JP2001106608A - Plant growth promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new medicine for raising a plant, useful for the control of symbiosis, and a plant growth promoter having higher effects and containing a cultured product of microorganisms belonging to the genus Pseudomonas as a component. SOLUTION: This plant growth promoter or adjuvant for the plant growth promoter contains polysaccharides obtained from the cultured product of microorganisms belonging to the genus Klebsiella. The plant growth promoter comprises the polysaccharides obtained from the cultured product of the microorganisms belonging to the genus Klebsiella, and the cultured product of microorganisms belonging to the genus Pseudomonas. The microorganism belonging to the genus Klebsiella is exemplified by the microorganisms belonging to Klebsiella pneumoniae or Klebsiella oxytoca. The microorganism belonging to the genus Pseudomonas is exemplified by the microorganisms belonging to Pseudomonas fluorescens.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a Klebsiella (K)
The present invention relates to a plant growth promoter or a plant growth promotion aid containing a polysaccharide obtained from a culture of a microorganism belonging to the genus Lebsiella. Furthermore, the present invention relates to Klebsiella (Kl
polysaccharides obtained from cultures of microorganisms belonging to the genus Ebsiella and Pseudomonas
s) A plant growth promoter comprising a culture of a microorganism belonging to the genus.

[0002]

2. Description of the Related Art Pseudomonas fluorescens (Pseudomonas)
fluorescens) grows in the soil around the plant roots and produces various antibiotics such as piorteolin, 2,4-diacetylphloroglucinol, phenazine carboxylic acid, etc., thereby transforming the host plant from various pathogens. It is believed to be defending. Therefore, research on the mechanism of antibiotic production by the bacterium, improvement of its productivity, and ecology in soil have been important themes. Recently, a high producing strain of P. fluoresc
ens S272 strain was isolated [Z. Yuan et al.
Ferment. Bioeng. 86,6,559-5
63 (1998)], and it has been reported that various stresses such as high alcohol concentration and salt concentration and high temperature shock improve antibiotic productivity several times [Nakata,
K. Et al., Biosci. Biotechnol. Bio
chem. 63, 293-297 (1999)]. S2
The antibiotic production of 72 strains is based on the autoinducer substance N
It has also been shown that high autoinducer activity under stress, regulated by -acyl-homoseline lactone, leads to induction of antibiotic production. A patent application has already been filed for a plant growth promoter using a culture of the S272 strain and for use as a plant spoilage inhibitor [Japanese Patent Application No. 10-261046.
issue〕.

[0003] The present inventors have further studied the use of the above-mentioned culture of the S272 strain as a plant growth promoter and a plant spoilage inhibitor. As a result, it has been found that the excellent effects of various antibiotics such as pioluteolin, 2,4-diacetylphloroglucinol, and phenazinecarboxylic acid contained in the culture are not always effectively used in nature.

[0004] In the natural world, cell aggregation and adhesion reactions occur frequently. For example, microorganisms are devised to maintain their superiority in competition between organisms by forming colonies or parasitizing and symbiotic with the host. Attempts have been made to imitate such a natural mechanism and to adsorb and aggregate a plurality of types of cells by using an aggregating agent. For example, there is an example of the immobilized bacterial cell catalyst of Kokuda et al. [Biotechnol. Bioen
g. , 31, 382, (1988)]. However, flocculants often have an adverse effect on plants.

[0005] In nature, there are many symbiotic cases that do not form a special structure such as nodules in legumes. The strength of adhesion between heterologous organisms is determined by the affinity of each cell surface and the presence or absence of an adhesive substance, but is also affected by various environmental factors such as pH and ions. Artificial symbiosis control is an important issue in agriculture, as adhesion between heterogeneous organisms is expected to often favor both growths.

