JP2001323295A - Anti-oxidized fish oil - Google Patents

Abstract

PROBLEM TO BE SOLVED: To impart an anti-oxidizing activity to a fish oil, especially a DHA oil so as to reduce its deterioration. SOLUTION: This anti-oxidized fish oil is obtained by adding 0.1-0.3 wt.% ethoxin, 0.3-0.5 wt.% vitamin E and 0.5-1.0 wt.% lecithin to a purified fish oil.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an antioxidant fish oil, especially D
The present invention relates to an antioxidant fish oil applicable to HA (docosahexaenoic acid) oil.

[0002]

2. Description of the Related Art Conventionally, fish oil has been widely used in various fields. Among such fish oils, DHA oil has recently been used as being good for health. Therefore, conventionally, it has been attempted to incorporate DHA into a chicken egg to absorb DHA into the body as the chicken egg is ingested.
In particular, with the ingestion of chicken eggs, total cholesterol levels and neutral fats have been reduced by the action of DHA.

[0003] However, DHA oil is particularly highly oxidized.
(2) Even after the gas seal is performed, 3.0 meq / kg is changed to 24.8 me after one week.
q / kg. Even if vitamin E and etoxin as antioxidants are added and measured under the same sealing conditions, 8.8 meq / kg after one week has passed.
It has become.

[0004]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a fish oil, particularly a DHA oil, which has an antioxidant effect and has a low deterioration.

[0005]

Means for Solving the Problems In order to achieve the above-mentioned object, the present invention provides a purified fish oil containing 0.1 to 10 etoxin.
It is characterized by adding 0.3% by weight, 0.3-0.5% by weight of vitamin E and 0.5-1.0% by weight of lecithin.

[0006]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS In the following, according to FIG.
The feed was added to the chicken and the eggs of the fed chicken were added to DHA.
The process up to the transition is explained. (Crude oil) First, blending is carried out by heating to obtain an appropriate crude oil. Here, blend refers to DHA in crude oil.
This is performed to keep the content constant.

[0007] For example, the method is carried out so that the same amount of DHA can always be added to a feed by adding a certain amount of crude oil to the feed. DHA in fish oil
The content differs not only for different types of fish but also for the same species of fish due to individual differences. Incidentally, Table 1 shows the results of measuring the DHA content of tuna oil and bonito oil.

[0008]

[Table 1]

Since the DHA contents are different as described above, blending is performed to keep the DHA content constant. Of course, if the DHA content is within a predetermined range without blending, this blending can be omitted. In addition, this blend adjusts the DHA content in the range of 23.2% to 26.3%.

The other active ingredients at this time have the following contents. DHA content 23.3% to 26.3% Etoxin content 0.1% Lecithin content 0.5% Vitamin E content 0.3% (Hot water washing) Next, hot water washing of crude oil is performed.

The hot water washing is performed for the purpose of removing impurities in crude oil. Specifically, using hot and cold water of about 70 ° C., the ratio of crude oil to hot water is set to 90:10, and the temperature is maintained at 65 to 70 ° C. in a centrifuge at 10,000 rpm for 6 seconds. Things. After the completion of such hot water washing, phosphoric acid is added to crude oil by 0.02%.
% By weight. Here, the addition of phosphoric acid is performed as a pretreatment for the next degumming treatment. As the phosphoric acid, phosphoric acid manufactured by Takeda Pharmaceutical Co., Ltd. was used. (Degumming treatment) This degumming treatment is performed to remove gums such as phospholipids from crude oil.

[0012] Specifically, it is carried out in a centrifuge at 10,000 rpm for 6 seconds while maintaining the temperature at 65-70 ° C. After the degumming process is completed,
A prescribed amount of 50% NaOH solution is added to the crude oil. Here, the addition of the 50% NaOH solution is performed as a pretreatment for the next deoxidation treatment. NaO
As the H solution, caustic soda of Sodanikka Co., Ltd. was used. (Deacidification treatment) This deacidification treatment is performed to remove free fatty acids from crude oil.

Specifically, the heat treatment is performed for 6 seconds while maintaining the temperature at 75 to 80 ° C. (Centrifugation) This centrifugation is performed in order to remove the required supernatant oil from the crude oil.

More specifically, it is carried out in a centrifuge at 10,000 to 12000 rpm for 6 seconds while maintaining the temperature at 75 to 80 ° C. (Hot water washing) This hot water washing is performed to remove the alkali remaining in the oil.

