JP2001095561A - New lactobacillus strain - Google Patents
New lactobacillus strainInfo
- Publication number
- JP2001095561A JP2001095561A JP27933299A JP27933299A JP2001095561A JP 2001095561 A JP2001095561 A JP 2001095561A JP 27933299 A JP27933299 A JP 27933299A JP 27933299 A JP27933299 A JP 27933299A JP 2001095561 A JP2001095561 A JP 2001095561A
- Authority
- JP
- Japan
- Prior art keywords
- strain
- lactobacillus
- bulgaricus
- atpase
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000186660 Lactobacillus Species 0.000 title claims abstract description 27
- 229940039696 lactobacillus Drugs 0.000 title claims abstract description 26
- 230000000694 effects Effects 0.000 claims abstract description 37
- 108091006112 ATPases Proteins 0.000 claims abstract description 36
- 102000057290 Adenosine Triphosphatases Human genes 0.000 claims abstract description 36
- 210000000170 cell membrane Anatomy 0.000 claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 abstract description 6
- 239000004310 lactic acid Substances 0.000 abstract description 6
- 235000014655 lactic acid Nutrition 0.000 abstract description 6
- 235000015140 cultured milk Nutrition 0.000 abstract description 3
- 230000002378 acidificating effect Effects 0.000 abstract description 2
- 235000021105 fermented cheese Nutrition 0.000 abstract description 2
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 description 12
- 230000004151 fermentation Effects 0.000 description 12
- 230000003247 decreasing effect Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 7
- 229930193140 Neomycin Natural products 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 229960004927 neomycin Drugs 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 235000014048 cultured milk product Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
- 241000831652 Salinivibrio sharmensis Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102220201851 rs143406017 Human genes 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- XUCIJNAGGSZNQT-JHSLDZJXSA-N (R)-amygdalin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O[C@@H](C#N)C=2C=CC=CC=2)O1 XUCIJNAGGSZNQT-JHSLDZJXSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- ZFIQGRISGKSVAG-UHFFFAOYSA-N 4-methylaminophenol Chemical compound CNC1=CC=C(O)C=C1 ZFIQGRISGKSVAG-UHFFFAOYSA-N 0.000 description 1
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 1
- 238000006037 Brook Silaketone rearrangement reaction Methods 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- 240000002605 Lactobacillus helveticus Species 0.000 description 1
- 235000013967 Lactobacillus helveticus Nutrition 0.000 description 1
- 241000194041 Lactococcus lactis subsp. lactis Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 235000014969 Streptococcus diacetilactis Nutrition 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 229940089837 amygdalin Drugs 0.000 description 1
- YZLOSXFCSIDECK-UHFFFAOYSA-N amygdalin Natural products OCC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC(C#N)c3ccccc3 YZLOSXFCSIDECK-UHFFFAOYSA-N 0.000 description 1
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 description 1
- 229940111685 dibasic potassium phosphate Drugs 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- YGHHWSRCTPQFFC-UHFFFAOYSA-N eucalyptosin A Natural products OC1C(O)C(O)C(CO)OC1OC1C(OC(C#N)C=2C=CC=CC=2)OC(CO)C(O)C1O YGHHWSRCTPQFFC-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000021001 fermented dairy product Nutrition 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 229940054346 lactobacillus helveticus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
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- 235000013372 meat Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- XONPDZSGENTBNJ-UHFFFAOYSA-N molecular hydrogen;sodium Chemical compound [Na].[H][H] XONPDZSGENTBNJ-UHFFFAOYSA-N 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
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- 235000019319 peptone Nutrition 0.000 description 1
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Landscapes
- Dairy Products (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、細胞膜結合性アデ
ノシントリホスファターゼ(ATPase)活性が低下している
ラクトバチルス・デルブルッキー・サブスピーシズ・ブ
ルガリクス(Lactobacillus delbrueckii subsp. bulg
aricus) 変異株に関する。TECHNICAL FIELD The present invention relates to a Lactobacillus delbrueckii subsp. Bulg, which has reduced cell membrane-bound adenosine triphosphatase (ATPase) activity.
aricus ) For mutants.
