JP2001289862A - Method and kit for detecting protein - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a kit for detecting protein which does not contaminate samples, can safely and quantitatively keep residual reagents, and makes protein detecting time shorter and, in addition, does not allow the colored matter of a reaction reagent to be left in the samples. SOLUTION: In this kit, a detecting medium which removes a protein from the surfaces of the samples and a reagent which reacts to the protein and brings out a color are combinedly housed.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for detecting a protein and a kit for detecting the protein.

[0002]

2. Description of the Related Art Clothes, food, and dwelling, which are the three major elements of material conditions for human life, can be laid off to some extent under environmental and other conditions, but food is indispensable daily. It is.

[0003] Food must therefore always contain adequate nutrients as a source of life support and must not be harmful to health. Although the public health situation in Japan has made remarkable progress and improvement, in terms of food hygiene, it is not completely hygienic from a qualitative point of view.

It is necessary to control the quality and properties of food-related additives, containers, packaging environments, etc. in a hygienic manner so that no hygiene hazards due to eating and drinking occur.

However, in the conventional protein detection method, since the reaction reagent is directly attached to the sample (target), contamination of the sample,
The safety of the residual reagent is a problem, and there is also a problem that a colored substance of the reaction reagent remains in the sample and cannot be easily removed with water or the like.
In addition, the above detection method has poor quantitative properties, and it sometimes takes 3 to 72 hours or more before a detection result is obtained.

[0006]

Therefore, it is an urgent need to solve the above problems, and it is an object of the present invention to provide a sample which is excellent in the contamination of the sample, the safety of the remaining reagent, and has a quantitative property. An object of the present invention is to provide a protein detection method and a protein detection kit in which the detection time of the protein is short without remaining in the sample.

[0007]

The above object of the present invention is achieved by the following constitution.

[0008] 1. A protein detection kit comprising a small container in which a reagent for coloring a protein is enclosed, a detection medium having a detection portion for transferring the protein, and a field in which the reagent and the detection medium come into contact with each other in combination. So,
A kit for detecting a protein, wherein the contact between the reagent and the detection medium is caused by crushing a container in which the reagent is sealed after transferring the protein to the detection medium.

[0009] 2. 2. The protein detection kit according to claim 1, wherein the container enclosing the reagent is made of a flexible member.

[0010] 3. The protein detection kit according to any one of claims 1 to 2, wherein the detection portion is wetted with an aqueous medium before transferring the protein from the sample.

Hereinafter, the present invention will be described in detail.

In the present invention, the protein on the surface of the sample is transferred to a detection medium, and the protein is transferred to a previously prepared protein reaction color reagent solution.
After contacting at 80 to 80 ° C, more preferably 30 to 60 ° C for 1 to 60 minutes, the density of the developed color is determined by, for example, a color sample (for protein quantification) showing a color density corresponding to the protein mass, a calibration curve (protein (Amount-color density), a spectrophotometer, and a color meter, which is a protein detection method capable of quantifying the amount of protein attached to the sample in a short time of 1 to 60 minutes.

The sample used in the present invention is a sample for which it is desired to check whether a protein is attached to the surface.

The detection medium of the present invention is a medium that can transfer proteins on the surface of a sample. The detection medium of the present invention includes, for example, a swab, a membrane filter, a tape with an adsorbent material, a stamp, a strip base, a roller with an adsorbent material, a roller with an adsorbent material, a water-absorbent swab, a filter, a spoid, etc. No. Of these, a detection medium comprising a detection portion and a shaft is preferable, and a shape having a water-absorbing detection portion at the tip of the shaft is more preferable. The sample is preferably made of an elastic member (eg, plastic). Since the surface is pressed when wiping the surface, the efficiency of transferring proteins on the surface of the sample is high, which is preferable.

