JP2002162401A - Autoanalyzer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an autoanalyzer which realizes trace sample dispensation, while maintaining high processing capacity, as it is and reducing the consumption of dummy sample. SOLUTION: This autoanalyzer is constructed, so that sample dispensing methods are each used in its proper way according to the required amount of liquid sample for analysis and also the order of analysis is permuted according to the dispensing methods. The amount of reagent consumed can be reduced, reduction in running cost can be realized, and the time required for measurement can be shortened.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an automatic analyzer for automatically analyzing components such as blood and urine.

[0002]

2. Description of the Related Art An automatic analyzer for analyzing components such as blood and urine chemically reacts a liquid sample dispensed into a reaction container with a reagent, and irradiates the reaction solution with light from a halogen lamp or the like to measure the absorbance. It measures and analyzes the components of the liquid sample. At present, there is a demand for a reduction in running cost by a reduction in reagent consumption from a customer, and thus, a reduction in the amount of a reaction solution for realizing a reduction in reagent consumption per item has become an issue. In order to reduce the amount of the reaction solution while maintaining the current ratio of the reagent to the sample, it is necessary to reduce the amount of the sample dispensed.

[0003] In addition, it is applicable to a small amount of sample such as a pediatric sample,
In order to reduce the burden on the patient in sampling and to reduce biological pollutants, it is important to reduce the amount of each sample of the sample and to reduce the total consumption in the automatic analyzer. That is, in order to reduce the consumption of the sample, it is necessary to reduce the consumption of the sample per item and to reduce the useless sample in the sample dispensing.

[0004] In order to reduce the consumption of a sample per item, a technique for dispensing a small amount of sample is important. A conventional sample dispensing method will be described with reference to FIG. 4. In addition to the sample for measurement 405, a sample that has been sucked extra from the sample cup to eliminate the effect of thinning of the sample (hereinafter, referred to as a dummy sample 404)
Was sucked, and only the measurement sample 405 was discharged on the reaction cell. In this dispensing method, the sample s _out1 407 attached to the outer wall of the probe after aspirating the sample is brought into the reaction cell, and the sample is returned after ejection s _out2 408. Is difficult to achieve with high accuracy.

[0005] As a method for realizing a small amount dispensing of less than 2 µl, Japanese Patent Application Laid-Open No. 62-228954 discloses a method in which a probe is attached to a probe by sucking the sample and washing the outer wall of the probe with a washing liquid to remove the sample attached to the outer wall. A method for reducing a decrease in accuracy due to the introduction of a sample has been proposed. As shown in FIG. 5, the method of sucking only the measurement sample 404 and extruding the sample into the reaction cell with the system water 403 in the probe has the effect of washing the outer wall of the probe at the time of discharge, so that the probe outer wall adheres. It is also possible to reduce the carry-out of the sample, which is effective for reducing the dispensing of a small amount of sample and the mixing (contamination) with a different sample due to the carry-out of the sample (called carry-over). However, in this sample dispensing method, there is a problem in that the sequence of one sample dispensing becomes more complicated than in the dispensing method using the dummy sample 404 described above, and it takes more time and the processing capacity is reduced.

[0006] It is effective to reduce the amount of sample wasted by dispensing as well as the amount of sample dispensed for each item, which is effective in reducing the amount of sample consumption, and is a dummy sample which is a non-measurement sample for preventing thinning. The amount needs to be minimized. If the sample dispensing method for all the measurement items is the same sample and the dummy sample is used, the dummy sample is sucked in addition to the sample for the first measurement only, and only the sample for measurement is discharged to the reaction cell. And
After that, since only the sample for measurement is sucked and discharged, the consumption of the dummy sample is only required for the first one time. However, if measurement items that use a dispensing method that extrudes and discharges a sample with system water are mixed between measurement items that require a dummy sample with one dispenser, the probe needs to be cleaned. Become. Therefore, it is necessary to aspirate the dummy sample every time at the beginning of the item using the dummy sample, and the waste sample is discarded before the measurement item using the dispensing method in which the sucked dummy sample is pushed out with the system water, so that there is much waste.