【０００２】[0002]
【従来の技術】ATPaseは、マグネシウムイオン又はカル
シウムイオンの存在下、アデノシン三リン酸のγ位のリ
ン酸を加水分解してアデノシン二リン酸と無機リン酸に
分解する酵素であるが、この加水分解反応の際に生成す
るエネルギーが生体にとって重要であることが知られて
いる。そして、乳酸菌のラクトバチルス・デルブルッキ
ー・サブスピーシズ・ブルガリクス(Lactobacillus de
lbrueckii subsp. bulgaricus) には、大腸菌等と同様
に細胞膜結合性ATPaseが存在することが知られている。
しかし、この細胞膜結合性ATPaseがラクトバチルス・デ
ルブルッキー・サブスピーシズ・ブルガリクス(Lactoba
cillus delbrueckii subsp. bulgaricus) の糖代謝や
エネルギー生産に及ぼす影響については解明されていな
い。さらに、この細胞膜結合性ATPase活性が低下してい
るラクトバチルス・デルブルッキー・サブスピーシズ・
ブルガリクス(Lactobacillus delbrueckii subsp. bu
lgaricus) の菌株は知られていない。2. Description of the Related Art ATPase is an enzyme which hydrolyzes a phosphate at the γ-position of adenosine triphosphate to give adenosine diphosphate and inorganic phosphate in the presence of magnesium ion or calcium ion. It is known that the energy generated during the decomposition reaction is important for living organisms. And lactic acid bacteria Lactobacillus del Brookes subspecies Bulgarix (Lactobacillus de
Lbrueckii subsp The. bulgaricus), it is known that the presence of cell membrane bound ATPase similar to the E. coli and the like.
However, this cell-membrane-associated ATPase is not compatible with Lactobacillus del Brooke subspecies Bulgarix (Lactobacillus).
cillus delbrueckii subsp. not been elucidated for the effects on glucose metabolism and energy production of bulgaricus). In addition, Lactobacillus delbruchy subspecies has reduced cell membrane-bound ATPase activity.
Bulgaricus (Lactobacillus delbrueckii subsp. Bu
lgaricus ) is not known.
【０００３】一方、大腸菌K-12株のピルビン酸生産菌よ
り形質導入によって細胞膜結合性ATPase欠失変異株を誘
導し、その発酵パターンを親株と比較したところ、細胞
膜結合性ATPase欠失変異株では菌体のエネルギーレベル
が低下するため解糖系が活性化し、菌体当たりの糖消費
及びピルビン酸生産能が顕著に増大することが知られて
いる。On the other hand, a cell membrane-binding ATPase-deficient mutant was induced by transduction from a pyruvate-producing strain of Escherichia coli K-12, and its fermentation pattern was compared with that of a parent strain. It is known that glycolysis is activated due to a decrease in the energy level of the cells, and the sugar consumption per cell and the pyruvate-producing ability are significantly increased.
【０００４】また、細胞膜結合性ATPase活性が低下して
いる乳酸桿菌ラクトバチルス・ヘルベチカス(Lactobaci
llus helveticus)菌株 (特許第 2824821号公報) 及び乳
酸球菌ラクトコッカス・ラクチス・サブスピーシズ・ラ
クチス(Lactococcus lactissubsp.lactis)菌株 (特開平
9-9954号公報) を選択して、それぞれの菌株を使用して
乳酸酸度の上昇を抑制した発酵乳製品が提案されてい
る。[0004] In addition, Lactobacillus helveticus ( Lactobacius lactis) having reduced cell membrane-associated ATPase activity.
Llus helveticus) strains (No. 2,824,821 discloses) and Lactococcus lactis ssp lactis (Lactococcus lactis subsp. lactis) strain (Japanese Patent Laid-Open
No. 9-9954), and a fermented milk product in which an increase in lactic acidity is suppressed by using the respective strains has been proposed.
