JP2001137171A - Method for fluorescent display and equipment - Google Patents

Abstract

PROBLEM TO BE SOLVED: To display the shift tendency of a peak wavelength of the fluorescence intensity generated from a measured site irradiated with excitation light as an information source for estimation of tissue properties by observer. SOLUTION: An excitation light L2 from GaN semiconductor laser 114 is irradiated on the biological measurement site 10 and the generated fluorescence L3 is received via an image fiber 103 by a CCD image sensor 125 with an on- chip mosaic filter 123 composed of filters with transmission wavelength band about 480 nm and about 500 nm respectively. A1/A2 computing section 128 calculates the ratio of the light intensity A1 near 480 nm and the light intensity A1 near 500 nm A1/A1 for each image element of the CCD image sensor. The comparison section 131 compares A1/A2 with a reference value RA previously decided based on the fluorescence from a normal tissue and a diseased tissue and displays the compared results deciding that if the A1/A2 is larger, the peak wavelength shifts toward the peak wavelength of fluorescence from the normal tissue and if smaller, it shifts toward the peak wavelength of diseased tissue.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fluorescent display method and apparatus for displaying information corresponding to the characteristics of fluorescent light emitted from a measuring section of a living body irradiated with excitation light.

[0002]

2. Description of the Related Art Conventionally, when a living body is irradiated with excitation light in the excitation wavelength region of an in-vivo dye, a normal tissue and a diseased tissue have different fluorescence intensities, and the living body is observed at a predetermined wavelength. A technique has been proposed in which the localization / infiltration range of a diseased tissue is displayed as a fluorescent image by irradiating excitation light having a wavelength and receiving fluorescence emitted from a dye in the living body.

[0003] When a living tissue is irradiated with excitation light,
The living tissue emits autofluorescence as shown in the spectrum of FIG. It is presumed that the autofluorescence is obtained by superimposing fluorescence from various in-vivo dyes such as FAD, collagen, fibronectin, and porphyrin. FIG. 6 shows representative fluorescence spectra of fluorescence emitted from normal tissue and fluorescence emitted from diseased tissue, measured by the inventors.

As described above, since normal tissue emits strong fluorescence and diseased tissue emits weak fluorescence,
The observer can determine the lesion state based on the light intensity of the displayed fluorescent image. Also, from FIG. 6, in the vicinity of 480 nm, which is the blue band, the fluorescence emitted from the normal tissue has a peak of the spectral intensity, but only a small amount of fluorescence is emitted from the diseased tissue. Understand. That is, it can be said that the wavelength band near 480 nm is a wavelength band in which the difference in the spectral intensity of the fluorescence spectrum emitted from the normal tissue and the spectrum intensity emitted from the diseased tissue appears remarkably. Therefore, the fluorescence emitted from the measuring unit is
A fluorescent display device that cuts out a wavelength band in the vicinity of nm and presents information according to the light intensity in the blue band to an observer has been proposed by the present inventors in Japanese Patent Application No. 11-312942.

Further, in the above-described fluorescent display device, when there is unevenness in a part of a living body, the distance from the excitation light irradiation system to the living body measurement unit is not uniform, and the part of the living body where the excitation light is irradiated is not uniform. The excitation light illuminance may also be non-uniform. The fluorescence intensity is almost proportional to the illuminance of the excitation light, and the illuminance of the excitation light decreases in inverse proportion to the square of the distance. For this reason, a diseased tissue closer to a normal tissue farther from the light source may emit stronger fluorescence, or fluorescence from a normal tissue at a position inclined with respect to the excitation light may be reduced.

In order to eliminate the influence of the difference in the measurement conditions such as the measurement distance and the measurement angle, the spectrum spectra of the fluorescence spectrum emitted from the normal tissue portion and the fluorescence spectrum emitted from the diseased tissue are different. There is known a fluorescent display device that detects the light intensity of the entire measurement wavelength band and the light intensity of a predetermined wavelength band by using the difference, and displays information according to a ratio between the two.

The inventors obtained the intensity ratio for each wavelength when the fluorescence intensity of the entire measurement bandwidth was set to 1 from each fluorescence spectrum, and obtained a distribution diagram of the intensity ratio as shown in FIG. From this figure, it can be seen that the normal tissue has an intensity ratio peak near 480 nm, and the diseased tissue has a peak near 500 nm and 630 nm.
It can be seen that there is an intensity ratio peak near 00 nm. The inventors have found that the difference in the intensity ratio between the fluorescence emitted from the normal tissue and the fluorescence emitted from the diseased tissue increases from the detection result of the peak wavelength of the intensity ratio, around 480 nm,
A fluorescent display device that detects light intensity in a wavelength band near 630 nm or 700 nm, obtains light intensity and ratios in all the measurement wavelength bands, and displays information according to the ratios is also disclosed in Japanese Patent Application No. Hei 11 (1999) -108.
No. 312942 proposes this.

[0008]

SUMMARY OF THE INVENTION However, the inventors have repeatedly conducted experiments for acquiring fluorescence spectra from normal tissues and diseased tissues and analyzed the intensity and waveform of each fluorescence spectrum. It was clarified that there were diseased tissues with high fluorescence intensity and normal tissues with low fluorescence intensity. For this reason, when the measurement site is any of these tissues, information corresponding to the light intensity of the emitted fluorescence or the ratio of the light intensity of the entire measurement wavelength band to the light intensity of the predetermined wavelength band is used for the actual measurement. It turned out that there is a problem that the tissue properties of the site may not be reflected.

The present invention has been made in view of the above problems, and for example, the ratio of the light intensity of the fluorescence emitted from the measurement unit irradiated with the excitation light or the ratio of the light intensity of the entire measurement wavelength band to the light intensity of the predetermined wavelength band is determined by the measurement unit. Even when the tissue properties are not reflected, information according to the shift tendency of the peak wavelength of light intensity, which is information reflecting the tissue properties, can be obtained and displayed so that the observer can infer the tissue properties. It is an object to provide a fluorescent display device with improved reliability.

[0010]

The fluorescent display method according to the present invention is a representative value of the peak wavelength at which the light intensity becomes maximum in the spectral intensity distribution of the fluorescence emitted from the normal tissue of the living body irradiated with the excitation light. Based on a pre-calculated normal peak wavelength or a pre-calculated lesion peak wavelength that is a representative value of the peak wavelength at which the light intensity is maximized in the spectral intensity distribution of the fluorescence emitted from the diseased tissue of the living body irradiated with the excitation light. Determining a shift tendency of a peak wavelength of light intensity in fluorescence emitted from a measurement unit of a living body irradiated with the excitation light, and displaying information according to the shift tendency of the peak wavelength. .

[0011] The fluorescent display device according to the present invention comprises an excitation light irradiating means for irradiating the measurement part of the living body with the excitation light, and a light intensity in a spectrum intensity distribution of fluorescence emitted from normal tissue of the living body irradiated with the excitation light. The representative value of the peak wavelength at which the light intensity is the maximum in the spectrum intensity distribution of the fluorescence emitted from the diseased tissue of the living body irradiated with the excitation light or the normal peak wavelength calculated in advance, which is the representative value of the peak wavelength that is the maximum. Based on a previously calculated lesion peak wavelength, obtain information according to the shift tendency of the peak wavelength of the light intensity in the fluorescence emitted from the measurement unit by the irradiation of the excitation light, and according to the shift tendency of the peak wavelength. And fluorescent display means for displaying the information.

Preferably, the normal peak wavelength is around 480 nm, and the lesion peak wavelength is around 500 nm.

In the first fluorescent display means according to the present invention, in the peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength, the light intensity of the first wavelength band is obtained from the fluorescence emitted from the measuring section. First light intensity detecting means for detecting, second light intensity detecting means for detecting light intensity of a second wavelength band different from the first wavelength band within the peak wavelength band, and a peak of the light intensity Display means for displaying information corresponding to a ratio between the light intensity detected by the first light intensity detection means and the light intensity detected by the second light intensity detection means, as information according to the wavelength shift tendency; It is characterized by having. The first light intensity detecting means and the second light intensity detecting means determine the light intensity of the first wavelength band and the second wavelength from only the wavelength band near the normal peak wavelength or only the wavelength band near the lesion peak wavelength. The light intensity of the band may be detected.

The second fluorescent display means according to the present invention detects the light intensity of the third wavelength band from the fluorescence emitted from the measuring unit in the peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength. A light intensity detecting means for detecting a light intensity of a fourth wavelength band on the longer wavelength side than the third wavelength band within the peak wavelength band;
Light intensity detecting means, and the fourth light intensity detecting means within the peak wavelength band.
Fifth light intensity detecting means for detecting the light intensity of a fifth wavelength band located on the longer wavelength side than the wavelength band of the third light intensity, and the third light intensity as information corresponding to the shift tendency of the peak wavelength of the light intensity. The second derivative of the spectrum intensity distribution obtained from the light intensity detected by the detecting means, the light intensity detected by the fourth light intensity detecting means, and the light intensity detected by the fifth light intensity detecting means. Display means for displaying corresponding information.

The third light intensity detecting means, the fourth light intensity detecting means, and the fifth light intensity detecting means may include only the wavelength band near the normal peak wavelength or only the wavelength band near the lesion peak wavelength. , The light intensity of the first wavelength band, the light intensity of the second wavelength band, and the fluorescence intensity of the third wavelength band may be detected.

Preferably, the peak wavelength band is a wavelength band from 470 nm to 520 nm.

