
There is no guarantee that 〜15c or maker 12 has it all. Therefore, it is necessary for the customers 15a to 15c to openly purchase genes not owned by the customer and the manufacturer 12 in the process of designing and manufacturing the DNA chip.

In FIG. 5, a customer 15 (any of 15a to 15c) is assumed to have a relatively substantial research organization, and may have a device for manufacturing a DNA chip. It is assumed that the customer 15 does not have a gene that is optimal for the purpose of research, but the DNA chip A can be manufactured independently if the gene is available.

When the customer 15 needs a gene for conducting a physiological study (step S11), first, gene information necessary for a target physiological study is collected (step S1).
2). At this time, for the genetic information not held by the customer 15, the data is collected by accessing the database 13 using the communication network.

After collecting all the gene information necessary for the intended physiological study, the DNA chip A is designed including the sequence of these genes (step S1).
3). In the design of the DNA chip A, genes optimal for the researcher to proceed with the intended research are arranged most rationally.

When a designed DNA chip A is manufactured, it is necessary to have a gene to be attached to the DNA chip A. For this reason, the gene possessed is examined in step S14, and if possessed, the process proceeds to step S15 to confirm the presence or absence of a device for producing a DNA chip, and if not possessed, a communication network is established. Use and place an order with Maker 12.

When all the genes are retained and the process proceeds to the production of the DNA chip A, it is determined in step S15 whether or not the apparatus for producing the DNA chip is possessed. Proceeding to S16, a DNA chip A is manufactured, and if it is not owned, the manufacturer 12 is requested.

The maker 12 receiving the gene order and the production request of the DNA chip A from the customer 15 proceeds to step S17 to investigate the genes possessed by the maker 12,
If all the ordered genes are possessed, step S
Proceed to 18 to manufacture a DNA chip A.

If the gene does not have the ordered gene, the manufacturer 12 performs public collection using the communication network (step S19). At this time, information on the gene to be collected and the transfer conditions are disclosed (step S20).

The gene holder (for example, a research institution) who has seen the gene information and the transfer conditions disclosed in steps S19 and S20 is the manufacturer 12 or the customer 15 via the manufacturer 12
Whether the transaction conditions are free of charge (step S21),
If it is paid (step S22), if it is paid, it is one-time payment, installment payment or royalty, or customer
Negotiations on transfer conditions, including whether or not to conduct joint research with Step 15 and the gene holding organization (step S23), etc., and when mutual agreement is reached, data on the target gene is transmitted from the research institution via the communication network. Conveyed to customer 15. In addition, the gene is delivered from the research institution to the manufacturer 12 via a distribution network,
Alternatively, when the customer 15 has a DNA chip manufacturing device, the product is directly delivered to the customer.

Genes obtained by the maker 12 through steps S19 to S23 are increased in mass by applying, for example, the PCR method, and the rest attached to the DNA chip A is stored in the maker 12.

The decision on the gene obtained by the customer 15 is as follows:
It is performed via a communication network or a sales network according to transfer conditions.

Through the above series of procedures, the customer 15 can design the DNA chip A including the target gene and manufacture the DNA chip A based on the design.

When data is transferred on the web via the Internet constituting a communication network, all data is encrypted. In particular, when data of the DNA chip A including the DNA array B is transferred, the transferred data is encrypted when confidentiality is required. Encrypting when the confidentiality of the data relating to DNA is required is handled in the same manner in each of the embodiments described below as well as this embodiment.

In the figure, the part described as "web" is a work or operation performed through a communication network including the Internet. And customer as mentioned above
15. If the confidentiality of data transferred between manufacturers 12 is required, everything is encrypted.

FIG. 2 is a flowchart of the first embodiment. In this embodiment, the customer 15 is a DNA customer.
All operations from chip production to scanning are outsourced to the manufacturer 12. In the figure, the physiology research at step S31 and the DN at step S33 are performed.
The process up to the A-chip design is the same as the above-described example of the tender offer, and the description is omitted.

