JP2000279853A - Method and apparatus for spray-supplying sample - Google Patents

Abstract

PROBLEM TO BE SOLVED: To lessen the amount of a sample to be used and lower the cost by releasing compressed air, which is produced by compressing a prescribed amount of air to a prescribed pressure, to a sample storage part, in which the sample is stored, by operating a control valve and spraying the sample through a tip end opening through a needle penetrating in the sample storage part. SOLUTION: An injection needle 15 is so installed as to penetrate the bottom wall of a sample reservoir 10, which is a sample storage means, and be projected and supported in the reservoir 10, and a three-way valve 20, which is a control valve, is installed between an air compressor 2 and the sample reservoir 10. A joining pipe 21 on the upstream side of the three-way valve 20 is joined to the tip end joining part of a cylinder 3 and a slight amount of a sample S is put in the sample reservoir 10 and the sample reservoir 10 is joined to a joining pipe 22 on the downstream side of the three-way valve 20. After that, a prescribed amount of air compressed to a prescribed pressure in the cylinder 3 is released to the sample reservoir 10 to instantaneously jet the sample S in the sample reservoir 10 out of the tip opening of the injection needle 15 through the needle 15.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method and an apparatus for spraying a powder, liquid or slurry sample.

[0002]

2. Description of the Related Art A small amount of a powder or liquid sample is sprayed, for example, in a drug transpulmonary absorption experiment in the case of administering a drug to the respiratory system, a model animal such as a rat using a spray supply device having a syringe structure. Some administer drugs to the trachea.

FIG. 8 shows this conventional spray supply device. Cylinder 02 and piston of spray supply device 01 with syringe structure
03 constitutes the air pushing means, and the tip connecting portion 02 of the cylinder 02
A is connected to a sample reservoir 04 as a sample storage means. The sample reservoir 04 has a cylindrical shape with a bottom and an injection needle 05 protrudes through the bottom wall.

[0004] In a state where the inner volume of the cylinder 02 is increased by pulling the piston 03, the sample reservoir 04 containing the sample S therein is connected to the distal end joint portion 02a of the cylinder 02.
Then, when the piston 03 is pushed in, pressure is applied to the sample S in the sample reservoir 04, and the sample S is sprayed from the distal end opening through the injection needle 05.

[0005] When the spray supply device 01 is used for a drug transpulmonary absorption experiment, it can be performed according to the method of Schanker et al. That is, a tube is connected to the injection needle 05 of the spray supply device 01, scissors are inserted into the neck of the rat to expose the trachea, and the tube is inserted into a cut in the trachea, and the piston 03 of the spray supply device 01 is inserted. Is pressed to push the formulation in the sample reservoir 04 into a tube and spray it into the trachea.

In the laser diffraction / scattering type particle size distribution measurement, a spray supply device is provided to disperse the powder for the measurement, but the conventional spray supply device is located downstream from the outer periphery toward the dispersion nozzle. Then, air was flowed in, and the powder was sucked by the generated negative pressure and sprayed from the dispersion nozzle.

[0007]

In the former drug transpulmonary absorption experiment, the piston 03 of the spray supply device 01 is pushed to gradually apply pressure to the sample S in the sample reservoir 03 and push it out to the injection needle 05. Since the sample S is sprayed from the beginning, the airflow velocity of the dispersed air is not stable, and in the case of powder samples, there are many sprays in which the compressed powder is gradually aggregated. Absorption peaks may be delayed due to poor arrival.

[0008] Further, it is difficult to make the conditions for operating the piston 03 constant, and the amount of air and the amount of the preparation to be sprayed in each trial is not stable, so that there may be a large difference between operators.

Further, in the latter spray supply device in the particle size distribution measurement, it takes time until the spray state (dispersion state) is stabilized after the powder is sucked and sprayed, so that a large amount of powder needs to be prepared. Also, contamination by the sample inside the apparatus is large. The amount of supplied air is also large, and additional equipment such as a compressor for continuously supplying air is required, which increases costs.

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and an object thereof is to provide a low-cost sample spray supply method capable of spraying a small amount of a sample in an always stable dispersion state with a small amount of aggregates. And a device.

[0011]

In order to achieve the above object, the present invention provides a method for controlling a control valve to open a compressed air obtained by compressing a fixed amount of air to a predetermined pressure into a sample storage section in which a sample is stored. A sample spray supply method was used in which a sample was sprayed from a tip end opening through a needle communicating with the sample storage unit.

