JP2000270786A - Composition for antioxidation feed - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a safe composition for a freshness-keeping feed effective for preventing the oxidation of meat of cultured fish such as yellowtail during storage without deteriorating the quality of meat product and useful for a feed for pisciculture by making the composition include a polyphenol compound, an emulsifier, vitamin E, etc. SOLUTION: The objective composition contains (A) at least one kind of polyphenol compound preferably selected from among (+)-catechin, (+)- gallocatechin, (-)-gallocatechin gallate, etc., (B) an emulsifier such as glycerol fatty acid ester, (C) vitamin E and (D) an oil and fat such as rapessed oil preferably in amounts of 1-20 wt.% for the component A, 4580 wt.% for the component B, 3-20 wt.% for the component C and 10-40 wt.% for the component D. Oxidation of fish meat during storage can be prevented by mixing the composition to a feed and supplying the feed to the cultured fish of breeding stage. The amount of the composition to be added to the feed is preferably 0.005-3.0 wt.%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a composition for an antioxidant feed for cultured fish.

[0002]

2. Description of the Related Art As a means for preventing the cultivated fish from being oxidized during storage, a method of freezing it at a distribution stage or keeping it at a low temperature by refrigeration has been used. Such a method using freezing or refrigeration has a problem that a large capital investment is required. In addition, when the cultured fish is frozen, the protein is frozen and denatured, which causes poor food.

Further, packaging materials used in the distribution stage have been developed for the purpose of preventing oxidation, but have the problem that the cost is high and the method is not simple.

Japanese Patent Publication No. 46-39058 discloses a freshness preserving agent for marine products obtained by treating marine products with catechin, and Japanese Patent Application Laid-Open No. 63-29153 discloses a tea leaf extract component, one or more types of tea leaf extract components. A method for preserving the quality of seafood, characterized by treating the seafood with a mixture of a polyhydroxy compound, one or more organic acids, and water,
JP-A-2-31641 discloses an extract of a camellia or camphoraceae plant and a freshness preserving agent for fish and shellfish containing an antioxidant, and JP-A-4-94640 discloses a vitamin C preservative.
An anti-fading agent for fish and animal foods and a method for preventing fading of fish and animal foods, each containing a tea extract and a vitamin E, are disclosed. However, these disclosed technologies do not
It is intended for processed foods such as salted goods. Also,
At present, the disclosed technology is prohibited by the current Food Sanitation Law from being used as raw foods such as fillets and sashimi or fresh fish after shipment or distribution.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to provide a composition for an antioxidant feed which is effective for preventing fish meat oxidation during storage of cultured fish and which does not impair the quality of the obtained product. And

[0006]

The gist of the present invention is [1].
A composition for an antioxidant feed for cultured fish, comprising a polyphenol compound, an emulsifier, vitamin E and oil and fat, and [2] the composition for an antioxidant feed for cultured fish according to [1], wherein the composition is 0.005 to 0.005. A feed for cultured fish, which contains 3.0% by weight.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention relates to a composition for an antioxidant feed for cultured fish containing a polyphenol compound, an emulsifier, vitamin E and oil and fat. By feeding and using it, an unprecedented effect of preventing the oxidation of fish meat without any treatment at the time of shipping and distribution is exhibited.

In the present invention, a polyphenol compound and vitamin E are used as substances having an antioxidant action. However, since the former is water-soluble, it is diffused into water when fed as it is, and spread to cultured fish. Inability to feed effectively. Further, since the latter vitamin E is fat-soluble, it cannot be uniformly mixed with the polyphenol compound in its original form. Therefore, the present invention has one major feature in that a water-soluble polyphenol compound and a fat-soluble vitamin E are formulated by further blending an emulsifier and an oil and fat, and the obtained antioxidant bait is An excellent effect of suppressing the diffusion of the components of the composition into water is exhibited. Thereby, the composition for antioxidant feed of the present invention can be more efficiently fed to cultured fish. Furthermore, by formulating the antioxidant feed composition of the present invention, it becomes possible to uniformly disperse the composition in the feed.

[0009] In the present specification, a cultured fish is a fish obtained by artificially rearing fish of the horse mackerel family (for example, yellowtail), Thailand, flounder, amberjack, gibberellfish, bonito, puffer fish and the like. The composition for an antioxidant feed for cultured fish of the present invention is preferably used effectively for fish of the horse mackerel family, particularly for yellowtail.

[0010] In the present specification, antioxidation is defined as at least one of, for example, suppressing increase of index values such as a methation rate, a TBA value and a POV value during storage of cultured fish.

