JP2002148520A - Illuminator for microscope - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an illuminator for a microscope so excellent in versatility that only a necessary optical device can be easily installed in a light projecting tube and adjusted and an optical device for changing a vertical illumination optical system such as an auxiliary lens can be also arranged and adjusted. SOLUTION: This illuminator for a microscope is used for vertical fluorescent observation to observe fluorescence emitted from a sample surface by irradiating the sample surface with illuminating light from a light source disposed in a vertical light projecting tube. In the illuminator, plural slider insertion ports having the same cross-sectional shape are formed on a plane perpendicular to the optical axis of vertical illuminating light in the vertical light projecting tube, and the optical device is moved on the plane perpendicular to the optical axis of the vertical illuminating light. Plural sliders different in the kinds of the optical device are provided to be inserted in/pulled out from the respective slider insertion ports.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an illuminating device for a microscope, and in particular, to observe fluorescence emitted from a sample (specimen) surface by irradiating illumination light to a sample (specimen) surface using Koehler illumination and a light source such as mercury. The present invention relates to an illumination device for a microscope used for observing incident fluorescence.

[0002]

2. Description of the Related Art Recently, Koehler illumination is mainly used for illumination for microscopes. In this method, a lamp, which is a light source, is projected onto the pupil position of an objective lens (rear focal position) to perform illumination without unevenness.

In most epi-illumination devices using Koehler illumination, an aperture stop (AS) is provided in the epi-illumination light pipe at a position conjugate with the above-described objective lens pupil position, and a field stop (FS) is provided at a position conjugate with the specimen surface. It is common to have an openable and closable stop called a "stop". It is important that the center of each stop coincides with the center of the illumination optical axis, and the aperture is usually adjusted in advance or provided with means for moving on the illumination optical axis.

In addition to the above-mentioned diaphragm, there is provided an insertion port through which an ND filter for adjusting illumination light intensity and a filter for changing the wavelength characteristic of illumination light can be freely inserted into and removed from the illumination optical path. There are many things. Since these filters may be roughly positioned with respect to the illumination optical axis, no adjusting means such as the above-described diaphragm is used.

[0005]

Recently, with the development of the field of biological research, the use of an epi-illumination device (epi-illumination floodlight) and the combination with a microscope main body have been various. For example, instead of combining an objective lens with a vertically moving microscope or a circular field stop with a conventional eyepiece, a T
There is a demand for a rectangular shape according to the light receiving element of the V-camera or an arbitrary shape according to the pinhole aperture and the observation range.

In the former case, a mechanism for moving the objective lens up and down while fixing the sample or the stage must be provided between the epi-illuminator and the objective lens. In this case, since the light source does not form an image at the pupil position of the objective lens, Koehler illumination cannot be performed, and as a result, illumination unevenness occurs.

In the latter case, it is necessary that an arbitrary stop can be arranged on the optical axis of the epi-illumination (or an arbitrary position of the user). It is anticipated that one of the important functions in the future epi-illumination device will be to solve these problems and to have excellent versatility that can easily respond to the demands of the user.

In a conventional epi-illumination device, as described in the first known example (Japanese Patent Application Laid-Open No. 8-501163), an epi-illumination device is modularized with an optical element and an aperture to be added to the epi-illumination device, and an epi-illumination light pipe ( Some epi-illumination devices are detachable from a part for guiding illumination light to a sample).

However, each unit is divided into those that require position adjustment with respect to the epi-illumination optical axis and those that do not. For units that require adjustment, an adjustment mechanism is provided in the unit. , The shapes are different. For this reason, the insertion position into the epi-illumination light pipe is restricted.

Specifically, the content disclosed in the first known example is characterized in that each adjusted module is brought into contact with a mounting accuracy surface provided on an epi-illumination light pipe and fixed in position. Each unit can be attached and detached with these epi-illumination tubes, but the units set by the manufacturer can be combined only at the position set by the manufacturer, and the user can easily place any unit at any position. Cannot be attached.