By actuating the control valve, a predetermined amount of compressed air is released to the sample reservoir at a predetermined pressure, and the sample is sprayed from the needle tip in a short time, so that a small amount of the sample is sprayed in a dispersed state with less aggregates. be able to. Each trial can be performed under substantially the same conditions without individual differences among operators, and a stable amount of sample can be sprayed in a stable dispersion state at all times.

In the particle size distribution measurement, a small amount of a sample may be used, and contamination in the apparatus is small. Further, ancillary equipment such as a compressor is not required, and cost can be reduced.

According to a second aspect of the present invention, in the method of the first aspect, powder,
A liquid or slurry sample is stored in a predetermined amount, and the fixed air is compressed to a predetermined pressure while the compressed air side of the control valve is closed.

[0015] Compressed air obtained by compressing the fixed amount of air to a predetermined pressure,
Since the sample is opened to the sample storage section in which a predetermined amount of the sample is stored, trial can be always performed under a constant condition, and a stable amount of the sample can be sprayed in a stable dispersion state.

According to a third aspect of the present invention, there is provided a compression means for compressing a fixed amount of air to a predetermined pressure, a control valve connected to a downstream opening of the compression means, and a sample storage connected to a downstream side of the control valve. A cylinder, a needle protruding from the sample storage cylinder and having an internal communication and an open end, and operating the control valve to convert the air compressed by the compression means into a powder, liquid or slurry sample. A sample spray supply device that opens to the stored sample storage cylinder, sprays the sample through the needle through the tip of the needle, and sprays the sample.

With the control valve closed on the compression means side, the compression means compresses the fixed amount of air to a predetermined pressure, and operates the control valve to release the compressed air to the sample storage cylinder. Since the sample is sprayed from the opening, the sample is sprayed in a short time, and the airflow velocity at the time of spraying is set to the optimum constant speed, so that even a small amount of sample can be sprayed in a dispersed state with less aggregates.

Each trial can be performed under substantially the same conditions without individual differences among operators, and a stable amount of sample can be sprayed in a stable and dispersed state at all times. In a drug transpulmonary absorption experiment, a fixed amount of a preparation can be efficiently reached to the deep lung. In the particle size distribution measurement, a small amount of sample may be used, the contamination in the apparatus is small, and additional equipment such as a compressor is unnecessary, so that the cost can be reduced.

According to a fourth aspect of the present invention, in the sample spray supply device of the third aspect, the compression means has a combined structure of a cylinder and a piston, the control valve is a three-way valve, and the three-way valve is Operate the cylinder to communicate with the outside air, pull the piston to draw a certain amount of air into the cylinder, operate the three-way valve to seal the cylinder, push the piston and press the piston into the cylinder at a predetermined pressure. And operating the three-way valve to communicate the inside of the cylinder with the sample storage cylinder to release compressed air to the sample storage cylinder and spray the sample from the tip opening through the needle. And

It has a simple structure in which a three-way valve, which is a directional control valve, is interposed between the cylinder and the sample storage cylinder. It is lightweight and small and easy to handle. Since the compression at a predetermined pressure and the opening to the sample storage cylinder can be sequentially performed, the work can be performed efficiently. Therefore, it can be easily used for drug transpulmonary absorption experiments, particle size distribution measurement, and the like.

According to a fifth aspect of the present invention, in the sample spraying device according to the third or fourth aspect, a small diameter tube is connected to the needle, and the small diameter tube is covered with a large diameter tube through a gap. The large-diameter tube has a base end communicating with the outside air.

In a drug transpulmonary absorption experiment, when the tip of a large-diameter tube is inserted into the trachea of a rat, the large-diameter tube can communicate with the inside of the trachea and the outside air to ensure respiration of the rat. A small diameter tube within the diameter tube can guide the sample and spray it into the trachea.

According to a sixth aspect of the present invention, in the sample spraying and supplying apparatus according to the third or fourth aspect, a dispersion nozzle is connected to the needle.

In the particle size distribution measurement, a sample sprayed from a needle tip opening into a good dispersion state with a small amount of agglomerates is further dispersed in a dispersion state suitable for measurement by a dispersion nozzle. Can be guided to.