The third fluorescent display means of the present invention is characterized in that the fluorescent light emitted from the measuring section is in a sixth wavelength band which is a shorter wavelength band than a predetermined wavelength near the normal peak wavelength or near the lesion peak wavelength. Sixth light intensity detecting means for detecting light intensity, seventh light intensity detecting means for detecting light intensity in a seventh wavelength band which is a wavelength band longer than the predetermined wavelength, and As the information corresponding to the shift tendency of the peak wavelength, information corresponding to the ratio between the light intensity detected by the sixth light intensity detecting means and the light intensity detected by the seventh light intensity detecting means is displayed. Display means.

The lower limit wavelength of the sixth wavelength band is 420 nm.
The upper limit wavelength of the seventh wavelength band is preferably 550 nm.

The fourth fluorescent display means of the present invention is configured to detect, from the fluorescent light emitted from the measuring section, the light intensity in an eighth wavelength band including the vicinity of the normal peak wavelength or the vicinity of the lesion peak wavelength. Intensity detecting means, ninth light intensity detecting means for detecting light intensity in a ninth wavelength band including the eighth wavelength band, and information corresponding to a shift tendency of the peak wavelength of the light intensity, And display means for displaying information corresponding to the ratio between the light intensity detected by the light intensity detection means and the light intensity detected by the ninth light intensity detection means. is there.

The lower limit wavelength of the ninth wavelength band is 420 nm.
And the upper limit wavelength is preferably 550 nm.

The fifth fluorescent display means of the present invention detects blue light intensity in a predetermined band near a normal peak wavelength as the blue band light intensity from the fluorescent light emitted from the measurement unit by the irradiation of the excitation light. Band light intensity detection means, wherein the display means displays information based on the shift tendency of the peak wavelength of light intensity and the blue band light intensity.

The sixth fluorescence display means of the present invention comprises: total light intensity detection means for detecting light intensity in all measurement wavelength bands from fluorescence emitted from the measurement section by irradiation of the excitation light;
From the fluorescence, a peak light intensity of around 480 nm, 6
A peak light intensity detecting means for detecting light intensity of one wavelength band in a wavelength band near 30 nm or 700 nm, wherein the display means comprises: a shift tendency of the peak wavelength of the light intensity; And displaying information based on the ratio of light intensities in the entire measurement wavelength band.

[0023] The seventh fluorescent display means of the present invention comprises a blue band detecting means for detecting, as the blue band light intensity, the light intensity of a predetermined band near a normal peak wavelength from the fluorescence emitted from the measuring unit by the irradiation of the excitation light. From the fluorescence intensity, from the fluorescence, the total light intensity detection means for detecting the light intensity in the entire measurement wavelength band, and from the fluorescence, as a peak light intensity, in a wavelength band near 480 nm, 630 nm or 700 nm. A peak light intensity detecting means for detecting the light intensity of one wavelength band, wherein the display means includes a shift tendency of the peak wavelength of the light intensity, the blue band light intensity, the peak light intensity, and the total measurement. It is characterized by displaying information based on the ratio of the light intensity in the wavelength band.

In the fluorescent display device of the present invention, it is desirable to use, as the excitation light, light having a wavelength of 380 nm to 420 nm, which deviates from the characteristic light intensity peak of normal tissue. Further, the excitation light irradiation means is GaN
Based semiconductor lasers are preferred.

It should be noted that the above-described fluorescent display device may detect a fluorescent image two-dimensionally, or may detect one or more biological parts.
A device that detects the fluorescence intensity for each point is also applicable.

The display means may use any display method. For example, the light intensity in a predetermined wavelength band including 480 nm may be changed to 630 nm or 700 nm.
A method of detecting the light intensity in a predetermined wavelength band including, and displaying the ratio of the two on a monitor or a printer or the like, or simply changing the luminance value or the color tone of the display color according to the ratio of the light intensity. The method may be used, and the type does not matter.

The vicinity of the predetermined wavelength according to the present invention is a wavelength range including the predetermined wavelength and the wavelength band around the predetermined wavelength, and the ratio of the light intensity includes the ratio and the difference.

[0028]

According to the present inventors, in the distribution diagram of the intensity ratio from the fluorescence collected from the normal tissue and the diseased tissue, as shown in FIG. 7, the intensity ratio of the fluorescence emitted from the normal tissue becomes maximum at around 480 nm. , That the intensity ratio of the fluorescence emitted from the diseased tissue also has a peak, and near 480 nm,
Focusing on the fact that the fluorescence emitted from the normal tissue and the fluorescence emitted from the diseased tissue have only one peak, the fluorescence emitted from the normal tissue and various diseased tissues is
The peak wavelength of light intensity existing in the wavelength band of 420 nm to 550 nm was detected. FIG. 8 shows the detection results.

From this figure, it can be seen that although the peak wavelength of the fluorescence detected from the normal tissue varies slightly, it is almost 480.
It can be seen that the peak wavelength of the fluorescence detected from the diseased tissue is concentrated in the vicinity of nm, and is substantially distributed in a wavelength band of 490 nm to 510 nm centered at 500 nm. In addition, comparing the peak wavelengths of normal tissue with a pre-cancerous fibroma tissue and a linear cancer tissue that has progressed to cancer, the peak wavelength shifts from 480 nm to longer wavelengths as the normal tissue progresses to cancer. It can be confirmed that it changes to around 510 nm.

Thus, in the fluorescence spectrum of the fluorescence emitted from the living body measurement section irradiated with the excitation light, 4
It has been clarified that the shift tendency of the peak wavelength of the light intensity existing in the wavelength band of 20 nm to 550 nm can be regarded as reflecting the tissue properties of the biometric part.

That is, the shift tendency of the peak wavelength of the light intensity existing in the wavelength band of 420 nm to 550 nm is obtained from the fluorescence detected from the measurement part whose tissue property is unknown, and information corresponding to the shift tendency is displayed. Thus, the observer can estimate whether the measurement site is a normal tissue or a diseased tissue.

From the above examination results, according to the fluorescent display device of the present invention, the normal peak wavelength, which is a representative value of the peak wavelength at which the light intensity of the fluorescent light emitted from the normal tissue is maximized, or the fluorescent light emitted from the diseased tissue. Determine in advance the lesion peak wavelength, which is a representative value of the peak wavelength at which the light intensity is maximum,
On the basis of these, the shift tendency of the peak wavelength of the light intensity in the fluorescence emitted from the measuring unit is obtained, and the apparatus is provided with a fluorescence display unit that displays information according to the shift tendency of the peak wavelength, whereby the excitation light is irradiated. Even if the light intensity of the fluorescent light emitted from the measurement unit or the ratio of the light intensity of the entire measurement wavelength band to the light intensity of the predetermined wavelength band does not reflect the tissue property of the measurement unit, the information reflecting the tissue property is It can be displayed, and the reliability of the display information can be improved.

From the analysis results of the fluorescence spectra by the inventors, it is clear that reliable information can be obtained by setting the normal peak wavelength to 480 nm and the lesion peak wavelength to 500 nm. Have been.

In the fluorescent display device provided with the first fluorescent display means of the present invention, the first wavelength existing in the peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength is obtained from the fluorescence emitted from the measuring section. Determining the light intensity of the band and the light intensity of a second wavelength band different from the first wavelength band;
Information corresponding to the ratio between the two is displayed. For example, if 470 nm to 520 nm is set as the peak wavelength band, there is only one peak of light intensity within this wavelength band regardless of whether the biometric unit is a normal tissue or a diseased tissue. Therefore, the ratio of the light intensity of the two wavelengths becomes a value reflecting the tendency of the shift of the peak wavelength of the light intensity, and the reliability of the displayed information can be improved.

Also, based on only the wavelength band near the normal peak wavelength or only the wavelength band near the lesion peak wavelength, the light intensity of the first wavelength band and the light of the second wavelength band different from the first wavelength band are determined. By obtaining the intensity and displaying the information according to the ratio of the two, it is possible to display the information according to the shift tendency including the relative shift amount from the vicinity of the normal peak wavelength or the vicinity of the lesion peak wavelength, further improving the reliability of information. Can be improved.

In the fluorescent display device having the second fluorescent display means of the present invention, the third wavelength existing in the peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength is obtained from the fluorescence emitted from the measuring section. The light intensity of the band, the light intensity of the fourth wavelength band on the longer wavelength side than the third wavelength band, and the light intensity of the fifth wavelength band on the longer wavelength side than the fourth wavelength band are obtained, and the spectrum intensity distribution is obtained. Are displayed as information reflecting the shift tendency of the peak wavelength of the light intensity. Since information is extracted from the three wavelength bands, the reliability of the information is improved.

Further, from only the vicinity of the normal peak wavelength or only the vicinity of the lesion peak wavelength, the light intensity of the third wavelength band, the light intensity of the fourth wavelength band, and the light intensity of the fifth wavelength band correspond to the second derivative. If a value to be obtained is obtained and information corresponding to the value is displayed, the shift tendency of the peak wavelength with reference to the vicinity of the normal peak wavelength or the vicinity of the lesion peak wavelength can be displayed.

[0038] In the fluorescent display device provided with the third fluorescent display means of the present invention, the fluorescence emitted from the measuring section is shorter than a predetermined wavelength existing in the peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength. The light intensity in the sixth wavelength band, which is the wavelength band on the wavelength side, and the light intensity in the seventh wavelength band, which is the wavelength band longer than the predetermined wavelength, are obtained, and information according to the ratio between the two is obtained as the peak wavelength. Is displayed as a display according to the shift tendency. For example, if the lower limit wavelength of the sixth wavelength band is 420 nm and the upper limit wavelength of the seventh wavelength band is 550 nm, both the light intensity on the short wavelength side and the light intensity on the long wavelength side from the predetermined wavelength become sufficiently large values. Therefore, the reliability of information is further improved.

