JP2001349835A - Test paper for determining colored matter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a test paper for determining colored matter capable of obtaining more accurate results of determination in comparison with the conventional test paper. SOLUTION: The test paper 100 for determining colored matter comprises a test paper body 500 having permeability, a reagent 507 for measurement, which exhibits coloration in contact with one or a plurality of samples formed in the same surface of the test paper 500, a water-repellent layer for sealing the test paper body 500 added to the test paper body 500 from the outside to form a permeation path, which makes the samples permeate toward the reagent 507 for measurement, and a joint part 503 for sealing a housing and a sponge 508 for collecting urine, which is a sample collecting and discharging part to collect a predetermined amount of sample and to discharge the collected sample to the permeation path formed by the water-repellent layer.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a test paper for determining the amount of a colored substance contained in a solid phase by measuring transmitted light or reflected light from the solid phase. About.

[0002]

2. Description of the Related Art A measurement method in which various reagents necessary for measurement are impregnated in advance in a solid phase (for example, filter paper), and the target substance can be quantitatively determined only by spotting or impregnating the sample, Recently, it has been widely used as a simple, rapid, and quantitative method.

[0003] The measurement method using the solid phase is often applied to a biological sample, and in this field, it is required to quantify a target colorant in a test sample composed of a complex mixture. There are many. For this reason, it is necessary that the solid phase smoothly generates a color reaction for measuring an object in the solid phase, and that the material has a uniform behavior with respect to light.

However, in the solid phase, the behavior of light and chemical reactions fluctuate subtly. As a result, even if the content of the colored substance is the same, the concentration of the colored substance in the solid phase can be measured by the transmission method or reflected light. It is difficult to obtain reliable results when measuring.

For this reason, much effort has been made to make the material of the solid phase uniform, and it is desired to develop a test paper which can obtain the same measured value even if the state of the solid phase changes slightly.

In the solid phase reagent, filter paper is used as a base material,
Basically, the first reaction reagent and the color reaction reagent are mixed in advance, then impregnated on a filter paper, and dried.

In addition, it has been devised that the sample is easily absorbed when the sample is stably measured, and the liquid phase reaction can be easily performed during the measurement.

Further, it is necessary to avoid reacting with the target component to develop a color before the test of the sample, and when these coexist, they react with each other. is there.

[0009]

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and it is an object of the present invention to provide a test paper for determining the color of a colored substance, which can provide a more accurate quantitative result than conventional test papers. It is intended for.

[0010]

According to the first aspect of the present invention, there is provided a test paper for quantifying a colored substance, comprising a base material having permeability and one or more samples formed on the same surface of the base material. A reagent for measurement to be colored upon contact with the substrate, a permeation path forming unit for sealing the substrate added to the substrate from the outside, and forming a permeation path for permeating the sample toward the reagent for measurement, And a sample collection and discharge unit for discharging the collected sample toward the permeation path formed by the permeation path forming unit.

According to a second aspect of the present invention, in the test paper for quantifying a colored substance according to the first aspect, the measuring reagent includes a pretreatment reagent layer for preventing penetration of impurities in the sample. It is characterized by the following.

According to a third aspect of the present invention, there is provided the test paper for quantitatively determining a colored substance according to the first aspect, wherein the measuring reagent is a pretreatment reagent layer for preventing penetration of impurities in the sample, and an enzyme for the sample. It has an enzyme reaction layer that causes a reaction and a color reaction layer that causes a color reaction by permeation of the sample, and sequentially performs an analysis procedure according to the permeation of the sample from one direction.

According to a fourth aspect of the present invention, in the test paper for quantifying a colored substance according to any one of the first to third aspects, the measuring reagent is provided on the substrate in accordance with a measurement item of the sample. , One or more of which are provided.

According to a fifth aspect of the present invention, in the test paper for quantifying a colored substance according to any one of the first to fourth aspects, the base material includes the type of the test paper and the production conditions of the test paper. Characterized in that an identification unit is provided.