[0011] For this reason, the above-mentioned problem has not been solved. Second known example (JP-A-9-5631),
A third known example (Japanese Patent Application Laid-Open No. Hei 10-73768) discloses that an aperture stop can be inserted into and removed from an incident light projector, but this also has the same problem as described above.

A fourth known example (Japanese Utility Model Application Laid-Open No. 50-76840) discloses means for attaching and detaching and centering a condenser lens. Detachable optical elements can be attached to and detached from the microscope,
Although the present invention is similar to the present invention in that it can be centered, the present invention is characterized only by inserting a condenser lens into a predetermined slot, and provides a versatile illumination system which is the subject of the present invention. Not yet available.

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and has as its object to easily install and adjust only necessary optical elements and to change an illumination optical system such as an auxiliary lens. The present invention is to provide a microscope illuminating device excellent in versatility in which an optical element can be arranged and adjusted.

[0014]

According to a first aspect of the present invention, there is provided a microscope illuminating device for irradiating illumination light from a light source onto a sample surface, the optical device being inserted into an optical axis of illumination. A slider that holds an element and is insertable into and removable from the illumination device main body; a plurality of slider insertion openings having the same cross-sectional shape formed in the illumination device main body so as to be arranged in the direction of the illumination optical axis; A centering member that is arranged inside the insertion opening and holds the slider and adjusts the centering in a plane perpendicular to the optical axis of the illumination;
A lighting device for a microscope, comprising:

According to the first aspect of the present invention, an optical element is provided on a thin slider having the same shape, and the slider can be inserted into an illumination device main body (e.g., an incident light projection tube).
By making it movable in a plane perpendicular to the epi-illumination optical axis, not only filters but also various optical elements such as diaphragms can be inserted and adjusted at predetermined positions in the illumination system, and filters etc. can be used instead of unnecessary optical elements. Can be used to provide a lighting device that can respond to the user's request.

In order to achieve the above object, the invention corresponding to claim 2 is configured as follows. 2. The illumination device body according to claim 1, wherein the illumination device main body is a floodlight tube using Koehler illumination, and at least one of a field stop, an aperture stop, and a dimming filter is used as the optical element. 3. Of the microscope.

According to a third aspect of the present invention, an auxiliary lens for changing a projection position of an objective pupil or changing a projection magnification of a light source is used as the optical element. The illumination device for a microscope according to claim 1, wherein:

According to the third aspect of the present invention, the user can easily make some modifications to the original illumination optical system such as changing the projection position and the projection magnification of the light source by incorporating the auxiliary lens. The limitations of the lighting device itself can be reduced.

[0019]

DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment in which the present invention is applied to an epi-illumination device will be described below with reference to the drawings.

A first embodiment of the present invention will be described with reference to FIGS. 1 and 2 show the overall view of the microscope to which the present invention is applied, and a view in which an epi-illumination optical system is superimposed. FIGS. 3, 4, and 5 are cross-sectional views of the slider insertion port portion of FIG. A top view and a side view are shown. FIGS. 6 and 7 show two typical sliders inserted into the insertion slot, in which (a) is a sectional view and (b) is a front view.

First, the whole will be described with reference to FIGS. The microscope body 01 holds a stage holder 03 to which a stage 08 capable of moving a sample 09 in a plane perpendicular to the observation optical axis in the figure is fixed so as to be movable along the observation optical axis by a focusing handle 02. .

At least one illumination lens group 22, 23, 24,
An epi-illumination light pipe 200 as an illumination device main body having a position 25 is positioned and fixed with screws (not shown) or the like. An LH (lamp house) mount 21
And a lamp that holds a lamp 13 serving as a light source when the mount 14 is fitted to the LH mount 21 by an adjustment knob (not shown) so as to be movable in a plane perpendicular to the epi-illumination optical axis in the drawing. House 100 is fixed.

In the lamp house 100, a collector lens 11 composed of at least one lens for guiding the light emitted from the lamp 13 to the inside of the incident light projection tube 200 is held so as to be movable in the optical axis direction. On the side opposite to the lens 11, a concave mirror 12 is fixed at a position where the light emitted from the lamp 13 can be condensed again at the lamp emission position.