From the viewpoint of more effectively using the culture of the S272 strain as a plant growth promoter, the present inventors have paid attention to the utilization of symbiotic control for the purpose of artificial plant growth as described above. . However, as described above, the use of a coagulant known so far is not always advantageous for plants, and from the viewpoint of more effectively utilizing the culture of the S272 strain as a plant growth promoter, a new plant is used. It was necessary to develop symbiotic control for rearing.
In addition, there are many microorganisms having a characteristic ability in the natural world, but there have been very few studies on enhancing artificial regulation of the symbiotic relationship with plants. It is also known that polysaccharides produced by bacteria extracellularly often disrupt the plant defense system against pathogenic bacteria [Robe]
rts, Annu. Rev .. Microbiol. 5
0, 285-315 (1996)].

Accordingly, an object of the present invention is to provide a new drug useful for controlling symbiosis for plant growth. It is a further object of the present invention to provide a more effective plant growth promoter comprising, as a component, a culture of a microorganism belonging to the genus Pseudomonas including S272 strain.

[0008]

SUMMARY OF THE INVENTION The present invention relates to a plant growth promoter or a plant growth promoter containing a polysaccharide obtained from a culture of a microorganism belonging to the genus Klebsiella. The present invention further relates to Klebsiella (K
polysaccharides obtained from cultures of microorganisms belonging to the genus Lebsiella and Pseudomonas
s) A plant growth promoter comprising a culture of a microorganism belonging to the genus. In this specification, the plant growth promoter is
A substance that promotes the growth of plants by itself. A plant growth promotion aid cannot promote plant growth by itself, but enhances its effect when used in combination with other plant growth promoters. It means something to be done.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Klebsiel
The microorganism belonging to the genus la) is a facultative anaerobic bacterium, and is considered to be a strain having a nitrogen fixing ability under anaerobic conditions. Genetic improvement research has been energetically conducted. As microorganisms belonging to the genus Klebsiella,
For example, Klebsiella pneumoniae
ellapneumoniae) and Klebsiella oxytoca. Also, Klebs
For microorganisms belonging to the genus iella), there are already examples of research on growth promotion by spraying strains. However, there is no report of forming a special strong symbiotic structure with plants in nature.

In the present invention, Klebsiella (Klebsiella) is used.
A polysaccharide obtained from a culture of a microorganism belonging to the genus ella) is used as a plant growth promoter or a plant growth promoter. Microorganisms belonging to the genus Klebsiella are known to produce polysaccharides in their cultures. For example, Klebsiella pneumoniae (K
lebsiella pneumoniae) H12 produced polysaccharides at the 1998 meeting of the Society of Biotechnology, "K. pneumoniae H12 strain produced polysaccharide-based flocculant 1st report: production, isolation, activity study", and It has already been reported as "Component of Microbial Polysaccharide Flocculant (Polysaccharide Flocculant No. 2)". However, Klebsiella
It has not been known that components contained in cultures of microorganisms belonging to the genus can be used as plant growth promoters and plant growth promoters. In addition, K. pneumo
niae H12 strain was registered on September 1999 by the FERM P-
Has been deposited as

Cultivation of a microorganism belonging to the genus Klebsiella can be favorably carried out using a medium containing ethanol as a carbon source when producing a polysaccharide. There is no limitation on the nitrogen source, and for example, polypeptone, yeast extract, soybean powder and the like can be used. If it is not necessary to produce polysaccharides, Klebsiella (Kl
The carbon source and the nitrogen source used for culturing microorganisms belonging to the genus ebsiella) can be appropriately selected from those used for culturing microorganisms belonging to the genus Pseudomonas described below.

The culture conditions are Klebsiella (Kl)
It can be changed as appropriate according to the characteristics of the microorganism belonging to the genus ebsiella), external conditions, and the like. More specifically, Klebsiella pneum
oniae) In the case of the H12 strain, the pH of the medium is about 6 to 8. Usually, it is preferable to be near neutral. The culture temperature is a temperature at which the microorganism grows well. That is, the culture temperature is 15 ° C to 40 ° C, preferably 25 ° C.
~ 35 ° C. The culturing time depends on other culturing conditions, but is generally about 2 to 10 days, preferably 2 to 5 days.