[0007] Regarding the registration of the measurement order, refer to Japanese Patent Laid-Open No. 5-2 / 5-2.
Japanese Patent Publication No. 024 proposes a method of performing measurement from an item having a long analysis processing time, whereby all measurement results for each sample can be output in the shortest time.

[0008]

In the prior art, regarding the determination of the order of measurement of the sample analysis items, since the sample dispensing method is not taken into consideration, other items are separated between items by sample dispensing using a dummy sample. When the items using the injection method are mixed, there is a problem that the re-suction of the dummy sample is required, and the wasteful consumption of the sample increases.

The present invention solves the above-mentioned problems, has a high throughput, realizes the dispensing of a small amount of sample, for example, less than 2 μl, minimizes the consumption of the sample, and performs an efficient analysis. It is an object of the present invention to provide an automatic analyzer capable of performing the above.

[0010]

In order to achieve the above object, according to the present invention, there is provided an automatic analyzer having a dispensing probe for dispensing a liquid sample into a reaction vessel, and an analyzer for analyzing a reaction solution of a reagent and a sample. In the apparatus, the dispensing method of the liquid sample is performed according to an analysis item of the sample, wherein the dispensing probe aspirates a sample more than the amount required for analysis and discharges only the amount required for analysis. Or, only the amount of sample required for analysis is aspirated by the dispensing probe, and the aspirated sample is
The sample dispensing method of extruding and discharging with liquid other than the sample,
It was configured to use either one.

Further, in an automatic analyzer having a dispensing probe for dispensing a liquid sample into a reaction container and an analyzer for analyzing a reaction solution of a reagent and a sample, the liquid is supplied in accordance with the amount required for analyzing the sample. The sample dispensing method may be such that the sample dispensing probe aspirates a sample more than the amount required for analysis and discharges only the amount required for analysis, or only the amount required for analysis by the dispensing probe. A sample was aspirated, and the aspirated sample was extruded with a liquid other than the sample and ejected.

Further, in an automatic analyzer having a dispensing probe for dispensing a liquid sample into a reaction container and an analyzer for analyzing a reaction solution of a reagent and a sample, the dispensing method of the liquid sample is as follows.
When the amount of the sample to be dispensed is equal to or more than a predetermined amount, a sample dispensing method is used in which the sample dispensing probe aspirates a sample more than the amount required for analysis and discharges only the amount required for analysis. If the amount of the sample to be dispensed is less than a predetermined amount, the dispensing probe aspirates only the amount of the sample required for analysis, and extrudes the sucked sample with a liquid other than the sample and discharges the sample. It was configured to use differently depending on the analysis item of the sample to use dispensing.

A dispensing probe for dispensing a liquid sample into a reaction vessel; an analyzing unit for analyzing a reaction solution of a reagent and a sample;
In an automatic analyzer equipped with a storage unit for storing the measurement order of the measurement items and an analysis control means for instructing the measurement order based on the storage, the items to be analyzed are determined by the dispensing probe in an amount required for the analysis. In addition, a sample dispensing method that aspirates an extra sample and discharges only the amount required for analysis, and aspirates only the amount required for analysis by the dispensing probe and pushes out the sucked sample with a liquid other than the sample. The sample dispensing method to be discharged is divided into two groups, and the storage unit stores the measurement order in which the same dispensing method is continuously performed.

