JP2002066260A - Dialysis container - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a dialysis container in which no air is formed to consequently carry out stable dialysis. SOLUTION: This dialysis container comprises a bottom-closed cylindrical container body opened in one end and a cover having holes for dialysis and installed as to cover a dialysis membrane with the holes and water-tightly engaged with the opening of the container body. The dialysis membrane is so installed as to cover the holes of the cover for dialysis from the outer rim face.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a dialysis container for dialysis of a small amount of a sample in a laboratory or the like.

[0002]

2. Description of the Related Art In the fields of biochemistry and genetic engineering, extraction and purification are performed to analyze proteins, DNA, and the like to be studied. In the extraction purification, a desalting step is required to remove salts remaining in the purification process, and a dialysis method is employed as one of the methods for performing the desalting. In the dialysis operation, as shown in FIG.
The sample solution A is put into the container body 1a, and the lid 1b is attached to the opening thereof via the dialysis membrane 2. Then, the dialysis container 1 is placed in the beaker 3 with the lid 1b facing down. It is immersed in the external solution B, and the dialysis container 1 is fixed to the side surface of the beaker 3 with an adhesive tape 4 or the like. Then, in order to enhance the dialysis effect, the outer dialysate B is stirred by the magnetic stirrer 5 or the like, and the outer dialysate B is appropriately replaced. When the dialysis is completed, the dialysis container 1 is taken out of the dialysis external solution B, and extraneous external water is wiped off. Next, the dialysis container 1 is mounted on a centrifuge with the lid 1b facing upward, and the sample solution A attached to the inner surface of the dialysis container 1 and the dialysis membrane 2 is collected by the centrifugal action. Next, the lid 1b is removed from the container body 1a together with the dialysis membrane 2, and the dialyzed sample solution A in the dialysis container 1 is taken out.

[0003] Such a dialysis container is disclosed in Japanese Unexamined Patent Publication No.
No. 2,998,913. As shown in FIG. 4, the dialysis container includes a bottomed cylindrical container body 6 having an opening 6a through which the sample solution A can be taken in and out, and a lid 7 for opening and closing the opening 6a. When a hole 7a of a predetermined size is provided at a position corresponding to the opening 6a, a dialysis membrane 8 is arranged between the container body 6 and the lid 7, and the lid 7 is attached to the container body 6. The opening 6a and the hole 7a are separated by the dialysis membrane 8.

[0004]

However, in the dialysis container, since the dialysis membrane 8 is adhered to the inner surface of the lid 7, the dialysis container is placed in a container such as the beaker 3 as shown in FIG. When the dialysis membrane 8 is attached with the dialysis membrane 8 facing down and immersed in the external dialysis solution B, as shown in FIG. 4, air C enters the recess 9 defined by the hole 7 a of the lid 7 and the dialysis membrane 8. It may accumulate and the dialysis action may be hindered.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a dialysis container which does not generate air pockets and therefore can perform a dialysis operation stably.

[0006]

A dialysis container according to the present invention has a bottomed cylindrical container body having an opening at one end, a dialysis hole, and is arranged so as to cover the dialysis membrane in the hole. A dialysis container provided with a lid which is watertightly locked at an opening of the container main body, wherein the dialysis membrane is arranged so as to cover a dialysis hole of the lid from an outer end surface.

According to the dialysis container of the present invention, since the dialysis membrane covers the dialysis hole from the outer end surface, the hole does not have a concave portion opened to the outside. Therefore, when the dialysis container is mounted in a beaker or the like by immersing the dialysis membrane in a dialysis external solution with the dialysis membrane facing down, air does not accumulate in the holes, so it is necessary to remove the air. Dialysis with high reliability.

It is preferable that the dialysis membrane has a recess formed on the outer periphery of the outer end of the dialysis opening and is adhered to the recess. In this case, since the dialysis membrane is housed in the concave portion of the lid, the dialysis membrane is more reliably held by the lid.

[0009]

FIG. 1 shows the structure of a dialysis container according to the present invention, and FIG. 2 is a partially enlarged sectional view showing a state where the dialysis container is immersed in an external dialysis solution.

This dialysis container has a bottomed cylindrical container body 10.
And a lid 20 attached so as to cover the opening 11 at one end of the container body 10.

The container body 10 has a flange 12 on one end (upper end) and a male screw 1 on the upper peripheral surface.
Three.