[0013] Klebsiella pneumoniae (Kleb
siella pneumoniae and Klebsiella oxytoc
More specifically, a) is a medium containing ethanol as a carbon source (for example, 2% ethanol, 1% glycerin, 0.5% yeast extract, 0.5%
Polypeptone, 0.05% K ₂ HPO ₄ , 0.05% M
_{gSO 4 · 7H 2 O, 0.2} % NaCl, 0.2% Ca
CO ₃ ). As shown in Table 1 below, Klebsiella pneumoniae
lla pneumoniae) and Klebsiella oxytoca
Cannot substantially produce polysaccharides in a medium containing no ethanol, and cannot achieve the object of the present invention. Cultures of microorganisms belonging to the genus Klebsiella contain extracellularly produced polysaccharides. Klebsiella pneumoniae
As shown in Example 2, the composition of the constituent sugars of the polysaccharide contained in the culture of S. ella pneumoniae strain H12 is, for example, galactose (35.23%), glucose (16.29%), mannose (2.33). %), Galacturonic acid (6.89%), glucuronic acid (2.12)
%), Glucosamine + galactosamine (7.38%), and the average molecular weight of the polysaccharide determined by gel filtration exceeds at least 200,000.

A culture of a microorganism belonging to the genus Klebsiella cultured in a medium containing ethanol contains a polysaccharide as described above. A culture of a microorganism belonging to the genus Klebsiella containing a polysaccharide, for example, a microbial cell in a culture solution, a culture solution, and a processed product thereof are directly used as a plant growth promoter or a plant growth promoter. Can be. Further, the culture solution can be freeze-dried and stored. Microbial cells are
It can also be stored as dried cells by microwaves or the like. Even when a freeze-dried culture solution or a dried bacterial cell is prepared and suspended again in water, it can be used as a plant growth promoter or a plant growth promoter. Further, polysaccharides contained in a culture such as a culture solution can be recovered and purified, and the recovered and purified polysaccharide can be used as a plant growth promoter and a plant growth promotion aid. Although ion exchange chromatography and gel filtration chromatography are effective for the recovery and purification of polysaccharides, a high recovery and a high degree of purification can be obtained by precipitation by addition of a water-soluble organic solvent such as ethanol.

If necessary, other additives can be added to the plant growth promoting agent and the plant growth promoting aid comprising the cultured product or the recovered and purified polysaccharide as long as the effect of promoting plant growth is not reduced. Alternatively, the plant growth promoting agent and the plant growth promoting aid composed of the culture or the recovered and purified polysaccharide may be diluted several times before use. Kleb Sierra
A plant growth promoting agent and a plant growth promoting agent containing a polysaccharide obtained from a culture of a microorganism belonging to the genus siella) include, for example, in the case of hydroponics, the concentration of the polysaccharide is 1 to 100 pp.
m can be added.

The above Klebsiella (Klebsiell)
a) Bacteria are described in Japanese Bacteriological Magazine (50, p. 1023, '9).
According to the microbial safety standard according to 5), the level is set to 1, so that mass culture production under proper management is easily performed. In particular, Klebsiella oxytoka (Klebsiella o
xytoca) ATCC 13182 strain has many studies as nitrogen fixing bacteria, and its safety has been pointed out.

Polysaccharides contained in cultures of microorganisms belonging to the genus Klebsiella are C
It has the ability to aggregate microorganisms in the presence of cations such as a ²⁺ and Mg ²⁺ , and is also effective for attaching various microorganisms to plants. Therefore, the plant growth promoter and the plant growth promoter of the present invention comprising a culture of a microorganism belonging to the genus Klebsiella may have a coexistence of cations such as Ca ²⁺ and Mg ²⁺ when an aggregating effect is required. It is preferable to use them.