[0014]

(Embodiment 1) Hereinafter, an automatic analyzer according to the present invention will be described with reference to the drawings. FIG. 2 shows a configuration example of the automatic analyzer according to the embodiment of the present invention. Sample disk 2 for setting the container containing the liquid sample
01, a sample dispensing probe 20 for partially collecting (dispensing) a liquid sample from a container set on the sample disk 201
4, a reaction cell 211 for reacting a liquid sample with a reagent and a reaction disk 206 as a holder thereof, reagent disks 202 and 210 for setting a reagent corresponding to a measurement item, reagent probes 203 and 209 for dispensing a reagent, a reaction Cell 2
A stirring mechanism 208 for stirring the reaction solution in order to stabilize the reaction between the liquid sample dispensed in 11 and the added reagent;
Washing mechanism 20 for sucking and washing waste liquid in the reaction cell
7, and control of these mechanisms and analysis.
Further, the set of the liquid sample may be a sample rack instead of the sample disk. Regarding the reagent dispensing movement mechanism, the reagent probe
The plane movement by the Y mechanism may be used.

The liquid sample dispensing method of the automatic analyzer having the above configuration will be described with reference to FIG. 3 which is an enlarged view of the sample dispensing mechanism, and FIGS. 4 and 5 which are explanatory diagrams for explaining the sample dispensing. explain. FIG. 4 shows a sample dispensing method when the liquid sample dispensing amount is large. First, in order to prevent the sample from coming into contact with the probe and the system water 403 in the channel, the segmented air 402 is sucked. Next, the sample probe 204 is rotated and lowered on the sample container 406, the tip of the probe is dipped into the sample by several mm by the liquid level detection function, stopped, and the sample is sucked. At this time, in addition to the measurement sample 405, a dummy sample 404 for eliminating the influence of thinning of the sample is sucked. Thereafter, the sample probe rises, rotates on the reaction cell 211, moves down to the bottom of the reaction cell, and discharges the sample. At this time, only the measurement sample 405 is discharged on the reaction cell. Finally, the cleaning water is moved to the cleaning tank 301, and the cleaning water is sprayed on the outer wall of the probe.
Is washed by. Dispensing volume is 2μl by this dispensing method
Dispensing can be performed to a certain degree while maintaining the accuracy required for analysis. However, in the case of a small amount of less than 2 μl, the sample s _out1 attached to the outer wall of the probe is taken into the reaction cell after the sample is aspirated, and the sample is returned after the discharge s _out2.
As a result, the influence of the variation on the dispensing accuracy increases.

Next, FIG. 5 shows a dispensing method for ensuring accuracy in a minute amount dispensing. First, the segment air 402 is sucked, and only the measurement sample 404 is sampled accurately. next,
Before discharging the sample into the reaction cell 211, the outer wall of the nozzle is washed in the washing tank 301 to wash away the carry-in sample 407. Thereafter, by discharging the measurement sample while pushing it out with the system water 401 on the reaction cell 211, the nozzle tip is washed with the system water, and the carry-out amount of the measurement sample is reduced as compared with the dispensing method using the dummy sample. . The removal of the sample adhering to the outer wall after the suction of the liquid sample may be performed by jetting a gas such as air or by suction using a vacuum pump instead of washing with water in the washing tank 301. The ejection of the liquid sample at the time of ejection may be performed by using a physiological saline solution, a diluent, or the like instead of the system water (pure water). The system water for extruding the sample has the purpose of preventing the sample from being left undischarged in the nozzle in addition to the cleaning effect of the nozzle tip, and the amount of water may be constant or variable according to the dispensed amount. With this dispensing method, it is possible to secure the accuracy required for analysis even when dispensing a small amount of sample. However, as compared with the dispensing method using a dummy sample, the entire sequence is complicated and the time required per cycle becomes longer. As an example, a single sample dispensing mechanism and an automatic analyzer having a reaction line configuration as shown in FIG.
In the case of a zero-test device, the time required for one cycle is calculated to be within 4.5 seconds, and the sample dispensing sequence must be completed within 4.5 seconds. Table 1 shows an example of the sample dispensing sequence.

