JP2000262497A - Dispensing appliance, dispensing system and dispensing method - Google Patents
Dispensing appliance, dispensing system and dispensing methodInfo
- Publication number
- JP2000262497A JP2000262497A JP11070466A JP7046699A JP2000262497A JP 2000262497 A JP2000262497 A JP 2000262497A JP 11070466 A JP11070466 A JP 11070466A JP 7046699 A JP7046699 A JP 7046699A JP 2000262497 A JP2000262497 A JP 2000262497A
- Authority
- JP
- Japan
- Prior art keywords
- test tube
- dispensing
- pressure
- needle
- liquid sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、ある試験管に収納
されている液状の試料の一部を他の試験管に分注するた
めの分注器具、分注システム及び分注方法に関し、例え
ば真空採血により採取された血液を分注するのに好適に
用いられる、分注器具、分注システム及び分注方法に関
する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a dispensing device, a dispensing system and a dispensing method for dispensing a part of a liquid sample contained in a certain test tube to another test tube. The present invention relates to a dispensing device, a dispensing system, and a dispensing method that are suitably used for dispensing blood collected by vacuum blood collection.
【０００２】[0002]
【従来の技術】従来、液状の試料を例えば試験管などに
採取した後、該液状の試料を分注するためにピペットな
どの実験器具や様々な分注装置が用いられている。2. Description of the Related Art Conventionally, laboratory instruments such as pipettes and various dispensing apparatuses have been used for dispensing a liquid sample after collecting the sample in, for example, a test tube.
【０００３】しかしながら、液状の試料によっては、ピ
ペットや通常の分注装置を用いることができないことが
ある。すなわち、人体もしくは環境などに悪影響を及ぼ
す液状の試料では、ピペットや通常の分注装置を用いる
ことができない。例えば、血液試料では、ウィルスなど
の微生物感染の可能性があるため、通常のピペットや分
注装置を用いた場合、取扱者が感染する恐れがある。[0003] However, depending on the liquid sample, it may not be possible to use a pipette or a normal dispensing device. That is, a pipette or a normal dispensing device cannot be used for a liquid sample that has a bad influence on the human body or the environment. For example, a blood sample may be infected with a microorganism such as a virus. Therefore, when a normal pipette or a dispensing device is used, a handler may be infected.
【０００４】また、例えば特願平１０−５０９５８６号
公報に記載の細胞機能測定の場合のように、採取した血
液を一定量のＬＰＳで刺激することを特徴とする方法で
は、ＬＰＳを遮断した状態で血液試料を分注する必要が
ある。すなわち、通常のピペットや分注装置を用いた場
合には、外気中のＬＰＳが混入し、正確な診断を行うこ
とができなくなる。In a method characterized by stimulating collected blood with a fixed amount of LPS, for example, as in the case of cell function measurement described in Japanese Patent Application No. 10-509586, the LPS is blocked. It is necessary to dispense the blood sample with. That is, when a normal pipette or a pipetting device is used, LPS in the outside air is mixed, and accurate diagnosis cannot be performed.
【０００５】従って、上記のように、人体もしくは環境
などに悪影響を及ぼす恐れがある液状試料や、外気との
接触により化学的もしくは物理的な変化を生じる恐れが
ある液状の試料などを取り扱う際には、液状の試料を外
部と接触させることなく分注する必要がある。従来、こ
の種の液状の試料を分注する場合、液状の試料が収納さ
れた閉鎖系容器から他の容器に注射器を用いて移しかえ
ることにより液状の試料の分注が行われていた。Accordingly, as described above, when handling a liquid sample which may have a bad influence on the human body or the environment, or a liquid sample which may cause a chemical or physical change due to contact with the outside air, etc. It is necessary to dispense a liquid sample without contacting the outside. Conventionally, when dispensing a liquid sample of this type, a liquid sample has been dispensed by transferring the liquid sample from a closed container containing the liquid sample to another container using a syringe.
【０００６】[0006]
【発明が解決しようとする課題】しかしながら、注射器
を用いる方法では、液状の試料の一部を他の容器に移動
させる操作が煩雑であり、かつ注射器の針先から試料が
たれたりし、感染などのおそれがあった。また、液状の
試料の一部を正確な量だけ他の容器に移動させることが
困難であった。However, in the method using a syringe, the operation of transferring a part of the liquid sample to another container is complicated, and the sample is dripped from the needle tip of the syringe, causing infection. There was a fear. Further, it has been difficult to move a part of the liquid sample to another container by an accurate amount.
【０００７】本発明の目的は、外気との接触を避けた状
態で液状の試料をある試験管から他の試験管に容易かつ
安全に、さらに高精度に移動させることを可能とする分
注器具、分注システム及び分注方法を提供することにあ
る。SUMMARY OF THE INVENTION An object of the present invention is to provide a dispensing device capable of moving a liquid sample from one test tube to another test tube easily, safely and with high precision while avoiding contact with the outside air. , A dispensing system and a dispensing method.
【０００８】[0008]
【課題を解決するための手段】本願の第１の発明は、液
状の試料が収納されており、内部が第１の圧力とされて
栓体により閉成されている第１の試験管から、第１の圧
力よりも低い第２の圧力とされて栓体により閉成されて
いる第２の試験管に、液状の試料の一部を第１の圧力と
第２の圧力との圧力差を利用して移動させる分注方法に
用いられる分注器具であって、両端に針先を有し、一端
の針先が第１の試験管の栓体に貫通され、他端の針先が
第２の試験管の栓体に貫通される針管と、前記針管の長
さ方向の途中に固定された保持部材とを備えることを特
徴とする。According to a first aspect of the present invention, there is provided a first test tube containing a liquid sample, the inside of which is set to a first pressure and closed by a stopper. A part of the liquid sample is subjected to a pressure difference between the first pressure and the second pressure in a second test tube which is closed at a second pressure which is set to a second pressure lower than the first pressure. A dispensing device used in a dispensing method of using and moving, having a needle tip at both ends, a needle tip at one end is penetrated by a stopper of the first test tube, and a needle tip at the other end is a second tip. A needle tube penetrated by the stopper of the test tube of No. 2 and a holding member fixed in the lengthwise direction of the needle tube are provided.
【０００９】また、第２の発明に係る分注システムは、
第１の発明に係る分注器具と、一端から第１の試験管を
挿入することを可能とするために該一端が開口されてお
り、他端に前記保持部材が係合される保持孔を有する筒
状ホルダーとを備えることを特徴とする。Further, the dispensing system according to the second invention comprises:
A dispensing device according to the first invention, and a holding hole in which one end is opened to enable insertion of the first test tube from one end, and the other end is engaged with the holding member. And a cylindrical holder having the same.
【００１０】本願の第３の発明に係る分注システムは、
第１の試験管の栓体に貫通される針先を一端側に有する
第１の針管と、第１の針管の他端側に固定された筒状の
第１の保持部材とを備える第１の分注器具と、第２の試
験管の栓体に貫通される針先を一端側に有する第２の針
管と、第２の針管の他端側に固定された筒状の第２の保
持部材とを備える第２の分注器具と、第１の分注器具の
第１の保持部材と、第２の分注器具の第２の保持部材と
を接続するコネクタとを備えることを特徴とする。[0010] The dispensing system according to the third invention of the present application comprises:
A first needle tube having at one end a needle tip that penetrates a plug of the first test tube, and a first cylindrical holding member fixed to the other end of the first needle tube. Dispensing device, a second needle tube having a needle tip penetrating through the stopper of the second test tube on one end side, and a cylindrical second holding fixed to the other end side of the second needle tube A second dispensing device including a member, a connector for connecting a first holding member of the first dispensing device, and a second holding member of the second dispensing device. I do.