The Klebsiel of the present invention
la) A plant growth promoter comprising a polysaccharide obtained from a culture of a microorganism belonging to the genus and a culture of a microorganism belonging to the genus Pseudomonas is a polysaccharide obtained from a culture obtained from the culture of the microorganism belonging to the genus Klebsiella. Sugars and Pseudomonas (Pseud
omonas) mixture with a culture of a microorganism belonging to the genus Omonas. A culture of a microorganism belonging to the genus Pseudomonas will be described below. Examples of microorganisms belonging to the genus Pseudomonas include, for example, Pseudomonas fluorescens.
Can be used. Preferred microorganisms belonging to the genus Pseudomonas include piorteoline, 2,4-diacetylphloroglucinol and N-
Pseudomonas fluorescens capable of producing (3-oxododecanoyl) -L-homoserine lactone
And particularly preferred examples include Pseudomonas fluorescens S272 strain. Pseudomonas fluorescens
The S272 strain was transferred to FERM P-1696 on August 26, 1998 by the Ministry of International Trade and Industry, National Institute of Advanced Industrial Science and Technology.
Deposited as No. 2.

For culturing microorganisms belonging to the genus Pseudomonas, a general method for culturing microorganisms can be used.
Preferably, the culture is performed under aerobic conditions such as shaking culture by liquid culture and aeration and agitation culture. A microorganism belonging to the genus Pseudomonas is inoculated into a nutrient medium, and cultured aerobically to grow the cells. The culturing is performed under a stress load state as described later.

The medium that can be used for the culture is not particularly limited, and may be any medium containing a nutrient that can be used by microorganisms belonging to the genus Pseudomonas. Examples of such media include various synthetic media, semi-synthetic media, and natural media. A carbon source and a nitrogen source can be used for the medium composition. As the carbon source, for example, ethanol, glucose, sucrose, syrup, molasses, dextrin, starch, glycerol, animal oil, vegetable oil and the like can be used alone or in combination. As the nitrogen source, soybean flour, wheat germ, corn staple liquor, cottonseed meal, meat extract, peptone, yeast extract, urea, ammonium sulfate and the like can be used alone or in combination. If necessary, an inorganic acid salt such as sodium chloride, magnesium sulfate, calcium carbonate, phosphate, and metal salt can be added.

Culture conditions can be appropriately selected within a range in which the strain used can grow well and produce the above antibiotics and signal substances. The culture conditions can be appropriately changed according to the characteristics of the microorganism belonging to the genus Pseudomonas to be used, external conditions, and the like. Specifically, in the case of Pseudomonas fluorescens S272 strain, the pH of the medium is 6-8.
It is about. Usually, it is preferable to be near neutral. The culture temperature is a temperature at which the microorganism grows well. That is, the culture temperature is 15 ° C to 30 ° C, preferably 25 ° C.
~ 28 ° C. The culturing time depends on other culturing conditions, but is generally about 2 to 10 days, preferably 3 to 5 days.

Pseudomonas fluorescens S27
The culturing of the two strains is preferably performed at least partly during the culturing period under a stress-loaded state. It is not necessary that at least a part of the culture be sufficient, and that the whole be performed under stress. It can be said that stress loading is to load an organism with a stress factor that suppresses growth. Examples of the stress factor include addition of a high-concentration water-soluble organic solvent, salts, saccharides, and amino acids, as well as heating and freezing. Examples of the aqueous organic solvent include alcohols such as methanol, ethanol, propanol and isopropanol, ketones such as acetone, and esters such as ethyl acetate. Salts include sodium chloride, potassium chloride,
Calcium chloride, ammonium chloride, sodium sulfate,
Potassium sulfate, ammonium sulfate and the like can be mentioned. When the stress factor is caused by heating, freezing, or the like, it is preferable to rapidly change the culture temperature during the culture.

Under the above-mentioned culture conditions, the amount of accumulated antibiotics such as pioluteolin and 2,4-diacetylphloroglucinol and N- (3-oxododecanoyl) -L-homoserine lactone as a signal substance in the culture medium. Is stopped, the culture is stopped, and a culture solution can be obtained.

