JP2001039811A - Weed control composition containing drechslera monoceras and reduced amount of chemical herbicide, and weed control method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for controlling weeds which car curtail an applied quantity of a chemically synthesized agrochemical and can decrease load to the environment by using Drechslera monoceras together with an existing chemical herbicide in a mixed state. SOLUTION: This weed control composition comprises Drechslera monoceras and at least one kind selected from the group consisting of a compound having a common name of oxaziclomefone, a compound having a common name of indanofan, a compound having a common name of oxadiargyl and [3-(2-chloro-4- methylsulfonyl-benzoyl)-4-phenylthio]bicyclo-[3,2,1]oct-3-en-2-one.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a fungus, Drexrelella monoceras, which is a filamentous fungus having no herbicidal activity on crops and having selective herbicidal activity only on the genus Echinochloa sp. 1- (3,5-dichlorophenyl) -1-methylethyl] -2,3-dihydro-6-methyl-5-phenyl-4H-1,3-oxazine-4-
On, 2- [2- (3-chlorophenyl) -2,3-epoxypropyl] -2-ethylindane-1,3-dione, 3- [2,4-dichloro-5- (2-propynyloxy) phenyl ] -5- (1,1-dimethylethyl)-
1,3,4-oxadiazol-2 (3H) -one or [3- (2-chloro-4-methylsulfonyl-benzoyl) -4-phenylthio] bicyclo- [3,2,1]
The present invention relates to a weed controlling composition containing octo-3-en-2-one and a paddy weed controlling method in which each component is applied alone, simultaneously or before or after.

[0002]

2. Description of the Related Art In recent years, the influence of chemical pesticides on the human body and the natural environment has become a problem, and interest in biological pesticides has been increasing. In Japan, research on microbial herbicides for paddy field weeds and lawn weeds has been conducted. In particular, studies have been conducted on nobies, which are highly damaging weeds of rice, and as described in, for example, Japanese Patent Application Laid-Open No. Hei 10-179139, the plants show no pathogenicity to rice and herbicides against nobies. Drex Lera, a filamentous fungus that is effective
Paddy field weed control agents containing Monoceras.

On the other hand, 3- [1- (3,5-dichlorophenyl) -1-methylethyl] -2,3-dihydro-6
Methyl-5-phenyl-4H-1,3-oxazine-4
-One (hereinafter referred to as Compound A) is a herbicide known as oxadiclomefone in a general name and described in Japanese Patent Application No. 4-40108. 2- [2- (3-Chlorophenyl) -2,3-epoxypropyl] -2-ethylindane-1,3-dione (hereinafter referred to as compound B) is known as a general name indanophane. 3040
43. 3- [2,4-dichloro-5-
(2-propynyloxy) phenyl] -5- (1,1-
Dimethylethyl) -1,3,4-oxadiazole-2
(3H) -one (hereinafter referred to as compound C) is known by a general name oxadiagyl and is described in JP-A-48-08936. [3- (2-chloro-4-methylsulfonyl-benzoyl) -4-phenylthio] bicyclo-
[3,2,1] oct-3-en-2-one (hereinafter referred to as compound D) is a herbicide described in JP-A-6-25144.

[0004]

DISCLOSURE OF THE INVENTION The present invention provides a method for controlling weeds which reduces the load on the environment by reducing the application amount of the chemical herbicide by mixing Drexlera monoceras with an existing chemical herbicide. Is to do.

Accordingly, an object of the present invention is to provide a fungus, Drexlera monoceras, which exhibits no pathogenicity to useful plants such as paddy rice and has herbicidal effect to Nobies, and compounds A, B, and C. , A weed controlling composition comprising one or more compounds selected from the group consisting of compound D, and Dorexlera monoceras, and one or more compounds selected from the group consisting of compound A, compound B, compound C, and compound D alone. Another object of the present invention is to provide a method for controlling weeds, which is applied simultaneously or before and after.

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, have no pathogenicity on crops such as rice, wheat, soybean and so on, and have a selective herbicidal action on velvet. And the compound A, B, C, D and at least one compound selected from the group consisting of the compounds A, B, C, and D are used in combination or separately to exert a synergistic herbicidal effect on Nobies. In addition, the present inventors have found that it is possible to control broadleaf weeds other than nobies, and completed the present invention.