[0025]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment according to the present invention will be described with reference to FIGS. The spray supply device 1 according to the present embodiment has a syringe structure, and FIG.
As shown in FIG. 5, in addition to the air compressor 2 composed of the same cylinder 3 and piston 5 as the conventional one, the sample reservoir 10 as the sample storage means and the injection needle 15 penetrating through the bottom wall of the sample reservoir 10 and supported by projection, A three-way valve 20, which is a directional control valve, is interposed between the air compressor 2 and the sample reservoir 10.

The transparent cylinder 3 of the air compressor 2 has a reduced diameter connecting portion 3a projecting from the tip of the cylindrical main body, and has marks 4a and 4b for positioning the position of the piston 5 which slides in the cylindrical main body. It is attached to two predetermined locations on the cylindrical main body. The piston 5 is provided at the tip of the piston rod 6 and moves the piston 5 by operating the end of the piston rod 6 protruding from the cylinder 3.

The sample reservoir 10 has a slightly tapered bottomed conical shape, and its inner surface gradually decreases in diameter as it goes from the opening to the tip, and smoothly curves to form a bottom surface. The injection needle 15 is supported on the bottom wall through the base end, and the base end opening is open to the inside facing the inner surface of the bottom wall of the sample reservoir 10.

The shapes of the sample reservoir 10 and the injection needle 15 can be changed according to the shape, particle size, characteristics, etc. of the sample.

The three-way valve 20 includes a connecting pipe 21 extending linearly,
A three-way pipe 22 and a suction pipe 23 extending perpendicularly to the pipe 22 extend from a central rotary valve 24, and an operation lever 25 for operating the rotary valve 24 is protruded. The upstream connecting pipe 21 is fitted and connected to the distal end connecting portion 3a of the cylinder 3, and the downstream connecting pipe 22 is partially fitted and connected to the sample reservoir 10.

By rotating the operation lever 25, the rotary valve 24 is rotated to close the downstream connecting pipe 22 so that the upstream connecting pipe 21 communicates with the intake pipe 23. A state in which the side connection pipe 22 communicates with the intake pipe 23, and a state in which the intake pipe 23 is closed and the upstream connection pipe 21 communicates with the downstream connection pipe 22.
The state can be switched.

The spray supply device 1 has the above-described structure. The upstream connection pipe 21 of the three-way valve 20 is connected to the tip connection portion 3a of the cylinder 3, and the sample S is stored in the sample reservoir 10.
A small amount (for example, 5 mg) and add 3 sample reservoirs 10
The downstream side connection pipe 22 of the direction valve 20 is connected. As the sample S, a powder, liquid or slurry sample is used.

In this trial, 5 mg of a powder having a particle diameter of 0.12 mm or less was put into the sample reservoir 10 as the sample S, and the trial was performed. The procedure of using the spray supply device 1 assembled in such a manner that the sample S is put in the sample reservoir 10 will be described with reference to FIG.

FIG. 4 schematically shows the three-way valve 20. First, as shown in FIG. 4, the operation lever 25 of the three-way valve 20 is operated to close the sample reservoir 10 side so that the cylinder 3 side and the intake pipe 23 are in communication with each other. Then, the piston 5 is positioned at the mark 4b, and a fixed amount (for example, 1 ml) of air is sucked.

Next, the operation lever 25 is operated to close the cylinder 3 as shown in FIG. 4, and the piston rod 6 is pushed in until the piston 5 is positioned at the mark 4a to compress the air in the cylinder 3 to a predetermined pressure. .

In this state, the operating lever 25 is operated to
The cylinder 3 and the sample reservoir 10 are communicated by closing the intake pipe 23 side as shown in FIG. A predetermined amount of air compressed to a predetermined pressure in the cylinder 3 is opened to the sample reservoir 10,
The sample S in the sample reservoir 10 is instantaneously sprayed from the tip opening through the injection needle 15.

A predetermined amount of compressed air is released into the sample reservoir 10 at a predetermined pressure, and the sample S is sprayed in a short time. Can be sprayed in a dispersed state with a small amount of water. Although all the samples S in the sample reservoir 10 are not sprayed, a constant amount of the sample S is always sprayed because the spraying conditions are constant.