【０００５】[0005]
【発明が解決しようとする課題】本発明者らは、ラクト
バチルス・デルブルッキー・サブスピーシズ・ブルガリ
クス(Lactobacillus delbrueckii subsp. bulgaricu
s) に関し、種々研究を進めていたところ、細胞膜結合
性ATPase活性が低下したラクトバチルス・デルブルッキ
ー・サブスピーシズ・ブルガリクス(Lactobacillus de
lbrueckii subsp.bulgaricus) を見出した。したがっ
て、本発明は、細胞膜結合性ATPase活性が低下している
ラクトバチルス・デルブルッキー・サブスピーシズ・ブ
ルガリクス(Lactobacillus delbrueckii subsp. bulg
aricus) 変異株を提供することを課題とする。本発明の
細胞膜結合性ATPase活性が低下しているラクトバチルス
・デルブルッキー・サブスピーシズ・ブルガリクス(Lac
tobacillus delbrueckii subsp. bulgaricus) 変異株
は、酸性側で急速に乳酸生成及び生育が低下するので、
発酵乳やチーズ等の発酵乳製品を製造する際に有用であ
る。SUMMARY OF THE INVENTION The present inventors have found that Lactobacillus delbrueckii subsp. Bulgaricu (Lactobacillus delbrueckii subsp.
s ), a variety of studies have been conducted, and Lactobacillus debruchy subspecies bulgaricus (Lactobacillus de
lbrueckii subsp. bulgaricus) was found. Accordingly, the present invention provides a Lactobacillus delbrueckii subsp. Bulgaricus (Lactobacillus delbrueckii subsp.
aricus ) To provide a mutant strain. The Lactobacillus delbruchy subspecies bulgaricus (Lac ) having reduced cell membrane-associated ATPase activity of the present invention
tobacillus delbrueckii subsp. bulgaricus) mutant, since rapid lactic acid production and growth in acidic decreases,
It is useful when producing fermented milk products such as fermented milk and cheese.
【０００６】[0006]
【課題を解決するするための手段】本発明の細胞膜結合
性ATPase活性が低下しているラクトバチルス・デルブル
ッキー・サブスピーシズ・ブルガリクス(Lactobacillus
delbrueckii subsp. bulgaricus) 変異株は、以下の
ようにして得ることができる。ラクトバチルス・デルブ
ルッキー・サブスピーシズ・ブルガリクス(Lactobacill
us delbrueckii subsp. bulgaricus) 親株からネオマ
イシン自然耐性株を取得し、このネオマイシン自然耐性
株の中、親株よりも生育が低下した生育低下株を得る。
そして、この生育低下株のATPase活性を測定し、親株の
ATPase活性と比較して10％以下にATPase活性が低下した
ATPase活性低下株を得る。Means for Solving the Problems Lactobacillus delbruchy subspecies bulgaricus (Lactobacillus ) having a reduced cell membrane-bound ATPase activity of the present invention.
delbrueckii subsp. bulgaricus) mutant strain can be obtained as follows. Lactobacillus del Brooke Subspecies Bulgarix (Lactobacill
us delbrueckii subsp. bulgaricus) Gets the neomycin natural resistant strains from the parent strain, among the neomycin natural resistant strains to obtain a growth reduction strain growth was lower than the parent strain.
Then, the ATPase activity of this growth-reduced strain was measured, and the parent strain was measured.
ATPase activity decreased to 10% or less compared to ATPase activity
A strain with reduced ATPase activity is obtained.
【０００７】このようにして得られたラクトバチルス・
デルブルッキー・サブスピーシズ・ブルガリクス(Lacto
bacillus delbrueckii subsp. bulgaricus) のATPase
活性低下株は、発酵過程においてpHが低下すると本来、
H+ を排出する細胞膜結合性ATPaseが低下しているた
め、 H+ がうまく排出されずに菌体内のpHが低下する。
そのため、その生育が抑制され、乳酸の生成量が低下す
るので、発酵後に乳酸の生成を抑制する必要がある発酵
乳製品等の製造に利用することが可能である。親株とAT
Pase活性低下株の発酵特性を比較すると、培養20時間ま
ではその生育に差は認められないが、その後ATPase活性
低下株の生育が低下した。培養36時間後には、ATPase活
性低下株の酸生成能は極端に低下し、培養液のpHは親株
4.06に対し4.52であった。[0007] The thus obtained Lactobacillus
Delbrooky Subspecies Bulgarix (Lacto
bulgaricus ATPase from Bacillus delbrueckii subsp.