That is, the present invention is as follows. [1] The genus Echinochlo shows no pathogenicity to useful plants
a sp.) and 3- [1- (3,5-dichlorophenyl) -1-
Methylethyl] -2,3-dihydro-6-methyl-5
Phenyl-4H-1,3-oxazin-4-one, 2-
[2- (3-chlorophenyl) -2,3-epoxypropyl] -2-ethylindane-1,3-dione, 3-
[2,4-dichloro-5- (2-propynyloxy) phenyl] -5- (1,1-dimethylethyl) -1,3,3
4-oxadiazol-2 (3H) -one, [3- (2
-Chloro-4-methylsulfonyl-benzoyl) -4-
Phenylthio] bicyclo- [3,2,1] oct-3-
A weed controlling composition comprising at least one compound selected from the group consisting of en-2-ones. [2] Echinochlo without the pathogenicity of useful plants
a sp.) and 3- [1- (3,5-dichlorophenyl) -1-
Methylethyl] -2,3-dihydro-6-methyl-5
Phenyl-4H-1,3-oxazin-4-one, 2-
[2- (3-chlorophenyl) -2,3-epoxypropyl] -2-ethylindane-1,3-dione, 3-
[2,4-dichloro-5- (2-propynyloxy) phenyl] -5- (1,1-dimethylethyl) -1,3,3
4-oxadiazol-2 (3H) -one, or [3
-(2-chloro-4-methylsulfonyl-benzoyl)
And at least one compound selected from the group consisting of -4-phenylthio] bicyclo- [3,2,1] oct-3-en-2-one, applied alone, simultaneously or before or after. Weed control method.

[0008]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail. The Dorexrela monoceras according to the present invention is a phytopathogenic filamentous fungus that has no pathogenicity to useful crops such as rice and has a selective herbicidal effect only on the velvet. Here, useful plants refer to grains and vegetables such as rice, wheat, barley, soybean, and corn. The feature of the strain according to the present invention is that, in addition to showing a herbicidal effect on Nobies, a PCR product obtained by performing a PCR reaction on a chromosome with a DNA having the nucleotide sequence of SEQ ID NO: 1 as a primer Is between 1.5 kbp and 1.6 kbp. As a representative strain of Drexlera monoceras according to the present invention, MH-1901 (FERM BP-6)
091) and MH-2001 (FERM BP-60)
92). Under the above deposit number, these strains were transferred from August 29, 1997 to the Institute of Biotechnology, Industrial Technology Institute of the Ministry of International Trade and Industry of the Ministry of International Trade and Industry at 1-3-1 Higashi, Tsukuba City, Ibaraki Prefecture. Has been deposited under the Budapest Treaty on the International Recognition of Deposits.

[0009] The method of using the Drexrelella monoceras according to the present invention as a composition for controlling weeds can be carried out by a method using live cells obtained by culturing, a method using a filtrate obtained by culturing cells, or both. There are methods to use. As the viable cells, any of hyphae, conidia, or hyphae containing both of them can be used. Filamentous fungi belonging to the genus Drexlera are disclosed in JP-A-7-7978.
As disclosed in Japanese Patent Publication No. 4 (1994), a large-scale culture method has been established, and the composition can be industrially used as a composition for controlling weeds. The application rate of the Dorexrela monoceras according to the present invention varies depending on the form of preparation and the treatment method, but 1 × 10 ⁶ -1 × 10 ¹² conidium / are,
Preferably, it is 1 × 10 ⁸ -1 × 10 ¹⁰ pieces / R.

On the other hand, compounds A and B are known as herbicides for Nobies, and compounds C and D are widely effective herbicides for annual weeds. Is not always satisfactory.

In the weed controlling composition and the method for controlling weeds of the present invention, drexlera monoceras and at least one of the compounds A, B, C and D are used for the control of nobies. An unexpected synergistic effect is obtained, which makes it possible to reduce the application rates of chemical herbicides or Drexlera monoceras. Regarding its mechanism of action, the absorption and translocation of Compounds A, B, C and D are increased due to the damage of the plant tissue caused by the infection of Drexlera monoceras according to the present invention, and the compounds A, B and C are likewise increased. , D, it is presumed that infection and growth of Drexlera monoceras are facilitated in plant tissues that have been inhibited or damaged by D, and that the herbicidal action is enhanced.

The form of the composition for controlling weeds according to the present invention is a formulation that can be used for ordinary agricultural chemicals. Specific examples include, but are not limited to, wettable powders, flowables, powders, wettable powders, and granules.
Also, Drexlera Monoceras and Compound A,
Although B, C and D may be used as they are, generally, a durable Drexlera monoceras conidia and compounds A, B, C and D are mixed with an inert solid carrier or liquid carrier, and It is desirable to prepare and use it in a formulation. As the carrier, any solid or liquid carrier can be used as long as it is generally used in horticultural medicines and is biologically inert, and is not limited to a specific one. For example, as the solid carrier, mineral powder such as clay, talc, bentonite, calcium carbonate, diatomaceous earth, white carbon, soybean powder, vegetable powder such as starch, polyvinyl alcohol, and high molecular compounds such as polyalkylene glycol are exemplified. . Examples of the liquid carrier include various organic solvents such as decane and dodecane, vegetable oils, mineral oils, and water. In addition, stabilizers such as surfactants and binders, as well as antioxidants and pH adjusters can be used as necessary.