[Table 1] This means that when the maximum sample dispensing volume is 30 μl, (a) shows the operation required time by the dispensing method using a dummy sample,
(B) Time required for dispensing operation of 30 μl in the dispensing method by extruding and discharging with system water after cleaning the outer wall after aspirating the sample, and (c) requires dispensing operation of 2 μl in the same dispensing method as (b) Time. In (a), the total operation time is 4.
Although it is 3 seconds, (b) is 5.05 seconds, which is less than 4.5 seconds, which is less than one cycle time. This is because an operation not included in (a) is added in the suction of the system water, the washing after the suction of the sample, and the like. However, if the maximum dispensing amount in the method of washing after sample suction and pushing and discharging with system water is set to 2 μl, the suction and discharge time of the sample can be greatly reduced, so that the total operation time is 4.25 seconds, and one cycle time 4 .5 seconds. 2μ from the viewpoint of ensuring accuracy
With a sample dispensing amount of 1 or more, there is no problem in the dispensing method using a dummy sample.

Therefore, as an embodiment of the sample dispensing in the apparatus, a dispensing method using a dummy sample is used for a liquid sample dispensing item of 2 μl or more, and a liquid sample dispensing item of less than 2 μl is used for a sample dispensing item after aspirating the sample. By adopting a system of washing and extrusion discharge using system water, it is possible to provide an automatic analyzer that can cope with even a small amount of dispensing and maintains a processing ability.
In addition, since the limit of accuracy in the dispensing of the dummy sample differs depending on the shape of the dispensing probe, the dispensing amount for switching to the minute sample dispensing method may be 2 μl or more depending on the limit. .

In this manner, by setting the two dispensing methods to be selectively used in accordance with the required amount of the sample, the dispensing accuracy required for the analysis can be ensured regardless of the dispensing amount and the analysis can be performed. An automatic analyzer that can reduce the time required for the automatic analysis is provided. The proper use of the dispensing method can be realized by using the same dispensing probe and changing the settings of the sample suction amount and the system water pushing amount by software controlling the dispensing probe. Further, a configuration may be employed in which dispensing probes whose shapes are optimized according to the dispensing method are separately provided. (Example 2) Next, a brief description will be given of a method of sample pre-dilution dispensing as another sample dispensing method. Pre-dilution dispensing of a sample means dispensing a sample that has been diluted to a known magnification in advance, for example, because the measurement range of the measurement item is over. As shown in FIG. 3, the reaction cell 2 of the sample dispensing probe 204
11 is the normal sample discharge position 3
05 and two diluted sample suction positions 304. In the sample pre-dilution sequence, a sample is first dispensed to a normal sample discharge position 305 on a reaction cell by a dummy sample dispensing method. This dispensing method may be a minute dispensing method by washing the outer wall after aspirating the sample and pushing out and discharging the system water. Subsequently, the reaction disk is rotated and the system water or the diluting liquid is added by the reagent probe 203 at the reagent discharge position, and stirred by the stirring mechanism 208 to obtain a diluted sample. In this embodiment, since the rotation of the reaction disk is advanced by one rotation + 1 position in one cycle, the reaction cell 211 into which the diluted sample has been dispensed in FIG. 2 stops at the diluted sample suction position 304 after seven cycles. . During this stop, the sample is aspirated by the sample dispensing probe and discharged to the discharge position 305 of the empty cell in the next cycle. At this time, since the component concentrations of the undiluted sample and the diluted sample are different, in order to dispense the diluted sample from the diluted sample suction position 304 to the discharge position 305, it is necessary to clean the inside of the sample dispensing nozzle 306 before dispensing. Is performed, dispensing is performed by a dispensing method using a dummy sample or a minute dispensing method.

As described above, in the automatic analyzer having a plurality of sample dispensing methods such as the dispensing method according to the sample dispensing amount and the dispensing method using sample pre-dilution, the total consumption of the liquid sample is An embodiment of an efficient registration method of the measurement order of the analysis items for quickly presenting the test result of the same sample without reducing the processing capacity and reducing the processing capacity will be described with reference to FIGS.