【００１１】第３の発明に係る分注システムでは、好ま
しくは、上記コネクタが、第１，第２の針管の連通をオ
ン・オフし得る連通制御機構を有する。本願の第４の発
明は分注方法であり、液状の試料が収納されており、内
部が第１の圧力とされて栓体により閉成されている第１
の試験管と、内部が第１の圧力よりも低い第２の圧力と
されて栓体により閉成されており、第１，第２の圧力の
圧力差が、移動させる試料の量に応じて選ばれている第
２の試験管とを用意する工程と、第１の試験管と第２の
試験管とを連通し、第１の圧力と第２の圧力との圧力差
により液状試料の一部を第１の試験管から第２の試験管
に移動させる工程とを備えることを特徴とする。In the dispensing system according to the third invention, preferably, the connector has a communication control mechanism capable of turning on / off the communication between the first and second needle tubes. The fourth invention of the present application is a dispensing method, in which a liquid sample is stored, and a first pressure is applied to the inside of the liquid sample and closed by a stopper.
And the inside of the test tube is closed by a stopper with a second pressure lower than the first pressure, and the pressure difference between the first and second pressures depends on the amount of the sample to be moved. Preparing a selected second test tube, communicating the first test tube with the second test tube, and setting a liquid sample by a pressure difference between the first pressure and the second pressure. Moving the section from the first test tube to the second test tube.
【００１２】第４の発明に係る分注方法は、第１の発明
に係る分注器具及び第２，第３の発明に係る分注システ
ムを用いて行うことができる。第４の発明に係る分注方
法では、液状の試料として、血液試料を好適に用いるこ
とができる。The dispensing method according to the fourth invention can be performed using the dispensing device according to the first invention and the dispensing system according to the second and third inventions. In the dispensing method according to the fourth invention, a blood sample can be suitably used as the liquid sample.
【００１３】[0013]
【発明の実施の形態】以下、図面を参照しつつ本発明の
具体的な実施形態を説明する。図１は、本発明の第１の
実施例に係る分注システムの操作方法を説明するための
縦断面図であり、図２（ａ）及び（ｂ）は、それぞれ、
第１の実施例で用いられる筒状ホルダーと分注器具とを
示す図である。DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, specific embodiments of the present invention will be described with reference to the drawings. FIG. 1 is a longitudinal sectional view for explaining an operation method of a dispensing system according to a first embodiment of the present invention, and FIGS.
It is a figure showing a cylindrical holder and a pipetting instrument used in a 1st example.
【００１４】図２（ａ）に示す筒状のホルダー１は、真
空採血に際し用いられるホルダーと同様の構造を有す
る。すなわち、筒状ホルダー１は、内部に試験管を挿入
し得るように、一端に開口１ａを有する円筒状の形状を
有する。もっとも、筒状ホルダー１は、角灯状などの他
の筒状の形状であってもよい。The cylindrical holder 1 shown in FIG. 2A has the same structure as the holder used for vacuum blood collection. That is, the cylindrical holder 1 has a cylindrical shape having an opening 1a at one end so that a test tube can be inserted therein. However, the cylindrical holder 1 may have another cylindrical shape such as a square lamp shape.
【００１５】筒状ホルダー１は、全体が合成樹脂により
構成されているが、合成樹脂以外の金属などの他の材料
により構成されていてもよい。筒状ホルダー１の開口１
ａ側には、フランジ部１ｂが形成されている。フランジ
部１ｂは、筒状ホルダー１の操作に際しての操作性を高
めるため、並びに筒状ホルダー１をフランジ部１ｂ側を
下にして机上に容易に載置させるために設けられてい
る。The cylindrical holder 1 is entirely made of synthetic resin, but may be made of another material such as metal other than synthetic resin. Opening 1 of cylindrical holder 1
A flange portion 1b is formed on the side a. The flange portion 1b is provided for enhancing operability in operating the cylindrical holder 1 and for easily mounting the cylindrical holder 1 on a desk with the flange portion 1b side down.
【００１６】筒状ホルダー１の開口１ａが形成されてい
る側とは反対側の面には、端面１ｃが形成されている。
端面１ｃの中央に軸方向外側に突出した突出部１ｄが形
成されているもの、突出部１ｄには、保持孔１ｅが形成
されている。この保持孔１ｅの内面には雌ねじが形成さ
れている。An end surface 1c is formed on the surface of the cylindrical holder 1 opposite to the side where the opening 1a is formed.
A protrusion 1d protruding outward in the axial direction is formed at the center of the end face 1c, and a holding hole 1e is formed in the protrusion 1d. A female screw is formed on the inner surface of the holding hole 1e.
【００１７】他方、図２（ｂ）に示すように、分注器具
２は、両端に針先３ａ，３ｂを有する針管３を有する。
また、針管３の長さ方向の途中には保持部材４が固定さ
れている。保持部材４は、例えば合成樹脂や金属などに
より構成されており、筒状の形状を有し、針管３に外挿
されている。保持部材４の周面には、雄ねじ４ａが形成
されている。保持部材４は、筒状ホルダー１の保持孔１
ｅに挿入されるように構成されており、かつ雄ねじ４ａ
が保持孔１ｅの内面に形成された雌ねじに対してねじ込
まれるように構成されている。On the other hand, as shown in FIG. 2B, the dispensing device 2 has a needle tube 3 having needle tips 3a and 3b at both ends.
A holding member 4 is fixed in the lengthwise direction of the needle tube 3. The holding member 4 is made of, for example, a synthetic resin or metal, has a cylindrical shape, and is externally inserted into the needle tube 3. On the peripheral surface of the holding member 4, a male screw 4a is formed. The holding member 4 is provided with the holding hole 1 of the cylindrical holder 1.
e, and the external thread 4a
Is screwed into a female screw formed on the inner surface of the holding hole 1e.
【００１８】すなわち、雄ねじ４ａと保持孔１ｅの雌ね
じとにより、保持部４が保持孔１ｅに固定されるように
構成されている。もっとも、分注器具２を筒状ホルダー
１に固定するための構造については、上記雌ねじ及び雄
ねじ４ａを利用したものに限らず、保持部４を保持孔１
ｅに圧入する方法、保持部４を保持孔１ｅに着脱自在に
嵌合し得る適宜の構造などを採用することができ、特に
限定されるものではない。That is, the holding portion 4 is fixed to the holding hole 1e by the male screw 4a and the female screw of the holding hole 1e. However, the structure for fixing the dispensing device 2 to the cylindrical holder 1 is not limited to the structure using the female screw and the male screw 4a.
For example, a method of press-fitting into the holding hole 4e, an appropriate structure capable of detachably fitting the holding portion 4 to the holding hole 1e, and the like can be adopted, and are not particularly limited.