The composition for controlling weeds according to the present invention is used in rice fields before and after transplanting rice. It is preferably used from the day of transplantation to 14 days after transplantation, and more preferably from 3 days to 10 days after transplantation.

The method for applying the composition for controlling weeds of the present invention comprises the steps of:
Follow the usual method of applying paddy agricultural chemicals. The application rate is 100 g to 10 kg per 10 ares as a preparation.
It is preferably applied at 500 g to 3 kg per 10 ares.

The present invention includes a method for applying Drexlera monoceras and a herbicidal composition containing at least one compound of the compounds A, B, C and D individually, simultaneously or before and after. The application rate, formulation type, treatment time, etc. of the active ingredient in this case are the same as in the case of the weed controlling composition described above.

[0016]

EXAMPLES Example 1 Condensate 1 of Drexlera monoceras MH-1901, wettable powder (mixture)
A suspension containing × 10 ⁹ and 90 g of Toalite (manufactured by Toa Kasei Co., Ltd.) were mixed well, air-dried sufficiently at room temperature, and then crushed. 0.3 g of the finely pulverized compound A was added to the crushed product.
2% by weight, and Neoperex (made by Kao)
Triton X-100 was added and mixed at 2% by weight to obtain a wettable powder of Drexlera monoceras according to the present invention.

Example 2 Wettable powder (single agent) Conidia 1 of Drexlera monoceras MH-1901
A suspension containing × 10 ⁹ and 90 g of Toalite (manufactured by Toa Kasei Co., Ltd.) were mixed well, air-dried sufficiently at room temperature, and then crushed. To this crushed product, 2% by weight of Neoperex (manufactured by Kao) and 2% by weight of Triton X-100 were added and mixed to obtain a wettable powder of Drexlera monoceras according to the present invention.

Example 3 Dust (mixture) Conidia 1 of Drexlera monoceras MH-2001
A suspension containing × 10 ⁹ and 90 g of Toalite (manufactured by Toa Kasei Co., Ltd.) were mixed well, air-dried sufficiently at room temperature, and then crushed. The finely pulverized compound B was added to and mixed with the crushed product to a concentration of 1% by weight to obtain a powder of Drexlera monoceras according to the present invention.

Example 4 Dust (single agent) Conidia 1 of Drexlera monoceras MH-2001
90 g of Toalite (manufactured by Toa Kasei Co., Ltd.) was well mixed with the suspension containing × 10 ⁹ . The mixture was air-dried sufficiently at room temperature and then crushed to obtain a powder of Drexlera monoceras according to the present invention.

Example 5 Flowable agent (mixture) The concentrations in the flowable agent were 79% by weight of water, 10% by weight of Demol N (manufactured by Kao) and 1% by weight of compound C, respectively.
These mixtures were wet-milled and mixed to obtain a suspension containing 1 × 10 ⁹ conidia of Drexlera monoceras MH-1901 and xanthan gum so that the concentration in the flowable agent was 0.4% by weight. Was added and mixed to obtain a flowable agent for Drexlera monoceras according to the present invention.

Example 6 Flowable agent (single agent) A mixture of these was mixed so that the concentrations in the flowable agent were 79% by weight of water and 10% by weight of Demol N (manufactured by Kao), respectively, and the mixture was mixed with Drexlera monoceras. A suspension containing 1 × 10 ⁹ conidia of MH-1901 and xanthan gum are added and mixed so that the concentration in the flowable agent becomes 0.4% by weight, to obtain a flowable agent of Drexlera monoceras according to the present invention. Was.

Test Example 1 Herbicidal effect of the mixture on paddy field weeds (wettable powder) Nobie seeds were planted in paddy soil packed in a 1 / 5,000a pot and grown until the Nobie reached the 2.0 leaf stage. Was. A wettable powder containing compound A or B was prepared according to the method described in Example 1 above. A predetermined amount of the obtained wettable powder was dispersed in 100 times the amount of water and dropped on the water surface. Cultivation was carried out in a greenhouse at a water depth of 4 cm and daytime of 30 ° C and nighttime of 20 ° C. 30 days after the treatment, the dry weight of the remaining individuals was measured, and the results are shown in Table 1 (Table 1) as the control rate against weeds according to the following formula. From Tables 1 and 2, it is clear that the composition for controlling weeds comprising Drexlera monoceras and Compound A or Compound B according to the present invention acts synergistically on Nobies and exhibits a high control effect. became.