In order to reduce the consumption of the liquid sample from the viewpoint of the order of registration of the analysis items, it is necessary to reduce the consumption of the dummy sample. In the case where the dispensing by the dummy sample is continued for the same sample, the suction of the dummy sample is required only once at the first time, and thereafter, only the measurement sample should be sucked and discharged. However, if items with a dummy sample and items with a small amount or a sample with pre-dilution are mixed, it is necessary to wash the inside of the nozzle with the internal system water once, and then aspirate the dummy sample again. Is required. Therefore, unnecessary dummy sample consumption can be suppressed by separately dividing items that require a dummy sample or items that do not need to use the internal cleaning each time and other items and collectively dispense them before or after each. . Accordingly, in order to reduce the amount of reagent used, first, grouping is performed according to the dispensing method according to the required amount of the sample of the analysis item.

Next, a problem concerning the processing capacity is as follows.
This is due to overlap of reagent probes and avoidance of carryover. The overlapping of the reagent probes will be described by taking an automatic analyzer having the configuration of FIG. 2 as an example. In the apparatus of FIG. 2, the reagent probe 203 is R1 (first reagent), the reagent probe 20
9 discharges R2 (second reagent) and R3 (third reagent). R1, R2, and R3 are reagents added at regular intervals after dispensing the liquid sample depending on the analysis method, and the combinations thereof are various. At this time, the reagent probe 203
Is no problem because only R1 is ejected.
In step 09, the reagents of R2 and R3 are ejected. Therefore, if the ejection timing of R2 of one measurement item and the ejection timing of R3 of another measurement item overlap, whether the registration order of the measurement items is changed or an empty cell is used. Measures are required.
In addition, avoiding carryover means that the reagents or reaction solution used for analysis may be carried by the reagent probes 203, 209, the stirring rod 208, the reaction cell 211, and the like for the next analysis item. This is a function to prevent the analysis of the combination in order to affect the measurement result. Specifically, methods for avoiding combinations that affect the analysis result are the problems of replacing the measurement order of analysis items with other items, and performing cleaning of probes and cells to be carried over. There is a method of registering as an empty cell until the combination is broken. However, even if reagent probes overlap and carry-over is avoided, washing the probe or emptying cells delays the analysis by one cycle or more, and reduces the processing capacity. It is desirable to respond. Therefore, an efficient order registration of analysis items in terms of processing capacity in the apparatus configuration of FIG. 2 will be described. In order to consider the overlap of the reagent probes 209 in the dispensing of the reagents R2 and R3, they are divided into Gr.A to Gr.C depending on the presence or absence of R2 and R3 as shown in FIG. Then, since there is a high possibility that the probe will overlap, R2
It is registered so that certain items of the reagent and the R3 reagent are preferentially analyzed. At this time, if the probes overlap, the items of the next group are registered first. If there are no items that can be registered in the same sample, empty cells are used until there is no overlap.

Carryover check is also performed at the time of registration for each item in the group. If there is a problem, the order of registration is first changed in the group, and if registration in the group is difficult, registration in the next group is performed. Register the item first. When the registration order is changed by the carry-over check, the overlapping of the reagent probes is checked again. In the case of carry-over avoidance, if registration is not possible due to replacement of item order in the same sample, empty cells or carry-over washing is performed.

In order to minimize the data output time of the same sample, it is effective to register the measurement order in the order of longer reaction time.

An example of the registration order of the measurement items according to the above basic rules is shown together with the flowchart of FIG.
First, a measurement request item for a liquid sample is input (Step 2, hereinafter S2). The sample dispensing method is divided into a dispensing method using a dummy sample and another dispensing method (dispensing method of nozzle wall cleaning after sample suction and discharge by water extrusion, dispensing method by sample pre-dilution, etc.) S3) The maximum analysis time in the two groups is compared (S4). this is,
This is because the analysis data of the same sample is output quickly by analyzing the group having the maximum analysis time first.