【００１９】上記筒状ホルダー１及び分注器具２を用い
て、第１の試験管から第２の試験管に外気との接触を遮
断した状態で液状の試料を分注する方法、図１、図３及
び図４を参照して説明する。A method of dispensing a liquid sample from the first test tube to the second test tube in a state in which the contact with the outside air is cut off by using the cylindrical holder 1 and the dispensing device 2, FIG. This will be described with reference to FIGS.
【００２０】まず、図３に示すように、液状の試料５が
収納された第１の試験管６を用意する。第１の試験管６
は、栓体７により閉成されている。この栓体７で閉成さ
れた第１の試験管６内は、第１の圧力とされている。こ
の第１の圧力については、後述する第２の圧力より高け
れば特に限定されないが、通常は、大気圧とされる。First, as shown in FIG. 3, a first test tube 6 containing a liquid sample 5 is prepared. First test tube 6
Is closed by the plug 7. The inside of the first test tube 6 closed by the stopper 7 is set to the first pressure. The first pressure is not particularly limited as long as it is higher than a second pressure described later, but is usually atmospheric pressure.
【００２１】また、液状の試料５を収納した第１の試験
管６を用意するにあたっては、その方法については特に
限定されない。すなわち、真空採血管などを利用し、第
１の試験管６に栓体７が取り付けられた状態で、血液な
どの液状の試料５を栓体７を貫通する採血針を用いて吸
引し、液状試料５を試験管６内に収納してもよい。ある
いは、第１の試験管６内に液状の試料５を収納した後、
栓体７により閉成してよい。In preparing the first test tube 6 containing the liquid sample 5, the method is not particularly limited. That is, a liquid sample 5 such as blood is sucked using a blood collection needle penetrating the plug 7 while the plug 7 is attached to the first test tube 6 using a vacuum blood collection tube or the like. The sample 5 may be stored in the test tube 6. Alternatively, after storing the liquid sample 5 in the first test tube 6,
It may be closed by the plug 7.
【００２２】もっとも、好ましくは、真空採血管のよう
に、真空吸引により液状の試料５を収納する方法が用い
られる。すなわち、第１の試験管６に栓体７が取り付け
られた構造に、真空吸引により液状の試料５を注入する
方法では、最初の液状試料５の収納操作を外気と遮断し
た状態で行うことができる。Most preferably, a method of storing a liquid sample 5 by vacuum suction, such as a vacuum blood collection tube, is used. That is, in the method in which the liquid sample 5 is injected into the structure in which the plug 7 is attached to the first test tube 6 by vacuum suction, the first storage operation of the liquid sample 5 is performed in a state in which it is shut off from the outside air. it can.
【００２３】次に、液状試料５が注入された第１の試験
管６に、図２（ａ）に示した筒状ホルダー１を取り付け
る。すなわち、筒状ホルダー１の開口１ａ側から第１の
試験管６を栓体７側から挿入する。さらに、分注器具２
を筒状ホルダー１に取り付ける。この場合、針先３ａ側
から分注器具２を保持孔１ｅ内に挿入していき、針先３
ａにより栓体７を貫通する。そして、保持部４の雄ねじ
４ａを保持孔１ｅの内周面の雌ねじとねじ合わせること
により、分注器具２が筒状ホルダー１に固定される。こ
の状態を図４に示す。Next, the cylindrical holder 1 shown in FIG. 2A is attached to the first test tube 6 into which the liquid sample 5 has been injected. That is, the first test tube 6 is inserted from the opening 1a side of the cylindrical holder 1 from the stopper 7 side. Furthermore, dispensing device 2
Is attached to the cylindrical holder 1. In this case, the dispensing instrument 2 is inserted into the holding hole 1e from the needle tip 3a side,
The plug 7 penetrates the plug 7 with a. Then, the dispensing device 2 is fixed to the cylindrical holder 1 by screwing the male screw 4a of the holding portion 4 with the female screw on the inner peripheral surface of the holding hole 1e. This state is shown in FIG.
【００２４】次に、図１に示すように、第２の試験管８
を用意する。第２の試験管８内の圧力は、第１の試験管
６内の圧力、すなわち第１の圧力より低い第２の圧力と
されて、栓体９により閉栓されている。この第２の圧力
の大きさは、第１の圧力と第２の圧力との圧力差を利用
して液状試料５の一部を移動させる量に応じて定められ
る。Next, as shown in FIG.
Prepare The pressure in the second test tube 8 is set to the pressure in the first test tube 6, that is, a second pressure lower than the first pressure, and the second test tube 8 is closed by the stopper 9. The magnitude of the second pressure is determined according to the amount by which a part of the liquid sample 5 is moved using the pressure difference between the first pressure and the second pressure.
【００２５】次に、図１に示すように、第２の試験管８
の上方において、筒状ホルダー１に固定された第１の試
験管６を上下転倒させ、分注器具２の針先３ｂを、栓体
９に突き刺し、貫通させる。その結果、第１の試験管６
と第２の試験管８とが分注器具２の針管３により連通さ
れる。その結果、第１の試験管６内の第１の圧力と、第
２の試験管８内の第２の圧力との圧力差により、液状試
料５が第２の試験管８内に流れ込む。この液状試料５の
移動量は、第１，第２の圧力の圧力差に依存することに
なる。従って、第２の圧力の大きさを、液状試料５を移
動させる量に応じて設定しておくことにより、所望量の
液状試料５を第１の試験管６から第２の試験管８に移動
させることができる。Next, as shown in FIG.
Above, the first test tube 6 fixed to the cylindrical holder 1 is turned upside down, and the needle tip 3b of the dispensing device 2 is pierced into the stopper 9 and penetrated. As a result, the first test tube 6
The second test tube 8 is communicated with the second test tube 8 by the needle tube 3 of the dispensing instrument 2. As a result, the liquid sample 5 flows into the second test tube 8 due to the pressure difference between the first pressure in the first test tube 6 and the second pressure in the second test tube 8. The moving amount of the liquid sample 5 depends on the pressure difference between the first and second pressures. Therefore, by setting the magnitude of the second pressure according to the amount by which the liquid sample 5 is moved, a desired amount of the liquid sample 5 is moved from the first test tube 6 to the second test tube 8. Can be done.
【００２６】しかも、上記分注操作に際しては、閉鎖系
容器である第１，第２の試験管６と分注器具２とを用い
るため、液状試料５が外気と接触することがない。よっ
て、本実施例の分注方法では、外気と遮断した状態で液
状試料５の分注を行い得るので、液状試料５として、感
染の恐れがある血液試料を用いた場合や、人体もしくは
環境などに悪影響を及ぼす毒物などを用いた場合であっ
ても、操作者に感染や汚染の恐れを引き起こすことなく
分注作業を安全に行うことができる。In addition, in the above dispensing operation, since the first and second test tubes 6 which are closed containers and the dispensing device 2 are used, the liquid sample 5 does not come into contact with the outside air. Therefore, in the dispensing method according to the present embodiment, the liquid sample 5 can be dispensed in a state where the liquid sample 5 is blocked from the outside air. Even when a toxic substance or the like that has an adverse effect on the operator is used, the dispensing operation can be performed safely without causing a risk of infection or contamination to the operator.