In the fluorescent display device having the fourth fluorescent display means of the present invention, the normal peak wavelength existing in the peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength is obtained from the fluorescence emitted from the measuring section. Light intensity in an eighth wavelength band including the vicinity or near the lesion peak wavelength;
The light intensity in the ninth wavelength band including the eighth wavelength band is obtained, and information according to the ratio between the two is displayed as a display according to the shift tendency of the peak wavelength. For example, if the lower limit wavelength of the ninth wavelength band is 420 nm and the upper limit wavelength is 550 nm,
Since the shift tendency of the peak wavelength based on the vicinity of the normal peak wavelength or the vicinity of the lesion peak wavelength can be displayed, and the light intensity in the ninth wavelength band becomes a sufficiently large value, the reliability of the information is also improved.

In the fluorescent display device provided with the fifth fluorescent display means of the present invention, the information based on the shift tendency of the peak wavelength of the fluorescent light intensity and the blue band light intensity is displayed, so that the measured fluorescence spectral intensity is displayed. Information that takes into account the characteristics of both the peak and the shift tendency of the peak wavelength can be displayed, and the reliability of the information is improved.

In the fluorescent display device having the fluorescent display device according to the sixth aspect of the present invention, the shift tendency of the peak wavelength of the fluorescent light intensity, the light intensity in the blue band, and the light intensity in the entire measurement wavelength band are equal to four.
By displaying information based on the ratio of the peak light intensity, which is the light intensity of one wavelength band in the wavelength band near 80 nm, 630 nm, or 700 nm, the shift of the measured peak wavelength of the fluorescence. Information that takes into account both the tendency and the characteristics of the spectrum waveform can be displayed, and the reliability of the information is improved.

In the fluorescent display device having the fluorescent display means of the seventh aspect of the present invention, the shift tendency of the peak wavelength of the fluorescent light intensity, the blue band light intensity, and the light intensity of the entire measurement wavelength band are 48.
By displaying information based on the ratio of the peak light intensity, which is the light intensity of one wavelength band in the wavelength band near 0 nm, 630 nm, or 700 nm, the shift tendency of the measured peak wavelength of the fluorescence can be improved. , Spectral intensity and
Information that takes into account the three characteristics of the spectrum waveform can be displayed, and the reliability of the information is further improved.

In the fluorescence emitted from the normal tissue, the intensity deviates from the vicinity of 480 nm where the light intensity is characteristically increased.
By using the excitation light having a wavelength of 80 nm to 420 nm, fluorescence having a fluorescence spectrum having a desirable waveform is emitted, and the reliability of displayed information is improved. In addition, by using a GaN-based semiconductor laser as the excitation light irradiation means, it is possible to reduce the size and cost of the device.

[0044]

Embodiments of the present invention will be described below in detail with reference to the drawings. First, an endoscope apparatus according to a first specific embodiment to which a fluorescent display device according to the present invention is applied will be described with reference to FIGS.
FIG. 1 is a schematic configuration diagram of an endoscope apparatus to which the fluorescent display device according to the present invention is applied, and irradiates a biological measurement unit with excitation light.
The autofluorescence emitted from the measuring unit is two-dimensionally detected by an image fiber, received by a CCD image sensor, and the light intensity A1 near a wavelength of 480 nm and the light intensity A2 near a wavelength of 500 nm are detected. The shift tendency of the peak wavelength of the fluorescence spectrum is obtained from the ratio of the light intensity A2, and is displayed as a fluorescence characteristic image.

The endoscope apparatus according to the first embodiment of the present invention is an endoscope inserted into a site suspected of a lesion of a patient.
100, an illumination unit 110 including a light source that emits white light for normal image observation and excitation light for fluorescence measurement, receives fluorescence generated from the living body measurement unit due to the excitation light during fluorescent display, and A1
A1 / A2 calculation unit 120 for calculating / A2, a comparison unit 130 for comparing the previously stored reference value with the calculated A1 / A2 and outputting a signal corresponding to the comparison result
An image processing unit 140 that performs image processing for displaying the normal image and the comparison result as a visible image, a controller 150 that is connected to each unit and controls operation timing, and processes the normal image information processed by the image processing unit 140. It comprises a monitor 170 for displaying as a visible image and a monitor 180 for displaying the result of comparison.

The endoscope 100 includes a light guide 101, a CCD cable 102, and an image fiber 103 which extend to the distal end. Light guide 101 and CCD
At the distal end of the cable 102, that is, at the distal end of the endoscope 100,
An illumination lens 104 and an objective lens 105 are provided. The image fiber 103 is a silica glass fiber, and has a condenser lens 106 at the tip.

At the tip of the CCD cable 102, a CCD
An image pickup device 107 is connected, and the CCD image pickup device 107 has
A mirror 108 is attached. Light guide 101
Is a multi-component glass fiber white light light guide 10
Excitation light light guide 1a and quartz glass fiber
101b is bundled and integrated into a cable shape,
White light guide 101a and excitation light guide 101b
Is connected to the lighting unit 110. CCD cable
One end of 102 is connected to the image processing unit 140, and one end of the image fiber 103 is connected to the A1 / A2 calculation unit 12
Connected to 0.

The illumination unit 110 includes a white light source 111 for emitting white light L1 for normal image observation, a white light source power supply 112 electrically connected to the white light source 111, and excitation light L1 for fluorescence observation.
And a semiconductor laser power supply 115 electrically connected to the GaN-based semiconductor laser 114.

The A1 / A2 calculation unit 120 includes an excitation light cut filter 121 for cutting a wavelength near the excitation light from the fluorescence L3 passing through the image fiber 103, and a mosaic filter 12 in which two types of optical filters are combined on a mosaic.
3 is an on-chip CCD image sensor 125, and an A / D for digitizing a fluorescence signal received by the CCD image sensor 125
The conversion circuit 126 includes a fluorescent image memory 127 for storing a fluorescent image.
And an A1 / A2 calculation unit 128 for calculating A1 / A2 from the values stored in the fluorescence image memory 127.

The mosaic filter 123 is composed of two types of optical filters 124a and 124b as shown in FIG. 2. The optical filter 124a is a narrow-band filter that transmits light of a predetermined wavelength bandwidth centered on 480 nm. The optical filter 124b is a narrow-band filter that transmits light having a predetermined wavelength band centered at 500 nm. The bandwidths of the narrow band filters are equal, and are set so that the transmission wavelength bands do not overlap.

The comparison unit 130 stores a reference value RA stored in the storage unit 131, A1 / A2 calculated by the A1 / A2 calculation unit 127, and the reference value R stored in the storage unit 131.
A comparison unit 132 is provided for comparing A with A. As the reference value RA, a value previously set based on A1 / A2 calculated from a living tissue that is apparently a normal tissue or a diseased tissue is stored.

The image processing unit 140 is a CCD image pickup device
A / D conversion circuit that digitizes the video signal obtained in 107
141, a normal image memory 142 for storing a digitized normal image signal, and a video signal processing circuit 143 for converting the image signal output from the normal image memory 142 and the comparison result of the comparing unit 132 into a video signal. .

Hereinafter, the operation of the endoscope apparatus having the above-described configuration to which the fluorescent display device according to the present invention is applied will be described. First, the operation of the present endoscope apparatus during normal image observation will be described.

During normal observation, the white light source power supply 112 is driven based on a signal from the controller 150, and the white light source 11
1 emits white light L1. The white light L1 passes through the lens 113
After that, the light enters the white light guide 101a, is guided to the distal end of the endoscope, and is then emitted from the illumination lens 104 to the measurement unit 10.

The reflected light of the white light L1 is condensed by the objective lens 105, reflected by the mirror 108, and
Imaged on 7. The video signal from the CCD image sensor 107 is
After being input to the A / D conversion circuit 141 and digitized, it is stored in the normal image memory 142. The normal image memory
The normal image signal saved by 142 is input to the monitor 170 after DA conversion by the video signal generation circuit 143, and displayed on the monitor 170 as a visible image. The above series of operations is controlled by the controller 150.

Next, the operation at the time of displaying the fluorescence characteristic image by the autofluorescence will be described. The excitation light source power supply 115 is driven based on the signal from the controller 150, and the GaN semiconductor laser 114 emits the excitation light L2 having a wavelength of 410 nm. The excitation light L2 passes through the lens 116, is incident on the excitation light light guide 101b, and is guided to the distal end of the endoscope.
The light is emitted from the illumination lens 104 to the measurement unit 10.

The fluorescent light L3 from the measuring section 10 generated by the irradiation of the excitation light L2 is condensed by the condenser lens 106, is incident on the tip of the image fiber 103, passes through the image fiber 103, and passes through the excitation light cut filter. It is incident on 121. The fluorescent light L3 condensed by the lens 122 passes through the mosaic filter 123 on-chip in the CCD image sensor 125, is received by the CCD image sensor 125, and the video signal from the CCD image sensor 125 is converted into an A / D conversion circuit 126. Is input to
After being converted to digital data, it is stored in the fluorescent image memory 127.

At this time, in the fluorescent image memory 127, the fluorescent image signal transmitted through each optical filter of the mosaic filter 123 is stored in different areas. Therefore, the data of the light intensity A1 near the wavelength band 48 and the data of the light intensity A2 near the wavelength band 500 nm are alternately stored. A1 / A2
The calculating unit 128 calculates A1 / A2 for each area using the data stored in the adjacent areas of the fluorescent image memory 127.

