JP2001249136A - Method and apparatus for measuring quantity of urinated creatinine and urine component - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a simple method for measuring the quantity of urinated creatinine, a method for measuring urine components by use of the measured quantity of creatinine, and an urine component measuring apparatus, usable for accurately determining, without the need to accumulate urine, the total quantity of discharged urine components and the quantity of urine in a certain period which are used as indices for discovering diseases and for providing guidance to health management. SOLUTION: The method for measuring the quantity of urinated creatinine comprises the process of obtaining, from a weight index value and the quantity of creatinine urinated within a period exceeding one day, a regression equation representing the correlation between the index value and the quantity of urinated creatinine, and the process of measuring the weight index value of a subject and substituting it into the regression equation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for measuring the amount of creatinine excreted in urine and urine components, and an apparatus for measuring urine components.

[0002]

2. Description of the Related Art Measurement of urinary components such as urine sugar, urine protein, urine salt and the like has long been used as an index for disease detection and health care guidance. For example, if urinary sugar is high, there is a possibility of diabetes, if urine protein is high, there is a possibility of nephritis, and if urine salt is high, there is a possibility of high blood pressure, so these symptoms are seen. In such cases, more detailed examinations can be performed, and guidance and treatment for diseases can be performed. Therefore, measurement of urine components is very effective in the medical field.

[0003] In the measurement of urinary components, the amount and concentration of creatinine in urine are usually measured. Creatinine is
Since it is inevitably present in urine as human excrement, it is used for correcting the concentration of urine in measuring other urine components. In addition, urinary creatinine concentration may indicate a possibility of renal disease or uremic disease, and thus urinary creatinine measurement is considered important.

There are various methods for measuring urinary components. For example, as a method for measuring urine salt, which is one of urine components, a method using a test paper for measuring urine salt, A method using a measuring device disclosed in -75727 is performed.

However, since urine is affected by exercise, diet, drinking water, etc. of the subject, the concentration of the urine component differs for each urination. In these methods, the concentration of the urine component in each measurement batch and the urine concentration of the test sample are measured. Although the total amount of components in the medium can be measured, the amount of urinary excretion in one or several days cannot be measured. In addition, since the urine concentration and the urine volume are different for each measurement batch, the measured values are likely to vary, which is insufficient as an index for health care guidance and the like.

For this reason, in order to perform highly accurate measurement by the above-described method, it is necessary to accumulate at least one day's urine (hereinafter referred to as urine collection). In such cases, devices such as urine storage bags are required,
It takes a lot of time and burdens on the subject. In addition, urine must be kept for 24 hours, and there is a problem such as a change in urine components over time.

[0007]

SUMMARY OF THE INVENTION In view of the above, the present invention provides a method of accurately determining the total excretion and urine volume of urine components used as an index for disease detection and health care guidance without storing urine. It is an object of the present invention to provide a simple method for measuring the amount of creatinine excreted in urine, a method for measuring a component in urine using the measured amount of creatinine, and a device for measuring a component in urine, which can be used to obtain a urine.

[0008]

According to the present invention, there is provided a method of obtaining a regression equation showing a correlation between a body weight index value and a urinary excreted creatinine amount in a period exceeding one day, and
Thereafter, the method is a method of measuring the amount of creatinine excreted in urine, which comprises a step of measuring a body weight index value of a subject and substituting the index value into the regression equation. Hereinafter, the present invention will be described in detail.

In the method of measuring the amount of creatinine excreted in urine of the present invention, first, a regression equation showing a correlation between the weight index value and the amount of creatinine excreted in urine in a period exceeding one day is determined. In the method for measuring the amount of creatinine excreted in urine of the present invention, the above-mentioned regression equation greatly affects the measurement accuracy, and the variation (variation) in the measurement value of the amount of creatinine excreted in urine in a certain period used for obtaining the regression equation. ) Is smaller, the accuracy of the regression equation is better. Since the amount of creatinine excreted in urine varies depending on the physical condition and the like, the use of the amount of creatinine excreted in urine within a period of more than one day enhances the measurement accuracy.
In the method for measuring the amount of creatinine excreted in urine of the present invention, the period exceeding 1 day is not particularly limited, but is preferably within 7 days in consideration of the denaturation of the urine sample at the time of measurement. More preferably, the period is within 3 days.