A dichroic mirror for bending the incident illumination light beam toward the objective lens 06 at the position where the incident illumination optical axis and the observation optical axis intersect in the incident light projection tube 200 and only necessary light beams from the incident illumination light are selected. Excitation filter 2
8. A plurality of lenses having at least one lens group 07 below the epi-illumination light pipe 200 are provided with an absorption filter 30 for transmitting only the fluorescent light emitted from the sample 09 and removing disadvantageous light rays. An objective lens converter 04, which holds the objective lens 06 and can arrange an arbitrary objective lens 06 on the observation optical axis, is detachably held. Here, the objective lens pupil 05 indicates the rear focal position of the lens group 07 in the objective lens 06.

A lens barrel 40 having an imaging lens 41 for imaging parallel rays passing through the objective lens 06 is detachably held above the incident light projection tube 200.
An eyepiece 50 for observing the sample 09 is provided in the lens barrel 40.
Are positioned and fixed by fitting (not shown).

The incident light projection tube 200 has at least two or more (seven in the figure) identical cross-sectional shapes including the FS 26 at a position conjugate with the sample 09 and the AS 27 at a position conjugate with the objective lens pupil 05. It has insertion openings 32 to 38, and sliders 42 to 48 having the same cross-sectional shape are held in each of the insertion openings 32 to 38 so as to be insertable and removable.

Next, the operation of the first embodiment of the illumination device for a microscope configured as described above will be described. The light beam emitted from the lamp 13 adjusted on the optical axis of the epi-illumination by an adjustment knob (not shown) is emitted by the collector lens group 11 to the light emitting tube 2.
Guided within 00. From the lamp 13 to the collector lens group 1
The light emitted in the direction opposite to the direction 1 is also used as the concave mirror 12.
Thus, the light is guided to the light emitting tube 200 without waste. The collector lens group 11 is slightly adjusted in the incident light axis direction so that the light beam guided from the collector lens group 11 to the light projecting tube 200 becomes parallel light.

The parallel rays guided into the light projecting tube 200 are focused on the AS (aperture stop) 27 by the illumination lens 24 after the height of the rays is reduced by the illumination lenses 22 and 23. After passing through the illumination lens 25 and the excitation filter 28, the light is turned back to the objective lens 06 side by the dichroic mirror 29, and the image is formed again at the position of the objective lens pupil 05 of the objective lens 06 and is irradiated on the sample surface. This state is Koehler illumination.

Here, the focusing handle 0 of the microscope body 01
By rotating 2, the sample 09 is moved together with the stage 08 in the observation optical axis direction, and the sample 09 is matched with the focus position of the objective lens 06. The fluorescence emitted from the sample 09 by the illumination light beam emitted from the objective lens 06 is again transmitted to the lens group 07, the dichroic mirror 29, and the absorption filter 30.
Is formed by the imaging lens 41 in the lens barrel 40, and can be observed by the eyepiece 50.

At this time, when an image is arranged at the position of the FS 26, the image is formed on the sample 09 and can be observed by the eyepiece 50. In addition, various sliders described later can be inserted into and removed from the insertion ports 32 to 38 provided in the incident light projection tube 200, so that an optical element and the like can be arranged on the incident illumination optical axis.

Next, the insertion port 3 provided in the light emitting tube 200
The sliders that can be inserted into and removed from the insertion holes 2 to 38 and the insertion ports 32 to 38 will be described with reference to FIGS. The light emitting tube 200 has a cover 51 on the upper side, a rib 55 having a U-shaped notch 52 for passing incident illumination light rays on the inner side, and an insertion hole 53 on the side surface. Screw holes 54 are formed on the upper and lower sides.