In step S34, the work required for the DNA chip A designed for the purpose of physiological research in the steps up to step S33 is examined, and the work performed independently by the customer 15 and entrusted to the manufacturer 12 are performed. Classify work. That is,
The task of collecting the genes that make up the designed DNA chip A, the task of manufacturing the DNA chip A, the task of collecting various experiments and samples necessary for the intended physiological research, and the work of hybridization, scanning, and data analysis From among a plurality of works including the above, work classified by the customer 15 and work to be entrusted to the manufacturer 12 are classified.

As a result, when it is determined that the operation up to the scanning is entrusted to the manufacturer 12 (step S35).
-Step S37), RNA (messenger RNA, mRNA) of the sample is collected in step S41, and delivered to the manufacturer 12 via the sales network.

In the maker 12, the DNA designed by the customer 15
A series of operations from chip A production to scanning are commissioned (Step S38, Step S40, Step S4)
3), work progress. At this time, if the maker 12 does not have the gene necessary for producing the received DNA chip A, the same operation (open offer) as in steps S19 to S23 in the example of FIG. Collect genes.

Accordingly, the maker 12 manufactures the received DNA chip A according to the same procedure as in the example of FIG. 5 described above (step S39). Thereafter, hybridization is performed in step S43 using the RNA of the sample delivered from the customer 15, and scanning is performed in step S44. Then, by transferring the scanning data obtained by the scanning to the customer 15 (step S46),
A series of commissioned work by the manufacturer 12 is completed.

The hybridization performed in step S43 is performed on the DNA array B constituting the DNA chip A.
In this method, RNA of a sample is caused to act on the DNA to hybridize it with complementary DNA, and the RNA of the sample is fluorescently labeled with a fluorescent material such as a fluorescent dye to perform a binding treatment to the DNA array B.

The scanning performed in step S44 is performed for the DNA array B constituting the DNA chip A.
The amount of fluorescence of each DNA constituting the DNA array B is measured by scanning the excitation light irradiated from the scanner, and each DNA
Position information and fluorescence amount data (scanning data)
Is stored in the storage device. After measuring the amounts of fluorescence of all the DNAs, the process proceeds to step S43. At this time, DN
The larger the amount of binding of the sample RNA to the DNA constituting the A array B, the greater the amount of fluorescence.

In step S46, the data of each DNA stored in the storage device is transferred to the customer 15.

The customer 15 to whom the scanning data has been transferred
Examines the presence or absence of the scanning data analysis software in step S47, and if not, requests the manufacturer 12 to provide the scanning data analysis software. The maker 12 receiving the request transfers necessary scanning data analysis software to the customer 15 (step S48).

After that, the customer 15 analyzes the scanning data using the provided scanning data analysis software (steps S49 and S50). This work analyzes the data of each DNA and the data of the amount of fluorescence stored in the storage device, and identifies the DNA complementary to the RNA of the sample from the amount of binding to the DNA constituting the DNA array B. Then, by knowing the properties of the specified DNA from the sequence position in the DNA array, the properties or characteristics of the RNA of the specimen are investigated.

After the scanning data analysis is completed,
Proceeding to step S51, the scanning data is converted into a data bank. Thereafter, the process proceeds to step S52 to order the sanitary analysis gene, thereby completing a series of operations unique to the customer 15.

The gene held in the data bank is DN
It is composed of a gene for producing an A chip and a gene for physiological analysis including full-length cDNA, and is managed so that it can be promptly provided to customers as needed.

Next, FIG. 3 is a flowchart of the second embodiment. In this embodiment, the customer 15 performs the hybridization work on the customer side, and entrusts the production and the scanning work of the DNA chip A to the manufacturer 12. It is assumed. In the figure, steps having the same contents as those in the above-described first embodiment are denoted by the same reference numerals, and description thereof is omitted.

As shown in the figure, the steps from step S31 to step S34 until the customer designs the DNA chip A, examines the work, and requests the maker 12 to manufacture the DNA chip A, and steps S38 and S39. Manufacturers
The steps for fabricating the DNA chip A accepted by 12 are the same as those in the first embodiment.