The comparing section 132 compares A1 / A2 of each area calculated by the A1 / A2 calculating section 128 with the reference value RA stored in the storage section 131. For example, when the reference value RA is 1.0, when A1 / A2 is larger than the reference value RA, that is, when the light intensity A1 is larger than the light intensity A2, the peak wavelength is the center wavelength of the wavelength band of the light intensity A1. 480 nm, which is the central wavelength of the wavelength band of the light intensity A2, and 5
It can be considered that the wavelength is shifted to the shorter wavelength side than the wavelength of 490 nm which is the central value of 00 nm. If A1 / A2 is smaller than the reference value RA, the peak wavelength is
It can be considered that the wavelength is shifted to a wavelength longer than 90 nm.

The comparison result is displayed on the monitor 180 as an image. When A1 / A2 is equal to or smaller than the reference value RA,
By changing the display color of the measured area when A2 is larger than the reference value RA, the observer can instantly recognize the tendency of the peak wavelength shift.

As described above, the fluorescence emitted from the measuring section by the irradiation of the excitation light indicates that the wavelength band around 480 nm
A wavelength band near 00 nm is cut out, the ratio A1 / A2 of the light intensity is compared with a reference value RA, and a shift tendency of the peak wavelength, which is a comparison result, is displayed. Even if the ratio of the light intensity of the emitted fluorescence or the light intensity of the entire measurement wavelength band to the light intensity of the predetermined wavelength band does not reflect the tissue property of the measurement unit, information reflecting the tissue property can be displayed. It is possible to improve the reliability of display information.

In this embodiment, the light intensities A1 and A2 are cut out from the wavelength band near 480 nm and around 500 nm. However, the light intensity A1 and A2 are cut out only from around 480 nm as the normal peak wavelength or only around 500 nm as the lesion peak wavelength. May be.

For example, by setting the center wavelength of the light intensity A1 to 475 nm and the center wavelength of A2 to 485 nm,
Since the peak wavelength can be regarded as being closer to 480 nm as A1 / A2 is closer to 1, A1 / A2 is information including a relative shift amount of how far the peak wavelength is from 480 nm. In this case, A1 / A2 may be displayed as it is, without comparing it with a reference value, or each light intensity may be displayed by an additive color mixing method, and the ratio of light intensity may be expressed as a change in the tint of the display screen. And the reliability of information can be further improved. Further, by using a GaN-based semiconductor laser having a wavelength of 410 nm as the excitation light irradiating means, it is possible to reduce the size and cost of the apparatus without any problem in detecting the light intensity.

Next, an endoscope apparatus as a second specific embodiment to which the fluorescent display device according to the present invention is applied will be described. Since the configuration is almost the same as that of the first specific embodiment shown in FIG. 1, only the different elements are indicated by the element numbers in FIG. Note that description of elements equivalent to those of the first embodiment will be omitted unless particularly necessary.

This fluorescent display device irradiates the living body measurement section with excitation light, detects fluorescence emitted from the measurement section two-dimensionally by an image fiber, receives light with a CCD image pickup device, and centers the light at 470 nm. Light intensity B1, 4 in predetermined wavelength band
Light intensity B2 and 4 in a predetermined wavelength band centered on 80 nm
The light intensity B3 in a predetermined wavelength band centered on 90 nm is detected, and the shift tendency of the peak wavelength of the fluorescence spectrum is obtained from the second derivative value corresponding to the second derivative of the light intensities B1, B2 and B3, as a fluorescence characteristic image. To display.

The second derivative calculating unit 200 includes an excitation light cut filter 121 for cutting a wavelength near the excitation light from the fluorescence L3 passing through the image fiber 103, and a mosaic filter 20 in which three types of optical filters are combined on a mosaic.
1 is an on-chip CCD image sensor 125, and an A / D for digitizing a fluorescence signal received by the CCD image sensor 125
The conversion circuit 126 includes a fluorescent image memory 203 for storing a fluorescent image.
And a secondary differential value calculating section 204 for calculating a secondary differential value from the value stored in the fluorescent image memory 203.

The mosaic filter 201 is composed of three types of optical filters 202a, 202b and 202c as shown in FIG. 3. The optical filter 202a is a narrow band filter that transmits a predetermined wavelength band centered at 470 nm. The optical filter 202b is a narrow band filter that transmits a predetermined wavelength band centered on 480 nm, and the optical filter 203c is a narrow band filter that transmits a predetermined wavelength band centered on 490 nm.
The bandwidths of the narrow band filters are equal, and are set so that the transmission wavelength bands do not overlap.

The comparison unit 210 includes a storage unit 211 in which the reference value RB is stored, a secondary differential value calculated by the secondary differential value calculation unit 203, and the reference value R stored in the storage unit 211.
B is provided with a comparing unit 212 for comparing B.

Each unit including the secondary differential value calculation unit 200 and the comparison unit 210 includes a controller 220
Control the operation timing. The reference value RB is a value set based on the second derivative of B1, B2, and B3 calculated in advance from a living tissue that is apparently a normal tissue or a diseased tissue.

Hereinafter, the operation of the endoscope apparatus having the above configuration to which the fluorescent display device according to the present invention is applied when displaying the fluorescent characteristic image will be described.

The fluorescence L 3 emitted from the measuring section 10 irradiated with the excitation light L 2 enters the excitation light cut filter 121 via the image fiber 103. The fluorescent light L3 condensed by the lens 122 passes through the mosaic filter 201 on-chip in the CCD image sensor 125, and is received by the CCD image sensor 125. The video signal from the CCD image sensor 125 is converted into an A / D conversion circuit 126 After being input into the digital image data and converted into digital data, it is stored in the fluorescent image memory 203.

At this time, in the fluorescent image memory 203, the fluorescent image signals transmitted through the respective optical filters of the mosaic filter 201 are stored in different areas. Therefore, 470 nm
Near light intensity B1, 480nm near light intensity B2 and 4
Data of the light intensity B3 near 90 nm is stored in each of the adjacent storage areas.

The secondary differential value calculator 203 calculates the secondary differential value BB based on the following equation using the data stored in the adjacent area of the fluorescent image memory 201.

BB = {(B1-B2) / Δλ− (B2-B3) / Δλ} / Δλ = (B1-2 · B2 + 3B) / Δλ2 where Δλ = 480 nm-470 nm = 490 nm-480 nm
And

The comparator 212 compares the secondary differential value BB calculated for each storage area by the secondary differential value calculator 203 with the storage unit 21.
The reference value RB stored in 1 is compared. For example, 2
When the next differential value BB is smaller than the reference value RB, the peak wavelength is 470 nm which is the center wavelength of the wavelength band of the light intensity B1.
And 490 nm, which is the center wavelength of the wavelength band of the light intensity B3, has a high probability.

The result of the comparison is displayed on the monitor 180 as an image. When B1 / B2 is equal to or less than the reference value RB,
By changing the display color of the measured area when B2 is larger than the reference value RB, the observer can instantly recognize the tendency of the shift of the peak wavelength.

As described above, the light intensities in three wavelength bands are detected from the fluorescence emitted from the measurement unit by the irradiation of the excitation light, and the second derivative value BB and the reference value RB are compared. Is displayed, the ratio of the light intensity of the fluorescence emitted from the measurement unit irradiated with the excitation light or the ratio of the light intensity of the entire measurement wavelength band to the light intensity of the predetermined wavelength band is determined by the measurement unit. Even if the organization property is not reflected, the information reflecting the organization property can be displayed, and the reliability of the displayed information can be improved.

In this embodiment, the light intensities B1, B
Although 2 and B3 are cut out from a wavelength band near 480 nm, three wavelength bands may be cut out from a wavelength band including around 480 nm as a normal peak wavelength and around 500 nm as a lesion peak wavelength.

The excitation light irradiation means has a wavelength of 410 nm.
By using the GaN-based semiconductor laser of the present invention, it is possible to reduce the size and cost of the device without hindrance in detecting the light intensity. Next, a third specific embodiment to which the fluorescent display device according to the present invention is applied Will be described.
Since the configuration is almost the same as that of the first specific embodiment shown in FIG. 1, only the different elements are indicated by the element numbers in FIG. Note that description of elements equivalent to those of the first embodiment will be omitted unless particularly necessary.

The present fluorescent display device irradiates the living body measurement section with excitation light, detects fluorescence emitted from the measurement section two-dimensionally by an image fiber, receives light with a CCD image pickup device, and outputs light of 420 nm to 480 nm. Detecting the light intensity C1 of the wavelength band and the light intensity C2 of the wavelength band of 480 nm to 540 nm,
The shift tendency of the peak wavelength of the fluorescence spectrum is obtained from the ratio of the light intensities C1 and C2, and is displayed as a fluorescence characteristic image.

The C1 / C2 calculation unit 300 includes an excitation light cut filter 121 for cutting a wavelength near the excitation light from the fluorescence L3 passing through the image fiber 103, and a mosaic filter 30 in which three types of optical filters are combined on a mosaic.
1 is an on-chip CCD image sensor 125, and an A / D for digitizing a fluorescence signal received by the CCD image sensor 125
The conversion circuit 126 includes a fluorescent image memory 303 for storing a fluorescent image.
And a C1 / C2 calculation unit 304 for calculating C1 / C2 from the values stored in the fluorescence image memory 303.

The mosaic filter 301 is composed of two types of optical filters 302a and 302b arranged similarly to the mosaic filter 123 shown in FIG.
2a is a filter that transmits a wavelength band of 420 nm to 480 nm, and the optical filter 302b is a filter that transmits a wavelength band of 480 nm to 540 nm.