Each trial can be performed under substantially the same conditions without individual differences among workers, and a stable amount of the sample S can be sprayed in a stable and dispersed state. The amount of the sample to be used may be small, and the contamination in the apparatus is small. Further, ancillary equipment such as a compressor is not required, and cost can be reduced.

FIGS. 5 and 6 show an example in which the present spray supply device 1 is applied to a drug transpulmonary absorption experiment. Silicon tube
35 is fitted to the sample reservoir 10 so as to cover the injection needle 15, while the other end of the silicone tube 35 is fitted with the upstream connecting pipe 31 of the three-way valve 30, and the sample reservoir 10 is inserted through the silicone tube 35. And the three-way valve 30 are connected.

The three-way valve 30 keeps the upstream connection pipe 31, the downstream connection pipe 32, and the intake pipe 33 all in communication. Then, a small-diameter polyethylene tube 36 into which the injection needle 15 is fitted penetrates the downstream connection pipe 32, the rotary valve, and the upstream connection pipe 31 of the three-way valve 30, and is fitted to the injection needle 15. A large-diameter polyethylene tube 37 capable of covering the small-diameter polyethylene tube 36 with a gap is fitted to the downstream connection pipe 32 of the three-way valve 30.

The inside of the sample reservoir 10 is in communication with the injection needle 15 and the inside of the small-diameter polyethylene tube 36, and the sample S in the sample reservoir 10 is sprayed from the injection needle 15 and guided to the small-diameter polyethylene tube 36 so as to be guided to the polyethylene tube 36. Is ejected from the tip opening of

The large-diameter polyethylene tube 37 which covers the small-diameter polyethylene tube 36 with a gap therebetween is 3
It communicates with the intake pipe 33 of the direction valve 30. Therefore, even if the polyethylene tube 37 is inserted into the incision in the trachea of the rat in the state shown in FIG.
In addition, the trachea and the outside air communicate with each other via the intake pipe 33 of the three-way valve 30, and the breathing of the rat is ensured.

With the rat breathing secured, the operating lever 25 of the three-way valve 20 is operated to release the compressed air into the sample reservoir 10 as described above, and the sample S is introduced into the trachea from the small-diameter polyethylene tube 36. Inject.

Since the sample S is sprayed in a dispersed state with a small amount of aggregates, it can efficiently reach the deep lung. When the absorption of the sample S is viewed from the time change of the blood concentration, the absorption peak is early. The spray supply device 1 is small and lightweight and easy to handle.

Further, by interposing a three-way valve between the cylinder of the conventional constant-volume syringe and the sample reservoir, the present spray supply device can be configured, and the conventional constant-volume syringe can be used, thereby reducing the cost. Can be achieved.

Next, FIG. 7 shows an example in which the present spray supply device 1 is applied to a particle size distribution measurement by a laser diffraction / scattering method. A dispersion nozzle 50 having a tapered surface whose inner diameter is gradually enlarged as an inner peripheral surface is fixed to the support table 51, and a hole of the support table 51 communicating with the base end opening of the dispersion nozzle 50 penetrates behind, This support
The injection needle 15 of the spray supply device 1 is inserted into the hole 51 from behind, and the sample reservoir 10 is fitted and supported.

The distal end of the injection needle 15 is inserted into the base end opening of the dispersion nozzle 50. An exhaust pipe 55 is disposed in front of the dispersion nozzle 50 via a measurement space, and a dust collector (not shown) is disposed downstream of the exhaust pipe 55.

Therefore, the sample S sprayed from the injection needle 15 of the spray supply device 1 into a good dispersion state with less aggregates is
Further, the dispersion nozzle 50 is set in a dispersion state suitable for measurement, and guided to a measurement space for laser diffraction / scattering type particle size distribution measurement. The sample S arriving at the measurement space is irradiated with laser light, causing optical phenomena such as diffraction and scattering, and analyzing the diffraction / scattering data to measure the particle size distribution of the sample S.

Since the sample S can be always measured in a stable and dispersed state with a small amount of aggregates by the present spray supply device 1, stable particle size distribution measurement is possible. In addition, a large amount of sample is not required for performing accurate particle size distribution measurement as in the prior art, and a small amount of sample may be used, and contamination in the apparatus may be reduced.

[0049] Further, ancillary equipment such as a compressor is not required, and the cost can be reduced. Furthermore, the measurement time is short. When the spray from the needle has good dispersibility, the dispersing nozzle is not always necessary.