After producing the DNA chip A, the manufacturer 12 delivers the produced DNA chip A to the customer 15 via the distribution network, confirms in step S60 that there is no request for hybridization, and proceeds to step S65. And wait.

The customer 15 having received the DNA chip A collects the RNA of the sample in step S61, and performs a hybridization operation in which the RNA of the sample is caused to act on the DNA array B of the manufactured DNA chip A and hybridized (step S61). S62, 63). Thereafter, the process proceeds to step S64 to check for the presence or absence of a scanner, to confirm that the scanner is not provided, and to determine the DNA after hybridization.
The chip A is delivered to the maker 12.

The manufacturer 12 confirms the scanning request from the customer 15 in step S65, scans the delivered DNA chip A after hybridization, and creates scanning data (steps S66 and S67). Then, the created scanning data is transferred to the customer 15 on the web.

Customer 15 receiving scanning data
Analyzes the scanning data using the provided scanning data analysis software (step S
68) Investigate the properties or characteristics of the RNA of the sample.

After the scanning data analysis is completed,
Proceeding to step S51, the scanning data is converted into a data bank. Thereafter, the process proceeds to step S52 to order a physiological analysis gene.

FIG. 4 is a flowchart of the third embodiment, in which the customer 15 in this embodiment requests the maker 12 for all operations including the production of the DNA chip A. In the figure, steps having the same contents as those in the above-described first embodiment are denoted by the same reference numerals, and description thereof is omitted.

In the figure, step S31 performed by the customer 15
Contents of the step of examining the division of work between the customer 15 and the maker 12 for the designed DNA chip in step S34 from the physiological study of step S34, and the step D
The contents of the steps from the production of the NA chip A to the conversion of the scanning data into a data bank in step S51 are the same as those in the first embodiment.

The customer 15 outsources the production of the DNA chip A to the maker 12 in step S70, and performs all the operations of the hybridization work (step S71), the scanning work (step S72) and the scanning data analysis work. Outsource to In this case, the customer 15 collects the sample in step S73, and obtains the blood, cells, and R of the collected sample.
One of the NAs is delivered to the maker 12 via the distribution network.

In the manufacturer 12, the DNA chip A
After collecting the genes not possessed according to the design contents of the above, a DNA chip A is manufactured, and the RNA is determined according to the type of the sample (blood, cell, RNA) sent from the customer 15.
Is collected, a hybridization operation is performed in which the RNA collected from the sample is allowed to act on the produced DNA chip A to perform hybridization, and the DNA array B of the hybridized DNA chip A is scanned, followed by data analysis. (Step S38 to Step S
50). Thereafter, the process proceeds to step S51, where the analysis data is
It is directly used for clinical use or drug discovery, and is made into a data bank. These series of operations are the same as the operations of the first embodiment described above.

As described above, when a gene is contracted for sale,
There are several stages depending on the technology and equipment possessed by the customer 15, but it is possible to deal with each stage in any way.

[0072]

As described in detail above, the consignment sales method according to the present invention uses a gene information database in which genes can be classified and searched for in a plurality of items including types, tissues, and functions. Customers, including researchers who conduct genetic research and physicians who perform genetic diagnosis and gene therapy, can conduct research, diagnosis, and treatment (hereinafter referred to as “research etc.”) based on the genetic information recorded in the genetic information database.
) To design a unique DNA microchip.

Further, the customer is required to carry out gene transfer work onto a base necessary for producing a designed DNA microchip or to conduct a target research in accordance with various conditions such as the contents of the target research and the capacity of the equipment owned. Whether the customer or the manufacturer performs multiple tasks, including the tasks of hybridization, gene expression profiling analysis, etc., required to collect vital data, and the task of ordering genes for physiological analysis. You can specify the work sharing and place an order with the manufacturer.

For this reason, the manufacturer performs predetermined work according to the work assignment received and receives DNA generated in this process.
By sending products containing microchips to customers, or by transmitting the acquired information to customers on the web, customers can obtain products or information in line with the contents of the intended research, etc., and according to the equipment capabilities they possess. Can be obtained in a short time.