【００２７】第１，第２の試験管６，８を構成する材料
については特に限定されず、ガラス、合成樹脂などの通
常の採血管や試験管に用いる適宜の材料を用いることが
できる。また、栓体７，９についても、通常の採血管や
試験管を閉栓するために用いられるゴムやエラストマー
からなる栓体を用いることができる。The materials constituting the first and second test tubes 6 and 8 are not particularly limited, and appropriate materials used for ordinary blood collection tubes and test tubes, such as glass and synthetic resin, can be used. Further, as the plugs 7 and 9, plugs made of rubber or elastomer used for closing ordinary blood collection tubes or test tubes can be used.
【００２８】なお、針管３の長さについては、図１に示
した分注操作において、針先３ａが、液状試料５の液面
から最終的に露出することがないように選択することが
必要である。すなわち、液状試料５を第１の試験管６か
ら第２の試験管８に移動させた後においても、針管３の
針先３ａが液状試料５の液面よりも下方に位置するよう
に、針管３の保持部４から針先３ａまでの距離を選択す
ることが必要である。さもないと、針先３ａが液状試料
５の液面よりも上方に突出し、所望量の液状試料５を第
２の試験管８に移動させ得なくなる。It is necessary to select the length of the needle tube 3 so that the needle tip 3a is not finally exposed from the liquid surface of the liquid sample 5 in the dispensing operation shown in FIG. It is. That is, even after the liquid sample 5 is moved from the first test tube 6 to the second test tube 8, the needle tube 3 is positioned so that the needle tip 3 a of the needle tube 3 is located below the liquid surface of the liquid sample 5. It is necessary to select the distance from the holding portion 4 of the third to the needle tip 3a. Otherwise, the needle tip 3a protrudes above the liquid surface of the liquid sample 5, and it becomes impossible to move a desired amount of the liquid sample 5 to the second test tube 8.
【００２９】針管３を構成する材料についても、ステン
レスなどの適宜の金属を用いることができ、特に限定さ
れるものではない。図５は、本発明の第２の実施例に用
いられる分注器具を示す側面図である。分注器具１２
は、図２（ｂ）に示した分注器具２と同様に両端に針先
３ａ，３ｂを有する針管３を有する。分注器具１２が分
注器具２と異なる点は、保持部１４において、外周面の
両端に雄ねじ１４ａ，１４ｂが形成されていることにあ
る。The material constituting the needle tube 3 may be any suitable metal such as stainless steel, and is not particularly limited. FIG. 5 is a side view showing a dispensing device used in the second embodiment of the present invention. Dispensing device 12
Has a needle tube 3 having needle tips 3a and 3b at both ends, similarly to the dispensing instrument 2 shown in FIG. 2 (b). The dispensing device 12 differs from the dispensing device 2 in that male threads 14 a and 14 b are formed at both ends of the outer peripheral surface of the holding portion 14.
【００３０】すなわち、分注器具１２では、雄ねじ１４
ａ，１４ｂを利用して、保持部１４の長さ方向両側の何
れの側においても筒状ホルダーに固定することが可能と
れている。That is, in the dispensing device 12, the male screw 14
The holding portion 14 can be fixed to the cylindrical holder on both sides in the length direction of the holding portion 14 by using a and 14b.
【００３１】分注器具１２を用いた場合には、分注操作
に際し、第２の試験管８側においても筒状ホルダー１を
用いることができる。このような分注器具１２を用いた
分注操作を図６を参照して説明する。When the dispensing device 12 is used, the tubular holder 1 can be used also on the second test tube 8 side during the dispensing operation. A dispensing operation using such a dispensing device 12 will be described with reference to FIG.
【００３２】この分注方法では、第１の実施例の場合と
同様に、液状試料５が収納された第１の試験管６を筒状
ホルダー１に挿入し、分注器具１２を取り付け、上下逆
転させる。他方、図６に示されているように、内部が第
２の圧力とされた第２の試験管８についても、別の筒状
ホルダー１に挿入する。そして、第１の試験管６が挿入
された筒状ホルダー１を上下逆転させた状態で、分注器
具１２の針先３ｂを、別の筒状ホルダー１に挿入された
第２の試験管８側の栓体９に貫通させる。そして、分注
器具１２の雄ねじ１４ｂを第２の試験管８が挿入されて
いる側の筒状ホルダー１の保持孔１ｅにねじ込む。この
ようにして、２個の筒状ホルダー１を用いて、第１，第
２の試験管５，８を分注器具１２を介して連結すること
ができる。よって、分注操作に際し、鋭利な針先３ｂを
有する分注器具１２を筒状ホルダー１に挿入された第２
の試験管８側の栓体９に容易に貫通させることができる
とともに、貫通後には第２の試験管８が挿入された筒状
ホルダー１に分注器具１２が確実に固定される。よっ
て、分注操作をより安定にかつ安全に行うことができ
る。In this dispensing method, as in the first embodiment, the first test tube 6 containing the liquid sample 5 is inserted into the cylindrical holder 1, the dispensing device 12 is attached, and Reverse. On the other hand, as shown in FIG. 6, the second test tube 8 whose inside is set to the second pressure is also inserted into another cylindrical holder 1. Then, in a state where the cylindrical holder 1 in which the first test tube 6 is inserted is turned upside down, the needle tip 3b of the dispensing instrument 12 is inserted into the second test tube 8 inserted in another cylindrical holder 1. Through the plug 9 on the side. Then, the male screw 14b of the dispensing device 12 is screwed into the holding hole 1e of the cylindrical holder 1 on the side where the second test tube 8 is inserted. In this manner, the first and second test tubes 5 and 8 can be connected via the dispensing device 12 using the two cylindrical holders 1. Therefore, at the time of the dispensing operation, the dispensing device 12 having the sharp needle tip 3b is inserted into the cylindrical holder 1 in the second position.
Can be easily penetrated into the plug 9 on the side of the test tube 8, and after the penetration, the dispensing device 12 is securely fixed to the cylindrical holder 1 into which the second test tube 8 is inserted. Therefore, the dispensing operation can be performed more stably and safely.
【００３３】第１の実施例に係る分注システムでは、図
２（ａ）に示した筒状ホルダー１と、筒状ホルダー１と
は別部材としての分注器具２とが用いられたが、この筒
状ホルダー１及び分注器具２は、一体化されてもよい。
すなわち、図７に示すように、筒状ホルダー１に分注器
具２が完全に固定された分注システム２１を用いてもよ
い。分注システム２１では、分注器具２の保持部４の外
周面が保持孔１ｅに接着剤等を用いて固定されている。In the dispensing system according to the first embodiment, the cylindrical holder 1 shown in FIG. 2A and the dispensing device 2 as a separate member from the cylindrical holder 1 are used. The cylindrical holder 1 and the dispensing device 2 may be integrated.
That is, as shown in FIG. 7, a dispensing system 21 in which the dispensing device 2 is completely fixed to the cylindrical holder 1 may be used. In the dispensing system 21, the outer peripheral surface of the holding portion 4 of the dispensing instrument 2 is fixed to the holding hole 1e using an adhesive or the like.
【００３４】なお、分注システム２１においては、保持
孔１ｅ及び保持部材４をそれぞれ設ける必要は必ずしも
なく、筒状ホルダー１の突出部１ｄに針管３を固定する
ための針管３の外径とほぼ同等の内径を有する貫通孔を
形成し、該貫通孔に針管３を挿入し固定してもよい。In the dispensing system 21, it is not always necessary to provide the holding hole 1e and the holding member 4, respectively, and the outer diameter of the needle tube 3 for fixing the needle tube 3 to the protruding portion 1d of the cylindrical holder 1 is substantially equal to the outer diameter. A through hole having an equivalent inner diameter may be formed, and the needle tube 3 may be inserted and fixed into the through hole.