The plant growth promoter of the present invention comprises a culture obtained by culturing a microorganism belonging to the genus Pseudomonas by the above method, and a polysaccharide obtained from a culture of the microorganism belonging to the genus Klebsiella. Consists of a mixture. A culture obtained by culturing a microorganism belonging to the genus Pseudomonas may be used as a plant growth promoter as it is, but as in the case of a polysaccharide obtained from a culture of a microorganism belonging to the genus Klebsiella, The culture solution may be freeze-dried, or the microbial cells may be stored as dried cells by microwaves or the like, and the freeze-dried culture solution or the dried cells may be suspended in water and used as a plant growth promoter. . In addition, as a culture solution or the like of a microorganism belonging to the genus Pseudomonas, it is preferable to use one obtained using ethanol as a stress factor. This is because when sodium chloride is added as a stress factor, it is necessary to desalinate the culture solution, and when heat treatment is performed as a stress factor, it becomes difficult to cope with a large-scale culture.

The Klebsiel of the present invention
la) A polysaccharide obtained from a culture of a microorganism belonging to the genus and a plant growth promoter comprising a culture of a microorganism belonging to the genus Pseudomonas may be used after being diluted several times. Other additives can be added to such an extent that the promoting effect is not reduced.

[0026]

The present invention will be described in more detail with reference to the following examples. Example 1 K. Examination of fermentation production conditions of pneumoniae H12 strain The carbon source and nitrogen source for polysaccharide production were optimized. K.
pneumoniae H12 strain in 50 ml of MY medium (0.3% yeast extract, 0.3% malt extract, 0.5%
Shake culture overnight in a 500 ml flask containing polypeptone (1% glucose). 70 ml of production medium (3%
Carbon source, 1% nitrogen source, 0.05% K ₂ HPO ₄ , 0.0
_{5% MgSO 4 · 7H 2 O} , 0.2% NaCl, 0.2
% CaCO ₃ ) in a 500 ml flask with shaking for 3 days. The quantification of the polysaccharide was measured by the dry weight after the ethanol precipitation purification operation. That is, 30 ml of the culture solution is
Centrifugation was performed at 000 rpm for 15 minutes. 60 ml of ethanol was added to the supernatant and left at 4 ° C. overnight. 300 precipitates
It was obtained by centrifugation at 0 rpm for 15 minutes, quantified after suction drying. As a result, the H12 strain produced extracellular polysaccharide at a concentration of 3.0 g / l in an ethanol medium-specific manner (Table 1). Extracellular production was low with a single carbon source of glycerin or glucose. As a nitrogen source, polypeptone,
Both yeast extract and soy flour were good. When the culture temperature was examined, no polysaccharide was produced at 37 ° C. or higher.

[0027]

[Table 1]

Example 2 Fermentation Production and Purification pneumoniae H12 strain in 50 ml of MY
Medium (0.3% yeast extract, 0.3% malt extract, 0.
500ml containing 5% polypeptone, 1% glucose)
The cells were cultured in a flask with shaking overnight. 1% seed 3 L mini jar fermenter with 1.5 L production medium,
The cells were cultured at 28 ° C. for 3 days with aeration of 0.5 vvm and stirring with a propeller at 300 rpm. The culture solution was subjected to centrifugal filtration, and a 2-fold volume of ethanol was added to the supernatant. Centrifugal filtration, dissolve the precipitate in 1 L of deionized water,
The precipitate was again precipitated with twice the amount of ethanol. After suction drying,
3.57 g of a white powder were obtained. This degree of purification is sufficient for use as a plant growth promoter and a plant growth promoter. However, for the purpose of obtaining a higher purity, 10 g
/ L and re-dissolve in deionized water to 1000 ml
Sephadex G-100 was charged, and deionized water was allowed to flow to collect non-retained fractions. A precipitate was obtained by adding ethanol twice the amount of the eluate, and dried under reduced pressure to obtain a sample. The molecular weight was greater than Dextran (MW 200,000). Heating at 100 ° C. and sonication resulted in a water solubility of up to 5 g / l.