As a method for transferring the protein on the sample surface to the detection medium, for example, a wiping method, a pressing method, a sucking method, and the like are available. Examples of the wiping method include a method using a swab such as a cotton swab and a method using a membrane filter or the like. As a method of pressing, for example, there is a method of pressing with a tape, a stamp, a strip base, or the like to which an adsorbing material is applied, or a method of pressing with a roller to which an adsorbing material is applied. As a method of sucking, for example, there is a method of sucking with a water-absorbing swab or a filter, or a method of sucking directly with a spoid.

In the case of the sucking method, the effect of the present invention is achieved, for example, by suspending an aqueous solution of the surfactant on the surface of the sample, leaving it for a while, and then floating the protein on the surface to suck the sample.

The detection medium of the present invention preferably has a water-absorbing portion, and the water-absorbing portion is preferably wetted with an aqueous medium in advance.

Examples of the aqueous medium include physiological saline, purified water (eg, distilled water, deionized water, etc.), surfactant aqueous solution, and water-soluble organic solvent (eg, acetone, ethanol, propyl alcohol, methyl ethyl ketone, etc.) aqueous solution. (1 to 95% solution).

Specific examples of the surfactant in the surfactant aqueous solution include the following.

Nonionic activator 1. Sorbitan fatty acid ester 2. Glycerin fatty acid ester 3. Decaglycerin fatty acid ester 4. Polyglycerin fatty acid ester 5. Propylene glycol / pentaerythritol fatty acid ester 6. Polyoxyethylene sorbitan fatty acid ester 7. Polyoxyethylene sorbitan fatty acid ester 8. Polyoxyethylene glycerin fatty acid ester Polyethylene glycol fatty acid ester 10. Polyoxyethylene alkyl ether 11. Polyoxyethylene phytosterol phytostanol 12. Polyoxyethylene polyoxypropylene alkyl ether 13. Polyoxyethylene alkyl phenyl ether 14. Polyoxyethylene castor oil and hydrogenated castor oil 15. Polyoxyethylene lanolin, lanolin alcohol and beeswax derivatives 16. Polyoxyethylene alkylamine. Fatty acid amide 17. 17. Polyoxyethylene alkylphenyl formaldehyde condensate Single chain length polyoxyethylene alkyl ether The above alkyl represents a linear or branched C1-C18.

Anionic surfactant 1. Alkyl sulfates 2. 2. Polyoxyethylene alkyl ether sulfate 3. N-acyl amino acids and salts thereof 4. N-acylmethyltaurine salt 5. polyoxyethylene alkyl ether acetate 6. alkyl sulfocarboxylate, α-olefin sulfonate Alkyl phosphates, polyoxyethylene alkyl ether phosphates The above alkyls represent straight-chain, branched C1-C8.

Cationic surfactant 1. 1. alkyl ammonium salt Alkylbenzylammonium salt The above alkyl represents a straight-chain or branched carbon number of 1 to 8.

Amphoteric surfactant 1. Betaine acetate, imidazolinium betaine 2. Lecithin Commercially available surfactants include, for example, the following.

Nonionic surfactant: Triton X-100 (Wako Pure Chemical Industries, Ltd.), Tween-20 (Wako Pure Chemical Industries, Ltd., Nonidet)
P-40 (Boehringer Mannheim Yamanouchi Co., Ltd.) Anionic surfactant: Triton X-770 (Sigma) Cationic surfactant: Benzalkonium chloride amphoteric surfactant: AM-301 (Nikko Chemicals Co., Ltd.) As the protein reaction coloring reagent, those known in the art can be used, and these reagents can be appropriately prepared and used as a protein reaction coloring reagent solution for detecting the protein of the present invention.

In preparing the protein reaction coloring reagent, sodium borate may be added in an amount of 0.1% to 5% for the purpose of increasing the sensitivity of the amount of the detected protein and shortening the coloring reaction time.

The kit for detecting a protein of the present invention includes, for example,
Detection medium, protein reaction coloring reagent, protein reaction coloring reagent preparation container, container in which the protein is transferred and reacted with the protein reaction coloring reagent solution (eg, a glass or plastic tube with a cap), protein reaction coloring reagent titration For transfer of proteins on the surface of plates, color samples and reagents to the detection medium, refers to a set of frames so that the area to be rubbed is constant.Specifically, it is described in detail in Examples. I do.