The comparison unit 310 has a storage unit 311 in which the reference value RC is stored, the C1 / C2 calculated in the C1 / C2 calculation unit 303, and the reference value R stored in the storage unit 311.
A comparison unit 312 for comparing C is provided.

Each unit including the C1 / C2 calculation unit 300 and the comparison unit 310 is
Control the operation timing. The reference value RC is a value set in advance based on C1 / C2 calculated from a living tissue that is apparently a normal tissue or a diseased tissue.

Hereinafter, the operation of the endoscope apparatus having the above configuration to which the fluorescent display device according to the present invention is applied when displaying the fluorescent characteristic image will be described.

The fluorescence L 3 emitted from the measuring section 10 irradiated with the excitation light L 2 enters the excitation light cut filter 121 via the image fiber 103. The fluorescent light L3 condensed by the lens 122 passes through the mosaic filter 301 on-chip in the CCD image sensor 125, and is received by the CCD image sensor 125. The video signal from the CCD image sensor 125 is converted into an A / D conversion circuit 126 After being input into the digital image data and converted into digital data, the image data is stored in the fluorescent image memory 303.

At this time, in the fluorescent image memory 303, the fluorescent image signal transmitted through each optical filter of the mosaic filter 301 is stored in different areas. Therefore, the light intensity C1 in the wavelength band of 420 nm to 480 nm, the wavelength band of 480 nm
The light intensity C2 of 540 nm is stored in the storage area alternately. The C1 / C2 calculation unit 303 calculates C1 / C2 using data stored in an adjacent area of the fluorescent image memory 301.

The comparing section 312 compares the C1 / C2 calculated for each storage area by the C1 / C2 calculating section 303 with the reference value RC stored in the storing section 311. For example, when setting the reference value RC, C1 / C determined in advance from a normal tissue
2 is 1.0, and C1 /
If the representative value of C2 is 0.6, as the reference value RC,
By setting 0.8, C1 / C2 becomes the reference value RC.
If it is larger, it can be considered that the peak wavelength of the fluorescence emitted from the measuring unit 10 is shifted to the peak wavelength side of the fluorescence emitted from the normal tissue, and if C1 / C2 is smaller than the reference value RC. It can be considered that the peak wavelength of the fluorescence emitted from the measurement unit 10 is shifted to the peak wavelength side of the fluorescence emitted from the diseased tissue.

The comparison result is displayed on the monitor 180 as an image. When C1 / C2 is equal to or less than the reference value RC,
By changing the display color of the measured area in the case where C2 is larger than the reference value RC, the observer can instantly recognize the shift tendency of the peak wavelength.

As described above, the wavelength band from 420 nm to 480 nm is obtained from the fluorescence emitted from the measurement unit by the irradiation of the excitation light.
Light intensity C1 and light intensity C in the wavelength band of 480 nm to 540 nm
2 is obtained, C1 / C2, which is the ratio of the two, is compared with the reference value RC, and the shift tendency of the peak wavelength, which is the comparison result, is displayed, whereby the fluorescence emitted from the measurement unit irradiated with the excitation light is obtained. Even when the light intensity or the ratio of the light intensity of the entire measurement wavelength band to the light intensity of the predetermined wavelength band does not reflect the tissue property of the measurement unit, information reflecting the tissue property can be displayed. Further, when detecting the light intensity, since the wavelength band cut out from the fluorescence is wide, a large amount of light can be received, so that the detection accuracy is improved and the reliability of the display information can be further improved.

In the present embodiment, C1 / C2 is compared and displayed as to whether it is larger or smaller than reference value RC. However, such a comparison is not always necessary.

In general, the spectrum intensity of the fluorescence emitted from the living tissue is different from the short wavelength side around the peak wavelength,
It can be considered that the long wavelength side is almost a target waveform,
The closer C1 / C2 is to 1, the closer the peak wavelength can be to 480 nm. Therefore, C1 / C2 becomes information including the relative shift amount of how far the peak wavelength is from 480 nm. Therefore, C1 / C2 can be displayed as it is without comparing it with the reference value, or each light intensity can be displayed by the additive color mixing method, and the ratio of the light intensity can be expressed as a change in the tint of the display screen. In this case, information including the relative shift amount of the peak wavelength can be displayed, and the reliability of the information can be further improved.

Next, an endoscope apparatus as a fourth specific embodiment to which the fluorescent display device according to the present invention is applied will be described. Since the configuration is almost the same as that of the first specific embodiment shown in FIG. 1, only the different elements are indicated by the element numbers in FIG. Note that description of elements equivalent to those of the first embodiment will be omitted unless particularly necessary.

The present fluorescent display device irradiates the living body measurement section with excitation light, detects fluorescence emitted from the measurement section two-dimensionally by an image fiber, receives light with a CCD image pickup device, and receives a wavelength of about 480 nm. Band light intensity D1 and 420
The light intensity D2 in the wavelength band of nm to 550 nm is detected, the shift tendency of the peak wavelength of the fluorescence spectrum is determined from the ratio of the light intensities D1 and D2, and the result is displayed as a fluorescence characteristic image.

The mosaic filter 301 provided in the D1 / D2 calculation unit 300 includes a narrow band filter that transmits a wavelength band near 480 nm and a filter that transmits a wavelength band of 420 nm to 550 nm.

The comparing unit 310 stores the reference value RD in the storage unit 311, the D1 / D2 calculated in the D1 / D2 calculation unit 403, and the reference value R stored in the storage unit 411.
A comparison unit 412 for comparing D with D is provided.

Each unit including the D1 / D2 calculation unit 400 and the comparison unit 410 is provided with a controller 420
Control the operation timing. The reference value RD is a value set based on D1 / D2 calculated in advance from a living tissue that is apparently a normal tissue or a diseased tissue.

The operation of the endoscope apparatus having the above-described configuration to which the fluorescent display device according to the present invention is applied when displaying the fluorescent characteristic image will be described.

The fluorescence L 3 emitted from the measuring section 10 irradiated with the excitation light L 2 enters the excitation light cut filter 121 via the image fiber 103. The fluorescence L3 condensed by the lens 122 passes through the mosaic filter 401 on-chip in the CCD image sensor 125, and is received by the CCD image sensor 125. The image signal from the CCD image sensor 125 is converted into an A / D conversion circuit 126 After being input into the digital image data and converted into digital data, it is stored in the fluorescent image memory 403.

At this time, in the fluorescent image memory 403, the fluorescent image signals transmitted through the respective optical filters of the mosaic filter 401 are stored in different areas. Therefore, 480 nm
The nearby light intensity D1 and the light intensity D2 in the wavelength band of 420 nm to 550 nm are stored alternately in the storage area. The D1 / D2 calculation unit 403 calculates D1 / D2 using data stored in an adjacent area of the fluorescence image memory 401.

The comparison unit 412 compares the D1 / D2 calculated for each storage area by the D1 / D2 calculation unit 403 with the reference value RD stored in the storage unit 411.

For example, when setting the reference value RD, the representative value of D1 / D2 previously obtained from normal tissue is 0.1, and the representative value of D1 / D2 obtained from diseased tissue is 0.06.
Then, by setting 0.08 as the reference value RD, if D1 / D2 is larger than the reference value RD, the peak wavelength of the fluorescence emitted from 10 from the measurement unit becomes the fluorescence emitted from the normal tissue. If D1 / D2 is smaller than the reference value RD, the peak wavelength of the fluorescence emitted from the measuring unit 10 is the peak wavelength of the fluorescence emitted from the diseased tissue. Can be considered as shifting to the side.

The comparison result is displayed on the monitor 180 as an image. When D1 / D2 is equal to or less than the reference value RD,
By changing the display color of the measured area when D2 is larger than the reference value RD, the observer can instantly recognize the tendency of the shift of the peak wavelength.

As described above, the light intensity D1 in the vicinity of 480 nm and the light intensity D2 in the wavelength band of 420 nm to 550 nm are obtained from the fluorescence emitted from the measurement unit by the irradiation of the excitation light, and the ratio D1 / D2 of both is obtained. By displaying the shift tendency of the peak wavelength as a comparison result in comparison with the reference value RD, the light intensity of the fluorescence emitted from the measurement unit irradiated with the excitation light or the light intensity of the entire measurement wavelength band and the predetermined wavelength Even when the ratio of the light intensity in the band does not reflect the tissue property of the measurement unit, information reflecting the tissue property can be displayed. Further, when detecting the light intensity D2, since the wavelength band cut out from the fluorescence is wide, a large amount of light can be received, so that the detection accuracy is improved and the reliability of the display information can be further improved.

In the present embodiment, D1 / D2 is compared and displayed as to whether it is larger or smaller than reference value RD, but such a comparison is not always necessary.

In general, it can be considered that the larger the value of D1 / D2, the closer the peak wavelength is to 480 nm. Therefore, D1 / D2 is information including the relative shift amount of how far the peak wavelength is from 480 nm. Therefore, D1 / D2 can be displayed as it is without comparing it with the reference value, or each light intensity can be displayed by the additive color mixing method, and the ratio of light intensity can be expressed as a change in the tint of the display screen. In this case, information including the relative shift amount of the peak wavelength can be indicated, and the reliability of the information can be further improved.

The wavelength band for detecting the light intensity D1 is as follows.
Although the wavelength band including 480 nm is set as the normal peak wavelength, a wavelength band including 500 nm as the lesion peak wavelength can also be set. In this case, the shift tendency of the peak wavelength based on the wavelength of 500 nm can be detected. .

