JP2002153259A - Collector for floating microorganism and dust - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a collector which is used for collecting floating microorganisms and dust and can readily be used at any place without needing an electric source. SOLUTION: This collector for collecting the floating microorganisms and the dusts, characterized by disposing a receiver for loading a container such as a laboratory dish in a chamber, disposing an outer air intake port above the receiver, attaching a nozzle for jetting the outer air taken from the suction port toward the laboratory dish or the like, connecting a suction device for evacuating the chamber to the evacuation port of the chamber, and disposing a spring power device as a source for driving the suction device.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a device for collecting floating bacteria and dust which does not require a power source and which is provided with a mainspring power unit.

[0002]

2. Description of the Related Art Conventionally, as an apparatus for inoculating fungi in an agar medium, an apparatus comprising a combination of a means for perforating an agar medium and a means for applying fungi to a groove opened by the perforating means, or an injection needle is used. There is a configuration that can be directly planted on an agar medium. However, in the former, two kinds of operations, that is, the operation of perforating the agar medium and the operation of applying the fungi, had to be performed in order. In addition, in both cases, in order to inoculate fungi on the agar medium, the planting means such as a perforating means, a coating means, and an injection needle had to be raised and lowered many times, and the work was inefficient. Desirably, the planting means should be able to plant fungi deep into the agar medium without touching the agar medium. In order to satisfy the seemingly contradictory requirements, it is conceivable to employ means for spraying fungi on the agar medium. The spraying method is efficient because fungi can be inoculated deeply without touching the agar medium.

[0003] As an example of this configuration, for example, a so-called Andersen type floating bacteria collecting apparatus can be used. In this apparatus, a tray for placing a petri dish containing an agar medium is placed in a casing, a fan for guiding outside air into the casing and then exhausting the air is connected to an exhaust port of the casing, and the casing is provided with an outside air. A suction port is provided, and a perforated plate having a plurality of small holes is attached to the suction port so that the outside air passing through the perforated plate hits the agar medium in a petri dish. Further, as another example, as disclosed in JP-A-6-160250, a saucer for mounting a petri dish containing an agar medium is rotatably provided in a chamber by a driving device, and a negative pressure is applied to the inside of the chamber for negative pressure. A suction device is connected to the air vent of the chamber, a suction port for outside air is provided in the chamber, and a nozzle for injecting the outside air sucked from the suction port toward the agar medium of the petri dish is attached to the inside of the chamber to collect floating bacteria. Collector can be raised.

[0004] The above-mentioned collecting device is of a type in which floating bacteria are inoculated on an agar medium. In some cases, the cells are cultured on the agar medium as it is, or the agar having the bacteria inoculated is taken out with a dropper or the like and washed. In some cases, however, the operation of putting the liquid into ATP is performed. In the latter case, the work was laborious and time-consuming. On the other hand, there is a type in which fungi are directly applied to distilled water or special cleaning water.
P can be collected and supplied. The purpose of collecting airborne bacteria is to count airborne bacteria mainly in the air in a general environment such as an office or in a clean room.

[0005]

Among the above-mentioned devices for collecting suspended bacteria, the former so-called Andersen-type device for collecting suspended bacteria has an advantage that the fan can be driven by a small motor, so that the whole can be reduced in size. However, there is a problem that power must be supplied from an outlet or a battery is required. The latter device for collecting suspended bacteria has a suction device and a chamber integrally provided. Generally, a caster is attached to the bottom of the suspended bacteria collecting apparatus, and the caster is moved from a place in an office or a clean room where a sample is desired to be taken. However, since power must still be supplied from the outlet, there is inconvenience in having to search for the outlet and to remove and insert the plug every time the user moves. In addition, conventional airborne bacteria collection devices cannot be used in areas where supply is not available, and it is difficult to obtain batteries such as those in developing countries and remote areas, even if a battery-driven device is prepared. It is difficult to use in areas where battery is expensive or expensive. Further, in the battery, there is a problem that the power generation ability of the battery changes with time, and the performance changes between the first use and the later use, and the liquid leaks.

The present invention is intended to solve such a problem and to provide a device for collecting floating bacteria and dust which does not require a power source, can be easily used anywhere, and has a constant collecting capacity. is there.