[0027]

EXAMPLES The present invention will be described below in detail with reference to examples, but embodiments of the present invention are not limited thereto.

Embodiment 1 Preparation of Reagents Preparation of Protein Reaction Coloring Reagent R Reagents A and B having the following composition were prepared, and reagents A and B were
The mixture obtained at a ratio of 2 is used as a protein reaction coloring reagent R. The absorbance of the coloring solution is measured at a wavelength of 562 nm.

Preparation of Reagent A A deionized aqueous solution having the following composition is used as reagent A. That is, 1% BCA-Na2 (2,2 'bicinchoninic acid disodium), 2% Na2CO3.H2O, 0.16% disodium sulfate,
0.4% NaOH, 0.95% NaHCO3 in deionized water. Also, p
The H is adjusted to pH 11.25 by adding and dissolving a 5% NaOH aqueous solution or NaHCO3.

Preparation of Reagent B A deionized aqueous solution of 4% CuSO4.5H2O is used as reagent B.

The reagents A and B were used in each kit described in detail below.

2. Kit form and measurement procedure The form and measurement procedure of the kit for protein detection are shown below.

During the operation of the kit, care should be taken so that fingers and hands do not directly touch the reagents and the sampling medium during protein sampling.

2-1. Standard type kit Construction of protein detection kit showing one example of the present invention (FIG. 1)
Has the following configuration. That is, in FIG. 1 (a), 5 reagent B (filled in an eye drop bottle-shaped container), 6 saline (filled in an eye drop bottle-shaped container), and a sampling swab 1
(Shape: Swab-shaped, plastic handle 2 mm
φ), 3 glass tubes with cap 2 10φ × 70
mm (1 ml filled with 4 reagents A).

In addition, a thermostat (a block heater or the like whose temperature can be stably set to 60 ° C.), a spectrophotometer (a device capable of measuring the absorbance at a wavelength of 562 nm), a color meter (FIG. 1B), a standard color Scale (Figure 1
(C)) Prepare a test tube stand.

In the case of this kit, a color meter (FIG. 1)
(B)) and a standard color scale (FIG. 1 (c)) are set. 1 (b) and 1 (c), 1a is a standard color scale, 1b is a light, 1c is a colorimetric window, 1d is a standard color scale holder, 1e is a test tube holder,
1f indicates the amount of protein, and 1g indicates the degree of colorimetry.

Kit operation method Open the cap 2 of the glass tube 3 and add 5 reagent B to 2
Add drops and shake gently to mix. Sampling swab 1 contains 2-3 drops of physiological saline 6 (about 100 μl),
Wipe all around a 7 cm square area of the sample. that time,
As a guide, the wiping strength is such that the handle of the sampling swab 1 does not work, and the wiping operation is such that the wiping operation is performed 20 times and the entire target area is wiped, and the sampling swab 1 makes about 40 reciprocations. The sampling swab 1 is immersed in the glass tube 3 as it is. An untreated sampling swab 1 is prepared as a blank, and is similarly immersed in the glass tube 3. Next, each glass tube was placed in a thermostat at 60 ° C and 5 ° C.
Warm for minutes. The glass tube is cooled at room temperature, and the degree of color development is compared with the blank using a color meter. At this time, a standard color scale is prepared in the color meter in advance, and by comparing with the standard color scale, the protein adsorbed on the sampling swab 1 can be semi-quantified.

Further, by measuring the absorbance at a wavelength of 562 nm using a spectrophotometer, the amount of protein adsorbed on the sampling swab 1 (shown in FIG. 7) is accurately measured from a previously determined protein calibration curve. be able to.