Next, an endoscope apparatus according to a fifth specific embodiment to which the fluorescent display device according to the present invention is applied will be described. Since the configuration is almost the same as that of the first specific embodiment shown in FIG. 1, only the different elements are indicated by the element numbers in FIG. Note that description of elements equivalent to those of the first embodiment will be omitted unless particularly necessary.

The present fluorescent display device irradiates the living body measurement section with excitation light, detects fluorescence emitted from the measurement section two-dimensionally by an image fiber, receives the fluorescence with a CCD image pickup device, and receives a wavelength around 480 nm. Band light intensity E1, 420 nm ~
The light intensity E2 of the 550 nm wavelength band and the light intensity E3 of the entire measurement wavelength band are detected, and the ratio of the light intensity E1 and the light intensity E2 as the peak wavelength shift tendency, the light intensity E2 as the blue band light intensity, The information based on the ratio between the light intensity E2 and the light intensity E3 as the ratio between the peak light intensity and the light intensity in the entire measurement wavelength band is displayed as a fluorescence characteristic image.

In the comparison value calculation unit 500, the excitation light cut filter 121 for cutting the wavelength near the excitation light from the fluorescence L3 passing through the image fiber 103, and the mosaic filter 501 in which three kinds of optical filters are combined on a mosaic are turned on. A CCD image sensor 125 chipped, an A / D conversion circuit 126 for digitizing a fluorescent signal received by the CCD image sensor 125, a fluorescent image memory 503 for storing a fluorescent image, and a value stored in the fluorescent image memory 503. A comparison value calculator 504 for calculating E1 / E2 and E2 / E3 is provided.

The mosaic filter 501 includes a narrow band filter that transmits a wavelength band near 480 nm,
It is composed of a filter that transmits the wavelength band of ５550 nm and a blank that transmits the entire wavelength band. The three filters are arranged similarly to the mosaic filter 201 shown in FIG.

The comparing unit 510 includes the reference values RE1, RE
A storage unit 511 storing 2 and R3, and a comparison unit 512 for comparing the comparison value calculated by the comparison value calculation unit 503 with the reference value stored in the storage unit 511 are provided.

The operation timing of each unit including the comparison value calculation unit 500 and the comparison unit 510 is controlled by the controller 520.

The reference values RE1, RE2 and RE3 are E1 / E2, E2 and E2 / E3 calculated in advance from a living tissue which is apparently a normal tissue or a diseased tissue.
It is a value set based on.

Hereinafter, the operation of the endoscope apparatus having the above-described configuration to which the fluorescent display device according to the present invention is applied when the fluorescent characteristic image is displayed will be described.

The fluorescent light L3 emitted from the measuring section 10 irradiated with the excitation light L2 enters the excitation light cut filter 121 via the image fiber 103. The fluorescent light L3 condensed by the lens 122 passes through the mosaic filter 501 on-chip in the CCD image sensor 125, is received by the CCD image sensor 125, and the video signal from the CCD image sensor 125 is converted into an A / D conversion circuit 126. After being input to the digital camera and converted into digital data, the digital data is stored in the fluorescent image memory 503.

At this time, in the fluorescent image memory 503, the fluorescent image signal transmitted through each optical filter of the mosaic filter 501 is stored in different areas. Therefore, 480 nm
The light intensity E1 in the vicinity, the light intensity E2 in the wavelength band of 420 nm to 550 nm, and the light intensity E3 in the entire measurement wavelength band are stored in adjacent storage areas.

In the comparison value calculation unit 503, the fluorescence image memory 50
Using data stored in one adjacent storage area, E1 / E2 and E2 / E3 are calculated and stored for each area. At the same time, E2 is also stored.

In the comparison section 512, E1 / E2, E2 and E2 for each storage area stored in the comparison value calculation section 503 are stored.
/ E3 and the reference value RE1, stored in the storage unit 511,
RE2 and RE3 are compared.

When E1 / E2 is equal to or greater than the reference value RE1, the characteristics of the fluorescence emitted from the measurement part are close to the characteristics of the fluorescence emitted from the normal tissue by comparison based on the tendency of the shift of the peak wavelength. Is considered. When E2 is equal to or greater than the reference value RE2, the characteristics of the fluorescence emitted from the measurement unit are considered to be close to the characteristics of the fluorescence emitted from the normal tissue by comparison based on the blue band light intensity. E
When 2 / E3 is equal to or more than the reference value RE3, the characteristics of the fluorescence emitted from the measurement unit are emitted from the normal tissue by comparison based on the ratio between the peak light intensity and the light intensity in the entire measurement wavelength band. Is considered to be close to the characteristics of the fluorescence.

The comparison result is displayed on the monitor 180 as an image. In all three types of comparisons, the color of the fluorescence emitted from the measurement part is considered to be close to the characteristic of the fluorescence emitted from the normal tissue, and is displayed in a different color from the other cases. As a result, it is possible to display information that takes into account the three characteristics of the measured fluorescence spectrum intensity, spectrum waveform, and peak wavelength shift tendency, and the observer can instantly recognize the fluorescence characteristics of the measurement unit. .

Further, as described above, in all three comparison results, only when it is considered that the characteristics of the fluorescence emitted from the measurement section are close to the characteristics of the fluorescence emitted from the normal tissue, a different display color, that is, a normal display color is obtained. By displaying in a display color representing the tissue, the possibility of erroneous display that the fluorescence emitted from the diseased tissue is close to the fluorescence emitted from the normal tissue is reduced.
For this reason, it is suitable when the measurement unit is highly likely to be a diseased tissue, for example, when performing fluorescence detection in a living body having a preexisting condition.

Further, the excitation light irradiation means has a wavelength of 410 nm.
The use of the GaN-based semiconductor laser described above makes it possible to reduce the size and the cost of the device without hindrance to the detection of light intensity. Further, in the present embodiment, the peak light intensity in the wavelength band near 480 nm The light intensity was detected, and the ratio between the light intensity and the light intensity in the entire measurement wavelength band was obtained.
Instead of the light intensity in the wavelength band near 480 nm, the light intensity in the wavelength band near 630 nm or 700 nm may be detected, and the ratio between the light intensity and the light intensity in the entire measurement wavelength band may be calculated. In this case, the mosaic filter 501
What is necessary is just to add a filter which transmits light of a wavelength band near 630 nm or 700 nm.

As a modification of the present embodiment, the characteristics of the fluorescence emitted from the measurement part were considered to be close to the characteristics of the fluorescence emitted from the normal tissue in at least one of the three types of comparison. There is a method of changing the display color between the case and the other cases. In this case, the possibility that the fluorescence emitted from the normal tissue is erroneously displayed as being close to the fluorescence emitted from the diseased tissue is low, and therefore, for example, this is suitable when the measurement unit is unlikely to be the diseased tissue. .

Further, in the present embodiment, information based on the peak wavelength shift tendency, the blue band light intensity, and the ratio of the peak wavelength intensity to the total measured wavelength intensity is displayed. , And information based on the ratio of the peak wavelength intensity to the total measured wavelength intensity may be displayed. In this modified example, the configuration of the comparison unit can be simplified, and information that takes into account the shift tendency of the measured peak wavelength of fluorescence and the spectral waveform characteristics can be displayed with a simplified configuration.

As another example, a method of displaying information based on the shift tendency of the peak wavelength and the blue band light intensity can be considered. In this modified example, the optical system for measuring the light intensity in the entire wavelength band is unnecessary, so that the optical system in addition to the comparison unit can be simplified, and the shift tendency of the measured fluorescence peak wavelength and the spectral intensity characteristics are taken into account. Information can be displayed in a more simplified configuration.

In the first to fifth embodiments, the comparison processing is performed for each pixel in each comparison unit, but not for each pixel, but for each pixel corresponding to the binning processing of the CCD image sensor. The comparison processing may be performed, or the comparison may be performed in units of pixel regions in an arbitrary range desired by the measurer. Alternatively, the comparison can be performed only in the area specified by the measurer, or the comparison can be performed by thinning out pixels as appropriate.

If there is an area for which comparison processing has not been performed, the comparison area can be clearly displayed by displaying the display color of the area in a predetermined color. For example, when the comparison pixels are thinned out, complementary display is performed based on the comparison result of the vicinity.

Further, a mosaic filter having a blank transmitting all wavelength bands is provided by a CCD built in an endoscope.
If it is arranged on the front surface of the image pickup device, this CCD image pickup device can be used for both normal image detection and fluorescence detection.

If a CCD image pickup device having the above-described mosaic filter on-chip is disposed at the end of the endoscope, it can be used for both normal image detection and fluorescence detection.

Next, an endoscope apparatus according to a sixth specific embodiment to which the fluorescent display device according to the present invention is applied will be described with reference to FIGS. FIG. 4 is a schematic configuration diagram of an endoscope apparatus to which the fluorescent display device according to the present invention is applied. From the fluorescence emitted from one point of the site, the light intensity F1 in the wavelength band near 480 nm and the light intensity F2 in the wavelength band near 500 nm are detected, and the peak wavelength of the fluorescence spectrum is shifted from the ratio of the light intensity F1 to the light intensity F2. A trend is obtained and displayed.