[0007]

SUMMARY OF THE INVENTION The object of the present invention is to provide a receiving portion for mounting a petri dish or the like in a chamber, providing an outside air suction port above the receiving portion, and sucking the air from the suction port. A nozzle for injecting the outside air toward the petri dish is attached, a suction device for making the inside of the chamber a negative pressure is connected to the exhaust port of the chamber, and a mainspring power unit is provided as a drive source of this suction device This is achieved by a device for collecting floating bacteria and dust.

When the mainspring power device is operated to urge the mainspring, a rotational force is generated by the force when the mainspring is turned back. This rotational force drives the suction device to make the inside of the chamber a negative pressure, so that outside air is sucked from the suction port and vigorously passes through the nozzle, and the outside air is blown onto a petri dish or the like placed on the receiving portion. At this time, by forming an agar medium on a Petri dish, etc., the outside air containing airborne bacteria and dust is blasted vigorously onto the surface of the agar medium, and the airborne bacteria and dust mixed in the external air go deep into the agar medium. Planted.

[0009] The mainspring power unit is connected to the suction device and can be manually turned to wind it, so that this collection device does not require any power source such as a battery. A suction device that uses various pistons and fans can be used.
Which of them is adopted is arbitrary. Further, the speed of the suction device can be increased by interposing a speed increasing gear train between the mainspring power device and the suction device.

[0010] Next, in the case where the nozzle is constituted by an arrangement of pinholes, the inhaled outside air is vigorously blown from the eyes of the pinhole toward the agar medium. The shape of the nozzle is arbitrary, such as a long and thin hole. Particularly in the case of a pinhole, fungi can be planted vigorously. Therefore, by appropriately designing the number and arrangement of the pinholes, it becomes possible to increase the number of colonies and count and measure.

When the pinholes are arranged radially from the center to the periphery of the nozzle, colonies can be formed radially. If the pinholes are arranged in a lattice, colonies can be formed in a lattice. In addition, the arrangement pattern of the pinholes does not necessarily have to be exactly arranged in one line.

Next, in the case where the suction device has a filter device capable of filtering fungi at its discharge portion, the air discharged from the suction device can be cleaned by the filter device. . Whether to filter only fungi or even dust is an optional design matter. However, in case you only want to inoculate fungi without changing the original outside air condition, or if you do not want to bring the atmosphere of the room where you use the filter to the next room, etc. It can be detachably attached. Alternatively, the filter can be replaced with an air purifier.

Next, as a drive source of the suction device, a drive device by a motor connected to a generator driven by a mainspring may be provided. Since the power source of the motor was a problem in the first place, providing the power generator driven by the mainspring eliminates the worry about the power source. The generator is driven by the mainspring to generate and rectify power, and supplies this power to the motor. The motor is connected to the suction device via a speed increasing gear train, and the inside of the chamber becomes negative pressure by driving the suction device.

Next, in a gear provided with a stopper connected to the mainspring power unit, the operation of the gear can be stopped or operated by hanging or removing the stopper. Device ON /
OFF can be controlled. In the case where the mainspring was not provided, the mainspring was manually wound up and the hand was released.

Next, a handle was attached to the container containing the chamber. Therefore, it is easy to move from a place where a sample is to be taken to a place only by grasping a handle, and it is also convenient to perform sampling while changing the height position.

When connecting the rotary shaft of the mainspring power unit to the suction device, the connection is made by mechanical contact, or when the connection is made in a mechanically non-contact state using a hydraulic joint or a magnetic joint. There is. In order to prevent the mainspring from being overwound, a recess is provided around the wall of the container that holds the mainspring, and the outer end of the mainspring is locked, and this locking state is released when a certain level of winding force is applied Being
The outer end of the mainspring may be slid into a recess around the wall of the container and may not be rolled any further.

[0017]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described with reference to the drawings, but the present invention is not limited to these embodiments.