According to a sixth aspect of the present invention, in the test paper for quantitatively determining a colored substance according to any one of the first to fifth aspects, the base material has a direction in which the test paper is mounted on a color inspection apparatus. A substrate state recognition part for recognizing the nature, inclination, and position is provided.

According to a seventh aspect of the present invention, in the test paper for quantifying a colored substance according to any one of the first to sixth aspects, the one or more measurement reagents are jetted on a substrate surface. It is characterized in that it is formed in a trace amount and quantitatively.

[0017]

BEST MODE FOR CARRYING OUT THE INVENTION A test strip for quantitatively determining a colored substance according to an embodiment of the present invention will be described below with reference to the drawings.

First, with reference to FIGS. 1 to 3, the principle of a test paper for quantitatively determining a colored substance according to the present embodiment will be described.

FIG. 1 shows a test paper 1 which is a 360-degree penetrating type sample and has a reagent part 3 at one place. The test paper 1 is formed by applying a reagent part 3 to a base material 2 made of, for example, filter paper or the like, and forming a water-repellent layer on the upper and lower surfaces of the base material 2 as a penetration path forming part made of a film (permeable). 4,5
Is attached so that a sample made of, for example, urine or the like penetrates the reagent section 3 from all directions (360 degrees) of the test paper 1. In FIG. 1, the arrows indicate the permeation path of the sample.

According to the test paper 1, the sample penetrates from all directions or an arbitrary direction. When the sample reaches the center of the test paper 1, the permeation stops naturally and does not permeate any more.

The thickness of the test paper 1 is set to b mm, and a reagent section 3 having a constant area (amm ² ) is applied on the test paper 1 from the entrance of the sample to a target position.
A sample of (a · bmm ³ ) can be collected in the reagent section 3.

Then, when the permeation into the reagent section 3 naturally stops and a certain time elapses, the sample (a · b) in the reagent section 3
mm ³ ) completes the color reaction and is converted into a color product.

FIG. 2 shows a cross section and a plane of the test paper 10 of the sample two-way penetration type.

The test paper 10 is prepared by attaching water-repellent layers 4 and 5 as permeation path forming portions to the upper and lower surfaces of a rectangular base material 2 having one reagent portion 3 to test a sample. The permeation of the paper 10 from a certain direction (both left and right directions in FIG. 2), the permeation into the reagent part 13 naturally stops, and after a certain period of time, the sample in the reagent part 13 finishes the color reaction and is presented. Convert to color.

Also in this case, the thickness of the test paper 10 is set to b mm, and the reagent portion 13 having a constant area (amm ² ) is applied onto the test paper 10 from the entrance of the sample to the target position. By doing so, a sample of (a · bmm ³ ) can be collected in the reagent section 13.

FIG. 3 shows a cross section and a plane of the test paper 20 for sequentially performing a certain analysis procedure.

The test paper 20 allows the reagent applied on the permeation path to react with the sample sequentially by causing the sample to penetrate from the fixed direction of the test paper 20 (both in the left and right directions in FIG. 3). It has a function of automatically executing the procedure on the test paper 20.

That is, the test paper 20 has a rectangular substrate 2 having one reagent portion (color reaction layer) 23 at the center, and symmetrically disposed on both sides of the reagent portion 23 on the substrate 2. And a pair of pretreatment layers 24 coated with a pretreatment reagent and a pair of enzyme reaction layers 25 coated with a reagent for enzyme reaction. A pair of pretreatment layers 24 for removing impurities in the sample
The pair of enzyme reaction layers 25 that cause an enzyme reaction with the sample are formed between the reagent part 23 and the pretreatment layer 24 at both ends of the substrate 2.

A water-repellent layer 4 is provided on the upper and lower surfaces of the substrate 2.
5 is also provided, and a water-repellent layer 26 made of a film (permeable) is provided in a region between the reagents on the substrate 2.