When the customer or the maker performs hybridization of the DNA microchip and the maker reads the data of the DNA microchip, and then performs the gene expression profiling analysis by the customer, the maker reads the DNA microchip. And the analysis data is provided to the customer on the web, and the customer can analyze the read data of the DNA microchip transferred on the web based on the provided analysis software.

In particular, DNA containing DNA microarray
When transferring microchip data on the web,
Preferably, the data is encrypted. By encrypting data in this manner, confidentiality can be maintained, and leakage of information on genes can be prevented.

When a customer provides a sample containing a nucleic acid to a manufacturer and performs gene expression profiling analysis, the manufacturer performs gene expression profiling analysis using the sample provided by the customer, and then obtains the obtained data. In the case where the data can be transferred to the customer on the web and the gene expression profiling analysis is performed, the manufacturer makes the database of the result of the gene expression profiling analysis and provides the result to the customer, or the customer performs the gene expression profiling analysis. Manufacturers can provide their customers with software for creating database of gene expression profiling analysis results, enabling them to build a common database between customers and manufacturers, and easily manage cumbersome data. Is completed,
Work efficiency can be improved. In addition, manufacturers can hold a large amount of data, and provide high quality and quantity services to customers.

[Brief description of the drawings]

FIG. 1 is a diagram for explaining the relationship when implementing a consignment sales method according to the present invention.

FIG. 2 is a flowchart illustrating a first embodiment for executing the consignment sales method.

FIG. 3 is a flowchart illustrating a second embodiment.

FIG. 4 is a flowchart illustrating a third embodiment.

FIG. 5 is a flowchart illustrating a procedure for tenderly purchasing a gene.

FIG. 6 is a diagram illustrating a DNA microchip and a DNA microarray.

[Explanation of symbols]

 A DNA chip B DNA array 1 Base 2 points 11 Internet 12 Manufacturer 13 Database 14a, 14b Research institution 15, 15a-15c Customer

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Claims (6)

[Claims] 1. Using a gene information which can be searched by classifying into a plurality of items including a kind, a tissue, and a function of a gene, a customer selects a desired gene from the gene information and selects a unique DNA microchip. Designing and ordering multiple tasks, including transcription of genes on the substrate, hybridization, gene expression profiling analysis, and ordering of genes for physiological analysis, by specifying the work sharing between the customer and manufacturer, A method for contract sale of a gene including a DNA microchip, wherein a manufacturer performs a predetermined operation in accordance with a task allocation selected by a customer, and transmits information obtained by performing the predetermined operation to the customer on a web. 2. Genes stored in a data bank by a manufacturer include both genes for preparing a DNA microchip and genes for physiological analysis including full-length cDNA, and after a large amount of gene expression profiling analysis using a microarray. The method of claim 1, wherein the gene for physiological analysis is promptly supplied. 3. The method according to claim 1, wherein a customer or a maker carries out hybridization of the DNA microchip, and
When the customer performs gene expression profiling analysis after the maker has read the data of the microchip, the maker transfers the read data of the read DNA microchip to the customer on the web and provides analysis software, and 3. The method of claim 1, wherein the customer analyzes the read data of the DNA microchip transferred on the web based on the provided analysis software. 4. A gene comprising a DNA microchip according to claim 1, wherein the data is encrypted when transferring the data of the DNA microchip including the DNA microarray on the web. Contract sales method. 5. When a customer provides a sample containing a nucleic acid to a manufacturer and performs gene expression profiling analysis, the manufacturer performs gene expression profiling analysis using the sample provided by the customer, and then obtains the obtained data. 5. The method according to claim 1, wherein the gene is transferred to a customer on a web. 6. When a customer provides a sample containing a nucleic acid to a manufacturer and performs gene expression profiling analysis, the manufacturer prepares a database of the gene expression profiling analysis results and provides the database to the customer, or the customer himself / herself. When performing gene expression profiling analysis,
The method according to any one of claims 1 to 5, wherein the maker provides a customer with software for creating a database of the results of gene expression profiling analysis on a web.