When the pH decreases during the fermentation process,
Since the cell membrane-bound ATPase that excretes H + is decreased, H + is not excreted well, and the pH in the cells decreases.
Therefore, its growth is suppressed, and the amount of lactic acid produced is reduced, so that it can be used for the production of fermented milk products and the like that need to suppress the production of lactic acid after fermentation. Parent stock and AT
Comparing the fermentation characteristics of the Pase activity-reduced strains, there was no difference in the growth up to 20 hours of culture, but the growth of the ATPase activity-reduced strains decreased thereafter. After 36 hours of culture, the acid-producing ability of the strain with reduced ATPase activity is extremely reduced, and the pH of the culture solution is changed to
It was 4.52 compared to 4.06.
【０００８】以下に実施例を示し、本発明を詳しく説明
する。Hereinafter, the present invention will be described in detail with reference to Examples.
【実施例１】(1) ネオマイシン耐性株の取得 市販の発酵乳から分離したラクトバチルス・デルブルッ
キー・サブスピーシズ・ブルガリクス(Lactobacillus
delbrueckii subsp. bulgaricus) SBT 0164を親株とし
てネオマイシン自然耐性株を取得した。すなわち、親株
を市販のＭＲＳ液体培地を１／２希釈した培地（１／２
ＭＲＳ液体培地）３ml中に接種し、37℃で一晩培養した
後、得られた培養物を3,500rpm、10分、４℃で遠心集菌
後、0.85％塩化ナトリウム溶液で２回洗浄し、同溶液３
mlに懸濁した。その懸濁菌液を25〜50μg/ml濃度のネオ
マイシンを含む１／２ＭＲＳ液体培地寒天平板培地（１
／２ＭＲＳ液体培地に寒天 15g/lを添加した培地）に
0.1mlを塗抹し、37℃で3 日間嫌気培養した。そして、
生育したネオマイシン耐性株 108株を取得した。Example 1 (1) Acquisition of neomycin-resistant strain Lactobacillus delbruchy subsp. Bulgaricus (Lactobacillus isolated from commercially available fermented milk)
delbrueckii subsp. bulgaricus) has acquired the neomycin natural-resistant strains of the SBT 0164 as a parent strain. That is, the parent strain was prepared by diluting a commercially available MRS liquid medium by half (1/2).
After inoculating 3 ml of MRS liquid medium and culturing at 37 ° C. overnight, the resulting culture was centrifuged at 3,500 rpm for 10 minutes at 4 ° C., and washed twice with 0.85% sodium chloride solution. Solution 3
suspended in ml. The suspension was mixed with a 1/2 MRS liquid medium agar plate containing neomycin at a concentration of 25 to 50 µg / ml (1
/ 2 MRS liquid medium supplemented with agar 15g / l)
0.1 ml was spread and anaerobically cultured at 37 ° C. for 3 days. And
108 grown neomycin resistant strains were obtained.
【０００９】(2) 生育低下株の取得 上記のネオマイシン耐性株を１／２ＭＲＳ液体培地中で
振盪培養し、親株よりも生育が低下した生育低下株４株
を取得した。(2) Acquisition of growth-reduced strain The neomycin-resistant strain described above was shake-cultured in a 1/2 MRS liquid medium to obtain 4 growth-reduced strains whose growth was lower than that of the parent strain.
【００１０】(3) ATPase活性の測定 上記の生育低下株のATPase活性を測定した。なお、ATPa
se活性の測定は次のような方法により行った。まず、生
育低下株を１／２ＭＲＳ液体培地が３ml入ったスクリュ
ーキャップ付き試験管で16時間培養したものを１／２Ｍ
ＲＳ液体培地３mlの入ったスクリューキャップ付き試験
管に660 nmにおける吸光度が約0.03となるように接種
し、37℃で静置培養した。18時間培養して得られた定常
期の菌体を 8,000×g 、10分、4℃で遠心集菌後、同条
件で 2.5mM塩化マグネシウムを含む 100mMトリス−塩酸
緩衝液(pH 8)で２回洗浄した。その後、湿菌体1gを同緩
衝液５mlに懸濁して超音波処理し、４℃で遠心分離 (2
0,000×ｇ、10分間) して上清を回収した。そして、こ
の上清を４℃で超遠心分離(100,000×ｇ、１時間) して
沈澱を回収し、同緩衝液に懸濁して粗酵素液とした。粗
酵素液は氷冷して４℃で保存し、24時間以内に使用し
た。一方、 5.0mM塩化マグネシウムを含む50mMトリス−
塩酸緩衝液(pH 7)に基質として５mMアデノシントリリン
酸ナトリウムを溶解して基質溶液とした。(3) Measurement of ATPase activity The ATPase activity of the above-mentioned growth-reduced strain was measured. ATPa
The measurement of se activity was performed by the following method. First, the growth-reduced strain was cultured for 16 hours in a screw-capped test tube containing 3 ml of 1/2 MRS liquid medium, and the mixture was diluted to 1 / 2M.