A centering member 61 is held between the ribs 55 so as to be sandwiched therebetween. Knob screws 71a, 71b having spherical shapes 72a, 72b at their ends are screwed into the screw holes 54, and abut against the circumference 62 on the side surface of the centering member 61. Thumb screw 71a for each centering member,
Reference numeral 71b is provided at two upper and lower positions, and the respective thumb screws 71a and 71b are arranged at a slight angle.

A V-shaped groove 69 is formed on the side opposite to the side on which the thumb screws 71a and 71b are formed, and the plunger 56 having a spherical tip is fitted therein. The plunger 56 is fitted and held by an adjusting screw 58 screwed and fixed to the incident light projector 200.
A plunger spring 57 is formed inside the plunger 56, and the plunger 56 always presses the centering member 61 in the direction of the thumb screws 71a and 71b.

The centering member 61 has an opening 63 for passing incident illumination rays, and a U-shaped slider insertion portion 67.
The stopper 68 is fixed to the insertion portion 67. A long groove-shaped concave portion 66 is formed on the upper side of the insertion portion 67, and an iterator spring 65 is arranged in the concave portion 66 to apply a force in a direction (a lower side in the figure) of pushing the cylindrical click 64 into the insertion portion 67. The spring 65 is sandwiched by a thrust plate 70 so as not to shift in the direction of the incident light axis. The thrust plate 70 is fixed to the centering member 61 with screws (not shown).

At this time, the rectangular opening (A × B) formed by the U-shaped slider insertion portion 67 of the centering member 61 and the thrust plate 70 serves as an insertion opening for inserting a slider described later. .

The filter slider 171 shown in FIG.
Filter 17 for changing intensity and wavelength characteristics of illumination light beam
2 is fixed by, for example, bonding or the like, and an opening 174 concentric with the filter 172 is formed. V-grooves 173 are formed on both side surfaces of the filter slider 171 at positions where the filter slider 171 abuts on the stopper 68 when the click 64 is pressed when the filter slider 171 is inserted into the slider insertion portion 67 of the centering member 61.

The aperture slider 81 shown in FIG. 7 has an opening 82 for passing incident illumination light rays, and a fitting portion 84 which is a concentric stepped portion. At least four boss holes 85 are provided at the bottom of the fitting portion 84.
Are formed so as to be arranged concentrically with the fitting portion 84.
The boss hole 85 is fitted with one of the crimping pins 86 of a crescent-shaped blade 88 having a thickness of about 0.05 mm and having caulking pins 86 and 87 caulked on different surfaces.

The mating part 90 is rotatably fitted to the fitting part 84. This mawashikan 90 has a slit-shaped notch 91 fitted to the caulking pin 86, and a side opposite to the surface on which the slit upper notch 91 of the mawashikan 90 having an opening 89 for passing incident illumination light rays is formed. There is a step 92 on the surface of
53 is fixed.

The diaphragm slider 81 is formed with a concave portion 95 as shown in the figure. An operating lever 94 can be freely rotated in the concave portion 95 by a rotating shaft 97 fixed to the diaphragm slider 81. , And a pin 153 is fitted in a slit 98 formed in the end face. Further, a thrust cover 99 is fixed to the stop slider 81 by a fixing screw 96 so that the mawasikan 90 and the operation lever 94 do not move in the optical axis direction. V-grooves 93 are formed on both sides of the diaphragm slider at the same positions as the filter slider.

Next, the operation of the configuration described with reference to FIGS. 3 to 7 will be described. First, when inserting the various sliders 171 and 81 into the light emitting tube 200, an opening (A × A) formed by the slider insertion portion 67 in the centering member 61 and the thrust plate 70.
B) is inserted through the insertion hole 53 on the side of the epi-illumination light projection tube 200. At this time, the click 64 is pushed up, and when it is further inserted, it falls into the V-grooves 173 and 93, and the slider 171 and
The position is determined in a state where the end face of 81 is pressed against the stopper.