2-2. Standard improved kit Construction of kit for protein detection showing another example of the present invention (FIG. 2)
And Preparation This kit has the following configuration. That is, 500 μl of physiological saline 10 is contained in a 10φ × 75 mm glass tube 9, and a flexible case 11 in which 8,12 reagents A and B are sealed is mounted on the lower part of the cap 7. A sampling swab 13 is fixed to the bottom of the cap 7. By gripping both sides of the cap 7, the flexible case 11 is crushed, and the reagents A and B therein fall into the glass tube 9 and come into contact with the sampling swab 13. 14 is a detection part.

In addition, a thermostat (a block heater or the like whose temperature can be stably set to 60 ° C.), a spectrophotometer:
562nm wavelength absorbance can be measured, color meter,
Prepare a standard color scale (if necessary) and test tube stand.

Kit operation method In FIG. 2, the cap 7 is removed, and the sampling swab 13 is removed.
2-1. Sample from the sample as described in the kit procedure. The sampling swab 13 is returned to the glass tube, and the cap 7 is closed. 8, 12 reagents A,
The flexible case 11 containing B is crushed, and the reagents A and B are dropped into the glass tube 9. After the glass tubes are gently shaken to stir the reagents A and B, the glass tubes are heated in a thermostat at 60 ° C. for 5 minutes. The glass tube is cooled at room temperature, and the degree of color development is compared with a blank using a color meter. At this time, a standard color scale is prepared in advance in the color meter, and by comparing with this, it is possible to semi-quantify the protein adsorbed on the sampling swab.

Further, by measuring the absorbance at a wavelength of 562 nm using a spectrophotometer, the amount of protein adsorbed on the sampling swab 13 can be accurately measured from a previously determined protein calibration curve. 14 is a detection part.

2-3. Simplified kit Configuration of a kit for protein detection showing another example of the present invention (FIG. 3)
And Preparation This kit has the following configuration. That is, 15 reagents A, 5
Reagent B (filled in an eye drop bottle-shaped container), reagent R filled container 17 (filled in an eye drop bottle-shaped container), physiological saline 6 (filled in an eye drop bottle-shaped container), sampling swab 1 (shape is 2
-1 same as standard kit), glass tube: 10
It is φ70mm.

In addition, a thermostat (such as a block heater capable of stably setting the temperature to 60 ° C.), a color meter, a standard color scale (if necessary), and a test tube stand are prepared.

Kit operation method 1 ml of 15 reagents A and 2 to 3 drops of 5 reagents B are dropped into the container 17 filled with the reagent R and mixed gently. The reagent R thus prepared is stable at room temperature for 10 days. After dropping 2 to 3 drops of physiological saline 6 on the sampling swab 1 and moistening the sample, the sample is sampled on the sampling swab 1 according to the procedure of the 2-1 kit operation. Next, two to three drops of the reagent R are dropped onto the tip of the sampling swab 1 from the reagent R container 17. The coloring reaction starts immediately at the tip of the sampling swab 1. Therefore, place the tip of the sampling swab 1 on a substrate or the like so that the tip is not stained, and place it in a glass tube at room temperature or, if necessary, at 60 ° C and 1 to 30 ° C.
Warm for minutes. The tip of the sampling swab 1 is colored from very pale green to reddish purple according to the amount of protein adsorbed on the sampling swab 1. If necessary, semi-quantitative analysis is possible by comparing the degree of color development on a standard color scale.

2-4. Plate type kit A kit configuration for protein detection showing another example of the present invention (FIG. 4)
And Preparation This kit has the following configuration. That is, 15 reagents A, 5
Reagent B (filled in an eye drop bottle-shaped container), physiological saline 6
(Filled in an eye drop bottle shaped container), sampling swab 20
(The shape is a cotton ball), a color reaction tray 19, and tweezers 18.

In addition, a thermostat (a heater such as a block heater whose temperature can be stably set to 60 ° C.), a color meter, and a standard color scale (if necessary) are prepared.