[0010] In the present specification, the "weight index value" means a value representing the weight involved in urinary excretion. The weight index values used in the method for measuring the amount of creatinine excreted in urine of the present invention include body weight, lean body mass, body fat weight, BMI
And the like. Above all, the weight or lean body mass is preferable as the weight index value used in the method for measuring the amount of creatinine excreted in urine of the present invention.

In the present specification, the above-mentioned lean body mass means the body weight obtained by subtracting the body fat weight from the total weight {(body weight) − (body fat weight)}, and the body weight obtained by subtracting the body fat weight and the bone weight from the total weight. {(Body weight)-(body fat weight)-(bone weight)} or the total protein weight of the human body. Above all, in the method for measuring the amount of creatinine excreted in urine of the present invention,
{(Body weight)-(body fat weight)} is preferably used.

In the method for measuring the amount of creatinine excreted in urine of the present invention, the method for measuring the lean body mass is not particularly limited. For example, a method for measuring the body density by an underwater weighing method or the like to calculate the lean body mass. , fat (sebum) indirect methods such as the method of calculating the thickness; DEXA method, a method using a radioactive substance ⁴⁰ K (K ⁴⁰ method), directly lean body mass, such as a method utilizing near infrared (NIR method) And a method of measuring the total amount of protein; a method of measuring and obtaining the impedance of the human body; Above all, a method of measuring and obtaining the human body impedance is preferable.

In the above indirect method, it is necessary to measure the weight of body fat. The body fat weight can be measured using a commercially available body fat meter. When a device capable of measuring the body fat percentage is used, it can be obtained from the body weight and the body fat percentage.

As a device used for measuring lean body mass by measuring the impedance of the human body, any device that can measure body fat weight or lean body mass can be used.
There is no particular limitation on the mounting site of the measurement electrode, the measurement frequency, and the like. Commercially available products can be used as the above device. For example, a weight scale TBF534 with a fat scale (manufactured by Tanita), an OMRON body fat scale HBF301 (manufactured by OMRON), a multi-frequency body fat meter MLT100 (Sekisui Chemical Co., Ltd.) Corporation) and Fat Par Center S-201 (manufactured by Sekisui Chemical Co., Ltd.). Above all, Fat Per Center S-201 is preferable. Fat Par Center S
In the case of -201, a high frequency of 50 kHz is passed through the human body, the impedance value is measured, and the body fat weight, lean body mass, and body water content can be measured.

In the method of measuring the amount of creatinine excreted in urine of the present invention, in addition to the above-mentioned method, a method of obtaining lean body mass using BMI (body mass index) may be adopted. The BMI is a value represented by the following equation (1). BMI = (weight) / (height) ² (1) where the unit of weight is kg and the unit of height is m. That is, a BM of a human having a weight of 60 kg and a height of 1.7 m.
I becomes 21 (kg · m ⁻² ).

When the BMI is used to determine the lean body mass, a regression equation representing the correlation between the BMI and the body fat percentage is first determined, and then the BMI of the subject is measured to determine the regression. It is preferable to obtain the body fat percentage by a body fat percentage measurement method substituted into the equation. The above BM
Since I has a correlation with the body fat percentage, the correlation between the BMI and the body fat percentage is obtained, and a regression equation is obtained.
The body fat percentage can be determined. The regression equation is not particularly limited, and includes, for example, linear regression, quadratic regression, logarithmic regression, and the like. In the method for measuring the amount of creatinine excreted in urine of the present invention, any regression equation may be used as long as the correlation coefficient between the BMI and the body fat percentage is 0.6 or more, preferably 0.8 or more. .

When a linear regression equation is used as the regression equation, the body fat percentage (%) can be expressed by the following equation (3). (Body fat ratio) = a × (BMI) + b (3) In the formula, a is −2 to 2, and b is −10 to 10. For example, in the above equation (3), a = 0.5, b =
In the case of 10, the body fat percentage of a human having a body weight of 60 kg and a height of 1.7 m is 20.5%.

After obtaining the body fat percentage by the above-described method for measuring the body fat percentage, the body fat percentage can be substituted into the following equation (2) to obtain the lean body mass (kg). (Lean body mass) = [{100-(body fat percentage)} / 100] x (body weight) (2) That is, the weight of lean body mass of a person with a body weight of 60 kg and a body fat percentage of 20.5% is 48 kg.