Example 3 Production of polysaccharides from other Klebsiella strains Each strain shown in Table 2 was treated with 50 ml of MY medium (0.3% yeast extract, 0.3% malt extract, 0.5% polypeptone, 1%).
% Glucose) in a 500 ml flask containing shaking overnight. 70 ml of production medium (2% ethanol, 1%
Glycerin, 0.5% yeast extract, 0.5% polypeptone, 0.05% K ₂ HPO ₄ , 0.05% MgSO _4.
The cells were shake-cultured for 3 days in a 500 ml flask containing 7H ₂ O, 0.5% NaCl, 0.2% CaCO ₃ ). As a result, the production of cohesive polysaccharides was oxytocaATC
Even with the C13182 strain, K.K. pneumoniae H1
As much as two strains were recognized.

[0030]

[Table 2]

Reference Example 1 fluorescens
Preparation of bacterial solution of S272 fluorescens S272 strain (FERM)
P-16962) in 50 ml of YSE medium (2% (V
/ V) ethanol, 0.3% yeast extract, 0.3% soy flour, 0.05% K ₂ HPO ₄ , 0.05% MgSO _4.
7H ₂ O, 0.2% NaCl, 0.2% CaCO ₃ ) in a 500 ml flask containing shake culture for 2 days. f
fluorescens S272 was obtained.

Example 4 fluorescens
Effect of S272 Bacterial Solution on Promotion of Kaiware Growth Absorbent cotton was spread on a 6-hole plate, moistened with 10 ml of water, and 10 seeds of Kaiware were seeded. After cultivation under light irradiation at 28 ° C., the amount of P. fluoresce
ns S272 culture solution (Reference Example 1) and K. pneumo
An appropriate amount of a 1% aqueous solution of a polysaccharide (Example 2) obtained from the culture solution of Niae H12 strain was added, and CaCO ₃ 1
0 mg was added in the form of a suspension. Further, the average elongation (mm) of the stem when cultivated for 3 days was measured. As a result, as shown in Table 3, when the S272 culture solution was not added,
There was no growth promotion by polysaccharides from pneumoniae strain H12 cultures. When the S272 bacterial solution is added, it alone has a growth promoting action. pneu
The growth was further enhanced by polysaccharides from the culture of Moniae H12. The addition amount of the S272 culture solution is 10 to 10
The range of 0 μl is good. pneumoniae H1
The amount of polysaccharide added from two strain cultures is 10 to 100 ppm
In the range, good results were obtained.

[0033]

[Table 3]

Example 5 fluorescens
Effect of S272 Bacterial Solution on Kaiware Salt Resistance A 6-well plate was covered with absorbent cotton, moistened with 10 ml of water, and 10 seeds of Kaiware were seeded. Under light irradiation at 28 ° C., the amount of P.P. fluorescens
S272 culture solution (Reference Example 1) and K. pneumoni
Polysaccharide obtained from aeH12 strain culture solution (Example 2)
By adding an appropriate amount of an aqueous solution of 1%, further CaCO ₃ 10
mg in the form of a suspension. The next day, salt was added to 2%, and the average elongation (m
m) was measured. As a result, as shown in Table 4, S27
Even when the two bacterial solutions were not added, the growth was promoted by adding the polysaccharide from the H12 strain culture. When the S272 bacterial solution is added, it alone has a growth promoting action.
Growth was further promoted by the addition of polysaccharide from the strain culture.
The addition amount of the S272 culture solution is preferably in the range of 10 to 100 μl, and the addition amount of the polysaccharide from the H12 strain culture was 10 to 10 μl.
Good results were obtained in the range of 0 ppm. in this way,
With the combined use of the S272 culture solution and the H12 strain polysaccharide, a growth rate of about 80% was obtained as compared with normal cultivation without salt,
Salt damage can be reduced.