FIGS. 1 to 3 show a first embodiment of the present invention. The handy type collector 1 has a head 2 and
The suction drive unit 3 having the handle 30 and the base 4 are vertically stacked. The head 2 includes a bowl-shaped suction port 20 and a perforated nozzle 21 provided below the suction port 20. Further, an intake passage 23 for sending the intake air having passed through the multi-hole nozzle 21 to the suction drive unit 3 at the next stage is provided. Head 2
Is inserted into the upper end of the suction drive unit 3 described below.

The suction drive unit 3 includes a hand winding wheel 31 attached to a rotating shaft (not shown) of the mainspring 32 for winding the mainspring 32, a speed increasing gear train 33 attached to the rotating shaft of the mainspring 32, A turbo fan 35 attached to an output shaft 34, and makes the turbo fan 35 desired in an intake passage 36 continuing from the preceding intake passage 23;
5 is connected to an exhaust port 37 opened on the side wall of the suction drive unit 3. Note that the hand-wound wheel 31 is exposed from the bottom of the suction driving unit 3, and the notch 3 is formed on the side wall of the suction driving unit 3 so as to contact the hand-wound wheel 31 and wind it up.
8 was formed. The notch 38 is integrated with a notch 40 provided on the upper edge side wall of the base 4 described below.
At the top of the suction drive unit 3, a mounting table 39 for placing the petri dish S in close proximity to the bottom of the multi-hole nozzle 21 is provided so as to protrude upward from the top.

The base 4 is configured so that the suction drive unit 3 and the hand-wound wheel 31 can be fitted thereon. The base 4 is recessed by the cutout 38 and the cutout 40 provided on the upper edge side wall. Is formed, and the hand-wound wheel 31 is positioned in the depression, so that the hand-wound wheel 31 can be wound up with a fingertip.

An injection hole 22 is formed on the entire surface of the multi-hole nozzle 21 as shown in FIG. 2, and its cross-sectional shape has a horn shape reaching the outlet diameter φ as shown in FIG. However, the shape is not limited to this shape, and the inlet and the outlet may be straight with the same diameter. Here, it is assumed that φ is 0.8 mm, and that 500 nozzle holes 22 are opened in the multi-hole nozzle 21. The injection holes 22 on the entire surface are arranged in a lattice.

Now, the usage of the collector 1 of this embodiment will be described. When the head 2 is removed, the mounting table 3 is placed from the top of the suction drive unit 3.
Since 9 is in a protruding state, for example, a Petri dish S on which an agar medium is formed is placed here. When the head 2 is fitted to the suction drive unit 3, the multi-hole nozzle 21 is located immediately near the agar medium of the petri dish S. When the preparation is completed, the hand winding ring 31 exposed from the side wall surface of the collector 1 at the cutouts 38 and 40 is picked up by the index finger and the thumb, and the mainspring 32 is wound. When the force applied to the hand-wound wheel 31 is removed after the winding has reached the limit, the force of the mainspring 32 to rewind is increased by the speed increasing gear train 33.
, And transmitted to the turbo fan 35 via the output shaft 34, and the turbo fan 35 rotates vigorously.

The rotation of the turbo fan 35 causes the intake passages 23 and 36 to have a negative pressure.
0, the outside air is sucked in,
From the petri dish S to the agar medium. With this momentum, fungi contained in the outside air are planted on the agar medium in the petri dish S placed on the mounting table 39.
This is because fungi are not simply attached to the surface of the agar medium, but are implanted from the surface of the agar medium into the interior by the force of external air. Thereafter, the outside air is exhausted from the exhaust port 37.

FIG. 4 shows an embodiment of the multi-aperture nozzle 24 in which the injection holes 22 of the multi-aperture nozzle 21 are arranged in a grid pattern. 22 are arranged radially. Various other arrangements are possible depending on the application.

Next, FIG. 5 shows an embodiment in which the suction drive section 3 is provided. With this filter 5, the air exhausted from the exhaust port 37 can be cleaned. Whether to filter only fungi or dust is an optional setting. Note that the filter 5 can be made detachable.

Next, a second embodiment of the present invention will be described with reference to FIGS. In the first embodiment described above, the hand winding wheel 31 is provided to wind the mainspring 32 of the suction drive unit 3. On the other hand, according to this embodiment, the string 60 wound around the pulley 6 attached to the rotating shaft 300 of the mainspring 32 is pulled out to the outside through the drawing hole 301 formed in the side wall of the suction drive unit 3, To which a ring 61 was attached.