In the method for measuring the amount of creatinine excreted in urine of the present invention, a regression equation showing a correlation between the above body weight index value and the amount of creatinine excreted in urine in a period exceeding one day is determined. The weight index value and the amount of urinary creatinine excreted for a certain period of time exceeding one day are measured and accumulated until now. The regression equation is not particularly limited, and includes, for example, a linear equation, a quadratic equation, and a logarithmic regression. In the method for measuring the amount of creatinine excreted in urine of the present invention, the correlation coefficient between the above-mentioned weight index value and the amount of creatinine excreted in urine in a period exceeding 1 day is 0.6.
As described above, any regression equation, preferably 0.8 or more, may be used.

When a linear regression equation is used as the above regression equation, the relationship between lean body mass and the amount of creatinine excreted in urine in a period exceeding one day is as shown in the following equation (4). The relationship between body weight and the amount of creatinine excreted in urine within a period exceeding one day is represented by the following equation (5). {Amount of urinary creatinine excreted in a period exceeding 1 day (mg)} = c × {fat lean body mass (kg)} + d (4) {Amount of creatinine excreted in urine in a period exceeding 1 day (mg)} = E × {body weight (kg)} + f (5) Assuming that the period exceeding one day in the formula is B day, c is
(5-45) × B, d is (0-5) × B, e is (5-35) × B, and f is (0-5)
× B. For example, in the above equation (4), c = 6, d
In the case of = 3, the amount of creatinine excreted in the urine of a human having a lean body mass of 48 kg for a period exceeding 1 day, for example, 2 days, is 29.
It becomes 1 mg.

When the lean body mass is determined by using the BMI, the relationship between the BMI and the amount of creatinine excreted in urine within a period of more than one day is represented by the following equation (6). {Amount of creatinine excreted in urine in a period exceeding 1 day (mg)} = c × [[100− {a × BMI (kg · m ⁻² ) + b}] / 100] × {body weight (kg)} + d (6) wherein a, b, c, and d are the same as above. The variables a, b, c, d, e, and f in the regression equations (4) to (6) are gender,
After being classified according to factors such as age, it is set as appropriate.

In the method for measuring the amount of creatinine excreted in urine according to the present invention, the amount of creatinine excreted in urine in a period exceeding one day is calculated by measuring the index value of the weight of the subject and substituting it into the regression equation. The subject's weight index value can be appropriately selected according to the weight index value used in the regression equation to be used.

In the method for measuring the amount of creatinine excreted in urine according to the present invention, the amount of creatinine excreted in urine within a period exceeding one day determined by the above regression equation, the amount of creatinine in urine to be examined for one urination and By measuring the urine test volume or the creatinine concentration in the test urine, it is possible to calculate the urine volume for a certain period exceeding one day without storing urine.

For example, if the period exceeding one day is defined as day A, the urine volume on day A is calculated by the following equation (7) or (8). (A-day urine amount) = {(A-day urine excreted creatinine amount) / (test urine creatinine amount)} x (test urine amount) (7) (A-day urine amount) = (A-day urine excreted creatinine amount) ) / (Concentration of creatinine in test urine) (8)

Further, the method for measuring the amount of creatinine excreted in urine of the present invention can be used to measure the total amount and concentration of a substance to be measured in a test urine for one urination, thereby providing an index for disease discovery, health care guidance and the like. It is possible to calculate the total urinary excretion of the other urine component within a period exceeding one day.

Further, by multiplying the value obtained by dividing the amount of creatinine excreted in urine determined by the method for measuring the amount of creatinine excreted in urine of the present invention by the concentration of creatinine in test urine, by the concentration of the substance to be measured in test urine, Urine components can be measured. The present invention 2 relates to a method of measuring the amount of creatinine excreted in urine obtained by the method of measuring creatinine excreted in urine of the present invention 1 to a value obtained by dividing the amount of creatinine excreted in urine by the concentration of creatinine in test urine, and the concentration of the substance to be measured in the test urine. This is a urine component measurement method realized by multiplication.

The urine component is not particularly restricted but includes, for example, urine sugar, urinary salt, urinary albumin, urine protein,
Urinary urobilinogen, urinary bilirubin, urinary N-acetyl-
β-D-glucosaminidase (urinary NAG), urinary β2
Microglobulin, urinary α1 microglobulin, urinary ketone body, urinary polyamine, urinary 5-hydroxyindole acetic acid (urinary 5-HIAA), G
TP, γ-GTP, GOP, amylase, cholesterol, lipid, calcium and the like.