Subsequently, the R2 and R3 reagents are divided into subgroups depending on whether or not they are dispensed (S5). The main group including the item with the longest reaction time is prioritized, and the subgroups are registered in the order of the longest reaction time in the subgroup with the priority Gr.A>Gr.B> Gr.C (S6). These examples are illustrated in FIG. Each item name is expressed in Greek letters, and the reaction time is shown in parentheses. In this example, 15 minutes of the item β is the maximum analysis time, and the group of sample dispensing items without dummy has priority. After that, it is divided into sub-groups (Gr. A, Gr. B, Gr. C) and registered in the sub-group in order of the reaction time. Further, when each item is registered, a check is made as to whether reagent probes overlap and carry-over is avoided (S7). If there is a problem, the order is changed as described above or an empty cell is set (S8). When the registration of all the measurement item sequences is completed (S9), the process ends and the sample dispensing / analysis is started (S10).

In this embodiment, the sample pre-dilution item is included in items other than the dummy dispensing item. However, if the number of pre-dilution items is 1 to 3, the sample pre-dilution item is replaced with the dummy sample dispensing item. By inserting at the end, it is not necessary to aspirate the dummy sample again for dispensing the base sample for preparing the diluted sample, so that the liquid sample consumption is suppressed and effective, so such a configuration may be adopted. .

[0027]

According to the present invention, since the consumption of a liquid sample can be reduced, it is possible to cope with the analysis of a small amount of sample such as a pediatric sample, and the consumption of a reagent is minimized. Thus, the analysis time can be reduced.

[Brief description of the drawings]

FIG. 1 is a flowchart of an embodiment of the present invention.

FIG. 2 is a configuration diagram of an automatic analyzer according to an embodiment of the present invention.

FIG. 3 is a partially enlarged view of a liquid sample dispensing probe and its surroundings.

FIG. 4 is an explanatory diagram showing a sample dispensing operation using a dummy sample.

FIG. 5 is an explanatory view showing a sample dispensing operation by cleaning the outer wall of a nozzle and extruding water after suctioning the sample.

FIG. 6 is a diagram showing grouping by reagent dispensing R2 and R3.

FIG. 7 is a schematic diagram summarizing an embodiment of the present invention.

[Explanation of symbols]

201: sample disk, 202: reagent disk 1, 20
3 ... reagent dispensing probe 1, 204 ... sample dispensing probe,
205 photometer, 206 reaction disk, 207 reaction cell washing mechanism, 208 stirring mechanism, 209 reagent dispensing probe 2, 210 reagent disk 2, 211 reaction cell,
301: sample dispensing probe cleaning tank, 302: sample disk outer suction position, 303: sample disk inner suction position, 3
04: diluted sample suction position (reaction cell), 305: sample discharge position (reaction cell), 306: sample nozzle, 401: sample nozzle cross section, 402: segmented air, 403: system water, 404: dummy sample, 405: measurement Sample, 406 ...
Sample cup cross section, 407: sample attached to nozzle outer wall after sample suction, 408: sample attached to nozzle outer wall after sample ejection, 50
1 ... Washing water from washing tank, 601 ... Second reagent present, 3rd
Group of analysis items with reagents, 602: group of analysis items without second reagent, no third reagent, 603: group of analysis items without second reagent, 701: group of all measurement request items, 702: no dummy 703: a group of sample dispensing items using a dummy; 704: a subgroup divided by the presence or absence of dispensing of each reagent; 705: registration of the measurement order of measurement request items;
01: One cycle operation required time for sample dispensing using a dummy sample (sample dispensing amount: 30 μl), 802: One cycle for sample dispensing using nozzle outer wall cleaning after sample aspiration and extrusion discharge with system water Required operation time (sample dispensing amount: 30 μl), 803: Required time for one cycle operation in sample dispensing using nozzle outer wall cleaning after sample aspiration and extrusion by system water (sample dispensing amount: 2 μl).

