JP2001106620A5 - - Google Patents

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Publication number
JP2001106620A5
JP2001106620A5 JP1999283793A JP28379399A JP2001106620A5 JP 2001106620 A5 JP2001106620 A5 JP 2001106620A5 JP 1999283793 A JP1999283793 A JP 1999283793A JP 28379399 A JP28379399 A JP 28379399A JP 2001106620 A5 JP2001106620 A5 JP 2001106620A5
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JP
Japan
Prior art keywords
weeks
measured
added
skin
kud
Prior art date
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Pending
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JP1999283793A
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Japanese (ja)
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JP2001106620A (en
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Priority to JP28379399A priority Critical patent/JP2001106620A/en
Priority claimed from JP28379399A external-priority patent/JP2001106620A/en
Publication of JP2001106620A publication Critical patent/JP2001106620A/en
Publication of JP2001106620A5 publication Critical patent/JP2001106620A5/ja
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Description

（実験方法）３週間、５週間、７週間紫外線Ｂを照射したヘアレスマウス（Ｈｒ−／Ｋｕｄ、雌性）の背部皮膚より、小片切り出し、トリプシン処理し、皮膚由来真皮線維芽細胞を採取し、５代継代して用いた。培養条件は、１〜５×１０ 4 個／ｍｌの細胞濃度で、０．３〜１．０ｍｇ／ｍｌの酸可溶性コラーゲンを加え、１０％ＦＢＳを含むイーグルの最小培地で、１．０ｍｇ／ｍｌの濃度で１４日間培養した。毎日、コラーゲンゲルの直径を測定し、その収縮を測定した。 (Experimental method) From the back skin of a hairless mouse (Hr- / Kud, female) irradiated with ultraviolet B for 3 weeks, 5 weeks, and 7 weeks, a small piece was cut out, treated with trypsin, and skin-derived dermal fibroblasts were collected. Passage was used. Culture conditions were as follows: cell concentration of 1-5 × 10 4 cells / ml, acid-soluble collagen of 0.3-1.0 mg / ml was added, and Eagle's minimum medium containing 10% FBS was added at 1.0 mg / ml. For 14 days. Every day, the diameter of the collagen gel was measured and its shrinkage was measured.

JP28379399A 1999-10-05 1999-10-05 Method for discriminating active ingredient of skin preparation for external use Pending JP2001106620A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP28379399A JP2001106620A (en) 1999-10-05 1999-10-05 Method for discriminating active ingredient of skin preparation for external use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP28379399A JP2001106620A (en) 1999-10-05 1999-10-05 Method for discriminating active ingredient of skin preparation for external use

Publications (2)

Publication Number Publication Date
JP2001106620A JP2001106620A (en) 2001-04-17
JP2001106620A5 true JP2001106620A5 (en) 2006-07-13

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ID=17670222

Family Applications (1)

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JP28379399A Pending JP2001106620A (en) 1999-10-05 1999-10-05 Method for discriminating active ingredient of skin preparation for external use

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JP (1) JP2001106620A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7209187B2 (en) * 2019-03-04 2023-01-20 日本メナード化粧品株式会社 Aging evaluation method of skin collagen fiber structure using X-ray diffraction

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