The total urinary excretion of the above-mentioned urinary component on day A can also be calculated from the following equation (9) or (10). (Total A-day urinary excretion of the target component A) = {(Amount of urinary creatinine excreted in the day A) / (total amount of creatinine in the test urine)} x (total amount of the target component in the test urine) (9) Total urinary excretion) = {(Amount of urinary creatinine excreted on day A) / (Concentration of creatinine in urine to be tested)} x (Concentration of target analyte in urine to be tested) (10)

By using the urine component measuring method of the present invention 2, the total amount of urine components and the like can be measured easily and easily, and the burden on the subject can be reduced. The present invention 3 is a urine component measuring device using the urine component measuring method of the present invention 2.

In the method for measuring the amount of creatinine excreted in urine of the present invention, a regression equation showing a correlation is obtained from the body weight index value and the amount of urinary creatinine excreted in a period exceeding one day. By calculating, the amount of creatinine excreted in urine in a period exceeding one day can be easily and simply obtained.

The amount of urinary creatinine obtained by the method for measuring the amount of creatinine excreted in urine of the present invention is the amount of creatinine excreted in urine within a period of more than one day. Even if the concentration is different, it is possible to obtain accurate values of urine volume and excretion of urine components in one day or more by a single urine collection, and there is no need to collect urine.

[0032]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

Example 1 (Measurement by lean body mass method) 10 men in their 20s, 10 women in their 20s, 10 men in their 40s, 10 women in their 40s, 6
Lean body mass was measured for 10 males in their 0s and 10 females in their 60s using a Fat Par Center S-201 (fat measuring device, manufactured by Sekisui Chemical Co., Ltd.). Thereafter, the amount of creatinine excreted in urine for two days was calculated using fx-7200G (Program Calculator, manufactured by Casio) according to the following equation. (Male) 20-39 years old: ｛2 days urinary creatinine excretion (m
g)｝ = 52 × ｛lean body weight (kg)｝ + 3 40-59 years old: 量 2 days urinary creatinine excretion (m
g)｝ = 50 × ｛lean body weight (kg)｝ + 3 60 years old or older: ｛2 day urinary creatinine excretion (mg)｝
= 40 x {lean body mass (kg)} + 4 (female) 20-39 years old: {creatinine excreted in urine on 2 days (m
g)｝ = 50 × ｛lean body weight (kg)｝ + 3 40-59 years old: ｛2 day urinary creatinine excretion (m
g)｝ = 42 × ｛lean body weight (kg)｝ + 3 60 years old or older: ｛2 day urinary creatinine excretion (mg)｝
= 36 × {lean body weight (kg)} + 4 When the correlation between the total amount of creatinine obtained by two-day urine storage for the subject and the amount of creatinine excreted in two-day urine obtained by the lean body mass method is obtained. , R (correlation coefficient) = 0.99, indicating that a good correlation was obtained.

The two-day urine volume was calculated by multiplying the two-day urinary creatinine amount obtained by the above method divided by the test urine creatinine amount at the time of measurement and the urine volume at the time of measurement. The correlation between this value and the value obtained by actually collecting urine for two days is r = 0.
95.

The 2-day urinary glucose was determined by multiplying the creatinine excretion in the 2-day urine determined by the above method by the creatinine concentration in the test urine at the time of measurement and the urinary glucose concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for two days is r
= 0.92.

The two-day amount was determined by multiplying the creatinine excretion in the two-day urine determined by the above method by the creatinine concentration in the test urine at the time of measurement and multiplying by the measured concentration of the substance shown in Table 1. . The correlation between this value and the value obtained by actually collecting urine for two days was determined and is shown in Table 1.

Comparative Example 1 (Measurement by lean body mass method) 10 men in their 20s, 10 women in their 20s, 10 men in their 40s, 10 women in their 40s, 6
Lean body mass was measured for 10 males in their 0s and 10 females in their 60s using a Fat Par Center S-201 (fat measuring device, manufactured by Sekisui Chemical Co., Ltd.). Thereafter, the amount of creatinine excreted in urine per day was calculated using fx-7200G (Program Calculator, manufactured by Casio) according to the following formula. (Male) 20-39 years old: $ daily urinary creatinine (m
g)｝ = 26 × {lean body mass (kg)} + 2 40-59 years old: 量 amount of creatinine excreted in urine per day (m
g)｝ = 25 x ｛lean body mass (kg)｝ +2 60 years old or older: 量 daily urinary creatinine excretion (mg)｝
= 20 x {lean body mass (kg)} + 2 (female) 20-39 years old: {creatinine excreted in urine per day (m
g)｝ = 25 × ｛lean body weight (kg)｝ + 1 40-59 years old: ｛amount of creatinine excreted in urine per day (m
g)｝ = 21 × ｛lean body weight (kg)｝ + 1 60 years old or older: 量 amount of daily creatinine excreted in urine (mg)｝
= 18 × {lean body mass (kg)} + 1 Correlation between the total amount of creatinine obtained by urinating the subject for one day and the amount of daily creatinine excreted in urine obtained by the lean body mass method. , R = 0.94.