The lid 20 has a cap shape, has a hole 21 having the same diameter as the opening 11 of the container body 10 at the center of the top wall 20a, and has a female screw 22 on the inner peripheral surface of the peripheral wall 20b. are doing. Then, the holes 21 on the outer surface of the top wall 20a
A recess 23 is formed on the periphery of the dialysis membrane 2.
4 is attached. Further, the lid 20 has the top wall 2
An annular rib 25 is formed on the inner surface of Oa at the periphery of the hole 21, and an O-ring 27 is inserted into an annular recess 26 defined by the rib 25, the top wall 20 a, and the peripheral wall 20 b.

Then, the sample solution A is poured into the container body 10, and the female screw 22 of the lid 20 is screwed into the male screw 13 of the container body 10. Then, as shown in FIG.
The ring 27 contacts the upper end of the container body 10 and
0 and the lid 20 are connected in a watertight manner. Then, as shown in FIG. 2, the lid 2 was placed in the tube 30 containing the external dialysis solution B.
0 with the cap 30 attached to the tube 30.
1 is attached.

Thus, the other end (lower end) of the dialysis container accommodated in the tube 30 is in contact with the lid 31 so that the dialysis membrane 18 is securely immersed in the dialysis solution B. Is held. In this state, since the dialysis membrane 18 is substantially flush with the outer surface of the lid 20, the dialysis container is immersed in the dialysis external solution B without accumulation of air bubbles.

Then, the tube 30 is loaded into a vibrator,
By applying vibration to the tube 30, the external dialysate B is stirred. When the dialysis is completed, the cap 31 is removed from the tube 30, and the dialysis container is taken out of the tube 30. Next, the dialysis container is mounted on a centrifuge using the flange 12, and the sample solution A attached to the inner surface of the dialysis container and the dialysis membrane 24 is collected by centrifugal action. 10 and take out the sample solution A inside.

In the above embodiment, the lid 20 is connected to the container body 10 by the screws 22 and 13,
Of course, a part of the lid 20 may be press-fitted into the opening 11 of the container main body 10 or may be fitted to the outer peripheral surface of the upper end of the container main body 10 to couple the lid 20 to the container main body 10.

[0017]

The dialysis container according to the present invention has a bottomed cylindrical container body having an opening at one end and a dialysis hole, which is arranged so as to cover the dialysis membrane in the hole. A dialysis container having a lid that is watertightly locked at the opening of the dialysis container,
The dialysis membrane is arranged so as to cover a dialysis hole of the lid from an outer end surface. According to the dialysis container of the present invention, since the dialysis membrane covers the dialysis hole from the outer end surface, the hole does not have a concave portion opened to the outside. Therefore, when the dialysis container is mounted in a beaker or the like by immersing the dialysis membrane in a dialysis external solution with the dialysis membrane facing down, air does not accumulate in the holes. Highly reliable dialysis can be performed. In addition, a recess is formed in the outer peripheral edge of the dialysis opening in the dialysis container according to the present invention, and the dialysis membrane is attached to the recess, so that the dialysis membrane is housed in the recess of the lid. The dialysis membrane is more securely held on the lid.

[Brief description of the drawings]

FIG. 1 shows one embodiment of a dialysis container according to the present invention, and is a cross-sectional view showing the entire dialysis container.

FIG. 2 is a sectional view of a main part showing a state in which the dialysis container shown in FIG. 1 is immersed in an external dialysis solution.

FIG. 3 is a conceptual diagram showing an installation state of a dialysis container.

FIG. 4 is a cross-sectional view showing the structure of a conventional dialysis container.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 10 Container main body 11 Opening 12 Flange 13 Male screw 20 Lid 20a Top wall 20b Peripheral wall 21 Hole 22 Female screw 23 Depression 24 Dialysis membrane 25 Ring rib 26 Ring recess 27 O-ring 30 Tube 31 Cap A Sample solution B Dialysis external solution

Continued on the front page F term (reference) 3E084 AA02 AA12 AB01 BA02 CA01 CB10 DA01 DB12 FA09 FB01 GA04 GB04 HA04 HB04 HD01 4D006 GA13 JA20A JA22A JA23A JA27A JA70A PC44 4G057 AB08 AB38

Claims (1)

[Claims] 1. A container body having a bottomed cylindrical shape having an opening at one end and a dialysis hole, which is disposed so as to cover the dialysis membrane, and is watertightly locked in the opening of the container body. A dialysis container having a lid, wherein the dialysis membrane is arranged so as to cover a dialysis hole of the lid from an outer end surface.