Therefore, when the finger is hung on the ring 61 and the string 60 is pulled, the mainspring 32 is wound. When the finger is released from the ring 61 when the ring is wound up to the limit, the force of unwinding the mainspring 32 increases the speed increasing gear train 33. , And transmitted to the turbo fan 35 via the output shaft 34, and the turbo fan 35 rotates vigorously. In addition, as the mainspring 32 rewinds, the pulley 6 winds up the string 60, and the ring 61 returns to the vicinity of the original drawing hole 301.

Next, a third embodiment of the present invention will be described with reference to FIG. In this embodiment, the generator 7 is attached to the output shaft 34 of the speed increasing gear train 33, and this electrical output is supplied to the motor 70 via the lead wire 71. A turbo fan 35 is attached. Therefore, there is no direct connection between the output shaft 34 of the speed increasing gear train 33 and the rotating shaft 72 of the motor 70.

According to this rotation transmitting mechanism, the mainspring 32
Is temporarily converted into an electrical output by the generator 7, thereby driving the motor 70 and the turbo fan 3.
5 is rotating.

Next, a fourth embodiment of the present invention will be described with reference to FIG. In this embodiment, the magnetic disk 8 is mounted on the output shaft 34 of the speed increasing gear train 33, a magnetic cup 80 is provided so as to surround the magnetic disk 8, and the turbo fan 35 is mounted on a rotating shaft 81 of the magnetic cup 80. Consisting of Therefore, in this embodiment as well, there is no direct connection between the output shaft 34 of the speed increasing gear train 33 and the rotation shaft 81 of the turbo fan 35, but the rotation of the output shaft 34 Motion, and the magnetic cup 80 produces a rotational motion in a magnetically dragged form,
As a result, the rotation of the turbo fan 35 is linked. That is, the set of the magnetic disk 8 and the magnetic cup 80 is a magnetic coupling.

The mainspring 32 has a mainspring portion (not shown) built in the case, and a ratchet hole 302 is formed in the outer periphery of the case, while the outer end of the mainspring portion is closed. By bending outward, a hook is formed to be locked in the ratchet hole 302 (not shown). Accordingly, if excessive rotational force is applied to the mainspring 32 by winding the handwheel 31 excessively, a hook (not shown) comes off the ratchet hole 302 and the hook is locked in the ratchet hole 302 when the tension of the mainspring is released. Will be done. That is, it does not mean that the mainspring 32 is excessively wound.

Next, a fifth embodiment of the present invention will be described with reference to FIG.
This will be described with reference to FIG. The configuration of the collector 1 ′ of this embodiment is similar to the configuration of the collector 1 of the first embodiment, but is characterized in that a switch 9 is provided on the base 4. This switch 9 turns on / off a ratchet mechanism (not shown).
A ratchet pawl (stopper) for turning off and engaging with a ratchet gear attached to the hand-wound wheel 31
For engaging and disengaging the gear from the gear.

Therefore, in the notches 38 and 40,
When the hand-wound ring 31 exposed from the side wall surface of the collector 1 ′ is picked up by the index finger and the thumb and wound, the mainspring 3
At this time, even if the hand is released from the hand-wound wheel 31 by the action of the ratchet mechanism, the mainspring 32 is wound.
Never rewind. Similarly, even if the hand-wound wheel 31 is wound to the limit and then the force applied to the hand-wound wheel 31 is removed, the mainspring 32 does not similarly rewind. Next, when the switch 9 is pressed as necessary, the ratchet mechanism is released, and the urging force of the mainspring 32 is increased by the speed increasing gear train 33 and transmitted to the turbo fan 35 via the output shaft 34, The turbo fan 35 rotates vigorously.