The daily urine volume was calculated by multiplying the daily urinary creatinine volume obtained by the above method divided by the test urine creatinine volume at the time of measurement and the measurement urine volume at the time of measurement. The correlation between this value and the value actually collected for one day was r = 0.91.

The daily urinary glucose was determined by multiplying the daily urinary creatinine excretion determined by the above method by the urinary creatinine concentration at the time of measurement and the urinary glucose concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for one day is r
= 0.90.

The daily amount was determined by multiplying the creatinine excreted in urine per day determined by the above method by the creatinine concentration in the test urine at the time of measurement and multiplying by the measured concentration of the substance shown in Table 1. . Table 1 shows the correlation between this value and the value obtained by actually collecting urine for one day.

[0041]

[Table 1]

Example 2 (Measurement by weight method) 10 men in their 20s, 1 woman in their 20s
Weight was measured using a weight scale for each of 0, 10 males in their 40s, 10 females in their 40s, 10 males in their 60s, and 10 females in their 60s. Then, according to the following formula, fx-7200G (program calculator, manufactured by Casio)
Was used to calculate the amount of creatinine excreted in urine on the 3rd day. (Male) 20-39 years old: amount of creatinine excreted in urine on day 3 (m
g) = 66 × {body weight (kg)} + 3 40-59 years old: {3 day urinary creatinine excretion (m
g)｝ = 63 × ｛body weight (kg)｝ + 3 60 years old or older: ｛3 day urinary creatinine excretion (mg)｝
= 51 x {body weight (kg)} + 4 (female) 20-39 years old: {3 day urinary creatinine excretion (m
g) = 54 × {body weight (kg)} + 3 40-59 years old: {3 day urinary creatinine excretion (m
g)｝ = 51 × ｛body weight (kg)｝ + 60 60 years old or older: 量 3 day urinary creatinine excretion (mg)｝
= 42 × {body weight (kg)} + 4 Correlation between the total amount of creatinine obtained by urinating the subject for 3 days and the amount of creatinine excreted in urine on the 3rd day obtained by the body weight method gives r = 0. 89, indicating that a good correlation was obtained.

The 3-day urine volume was calculated by multiplying the 3-day urinary creatinine amount obtained by the above method by the test urine creatinine amount at the time of measurement and multiplying the urine volume at the time of measurement. The correlation between this value and the value obtained by actually collecting urine for 3 days is r = 0.
79.

The three-day urinary glucose was determined by multiplying the creatinine excretion in the three-day urine determined by the above method by the creatinine concentration in the test urine at the time of measurement and the urine sugar concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for 3 days is r =
0.78.

The three-day amount was determined by multiplying the creatinine excretion in the three-day urine determined by the above method by the creatinine concentration in the test urine at the time of measurement and multiplying by the measured concentration of the substance shown in Table 2. . The correlation between this value and the value obtained by actually collecting urine for 3 days was determined and is shown in Table 2.

Comparative Example 2 (Measurement by the weight method) 10 men in their 20s, 1 woman in their 20s
Weight was measured using a weight scale for each of 0, 10 males in their 40s, 10 females in their 40s, 10 males in their 60s, and 10 females in their 60s. Then, according to the following formula, fx-7200G (program calculator, manufactured by Casio)
Was used to calculate the amount of daily creatinine excreted in urine. (Male) 20-39 years old: $ daily urinary creatinine (m
g)｝ = 22 × ｛body weight (kg)｝ + 1 40-59 years old: ｛amount of creatinine excreted in urine per day (m
g)｝ = 21 × ｛body weight (kg)｝ + 1 over 60 years old: ｛amount of creatinine excreted in urine per day (mg)｝
= 17 x {body weight (kg)} + 1 (female) 20-39 years old: {creatinine excreted in urine per day (m
g)｝ = 19 × ｛body weight (kg)｝ + 1 40-59 years old: ｛amount of creatinine excreted in urine per day (m
g)｝ = 17 × ｛body weight (kg)｝ + 1 over 60 years old: ｛amount of creatinine excreted in urine per day (mg)｝
= 14 × {body weight (kg)} + 1 For the above subjects, the correlation between the total amount of creatinine obtained by collecting urine for one day and the amount of creatinine excreted in urine per day obtained by the body weight method is r = 0.80. there were.