Kit operation method 1 ml of 15 reagent A is put into the depression of the color reaction tray 19, and then 2 to 3 drops of 5 reagent B are added. The reagents A and B are mixed by gently shaking the coloring reaction tray 19. The sampling swab 20 (cotton ball) is picked up with tweezers 18, and the sampling swab is made to contain 2-3 drops of physiological saline 6. 2-1 Sample from the sample to the sampling swab as described in the kit operation method. The sampling swab 20 (cotton ball) is dropped into the depression of the color reaction tray 19. The tray is heated at room temperature, if necessary, in a thermostat at 60 ° C. for 1 to 30 minutes.
Cool at room temperature and compare the degree of color development with the blank using a color meter. At this time, a standard color scale is prepared in advance in the color meter, and by comparing with this, it is possible to semi-quantify the protein adsorbed on the sampling swab.

2-5. Strip-type kit Construction of a kit for protein detection showing another example of the present invention (FIG. 5)
And Preparation This kit has the following configuration. That is, FIG.
In (b), 15 reagents A, 5 reagents B (each filled in an eye drop bottle-shaped container), a reagent R filled container 17 (an eye drop bottle-shaped container), and physiological saline 6 (filled in an eye drop bottle-shaped container) ), A sampling filter strip (the shape is a membrane filter with a base), and a color reaction plate.

In addition, a thermostat (a block heater or the like whose temperature can be stably set to 60 ° C.), a color meter, and a standard color scale (as needed) are prepared.

Kit operation method 1 ml of 15 reagents A (filled in the container of eye drop bottle) is filled with 1 ml of 15 reagents A and 5 reagent B in the container 17 filled with reagent R (container of eye drop bottle shape).
(Fill into a container in the form of eye drops), drop 2-3 drops and mix gently. The reagent R thus prepared is stable at room temperature for 10 days. Next, the strip is taken out of the case 24 with the zipper, and the saline 6 is added to the sampling filter 21.
(Fill into a container in the shape of an eye drop bottle) and moisten it by dropping 2-3 drops. Hold the strip base holder 23, and rub the surface of the sampling filter 21 against the sample. The procedure is 2-1. Follow the sampling method shown in the kit operation method. The sample strip base 22 is placed on the color reaction plate, and two to three drops of the reagent R are dropped from the reagent R-filled container 17. Then at room temperature or, if necessary, in a thermostat for 60
Heat the tray at 1 ° C. for 1 to 30 minutes. By preparing a standard color scale in advance and comparing the color development degree with the standard color scale, semi-quantitative determination of the protein adsorbed on the sampling filter 21 is possible.

2-6. Stamp type kit Construction of protein detection kit showing another example of the present invention (FIG. 6)
And preparation This kit is 2-5. Equipment sampling filters 21
Is attached to the stamp holder, others are 2-5
Is the same as

Kit Operation Method In FIGS. 6 (a) and 6 (b), 1 ml of 15 reagents A (filled in an eye drop bottle-shaped container) and 5 B. Drop 2-3 drops of B (filled in a container in the form of eye drops) and mix gently. The reagent R thus prepared is stable at room temperature for 10 days. Next, the stamp holder 25 is taken out, and two or three drops of physiological saline 6 (filled in a container in the form of an eye drop) are dropped on the sampling filter 26 and moistened. Hold the stamp holder 25, and rub the surface of the sampling filter 26 against the sample. The point is 2
-1. Follow the sampling method shown in the kit operation method.
Drop 2-3 drops of reagent R on the filter surface. Thereafter, the holder is heated at room temperature or, if necessary, in a thermostat at 60 ° C. for 1 to 30 minutes. By preparing a standard color scale in advance and comparing the color development degree with the standard color scale, semi-quantitative determination of the protein adsorbed on the filter is also possible.

3. Coloring Form and Sampling Method from Sample We compared the coloring form and sampling method from sample. For examination, 2-1. Standard type, 2-3. A simple measurement kit was used.

As for the form of color development, two conditions of color development in the reagent R solution (2-1. Standard type kit) and color development at the tip of the sampling swab (2-3. Simple type kit), and the sampling method, are as follows. A: Comparative study was conducted under two conditions: A: wetting the medium (appropriate amount of physiological saline is included in the sampling swab), and B: not wetting the medium (using a dry sampling swab). By the combination of these conditions, the degree of color development was compared using a standard color scale in terms of absorbance in the form of color development.