Next, centering of the slider is performed. In this case,
By turning and loosening the thumb screws 71a, 71b, the centering member 61 is moved in a plane perpendicular to the epi-illumination optical axis by the spherical shapes 72a, 72b at the tips, thereby performing centering. At this time, a force in the direction of pushing back the thumb screws 71a and 71b is applied to the centering member by the plunger spring 57, and the balance is maintained. For example, when the upper thumb screw 71a is pushed in, the centering member 61 moves to the lower left in the figure, and when the lower thumb screw 71b is loosened, it moves to the lower right. At this time, the various sliders 171 and 81 fitted and positioned on the centering member can also move together in a plane perpendicular to the epi-illumination optical axis.

Next, the operation of the stop slider 81 will be described. When the opening / closing lever is moved in the direction of the arrow in the figure, the mawashican rotates, and the slit-shaped notch 9
The caulking pin 87 and the blade 88 fitted to 1 rotate around the caulking pin 86, and as a result, the aperture opens and closes. The maximum and minimum diameters of the aperture are determined by the contact between the wall surface of the recess 95 and the operation lever 94. Finally, the operation of all the above-described configurations will be described.

For example, when a very general microscope is used, the aperture slider 81 is inserted into the insertion ports 38 and 36, and the insertion port 3
A filter slider 171 having a built-in filter for changing the illumination intensity is inserted into 2, 33, 34, 35, and 37 and used.
In this case, the adjustment of the illumination intensity is performed by inserting and removing the filter slider 171, and the operation of the field stop (FS) and the aperture stop (AS) can be performed by the operation lever 94 of the slider 81.
Also, for adjusting the centering of the iris, the thumb screws 71a, 7
1b. If an aperture stop (AS) is not required, the insertion port 36 can also be used by inserting the filter slider 171. In addition, when it is desired to project a pinhole on the specimen surface, a slider having a pinhole fixed is inserted into the insertion port 38 instead of the filter 172 of the filter slider 171 and is placed at the center of the visual field or an arbitrary position of the user. Can be moved. The position adjustment can be performed in the same procedure as that of the diaphragm slider 81.

As described above, according to the first embodiment of the present invention, it is possible to arbitrarily change the combination of the optical elements to be provided in the epi-illumination light pipe, and furthermore, with a simple modification, a pinhole or an arbitrary shape can be seen. It can be projected and adjusted to any position.

According to the first embodiment, since the centering mechanism for adjusting the position of the optical element on the illumination optical axis is provided on the insertion port side, the structure of the slider can be simplified and provided at low cost. In addition, since the shape can be made thin, the insertion opening can be configured in a small space, and the restriction condition for providing the insertion opening can be reduced.

Next, a second embodiment of the present invention will be described with reference to FIGS. 8 and 9 show the slider inserted into the insertion slot, respectively, (a) is a sectional view, (b)
Shows a front view. Since this embodiment is characterized by only the slider, the drawing is also only the slider. Since the light-emitting tube side to be inserted is the same as that of the first embodiment, the same reference numerals are given and the description is omitted.

The auxiliary concave lens slider 101 shown in FIG. 8 is formed with an opening 102 for passing incident illumination light rays and a lens fitting section 105, and a concave lens 104 fits therein.
The ring spring 107 is fixed to the lens fixing V-groove 106 formed on the inner wall of the lens fitting portion 105 with the ring spring 107 dropped. When the auxiliary concave lens slider 101 is inserted into the insertion portion 67 of the centering member 61, a click 64
Is pressed by the auxiliary concave lens slider 101 to stop the stopper 6.
The V-shaped groove 103 is formed at a position where the V-shaped groove 8 abuts.

The auxiliary convex lens slider 111 shown in FIG. 9 is formed with an opening 112 for passing incident illumination light rays and a lens fitting portion 115, and a convex lens 114 is fitted therein.
The ring spring 117 is fixed to the lens fixing V-groove 116 formed on the inner wall of the lens fitting portion 115 with the ring spring 117 dropped.

On both sides of the auxiliary convex lens slider 111,
When inserted into the insertion portion 67 of the centering member 61, click 6
A V-shaped groove 113 is formed at a position where the auxiliary convex lens slider 111 abuts against the stopper 68 by the pressing of 4. The curvatures of the concave lens 104 and the convex lens 114 are determined by the optical system of the epi-illumination device or the like, and may each be composed of a plurality of lenses.
In addition, the concave lens 104 and the convex lens 114 may be fixed by a method such as bonding other than the ring spring fixing.