The present invention is not limited only to the above-described embodiments and examples. For example, the material and shape of the casing of the collector are arbitrary, and the head 2, the suction drive unit 3, the base It is not necessary to divide the structure into the base 4 and a mechanism other than the hand-wound ring 31 may be adopted for winding the mainspring 32, and the form of the nozzle is not particularly limited to the embodiment. The same applies to the configuration of the suction device. The handle 30 described above may or may not be attached. In the case where a stopper for temporarily stopping rewinding of the mainspring 32 is attached and a handle is provided, a design in which a switch for the stopper is provided on the handle is possible. The type of adherend such as a petri dish on which bacteria and dust are adhered and the type of culture medium are irrelevant to the configuration of the present invention.

[0035]

As described above, according to the present invention, a receiving portion for mounting a petri dish or the like is provided in the chamber, an external air intake port is provided above the receiving portion, and the external air sucked from the intake port is supplied to the petri dish. A nozzle for injecting toward the etc. is attached, a suction device for making the inside of the chamber a negative pressure is connected to the vent of the chamber, and a spring power unit is provided as a drive source of this suction device. This is a device for collecting floating bacteria and dust.

As a result, a power source is unnecessary and can be easily used anywhere, thereby achieving the intended purpose.

[Brief description of the drawings]

FIG. 1 is a schematic sectional view of a first embodiment.

FIG. 2 is a plan view of the multi-hole nozzle 21 of the embodiment.

FIG. 3 is an explanatory view of an injection hole 22 of a multi-hole nozzle 21.

FIG. 4 is a plan view of a multi-hole nozzle 24 according to another embodiment.

FIG. 5 is a schematic cross-sectional view of the suction drive unit 3 when a filter 5 is attached.

FIG. 6 is a side view of a suction drive unit 3 according to a second embodiment.

FIG. 7 is a schematic sectional view of the embodiment.

FIG. 8 is a schematic diagram of a rotation transmission mechanism according to a third embodiment.

FIG. 9 is a schematic diagram of a rotation transmission mechanism according to a fourth embodiment.

FIG. 10 is a side view of the fifth embodiment.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Collector 1 'Collector 2 Head 20 Suction port 21 Perforated nozzle 22 Injection hole 23 Intake passage 24 Perforated nozzle 3 Suction drive part 30 Handle 31 Hand-wound wheel 32 Mainspring 33 Speed-up gear train 34 Output shaft 35 Turbo Fan 36 Intake passage 37 Exhaust port 38 Notch 39 Mounting table 300 Rotating shaft 301 Extraction hole 302 Ratchet hole 4 Base 40 Notch 5 Filter 6 Pulley 60 String 61 Ring 7 Generator 70 Motor 71 Lead wire 72 Rotating shaft 8 Magnetic disk 80 Magnetic cup 81 Rotary axis 9 Switch

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Kazuhiko Harada 1-110-12 Sakaemachi-dori, Tsurumi-ku, Yokohama-shi, Kanagawa 414 Takashima Heights 414 (72) Inventor Hideaki Ishikake 5--8-14 Minamiyukiya, Ota-ku, Tokyo F term (reference) 4B029 AA09 BB01 CC02 HA10

Claims (8)

[Claims] 1. A receiving portion for mounting a petri dish or the like is provided in a chamber, an intake port for outside air is provided above the receiving portion, and external air sucked from the suction port is jetted toward the petri dish or the like. A suction device for negative pressure inside the chamber is connected to a vent of the chamber, and a spring power unit is provided as a driving source of the suction device. Collection device. 2. The device for collecting floating bacteria and dust according to claim 1, wherein the nozzle is constituted by an array of pinholes. 3. The apparatus for collecting airborne bacteria and dust according to claim 2, wherein said pinholes are arranged radially from a central portion to a peripheral portion of the nozzle. 4. The apparatus according to claim 2, wherein the pinholes are arranged in a lattice. 5. The apparatus for collecting suspended bacteria and dust according to claim 1, wherein the suction device includes a filter device at its discharge portion capable of filtering fungi. 6. The device for collecting floating bacteria and dust according to claim 1, further comprising a driving device driven by a motor connected to the generator driven by the mainspring as a driving source of the suction device. 7. The apparatus for collecting floating bacteria and dust according to claim 1, wherein a stopper is provided on a gear connected to the mainspring power unit. 8. The device for collecting floating bacteria and dust according to claim 1, wherein a handle is attached to a container containing the chamber.