A test tube with a screw cap containing 3 ml of RS liquid medium was inoculated so that the absorbance at 660 nm was about 0.03, and the mixture was allowed to stand at 37 ° C. and cultured. The cells in the stationary phase obtained by culturing for 18 hours are collected by centrifugation at 8,000 × g for 10 minutes at 4 ° C., and then centrifuged with 100 mM Tris-HCl buffer (pH 8) containing 2.5 mM magnesium chloride under the same conditions. Washed twice. Thereafter, 1 g of the wet cells was suspended in 5 ml of the same buffer, sonicated, and centrifuged at 4 ° C. (2
(0000 × g, 10 minutes). Then, the supernatant was ultracentrifuged (100,000 × g, 1 hour) at 4 ° C. to collect a precipitate, which was suspended in the same buffer to obtain a crude enzyme solution. The crude enzyme solution was cooled on ice and stored at 4 ° C. and used within 24 hours. On the other hand, 50 mM Tris containing 5.0 mM magnesium chloride
A substrate solution was prepared by dissolving 5 mM sodium adenosine triphosphate as a substrate in a hydrochloric acid buffer (pH 7).
【００１１】上記の基質溶液 500μl と粗酵素液 100μ
l を混合し、37℃で10分間反応させた後、氷冷した0.1N
塩酸 300μl を添加して反応を停止した。そして、表１
に示した組成の発色液 2.1mlを添加して18℃で10分間発
色させた後、3,000rpmで10分間遠心分離して沈澱を除去
し、 660nmにおける吸光度を測定した。標準物質をKH2P
O4とした標準曲線より遊離された無機リン酸をその吸光
度より算出した。酵素活性は１分間に遊離した無機リン
酸量で示した。比活性は、ウシ血清アルブミン（BSA)を
標準とし、Bio-Rad プロテイン・アッセイキットで測定
した蛋白質1mg あたりの酵素活性で示した(H.Kobayashi
and Anraku, J.Biochem, 71, 387-399(1972))。The above substrate solution (500 μl) and the crude enzyme solution (100 μl)
After mixing at 37 ° C for 10 minutes, ice-cooled 0.1N
The reaction was stopped by adding 300 μl of hydrochloric acid. And Table 1
Was added at 18 ° C. for 10 minutes, and the mixture was centrifuged at 3,000 rpm for 10 minutes to remove the precipitate, and the absorbance at 660 nm was measured. KH 2 P as reference material
The inorganic phosphoric acid released from the standard curve of O 4 was calculated from its absorbance. Enzyme activity was indicated by the amount of inorganic phosphoric acid released per minute. The specific activity was indicated by the enzyme activity per 1 mg of protein measured with a Bio-Rad protein assay kit using bovine serum albumin (BSA) as a standard (H. Kobayashi
and Anraku, J. Biochem, 71, 387-399 (1972)).