The daily urine volume was calculated by multiplying the daily urinary creatinine volume obtained by the above method divided by the test urine creatinine volume at the time of measurement and the urine volume at the time of measurement. The correlation between this value and the value actually collected for one day was r = 0.75.

The daily urinary glucose was determined by multiplying the daily urinary creatinine excretion determined by the above method by the test urine creatinine concentration at the time of measurement and multiplying by the urinary glucose concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for one day is r =
0.73.

The daily amount was determined by multiplying the daily urinary creatinine excretion determined by the above method by the urinary creatinine concentration at the time of measurement and by the measured concentration of the substance shown in Table 2. . Table 2 shows the correlation between this value and the value obtained by actually collecting urine for one day.

[0050]

[Table 2]

Example 3 (Measurement by body fat mass method) 10 men in their 20s, 10 women in their 20s, 10 men in their 40s, 10 women in their 40s, 60
Lean body mass was measured for 10 males in their teens and 10 females in their 60s using an OMRON body fat meter HBF301 (body fat measuring device, manufactured by OMRON). Then, fx-7200G (program calculator,
The amount of creatinine excreted in urine on day 4 was calculated using the product of Casio Corporation. (Male) 20-39 years old: ｛4 days urinary excreted creatinine (m
g) {= 104 x {(body weight)-(body fat mass) (kg)}
+3 40-59 years old: $ 4 urinary creatinine excretion (m
g) {= 100 x {(body weight)-(body fat mass) (kg)}
+3 60 years or older: {Amount of creatinine excreted in urine on day 4 (mg)}
= 80 x {(weight)-(body fat) (kg)} + 4 (female) 20-39 years old: {4 day urinary excreted creatinine (m
g) {= 100 x {(body weight)-(body fat mass) (kg)}
+3 40-59 years old: $ 4 urinary creatinine excretion (m
g)｝ = 84 × ｛(body weight) − (body fat mass) (kg)｝ +
3 60 years and older: {Amount of creatinine excreted in urine on day 4 (mg)}
= 72 × {(body weight) − (body fat mass) (kg)} + 4 Total creatinine value obtained by urinating the subject for 4 days and creatinine excretion in 4-day urine obtained by the body fat mass method And r = 0.93, which indicates that a good correlation can be obtained.

The 4-day urine output was calculated by multiplying the 4-day urinary creatinine excretion determined by the above method by the test urine creatinine amount at the time of measurement, and multiplying the result by the urine amount at the time of measurement. The correlation between this value and the value obtained by actually collecting urine for 4 days is r = 0.
95.

The 4-day urinary glucose was determined by multiplying the 4-day urinary creatinine excretion determined by the above method by the urinary creatinine concentration at the time of measurement at the time of measurement and multiplying by the urinary glucose concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for 4 days is r =
0.95.

The 4-day amount was determined by multiplying the 4-day urinary creatinine amount determined by the above method by the urinary creatinine concentration at the time of measurement and multiplying by the measured concentration of the substance shown in Table 3. . Table 3 shows the correlation between this value and the value obtained by actually collecting urine for 4 days.

Comparative Example 3 (Measurement by body fat mass method) 10 men in their 20s, 10 women in their 20s, 10 men in their 40s, 10 women in their 40s, 60
Lean body mass was measured for 10 males in their teens and 10 females in their 60s using an OMRON body fat meter HBF301 (body fat measuring device, manufactured by OMRON). Then, fx-7200G (program calculator,
The amount of creatinine excreted in urine per day was calculated using CASIO Co., Ltd.). (Male) 20-39 years old: $ daily urinary creatinine (m
g)｝ = 26 × ｛(body weight) − (body fat mass) (kg)｝ +
140-59 years old: daily creatinine excretion in urine (m
g)｝ = 25 × {(body weight) − (body fat mass) (kg)} +
160 years or older: {Amount of creatinine excreted in urine per day (mg)}
= 20 x {(weight)-(body fat) (kg)} + 1 (female) 20-39 years old: {creatinine excreted in urine per day (m
g)｝ = 25 × {(body weight) − (body fat mass) (kg)} +
140-59 years old: daily creatinine excretion in urine (m
g)｝ = 21 × ｛(body weight) − (body fat mass) (kg)｝ +
160 years or older: {Amount of creatinine excreted in urine per day (mg)}
= 18 x {(body weight)-(body fat) (kg)} + 1 The total amount of creatinine obtained by collecting urine for one day and the amount of creatinine excreted in urine daily based on the amount of body fat determined for the subject. Taking a correlation, r = 0.84.