The methemoglobin ratio refers to the oxidization ratio of myoglobin to metmyoglobin in the muscle tissue of the cultured fish.

[0012] The TBA value refers to the ratio of oxidation of free fatty acids in cultured fish.

[0013] The POV value refers to the oxidation ratio of fats and oils of cultured fish.

Specific methods for measuring the methation rate, TBA value and POV value are not particularly limited, but may be, for example, “Keeping of freshness of livestock products” [written by Kazuhisa Takasaka, published by Tsukuba Shobo Co., Ltd.] , (1991)] pp. 48-51 and 58.
To 59, and the like.

The composition for an antioxidant feed of the present invention contains a polyphenol compound, an emulsifier, vitamin E and fats and oils, and is used by mixing these with the feed and feeding the cultured fish at the rearing stage. Point to.

Examples of the polyphenol compound include, but are not particularly limited to, polyphenol compounds derived from plants such as camellia plants, grape seeds and sweet potatoes, polyphenol compounds derived from foods such as cocoa and red wine, and polyphenol compounds obtained by chemical synthesis. Is mentioned.

Examples of the plant-derived polyphenol compound include a plant as it is, an extract from the plant, an extraction residue, and the like.

The extract from the plant body may be a solvent-extracted fraction with water or an organic solvent such as alcohol, ethyl acetate, petroleum ether, a steam-distilled fraction, a compressed fraction, a fat-adsorbed fraction,
An extract obtained from a liquefied gas extraction fraction, a supercritical extraction fraction or a dry distillation fraction is exemplified. Above all, an extract obtained from a solvent extraction fraction is preferable, and an extract obtained by extraction using water and extraction using ethyl acetate is more preferable.

When extracted from a plant, the plant is preferably a Camellia plant from the viewpoint of the availability of raw materials and the content of the polyphenol compound, and tea (Camell) is preferred.
iasinensis L. Is particularly preferred. Among them, the polyphenol compound is preferably an extract of green tea, oolong tea or black tea, particularly preferably an extract of green tea.
The hot water extraction fraction is described in, for example, JP-A-8-103225.
Can be prepared by the method described in Japanese Patent Application Publication No.

Specific examples of the polyphenol compound used in the present invention include (+)-catechin, (+)-gallocatechin, (-)-gallocatechin gallate, (-)-epicatechin, and (-)-epicatechin. Gallate, (−)-epigallocatechin gallate, (−)-epigallocatechin gallate,
At least one selected from the group consisting of theaflavin monogallate A, theaflavin monogallate B and theaflavin digallate is exemplified.

The polyphenol compound may be used alone or
Mixtures of more than one species may be used. When used alone,
(-)-Epigallocatechin gallate, (-)-epicatechin gallate or (-)-gallocatechin gallate is preferred, and (-)-epigallocatechin gallate is more preferred.

The extract may be used as it is,
A purified product obtained by purification may be used. For purification, for example, an adsorption resin, a filtration membrane, or the like can be used.

For example, when tea is used, it is also possible to use a polyphenol compound obtained by extraction with ethyl acetate. More specifically, in the case of green tea, (+)-catechin, (+)-gallocatechin,
Polyphenol compounds of (−)-gallocatechin gallate, (−)-epicatechin, (−)-epicatechin gallate, (−)-epigallocatechin and (−)-epigallocatechin gallate are extracted.

The content of the polyphenol compound in the composition for an antioxidant feed is preferably 1 to 20% by weight, and 3 to 1% by weight.
5% by weight is more preferable, and 5 to 10% by weight is more preferable. The amount is preferably 1% by weight or more from the viewpoint of sufficiently exhibiting the antioxidant effect, and is preferably 20% by weight or less from the viewpoint of dispersibility in other feed components during the manufacture of the feed.

The emulsifier used in the present invention is preferably a food emulsifier approved by the Food Sanitation Law, for example, glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene Glycol fatty acid esters, lecithin and the like. From the viewpoint of safety and dispersibility in cultured fish, glycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester and lecithin are preferable, and such emulsifiers are used alone or in combination of two or more. be able to. In particular, among glycerin fatty acid esters, monoglycerin fatty acid esters and polyglycerin fatty acid esters are preferable. Specific examples include glycerin caprylate, glycerin caprate, glycerin laurate and condensed ricinoleic acid polyglycerol ester. Such emulsifiers may be used alone or in combination of two or more.