[0035]

[Table 4]

Example 6 fluorescens
Effect of S272 Bacterial Solution on Aspergillus pot cultivation A four-month outdoor pot soil test was performed. The test was performed in 5 sections per section at 25 cm plant height. Every 5 days, a predetermined dilution of the S272 culture solution and 10 ml of the polysaccharide solution obtained from the H12 strain culture were sprayed. As a result, the bacterial solution had an effect on the plant height, and the effect was enhanced by the combined use of a polysaccharide solution obtained from the H12 strain culture at 50 ppm (FIG. 1, FIG. 1).
2). In addition, although not shown, effects were observed in all of the number of leaves, the number of branches, and the number of flowers.

[0037]

According to the present invention, Klebsiella (Kle)
The present invention can provide a novel agent comprising a polysaccharide obtained from a culture of a microorganism belonging to the genus bsiella) and useful for controlling symbiosis for plant growth. Further, according to the present invention, a more effective plant growth comprising, as components, a polysaccharide obtained from a culture of a microorganism belonging to the genus Klebsiella and a culture of a microorganism belonging to the genus Pseudomonas including strain S272. An accelerator can be provided. In particular, polysaccharides obtained from cultures of microorganisms belonging to the genus Klebsiella are useful for preventing the infection of pathogenic bacteria and spoilage bacteria in plants. fluorescens S2
By using 72 strains in combination, an excellent growth promoting effect can be obtained. In addition, Pseudomonas (Pseudomo
and culture of a microorganism belonging to nas) genus, giving a polysaccharide derived from a culture of a microorganism belonging to Klebsiella (Klebsiella) ^genus, Ca 2+, by coexistence with the cations such as ^{Mg 2+} pH 7 or more, of these Ingredients can be aggregated to strengthen the symbiotic state.

[Brief description of the drawings]

FIG. 1 is a graph showing the P.P. fluoresce
Test results of the effect of ns S272 bacterial solution on pot cultivation of Aspergillus niger.

FIG. 2 shows a graph of P. fluoresce
Test results of the effect of ns S272 bacterial solution on pot cultivation of Aspergillus niger.

Claims (8)

[Claims] 1. Klebsiella
A plant growth promoter or a plant growth promoter comprising a polysaccharide obtained from a culture of a microorganism belonging to the genus. 2. Klebsiella
The microorganism belonging to the genus is Klebsiella pneumoniae or Klebsiella oxytoca (Klebsiella).
The plant growth promoting agent or the plant growth promoting auxiliary according to claim 1, which is a microorganism belonging to oxytoca). 3. Klebsiella
The microorganism belonging to the genus is Klebsiella pneumoniae H1
2 strains (FERMP-) or Klebsiella
Oxytoka (Klebsiella oxytoca)
The plant growth promoting agent or plant growth promoting agent according to claim 1 or 2, which is ATCC 13182 strain. 4. Klebsiella
A plant growth promoter comprising a polysaccharide obtained from a culture of a microorganism belonging to the genus and a culture of a microorganism belonging to the genus Pseudomonas. 5. Klebsiella
The microorganism belonging to the genus is Klebsiella pneumoniae or Klebsiella oxytoca (Klebsiella).
The plant growth promoter according to claim 4, which is a microorganism belonging to oxytoca). 6. Klebsiella
The microorganism belonging to the genus is Klebsiella pneumoniae H1
2 strains (FERMP-) or Klebsiella
Oxytoka (Klebsiella oxytoca)
The plant growth promoter according to claim 4 or 5, which is ATCC 13182 strain. 7. A pseudomonas (Pseudomona)
s) The microorganism belonging to the genus is Pseudomonas fluorescens.
The plant growth promoter according to any one of claims 4 to 6, which is a microorganism belonging to s). 8. Pseudomonas
s) The microorganism belonging to the genus is Pseudomonas fluorescein.
(ns) S272 strain (FERM P-16962). The plant growth promoter according to any one of claims 4 to 7.