Next, the operation of the above configuration will be described. First, as in the case where the epi-illumination light tube 200 is combined with an objective lens up-down moving microscope, for example,
A case will be described in which the distance between 0 and the objective lens converter 04 is increased. In this case, since the position of the objective lens pupil 05 is far from the AS 27, even if the collector lens 11 is finely adjusted, the lamp 13 cannot form an image on the objective lens pupil 05, and as a result, Koehler illumination cannot be obtained. At this time, when the auxiliary concave lens slider 101 is inserted into the insertion opening 35 or 37, the image forming position of the lamp 13 goes away.

By adjusting the curvature of the concave lens 104, it becomes possible to form an image of the lamp 13 on the pupil 05 of the objective lens which has moved away, so that Koehler illumination can be obtained.
However, when the auxiliary concave lens slider 101 is inserted into the insertion port 35, the position of the AS 27 also changes. Therefore, it is necessary to insert the aperture slider 81 into the insertion port 37.

In this case, the auxiliary concave lens slider 10
Since the insertion of 1 causes a shift in the epi-illumination optical axis,
It is necessary to adjust the center of the auxiliary concave lens slider 101. In the adjustment method, the image of the lamp 13 is projected on the stage 08 with the objective lens 06 removed, and adjustment is performed so that the projected image of the lamp 13 due to the insertion and removal of the auxiliary concave lens slider 101 does not shift. Even when the auxiliary concave lens slider 101 is inserted into the conventional epi-illumination light pipe, it is possible to eliminate the above-described displacement of the lamp projection image by adjusting the position of the lamp 13, but in this case, the epi-illumination optical axis is inclined. The focus adjustment of the collector lens 11 causes a significant misalignment. Illumination unevenness occurs in actual observation.

Also, for example, the convex lens 114 and the concave lens 1
When the auxiliary convex lens slider 111 and the auxiliary concave lens slider 101 provided with the convex lens 114 and the concave lens 104 so as to form an afocal variable magnification optical system by the combination of the optical lens 04 and the auxiliary concave lens slider 101 are respectively inserted into the insertion openings 32 and 34, the image forming position of the lamp 13 changes. It is possible to change the projection magnification of the lamp 13 onto the objective lens pupil 05 without changing the projection magnification.

The above-mentioned afocal variable power optical system comprises a pair of an auxiliary convex lens slider 111 and an auxiliary concave lens slider 101.
It is not necessary to configure the slider, and it may be configured by three or more types of sliders.

As described above, according to the second embodiment of the present invention, the projection position of the light source can be easily changed, so that the distance between the epi-illumination light pipe and the objective lens can be easily changed. A plurality of different objective mounting positions such as a microscope can be easily applied to a microscope. Further, by changing the projection magnification, it is also possible to easily realize the optimal illumination according to the objective lens used.

Next, a third embodiment of the present invention will be described with reference to FIG. 10, FIG. 11, and FIG. FIG. 10, FIG.
1 and FIG. 12 show a cross section, an upper surface, and a side view of the insertion ports 32-38, respectively. The same parts as those in the first embodiment are denoted by the same reference numerals, and description thereof is omitted. The projection tube 200 has a rib 55 having a U-shaped notch 52 for passing incident illumination light rays, and insertion holes 53a and 53b provided on both side surfaces, and above and below one of the insertion holes 53a. A screw hole 54 is formed. A centering member 131 is held between the ribs 55 in a sandwiched manner.