The daily urine output was calculated by multiplying the daily urinary creatinine output obtained by the above method by the test urine creatinine output at the time of measurement and multiplying the result by the urine output at the time of measurement. The correlation between this value and the value actually collected for one day was r = 0.91.

The daily urinary glucose was determined by multiplying the daily urinary creatinine excretion determined by the above method by the urinary creatinine concentration at the time of measurement and the urinary glucose concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for one day is r =
0.90.

The daily amount was determined by multiplying the creatinine excreted in urine per day determined by the above method by the creatinine concentration in the test urine at the time of measurement and multiplying by the measured concentration of the substance shown in Table 3. Table 3 shows the correlation between this value and the value obtained by actually collecting urine for one day.

[0059]

[Table 3]

Example 4 (Measurement by BMI method) 10 men in their 20s, women in their 20s
10, 40s 10 men, 40s 10 women, 60s
About 10 men and 10 women in their 60s,
Body weight was measured using height and weight with a length scale and a weight scale. Profit
BMI = (weight) / (height) ^Two Substitute for
Each BMI was determined. Then, according to the following equation, fx-7
Using 200G (program calculator, manufactured by Casio), 7
The amount of daily creatinine excreted in urine was calculated. (Male) 20-39 years old: creatinine excretion in urine on day 7 (m
g)｝ = 154 × ｛body weight (kg)｝ × [[100- ｛B
MI (kg · m^-2) +2}] / 100] 40-59 years old: {7 days urinary creatinine excretion (m
g)｝ = 147 × {body weight (kg)} × [[100- ｛B
MI (kg · m^-2) +2}] / 100] Age 60 or older: {Amount of creatinine excreted in urine on 7th (mg)}
= 119 x {body weight (kg)} x [[100- $ BMI
(Kg ・ m^-2) +2}] / 100] (female) 20-39 years old: {7-day urinary creatinine excretion (m
g)｝ = 133 × ｛body weight (kg)｝ × [[100- ｛B
MI (kg · m^-2) +2}] / 100] 40-59 years old: {7 days urinary creatinine excretion (m
g)｝ = 139 × {body weight (kg)} × [[100- ｛B
MI (kg · m^-2) +2}] / 100] Age 60 or older: {Amount of creatinine excreted in urine on 7th (mg)}
= 98 × {Body weight (kg)} × [[100- ｛BMI (k
g ・ m^-2) +2｝] / 100] Total creatinine determined by urinating the subject for 7 days
Amount and creatinine excreted in urine on day 7 determined by BMI method
When correlating with the quantity, r = 0.87, and a good correlation is obtained.
It turned out to be obtained.

The 7-day urine volume was calculated by multiplying the 7-day urinary creatinine amount obtained by the above method divided by the test urine creatinine amount at the time of measurement and the urine volume at the time of measurement. The correlation between this value and the value obtained by actually collecting urine for 7 days is r = 0.
77.

The 7-day urine glucose was determined by multiplying the 7-day urinary creatinine excretion determined by the above method by the test urine creatinine concentration at the time of measurement and multiplying by the urinary glucose concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for 7 days is r =
0.76.

The 7-day amount was determined by multiplying the creatinine excretion in the 7-day urine determined by the above method by the creatinine concentration in the test urine at the time of measurement and multiplying by the measured concentration of the substance shown in Table 4. . Table 4 shows the correlation between this value and the value obtained by actually collecting urine for 7 days.