Specifically, using hot water of about 80 ° C., the ratio of crude oil to hot water is set to 90:10, and 75 to 8
While maintaining the temperature at 0 ° C., the centrifugation is carried out in a centrifuge at 10,000 to 12000 rpm for 6 seconds. (Dehydration) This dehydration is performed in order to remove a trace amount of water mixed in.

Specifically, a vacuum dryer for maintaining a negative pressure state while heating is performed for 6 seconds. (Decolorization treatment) This decolorization treatment is performed for the purpose of removing pigments and other contaminants in oil.

Specifically, activated clay is added to the oil in a proportion of 2 to 3
% By weight, maintaining the temperature at 110-120 ° C. for 6 seconds, and then passing through a 100-200 mesh filter. As the activated clay, Galleon Earth V1 manufactured by Mizusawa Chemical Industry Co., Ltd. was used. (Filtration) This filtration is performed for completely removing impurities and the like.

Specifically, the check is performed by a check filter system in which a pressure is applied and a filter is passed. The mesh of the filter is 200 mesh. (Addition of antioxidant) This addition of an antioxidant is a key point of the present invention, and is performed to prevent oxidation of oil.

In the case of oil only, the POV value indicating the oxidation state is initially 3.0, but becomes 24.8 in one week. Specifically, it is carried out by adding 0.1% by weight of etoxin, 0.3% by weight of vitamin E, and 0.5% by weight of lecithin to the oil. here,
Etoxin and vitamin E function as radical inhibitors that suppress the chain reaction of autoxidation. Lecithin, on the other hand,
Although it does not have an antioxidant effect by itself, it functions as a synergist (synergist) to increase its effect in the presence of a radical inhibitor.

Table 2 shows the results of measuring the oxidation of each sample under the following conditions. Conditions Container: Glass bottle (N2 gas seal) Temperature: 37 ° C Measurement item: POV (meq / kg) Sample (1) DHA oil (2) DHA oil + Vitamin E (0.3% by weight) (3) DHA oil + Etoxin (0.1% by weight) (4) DHA oil + Vitamin E (0.3% by weight) + Etoxin (0.1% by weight) (5) DHA oil + Vitamin E (0.3% by weight) + lecithin (0.5% by weight) (6) DHA oil + Etoxin (0.1% by weight) + Lecithin (0.5% by weight) (7) DHA oil + Vitamin E (0.2% by weight) + Etoxin (0.1%) (1% by weight) + lecithin (0.3% by weight) (8) DHA oil + Vitamin E (0.3% by weight) + Etoxin (0.1% by weight) + Lecithin (0.5% by weight) (9) DHA Oil + Vitamin E (0.5% by weight) + Etoxin (0.3% by weight) + Cytin (1.0% by weight) (10) DHA oil + Vitamin E (1.0% by weight) + Etoxin (0.5% by weight) + Lecithin (2.0% by weight)

[0021]

[Table 2]

From Table 2, it can be seen that etoxin, vitamin E
The effect of adding lecithin as well as the addition of lecithin was confirmed. Here, it was confirmed that the amount of each additive to be added to the oil was effective in the following range. Etoxin 0.1-0.3% by weight Vitamin E 0.3-0.5% by weight Lecithin 0.5-1.0% by weight Here, if each value is less than or equal to a low value, the effect of addition cannot be expected. On the other hand, when the value is higher than the high value, although there is an effect, an effect commensurate with the increased added amount cannot be expected, so that the additive is wasted.

Here, etoxin is manufactured by Seiko Chemical Co., Ltd.
Etoxin, Vitamin E used was RIKEN E Oil 600 from RIKEN Vitamin Co., Ltd., and Lecithin used was Paste NP manufactured by True Lecithin Industry Co., Ltd. (Product) After adding the antioxidant, it can be shipped as a product. The shipment here includes packing in containers, drums, shipping in tank lorries, and the like. In all cases, nitrogen gas purge is performed to prevent oxidation during storage or transportation. (Blending to feed at feed mill) Here, the contents of the main raw material tank, auxiliary raw material tank and other tanks are weighed, put into a required amount of hopper, and agitated with a main mixer, so that the raw material in the feed is mixed. Is added with DHA.

In addition, as an example of the feed, it was prepared as shown in Table 3 including the mixing ratio shown in% by weight.

[0025]

[Table 3]

Further, the fatty acid composition of such a DHA-containing feed and a general feed to which no animal fat was added was examined. The results are shown in Table 4.