First, 1 ml of a BSA (bovine serum albumin) aqueous solution (100 mg / ml) as a protein was placed in a petri dish, uniformly spread, and dried in a drier. The same treatment was performed on an aqueous solution containing no BSA as a control (blank). These were provided as samples.

In the sampling method, in the method A, 100 μl of physiological saline was contained in the sampling swab, and the protein was wiped from the prepared petri dish according to the kit operation method. In the method B, the same processing as the method A was performed with the sampling swab dried.

The protein detection (color development) of the sampled swab was detected by the following procedure.
In the coloring process 1, the sampling swab sampled by the method A is colored by a procedure according to a standard kit operation method.
The absorbance (λ: 562 nm) of the coloring solution was measured. Coloring process 2
Is the sampling swab sampled by method B,
Color was developed according to the procedure in the simplified kit operation method, and compared with a standard color scale to determine the degree of color development.

The measurement results are shown below.

Examination of Color Form and Sampling Method Sampling method Blank sample Color processing 1 A 0.003 0.375 562 nm O.D * B 0.003 0.155 Color processing 2 A − ++++ Color scale B− + ,
Sampling from a sample requires that the detection medium be appropriately moistened to detect proteins on the surface of the sample.
It turns out that it is better. *: Absorbance at a wavelength of 562 nm.

Further, according to the present invention, there is no contamination of the sample and no protein coloring reaction reagent remains, and it is apparent that the present invention is clearly superior to the conventional method.

4. Investigation of aqueous medium The type of aqueous medium was examined using 1 purified water, 2 physiological saline, 3 surfactant aqueous solution, and 4 ethyl alcohol aqueous solution as the aqueous medium to be contained in the detection medium such as a sampling swab. Sampling of the protein was performed in the same manner as in 3 above, and detection of the protein was performed in the same manner as in the above-described 3 color development processing 1. The results are shown below.

Examination results of various aqueous media (562 nm O.D *) Aqueous media Blank Sample Purified water Distilled water 0.005 0.363 Deionized water 0.004 0.378 Physiological saline 0.005 0.368 Surfactant aqueous solution 0.5% Brij35 0.006 0.415 0.5% Nonidet P-40 0.006 0.425 1.0% Triton X-100 0.007 0.388 1.0% SDS 0.006 0.405 Alcohol 70% ethanol 0.005 0.386 As can be seen from the above, the efficiency of protein transfer from the sample (sample) surface to the detection medium by including an aqueous medium in the detection medium It can be seen that it can be transferred to the target. Above all, it can be seen that the effect is good by including a surfactant aqueous solution. *: Absorbance at a wavelength of 562 nm.

5. Protein calibration curve The protein detection method and the protein detection kit of the present invention are described in 2-1. Shown using a standard kit.

1 ml of each standard BSA aqueous solution (0, 10, 20, 100, 400, 800 μg / ml) is placed in an 8 cmφ petri dish and uniformly spread. Next, it is dried in a drier and used as a sample.

Reagent R in glass tube (10mmφ × 70mm)
1 ml each. For protein sampling, add 0.5% Nonidet P-40 aqueous solution to the sampling swab in the kit.
Including 100 μl, carry out from the previously prepared petri dish according to the operation method of the kit 2-1. Replace sampling swab with reagent R
And heat it in a 60 ° C constant temperature bath for 10 minutes. As a blank, a swab in which no protein is sampled is subjected to the same treatment. After cooling at room temperature, the absorbance of the coloring solution at 562 nm is measured with a spectrophotometer.
For the measurement, double measurement was performed for one sample, and five samples were measured (N = 5). FIG. 7 is a diagram showing a calibration curve.

Table 1 shows the measured values. FIG. 7 shows a protein calibration curve of the protein detection kit of the present invention.