The screw holes 54 have spherical shapes 72a, 72 at their ends.
The thumb screws 71 a and 71 b having a “b” are screwed in and abut against the circumference 132 on the side surface of the centering member 131. Thumb screws 71a, 71b are spherical shapes 72a, 72b
A hexagonal hole (not shown) is provided on the opposite end surface, and the adjustment knob 151 has a tip 152 that fits into the hexagonal hole. Each thumbscrew 71a, 71b is arranged at a slight angle. For each centering member, the thumb screws 71a and 71b are formed at two upper and lower positions, and the respective thumb screws 71a and 71b are arranged at a slight angle. Thumb screws 71a, 7 of centering member 131
1b is formed on the opposite side to the plane 139
A plunger 121 having a wide flat surface 121 a at its tip is fitted to an adjusting screw 58 fixed to the incident light projector 200 so as to be in contact with the flat surface 139.
A plunger spring 57 is formed inside the plunger 121, and the plunger 56 always presses the centering member 131 in the direction of the thumb screws 71a and 71b. Centering member 1
An opening 133 for passing incident illumination light rays and a U-shaped slider insertion portion 137 are formed in the reference numeral 31.
On the upper side, a long groove-shaped concave portion 66 is formed, in which an ita spring 65 is arranged, and a cylindrical click 64 is inserted into the insertion portion 67.
The force is applied in the direction of pushing in (the lower side in the figure). The spring 65 is provided with a thrust plate 70 so as not to shift in the optical axis direction.
Is sandwiched between. The thrust plate 70 is fixed to the centering member 131 with screws (not shown).

Next, the operation of the above configuration will be described. First, when the various sliders 171, 81, 101, and 111 are inserted into the light emitting tube 200, an opening (A × A) formed by the insertion portion 137 in the centering member 131 and the thrust plate 70.
B) is inserted through the insertion hole 53a or 53b formed on the side surface (can be from either side) of the incident light projection tube 200. At this time, the click 64 is pushed up, and when the click is further inserted, it falls into the V-grooves 173, 83, 103, 113, and the positions of the sliders 171, 81, 101, 111 are determined.

Next, the slider is centered. In this case,
Adjust the tip 152 of the adjustment knob 151 with the thumb screws 71a, 7a.
By screwing or loosening the thumbscrew 71a, 71b while fitting into a hexagonal hole (not shown) of 1b, the centering member 131 is moved in a plane perpendicular to the epi-illumination optical axis by the spherical shapes 72a, 72b at the tips. Moving.

At this time, a force in the direction in which the adjusting screw 58 is pushed back by the plunger spring 57 acts on the centering member 131, and the balance is maintained. For example, when the upper thumbscrew 71a is pushed in, the centering member 131 moves to the lower left in the figure, and when the lower thumbscrew 71b is loosened, it moves to the lower right. At this time, the various sliders 171, 81, and 101 fitted and positioned on the centering member can also move together in a plane perpendicular to the epi-illumination optical axis. Further, in this case, the centering member 131 is connected to the plunger 12.
1 and the centering member 131 is not inclined and the centering member 13
1 will be moved.

According to the third embodiment, the same effects as those of the first embodiment can be obtained. In addition, since the inclination of the slider does not occur due to the centering adjustment, when a stop having a rotating direction such as a rectangular stop is inserted. It is possible to project at the center of the field of view without tilting. In addition, the insertion direction of the various sliders can be performed from either the left or right side, and the centering adjustment knob can be detached when unnecessary, so that the space restricted on the side surface of the apparatus can be reduced.

(Modification) In the present embodiment, only the case where the camera is combined with an upright microscope is described. However, similar effects can be obtained by combining the camera with an inverted microscope.
In addition to the slider described with reference to FIGS. 6 to 9, it is also possible to configure a member that brings some benefit to the user of the microscope by arranging it on the illumination optical axis.

[0063]

According to the present invention, only the necessary optical elements can be easily installed and adjusted, and the optical elements such as auxiliary lenses for changing the illumination optical system can be arranged and adjusted. An illumination device for a microscope can be provided.

[Brief description of the drawings]

FIG. 1 is a plan view of an overall view of a microscope to which the present invention is applied, with an epi-illumination optical system superimposed thereon.

FIG. 2 is a front configuration diagram in which an epi-illumination optical system is superimposed on an overall view of a microscope to which the present invention is applied.