[0132] The endoscope apparatus according to the embodiment of the present invention is an endoscope 600 inserted into a site suspected of a lesion of a patient.
An illumination unit 610 including a light source that emits white light for normal image observation and excitation light for fluorescence measurement, an optical path separation unit 620 that separates the optical path of excitation light and measured fluorescence, generated from the biological measurement unit by the excitation light during fluorescent display. Fluorescent light is received and F1 /
The F1 / F2 calculation unit 630 that calculates F2 compares the reference value stored in advance with the calculated F1 / F2,
A comparison unit 640 that outputs a signal according to the comparison result, an image processing unit 650 that performs image processing for displaying a fluorescence characteristic, which is a shift tendency of the peak wavelength of the normal image and the fluorescence spectrum, as a visible image, and is connected to each unit. , A controller 660 for controlling operation timing, a monitor 170 for displaying normal image information processed by the image processing unit 650 as a visible image, a monitor 180 for displaying fluorescence characteristics, and a quartz fiber 690 for guiding excitation light and fluorescence.
It is composed of

The endoscope 600 comprises a light guide 601, a CCD cable 602 and a quartz fiber
90 has a forceps port 603 therethrough. At the distal end of the light guide 601 and the CCD cable 602, that is, at the distal end of the endoscope 600, an illumination lens 604 and an objective lens are provided.
605. At the tip of the CCD cable 602,
The CCD image sensor 606 is connected to the CCD image sensor 606.
, A mirror 607 is attached. Light guide
One end of 601 is connected to the lighting unit 610, and one end of the CCD cable 602 is connected to the image processing unit 650.

The illumination unit 610 includes a white light source 611 that emits white light L4 for normal image observation, a white light source power supply 612 electrically connected to the white light source 611, and excitation light L5 for fluorescence observation.
A GaN-based semiconductor laser 614 as an excitation light source that emits light, and a semiconductor laser power supply 615 electrically connected to the GaN-based semiconductor laser 614.

The optical path separating section 620 is a GaN semiconductor laser 61
4 emits the excitation light L5 into the quartz fiber 690, and conversely, the fluorescent light L6 passing through the quartz fiber 690
A 1 / F2 calculation unit 630 is provided with a dichroic mirror 621 for transmission.

The F1 / F2 calculation unit 630 includes an excitation light cut filter 631 for cutting the wavelength near the excitation light from the fluorescence L6 passing through the quartz fiber 690, and the excitation light cut filter.
A switching filter 633 for cutting out light in a predetermined wavelength band near 480 nm or a predetermined wavelength band near 500 nm from the fluorescent light L6 transmitted through 631, a filter rotating device 635 for rotating the switching filter 633, and 480 nm transmitted through the switching filter 633.
A photodetector 636 for measuring the light intensity F1 in the vicinity of the predetermined wavelength band and the light intensity F2 in the predetermined wavelength band of 500 nm, a measurement data memory 637 for storing the measurement data stored in the photodetector 636, and F1 / F2. An F1 / F2 calculator 638 for calculating is provided.

As shown in FIG. 5, the switching filter 633 has a narrow band optical filter 634a for transmitting light in a predetermined wavelength band centered at 480 nm and a narrow band optical filter 634a for transmitting light in a predetermined wavelength band centered at 500 nm. Optical filters
634b.

The comparing unit 640 stores a reference value RA stored in the storage unit 641 and the F1 / F2 calculated by the F1 / F2 calculation unit 637 and the reference value R stored in the storage unit 641.
A comparison unit 642 for comparing with F is provided.

[0139] The reference value RF is set based on F1 / F2 obtained in advance from a living tissue that has been recognized as a normal tissue or a diseased tissue, and is stored in the storage unit 641.

An image processing unit 650 is a CCD image pickup device
A / D conversion circuit to digitize the video signal obtained by 606
651, a normal image memory 652 for storing a digitized normal image signal, and a video signal processing circuit 653 for converting the image signal output from the normal image memory 652 and the comparison result of the comparing section 642 into a video signal. .

Hereinafter, the operation of the endoscope apparatus having the above configuration to which the fluorescent display device according to the present invention is applied will be described. First, the operation of the present endoscope apparatus during normal image observation will be described. During normal observation, the white light source power supply 612 is driven based on the signal from the controller 660, and the white light source
Is injected. The white light L4 is incident on the light guide 601 via the lens 613, and is guided to the distal end of the endoscope.
The light is emitted from the illumination lens 604 to the observation unit 20 including the measurement unit 11.

The reflected light of the white light L4 is condensed by the objective lens 605, reflected by the mirror 607 at a right angle in the optical path, and is imaged on the CCD image sensor 606. CCD image sensor
The video signal from 606 is input to the A / D conversion circuit 651, digitized, and stored in the normal image memory 652. The normal image signal stored in the normal image memory 652 is input to the monitor 670 after DA conversion by the video signal generation circuit 653, and is displayed on the monitor 670 as a visible image. The above series of operations is controlled by the controller 660.

Next, the operation at the time of displaying the fluorescent characteristic will be described. The excitation light source power supply 615 is driven based on the signal from the controller 660, and the excitation light L5 having a wavelength of 410 nm is emitted from the GaN-based semiconductor laser 614. The excitation light L5 passes through the lens 616 and travels to the dichroic mirror 621. Excitation light L5 reflected by dichroic mirror 621
Is incident on the quartz fiber 690 by the lens 622,
The light is guided to the vicinity of the measurement unit 11 through the forceps port 603 of the endoscope, and is irradiated from the tip of the quartz fiber 690 to the measurement unit 11.

The fluorescent light L6 from the measuring section 11 generated by irradiating the excitation light L5 is incident on the tip of the quartz fiber 690, and travels through the quartz fiber 690 and the lens 622 to the dichroic mirror 621. The dichroic mirror 621 has a structure for transmitting a light beam incident from the left side in the drawing. The dichroic mirror
The fluorescence L6 transmitted through 621 passes through the excitation light cut filter 631 and the lens 632, and enters the switching filter 633. The excitation light cut filter 631 has a wavelength of 420 nm.
This is a long-pass filter that transmits all the fluorescence described above. Since the wavelength of the excitation light L5 is 410 nm, the excitation light L5 reflected by the measurement unit 11 is cut by the excitation light cut filter 631 and does not enter the switching filter 633.

Under the control of the controller 660, the filter rotating device 635 is driven, and the fluorescent light L6 is sequentially passed through the optical filter.
After passing through 634a or 634b, the light enters the photodetector 636. At the same time, the measurement data memory
Under the control from 660, the data of the fluorescence transmitted through the optical filter 634a is stored in a predetermined area in the measurement data memory 637, and the data of the fluorescence transmitted through the optical filter 634b is stored in a different area.

The F1 / F2 calculator 638 calculates the F1 / F2 from the fluorescence intensity data stored in the measurement data memory 637.
2 is calculated.

The comparison section 642 compares the reference value RF stored in the storage section 641 with F1 / F2 calculated by the F1 / F2 calculation section 638.

The comparison result is displayed on the monitor 180.

Therefore, as described above, the wavelength band near 480 nm from the fluorescence guided by the quartz
Cut out the wavelength band near 0 nm, calculate the light intensity ratio F1 / F2, compare the light intensity ratio F1 / F2 with the reference value RA, and display the peak wavelength shift tendency as the comparison result. By doing so, information with improved reliability can be displayed.

Further, in the present apparatus, the distance between the measurement site and the tip of the quartz fiber 690 can be reduced, sufficient light intensity can be obtained even if the detection bandwidth is narrowed, and the reliability is further improved. Information can be displayed.

The excitation light irradiation means has a wavelength of 410 nm.
By using the GaN-based semiconductor laser described above, it is possible to reduce the size and cost of the device without any problem in detecting the light intensity.

The structure of this embodiment is different from the first embodiment in that the quartz fiber, the switching filter, and the photodetector used in the present embodiment are replaced with the image fiber, the mosaic filter, and the CCD image pickup device. By having it, it is applicable to the second to fifth embodiments, and substantially the same effect is obtained except that fluorescence is detected from only one point of the measurement unit instead of two-dimensional detection of fluorescence. Is obtained.

The monitor used in each of the devices according to the first to sixth embodiments has a monitor 170 for displaying normal image information and a monitor 180 for displaying fluorescent characteristics, which are separate from each other. One monitor can be shared. At this time, the display may be switched automatically in a time-series manner, or may be arbitrarily switched by a measurer using switching means.

Although the GaN-based semiconductor laser and the white light source are configured separately, a single light source can be used for both excitation light and white light by using an appropriate optical transmission filter.

It goes without saying that changes within the basic structure of the present invention, such as separation of the fiber for guiding the excitation light and the fiber for guiding the white light, and the acquisition of a normal image with an image fiber are possible. No.

In each of the above embodiments, a case is described in which information corresponding to the tendency of the shift of the peak wavelength of the light intensity in the autofluorescence of the living body is displayed without injecting the fluorescent diagnostic agent into the living body. However, the present invention can be similarly applied to a fluorescent display device which displays information according to a shift tendency of the peak wavelength of the light intensity in the fluorescence of the drug emitted from the living body into which the fluorescent diagnostic agent has been injected. In this case, the living body in which the fluorescent diagnostic agent has been injected in advance is irradiated with excitation light having a predetermined excitation wavelength, and the agent fluorescence emitted from the living body is detected, and the peak wavelength of light intensity is shifted according to the shift tendency. Display information.

[Brief description of the drawings]

FIG. 1 is a schematic configuration diagram of an endoscope apparatus as a first specific embodiment to which a fluorescent display device according to the present invention is applied.

FIG. 2 is a schematic configuration diagram of a mosaic filter used in the endoscope apparatus according to the first specific embodiment.

FIG. 3 is a schematic configuration diagram of a mosaic filter used in an endoscope apparatus according to a second specific embodiment.

FIG. 4 is a schematic configuration diagram of an endoscope apparatus which is a sixth specific embodiment to which the fluorescent display device according to the present invention is applied.

FIG. 5 is a schematic configuration diagram of a switching filter used in the endoscope apparatus according to the sixth specific embodiment.