【００１２】[0012]
【表１】 ──────────────────────────────────── 5N硫酸 10ml 2.5％モリブデン酸アンモニウム 10ml ３％硫酸水素ナトリウム−１％パラメチルアミノフェノール硫酸 10ml 水 40ml ────────────────────────────────────[Table 1] 5N sulfuric acid 10ml 2.5% ammonium molybdate 10ml 3 % Sodium hydrogen sulfate-1% paramethylaminophenol sulfuric acid 10ml water 40ml ─────────────────────────────────── ─
【００１３】(4) 低ATPase活性株の取得 表２に示した通り、親株のATPase活性の10％以下のATPa
se活性を示した４株を取得した。なお、ATPase活性の最
も低い株は親株のATPase活性の７％であった。このATPa
se活性の最も低い株、ATPase活性低下株Ｂ株をラクトバ
チルス・デルブルッキー・サブスピーシズ・ブルガリク
ス(Lactobacillus delbrueckii subsp. bulgaricus)
SBT 10757 として、工業技術院生命工学工業技術研究所
に寄託した (受託番号:FERM P-17555)。(4) Acquisition of low ATPase-active strain As shown in Table 2, ATPa having 10% or less of the ATPase activity of the parent strain was obtained.
Four strains showing se activity were obtained. The strain with the lowest ATPase activity was 7% of the ATPase activity of the parent strain. This ATPa
The strain with the lowest se activity, the strain B with reduced ATPase activity, was used for the Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus delbrueckii subsp. bulgaricus ).
Deposited as SBT 10757 with the Institute of Biotechnology and Industrial Technology, National Institute of Advanced Industrial Science and Technology (Accession number: FERM P-17555).
【００１４】[0014]
【表２】 ──────────────────────────── 比活性(nmol/min/mg protein) ──────────────────────────── 親株 20.3 ATPase活性低下株Ａ 1.87 ATPase活性低下株Ｂ 1.44 ATPase活性低下株Ｃ 2.01 ATPase活性低下株Ｄ 1.78 ────────────────────────────[Table 2] 比 Specific activity (nmol / min / mg protein) ──────── ──────────────────── Parent strain 20.3 ATPase activity decreased strain A 1.87 ATPase activity decreased strain B 1.44 ATPase activity decreased strain C 2.01 ATPase activity decreased strain D 1.78 ──── ────────────────────────
【００１５】なお、このラクトバチルス・デルブルッキ
ー・サブスピーシズ・ブルガリクス(Lactobacillus de
lbrueckii subsp. bulgaricus) SBT 10757 は、親株の
ラクトバチルス・デルブルッキー・サブスピーシズ・ブ
ルガリクス(Lactobacillusdelbrueckii subsp. bulgar
icus) SBT 0164と同様、以下に示す性質を有していたの
で、ラクトバチルス・デルブルッキー・サブスピーシズ
・ブルガリクス(Lactobacillus delbrueckii subsp.
bulgaricus) と同定し得る。The Lactobacillus debruchy subspecies Bulgarix (Lactobacillus de
bulgaricus ) SBT 10757 is the parent strain of Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus delbrueckii subsp.
icus ) Like SBT 0164, it had the following properties, so Lactobacillus delbrucky subspecies Bulgarix (Lactobacillus delbrueckii subsp.
bulgaricus ).
【００１６】 Ａ 形態的性状 (1)細胞の形： 桿菌 (2)運動性： なし (3)胞子の有無： なし (4)グラム染色性： 陽性 Ｂ 培地上の生育状態 (1)培養温度15℃ 生育しない (2)培養温度45℃ 生育するA Morphological properties (1) Cell shape: Bacillus (2) Motility: None (3) Presence / absence of spores: None (4) Gram stainability: Positive B Growth on medium (1) Culture temperature 15 ℃ does not grow (2) Culture temperature 45 ℃ grows