The test paper 20 has a structure in which each of the reagents is separated and applied on the test paper 2 to prevent contamination between the reagents during storage and prevent deterioration of the reagents.

The test paper 20 has a function of sequentially reacting each reagent applied on the permeation path of the sample with the sample and automatically executing a predetermined analysis procedure on the test paper 20. .

The principle of operation of the test paper 20 (A) The test layer (the pretreatment layer 24 and the enzyme reaction layer 25 do not move, or are recognized as not moving due to the penetration of the sample. When an enzyme reaction is used in the test paper 20, the enzyme in the enzyme reaction layer 25 has a higher molecular weight than the sample (substrate), and the permeation (movement) by the solute (water) of the sample is slow. All of the sample (substrate) reaching the color reaction layer 23 passes through the enzyme reaction layer 25 and undergoes an enzyme reaction.

Even when the sample that has undergone the enzyme reaction permeates the reagent section 23, it is recognized that the application position of the reagent section 23 does not move.

(2) Sample Collection Amount (a) When the sample penetrates from two sides of the test paper 20 and reaches the center of the test paper 20, the permeation stops spontaneously and does not permeate any more.

(B) The reagent portion (color reaction layer) 23 is located at the center on the test paper 20 and is located at a target position from the entrance of the sample.
Is applied in a fixed area (amm ² ).

(C) On the two right and left penetration paths of the sample,
The enzyme reaction layer 25 is applied to the target position in a fixed area (the sum of the area of the two right and left areas is bmm ² ).

(D) Assuming that the thickness of the test paper 20 is cmm, when the sample reaches the reagent section 23 and the penetration stops, a sample of (a / b) · cmm ³ can be collected with this test paper 20. .

(F) When the permeation stops and a certain time elapses, the sample ((a / b) · cm
m ³ ) completes the color reaction and is converted to a color product.

(B) When the test layer (pretreatment layer 24, enzyme reaction layer 25) is recognized to move its application position due to the penetration of the sample, (1) In this test paper 20, generally, the pretreatment layer 24 Pretreatment often removes unnecessary and coexisting components in the sample. Therefore, it is desirable that the pretreatment position does not shift the applied position due to the penetration of the sample.

Reagent for enzyme reaction layer 25 (first reaction reagent)
Dissolves in the solute (water) with the permeation of the sample (the solute of the sample: water), and moves in accordance with the permeation of the sample. For this reason, the width of the enzyme reaction layer 25 is smaller than the apparent width applied to the substrate 2 (chromatographic effect of the reagent layer).

As a countermeasure against the chromatographic effect of the reagent layer, the amount of the reagent in the reagent layer is increased (even if the sample has penetrated, the permeation proceeds only for the width of the reagent portion (color reaction layer) 23 for a while). Amount to maintain a saturated solution). Use salts with low solubility of reagents. Increase or decrease the width of the reagent layer.
Etc.

(2) Sample Collection Amount (a) When the sample penetrates from two sides of the test paper 20 and reaches the reagent part 23 at the center of the test paper 20, the permeation stops spontaneously, and further permeates. do not do.

(B) A predetermined area (amm ² ) of a color reaction reagent for the reagent unit 23 is applied to the target position from the entrance of the sample, located at the center on the test paper 20.

(C) On the two permeation paths on the left and right of the sample,
An enzyme reagent for the enzyme reaction layer 25 is applied to a target area in a fixed area (sum of ^two areas on the left and right sides: bmm ² ).

(D) Assuming that the thickness of the test paper 20 is cmm, when the sample reaches the reagent part 23 and the penetration stops, a sample of (a / b) · cmm ³ can be collected with this test paper 20. .

(F) When the permeation stops and a certain period of time elapses, the sample ((a / b) · cm
m ³ ) completes the color reaction and is converted to a color product.

Next, a specific configuration example of the test paper will be described.

There are many types of test papers that can be used in the inspection apparatus, such as a single item test paper, a multi-item test paper, a rate assay test paper, and a multi-item rate assay test paper.