【００３５】図８（ａ）及び（ｂ）並びに図９（ａ）及
び（ｂ）は、本発明の第３の実施例に係る分注システム
を説明するための図である。第３の実施例の分注システ
ムでは、図８（ａ）及び（ｂ）に示す第１，第２の分注
器具３１，３２が用いられる。第１の分注器具３１は、
一端に針先３３ａを有する針管３３を有する。針管３３
の他端側には、第１の保持部材３４が固定されている。
第１の保持部材３４は、合成樹脂や金属などの材料から
なり、筒状の構造を有する。針管３３の他端は、第１の
保持部材３４の貫通孔に挿入され、かつ固定されてい
る。FIGS. 8A and 8B and FIGS. 9A and 9B are views for explaining a dispensing system according to a third embodiment of the present invention. In the dispensing system according to the third embodiment, first and second dispensing devices 31 and 32 shown in FIGS. 8A and 8B are used. The first dispensing device 31
A needle tube 33 having a needle tip 33a at one end is provided. Needle tube 33
A first holding member 34 is fixed to the other end of the first holding member 34.
The first holding member 34 is made of a material such as a synthetic resin or a metal, and has a cylindrical structure. The other end of the needle tube 33 is inserted into the through hole of the first holding member 34 and fixed.
【００３６】第２の分注器具３２についても同様に、一
端に針先３５ａを有する針管３５と、第１の保持部材３
４と同様に構成された第２の保持部材３６とを有する。
図９（ａ）及び（ｂ）は、第３の実施例で用いられるコ
ネクターを示す縦断面図及び斜視図である。コネクター
３７は、貫通孔３７ａを有する筒状部材である。コネク
ター３７の貫通孔３７ａは、両端が相対的に大きな径を
有するように構成されており、この相対的に径の大きな
大径部３７ａ1 ，３７ａ2 間に段差３７ｂ，３７ｂを介
して相対的に径の小さな小径部３７ｃを有する。大径部
３７ａ1，３７2 は、それぞれ、第１，第２の分注器具
３１，３２の保持部材３４，３６の端部が挿入されるよ
うに構成されている。なお、保持部材３４，３６の最大
径部分であるハブ３４ａ，３６ａに比べて、上記大径部
３７ａ1 ，３７ａ2 の径は小さくされている。Similarly, for the second dispensing device 32, the needle tube 35 having the needle tip 35a at one end and the first holding member 3
4 and a second holding member 36 configured in the same manner as in FIG.
FIGS. 9A and 9B are a longitudinal sectional view and a perspective view showing a connector used in the third embodiment. The connector 37 is a cylindrical member having a through hole 37a. Through hole 37a of the connector 37, both ends are configured to have a relatively large diameter, relative via step 37b, and 37b between the large larger diameter portion 37a 1, 37a 2 of the relatively diameter Has a small diameter portion 37c having a small diameter. Large-diameter portion 37a 1, 37 2, respectively, the end portions of the first holding member 34 and 36 of the second dispensing fitment 31 and 32 are configured to be inserted. The diameters of the large diameter portions 37a 1 and 37a 2 are smaller than those of the hubs 34a and 36a which are the maximum diameter portions of the holding members 34 and 36.
【００３７】なお、本実施例では、小径部３７ｃは、大
径部３７ａ1 ，３７ａ2 よりも径が小さくされている
が、小径部３７ｃを設ける必要は必ずしもなく、貫通孔
３７ａは、その全体が同じ大きさの径とされていてもよ
い。In this embodiment, the small-diameter portion 37c has a smaller diameter than the large-diameter portions 37a 1 and 37a 2. However, the small-diameter portion 37c is not necessarily provided, and the through-hole 37a is formed as a whole. May have the same diameter.
【００３８】第３の実施例に係る分注方法を図１０を参
照して説明する。図１０に示すように、液状試料５が収
納されており、内部が第１の圧力されている第１の試験
管６を用意する。第１の試験管６は、栓体７で閉栓され
ている。この試験管６の栓体７に、第１の分注器具３１
の針管３３を貫通させる。そして、第１の分注器具３１
に、コネクタ３７を接続する。この場合、第１の分注器
具３１の保持部材３４の端部をコネクタ３７の貫通孔３
７ａのうち一方の大径部３７ａ1 に挿入することにより
接続が行われる。コネクタ３７の他端には、図８（ｂ）
に示した第２の分注器具３２の第２の保持部材３６を接
続する。A dispensing method according to the third embodiment will be described with reference to FIG. As shown in FIG. 10, a first test tube 6 containing a liquid sample 5 and having a first pressure therein is prepared. The first test tube 6 is closed with a stopper 7. A first dispensing device 31 is attached to the stopper 7 of the test tube 6.
Through the needle tube 33. Then, the first dispensing device 31
To the connector 37. In this case, the end of the holding member 34 of the first dispensing instrument 31 is connected to the through hole 3 of the connector 37.
Connection is made by inserting the large diameter portion 37a 1 of one of 7a. FIG. 8B shows the other end of the connector 37.
Is connected to the second holding member 36 of the second dispensing device 32 shown in FIG.
【００３９】しかる後、試験管６に第１の分注器具３１
及びコネクタ３７を接続した後に、全体を上下転倒し、
図１０に示されているように、第２の分注器具３２の針
管３５の針先３５ａを、内部が第２の圧力とされている
第２の試験管８の栓体９に挿入する。上記操作により、
第１，第２の試験管６，８が、第１，第２の分注器具３
１，３２及びコネクタ３７に連通されることになる。従
って、第１の実施例の場合と同様に、第１の圧力と第２
の圧力との圧力差により、液状試料５が第１の試験管６
から第２の試験管８に圧力差に応じた量だけ確実に移動
される。Thereafter, the first pipetting device 31 is placed in the test tube 6.
And after connecting the connector 37, the whole is turned upside down,
As shown in FIG. 10, the needle tip 35a of the needle tube 35 of the second dispensing instrument 32 is inserted into the stopper 9 of the second test tube 8 whose inside is at the second pressure. By the above operation,
The first and second test tubes 6 and 8 are the first and second dispensing instruments 3
1, 32 and the connector 37. Accordingly, as in the case of the first embodiment, the first pressure and the second pressure
Due to the pressure difference from the pressure of the first test tube 6
Is reliably moved to the second test tube 8 by an amount corresponding to the pressure difference.
【００４０】よって、第１，第２の分注器具３１，３２
及びコネクタ３７を用いることにより、外気と遮断した
状態で第１の試験管６内の液状試料５を確実に第２の試
験管８側に移動させることができる。Therefore, the first and second dispensing devices 31, 32
In addition, by using the connector 37, the liquid sample 5 in the first test tube 6 can be reliably moved to the second test tube 8 side in a state of being cut off from the outside air.