【００１７】 Ｃ 生理学的性質 (1)カタラーゼ： 陰性 (2)グルコースよりガスを産生しない。 (3)グルコン酸よりガスを産生しない。 (4)グルコースよりホモ乳酸発酵によりＤ（−）乳酸を産生する。 (5)各種炭水化物の分解性 1. グルコース： ＋ 2. ラクトース： ＋ 3. フラクトース： ＋ 4. マンノース： − 5. ガラクトース： − 6. シュークロース： − 7. マルトース： − 8. セロビオース： − 9. トレハロース： − 10. メリビオース： − 11. ラフィノース： − 12. メレテトース： − 13. マンニトール： − 14. ソルビトール： − 15. ユースクリン： − 16. サリシン： − 17. アミグダリン： −C Physiological Properties (1) Catalase: Negative (2) Does not produce gas from glucose. (3) It does not produce gas from gluconic acid. (4) D (-) lactic acid is produced from glucose by homolactic fermentation. (5) Degradability of various carbohydrates 1. Glucose: + 2. Lactose: + 3. Fructose: + 4. Mannose: − 5. Galactose: − 6. Sucrose: − 7. Maltose: − 8. Cellobiose: − 9 Trehalose:-10. Melibiose:-11. Raffinose:-12. Meletetose:-13. Mannitol:-14. Sorbitol:-15. Euskulin:-16. Salicin:-17. Amygdalin:-
【００１８】[0018]
【試験例１】表３に示した培地を充填した 300ml容三角
フラスコに、親株であるラクトバチルス・デルブルッキ
ー・サブスピーシズ・ブルガリクス(Lactobacillus de
lbrueckii subsp. bulgaricus) SBT 0164とATPase活性
低下株としてラクトバチルス・デルブルッキー・サブス
ピーシズ・ブルガリクス(Lactobacillus delbrueckii
subsp. bulgaricus) SBT 10757 をそれぞれ接種し、培
養温度37℃で発酵特性を比較し、図１に示した。In 300ml Erlenmeyer flask filled with medium shown in Test Example 1] Table 3 is a parent strain Lactobacillus del Burukki ssp bulgaricus (Lactobacillus de
lbrueckii subsp. bulgaricus) SBT 0164 and Lactobacillus del Burukki ssp. bulgaricus as ATPase activity decreased strain (Lactobacillus delbrueckii
bulgaricus ) SBT 10757 was inoculated, and the fermentation characteristics were compared at a culture temperature of 37 ° C., and the results are shown in FIG.
【００１９】[0019]
【表３】 ──────────────────── ペプトン 5 (g/l) 肉エキス 2.5 酵母エキス 2.5 グルコース 10 第二リン酸カリウム 1 ツイーン80 0.5 クエン酸二アンモニウム 1 酢酸ナトリウム 2.5 硫酸マグネシウム 0.05 硫酸マンガン 0.025 ────────────────────[Table 3] ──────────────────── Peptone 5 (g / l) Meat extract 2.5 Yeast extract 2.5 Glucose 10 Dibasic potassium phosphate 1 Tween 80 0.5 Citric acid Diammonium 1 Sodium acetate 2.5 Magnesium sulfate 0.05 Manganese sulfate 0.025 ────────────────────
【００２０】発酵開始から発酵20時間までは親株及びAT
Pase活性低下株共に生育に差は認められないが、それ以
降はATPase活性低下株の方が約20％ほど生育が低かっ
た。発酵36時間後のpHは親株が4.06、ATPase活性低下株
は4.52であり、ATPase活性低下株の酸生成能は親株に比
べて顕著に低下していた。From the start of fermentation to fermentation for 20 hours, the parent strain and AT
No difference was observed in the growth of the Pase activity-reduced strains, but the growth of the ATPase activity-reduced strain was about 20% lower thereafter. The pH after 36 hours of fermentation was 4.06 for the parent strain and 4.52 for the ATPase-active strain, and the acid-producing ability of the ATPase-active strain was significantly lower than that of the parent strain.
【００２１】[0021]
【発明の効果】本発明のラクトバチルス・デルブルッキ
ー・サブスピーシズ・ブルガリクス(Lactobacillus de
lbrueckii subsp. bulgaricus) のATPase活性低下株
は、発酵過程においてpHが低下するとその生育が抑制さ
れるので、発酵後に乳酸の生成を抑制する必要がある発
酵乳製品等を製造する際に有用である。According to the present invention, Lactobacillus del Brookie subspecies Bulgarix (Lactobacillus de
lbrueckii subsp. bulgaricus ATPase activity decrease strain), because its growth the pH is reduced in the fermentation process is suppressed, which is useful in the production of fermented dairy products is necessary to suppress the generation of lactic acid after fermentation .