FIG. 4 shows the structure of a single item test paper 445 which is a basic test paper.

The single item test paper 445 is a reagent for quantifying one kind of component (inspection item) contained in the test sample, and various single item test papers 445 are prepared.

As shown in FIG.
Are a measurement test section (reagent section) 451 and a reference test section 452
And the type of the single item test paper 445 and the reagent part 451
And an identification unit 453 composed of a color code in a color that can be easily recognized, which defines the position of the image.

In the measurement test section 451, a test paper main body having a predetermined thickness is impregnated or impregnated with a reagent for quantification, which is colored when the single item test paper 445 reacts with a sample to be quantified. It is formed by attaching filter paper (or a porous polymer).

The reference test section 452 is formed by adhering a test paper main body with a portion not impregnated with a reagent for quantitative determination or a filter paper (or a porous polymer).

The method for impregnating the single-item test paper 445 with the reagent in a small amount and in a fixed amount is a method of jetting from a nozzle by a jet method, and the amount of one pulse to be ejected is unitized. Injection is possible.

The nozzle diameter is as small as about 40 μm, and the amount of ejected reagent is determined by the number of pulses, and the amount is represented by the number of pulses, that is, the frequency. By controlling the number of reagents, it is possible to jet a desired amount of reagent to a fine place in the same manner as when printing on paper by a printing apparatus.

The identification section 453 composed of a color code is provided so that an inspection device (not shown) can recognize additional information indicating the type of reagent, the lot number for quality control, and the like.

In a color code, several kinds of colors can be used for one dot information. Therefore, if ten colors are used for one dot, ten numbers can be used, that is, ten pieces of information can be used for one dot. As a result, in the case of this color code, the display area is smaller than the display area of the normal bar code, and the single item test paper 4
45 can be reduced in size.

Further, the single item test paper 445 of the present embodiment.
The colorimetric state of the reagent and the identification unit 453 can be analyzed as color information by an inspection device using a color CCD.

Next, with reference to FIG.
Will be described.

The multi-item test paper 100 formed using this polymer film functions as a reagent for the purpose of chemical analysis of living urine, and has nine items (of which one item is a reference). Inspection).

In the multi-item test paper 100, nine measurement reagents 507 for measuring nine items are applied in a 3 × 3 arrangement on the surface of a test paper main body 500 made of a permeable base material. In FIG. 5, a total of 12 water-repellent layers 510 are applied to each measurement reagent 507 in a manner sandwiching the upper and lower portions in FIG.
6 is attached. The surface of the test paper main body 500 is covered with a test paper jacket 501 made of a transparent polymer film.

At the center of the upper end of the test paper main body 500 in FIG. 5, a triangular fixing protrusion guide 505 functioning as a part to be recognized as the base material state is formed.

At the lower end of the test paper main body 500, a sponge 508 for urine collection and a polymer film constituting a sample collection and discharge unit by a coating adhesive 504, and an openable and closable cover constituting a sample collection and discharge unit are provided. A bag-shaped urine collection unit jacket 502 with a sealing joint 503 is attached.

When the test paper main body 500 is mounted on an inspection device (not shown), the fixing projection guide 505 serves as a guide at the time of mounting, and prevents the test paper main body 500 from being erroneously mounted in the direction, up and down (front and back). are doing. Further, the position of the test strip main body 500 when measuring the reagent with such a mechanical structure can be accurately positioned.

The Test Paper Jacket (Polymer Film) 501
Protects the test strip body 500, and
This is for ensuring the accuracy of the sample amount of the sample to be brought into contact with the sample.

On the other hand, the urine collecting section jacket 502 is made of a polymer film which is soft and fits to the hand at the time of handling. The purpose of this is to eliminate a dirty image of urine which is a specimen as a general recognition. Sealing joint 5
03 is formed in a bag shape.

At the time of use, the outer sealing joint 50 is used.
3 is opened and the urine collection sponge 508 is opened so as to be open.