[0027]

[Table 4]

From the results, it can be seen that the contents of myristic acid and arachidic acid were several times increased by using the DHA-containing feed, and that the eicosapentaenoic acid content was 2%.
It can be seen that the docosahexaenoic acid is about 50 times or more, about 0 times. (Transport to the egg farm) The formulated feed is transported to the farm by a bulk truck and stored in the feed tank.

In this case, there are two 15t feed tanks in one poultry house, and one is used up and then the other is used to prevent deterioration of the feed. Despite this use, one tank was used in about 2.5 to 3 days. (Measurement and Feeding) The food is weighed by a feed load cell, and a predetermined amount of food is fed using a known automatic feeder. (Egg collection) The collected eggs were analyzed to check the migration of DHA. Table 5 shows the results.

In this case, after the DHA-containing feed similar to that shown in Table 3 was given to a 250-day-old chicken, the transfer of DHA to the egg at a predetermined week was measured. Also, here, each figure is D per 100 g of edible portion of chicken egg.
It shows the amount of HA.

[0031]

[Table 5]

Further, for the case where the age after the addition is long,
It is shown in "Table 6".

[0033]

[Table 6]

From Tables 5 and 6, it can be seen that when using a feed supplemented with DHA, at least 3 to 4 weeks after the start of addition, DHA can be contained in chicken eggs in a substantially constant amount. Met. Furthermore, it was found that even when the DHA-added feed was added for a long period of time, the amount of DHA in the eggs remained almost constant. In addition, Table 7 shows the same measurement as in Table 5 measured by changing the amount of DHA added to the feed.

[0035]

[Table 7]

From the measurement results, it was found that when the amount of DHA added to the feed was increased, the DHA content in the eggs was also increased. However, the amount added to the feed and the content in the egg are not proportional, and from the above results and experiments including the added amount other than the above, the amount of DHA added in the feed is
It was found that the addition efficiency was the best at about 1.2%.

That is, even when 2.3% is added, which is almost twice as much as 1.2%, the transfer of DHA to chicken eggs is only about 1.4 times. This seems to be because DHA in the DHA-containing feed was excreted.
Therefore, from the viewpoint of economic efficiency, it is considered that about 1.2% is a desirable compounding amount. In addition, the fatty acid content in the yolk of the hen's egg which passed at least 4 weeks after starting to feed the chicken the same compound feed as "Table 3" was measured.
Here, the reason why the measurement was performed on the yolk was that many of the fatty acids were contained in the yolk.

The results are shown in Table 8.

[0039]

[Table 8]

From these results, it was found that the use of the DHA-containing feed increased the DHA content in the egg yolk four times or more. Further, the serum, glucose, total cholesterol, total protein, and neutral fat in the sera of chickens fed the same diet were compared with those of the chickens fed the general diet. The results are shown in "Table 9".

[0041]

[Table 9]

From these results, it was found that the use of the DHA-containing feed reduced the total cholesterol and neutral fat, which was good for chickens. DHA has an odor. Therefore, it was examined whether chickens or eggs were adversely affected by the smell. The results are shown in "Table 10" and "Table 11".

[0043]

[Table 10]

[0044]

[Table 11]

Although there is a slight variation in each, DH
There was no significant change in the egg weight, egg production rate, chicken weight, and daily food consumption between the A-added feed and the general feed. Therefore, because chicken eggs contain DHA, chicken
Even when the feed supplemented with HA was fed, there was no significant external change in the chicken or the laying eggs and the eggs themselves.

[0046]

As described above, the present invention provides a fish oil,
In particular, an antioxidant effect is added to DHA oil to provide a fish oil with less deterioration.

[Brief description of the drawings]

FIG. 1 is a flowchart showing an outline from crude oil to egg collection.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Katsumi Arai 2-264, Kami-Iinuma, Ibaraki-cho, Higashi-Ibaraki-gun, Ibaraki Prefecture Inside the Iinuma Research Laboratory (72) Inventor Minoru Oigawa 1-5, Kandanishikicho, Chiyoda-ku, Tokyo No. F-term in Kentech Tokyo Sales Office (reference) 4H059 BA14 BA33 BA39 BA83 BB05 BB07 BB15 BB18 BB22 BB45 BC06 EA03 EA21

Claims (2)

[Claims] 1. An antioxidant fish oil characterized by adding lecithin to purified fish oil in addition to etoxin and vitamin E. 2. A refined fish oil containing 0.1 to 0.
An antioxidant fish oil comprising 3% by weight, 0.3 to 0.5% by weight of vitamin E and 0.5 to 1.0% by weight of lecithin.