[0068]

[Table 1]

As is clear from Table 1 and FIG. 7, it can be seen that the protein detection method and the protein detection kit of the present invention are excellent in protein quantification.

6. Fig. 4 shows the protein measurement limit of the protein reaction reagent in the protein detection method of the present invention. The protein detection kit is the same as the kit of the present invention.
-1 standard type kit was used.

The reagent R was placed in a glass tube (10 mmφ × 70 mm).
Was added in 1 ml portions. Next, 1 ml of standard BSA aqueous solution (protein concentration: 0, 0.2, 0.3, 0.4, 0.5, 0.8, 1.0 μg / ml) was added, and the mixture was gently shaken. Five of these were prepared and heated in a 60 ° C. constant temperature bath for 10 minutes. After cooling at room temperature, the absorbance (OD) at 562 nm was measured with a spectrophotometer.

Table 2 shows the O.D. at each concentration of the standard BSA aqueous solution.
D value and standard deviation are shown.

FIG. 8 is a diagram showing the measurement limit of the protein reaction reagent used in the protein detection method of the present invention. FIG. 8 shows the area of the average OD value at each measurement point ± 2 SD.

In FIG. 8, □ indicates an average value (n = 10).
The vertical bar indicates the area of the average value ± 2 SD, the vertical axis indicates the absorbance (0.D), and the horizontal axis indicates the protein amount (μg).

[0075]

[Table 2]

The protein measurement limit was 0 μg / standard BSA aqueous solution.
The concentration at which the average value of mlOD.D + the OD value of 2SD and the average value of the OD value at a certain concentration-the OD value of 2SD did not overlap was defined as the lowest measurement limit.

When the measurement limit is measured from this definition, the protein measurement limit in the protein detection method and protein detection kit of the present invention is 0.4 μg.

[0078]

Industrial Applicability The method for detecting a protein and the kit for detecting a protein according to the present invention are excellent in the contamination of a sample, the safety of a residual reagent, and are quantitative, so that a colored substance of a reaction reagent does not remain in the sample. The protein detection time is quick and has a good effect.

[Brief description of the drawings]

FIG. 1 is a block diagram of a protein detection kit (standard type) showing an example of the present invention.

FIG. 2 is a configuration diagram of a protein detection kit (improved type) showing another example of the present invention.

FIG. 3 is a configuration diagram of a protein detection kit (simple type) showing another example of the present invention.

FIG. 4 is a block diagram of a protein detection kit (cotton ball type) showing another example of the present invention.

FIG. 5 is a configuration diagram of a protein detection kit (strip type) showing another example of the present invention.

FIG. 6 is a configuration diagram of a protein detection kit (stamp type) showing another example of the present invention.

FIG. 7 is a diagram showing a protein calibration curve of the protein detection kit of the present invention.

FIG. 8 is a diagram showing the measurement limit of a protein reaction reagent used in the protein detection method of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Sampling swab 2 Cap 3 Glass tube 4 Reagent A 5 Reagent B 6 Physiological saline 7 Cap 8 Reagent A 9 Glass tube 10 Physiological saline 11 Flexible case 12 Reagent B 13 Sampling swab 14 Detection part 15 Reagent A 17 Reagent R Filling container 18 Tweezers 19 Color reaction tray 20 Sampling swab (cotton ball) 21 Sampling filter 22 Strip base 23 Base holder 24 Case with chuck 25 Stamp holder Sampling filter

Claims (3)

[Claims] 1. A small container enclosing a reagent for coloring a protein, a detection medium having a detection portion for transferring the protein, and a container in which the reagent comes into contact with the detection medium are combined and housed in one. A kit for detecting a protein,
A kit for detecting a protein, wherein the contact between the reagent and the detection medium is caused by crushing a container in which the reagent is sealed after transferring the protein to the detection medium. 2. The protein detection kit according to claim 1, wherein the container enclosing the reagent is made of a flexible member. 3. The protein detection kit according to claim 1, wherein the detection portion is wetted with an aqueous medium before transferring the protein from the sample.