Comparative Example 4 (Measurement by BMI method) 10 men in their 20s, women in their 20s
10, 40s 10 men, 40s 10 women, 60s
About 10 men and 10 women in their 60s,
Body weight was measured using height and weight with a length scale and a weight scale. Profit
BMI = (weight) / (height) ^Two Substitute for
Each BMI was determined. Then, according to the following equation, fx−
Using 7200G (program calculator, manufactured by Casio)
The amount of creatinine excreted in urine per day was calculated. (Male) 20-39 years old: $ daily urinary creatinine (m
g)｝ = 22 × ｛body weight (kg)｝ × [[100- ｛BM
I (kg ・ m^-2) +2}] / 100] 40-59 years old: {Amount of creatinine excreted in urine per day (m
g)｝ = 21 × ｛body weight (kg)｝ × [[100- ｛BM
I (kg ・ m^-2) +2}] / 100] Age 60 or older: {Amount of creatinine excreted in urine per day (mg)}
= 17 × {Body weight (kg)} × [[100- ｛BMI (k
g ・ m^-2) +2}] / 100] (female) 20-39 years old: {creatinine excreted in urine per day (m
g)｝ = 19 × {body weight (kg)} × [[100- ｛BM
I (kg ・ m^-2) +4}] / 100] 40-59 years old: {Amount of creatinine excreted in urine per day (m
g)｝ = 17 × ｛body weight (kg)｝ × [[100- ｛BM
I (kg ・ m^-2) +4}] / 100] 60 years or older: {Amount of creatinine excreted in urine per day (mg)}
= 14 × {body weight (kg)} × [[100- ｛BMI (k
g ・ m^-2) +4｝] / 100] Total creatinine determined by collecting urine for the subject for one day
Amount and daily creatinine excreted in urine determined by BMI method
When correlated with the amount, r = 0.82.

The daily urine volume was calculated by multiplying the daily urinary creatinine volume determined by the above method divided by the test urine creatinine volume at the time of measurement and the urine volume at the time of measurement. The correlation between this value and the value actually collected for one day was r = 0.75.

The daily urinary glucose was determined by multiplying the daily urinary creatinine excretion determined by the above method by the urinary creatinine concentration at the time of measurement at the time of measurement and multiplying by the urinary glucose concentration at the time of measurement.
The correlation between this value and the value obtained by actually collecting urine for one day is r =
0.73.

The daily amount was determined by multiplying the creatinine excreted in urine per day determined by the above method by the creatinine concentration in the test urine at the time of measurement and multiplying by the measured concentration of the substance shown in Table 4. . Table 4 shows the correlation between this value and the value obtained by actually collecting urine for one day.

[0068]

[Table 4]

Tables 1 to 4 show that Examples 1 to 4 using regression formulas using the amount of creatinine excreted in urine in a period exceeding 1 day show Comparative Examples 1 to 4 using the amount of creatinine excreted in urine per day. 4. A higher correlation with the actual measured value was obtained than in 4, and the measurement accuracy was high.

[0070]

The method for measuring the amount of creatinine excreted in urine of the present invention is as described above. Therefore, the amount of creatinine excreted in urine within a certain period can be easily obtained by measuring and calculating the weight index value. it can. Therefore, it is possible to easily obtain an accurate value of the amount of urine excreted as an index of urine volume and disease detection or health care guidance within a certain period of time without collecting urine by a single urine collection.

Claims (7)

[Claims] 1. A step of obtaining a regression equation showing a correlation between a body weight index value and the amount of urinary excreted creatinine within a period of more than one day, and thereafter measuring the body weight index value of the subject and performing the regression. A method for measuring the amount of creatinine excreted in urine, comprising a step of substituting into a formula. 2. The method for measuring the amount of creatinine excreted in urine according to claim 1, wherein the weight index value is body weight or lean body mass. 3. The method for measuring the amount of creatinine excreted in urine according to claim 2, wherein the lean body mass is {(body weight) − (body fat weight)}. 4. In obtaining lean body mass, a regression equation showing the correlation between BMI and body fat percentage represented by the following equation (1): BMI = (weight) / (height) ² (1) And then the subject's BMI
After determining the body fat percentage by measuring the body fat percentage comprising the step of measuring and substituting the regression equation, the body fat percentage,
The method of obtaining lean body mass by substituting into the following formula (2); lean body mass = [{100- (body fat percentage)} / 100] × (body weight) (2) The method for measuring the amount of creatinine excreted in urine according to the above. 5. The lean body mass is determined by measuring body density, near infrared method, D
EXA method, K ⁴⁰ Method according to claim 2 urinary excretion of creatinine amount measuring method according to, characterized in that obtained by using the fat thickness or body impedance value. 6. A urinary excretion creatinine amount determined by the urinary excretion creatinine amount measurement method according to claim 1, 2, 3, or 4, which is divided by a urinary creatinine concentration in the test urine. A urine component measurement method characterized by multiplying the concentration of a target substance. 7. A urine component measuring apparatus using the urine component measuring method according to claim 6.