図１親株とATPase活性低下株との発酵特性を比較したも
のである。FIG. 1 is a comparison of fermentation characteristics between a parent strain and a strain with reduced ATPase activity.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） Ｃ１２Ｒ 1:225） Ｃ１２Ｒ 1:225） (72)発明者 横田 篤 北海道札幌市豊平区里塚159−41 Ｆターム(参考） 4B001 AC31 BC14 EC01 4B065 AA30X AC10 AC20 BA24 CA42 ──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 7 Identification symbol FI Theme coat ゛ (Reference) C12R 1: 225) C12R 1: 225) (72) Inventor Atsushi Yokota 159-41F, Satozuka, Toyohira-ku, Sapporo, Hokkaido Terms (reference) 4B001 AC31 BC14 EC01 4B065 AA30X AC10 AC20 BA24 CA42
Claims (2)
ーゼ(ATPase)活性が低下しているラクトバチルス・デル
ブルッキー・サブスピーシズ・ブルガリクス(Lactobaci
llus delbrueckii subsp. bulgaricus) 変異株。1. Lactobacillus delbruchy subsp. Bulgaricus having reduced cell membrane-bound adenosine triphosphatase (ATPase) activity.
llus delbrueckii subsp. bulgaricus ) mutant.
スピーシズ・ブルガリクス(Lactobacillus delbruecki
i subsp. bulgaricus) SBT 10757 (FERM P-17555)であ
る請求項１記載の変異株。2. Lactobacillus delbruecki subsp. Bulgaricus (Lactobacillus delbruecki)
i subsp. bulgaricus) SBT 10757 (mutant strain of claim 1 wherein the FERM P-17555).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27933299A JP4526620B2 (en) | 1999-09-30 | 1999-09-30 | New lactic acid strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27933299A JP4526620B2 (en) | 1999-09-30 | 1999-09-30 | New lactic acid strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001095561A true JP2001095561A (en) | 2001-04-10 |
| JP4526620B2 JP4526620B2 (en) | 2010-08-18 |
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| JP27933299A Expired - Fee Related JP4526620B2 (en) | 1999-09-30 | 1999-09-30 | New lactic acid strain |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5543345B2 (en) * | 2008-06-30 | 2014-07-09 | 株式会社明治 | Hard or semi-hard natural cheese and method for producing the same |
| WO2023038073A1 (en) | 2021-09-09 | 2023-03-16 | 株式会社明治 | Lactic acid bacteria, lactic acid bacteria starter, fermented milk, fermented milk production method, and screening method of lactic acid bacteria |
| WO2023038072A1 (en) | 2021-09-09 | 2023-03-16 | 株式会社明治 | Lactic acid bacterium, lactic acid bacterium starter, fermented milk, method for manufacturing fermented milk, and method for screening lactic acid bacterium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07123977A (en) * | 1993-11-04 | 1995-05-16 | Calpis Food Ind Co Ltd:The | Lactic bacteria and fermented dairy product |
| JPH099954A (en) * | 1995-07-04 | 1997-01-14 | Snow Brand Milk Prod Co Ltd | New lactic acid bacterium strain |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07123977A (en) * | 1993-11-04 | 1995-05-16 | Calpis Food Ind Co Ltd:The | Lactic bacteria and fermented dairy product |
| JP2824821B2 (en) * | 1993-11-04 | 1998-11-18 | カルピス株式会社 | Lactic acid bacteria and fermented milk products |
| JPH099954A (en) * | 1995-07-04 | 1997-01-14 | Snow Brand Milk Prod Co Ltd | New lactic acid bacterium strain |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5543345B2 (en) * | 2008-06-30 | 2014-07-09 | 株式会社明治 | Hard or semi-hard natural cheese and method for producing the same |
| JP2014158499A (en) * | 2008-06-30 | 2014-09-04 | Meiji Co Ltd | Hard or semi-hard natural cheese, and manufacturing method thereof |
| WO2023038073A1 (en) | 2021-09-09 | 2023-03-16 | 株式会社明治 | Lactic acid bacteria, lactic acid bacteria starter, fermented milk, fermented milk production method, and screening method of lactic acid bacteria |
| WO2023038072A1 (en) | 2021-09-09 | 2023-03-16 | 株式会社明治 | Lactic acid bacterium, lactic acid bacterium starter, fermented milk, method for manufacturing fermented milk, and method for screening lactic acid bacterium |
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| JP4526620B2 (en) | 2010-08-18 |
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