FIG. 6 is an explanatory diagram showing an intensity distribution of a fluorescence spectrum of autofluorescence.

FIG. 7 is an explanatory diagram showing an intensity ratio distribution of a fluorescence spectrum of autofluorescence.

FIG. 8 is an explanatory diagram showing a distribution of peak wavelength frequencies around 480 nm of autofluorescence spectra emitted from normal tissues and diseased tissues.

[Explanation of symbols]

 10,11 Measuring unit 20 Observing unit L2, L4 White light L3, L5 Excitation light L1, L6 Fluorescence 100,600 Endoscope 101,601 Light guide 102,602 CCD cable 107,125,606 CCD image sensor 110,610 Lighting unit 111,611 White light source 114,614 GaN semiconductor laser 120 A1 / A2 calculation unit 121,631 Excitation light cut filter 123,201,301,401,501 Mosaic filter 124a, 124b Optical filter 127,203,303,403,503 Fluorescence image memory 128 A1 / A2 calculation unit 130,210,310,410,520,640 Comparison unit 131,211,311,411,511,641, Storage unit 132,212,312,142,320,320, Processing unit 170,180 Monitor 200 Secondary differential value calculation unit 203 Secondary differential value calculation unit 300 C1 / C2 calculation unit 303 C1 / C2 calculation unit 400 D1 / D2 calculation unit 403 D1 / D2 calculation unit 500 Comparison value calculation unit 503 Comparison value calculation unit 630 F1 / F2 calculation unit 633 Switching filter 634a, 634b Optical filter 636 Photodetector 637 Measurement data memory 638 F1 / F2 calculation unit 690 Quartz fiber

Continuation of the front page F term (reference) 2G043 AA03 BA16 CA03 EA01 FA01 GA08 GA21 GB21 HA01 HA05 JA03 KA02 KA05 KA09 LA03 NA01 NA06 2H040 GA02 GA11 4C061 AA00 BB02 CC07 DD00 LL03 NN01 QQ04 RR04 RR14 SS21 SS22 WW17

Claims (17)

[Claims] 1. Irradiation with a predetermined normal peak wavelength or excitation light which is a representative value of a peak wavelength at which the light intensity becomes maximum in a spectral intensity distribution of fluorescence emitted from a normal tissue of a living body irradiated with the excitation light. Based on a previously determined lesion peak wavelength which is a representative value of the peak wavelength at which the light intensity is maximum in the spectral intensity distribution of the fluorescence emitted from the diseased tissue of the living body, the measurement unit of the living body irradiated with the excitation light A fluorescent display method comprising: obtaining a shift tendency of a peak wavelength of light intensity in fluorescence emitted from a fluorescent substance; and displaying information according to the shift tendency of the peak wavelength. 2. Exciting light irradiating means for irradiating the measuring part of the living body with the exciting light, and a peak wavelength having a maximum light intensity in a spectral intensity distribution of fluorescence emitted from a normal tissue of the living body irradiated with the exciting light. A pre-determined normal peak wavelength which is a representative value or a pre-determined lesion which is a representative value of a peak wavelength at which the light intensity is maximized in a spectral intensity distribution of fluorescence emitted from a diseased tissue of a living body irradiated with excitation light. Based on the peak wavelength, the fluorescent light for obtaining information according to the shift tendency of the peak wavelength of the light intensity in the fluorescence emitted from the measurement unit by the irradiation of the excitation light, and displaying the information according to the shift tendency of the peak wavelength A fluorescent display device comprising display means. 3. The fluorescent display device according to claim 2, wherein the normal peak wavelength is around 480 nm, and the lesion peak wavelength is around 500 nm. 4. The apparatus according to claim 1, wherein said fluorescent display means is configured to firstly detect the fluorescence emitted from the measurement unit in a peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength.
First light intensity detecting means for detecting light intensity in a wavelength band of: and second light intensity detecting means for detecting light intensity in a second wavelength band different from the first wavelength band within the peak wavelength band. And, as information according to the shift tendency of the peak wavelength of the light intensity, according to a ratio of the light intensity detected by the first light intensity detection unit to the light intensity detected by the second light intensity detection unit. 4. The fluorescent display device according to claim 2, further comprising a display unit for displaying the information. 5. The first light intensity detection means and the second light intensity detection means, wherein only the wavelength band near the normal peak wavelength or only the wavelength band near the lesion peak wavelength is used for the first wavelength band. The fluorescent display device according to claim 4, wherein the light intensity and the light intensity of the second wavelength band are detected. 6. The fluorescent display means is configured to perform third from fluorescence emitted from the measurement unit in a peak wavelength band including the vicinity of the normal peak wavelength and the vicinity of the lesion peak wavelength.
A third light intensity detecting means for detecting the light intensity of the wavelength band of the fourth wavelength band; and a fourth light intensity detecting means of detecting the light intensity of the fourth wavelength band on the longer wavelength side of the third wavelength band within the peak wavelength band. Light intensity detecting means for detecting the light intensity of a fifth wavelength band on the longer wavelength side than the fourth wavelength band within the peak wavelength band, and a peak of the light intensity As information according to the wavelength shift tendency, the light intensity detected by the third light intensity detecting means, the light intensity detected by the fourth light intensity detecting means, and the fifth
4. A fluorescent display device according to claim 2, further comprising display means for displaying information corresponding to a second derivative of the spectrum intensity distribution obtained from the light intensity detected by the light intensity detection means. . 7. The third light intensity detecting means, the fourth light intensity detecting means and the fifth light intensity detecting means, wherein only the wavelength band near the normal peak wavelength or the wavelength near the lesion peak wavelength is used. 7. The fluorescent display device according to claim 6, wherein the light intensity of the first wavelength band, the light intensity of the second wavelength band, and the fluorescence intensity of the third wavelength band are detected only from the bands. 8. The peak wavelength band is from 470 nm to 52
8. A wavelength band of 0 nm, wherein the wavelength band is 0 nm.
The fluorescent display device according to claim 1. 9. The light intensity display device according to claim 6, wherein said fluorescence display means includes a light intensity in a sixth wavelength band, which is a wavelength band shorter than a predetermined wavelength near the normal peak wavelength or near the lesion peak wavelength from the fluorescence emitted from the measurement unit. Sixth light intensity detecting means for detecting light intensity; seventh light intensity detecting means for detecting light intensity in a seventh wavelength band which is a wavelength band longer than the predetermined wavelength; and a peak wavelength of the light intensity. Display means for displaying information corresponding to a ratio between the light intensity detected by the sixth light intensity detection means and the light intensity detected by the seventh light intensity detection means, as information corresponding to the shift tendency of The fluorescent display device according to claim 2, further comprising: 10. The lower limit wavelength of the sixth wavelength band is 42.
0 nm, and the upper limit wavelength of the seventh wavelength band is 550 nm.
The fluorescent display device according to claim 9, wherein: 11. An eighth light intensity detecting means for detecting a light intensity in an eighth wavelength band including the vicinity of the normal peak wavelength or the vicinity of the lesion peak wavelength from the fluorescence emitted from the measurement unit. Detecting means; ninth light intensity detecting means for detecting light intensity in a ninth wavelength band including the eighth wavelength band; and information corresponding to the shift tendency of the peak wavelength of the light intensity. And display means for displaying information corresponding to a ratio between the light intensity detected by the light intensity detection means and the light intensity detected by the ninth light intensity detection means. Or the fluorescent display device according to 3. 12. The lower limit wavelength of the ninth wavelength band is 42.
The fluorescent display device according to claim 11, wherein the wavelength is 0 nm and the upper limit wavelength is 550 nm. 13. The blue-band light intensity detecting unit detects, as the blue-band light intensity, the light intensity in a predetermined band near a normal peak wavelength from the fluorescence emitted from the measurement unit by the irradiation of the excitation light. Comprising a detecting means, the display means, the shift tendency of the peak wavelength of light intensity,
13. The fluorescent display device according to claim 2, wherein information based on the blue band light intensity is displayed. 14. The fluorescence display means, wherein all light intensity detection means for detecting light intensity in all measurement wavelength bands from fluorescence emitted from the measurement section by irradiation of the excitation light, and peak light from the fluorescence Intensity around 480 nm, 6
A peak light intensity detecting means for detecting light intensity of one wavelength band in a wavelength band near 30 nm or near 700 nm, wherein the display means comprises: a shift tendency of a peak wavelength of the light intensity; 13. The fluorescent display device according to claim 2, wherein information based on a ratio of light intensities in the entire measurement wavelength band is displayed. 15. The blue-band light intensity detecting unit detects, as the blue-band light intensity, the light intensity in a predetermined band near a normal peak wavelength from the fluorescence emitted from the measurement unit by the irradiation of the excitation light. Detection means; total light intensity detection means for detecting the light intensity of the entire measurement wavelength band from the fluorescence; and a peak light intensity of about 480 nm, 6 from the fluorescence.
A peak light intensity detecting means for detecting the light intensity of one wavelength band in the wavelength band near 30 nm or 700 nm, wherein the display means comprises: a shift tendency of the peak wavelength of the light intensity; 13. The fluorescent display device according to claim 2, wherein information based on the intensity and a ratio between the peak light intensity and the light intensity of the entire measurement wavelength band is displayed. 16. The wavelength of the excitation light is from 380 nm to 42.
16. The semiconductor device according to claim 2, wherein the thickness is 0 nm.
Item 7. The fluorescent display device according to Item 1. 17. The fluorescent display device according to claim 2, wherein said excitation light irradiation means is a GaN-based semiconductor laser.