In the above embodiment, the spray supply device 1 has a syringe structure. However, the present invention is not limited to such a structure.
In particular, when the present invention is applied to particle size distribution measurement, any structure may be used as long as it can compress a predetermined amount of air to a predetermined pressure and instantaneously open the sample reservoir, and it can be automated.

If the air compression means is a combination of a cylinder and a piston, the sensor detects the position of the piston, inhales a predetermined amount of air, and controls the sensor to automatically compress the air to a predetermined pressure. Can be.

In the above embodiment, powder is used as a sample, but the spray supply device of the present invention can be applied to spraying a liquid or a slurry.

[Brief description of the drawings]

FIG. 1 is a side view of a spray supply device according to an embodiment of the present invention.

FIG. 2 is a plan view of the same.

FIG. 3 is an exploded side view of the present spray supply device.

FIG. 4 is an explanatory diagram showing a procedure for using the present spray supply device.

FIG. 5 is a side view showing an application example of the spray supply device.

FIG. 6 is an exploded side view of the application example.

FIG. 7 is a side view showing another application example of the spray supply device.

FIG. 8 is a side view of a conventional spray supply device.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Spray supply apparatus, 2 ... Air compressor, 3 ... Cylinder, 5
... piston, 6 ... piston rod, 10 ... sample reservoir, 15 ... injection needle, 20 ... 3-way valve, 21, 22 ... connecting pipe, 23 ... intake pipe, 24 ... rotary valve, 25 ... operating lever, 30 ... 3-way Valve, 31,
32 ... connecting pipe, 33 ... intake pipe, 35 ... silicon tube, 36,
37 ... polyethylene tube, 50 ... dispersion nozzle, 51 ... support base, 55 ... exhaust pipe.

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[Procedure amendment]

[Submission date] January 19, 2000 (2000.1.1)
9)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Claims

[Correction method] Change

[Correction contents]

[Claims]

2. A compression means for compressing a fixed amount of air to a predetermined pressure; a control valve connected to a downstream opening of the compression means; a sample storage cylinder connected to a downstream side of the control valve; A needle protruding from the storage cylinder and having an interior communicating with a needle having an open end, and actuating the control valve to store the air, compressed by the compression means, into a powder, liquid or slurry sample. A sample spraying and feeding device characterized in that the sample is sprayed through the needle and opened to the body through a tip opening of the needle.

3. The compression means has a combination structure of a cylinder and a piston, the control valve is a three-way valve, and the three-way valve is operated to communicate the inside of the cylinder with outside air to pull the piston. A constant amount of air is sucked into the cylinder, the three-way valve is operated to seal the cylinder, the piston is pressed to compress the air in the cylinder to a predetermined pressure, and the three-way valve is operated to operate the cylinder and the cylinder. 3. The sample spray supply apparatus according to claim 2 , wherein the sample storage cylinder is opened to release compressed air to the sample storage cylinder, and the sample is sprayed from the tip opening through the needle.

4. A connecting the small diameter tube to said needle cover the large-diameter tube through a gap the same diameter tubes, according to claim 2, the base end side of the university diameter tube, characterized in that in communication with the outside air Or the sample spray supply device according to claim 3 .

5. A sample plume supply apparatus according to claim 2 or claim 3, wherein the connecting the dispersion nozzle to the needle.

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0011

[Correction method] Change

[Correction contents]

[0011]

To achieve SUMMARY and effect for solving the above objects, the present invention is powder in advance the sample reservoir, the liquid
A predetermined amount of body or slurry sample is stored, and the control valve
With the air side closed, the fixed amount air is compressed to a predetermined pressure,
Open to sample plume supplied to spraying of the sample from the tip opening through a needle in communication with the sample reservoir compressed air compressed quantitative air to a predetermined pressure to the sample reservoir in which the sample is stored by the operation of the control valve Method.

[Procedure amendment 3]

[Document name to be amended] Statement

[Correction target item name] 0012

[Correction method] Change

[Correction contents]

By actuating the control valve, a predetermined amount of compressed air is released to the sample reservoir at a predetermined pressure, and the sample is sprayed from the needle tip in a short time, so that a small amount of the sample is sprayed in a dispersed state with less aggregates. be able to. Compressed air was compressed to a specified pressure
A predetermined amount of sample is stored in the compressed air by operating the control valve.
Since the sample is opened to the sample storage section, each trial can always be performed under a constant condition without any individual difference between operators, and a stable amount of the sample can be sprayed in a stable and dispersed state at all times.