【００４１】上記コネクタ３７に代えて、図１１に示す
三方コック４０を用い、それによって第１，第２の試験
管の連通をオン・オフし得るように構成してもよい。こ
の場合には、三方コック４０が連通をオフする状態とし
ておいて、第１，第２の試験管にそれぞれ第１，第２の
分注器具３１，３２を接続し、かつ第１，第２の分注器
具３１，３２を三方コック４０で接続する。次に、三方
コック４０を連通オン状態すれば、容易にかつ確実に第
１の試験管６から第２の試験管８に液状試料を分注する
ことができる。Instead of the connector 37, a three-way cock 40 shown in FIG. 11 may be used so that the communication between the first and second test tubes can be turned on and off. In this case, with the three-way cock 40 in a state where the communication is turned off, the first and second dispensing devices 31 and 32 are connected to the first and second test tubes, respectively, and the first and second dispensing devices are connected. Are connected by a three-way cock 40. Next, if the three-way cock 40 is turned on, the liquid sample can be easily and reliably dispensed from the first test tube 6 to the second test tube 8.
【００４２】なお、このような連通制御機構を有するコ
ネクタとしては、三方コック４０の他、二方コックなど
様々な他の連通制御構造を有する接続部材を用いること
ができる。As the connector having such a communication control mechanism, in addition to the three-way cock 40, a connection member having various other communication control structures such as a two-way cock can be used.
【００４３】また、第３の実施例では、筒状のコネクタ
３７を用いたが、コネクタとしては、可撓性を有する接
続チューブを用いてもよい。次に、具体的な実験例につ
き説明する。Although the cylindrical connector 37 is used in the third embodiment, a flexible connection tube may be used as the connector. Next, specific experimental examples will be described.
【００４４】上述した第１の実施例の分注方法に従っ
て、分注操作を行った。まず、第１の試験管６として、
５ｍＬのプラスチック試験管を用意し、該プラスチック
試験管６に４Ｎの硫酸２ｍｌを添加し、ゴム栓からなる
栓体７により閉栓した。The dispensing operation was performed according to the dispensing method of the first embodiment described above. First, as the first test tube 6,
A 5 mL plastic test tube was prepared, 2 ml of 4N sulfuric acid was added to the plastic test tube 6, and the plastic test tube 6 was closed with a stopper 7 composed of a rubber stopper.
【００４５】他方、第２の試験管８として、５ｍＬのプ
ラスチック試験管にゴム栓からなる栓体９を載せ、減圧
条件下で打栓し、第２の試験管８内を減圧した。減圧条
件は、分注器具２の針先３ａを蒸留水に浸漬し、針先３
ｂを栓体９に刺通させた状態で３０秒放置した場合の吸
水量が２ｍＬとなるように調整した。On the other hand, as a second test tube 8, a plug 9 made of a rubber stopper was placed on a 5 mL plastic test tube, which was stoppered under reduced pressure, and the inside of the second test tube 8 was depressurized. The decompression condition is such that the needle tip 3a of the dispensing device 2 is immersed in distilled water,
The water absorption was adjusted to be 2 mL in the case where b was pierced through the plug 9 and left for 30 seconds.
【００４６】１Ｎの硫酸が収納された第１の試験管６の
栓体に、分注器具２の針先３ａを刺通し、上下逆転させ
た状態で、針先３ｂを減圧された第２の試験管８の栓体
９に刺通し、３０秒間放置した。しかる後、栓体９から
針先３ｂを抜去し、かつ第１の試験管６側からも針先３
ａを抜去し、第１，第２の試験管６，８の重量を測定し
た。第１，第２の試験管の分注前の重量と、分注後の重
量との比較から、分注量を求めた。上記分注操作を５回
繰り返し、分注量のばらつきを確かめた。結果を下記の
表１に示す。The second test in which the needle tip 3a of the dispensing instrument 2 was pierced into the stopper of the first test tube 6 containing 1N sulfuric acid and turned upside down, and the pressure of the needle tip 3b was reduced. The tube 8 was pierced through the plug 9 and left for 30 seconds. Thereafter, the needle tip 3b is removed from the plug 9, and the needle tip 3b is also removed from the first test tube 6 side.
a was removed, and the weights of the first and second test tubes 6 and 8 were measured. The dispensed amount was determined by comparing the weight of the first and second test tubes before dispensing with the weight after dispensing. The above dispensing operation was repeated five times, and the dispersion of the dispensed amount was confirmed. The results are shown in Table 1 below.
【００４７】[0047]
【表１】 [Table 1]
【００４８】表１から明らかなように、第２の試験管内
の圧力を上記のように設定することにより、２ｍｌの硫
酸を、第１，第２の試験管に均等に分離し得ることがわ
かる。As is evident from Table 1, by setting the pressure in the second test tube as described above, 2 ml of sulfuric acid can be equally separated into the first and second test tubes. .
【００４９】従って、本実施例によれば、外部との接触
を遮断した状態で一定量の液状試料を第１の試験管から
第２の試験管から安定に分注し得ることがわかる。次
に、上記第１の実施例の分注方法に従って、ただし試料
としてヒト血液を用いて分注操作を行った。すなわち、
５ｍＬのヘパリン含有プラスチック製真空採血管（積水
化学社製、２ｍＬ採血用）を用い、ヒト血液を採血し
た。この採血管の栓体に分注器具２の一方の針先３ａを
刺通し、反転させた状態で、別途用意した５ｍＬ用のヘ
パリン含有プラスチック製真空採血管（積水化学社製、
２ｍＬ採血用）の栓体に針先３ｂを刺通し、３０秒間放
置し、血液を分注した。Therefore, according to the present embodiment, it can be seen that a fixed amount of the liquid sample can be stably dispensed from the first test tube to the second test tube while the contact with the outside is cut off. Next, a dispensing operation was performed according to the dispensing method of the first embodiment, except that human blood was used as a sample. That is,
Human blood was collected using a 5 mL heparin-containing plastic vacuum blood collection tube (manufactured by Sekisui Chemical Co., Ltd., for 2 mL blood collection). One needle tip 3a of the dispensing instrument 2 is pierced into the stopper of the blood collection tube, and a 5 mL heparin-containing plastic vacuum blood collection tube (Sekisui Chemical Co., Ltd.
The needle tip 3b was pierced through the stopper (for collecting 2 mL of blood), left for 30 seconds, and the blood was dispensed.
【００５０】分注終了後、針を抜き、各採血管の重量を
測定し、分注前の採血管重量との比較から分注量を算出
した。なお、本試験においては、試験数ｎ＝５とした。
結果を下記の表２に示す。After the end of dispensing, the needle was withdrawn, the weight of each blood collection tube was measured, and the amount dispensed was calculated by comparison with the weight of the blood collection tube before dispensing. In this test, the number of tests was n = 5.
The results are shown in Table 2 below.
【００５１】[0051]
【表２】 [Table 2]
【００５２】表２から明らかなように、真空採血管を２
本用い、本実施例の分注器具２を利用することにより、
外部との接触を遮断した状態でヒト血液を高精度に分注
し得ることがわかる。As is clear from Table 2, the vacuum blood collection tube was
By using this and using the dispensing device 2 of this embodiment,
It can be seen that human blood can be dispensed with high precision in a state in which contact with the outside is cut off.