The color code 5 as the identification unit
16 is applied on the test paper main body 500. The information indicated by the color code 516 includes the type of the test paper 500,
It is information such as the type of manufacturing.

Although FIG. 5 illustrates the multi-item test paper 100 for testing nine items, other multi-item test papers include measurement items configured in the same manner as the multi-item test paper 100. There are various items such as a multi-item test strip of 4 items (of which one item is for reference) and a multi-item test paper of 11 measurement items (of which 1 item is for reference). 100 is an example.

Next, a method of using the multi-item test paper 100 will be described with reference to FIG.

The first column (leftmost column (1)) in FIG. 6 shows a state in which the jacket sealing joint 503 attached to the urine collection unit jacket 502 attached to the test paper main body 500 is opened.

The second column (2) in FIG.
Is opened, and a sample (for example, urine) is impregnated (sampling) into the sponge 508.

The third column (3) in FIG. 6 shows a state in which the outer sealing joint 503 is closed and sealed.

The fourth column (4) in FIG.
Is pushed, and the sample contained in the sponge 508 is pressed with the air that has entered the urine collection unit jacket 502.
This is a state in which it penetrates to the 0 side.

The fifth column (5) in FIG.
2 is in a state where the sample that has permeated into the test paper main body 500 side penetrates into the measurement reagent 507.

The sixth column (the rightmost column (6)) in FIG. 6 shows that the sample is filled between the test paper main body 500 and the test paper outer cover 501 by pressing the urine collecting section outer cover 502 of the test paper main body 500. It has been done. In this state, each measurement reagent 507 is colored (shown in a black circle in the sixth column of FIG. 6).

Next, the details of the coloring mechanism of the multi-item test paper 100 will be described with reference to FIGS.

As described above, the shape of the test paper main body (base paper) 500 of the multi-item test paper 100 is such that the fixing projection guide 505 suitable for the inspection apparatus is formed. Erroneous mounting such as is prevented.

When a sample (eg, urine) is sucked up by the sponge 508, the sample that has permeated from the sponge 508 passes through the test paper main body 50 along the permeation path shown by the arrow in FIG.
0 penetrates along the upper water-repellent layer 510a, which is a permeation path forming portion provided at 0.

In the case where the enzyme reagent layer 518 provided on the test paper main body 500 as shown in FIG. 9 is provided at the same time, both the enzyme reagent layer 518 and the reagent portion (coloring reagent layer) 506 and the blank portion 520 are provided. The sample permeates through the permeation route as indicated by the arrow.

In the case where the water-repellent layer 530 is also provided between the reagent parts 506 as a permeation path forming part, the water-repellent layer 530 functions as a buffer for allowing the sample to permeate and react with the reagent quantitatively. Let me.

In the above-described embodiment, the penetration path forming portion is formed as a water-repellent layer 26 made of a film (permeable) as shown in FIG. A portion (a portion corresponding to the water-repellent layer 26 in the example shown in FIG. 3) may be cut out (formed as a cavity).

[0083]

According to the first aspect of the present invention, there is provided a test paper for quantification of a colored substance, which can provide accurate quantification results by improving the permeation path of the sample on the base material, the sampling of the sample, and the discharge structure. Can be provided.

According to the second aspect of the present invention, it is possible to provide a test paper for determining the color of a colored substance which can obtain a more accurate quantitative result using a sample from which impurities have been removed.

According to the third aspect of the present invention, an enzymatic reaction
It is possible to provide a test paper for quantification of a colored substance, which can provide an accurate quantification result by a sequential analysis procedure including a color reaction.

According to the fourth aspect of the present invention, it is possible to provide a test paper for quantifying a present color object which can correspond to the number of measurement items of a sample.

According to the fifth aspect of the present invention, since the identification portion including the type of the test paper and the manufacturing conditions of the test paper is provided on the base material, when a large number of test papers are used in the inspection apparatus. This makes it easy to identify individual test papers.