 ──────────────────────────────────────────────────続 き Continuing from the front page (72) Inventor Tomonori Mimura 882-Chair, Oaza-shi, Hitachinaka-shi, Ibaraki Pref. Within the Measuring Instruments Group of Hitachi, Ltd. F-term in the Hitachi Measuring Instruments Group (reference) 2G058 AA05 CB05 CD04 CE08 EA02 EA04 EA11 ED03 ED12 ED21 FA02 FB06 FB07 GA03 GE03

Claims (7)

[Claims] In an automatic analyzer having a dispensing probe for dispensing a liquid sample into a reaction vessel and an analyzer for analyzing a reaction solution of a reagent and a sample, the liquid sample of the liquid sample is analyzed in accordance with an analysis item of the sample. The dispensing method, a sample dispensing method in which a sample is sucked extra than the amount required for analysis by the dispensing probe and only the amount required for analysis is discharged, or the sample only for the amount required for analysis by the dispensing probe is used. An automatic analyzer characterized by being configured to use one of a sample dispensing method in which aspirated and aspirated samples are extruded with a liquid other than the sample and discharged. 2. An automatic analyzer comprising: a dispensing probe for dispensing a liquid sample into a reaction container; and an analyzer for analyzing a reaction solution of a reagent and a sample. The dispensing method of the sample is either a sample dispensing method in which the sample is sucked extra than the amount required for analysis by the dispensing probe and only the amount required for analysis is discharged, or only the amount required for analysis by the dispensing probe. An automatic analyzer characterized by using one of a sample dispensing method in which a sample is sucked and the sucked sample is extruded with a liquid other than the sample and discharged. 3. An automatic analyzer having a dispensing probe for dispensing a liquid sample into a reaction container and an analyzer for analyzing a reaction solution of a reagent and a sample, wherein the dispensing method of the liquid sample is dispensed. When the amount of the sample is equal to or more than a predetermined amount, the dispensing probe uses a sample dispensing method in which the sample is aspirated extra than the amount required for analysis and only the amount required for analysis is discharged. If the amount of the sample to be performed is less than a predetermined amount, the dispensing probe aspirates only the amount of the sample required for analysis, and pushes out the aspirated sample with a liquid other than the sample to discharge the sample. An automatic analyzer characterized in that it is configured to be used depending on the analysis item of a sample to be used. 4. A dispensing probe for dispensing a liquid sample into a reaction container, an analysis unit for analyzing a reaction solution of a reagent and a sample, a storage unit for storing a measurement order of measurement items, and a measurement order based on the storage. And an analysis control means for instructing the sample to be analyzed. The sample to be analyzed is obtained by aspirating a sample more than the amount required for analysis by the dispensing probe and discharging only the amount required for analysis. The same dispensing is performed by dividing the sample into two groups: a dispensing method, and a sample dispensing method in which only the amount required for analysis is aspirated by the dispensing probe, and the aspirated sample is extruded with a liquid other than the sample and discharged. An automatic analyzer characterized by storing a measurement order in which the methods are continuously performed in the storage unit. 5. The automatic analyzer according to claim 4, wherein, among the grouped analysis items, a measurement order in which the analysis item having a long analysis time is dispensed first is stored in the storage unit. An automatic analyzer characterized by the following. 6. The automatic analyzer according to claim 4, wherein the dispensing probe aspirates a sample more than an amount required for preparing a diluted sample and discharges only an amount required for preparing a diluted sample. After dispensing the sample into the reaction vessel by the sample dispensing method, if there is a measurement item based on the diluted sample prepared to the required magnification by adding a liquid for dilution, the amount required for analysis by the dispensing probe is After the dispensing using the sample dispensing method that aspirates an extra sample and discharges only the amount required for analysis is completed, the measurement order is stored in the storage unit so that the measurement items are continuously performed. An automatic analyzer characterized by the following. 7. The automatic analyzer according to claim 1, wherein the dispensing probe aspirates only an amount of the sample required for analysis, and extrudes the aspirated sample by extruding it with a liquid other than the sample. An automatic analyzer, wherein the dispensing probe is washed after the sample is aspirated and before the sample is ejected.