The content of the emulsifier in the composition for an antioxidant feed is preferably 45 to 80% by weight, more preferably 50 to 75% by weight, and still more preferably 55 to 70% by weight. From the viewpoint of suppressing the diffusion of the obtained composition in water, 45
% By weight or more, and preferably 80% by weight or less from the viewpoint of dispersibility with respect to other feed components during the manufacture of the feed.

Vitamin E used in the present invention includes d-α-tocopherol, d-γ-tocopherol, d-δ-tocopherol, etc. Such compounds may be used alone or in combination of two or more. Good.

The content of vitamin E in the composition for an antioxidant feed is preferably 3 to 20% by weight, and 5 to 1% by weight.
5% by weight is more preferable, and 7 to 12% by weight is more preferable. The content is preferably 3% by weight or more from the viewpoint of sufficiently exhibiting the antioxidant effect, and 20% by weight from the viewpoint of dispersibility in other feed components during the production of the feed.
The following is preferred.

The fats and oils used in the present invention are preferably edible fats and oils for feed, specifically, olive oil, cocoa butter, hardened oil, sesame oil, rice oil, safflower oil, shortening, refined tallow, Soybean oil, rapeseed oil, palm oil, palm kernel oil, margarine, cottonseed oil, coconut oil, peanut oil, lard, avocado oil, kapok oil, sardine oil,
Poppy oil, burdock oil, wheat malt germ oil, mackerel oil, shark oil,
Saury oil, cod oil, evening primrose oil, camellia oil, corn oil, babassu oil, sunflower oil, powdered fat, squid oil and the like. Such fats and oils may be used alone or in combination of two or more.

The content of fats and oils in the antioxidant feed composition is preferably 10 to 40% by weight, more preferably 15 to 35% by weight, and even more preferably 20 to 30% by weight. From the viewpoint of suppressing the diffusion of the obtained composition in water, 10
% By weight or more, and preferably 40% by weight or less from the viewpoint of dispersibility with respect to other feed components during feed production.

The content of the composition for an antioxidant feed in the feed is preferably 0.005 to 3.0% by weight, more preferably 0.05 to 3.0% by weight.
1.5% by weight is more preferred. From the viewpoint of sufficiently exhibiting the antioxidant effect, 0.005% by weight or more is preferable,
It is preferably at most 3.0% by weight.

The feeding period of the composition for an antioxidant feed of the present invention to cultured fish is not particularly limited, but the feeding period is preferably the whole feeding period, especially 14 to 30 days.

The timing of feeding the antioxidant feed composition of the present invention to cultured fish is not particularly limited, but feeding is preferably carried out until the date of landing. For example, when feeding for 14 to 30 days, it is preferable to feed from 14 to 30 days before the landing date.

For a cultured fish raised with a feed containing the antioxidant feed composition of the present invention, for example, when stored at 4 ° C. for 3 days in a state of three cuts, the methation ratio, TBA An excellent effect of suppressing an increase in the value and the POV value can be obtained. In addition, the obtained food is a safe food without quality loss.

[0035]

EXAMPLE 1 About 90 liters of water was added to 6 kg of green tea, heated with stirring, and extracted at 80 ° C. for 3 hours. The extract obtained by filtration was spray-dried to obtain 2.2 kg of a 25% pure polyphenol compound. After 10 liters of water was added to the mixture to dissolve it, the mixture was partitioned with ethyl acetate to obtain 0.8 kg of an ethyl acetate-soluble fraction.

The component composition of the obtained polyphenol compound is (+)-catechin 0.9% by weight, (+)-gallocatechin 9.9% by weight, (+)-gallocatechin gallate 4.
9% by weight, (-)-epicatechin 5.4% by weight, (-)
-Epicatechin gallate 7.5% by weight, (-)-epigallocatechin gallate 9.9% by weight and (-)-epigallocatechin gallate 25.8% by weight.

Example 2 0.4 kg of the polyphenol compound prepared in Example 1
(6% by weight in the obtained composition for antioxidant feed), 4.0 kg of glycerin fatty acid ester (61% by weight in the obtained composition for antioxidant feed), 0.6 kg of vitamin E (manufactured by Sankyo Co., Ltd., extracted vitamin) E emulsion "Sankyo"; 9% by weight in the obtained antioxidant feed composition) and rapeseed oil 1.5k
g (23% by weight in the obtained antioxidant feed composition) was mixed with a homomixer, and 6.5 kg of the antioxidant feed composition was mixed.
I got

Test Example 1 Using a yellowtail as a cultured fish, an effect test in the field of the composition for an antioxidant feed obtained in Example 2 was performed.
As a basic feed, a feed obtained by adding a mixed feed to minced fish meat normally used for raising yellowtail was used.