【００５３】[0053]
【発明の効果】第１の発明によれば、両端に針先を有
し、一端の針先が第１の試験管の栓体に貫通され、他端
の針先が第２の試験管の栓体に貫通させる針管と、針管
の長さ方向の途中に固定された保持部材とを備えるの
で、内部が第１の圧力とされて栓体により閉栓されてい
る第１の試験管に一端の針先を貫通し、しかる後内部が
第１の圧力よりも低い第２の圧力とされて栓体により閉
栓されている第２の試験管に他端の針先を挿通すること
により、液状試料が第１，第２の圧力の圧力差により第
１の試験管から第２の試験管に移動される。この場合液
状試料の移動量は、第１，第２の圧力差によって調整さ
れる。According to the first aspect of the present invention, a needle tip is provided at both ends, one end of the needle penetrates the plug of the first test tube, and the other end of the second test tube. Since a needle tube penetrating through the stopper and a holding member fixed in the middle of the length of the needle tube are provided, the first pressure is applied to the inside of the first test tube which is closed by the stopper at the first pressure. The liquid sample is penetrated through the needle tip and then the other end of the needle is inserted through a second test tube whose inside is set to a second pressure lower than the first pressure and closed by a stopper. Is moved from the first test tube to the second test tube by the pressure difference between the first and second pressures. In this case, the moving amount of the liquid sample is adjusted by the first and second pressure differences.
【００５４】従って、外気と液状の試料を遮断した状態
で、第１の試験管から第２の試験管に、液状試料の一部
を容易にかつ高精度に分注することが可能となる。第２
の発明に係る分注システムでは、第１の発明に係る分注
器具と筒状ホルダーとを備えているので、分注に際し第
１の試験管を筒状ホルダーに挿入し、筒状ホルダーの保
持孔に分注器具の保持部材を係合することにより、分注
操作をより安定に行うことが可能となる。Therefore, a part of the liquid sample can be easily and accurately dispensed from the first test tube to the second test tube while the outside air and the liquid sample are shut off. Second
In the dispensing system according to the invention, since the dispensing device and the cylindrical holder according to the first invention are provided, the first test tube is inserted into the cylindrical holder when dispensing, and the cylindrical holder is held. The dispensing operation can be performed more stably by engaging the holding member of the dispensing instrument with the hole.
【００５５】第３の発明に係る分注システムでは、第１
の針管及び筒状の第１の保持部材を備える第１の分注器
具と、第２の針管及び第２の保持部材を備える第２の分
注器具と、コネクタとを有するので、第１の試験管の栓
体に第１の分注器具の針先を貫通させ、コネクタにより
第１の分注器具及び第２の分注器具を接続し、第２の分
注器具の第２の針管の針先を第２の試験管の栓体に貫通
させることにより、第１の試験管から第２の試験管に、
第１，第２の圧力の圧力差に応じて液状試料を確実に移
動させることができる。従って、外気と遮断した状態
で、液状試料を第１の試験管から第２の試験管に容易に
かつ安全に移動させることができる。In the dispensing system according to the third invention, the first
A first dispensing device including a needle tube and a cylindrical first holding member, a second dispensing device including a second needle tube and a second holding member, and a connector. The needle of the first dispensing device is passed through the stopper of the test tube, the first dispensing device and the second dispensing device are connected by the connector, and the second needle tube of the second dispensing device is connected. By penetrating the needle tip through the stopper of the second test tube, the first test tube can be connected to the second test tube.
The liquid sample can be reliably moved according to the pressure difference between the first and second pressures. Therefore, the liquid sample can be easily and safely moved from the first test tube to the second test tube in a state where the liquid sample is shielded from the outside air.
【００５６】第３の発明に係る分注システムにおいて、
コネクタが連通制御機構を有する場合には、第１，第２
の分注器具及びコネクタを用いて第１，第２の試験管を
接続した状態を確認してから連通制御機構を操作するこ
とにより容易にかつ確実に第１の試験管から第２の試験
管側に液状試料を分注することができる。In the dispensing system according to the third invention,
If the connector has a communication control mechanism, the first and second
The first and second test tubes are easily and reliably connected to the first and second test tubes by operating the communication control mechanism after confirming the connected state of the first and second test tubes using the dispensing instrument and the connector. A liquid sample can be dispensed on the side.
【００５７】本発明に係る分注方法では、液状試料が収
納されており、内部が第１の圧力とされている第１の試
験管から内部が第１の圧力よりも低い第２の圧力とされ
た第２の試験管に、第１，第２の試験管を連通させた状
態で両者の圧力差により液状試料の一部が移動されるの
で、液状試料を外気と遮断した状態で、安全にかつ安定
に第１の試験管から第２の試験管に移動させることがで
きる。この場合、液状試料の移動量は、第１，第２の圧
力の圧力差に依存するので、圧力差を調整することによ
り、高精度に液状試料の一部を分注することが可能とな
る。[0057] In the dispensing method according to the present invention, the liquid sample is accommodated, and the first test tube whose inside is at the first pressure has a second pressure lower than the first pressure. A part of the liquid sample is moved by the pressure difference between the first and second test tubes while the first and second test tubes are in communication with the second test tube. It can be moved from the first test tube to the second test tube quickly and stably. In this case, since the moving amount of the liquid sample depends on the pressure difference between the first and second pressures, it is possible to dispense a part of the liquid sample with high accuracy by adjusting the pressure difference. .
【００５８】本発明に係る分注器具、分注システム及び
分注方法は、液状試料を外気と遮断した状態で分注する
ことを可能とする。従って、液状試料として、血液感染
の恐れがある血液試料を用いた場合に好適に利用するこ
とができる。また、血液試料だけでなく、毒物などの人
体や環境に汚染等を引き起こす様々な液状試料の分注に
も本発明を好適に用いることができる。The dispensing device, the dispensing system and the dispensing method according to the present invention enable dispensing a liquid sample in a state in which it is isolated from the outside air. Therefore, the present invention can be suitably used when a blood sample which may cause blood infection is used as the liquid sample. In addition, the present invention can be suitably used for dispensing not only blood samples but also various liquid samples that cause contamination of the human body and environment such as toxic substances.
【図面の簡単な説明】[Brief description of the drawings]
【図１】本発明の一実施例に係る分注器具を用いた分注
方法を説明するための縦断面図。FIG. 1 is a longitudinal sectional view for explaining a dispensing method using a dispensing device according to one embodiment of the present invention.
【図２】（ａ）及び（ｂ）は、本発明の一実施例に係る
分注システムに用いられる筒状ホルダー及び分注器具を
示す図であり、（ａ）は筒状ホルダーを示す断面図、
（ｂ）は分注器具を示す正面図。FIGS. 2A and 2B are views showing a cylindrical holder and a dispensing instrument used in a dispensing system according to one embodiment of the present invention, and FIG. Figure,
(B) is a front view showing a dispensing instrument.
【図３】本発明の一実施例に係る分注方法で用意される
第１の試験管及び液状試料を説明するための縦断面図。FIG. 3 is a longitudinal sectional view for explaining a first test tube and a liquid sample prepared by a dispensing method according to one embodiment of the present invention.
【図４】本発明の第１の実施例に係る分注方法におい
て、第１の試験管に筒状ホルダー及び分注器具を取り付
けた状態を示す縦断面図。FIG. 4 is a longitudinal sectional view showing a state in which a cylindrical holder and a dispensing device are attached to a first test tube in the dispensing method according to the first embodiment of the present invention.
【図５】第２の実施例に係る分注器具を示す正面図。FIG. 5 is a front view showing a dispensing device according to a second embodiment.