[Procedure amendment 4]

[Document name to be amended] Statement

[Correction target item name] 0014

[Correction method] Deleted

[Procedure amendment 5]

[Document name to be amended] Statement

[Correction target item name] 0015

[Correction method] Deleted

[Procedure amendment 6]

[Document name to be amended] Statement

[Correction target item name] 0016

[Correction method] Change

[Correction contents]

According to a second aspect of the present invention, there is provided a compression means for compressing a fixed amount of air to a predetermined pressure, a control valve connected to a downstream opening of the compression means, and a sample storage connected to a downstream side of the control valve. A cylinder, a needle protruding from the sample storage cylinder and having an internal communication and an open end, and operating the control valve to convert the air compressed by the compression means into a powder, liquid or slurry sample. A sample spray supply device that opens to the stored sample storage cylinder, sprays the sample through the needle through the tip of the needle, and sprays the sample.

[Procedure amendment 7]

[Document name to be amended] Statement

[Correction target item name] 0019

[Correction method] Change

[Correction contents]

[0019] According to a third aspect of the invention, the sample spray supply apparatus according to claim 2, wherein said compression means is a combined structure of the cylinder and the piston, wherein the control valve is a three-way valve, the three-way valve Operate the cylinder to communicate with the outside air, pull the piston to draw a certain amount of air into the cylinder, operate the three-way valve to seal the cylinder, push the piston and press the piston into the cylinder at a predetermined pressure. And operating the three-way valve to communicate the inside of the cylinder with the sample storage cylinder to release compressed air to the sample storage cylinder and spray the sample from the tip opening through the needle. And

[Procedure amendment 8]

[Document name to be amended] Statement

[Correction target item name] 0021

[Correction method] Change

[Correction contents]

According to a fourth aspect of the present invention, in the sample spraying device according to the second or third aspect , a small-diameter tube is connected to the needle, and the small-diameter tube is covered by a large-diameter tube through a gap. The large-diameter tube has a base end communicating with the outside air.

[Procedure amendment 9]

[Document name to be amended] Statement

[Correction target item name] 0023

[Correction method] Change

[Correction contents]

According to a fifth aspect of the present invention, in the sample spray supply device according to the second or third aspect, a dispersion nozzle is connected to the needle.

Claims (6)

[Claims] 1. A control valve is operated to release compressed air obtained by compressing a fixed amount of air to a predetermined pressure into a sample storage section in which a sample is stored, and the sample is sprayed from a tip opening through a needle communicating with the sample storage section. A method for supplying a sample by spraying, comprising: 2. A method according to claim 1, wherein a predetermined amount of a powder, liquid or slurry sample is stored in the sample storage section in advance, and a fixed amount of air is compressed to a predetermined pressure while the compressed air side of the control valve is closed. 2. The method according to claim 1, wherein: 3. A compression means for compressing a fixed amount of air to a predetermined pressure; a control valve connected to a downstream opening of the compression means; a sample storage cylinder connected downstream of the control valve; A needle protruding from the storage cylinder and having an interior communicating with a needle having an open end, and actuating the control valve to store the air, compressed by the compression means, into a powder, liquid or slurry sample. A sample spraying and feeding device characterized in that the sample is sprayed through the needle and opened to the body through a tip opening of the needle. 4. The compression means is a combination structure of a cylinder and a piston, the control valve is a three-way valve, and the three-way valve is operated to communicate the inside of the cylinder with outside air to pull the piston. A constant amount of air is sucked into the cylinder, the three-way valve is operated to seal the cylinder, the piston is pressed to compress the air in the cylinder to a predetermined pressure, and the three-way valve is operated to operate the cylinder and the cylinder. 4. The sample spray supply device according to claim 3, wherein the sample storage cylinder is opened to release compressed air to the sample storage cylinder, and the sample is sprayed from the tip opening through the needle. 5. The small-diameter tube is connected to the needle, the small-diameter tube is covered by a large-diameter tube through a gap, and a proximal end of the large-diameter tube communicates with outside air. Alternatively, the sample spray supply device according to claim 4. 6. The sample spray supply device according to claim 3, wherein a dispersion nozzle is connected to the needle.