Section A was fed with only the basic feed, and Section A was fed with the diet prepared by adding the composition for antioxidant feed prepared in Example 2 to the basic feed at 0.09% by weight. Section B and the section fed with the feed prepared by adding the composition for antioxidant feed prepared in Example 2 to the basic feed at 0.9% by weight were designated as Section C.

The sea surface is divided into small pens (A to C) (3m x 3m).
, And 150 yellowtails with an average weight of 530 g at the start of breeding were bred in each cage.

Test Example 2 Yellowtail reared under the conditions of Test Example 1 for 14 days was carcased to prepare sashimi, and the sashimi was stored at 4 ° C. Then, on the first, third, and fifth days after storage, the sashimi methuring rate, TBA value, and POV value were measured, and compared with the values immediately after the carcass, respectively, to calculate the rate of increase. In addition, the conversion ratio, TBA value and POV
The values are based on the above-mentioned “Preservation of freshness of livestock products” on pages 48-51 and 5
It was measured by the method described on pages 8-59.

Table 1 shows the rate of increase in the methation rate after storage, Table 2 shows the rate of increase in the TBA value after storage, and Table 3 shows the rate of increase in the POV value after storage.

[0043]

[Table 1]

[0044]

[Table 2]

[0045]

[Table 3]

As shown in Tables 1 to 3, the sashimi of the sashimi in the sections B and C bred with the diet to which the composition for an antioxidant bait of the present invention was added was compared with the section A bred with the basic feed alone. The rate of increase in the methation rate, TBA value and POV value during storage was significantly suppressed.

Test Example 3 Yellowtail reared under the conditions of Test Example 1 for 30 days was carcased to prepare sashimi, and the sashimi was stored at 4 ° C. in a conventional refrigerator. Then, on the first, third, and fifth days after storage, the sashimi methuring rate, TBA value, and POV value were measured, and compared with those values immediately after the carcass, the rate of increase was calculated. Table 4 shows the increase rate of the methation rate after storage, Table 5 shows the increase rate of the TBA value after storage, and Table 6 shows the increase rate of the POV value after storage.

[0048]

[Table 4]

[0049]

[Table 5]

[0050]

[Table 6]

As shown in Tables 4 to 6, the sashimi of the sashimi was higher in the sections B and C fed with the diet to which the composition for an antioxidant feed of the present invention was added than in the section A fed with the basic feed alone. The rate of increase in the methation rate, TBA value and POV value during storage was significantly suppressed.

[0052]

EFFECT OF THE INVENTION The antioxidant feed composition for cultured fish of the present invention is characterized in that the fish meat during storage is prevented from being oxidized when the feed containing the composition is fed to the cultured fish and grown. It has excellent effects. Furthermore, the present invention is a new technology that focuses on the breeding management of farmed fish, unlike the conventional technology, can reduce capital investment, does not affect the quality of the final product, and has an antioxidant effect. It is much better than the conventional technology.

 ──────────────────────────────────────────────────続 き Continuing from the front page (72) Inventor Zhu Politics 9-5 Akabori Shinmachi, Yokkaichi-shi, Mie Taiyo Chemical Co., Ltd. (72) Inventor Reka Raju Geneja 9-5 Akabori Shinmachi, Yokkaichi-shi, Mie Prefecture Taiyo Kagaku Co., Ltd. F-term (reference) 2B005 GA01 LA06 LB06 MA04 MB07 2B150 AA08 AB20 DA08 DA54 DA55 DA57 DD31 DD44 DD57 DE15

Claims (4)

[Claims] 1. A composition for an antioxidant feed for cultured fish, comprising a polyphenol compound, an emulsifier, vitamin E and an oil or fat. 2. The polyphenol compound is (+)-catechin, (+)-gallocatechin, (−)-gallocatechin gallate, (−)-epicatechin, (−)-epicatechin gallate, (−)-epigallocatechin. The at least one member selected from the group consisting of, (-)-epigallocatechin gallate, theaflavin monogallate A, theaflavin monogallate B and theaflavin digallate.
The composition for an antioxidant feed according to the above. 3. A polyphenol compound of 1 to 20% by weight,
The composition for an antioxidant feed according to claim 1 or 2, comprising 45 to 80% by weight of an emulsifier, 3 to 20% by weight of vitamin E, and 10 to 40% by weight of a fat or oil. 4. A feed for cultured fish, comprising the antioxidant feed composition according to claim 1 in an amount of 0.005 to 3.0% by weight.