According to the sixth aspect of the present invention, by providing the substrate state recognition portion, it is possible to ensure the accuracy of the arrangement of the test paper when used in the inspection apparatus and obtain an accurate quantitative result. It is possible to provide a test paper for quantification of a colored substance that can be used.

According to the seventh aspect of the present invention, since the reagent is formed in a trace amount and quantitatively on the surface of the base material, a test paper for quantitative determination of the color of a presented substance which can correspond to a trace amount and a quantitative measurement of a sample is provided. Can be provided.

[Brief description of the drawings]

FIG. 1 is an explanatory view showing a test paper of a 360-degree penetration type sample according to an embodiment of the present invention.

FIG. 2 is an explanatory diagram showing a test paper of a sample two-way penetration type test paper according to the embodiment of the present invention.

FIG. 3 is an explanatory diagram showing test papers that sequentially perform a certain analysis procedure in the embodiment of the present invention.

FIG. 4 is a plan view showing a single item test paper according to the embodiment of the present invention.

FIG. 5 is a plan view showing a multi-item test paper according to the embodiment of the present invention.

FIG. 6 is an explanatory diagram illustrating a method of using a multi-item test strip according to the embodiment of the present invention.

FIG. 7 is an explanatory diagram illustrating a coloration mechanism of the multi-item test paper according to the embodiment of the present invention.

FIG. 8 is an explanatory diagram showing a flow of permeation of a sample into a multi-item test paper according to the embodiment of the present invention.

FIG. 9 is an explanatory diagram showing a flow of a sample permeating a multi-item test paper according to the embodiment of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Test paper 2 Substrate 3 Reagent part 4 Water repellent layer 5 Water repellent layer 10 Test paper 13 Reagent part 20 Test paper 23 Reagent part 24 Pretreatment layer 25 Enzyme reaction layer 26 Water repellent layer 100 Multi-item test paper 445 Single item test Paper 451 Reagent part 451 Measurement test part 452 Reference test part 453 Identification part 500 Test paper body 501 Test paper cover 502 Urine collecting part cover 503 Cover sealing joint 504 Cover adhesive 505 Fixing protrusion guide 506 Reagent part 507 Measurement reagent 508 Sponge 510 Water repellent layer 510a Upper water repellent layer 516 Color code 518 Enzyme reagent layer 520 Blank part 530 Water repellent layer

Claims (7)

[Claims] 1. A base material having permeability, a measuring reagent which is colored by contact with one or a plurality of samples formed on the same surface of the base material, and a base material added to the base material And a permeation path forming section for forming a permeation path for permeating a sample toward the measurement reagent, a fixed amount of a sample being collected, and a permeation path formed by the permeation path forming section for the collected sample. A test paper for quantitatively determining a colored substance, comprising: a sample collection / discharge unit that discharges toward the sample. 2. The test paper according to claim 1, wherein the measurement reagent includes a pretreatment reagent layer for preventing penetration of impurities in the sample. 3. The measurement reagent includes a pretreatment reagent layer for preventing penetration of impurities in the sample, an enzyme reaction layer for causing an enzyme reaction with the sample, and a color reaction layer for causing a color reaction by permeation of the sample. 3. The test paper according to claim 1 or 2, wherein an analysis procedure is sequentially performed according to the penetration of the sample from one direction. 4. The method according to claim 1, wherein one or a plurality of the measurement reagents are provided on the substrate according to the measurement items of the sample. Test paper for quantitative determination of color products. 5. The quantitative determination of a colored substance according to claim 1, wherein the base material is provided with an identification section including a type of test paper and a manufacturing condition of the test paper. For test paper. 6. The base material is provided with a base material state recognition part for recognizing a direction, an inclination, and a position when a test paper is mounted on a color inspection apparatus. A test paper according to any one of claims 1 to 5, for quantitatively determining a colored substance. 7. The method according to claim 1, wherein the one or more measuring reagents are formed in a small amount and quantitatively on a substrate surface by a jet injection method. Test paper for quantitative determination of color products.