FIG. 3 is a cross-sectional view of the slider insertion port of FIG. 1 for explaining the first embodiment of the microscope illumination device according to the present invention.

FIG. 4 is a top view of the slider insertion port of FIG. 1;

FIG. 5 is a side view of the slider insertion port of FIG. 1;

FIG. 6 is a view for explaining an example of a slider inserted into the insertion slot of FIG. 1;

FIG. 7 is a view for explaining an example of a slider inserted into the insertion slot in FIG. 1;

FIG. 8 is a view for explaining a second embodiment of the illumination device for a microscope according to the present invention, and is a view showing a slider insertion opening portion of FIG. 1;

FIG. 9 is a cross-sectional view of a slider insertion port of FIG. 1 for explaining a second embodiment of the illumination device for a microscope according to the present invention.

FIG. 10 is a cross-sectional view of the insertion port of FIG. 1 for explaining a third embodiment of the illumination device for a microscope according to the present invention.

FIG. 11 is a top view of the insertion port of FIG. 1 for explaining a third embodiment of the illumination device for a microscope according to the present invention.

FIG. 12 is a side view of the insertion slot of FIG. 1 for explaining a third embodiment of the illumination device for a microscope according to the present invention.

[Explanation of symbols]

 01 microscope main body 02 focusing handle 03 stage holder 04 objective lens converter 05 objective lens pupil 06 objective lens 07 lens 08 stage 09 sample 11 collector lens 12 concave mirror 13 lamp 14 mount Reference Signs List 21 LH mount 22-25 Illumination lens 26 FS 27 AS 28 Excitation filter 29 Dichroic mirror 30 Absorption filter 32-38 Insertion port 32, 33 Insertion port 40 Lens tube 41 Imaging lens 42 ... 48 slider 50 ... eyepiece 51 ... cover 53 ... insertion hole 54 ... screw screw 55 ... rib 56 ... plunger 57 ... plunger spring 58 ... adjustment screw 61 ... member 62 ... circumference 63 ... opening 64 ... click 65 ... ita spring 66 ... recess 67 ... slider insertion part 68 ... stopper 69 ... V-groove 0 ... the thrust plate 71a, 71b ... the thumb screw 72a. 72b spherical shape 74 click 81 diaphragm stop 82 opening 84 fitting portion 85 boss hole 86, 87 caulking pin 88 blades 89 opening 90 awashikan 92 step 93 V groove 94 operating lever 95 concave part 96 fixed screw 97 rotary shaft 98 slit 99 thrust cover 100 lamp housing 101 auxiliary concave lens slider 102 opening 103 V groove 104 concave lens 105 lens fitting part 106 V groove 107 Ring spring 111 ... Auxiliary convex lens slider 112 ... Opening 113 ... V groove 114 ... Convex lens 115 ... Lens fitting part 116 ... V groove 117 ... Ring spring 121a ... Flat 121 ... Plunger 131 ... Member 132 ... Circumference 133 ... Opening 137 ... Slider Insertion section 139 Plane 151 Adjustment knob 152 End 153 ... pin 171 ... filter slider 172 ... filter 173 ... V grooves 174 ... opening 200 ... vertical illuminator

Claims (3)

[Claims] 1. A microscope illumination device for irradiating illumination light from a light source onto a sample surface, comprising: a slider holding an optical element inserted into an optical axis of illumination and detachable from an illumination device main body; A plurality of slider insertion ports having the same cross-sectional shape formed in the illumination device main body so as to be aligned in the axial direction; and And a centering member for adjusting the centering in a plane perpendicular to the plane. 2. The illumination device body is a floodlight tube for epi-illumination using Koehler illumination, wherein at least one of a field stop, an aperture stop, and a dimming filter is used as the optical element. The illumination device for a microscope according to claim 1. 3. An optical element according to claim 1, wherein an auxiliary lens for changing a projection position of an objective lens pupil or for changing a projection magnification of a light source is used.
The illumination device for a microscope according to the above.