【図６】第２の実施例に係る分注器具を用いた分注方法
を説明するための縦断面図。FIG. 6 is a longitudinal sectional view for explaining a dispensing method using a dispensing device according to a second embodiment.
【図７】本発明の一実施例に係る分注器具の変形例を説
明するための縦断面図。FIG. 7 is a longitudinal sectional view for explaining a modification of the dispensing device according to one embodiment of the present invention.
【図８】（ａ）及び（ｂ）は、本発明の第３の実施例に
係る分注器具を示す正面図であり、（ａ）は第１の分注
器具を示し、（ｂ）は第２の分注器具を示す。FIGS. 8A and 8B are front views showing a dispensing device according to a third embodiment of the present invention, wherein FIG. 8A shows a first dispensing device, and FIG. 3 shows a second dispensing device.
【図９】（ａ）及び（ｂ）は、第３の実施例で用いられ
るコネクタを示す断面図及び斜視図。FIGS. 9A and 9B are a cross-sectional view and a perspective view showing a connector used in a third embodiment.
【図１０】第３の実施例に係る分注器具を用いた分注方
法を説明するための縦断面図。FIG. 10 is a longitudinal sectional view for explaining a dispensing method using a dispensing device according to a third embodiment.
【図１１】連通制御機構を有するコネクタとしての三方
コックを示す斜視図。FIG. 11 is a perspective view showing a three-way cock as a connector having a communication control mechanism.
１…筒状ホルダー １ａ…開口 １ｅ…保持孔 ２…分注器具 ３…針管 ３ａ，３ｂ…針先 ４…保持部材 ４ａ…雄ねじ ５…液状試料 ６…第１の試験管 ７…栓体 ８…第２の試験管 ９…栓体 １２…分注器具 １４…保持部材 １４ａ，１４ｂ…雄ねじ ２１…分注器具 ３１，３２…第１，第２の分注器具 ３３，３５…第１，第２の針管 ３３ａ，３５ａ…針先 ３４，３６…第１，第２の保持部材 ３７…コネクタ ４０…連通制御機構を有するコネクタとしての三方コッ
クDESCRIPTION OF SYMBOLS 1 ... Cylindrical holder 1a ... Opening 1e ... Holding hole 2 ... Dispensing instrument 3 ... Needle tube 3a, 3b ... Needle tip 4 ... Holding member 4a ... Male screw 5 ... Liquid sample 6 ... First test tube 7 ... Plug 8 ... Second test tube 9 ... stopper 12 ... dispensing device 14 ... holding member 14a, 14b ... male screw 21 ... dispensing device 31, 32 ... first and second dispensing devices 33, 35 ... first and second Needle tubes 33a, 35a ... Needle tips 34, 36 ... First and second holding members 37 ... Connector 40 ... Three-way cock as a connector having a communication control mechanism
フロントページの続き (72)発明者 五十川 浩信 山口県新南陽市開成町4560 積水化学工業 株式会社内 Ｆターム(参考） 4C038 TA10 Continued on the front page (72) Inventor Hironobu Ishikawa 4560 Kaiseicho, Shinnanyo-shi, Yamaguchi Sekisui Chemical Co., Ltd. F-term (reference) 4C038 TA10
Claims (6)
１の圧力とされて栓体により閉成されている第１の試験
管から、第１の圧力よりも低い第２の圧力とされて栓体
により閉成されている第２の試験管に、液状の試料の一
部を第１の圧力と第２の圧力との圧力差を利用して移動
させる分注方法に用いられる分注器具であって、 両端に針先を有し、一端の針先が第１の試験管の栓体に
貫通され、他端の針先が第２の試験管の栓体に貫通され
る針管と、 前記針管の長さ方向の途中に固定された保持部材とを備
えることを特徴とする分注器具。1. A first test tube containing a liquid sample, the inside of which is set to a first pressure and closed by a plug, is supplied with a second pressure lower than the first pressure. The dispensing method used in the dispensing method in which a part of the liquid sample is moved to the second test tube closed by the stopper using the pressure difference between the first pressure and the second pressure. A needle device having a needle tip at both ends, a needle tip at one end being penetrated by a plug of a first test tube, and a needle tip at the other end being penetrated by a plug of a second test tube. And a holding member fixed in the lengthwise direction of the needle tube.
に該一端が開口されており、他端に前記保持部材が係合
される保持孔を有する筒状ホルダーとを備えることを特
徴とする分注システム。2. The dispensing device according to claim 1, wherein one end is opened to enable insertion of the first test tube from one end, and the holding member engages with the other end. And a cylindrical holder having a holding hole to be dispensed.
一端側に有する第１の針管と、第１の針管の他端側に固
定された筒状の第１の保持部材とを備える第１の分注器
具と、 第２の試験管の栓体に貫通される針先を一端側に有する
第２の針管と、第２の針管の他端側に固定された筒状の
第２の保持部材とを備える第２の分注器具と、 第１の分注器具の第１の保持部材と、第２の分注器具の
第２の保持部材とを接続するコネクタとを備えることを
特徴とする分注システム。3. A first needle tube having at one end a needle tip penetrated by a plug of the first test tube, and a first cylindrical holding member fixed to the other end of the first needle tube. A first dispensing device comprising: a second needle tube having a needle tip penetrated by the stopper of the second test tube on one end side; and a cylindrical shape fixed to the other end side of the second needle tube. A second dispensing device including a second holding member, a first holding member of the first dispensing device, and a connector for connecting the second holding member of the second dispensing device. A dispensing system comprising:
通をオン・オフし得る連通制御機構を有することを特徴
とする請求項３に記載の分注システム。4. The dispensing system according to claim 3, wherein the connector has a communication control mechanism capable of turning on and off communication between the first and second needle tubes.
１の圧力とされて栓体により閉成されている第１の試験
管と、内部が第１の圧力よりも低い第２の圧力とされて
栓体により閉成されており、第１，第２の圧力の圧力差
が、移動させる試料の量に応じて選ばれている第２の試
験管とを用意する工程と、 第１の試験管と第２の試験管とを連通し、第１の圧力と
第２の圧力との圧力差により液状試料の一部を第１の試
験管から第２の試験管に移動させる工程とを備えること
を特徴とする分注方法。5. A first test tube containing a liquid sample, the inside of which is set to a first pressure and closed by a stopper, and a second test tube whose inside is lower than the first pressure. A step of preparing a second test tube which is closed by a stopper as a pressure, and wherein the pressure difference between the first and second pressures is selected according to the amount of the sample to be moved; A step of communicating the first test tube and the second test tube and moving a part of the liquid sample from the first test tube to the second test tube by a pressure difference between the first pressure and the second pressure; A dispensing method characterized by comprising:
５に記載の分注方法。6. The dispensing method according to claim 5, wherein the liquid sample is a blood sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11070466A JP2000262497A (en) | 1999-03-16 | 1999-03-16 | Dispensing appliance, dispensing system and dispensing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11070466A JP2000262497A (en) | 1999-03-16 | 1999-03-16 | Dispensing appliance, dispensing system and dispensing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2000262497A true JP2000262497A (en) | 2000-09-26 |
Family
ID=13432338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11070466A Withdrawn JP2000262497A (en) | 1999-03-16 | 1999-03-16 | Dispensing appliance, dispensing system and dispensing method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2000262497A (en) |
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