[0023]

[0024]

[Table 1]

[0025]

[Table 2]

Test Example 2 Herbicidal Effect of the Mixture on Paddy Field Weeds (Floable Agent) Nobie seeds were planted in paddy soil packed in a 1 / 5,000a pot and grown until the Nobie reached the 2.0 leaf stage. . A flowable containing Compound C or Compound D was prepared according to the method described in Example 5 above. A predetermined amount of the obtained flowable agent was dropped on the water surface. Cultivation was carried out in a greenhouse at a water depth of 4 cm and daytime of 30 ° C and nighttime of 20 ° C. The dry weight of the remaining individual was measured 30 days after the treatment, and the control rate against weeds was determined by the formula shown in Test Example 1.
The results are shown in the table (Table 2). From Tables 3 and 4, it was revealed that the weed controlling composition comprising Drexlera monoceras and Compound C according to the present invention act synergistically on Nobies and show a high controlling effect.

[0027]

[Table 3]

[0028]

[Table 4]

[0029]

As described above, the composition for controlling weeds comprising the Drexlera monoceras according to the present invention and at least one compound selected from the group consisting of compound A, compound B, compound C and compound D, and the method for controlling weeds of the present invention, It acts synergistically on the velvet in paddy fields, has a practically sufficient control effect, and contributes to reducing the drawback in conventional weed control using only chemically synthesized pesticides, that is, reducing the burden on the environment.

[0030]

[Sequence list] SEQ ID NO: 1 Sequence length: 10 Sequence type :: Nucleic acid Topology: Linear sequence: CCCAAGGTCC 10

Continuing from the front page (72) Inventor Kenichi Yamaguchi 1144 Togo, Mobara-shi, Chiba Mitsui Chemicals Co., Ltd. (72) Inventor Seiya Marika 1144 Togo, Togo, Mobara-shi, Chiba Mitsui Chemicals Co., Ltd. F-term (reference) 4H011 AB01 AB02 BA06 BB07 BB08 BB10 BB21 DA02 DA15 DC05 DD01

Claims (10)

[Claims] 1. The genus Echinacea, which does not show pathogenicity to useful plants
nochloa sp.) and 3- [1- (3,5-dichlorophenyl)
-1-methylethyl] -2,3-dihydro-6-methyl-5-phenyl-4H-1,3-oxazin-4-one, 2- [2- (3-chlorophenyl) -2,3-epoxypropyl 2-ethylindane-1,3-dione, 3- [2,4-dichloro-5- (2-propynyloxy) phenyl] -5- (1,1-dimethylethyl)-
1,3,4-oxadiazol-2 (3H) -one,
At least one compound selected from the group consisting of [3- (2-chloro-4-methylsulfonyl-benzoyl) -4-phenylthio] bicyclo- [3,2,1] oct-3-en-2-one A composition for controlling weeds. 2. A PCR product obtained by performing a PCR reaction using a DNA having the nucleotide sequence of SEQ ID NO: 1 in the Sequence Listing as a template, using the DNA of the Drexerella monoceras as a template. The weed controlling composition according to claim 1, wherein the composition is 1.5 kbp to 1.6 kbp. 3. The Drexlera Monoceras has MH-1
The weed controlling composition according to claim 2, which is 901 (FERMBP-6091). 4. Drexlera Monoceras has MH-2
The weed controlling composition according to claim 2, which is 001 (FERMBP-6092). 5. The weed controlling composition according to claim 1, wherein the weed is a paddy weed. 6. The genus Echinacea which does not show pathogenicity to useful plants
nochloa sp.) and 3- [1- (3,5-dichlorophenyl)
-1-methylethyl] -2,3-dihydro-6-methyl-5-phenyl-4H-1,3-oxazin-4-one, 2- [2- (3-chlorophenyl) -2,3-epoxypropyl 2-ethylindane-1,3-dione, 3- [2,4-dichloro-5- (2-propynyloxy) phenyl] -5- (1,1-dimethylethyl)-
1,3,4-oxadiazol-2 (3H) -one or [3- (2-chloro-4-methylsulfonyl-benzoyl) -4-phenylthio] bicyclo- [3,2
1) A method for controlling weeds, which comprises applying at least one compound selected from the group consisting of octo-3-en-2-ones independently, simultaneously or before or after. 7. A PCR product obtained by performing a PCR reaction with a DNA of the base sequence described in SEQ ID NO: 1 by using the DNA of the Drexrelella monoceras as a template and the DNA having the base sequence of SEQ ID NO: 1 in the Sequence Listing. The method for controlling weeds according to claim 5, wherein the kneading amount is 1.5 kbp to 1.6 kbp. 8. The system according to claim 1, wherein said Drexrella monoceras is MH-1.
901 (FERMBP-6091). 9. The Drexlera Monoceras has MH-2
001 (FERMBP-6092). 10. The method for controlling weeds according to claim 1, wherein the weeds are paddy weeds.